AlfalfaGEDB | MsG0580025970.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025970.01

KEGG: -
GO: GO:0030247
PFAM: PF13947 PF14380

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025970.01.T01	AES96452.1	96.284	296	11	0	1	296	1	296	0	587

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025970.01.T01	G7K8A0	96.284	296	11	0	1	296	1	296	0.0	587

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000161.01	MsG0580025970.01	0.835881	1.296226e-56	1.606289e-53
MsG0180000984.01	MsG0580025970.01	0.884166	2.418406e-71	1.578921e-67
MsG0180004828.01	MsG0580025970.01	0.827717	1.340526e-54	1.304700e-51
MsG0180005221.01	MsG0580025970.01	0.807848	4.135729e-50	2.328201e-47
MsG0180005325.01	MsG0580025970.01	0.820365	7.130258e-53	5.630320e-50
MsG0180005655.01	MsG0580025970.01	0.804982	1.662746e-49	8.694580e-47
MsG0180005678.01	MsG0580025970.01	0.868833	4.866243e-66	1.785787e-62
MsG0180006183.01	MsG0580025970.01	0.827164	1.819962e-54	1.743023e-51
MsG0180006253.01	MsG0580025970.01	0.816135	6.465183e-52	4.543331e-49
MsG0280006851.01	MsG0580025970.01	0.840758	7.171603e-58	1.031731e-54

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025970.01.T01	MTR_5g037310	96.284	296	11	0	1	296	1	296	0.0	587
MsG0580025970.01.T01	MTR_5g037310	96.284	296	11	0	1	296	1	296	0.0	586
MsG0580025970.01.T01	MTR_8g090055	39.711	277	141	8	1	266	1	262	1.08e-55	182
MsG0580025970.01.T01	MTR_4g123700	31.276	243	151	8	27	266	34	263	1.24e-25	103
MsG0580025970.01.T01	MTR_4g105550	28.364	275	165	11	10	266	15	275	6.70e-24	99.4
MsG0580025970.01.T01	MTR_4g132450	30.000	260	149	11	23	266	34	276	1.33e-20	90.5
MsG0580025970.01.T01	MTR_2g023970	28.244	262	151	12	23	266	20	262	2.07e-18	84.3
MsG0580025970.01.T01	MTR_7g112440	27.404	208	126	9	20	222	15	202	3.43e-11	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025970.01.T01	AT1G10380	55.197	279	115	7	25	296	27	302	5.44e-97	288
MsG0580025970.01.T01	AT1G10380	60.241	249	91	6	25	268	27	272	1.25e-94	282
MsG0580025970.01.T01	AT3G17350	33.333	246	145	9	27	264	31	265	1.03e-24	100
MsG0580025970.01.T01	AT3G17350	33.333	246	145	9	27	264	31	265	1.41e-24	100
MsG0580025970.01.T01	AT5G50290	30.147	272	165	11	10	266	5	266	1.18e-22	95.9
MsG0580025970.01.T01	AT1G11915	26.421	299	181	12	10	288	14	293	3.47e-17	80.9

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Find 69 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAAAATGGTGAGAAG+AGG	0.550845	5:+28350266	None:intergenic
AAAGCTGGACCAAGATTAAC+CGG	0.476906	5:+28349759	None:intergenic
AAATCAAGAAGTTATGATGA+TGG	0.541719	5:-28349897	MsG0580025970.01.T01:CDS
AACAAATTGATCCTCAAAAG+TGG	0.460285	5:-28349674	MsG0580025970.01.T01:CDS
AACCTGGGCCGCTACCGAAA+GGG	0.526652	5:+28350177	None:intergenic
AAGCGAGGGTCGCCGCAACC+TGG	0.352039	5:+28350161	None:intergenic
AATAGGACACAAGAAATAAA+TGG	0.343228	5:+28348987	None:intergenic
AATCAAGCTTATGATCACCT+AGG	0.658302	5:-28349099	MsG0580025970.01.T01:three_prime_UTR
ACAAGAACATGTTGACATGG+TGG	0.661350	5:+28350028	None:intergenic
ACAATCTACTAAAGCGAATA+TGG	0.279440	5:+28349944	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAATTGATCCTCAAAAG+TGG	-	Chr5:28349574-28349593	MsG0580025970.01.T01:CDS	30.0%
ACAATCTACTAAAGCGAATA+TGG	+	Chr5:28349307-28349326	None:intergenic	30.0%
CATAGTCAATTGAGGTAATA+GGG	+	Chr5:28349173-28349192	None:intergenic	30.0%
GTGATGTTATGTATTCTTGT+AGG	-	Chr5:28349415-28349434	MsG0580025970.01.T01:intron	30.0%
TAAACACAACATGTCGAAAT+CGG	+	Chr5:28349465-28349484	None:intergenic	30.0%
AAAAGGAAAATGGTGAGAAG+AGG	+	Chr5:28348985-28349004	None:intergenic	35.0%
AATCAAGCTTATGATCACCT+AGG	-	Chr5:28350149-28350168	MsG0580025970.01.T01:CDS	35.0%
AGCAAACCCTGAAAACATTA+AGG	+	Chr5:28350066-28350085	None:intergenic	35.0%
AGGAAGTAGGAAAAGGAAAA+TGG	+	Chr5:28348995-28349014	None:intergenic	35.0%
ATGATCACCTAGGATTGTAA+AGG	-	Chr5:28350159-28350178	MsG0580025970.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	28348901	28350369	28348901	ID=MsG0580025970.01;Name=MsG0580025970.01
Chr5	mRNA	28348901	28350369	28348901	ID=MsG0580025970.01.T01;Parent=MsG0580025970.01;Name=MsG0580025970.01.T01;_AED=0.13;_eAED=0.13;_QI=57\|1\|1\|1\|1\|1\|2\|236\|296
Chr5	exon	28348901	28349192	28348901	ID=MsG0580025970.01.T01:exon:7498;Parent=MsG0580025970.01.T01
Chr5	exon	28349478	28350369	28349478	ID=MsG0580025970.01.T01:exon:7497;Parent=MsG0580025970.01.T01
Chr5	five_prime_UTR	28350313	28350369	28350313	ID=MsG0580025970.01.T01:five_prime_utr;Parent=MsG0580025970.01.T01
Chr5	CDS	28349478	28350312	28349478	ID=MsG0580025970.01.T01:cds;Parent=MsG0580025970.01.T01
Chr5	CDS	28349137	28349192	28349137	ID=MsG0580025970.01.T01:cds;Parent=MsG0580025970.01.T01
Chr5	three_prime_UTR	28348901	28349136	28348901	ID=MsG0580025970.01.T01:three_prime_utr;Parent=MsG0580025970.01.T01

Gene Sequence

>MsG0580025970.01.T01

ATGAAATTTAGTTACCCTTCACCTCCTCTTCTCACCATTTTCCTTTTCCTACTTCCTTCTTTGATCCAATCTCAAATGTGCCAAAGAAATTGTGGCAAAGAACCTCTCAAGTACCCTTTCGGTAGCGGCCCAGGTTGCGGCGACCCTCGCTTCCAACCACATATAACTTGCAGCCAACAAAAACTCACCTTCACAACACACACAGGCTCCTACCCTATTACCTCAATTGACTATGCAAATCAAGTCATATACATTTCAGATCCCACCATGTCAACATGTTCTTGTACCCTACCTAGCAAAGGCTTCGGCTTAGATTGGAACGCGCCTTTTACTTTCGATGATAGCACCATATTCGCTTTAGTAGATTGTTCGATGAATTCATCATCGATATGTAAATCAAGAAGTTATGATGATGGAAGCAATTCCAAGCTTCAATGTGATCAAAATACACAAATTTGTGATGTTATGTATTCTTGTAGGCCTATTAGTACTAATATTAACCTTCCGATTTCGACATGTTGTGTTTATACACCGGTTAATCTTGGTCCAGCTTTTGATATGGATCTTCAAAAGCTTCAATGCAGTTCATATACAGGTTTTTACAATTACAATGATCAACAAATTGATCCTCAAAAGTGGAACTATGGAATTGCACTTAAGTATAAGTTTAGTGTTACAAATGATTATCCTAGCTCTTGTGCTGCATGTGAAAGGAGCTTTGGATTTTGTGGATATAGTGAAGTTTATAATTCATTTTTATGCAACTGTCCTAATGGAATTAACACCACTACAGATTGTTTCTTCACATCATCTTATAATAATGGTTATAAAAATGGGTTTGCTTGGTTGATATATGCTTTTGCATGGTCTTTGATTAGGCTCATCTTATAA

Protein sequence

>MsG0580025970.01.T01

MKFSYPSPPLLTIFLFLLPSLIQSQMCQRNCGKEPLKYPFGSGPGCGDPRFQPHITCSQQKLTFTTHTGSYPITSIDYANQVIYISDPTMSTCSCTLPSKGFGLDWNAPFTFDDSTIFALVDCSMNSSSICKSRSYDDGSNSKLQCDQNTQICDVMYSCRPISTNINLPISTCCVYTPVNLGPAFDMDLQKLQCSSYTGFYNYNDQQIDPQKWNYGIALKYKFSVTNDYPSSCAACERSFGFCGYSEVYNSFLCNCPNGINTTTDCFFTSSYNNGYKNGFAWLIYAFAWSLIRLIL*