Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028098.01.T01 | XP_024639554.1 | 97.638 | 254 | 6 | 0 | 1 | 254 | 1 | 254 | 0 | 510 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028098.01.T01 | A0A1P8B554 | 52.8 | 250 | 103 | 5 | 9 | 251 | 16 | 257 | 2.40E-82 | 250 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028098.01.T01 | A0A396HV36 | 97.638 | 254 | 6 | 0 | 1 | 254 | 1 | 254 | 0.0 | 510 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000984.01 | MsG0580028098.01 | 0.835668 | 1.467795e-56 | 1.807210e-53 |
| MsG0180005678.01 | MsG0580028098.01 | 0.828799 | 7.355659e-55 | 7.387886e-52 |
| MsG0580025970.01 | MsG0580028098.01 | 0.804546 | 2.050907e-49 | 1.060370e-46 |
| MsG0580028098.01 | MsG0580029739.01 | 0.820105 | 8.177356e-53 | 6.411808e-50 |
| MsG0580028098.01 | MsG0880043920.01 | 0.808223 | 3.442217e-50 | 1.957142e-47 |
| MsG0280006924.01 | MsG0580028098.01 | 0.804753 | 1.856803e-49 | 9.652503e-47 |
| MsG0280008751.01 | MsG0580028098.01 | 0.803111 | 4.073250e-49 | 2.029716e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028098.01.T01 | MTR_8g037890 | 52.439 | 246 | 110 | 3 | 9 | 253 | 11 | 250 | 2.34e-88 | 265 |
| MsG0580028098.01.T01 | MTR_5g022310 | 53.138 | 239 | 111 | 1 | 14 | 251 | 14 | 252 | 1.47e-87 | 260 |
| MsG0580028098.01.T01 | MTR_5g022310 | 53.138 | 239 | 111 | 1 | 14 | 251 | 14 | 252 | 2.32e-87 | 261 |
| MsG0580028098.01.T01 | MTR_8g056820 | 52.869 | 244 | 108 | 3 | 9 | 251 | 11 | 248 | 2.86e-87 | 260 |
| MsG0580028098.01.T01 | MTR_1g025420 | 51.793 | 251 | 114 | 5 | 8 | 254 | 5 | 252 | 6.67e-86 | 259 |
| MsG0580028098.01.T01 | MTR_1g025420 | 52.459 | 244 | 111 | 4 | 8 | 249 | 5 | 245 | 7.13e-86 | 256 |
| MsG0580028098.01.T01 | MTR_4g073720 | 54.435 | 248 | 97 | 6 | 8 | 249 | 2 | 239 | 2.04e-85 | 258 |
| MsG0580028098.01.T01 | MTR_3g114030 | 54.506 | 233 | 101 | 4 | 24 | 254 | 5 | 234 | 8.11e-85 | 253 |
| MsG0580028098.01.T01 | MTR_4g073730 | 52.846 | 246 | 110 | 4 | 9 | 250 | 5 | 248 | 4.38e-84 | 253 |
| MsG0580028098.01.T01 | MTR_8g075510 | 53.755 | 253 | 97 | 8 | 8 | 253 | 8 | 247 | 1.27e-83 | 250 |
| MsG0580028098.01.T01 | MTR_8g036215 | 52.893 | 242 | 106 | 4 | 8 | 249 | 4 | 237 | 1.84e-83 | 249 |
| MsG0580028098.01.T01 | MTR_4g073730 | 52.846 | 246 | 110 | 4 | 9 | 250 | 5 | 248 | 1.88e-83 | 253 |
| MsG0580028098.01.T01 | MTR_8g075550 | 54.274 | 234 | 101 | 3 | 25 | 254 | 27 | 258 | 2.91e-82 | 249 |
| MsG0580028098.01.T01 | MTR_8g075510 | 53.815 | 249 | 95 | 8 | 8 | 249 | 8 | 243 | 1.05e-81 | 248 |
| MsG0580028098.01.T01 | MTR_6g079580 | 50.407 | 246 | 112 | 4 | 9 | 250 | 1 | 240 | 1.32e-80 | 242 |
| MsG0580028098.01.T01 | MTR_1g021945 | 54.310 | 232 | 97 | 4 | 21 | 250 | 21 | 245 | 1.29e-79 | 240 |
| MsG0580028098.01.T01 | MTR_5g022350 | 49.597 | 248 | 111 | 5 | 6 | 252 | 11 | 245 | 2.76e-77 | 236 |
| MsG0580028098.01.T01 | MTR_8g107140 | 47.809 | 251 | 120 | 4 | 9 | 254 | 5 | 249 | 3.70e-76 | 231 |
| MsG0580028098.01.T01 | MTR_3g111620 | 51.082 | 231 | 104 | 5 | 26 | 254 | 27 | 250 | 2.21e-75 | 229 |
| MsG0580028098.01.T01 | MTR_2g069660 | 50.820 | 244 | 109 | 5 | 13 | 250 | 5 | 243 | 2.82e-75 | 229 |
| MsG0580028098.01.T01 | MTR_2g067980 | 47.656 | 256 | 123 | 5 | 4 | 254 | 2 | 251 | 1.42e-73 | 242 |
| MsG0580028098.01.T01 | MTR_3g068015 | 46.032 | 252 | 117 | 6 | 11 | 250 | 4 | 248 | 4.83e-73 | 223 |
| MsG0580028098.01.T01 | MTR_7g102380 | 47.755 | 245 | 116 | 6 | 14 | 252 | 5 | 243 | 5.48e-73 | 223 |
| MsG0580028098.01.T01 | MTR_1g062630 | 47.451 | 255 | 120 | 6 | 1 | 253 | 3 | 245 | 8.48e-73 | 223 |
| MsG0580028098.01.T01 | MTR_1g062350 | 48.413 | 252 | 116 | 6 | 3 | 252 | 5 | 244 | 1.17e-71 | 219 |
| MsG0580028098.01.T01 | MTR_6g009480 | 45.600 | 250 | 124 | 5 | 9 | 250 | 7 | 252 | 4.09e-71 | 219 |
| MsG0580028098.01.T01 | MTR_1g062590 | 48.971 | 243 | 112 | 6 | 10 | 250 | 10 | 242 | 4.65e-71 | 218 |
| MsG0580028098.01.T01 | MTR_1g062370 | 47.619 | 252 | 118 | 6 | 3 | 252 | 5 | 244 | 1.20e-70 | 217 |
| MsG0580028098.01.T01 | MTR_1g062660 | 48.954 | 239 | 110 | 6 | 20 | 252 | 12 | 244 | 4.41e-70 | 216 |
| MsG0580028098.01.T01 | MTR_1g062340 | 48.971 | 243 | 108 | 6 | 20 | 252 | 8 | 244 | 5.98e-70 | 215 |
| MsG0580028098.01.T01 | MTR_5g059200 | 48.261 | 230 | 106 | 5 | 25 | 254 | 28 | 244 | 7.38e-70 | 216 |
| MsG0580028098.01.T01 | MTR_2g068030 | 44.958 | 238 | 122 | 5 | 20 | 254 | 17 | 248 | 1.97e-69 | 214 |
| MsG0580028098.01.T01 | MTR_2g068655 | 45.259 | 232 | 120 | 4 | 25 | 254 | 30 | 256 | 1.97e-68 | 214 |
| MsG0580028098.01.T01 | MTR_7g062610 | 44.215 | 242 | 128 | 3 | 10 | 250 | 7 | 242 | 2.96e-68 | 211 |
| MsG0580028098.01.T01 | MTR_4g063630 | 42.083 | 240 | 130 | 7 | 14 | 249 | 17 | 251 | 1.27e-60 | 192 |
| MsG0580028098.01.T01 | MTR_1g062690 | 44.490 | 245 | 100 | 7 | 10 | 252 | 10 | 220 | 6.59e-57 | 181 |
| MsG0580028098.01.T01 | MTR_5g010640 | 38.760 | 258 | 128 | 7 | 1 | 250 | 1 | 236 | 1.10e-54 | 176 |
| MsG0580028098.01.T01 | MTR_8g096900 | 41.870 | 246 | 113 | 8 | 9 | 250 | 29 | 248 | 4.11e-54 | 175 |
| MsG0580028098.01.T01 | MTR_5g010635 | 43.478 | 230 | 103 | 7 | 23 | 250 | 21 | 225 | 5.82e-53 | 172 |
| MsG0580028098.01.T01 | MTR_2g067990 | 38.095 | 231 | 110 | 8 | 24 | 252 | 25 | 224 | 9.48e-41 | 149 |
| MsG0580028098.01.T01 | MTR_5g068670 | 80.247 | 81 | 14 | 1 | 63 | 143 | 12 | 90 | 4.64e-37 | 127 |
| MsG0580028098.01.T01 | MTR_5g022350 | 48.000 | 150 | 68 | 5 | 9 | 157 | 82 | 222 | 5.38e-37 | 130 |
| MsG0580028098.01.T01 | MTR_5g023850 | 36.313 | 179 | 107 | 3 | 13 | 188 | 13 | 187 | 9.13e-37 | 129 |
| MsG0580028098.01.T01 | MTR_2g067970 | 39.459 | 185 | 102 | 7 | 72 | 252 | 6 | 184 | 1.93e-35 | 134 |
| MsG0580028098.01.T01 | MTR_8g089020 | 45.385 | 130 | 62 | 3 | 110 | 239 | 33 | 153 | 9.83e-35 | 122 |
| MsG0580028098.01.T01 | MTR_2g063150 | 29.200 | 250 | 157 | 7 | 9 | 248 | 9 | 248 | 9.28e-31 | 115 |
| MsG0580028098.01.T01 | MTR_8g096910 | 47.015 | 134 | 61 | 4 | 8 | 140 | 5 | 129 | 1.64e-30 | 112 |
| MsG0580028098.01.T01 | MTR_7g076360 | 34.722 | 216 | 72 | 5 | 25 | 239 | 7 | 154 | 2.19e-30 | 111 |
| MsG0580028098.01.T01 | MTR_1g062390 | 32.245 | 245 | 143 | 9 | 14 | 250 | 9 | 238 | 5.42e-30 | 112 |
| MsG0580028098.01.T01 | MTR_8g096920 | 45.865 | 133 | 62 | 5 | 9 | 140 | 3 | 126 | 1.13e-26 | 102 |
| MsG0580028098.01.T01 | MTR_3g081550 | 43.434 | 99 | 51 | 4 | 109 | 206 | 15 | 109 | 1.31e-13 | 65.9 |
| MsG0580028098.01.T01 | MTR_1g062640 | 41.111 | 90 | 49 | 3 | 79 | 167 | 60 | 146 | 4.10e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028098.01.T01 | AT5G24620 | 69.636 | 247 | 73 | 2 | 6 | 250 | 5 | 251 | 2.41e-123 | 357 |
| MsG0580028098.01.T01 | AT5G24620 | 69.636 | 247 | 73 | 2 | 6 | 250 | 60 | 306 | 8.66e-123 | 358 |
| MsG0580028098.01.T01 | AT5G24620 | 69.636 | 247 | 73 | 2 | 6 | 250 | 81 | 327 | 9.84e-123 | 358 |
| MsG0580028098.01.T01 | AT4G38660 | 54.694 | 245 | 99 | 5 | 10 | 252 | 13 | 247 | 2.45e-85 | 258 |
| MsG0580028098.01.T01 | AT1G19320 | 54.694 | 245 | 99 | 5 | 10 | 249 | 9 | 246 | 7.31e-85 | 253 |
| MsG0580028098.01.T01 | AT1G75800 | 52.419 | 248 | 113 | 4 | 10 | 253 | 5 | 251 | 1.18e-84 | 256 |
| MsG0580028098.01.T01 | AT4G38660 | 55.319 | 235 | 94 | 4 | 19 | 252 | 1 | 225 | 1.21e-84 | 255 |
| MsG0580028098.01.T01 | AT1G75050 | 50.820 | 244 | 114 | 4 | 10 | 253 | 9 | 246 | 1.77e-84 | 252 |
| MsG0580028098.01.T01 | AT1G75030 | 52.459 | 244 | 108 | 6 | 10 | 251 | 7 | 244 | 7.97e-84 | 251 |
| MsG0580028098.01.T01 | AT4G24180 | 52.800 | 250 | 103 | 5 | 9 | 251 | 16 | 257 | 2.45e-83 | 250 |
| MsG0580028098.01.T01 | AT4G24180 | 52.157 | 255 | 102 | 6 | 9 | 251 | 6 | 252 | 8.77e-83 | 248 |
| MsG0580028098.01.T01 | AT4G24180 | 52.800 | 250 | 103 | 5 | 9 | 251 | 16 | 257 | 9.60e-83 | 250 |
| MsG0580028098.01.T01 | AT2G17860 | 50.598 | 251 | 118 | 6 | 8 | 254 | 5 | 253 | 9.99e-83 | 248 |
| MsG0580028098.01.T01 | AT4G24180 | 52.157 | 255 | 102 | 6 | 9 | 251 | 6 | 252 | 2.24e-82 | 249 |
| MsG0580028098.01.T01 | AT1G20030 | 53.017 | 232 | 106 | 3 | 24 | 253 | 1 | 231 | 1.01e-81 | 247 |
| MsG0580028098.01.T01 | AT1G20030 | 53.017 | 232 | 106 | 3 | 24 | 253 | 18 | 248 | 1.48e-81 | 247 |
| MsG0580028098.01.T01 | AT4G38670 | 50.593 | 253 | 115 | 6 | 1 | 250 | 2 | 247 | 1.89e-81 | 246 |
| MsG0580028098.01.T01 | AT4G38670 | 50.593 | 253 | 115 | 6 | 1 | 250 | 2 | 247 | 1.89e-81 | 246 |
| MsG0580028098.01.T01 | AT1G75040 | 52.479 | 242 | 105 | 6 | 10 | 250 | 7 | 239 | 1.96e-80 | 242 |
| MsG0580028098.01.T01 | AT4G38670 | 54.425 | 226 | 97 | 5 | 28 | 250 | 9 | 231 | 1.24e-79 | 241 |
| MsG0580028098.01.T01 | AT4G36010 | 53.617 | 235 | 100 | 5 | 25 | 253 | 22 | 253 | 2.12e-79 | 241 |
| MsG0580028098.01.T01 | AT4G36010 | 53.617 | 235 | 100 | 5 | 25 | 253 | 22 | 253 | 2.12e-79 | 241 |
| MsG0580028098.01.T01 | AT1G73620 | 50.870 | 230 | 107 | 3 | 25 | 254 | 41 | 264 | 1.08e-76 | 233 |
| MsG0580028098.01.T01 | AT1G18250 | 50.870 | 230 | 107 | 3 | 21 | 250 | 16 | 239 | 2.31e-76 | 231 |
| MsG0580028098.01.T01 | AT1G18250 | 51.316 | 228 | 105 | 3 | 23 | 250 | 19 | 240 | 3.23e-76 | 231 |
| MsG0580028098.01.T01 | AT4G24180 | 52.137 | 234 | 97 | 5 | 9 | 235 | 16 | 241 | 1.12e-74 | 230 |
| MsG0580028098.01.T01 | AT4G24180 | 51.464 | 239 | 96 | 6 | 9 | 235 | 6 | 236 | 1.58e-74 | 229 |
| MsG0580028098.01.T01 | AT1G77700 | 47.390 | 249 | 118 | 5 | 6 | 254 | 41 | 276 | 4.54e-72 | 223 |
| MsG0580028098.01.T01 | AT1G77700 | 50.222 | 225 | 99 | 5 | 25 | 249 | 60 | 271 | 1.22e-71 | 223 |
| MsG0580028098.01.T01 | AT1G77700 | 50.222 | 225 | 99 | 5 | 25 | 249 | 88 | 299 | 2.41e-71 | 223 |
| MsG0580028098.01.T01 | AT5G38280 | 43.852 | 244 | 134 | 3 | 10 | 251 | 9 | 251 | 1.58e-70 | 229 |
| MsG0580028098.01.T01 | AT5G38280 | 43.852 | 244 | 134 | 3 | 10 | 251 | 9 | 251 | 2.53e-70 | 228 |
| MsG0580028098.01.T01 | AT5G40020 | 44.828 | 232 | 120 | 4 | 23 | 252 | 25 | 250 | 2.74e-66 | 206 |
| MsG0580028098.01.T01 | AT5G02140 | 43.265 | 245 | 131 | 4 | 8 | 250 | 3 | 241 | 7.78e-66 | 205 |
| MsG0580028098.01.T01 | AT5G02140 | 43.265 | 245 | 131 | 4 | 8 | 250 | 3 | 241 | 1.36e-65 | 206 |
| MsG0580028098.01.T01 | AT2G28790 | 43.621 | 243 | 118 | 8 | 19 | 249 | 38 | 273 | 8.03e-62 | 196 |
| MsG0580028098.01.T01 | AT2G28790 | 43.621 | 243 | 118 | 8 | 19 | 249 | 13 | 248 | 9.13e-62 | 194 |
| MsG0580028098.01.T01 | AT4G11650 | 42.387 | 243 | 114 | 6 | 11 | 252 | 8 | 225 | 3.00e-56 | 180 |
| MsG0580028098.01.T01 | AT5G24620 | 73.333 | 120 | 31 | 1 | 132 | 250 | 15 | 134 | 6.57e-56 | 181 |
| MsG0580028098.01.T01 | AT4G36000 | 53.333 | 150 | 58 | 2 | 101 | 250 | 1 | 138 | 1.45e-52 | 170 |
| MsG0580028098.01.T01 | AT4G36000 | 53.333 | 150 | 58 | 2 | 101 | 250 | 1 | 138 | 1.45e-52 | 170 |
| MsG0580028098.01.T01 | AT4G18253 | 37.349 | 249 | 136 | 8 | 9 | 254 | 10 | 241 | 3.73e-47 | 157 |
| MsG0580028098.01.T01 | AT4G18250 | 38.618 | 246 | 135 | 7 | 9 | 252 | 43 | 274 | 1.59e-43 | 156 |
| MsG0580028098.01.T01 | AT1G70250 | 37.021 | 235 | 134 | 7 | 24 | 252 | 143 | 369 | 7.18e-41 | 149 |
| MsG0580028098.01.T01 | AT2G24810 | 54.321 | 81 | 33 | 1 | 169 | 249 | 116 | 192 | 3.62e-22 | 90.9 |
Find 72 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGGTGGTCGGGTCGGATA+TGG | 0.198485 | 5:+74563700 | MsG0580028098.01.T01:CDS |
| CCAACTACTTGAAGTTCTTT+AGG | 0.222397 | 5:-74564661 | None:intergenic |
| GTGCCAGGCCATATAGTATA+AGG | 0.296447 | 5:-74563594 | None:intergenic |
| ACAATTGCCCTTATACTATA+TGG | 0.322158 | 5:+74563586 | MsG0580028098.01.T01:CDS |
| GTTCTTCCAAGAGGTGTATA+TGG | 0.346946 | 5:+74563915 | MsG0580028098.01.T01:CDS |
| ATAAGGGCAATTGTTTGTTA+TGG | 0.359715 | 5:-74563577 | None:intergenic |
| ACATTTGATGCATCAGGAAT+TGG | 0.364757 | 5:+74563738 | MsG0580028098.01.T01:CDS |
| GAAAGCAAATTACTTGTTGC+TGG | 0.375622 | 5:-74564914 | None:intergenic |
| GCGAAACTAACAAGTGTTCC+AGG | 0.375761 | 5:+74563681 | MsG0580028098.01.T01:CDS |
| GAAAATTGTGGAATAGAAAG+AGG | 0.379647 | 5:-74564794 | None:intergenic |
| CCTGCACCGGCGAGTGTGCC+AGG | 0.417031 | 5:-74563609 | None:intergenic |
| CCTAAAGAACTTCAAGTAGT+TGG | 0.432152 | 5:+74564661 | MsG0580028098.01.T01:CDS |
| TTCCACCACAATCACCTGTT+TGG | 0.443088 | 5:-74563767 | None:intergenic |
| AGGGTGGTCGGGTCGGATAT+GGG | 0.445110 | 5:+74563701 | MsG0580028098.01.T01:CDS |
| GGAATTGGTAAATGCCAAAC+AGG | 0.445838 | 5:+74563753 | MsG0580028098.01.T01:CDS |
| ACCTGAGTCAAGCTGAAATC+CGG | 0.449343 | 5:-74563652 | None:intergenic |
| CTTCAAGTAGTTGGTGGTGA+TGG | 0.451149 | 5:+74564670 | MsG0580028098.01.T01:CDS |
| GGTTGCACATTTGATGCATC+AGG | 0.453710 | 5:+74563732 | MsG0580028098.01.T01:CDS |
| TATAGCTATGCATTTGATGA+TGG | 0.459307 | 5:+74564838 | MsG0580028098.01.T01:CDS |
| CCTGGCACACTCGCCGGTGC+AGG | 0.465335 | 5:+74563609 | MsG0580028098.01.T01:CDS |
| AATGCATAGCTATAAGCTCT+TGG | 0.466628 | 5:-74564829 | None:intergenic |
| AGTTTCCAAGGTACAACTCT+TGG | 0.481226 | 5:-74563495 | None:intergenic |
| TATGATGTTAGCATGGTTGA+TGG | 0.484691 | 5:+74563873 | MsG0580028098.01.T01:CDS |
| ACAAGTGTTCCAGGGTGGTC+GGG | 0.491388 | 5:+74563690 | MsG0580028098.01.T01:CDS |
| AAGAGTGCCTGTGAAGCATT+TGG | 0.492315 | 5:+74564718 | MsG0580028098.01.T01:CDS |
| AAGCGCTGGCGTGCCTGCAC+CGG | 0.494818 | 5:-74563622 | None:intergenic |
| TGCCCTTATACTATATGGCC+TGG | 0.498957 | 5:+74563591 | MsG0580028098.01.T01:CDS |
| GGATACCAAGGTAGTGTAGT+TGG | 0.499416 | 5:+74564691 | MsG0580028098.01.T01:CDS |
| ATCTGATCCAAATGCTTCAC+AGG | 0.501968 | 5:-74564725 | None:intergenic |
| TGCCAGGCCATATAGTATAA+GGG | 0.519757 | 5:-74563593 | None:intergenic |
| GGTCGGATATGGGCAAGGAC+TGG | 0.525560 | 5:+74563711 | MsG0580028098.01.T01:CDS |
| TTCATAAGCTTTGCAAGTGA+AGG | 0.527419 | 5:-74564869 | None:intergenic |
| GATACCAAGGTAGTGTAGTT+GGG | 0.531220 | 5:+74564692 | MsG0580028098.01.T01:CDS |
| ACCGGATTTCAGCTTGACTC+AGG | 0.535646 | 5:+74563651 | MsG0580028098.01.T01:CDS |
| ACGCCAGCGCTTCCATCGAC+CGG | 0.538664 | 5:+74563633 | MsG0580028098.01.T01:CDS |
| TTCGAGATTACAATTGGTCA+AGG | 0.540110 | 5:+74563834 | MsG0580028098.01.T01:CDS |
| AAAATTGTGGAATAGAAAGA+GGG | 0.540420 | 5:-74564793 | None:intergenic |
| AACAAGTGTTCCAGGGTGGT+CGG | 0.542032 | 5:+74563689 | MsG0580028098.01.T01:CDS |
| CTCTTGGAAGAACAAGCATT+GGG | 0.542828 | 5:-74563905 | None:intergenic |
| TAATGCCAAGAGTTGTACCT+TGG | 0.546094 | 5:+74563490 | None:intergenic |
| CCAATGCTTGTTCTTCCAAG+AGG | 0.547326 | 5:+74563906 | MsG0580028098.01.T01:CDS |
| GTAATCTCGAAAAGCGAAGT+AGG | 0.550359 | 5:-74563822 | None:intergenic |
| GGTCGGGTCGGATATGGGCA+AGG | 0.550426 | 5:+74563706 | MsG0580028098.01.T01:CDS |
| AATCCGGTCGATGGAAGCGC+TGG | 0.553204 | 5:-74563636 | None:intergenic |
| AAAGAACTTCAAGTAGTTGG+TGG | 0.553885 | 5:+74564664 | MsG0580028098.01.T01:CDS |
| CCTCTTGGAAGAACAAGCAT+TGG | 0.554818 | 5:-74563906 | None:intergenic |
| ACAAGCCTGCTTGAAAATTG+TGG | 0.557208 | 5:-74564806 | None:intergenic |
| ATGAAACTTAGGAGTTTCCA+AGG | 0.562908 | 5:-74563507 | None:intergenic |
| TCAAGCTGAAATCCGGTCGA+TGG | 0.566995 | 5:-74563645 | None:intergenic |
| GTGGAAGGCTTGAATGTGAT+GGG | 0.572404 | 5:+74563784 | MsG0580028098.01.T01:CDS |
| CATATCCGACCCGACCACCC+TGG | 0.573915 | 5:-74563699 | None:intergenic |
| AGATTATTATGATGTTAGCA+TGG | 0.578494 | 5:+74563866 | MsG0580028098.01.T01:CDS |
| TACACCCAACTACACTACCT+TGG | 0.579077 | 5:-74564696 | None:intergenic |
| GTGTTCCAGGGTGGTCGGGT+CGG | 0.582643 | 5:+74563694 | MsG0580028098.01.T01:CDS |
| GGTGGAAGGCTTGAATGTGA+TGG | 0.584750 | 5:+74563783 | MsG0580028098.01.T01:CDS |
| GCAAATTACTTGTTGCTGGT+AGG | 0.587286 | 5:-74564910 | None:intergenic |
| ATATGGCCTGGCACACTCGC+CGG | 0.601753 | 5:+74563603 | MsG0580028098.01.T01:CDS |
| TCAGATCAATACTGTTGCAG+TGG | 0.604244 | 5:+74564742 | MsG0580028098.01.T01:CDS |
| ATGATGTTAGCATGGTTGAT+GGG | 0.620089 | 5:+74563874 | MsG0580028098.01.T01:CDS |
| TTGTGGAATAGAAAGAGGGT+TGG | 0.620148 | 5:-74564789 | None:intergenic |
| AGCTTTGCAAGTGAAGGTGC+TGG | 0.629823 | 5:-74564863 | None:intergenic |
| ATCTCGAAAAGCGAAGTAGG+TGG | 0.633321 | 5:-74563819 | None:intergenic |
| AAATGCCAAACAGGTGATTG+TGG | 0.638370 | 5:+74563762 | MsG0580028098.01.T01:CDS |
| AAAAGTGCATGTAATGCCAC+AGG | 0.642253 | 5:+74563939 | MsG0580028098.01.T01:CDS |
| CGAAACTAACAAGTGTTCCA+GGG | 0.644539 | 5:+74563682 | MsG0580028098.01.T01:CDS |
| AGGCTTGAATGTGATGGGAA+TGG | 0.651114 | 5:+74563789 | MsG0580028098.01.T01:CDS |
| AAACAGGTGATTGTGGTGGA+AGG | 0.656353 | 5:+74563769 | MsG0580028098.01.T01:CDS |
| TGCCAAACAGGTGATTGTGG+TGG | 0.666932 | 5:+74563765 | MsG0580028098.01.T01:CDS |
| AATATCAGTCACACAACCTG+TGG | 0.671889 | 5:-74563955 | None:intergenic |
| GTTGGTGGTGATGGATACCA+AGG | 0.675674 | 5:+74564679 | MsG0580028098.01.T01:CDS |
| AACTAACAAGTGTTCCAGGG+TGG | 0.716985 | 5:+74563685 | MsG0580028098.01.T01:CDS |
| TGTGTGACTGATATTAACAG+AGG | 0.717233 | 5:+74563963 | MsG0580028098.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAATAATTACTAAATT+TGG | + | Chr5:74564372-74564391 | MsG0580028098.01.T01:intron | 10.0% |
| !! | AAAATAAAAAAAAAATTGTG+AGG | + | Chr5:74564468-74564487 | MsG0580028098.01.T01:intron | 10.0% |
| !!! | AAATGTATTCTTTAAAAAAA+AGG | - | Chr5:74564572-74564591 | None:intergenic | 10.0% |
| !!! | AATGTATTCTTTAAAAAAAA+GGG | - | Chr5:74564571-74564590 | None:intergenic | 10.0% |
| !!! | AATTTTTTTTGTTTTGTTTT+TGG | + | Chr5:74564094-74564113 | MsG0580028098.01.T01:intron | 10.0% |
| !!! | TAAAAAATTAACAATTTTAC+AGG | + | Chr5:74564634-74564653 | MsG0580028098.01.T01:intron | 10.0% |
| !!! | TTTTAATCAAAAATTTACTA+TGG | - | Chr5:74564619-74564638 | None:intergenic | 10.0% |
| !! | AAACAAAAAAAATGAAACTT+AGG | - | Chr5:74563521-74563540 | None:intergenic | 15.0% |
| !! | CAAAAGTTAAACATAATAAA+CGG | + | Chr5:74564176-74564195 | MsG0580028098.01.T01:intron | 15.0% |
| !! | CAAAATCTGATATATTTATA+AGG | - | Chr5:74564031-74564050 | None:intergenic | 15.0% |
| !! | TTTAACCCAAAAAATATTAT+AGG | - | Chr5:74563996-74564015 | None:intergenic | 15.0% |
| !!! | AACATTCTGTTTAAATTTTT+AGG | + | Chr5:74564496-74564515 | MsG0580028098.01.T01:intron | 15.0% |
| !!! | ACATTCTGTTTAAATTTTTA+GGG | + | Chr5:74564497-74564516 | MsG0580028098.01.T01:intron | 15.0% |
| !!! | ATAAGTCCTATAATATTTTT+TGG | + | Chr5:74563987-74564006 | MsG0580028098.01.T01:intron | 15.0% |
| !!! | TAAGTCCTATAATATTTTTT+GGG | + | Chr5:74563988-74564007 | MsG0580028098.01.T01:intron | 15.0% |
| !! | ACAAAACAAAAAAAATTGTG+AGG | - | Chr5:74564091-74564110 | None:intergenic | 20.0% |
| !! | GACATTCATATTAACTTAAA+GGG | + | Chr5:74564242-74564261 | MsG0580028098.01.T01:intron | 20.0% |
| !! | TGACATTCATATTAACTTAA+AGG | + | Chr5:74564241-74564260 | MsG0580028098.01.T01:intron | 20.0% |
| !!! | CAAAATTCGCTATATTTTTA+AGG | + | Chr5:74564528-74564547 | MsG0580028098.01.T01:intron | 20.0% |
| ! | AAAATTGTGGAATAGAAAGA+GGG | - | Chr5:74564796-74564815 | None:intergenic | 25.0% |
| ! | AGATTATTATGATGTTAGCA+TGG | + | Chr5:74563866-74563885 | MsG0580028098.01.T01:CDS | 25.0% |
| ! | AGTCCTTATAAAATAAAACG+AGG | + | Chr5:74564056-74564075 | MsG0580028098.01.T01:intron | 25.0% |
| !!! | ACACCTCGTTTTATTTTATA+AGG | - | Chr5:74564062-74564081 | None:intergenic | 25.0% |
| !!! | ATTTTAACCATCACATATGA+TGG | + | Chr5:74564588-74564607 | MsG0580028098.01.T01:intron | 25.0% |
| AACAAGACCCTAACTTAAAT+TGG | + | Chr5:74564427-74564446 | MsG0580028098.01.T01:intron | 30.0% | |
| AAGTTGTGTATTGAATGCAT+TGG | + | Chr5:74564327-74564346 | MsG0580028098.01.T01:intron | 30.0% | |
| ACAATTGCCCTTATACTATA+TGG | + | Chr5:74563586-74563605 | MsG0580028098.01.T01:CDS | 30.0% | |
| CACTCTTTAAGCACTTTAAA+TGG | + | Chr5:74564289-74564308 | MsG0580028098.01.T01:intron | 30.0% | |
| CTAACTTAAATTGGCCATTA+GGG | + | Chr5:74564436-74564455 | MsG0580028098.01.T01:intron | 30.0% | |
| GAAAATTGTGGAATAGAAAG+AGG | - | Chr5:74564797-74564816 | None:intergenic | 30.0% | |
| TATAGCTATGCATTTGATGA+TGG | + | Chr5:74564838-74564857 | MsG0580028098.01.T01:CDS | 30.0% | |
| ! | ATAAGGGCAATTGTTTGTTA+TGG | - | Chr5:74563580-74563599 | None:intergenic | 30.0% |
| ! | CTAATGGCCAATTTAAGTTA+GGG | - | Chr5:74564437-74564456 | None:intergenic | 30.0% |
| !!! | ATTTTGTTTTTGGTCCCTAA+TGG | - | Chr5:74564453-74564472 | None:intergenic | 30.0% |
| AAAGAACTTCAAGTAGTTGG+TGG | + | Chr5:74564664-74564683 | MsG0580028098.01.T01:CDS | 35.0% | |
| AATGCATAGCTATAAGCTCT+TGG | - | Chr5:74564832-74564851 | None:intergenic | 35.0% | |
| ACATTTGATGCATCAGGAAT+TGG | + | Chr5:74563738-74563757 | MsG0580028098.01.T01:CDS | 35.0% | |
| ATGAAACTTAGGAGTTTCCA+AGG | - | Chr5:74563510-74563529 | None:intergenic | 35.0% | |
| ATGATGTTAGCATGGTTGAT+GGG | + | Chr5:74563874-74563893 | MsG0580028098.01.T01:CDS | 35.0% | |
| CCAACTACTTGAAGTTCTTT+AGG | - | Chr5:74564664-74564683 | None:intergenic | 35.0% | |
| CCTAAAGAACTTCAAGTAGT+TGG | + | Chr5:74564661-74564680 | MsG0580028098.01.T01:CDS | 35.0% | |
| CCTAACTTAAATTGGCCATT+AGG | + | Chr5:74564435-74564454 | MsG0580028098.01.T01:intron | 35.0% | |
| TATGATGTTAGCATGGTTGA+TGG | + | Chr5:74563873-74563892 | MsG0580028098.01.T01:CDS | 35.0% | |
| TGTGTGACTGATATTAACAG+AGG | + | Chr5:74563963-74563982 | MsG0580028098.01.T01:CDS | 35.0% | |
| TTCATAAGCTTTGCAAGTGA+AGG | - | Chr5:74564872-74564891 | None:intergenic | 35.0% | |
| TTCGAGATTACAATTGGTCA+AGG | + | Chr5:74563834-74563853 | MsG0580028098.01.T01:CDS | 35.0% | |
| ! | CCTAATGGCCAATTTAAGTT+AGG | - | Chr5:74564438-74564457 | None:intergenic | 35.0% |
| ! | CTTTTACCATATACACCTCT+TGG | - | Chr5:74563924-74563943 | None:intergenic | 35.0% |
| !! | CTATTCCACAATTTTCAAGC+AGG | + | Chr5:74564801-74564820 | MsG0580028098.01.T01:CDS | 35.0% |
| !! | TCGCTTTTCGAGATTACAAT+TGG | + | Chr5:74563828-74563847 | MsG0580028098.01.T01:CDS | 35.0% |
| AAATGCCAAACAGGTGATTG+TGG | + | Chr5:74563762-74563781 | MsG0580028098.01.T01:CDS | 40.0% | |
| AATATCAGTCACACAACCTG+TGG | - | Chr5:74563958-74563977 | None:intergenic | 40.0% | |
| ACAAGCCTGCTTGAAAATTG+TGG | - | Chr5:74564809-74564828 | None:intergenic | 40.0% | |
| AGTTTCCAAGGTACAACTCT+TGG | - | Chr5:74563498-74563517 | None:intergenic | 40.0% | |
| ATCTGATCCAAATGCTTCAC+AGG | - | Chr5:74564728-74564747 | None:intergenic | 40.0% | |
| CGAAACTAACAAGTGTTCCA+GGG | + | Chr5:74563682-74563701 | MsG0580028098.01.T01:CDS | 40.0% | |
| CTCTTGGAAGAACAAGCATT+GGG | - | Chr5:74563908-74563927 | None:intergenic | 40.0% | |
| GATACCAAGGTAGTGTAGTT+GGG | + | Chr5:74564692-74564711 | MsG0580028098.01.T01:CDS | 40.0% | |
| GGAATTGGTAAATGCCAAAC+AGG | + | Chr5:74563753-74563772 | MsG0580028098.01.T01:CDS | 40.0% | |
| GGTCATTCCATCATATGTGA+TGG | - | Chr5:74564598-74564617 | None:intergenic | 40.0% | |
| GTAATCTCGAAAAGCGAAGT+AGG | - | Chr5:74563825-74563844 | None:intergenic | 40.0% | |
| TCAGATCAATACTGTTGCAG+TGG | + | Chr5:74564742-74564761 | MsG0580028098.01.T01:CDS | 40.0% | |
| TGCCAGGCCATATAGTATAA+GGG | - | Chr5:74563596-74563615 | None:intergenic | 40.0% | |
| TTGTGGAATAGAAAGAGGGT+TGG | - | Chr5:74564792-74564811 | None:intergenic | 40.0% | |
| ! | AAAAGTGCATGTAATGCCAC+AGG | + | Chr5:74563939-74563958 | MsG0580028098.01.T01:CDS | 40.0% |
| ! | GTTCTTCCAAGAGGTGTATA+TGG | + | Chr5:74563915-74563934 | MsG0580028098.01.T01:CDS | 40.0% |
| !! | AAAGAGGGTTGGCATGTTTT+TGG | - | Chr5:74564781-74564800 | None:intergenic | 40.0% |
| !! | AAAGTGCTTAAAGAGTGTCC+CGG | - | Chr5:74564286-74564305 | None:intergenic | 40.0% |
| AAATGTTAACAAGTGCCCCC+GGG | + | Chr5:74564265-74564284 | MsG0580028098.01.T01:intron | 45.0% | |
| AACTAACAAGTGTTCCAGGG+TGG | + | Chr5:74563685-74563704 | MsG0580028098.01.T01:CDS | 45.0% | |
| ACCTGAGTCAAGCTGAAATC+CGG | - | Chr5:74563655-74563674 | None:intergenic | 45.0% | |
| ATCTCGAAAAGCGAAGTAGG+TGG | - | Chr5:74563822-74563841 | None:intergenic | 45.0% | |
| CCAATGCTTGTTCTTCCAAG+AGG | + | Chr5:74563906-74563925 | MsG0580028098.01.T01:CDS | 45.0% | |
| CCTCTTGGAAGAACAAGCAT+TGG | - | Chr5:74563909-74563928 | None:intergenic | 45.0% | |
| GAAATGTTAACAAGTGCCCC+CGG | + | Chr5:74564264-74564283 | MsG0580028098.01.T01:intron | 45.0% | |
| GCGAAACTAACAAGTGTTCC+AGG | + | Chr5:74563681-74563700 | MsG0580028098.01.T01:CDS | 45.0% | |
| GGATACCAAGGTAGTGTAGT+TGG | + | Chr5:74564691-74564710 | MsG0580028098.01.T01:CDS | 45.0% | |
| GGTTGCACATTTGATGCATC+AGG | + | Chr5:74563732-74563751 | MsG0580028098.01.T01:CDS | 45.0% | |
| GTGCCAGGCCATATAGTATA+AGG | - | Chr5:74563597-74563616 | None:intergenic | 45.0% | |
| GTGGAAGGCTTGAATGTGAT+GGG | + | Chr5:74563784-74563803 | MsG0580028098.01.T01:CDS | 45.0% | |
| TACACCCAACTACACTACCT+TGG | - | Chr5:74564699-74564718 | None:intergenic | 45.0% | |
| TGCCCTTATACTATATGGCC+TGG | + | Chr5:74563591-74563610 | MsG0580028098.01.T01:CDS | 45.0% | |
| TTCCACCACAATCACCTGTT+TGG | - | Chr5:74563770-74563789 | None:intergenic | 45.0% | |
| ! | AAACAGGTGATTGTGGTGGA+AGG | + | Chr5:74563769-74563788 | MsG0580028098.01.T01:CDS | 45.0% |
| ! | AGGCTTGAATGTGATGGGAA+TGG | + | Chr5:74563789-74563808 | MsG0580028098.01.T01:CDS | 45.0% |
| !! | AAGAGTGCCTGTGAAGCATT+TGG | + | Chr5:74564718-74564737 | MsG0580028098.01.T01:CDS | 45.0% |
| !! | AAGTGCTTAAAGAGTGTCCC+GGG | - | Chr5:74564285-74564304 | None:intergenic | 45.0% |
| !! | AATGCTTAAAGAGTGCCCTG+GGG | + | Chr5:74564395-74564414 | MsG0580028098.01.T01:intron | 45.0% |
| !! | CTTCAAGTAGTTGGTGGTGA+TGG | + | Chr5:74564670-74564689 | MsG0580028098.01.T01:CDS | 45.0% |
| !! | GAATGCTTAAAGAGTGCCCT+GGG | + | Chr5:74564394-74564413 | MsG0580028098.01.T01:intron | 45.0% |
| !!! | TTTTTTTTTTATTTTGTTTT+TGG | - | Chr5:74564463-74564482 | None:intergenic | 5.0% |
| AACAAGTGTTCCAGGGTGGT+CGG | + | Chr5:74563689-74563708 | MsG0580028098.01.T01:CDS | 50.0% | |
| ACCGGATTTCAGCTTGACTC+AGG | + | Chr5:74563651-74563670 | MsG0580028098.01.T01:CDS | 50.0% | |
| GGTGGAAGGCTTGAATGTGA+TGG | + | Chr5:74563783-74563802 | MsG0580028098.01.T01:CDS | 50.0% | |
| TCAAGCTGAAATCCGGTCGA+TGG | - | Chr5:74563648-74563667 | None:intergenic | 50.0% | |
| TGCCAAACAGGTGATTGTGG+TGG | + | Chr5:74563765-74563784 | MsG0580028098.01.T01:CDS | 50.0% | |
| ! | AGCTTTGCAAGTGAAGGTGC+TGG | - | Chr5:74564866-74564885 | None:intergenic | 50.0% |
| !! | AGTGCTTAAAGAGTGTCCCG+GGG | - | Chr5:74564284-74564303 | None:intergenic | 50.0% |
| !! | ATGCTTAAAGAGTGCCCTGG+GGG | + | Chr5:74564396-74564415 | MsG0580028098.01.T01:intron | 50.0% |
| !! | GGAATGCTTAAAGAGTGCCC+TGG | + | Chr5:74564393-74564412 | MsG0580028098.01.T01:intron | 50.0% |
| !! | GTTGGTGGTGATGGATACCA+AGG | + | Chr5:74564679-74564698 | MsG0580028098.01.T01:CDS | 50.0% |
| ACAAGTGTTCCAGGGTGGTC+GGG | + | Chr5:74563690-74563709 | MsG0580028098.01.T01:CDS | 55.0% | |
| CTTGTTAACGAGTGCCCCCA+GGG | - | Chr5:74564413-74564432 | None:intergenic | 55.0% | |
| TCTTGTTAACGAGTGCCCCC+AGG | - | Chr5:74564414-74564433 | None:intergenic | 55.0% | |
| !! | GTGCTTAAAGAGTGTCCCGG+GGG | - | Chr5:74564283-74564302 | None:intergenic | 55.0% |
| AGGGTGGTCGGGTCGGATAT+GGG | + | Chr5:74563701-74563720 | MsG0580028098.01.T01:CDS | 60.0% | |
| ATATGGCCTGGCACACTCGC+CGG | + | Chr5:74563603-74563622 | MsG0580028098.01.T01:CDS | 60.0% | |
| GGTCGGATATGGGCAAGGAC+TGG | + | Chr5:74563711-74563730 | MsG0580028098.01.T01:CDS | 60.0% | |
| ! | AATCCGGTCGATGGAAGCGC+TGG | - | Chr5:74563639-74563658 | None:intergenic | 60.0% |
| ACGCCAGCGCTTCCATCGAC+CGG | + | Chr5:74563633-74563652 | MsG0580028098.01.T01:CDS | 65.0% | |
| CAGGGTGGTCGGGTCGGATA+TGG | + | Chr5:74563700-74563719 | MsG0580028098.01.T01:CDS | 65.0% | |
| CATATCCGACCCGACCACCC+TGG | - | Chr5:74563702-74563721 | None:intergenic | 65.0% | |
| GGTCGGGTCGGATATGGGCA+AGG | + | Chr5:74563706-74563725 | MsG0580028098.01.T01:CDS | 65.0% | |
| GTGTTCCAGGGTGGTCGGGT+CGG | + | Chr5:74563694-74563713 | MsG0580028098.01.T01:CDS | 65.0% | |
| !! | AAGCGCTGGCGTGCCTGCAC+CGG | - | Chr5:74563625-74563644 | None:intergenic | 70.0% |
| CCTGGCACACTCGCCGGTGC+AGG | + | Chr5:74563609-74563628 | MsG0580028098.01.T01:CDS | 75.0% | |
| ! | CCTGCACCGGCGAGTGTGCC+AGG | - | Chr5:74563612-74563631 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 74563492 | 74564927 | 74563492 | ID=MsG0580028098.01;Name=MsG0580028098.01 |
| Chr5 | mRNA | 74563492 | 74564927 | 74563492 | ID=MsG0580028098.01.T01;Parent=MsG0580028098.01;Name=MsG0580028098.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|1|2|0|254 |
| Chr5 | exon | 74563492 | 74563984 | 74563492 | ID=MsG0580028098.01.T01:exon:23787;Parent=MsG0580028098.01.T01 |
| Chr5 | exon | 74564656 | 74564927 | 74564656 | ID=MsG0580028098.01.T01:exon:23788;Parent=MsG0580028098.01.T01 |
| Chr5 | CDS | 74563492 | 74563984 | 74563492 | ID=MsG0580028098.01.T01:cds;Parent=MsG0580028098.01.T01 |
| Chr5 | CDS | 74564656 | 74564927 | 74564656 | ID=MsG0580028098.01.T01:cds;Parent=MsG0580028098.01.T01 |
| Gene Sequence |
| Protein sequence |
