Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028529.01.T01 | XP_024638920.1 | 76.852 | 432 | 71 | 2 | 1 | 403 | 122 | 553 | 0 | 655 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028529.01.T01 | Q9FGH9 | 50.345 | 290 | 103 | 4 | 131 | 391 | 189 | 466 | 4.56E-84 | 271 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028529.01.T01 | G7JZR8 | 76.168 | 428 | 73 | 2 | 1 | 399 | 122 | 549 | 0.0 | 644 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280011446.01 | MsG0580028529.01 | 0.803937 | 2.746458e-49 | 1.398077e-46 |
| MsG0380014573.01 | MsG0580028529.01 | 0.822237 | 2.636881e-53 | 2.194474e-50 |
| MsG0380015877.01 | MsG0580028529.01 | 0.810441 | 1.151153e-50 | 6.939296e-48 |
| MsG0580028529.01 | MsG0680035573.01 | 0.823812 | 1.132374e-53 | 9.854481e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028529.01.T01 | MTR_5g073480 | 76.168 | 428 | 73 | 2 | 1 | 399 | 122 | 549 | 0.0 | 644 |
| MsG0580028529.01.T01 | MTR_5g073460 | 60.096 | 416 | 135 | 4 | 1 | 386 | 122 | 536 | 1.46e-162 | 473 |
| MsG0580028529.01.T01 | MTR_5g073620 | 42.377 | 446 | 177 | 6 | 1 | 386 | 122 | 547 | 5.50e-98 | 315 |
| MsG0580028529.01.T01 | MTR_5g073450 | 54.483 | 290 | 100 | 4 | 131 | 391 | 194 | 480 | 7.75e-97 | 303 |
| MsG0580028529.01.T01 | MTR_5g074130 | 40.191 | 418 | 193 | 13 | 1 | 384 | 123 | 517 | 1.12e-77 | 255 |
| MsG0580028529.01.T01 | MTR_3g031130 | 47.603 | 292 | 118 | 5 | 131 | 390 | 184 | 472 | 7.05e-70 | 231 |
| MsG0580028529.01.T01 | MTR_5g073920 | 36.165 | 412 | 212 | 10 | 1 | 384 | 119 | 507 | 6.81e-64 | 217 |
| MsG0580028529.01.T01 | MTR_5g073770 | 44.167 | 240 | 122 | 3 | 23 | 255 | 85 | 319 | 2.43e-56 | 190 |
| MsG0580028529.01.T01 | MTR_3g464010 | 35.897 | 429 | 199 | 14 | 1 | 386 | 120 | 515 | 4.08e-56 | 196 |
| MsG0580028529.01.T01 | MTR_5g073960 | 42.969 | 256 | 120 | 8 | 5 | 255 | 126 | 360 | 8.04e-52 | 179 |
| MsG0580028529.01.T01 | MTR_5g073470 | 47.179 | 195 | 69 | 1 | 1 | 195 | 98 | 258 | 3.44e-49 | 168 |
| MsG0580028529.01.T01 | MTR_5g073610 | 42.326 | 215 | 103 | 6 | 1 | 214 | 123 | 317 | 1.89e-42 | 152 |
| MsG0580028529.01.T01 | MTR_5g073820 | 32.918 | 401 | 182 | 9 | 1 | 386 | 100 | 428 | 3.49e-41 | 152 |
| MsG0580028529.01.T01 | MTR_5g073650 | 35.119 | 336 | 134 | 14 | 1 | 293 | 21 | 315 | 3.30e-38 | 140 |
| MsG0580028529.01.T01 | MTR_5g073810 | 36.398 | 261 | 108 | 7 | 1 | 243 | 123 | 343 | 4.71e-37 | 139 |
| MsG0580028529.01.T01 | MTR_5g073660 | 37.500 | 240 | 86 | 4 | 1 | 219 | 100 | 296 | 2.01e-36 | 135 |
| MsG0580028529.01.T01 | MTR_5g074450 | 60.156 | 128 | 35 | 2 | 1 | 112 | 122 | 249 | 2.64e-36 | 139 |
| MsG0580028529.01.T01 | MTR_5g093530 | 53.543 | 127 | 50 | 4 | 1 | 127 | 123 | 240 | 4.13e-29 | 119 |
| MsG0580028529.01.T01 | MTR_5g093530 | 31.837 | 245 | 125 | 6 | 170 | 384 | 205 | 437 | 5.11e-18 | 86.3 |
| MsG0580028529.01.T01 | MTR_4g103540 | 30.928 | 291 | 165 | 9 | 126 | 384 | 237 | 523 | 1.96e-28 | 117 |
| MsG0580028529.01.T01 | MTR_5g073750 | 45.783 | 166 | 83 | 5 | 1 | 165 | 122 | 281 | 1.66e-27 | 110 |
| MsG0580028529.01.T01 | MTR_8g021330 | 26.644 | 289 | 168 | 5 | 138 | 386 | 161 | 445 | 1.07e-26 | 112 |
| MsG0580028529.01.T01 | MTR_6g033310 | 27.273 | 308 | 176 | 6 | 125 | 384 | 259 | 566 | 2.95e-26 | 111 |
| MsG0580028529.01.T01 | MTR_1g090620 | 29.197 | 274 | 151 | 5 | 141 | 384 | 208 | 468 | 7.40e-25 | 107 |
| MsG0580028529.01.T01 | MTR_4g062330 | 29.565 | 230 | 132 | 4 | 123 | 322 | 155 | 384 | 4.64e-24 | 104 |
| MsG0580028529.01.T01 | MTR_5g073900 | 34.848 | 264 | 117 | 10 | 156 | 386 | 1 | 242 | 5.32e-24 | 102 |
| MsG0580028529.01.T01 | MTR_1g057560 | 27.007 | 274 | 159 | 3 | 141 | 385 | 202 | 463 | 1.44e-23 | 103 |
| MsG0580028529.01.T01 | MTR_3g025900 | 27.362 | 307 | 174 | 7 | 143 | 403 | 178 | 481 | 4.53e-22 | 99.0 |
| MsG0580028529.01.T01 | MTR_2g096230 | 24.567 | 289 | 167 | 8 | 153 | 398 | 172 | 452 | 2.88e-20 | 93.2 |
| MsG0580028529.01.T01 | MTR_7g013770 | 23.358 | 274 | 170 | 4 | 153 | 386 | 241 | 514 | 1.40e-17 | 85.1 |
| MsG0580028529.01.T01 | MTR_1g067480 | 23.239 | 284 | 173 | 6 | 138 | 380 | 166 | 445 | 3.02e-16 | 80.9 |
| MsG0580028529.01.T01 | MTR_1g067460 | 23.920 | 301 | 187 | 8 | 138 | 398 | 164 | 462 | 6.10e-15 | 77.0 |
| MsG0580028529.01.T01 | MTR_5g074190 | 64.912 | 57 | 20 | 0 | 1 | 57 | 116 | 172 | 9.38e-15 | 76.3 |
| MsG0580028529.01.T01 | MTR_1g117090 | 24.034 | 233 | 144 | 2 | 137 | 336 | 197 | 429 | 1.91e-14 | 75.5 |
| MsG0580028529.01.T01 | MTR_5g073840 | 48.193 | 83 | 42 | 1 | 1 | 83 | 122 | 203 | 7.44e-13 | 68.6 |
| MsG0580028529.01.T01 | MTR_5g073880 | 30.769 | 156 | 81 | 4 | 1 | 156 | 123 | 251 | 1.19e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028529.01.T01 | AT5G58430 | 50.345 | 290 | 103 | 4 | 131 | 391 | 189 | 466 | 4.65e-85 | 271 |
| MsG0580028529.01.T01 | AT1G07000 | 39.384 | 292 | 134 | 4 | 131 | 391 | 171 | 450 | 5.57e-53 | 186 |
| MsG0580028529.01.T01 | AT1G07725 | 27.509 | 269 | 152 | 5 | 153 | 384 | 184 | 446 | 1.19e-27 | 115 |
| MsG0580028529.01.T01 | AT5G03540 | 29.643 | 280 | 157 | 4 | 141 | 391 | 87 | 355 | 7.04e-27 | 112 |
| MsG0580028529.01.T01 | AT5G03540 | 29.181 | 281 | 157 | 4 | 141 | 391 | 202 | 470 | 1.19e-26 | 112 |
| MsG0580028529.01.T01 | AT5G03540 | 29.181 | 281 | 157 | 4 | 141 | 391 | 228 | 496 | 1.25e-26 | 112 |
| MsG0580028529.01.T01 | AT5G50380 | 27.306 | 271 | 162 | 4 | 142 | 384 | 240 | 503 | 2.73e-26 | 111 |
| MsG0580028529.01.T01 | AT5G13150 | 27.817 | 284 | 169 | 4 | 144 | 391 | 210 | 493 | 3.97e-26 | 111 |
| MsG0580028529.01.T01 | AT1G72470 | 27.083 | 288 | 163 | 6 | 138 | 384 | 178 | 459 | 6.66e-26 | 110 |
| MsG0580028529.01.T01 | AT5G52340 | 27.106 | 273 | 158 | 4 | 141 | 384 | 202 | 462 | 2.54e-22 | 99.8 |
| MsG0580028529.01.T01 | AT3G14090 | 25.000 | 284 | 173 | 5 | 144 | 391 | 179 | 458 | 1.15e-21 | 97.8 |
| MsG0580028529.01.T01 | AT2G28640 | 24.028 | 283 | 176 | 4 | 139 | 384 | 150 | 430 | 1.21e-21 | 97.4 |
| MsG0580028529.01.T01 | AT1G54090 | 26.316 | 285 | 167 | 5 | 138 | 384 | 170 | 449 | 3.55e-21 | 96.3 |
| MsG0580028529.01.T01 | AT5G59730 | 27.027 | 296 | 176 | 8 | 143 | 398 | 164 | 459 | 1.78e-20 | 94.0 |
| MsG0580028529.01.T01 | AT5G59730 | 27.027 | 296 | 176 | 8 | 143 | 398 | 164 | 459 | 1.79e-20 | 94.0 |
| MsG0580028529.01.T01 | AT3G09520 | 25.991 | 227 | 133 | 2 | 131 | 322 | 155 | 381 | 9.25e-19 | 89.0 |
| MsG0580028529.01.T01 | AT5G13990 | 25.360 | 347 | 215 | 8 | 67 | 384 | 192 | 523 | 4.14e-18 | 87.0 |
| MsG0580028529.01.T01 | AT3G09530 | 27.723 | 202 | 114 | 3 | 153 | 322 | 174 | 375 | 2.23e-17 | 84.7 |
| MsG0580028529.01.T01 | AT3G55150 | 25.260 | 289 | 176 | 7 | 146 | 396 | 184 | 470 | 3.40e-16 | 80.9 |
| MsG0580028529.01.T01 | AT2G28650 | 23.596 | 267 | 160 | 4 | 153 | 384 | 156 | 413 | 1.54e-15 | 79.0 |
| MsG0580028529.01.T01 | AT5G61010 | 24.723 | 271 | 164 | 8 | 149 | 384 | 215 | 480 | 3.09e-15 | 78.2 |
| MsG0580028529.01.T01 | AT5G61010 | 24.723 | 271 | 164 | 8 | 149 | 384 | 215 | 480 | 3.09e-15 | 78.2 |
| MsG0580028529.01.T01 | AT5G52350 | 22.353 | 340 | 208 | 9 | 86 | 384 | 92 | 416 | 1.37e-13 | 72.8 |
| MsG0580028529.01.T01 | AT3G29400 | 23.929 | 280 | 163 | 7 | 153 | 384 | 212 | 489 | 4.92e-13 | 71.2 |
Find 93 sgRNAs with CRISPR-Local
Find 105 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAGTTTGAAGAAGATTT+TGG | 0.106248 | 5:+81889332 | MsG0580028529.01.T01:CDS |
| ATGCACTCTATCCTCAATTT+TGG | 0.137625 | 5:-81889375 | None:intergenic |
| CAGTTGCAGGAGGGAATATT+TGG | 0.209561 | 5:+81888757 | MsG0580028529.01.T01:CDS |
| AGTAGGGTCCTCGTAGATTT+TGG | 0.216151 | 5:-81889468 | None:intergenic |
| AAATCTTATCGGAATGCTTT+TGG | 0.224265 | 5:+81888574 | MsG0580028529.01.T01:CDS |
| CCGGAAACTGCTGCCTTTGC+TGG | 0.241109 | 5:-81889233 | None:intergenic |
| AAAAGCATTCCGATAAGATT+TGG | 0.270775 | 5:-81888572 | None:intergenic |
| CCAGCAAAGGCAGCAGTTTC+CGG | 0.273611 | 5:+81889233 | MsG0580028529.01.T01:CDS |
| GAATACCTTAGCGATGATTC+TGG | 0.302254 | 5:+81888608 | MsG0580028529.01.T01:CDS |
| GAAGCTATTAGAGAGCATTT+TGG | 0.306024 | 5:+81889436 | MsG0580028529.01.T01:CDS |
| TATCCTTCACGGATGTTTGC+AGG | 0.329769 | 5:+81888969 | MsG0580028529.01.T01:CDS |
| TTCAACACGTAATGGGTAAT+CGG | 0.348059 | 5:-81889269 | None:intergenic |
| CAAGAAAATCCAAATCTTAT+CGG | 0.350480 | 5:+81888563 | MsG0580028529.01.T01:CDS |
| GTAGAAAGCTTATCAAGATT+AGG | 0.355489 | 5:+81888779 | MsG0580028529.01.T01:CDS |
| CTTTCATCTTCTAAACTAGT+AGG | 0.359405 | 5:-81888437 | None:intergenic |
| ACAACAAAGCAACAACAATT+TGG | 0.361181 | 5:+81889148 | MsG0580028529.01.T01:CDS |
| TAGAAAGCTTATCAAGATTA+GGG | 0.361927 | 5:+81888780 | MsG0580028529.01.T01:CDS |
| AACAACAATTTGGAAGAGAT+TGG | 0.368141 | 5:+81889158 | MsG0580028529.01.T01:CDS |
| CACTGCTGATTTATCCTTCA+CGG | 0.374636 | 5:+81888958 | MsG0580028529.01.T01:CDS |
| TCACAAAACAGCAAAGTTGA+TGG | 0.385033 | 5:+81888694 | MsG0580028529.01.T01:CDS |
| CTGCTGCCTTTGCTGGATCT+TGG | 0.387509 | 5:-81889226 | None:intergenic |
| TCGACGTGTATATCAGTTGC+AGG | 0.391694 | 5:+81888744 | MsG0580028529.01.T01:CDS |
| ACAACAATTTGGAAGAGATT+GGG | 0.393701 | 5:+81889159 | MsG0580028529.01.T01:CDS |
| AAGGCTTTGATCATGAAGTC+TGG | 0.398018 | 5:-81888647 | None:intergenic |
| AAATTGCAGTTATTCATTGT+TGG | 0.402284 | 5:+81888338 | MsG0580028529.01.T01:CDS |
| TACTGATATTGGTAGTAGTA+TGG | 0.405413 | 5:+81888505 | MsG0580028529.01.T01:CDS |
| CAGTTATTCATTGTTGGAGC+AGG | 0.433635 | 5:+81888344 | MsG0580028529.01.T01:CDS |
| TGAACGGAAACTTTGTGATC+GGG | 0.445015 | 5:+81888916 | MsG0580028529.01.T01:CDS |
| AAGGTAGTTCAACACGTAAT+GGG | 0.457041 | 5:-81889276 | None:intergenic |
| AAGGCAGCAGTTTCCGGTGT+TGG | 0.464676 | 5:+81889239 | MsG0580028529.01.T01:CDS |
| TTCCTCTCTATCAGTGAAGA+TGG | 0.465906 | 5:+81889403 | MsG0580028529.01.T01:CDS |
| AGCCTTGCCAACTGAAACAA+TGG | 0.468338 | 5:+81888664 | MsG0580028529.01.T01:CDS |
| CAGTTGGGCAGTACTGATAT+TGG | 0.468719 | 5:+81888494 | MsG0580028529.01.T01:CDS |
| GAAGGTAGTTCAACACGTAA+TGG | 0.475458 | 5:-81889277 | None:intergenic |
| CTTCACTGATAGAGAGGAAG+TGG | 0.479915 | 5:-81889399 | None:intergenic |
| GTCCATTGTTTCAGTTGGCA+AGG | 0.483309 | 5:-81888666 | None:intergenic |
| TGACAACATCTGAACGTCTT+CGG | 0.483647 | 5:-81888819 | None:intergenic |
| GGAAAACATAGCACTGAAGT+AGG | 0.485132 | 5:-81889485 | None:intergenic |
| CAGCAGGTACAGTGTTCAAA+AGG | 0.485488 | 5:+81889518 | MsG0580028529.01.T01:CDS |
| TTGAAGAAGAGATTGAAAGA+TGG | 0.488391 | 5:+81888852 | MsG0580028529.01.T01:CDS |
| ATCACAAAGTTTCCGTTCAG+TGG | 0.495687 | 5:-81888912 | None:intergenic |
| CTGAACGGAAACTTTGTGAT+CGG | 0.497291 | 5:+81888915 | MsG0580028529.01.T01:CDS |
| TTGTCAGTCACGGATGGTGC+TGG | 0.503030 | 5:+81889307 | MsG0580028529.01.T01:CDS |
| TGTACCTGCTGTAATTCATC+AGG | 0.503183 | 5:-81889506 | None:intergenic |
| ATCTAAAACAACATCTATAG+AGG | 0.504654 | 5:-81888405 | None:intergenic |
| TTTCATCTTCTAAACTAGTA+GGG | 0.514168 | 5:-81888436 | None:intergenic |
| GCAAAGTTGATGGTTAGTGC+AGG | 0.515007 | 5:+81888704 | MsG0580028529.01.T01:CDS |
| TCAACACGTAATGGGTAATC+GGG | 0.521709 | 5:-81889268 | None:intergenic |
| CATTCATATTCATCACAGTT+GGG | 0.527750 | 5:+81888479 | MsG0580028529.01.T01:CDS |
| ATGCCGTCCATTGTTTCAGT+TGG | 0.529072 | 5:-81888671 | None:intergenic |
| TCCTCTCTATCAGTGAAGAT+GGG | 0.529089 | 5:+81889404 | MsG0580028529.01.T01:CDS |
| CCATTCATATTCATCACAGT+TGG | 0.531008 | 5:+81888478 | MsG0580028529.01.T01:CDS |
| ATCCTTCACGGATGTTTGCA+GGG | 0.539926 | 5:+81888970 | MsG0580028529.01.T01:CDS |
| GAAGAGATTGGGGAAGCGAT+CGG | 0.540100 | 5:+81889170 | MsG0580028529.01.T01:CDS |
| GAAGGAATATCCCAAAATTG+AGG | 0.542073 | 5:+81889364 | MsG0580028529.01.T01:CDS |
| TAAACAACCGCTCAGGTGAT+CGG | 0.543038 | 5:-81889041 | None:intergenic |
| CAAAGTTGATGGTTAGTGCA+GGG | 0.555798 | 5:+81888705 | MsG0580028529.01.T01:CDS |
| CCAACTGTGATGAATATGAA+TGG | 0.559988 | 5:-81888478 | None:intergenic |
| AGATATTATTTCCCACTGAA+CGG | 0.563648 | 5:+81888900 | MsG0580028529.01.T01:CDS |
| CAACAATTTGGAAGAGATTG+GGG | 0.564161 | 5:+81889160 | MsG0580028529.01.T01:CDS |
| AGGCTTTGATCATGAAGTCT+GGG | 0.565466 | 5:-81888646 | None:intergenic |
| TCACAAAGTTTCCGTTCAGT+GGG | 0.570711 | 5:-81888911 | None:intergenic |
| CGTTCAGATGTTGTCATGGA+AGG | 0.571479 | 5:+81888826 | MsG0580028529.01.T01:CDS |
| GGTTAGTGCAGGGTTTGAGA+AGG | 0.577226 | 5:+81888715 | MsG0580028529.01.T01:CDS |
| ACCCATCTTCACTGATAGAG+AGG | 0.584934 | 5:-81889405 | None:intergenic |
| GAAAACATAGCACTGAAGTA+GGG | 0.592856 | 5:-81889484 | None:intergenic |
| TAAATCCAGAATCATCGCTA+AGG | 0.600639 | 5:-81888613 | None:intergenic |
| TGCTGATTGTCAGTCACGGA+TGG | 0.600899 | 5:+81889301 | MsG0580028529.01.T01:CDS |
| ATGACTCTAAACAACCGCTC+AGG | 0.601313 | 5:-81889048 | None:intergenic |
| TTTGCCGATGCTATTGCTAG+TGG | 0.607076 | 5:+81889015 | MsG0580028529.01.T01:CDS |
| TTCCCTGCAAACATCCGTGA+AGG | 0.612227 | 5:-81888972 | None:intergenic |
| TCATGTTAATGAGTTTGTCA+CGG | 0.613526 | 5:+81888240 | None:intergenic |
| CAAAATCAGCAGCTGAAGTG+CGG | 0.618138 | 5:-81888996 | None:intergenic |
| GTGACTGACAATCAGCATGA+AGG | 0.619865 | 5:-81889295 | None:intergenic |
| AAGACGTTCAGATGTTGTCA+TGG | 0.622352 | 5:+81888822 | MsG0580028529.01.T01:CDS |
| TTCATGCTGATTGTCAGTCA+CGG | 0.623312 | 5:+81889297 | MsG0580028529.01.T01:CDS |
| GAAGGATAAATCAGCAGTGG+AGG | 0.628489 | 5:-81888954 | None:intergenic |
| CGTGTATATCAGTTGCAGGA+GGG | 0.629912 | 5:+81888748 | MsG0580028529.01.T01:CDS |
| CGTGAAGGATAAATCAGCAG+TGG | 0.632684 | 5:-81888957 | None:intergenic |
| TCAGTCACGGATGGTGCTGG+AGG | 0.634051 | 5:+81889310 | MsG0580028529.01.T01:CDS |
| ACAACTTAGTGATTATGTGA+AGG | 0.636749 | 5:+81888532 | MsG0580028529.01.T01:CDS |
| TGATATTGGTAGTAGTATGG+TGG | 0.641647 | 5:+81888508 | MsG0580028529.01.T01:CDS |
| GCTTCCACTAGCAATAGCAT+CGG | 0.641991 | 5:-81889019 | None:intergenic |
| GAGATTGGGGAAGCGATCGG+AGG | 0.645944 | 5:+81889173 | MsG0580028529.01.T01:CDS |
| ACGTGTATATCAGTTGCAGG+AGG | 0.648030 | 5:+81888747 | MsG0580028529.01.T01:CDS |
| GATTCGCCAAGATCCAGCAA+AGG | 0.649881 | 5:+81889220 | MsG0580028529.01.T01:CDS |
| TTGTCACGGCACACTCAATG+TGG | 0.666823 | 5:+81888254 | MsG0580028529.01.T01:CDS |
| TTGCCAACTGAAACAATGGA+CGG | 0.672416 | 5:+81888668 | MsG0580028529.01.T01:CDS |
| TAATATCTTCAAAGCCACGT+TGG | 0.673863 | 5:-81888885 | None:intergenic |
| AATCGGGTGAAGTCCAACAC+CGG | 0.686811 | 5:-81889252 | None:intergenic |
| ATGGATTAAAATTTCCAACG+TGG | 0.715635 | 5:+81888871 | MsG0580028529.01.T01:CDS |
| GTGGAAGCCGATCACCTGAG+CGG | 0.733440 | 5:+81889034 | MsG0580028529.01.T01:CDS |
| AGCCAAATCCAAAATCTACG+AGG | 0.755525 | 5:+81889460 | MsG0580028529.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGCAGTTATTCATTGT+TGG | + | Chr5:81888338-81888357 | MsG0580028529.01.T01:CDS | 25.0% |
| ! | CAAGAAAATCCAAATCTTAT+CGG | + | Chr5:81888563-81888582 | MsG0580028529.01.T01:CDS | 25.0% |
| ! | TAGAAAGCTTATCAAGATTA+GGG | + | Chr5:81888780-81888799 | MsG0580028529.01.T01:CDS | 25.0% |
| ! | TTTCATCTTCTAAACTAGTA+GGG | - | Chr5:81888439-81888458 | None:intergenic | 25.0% |
| !! | AAGAGTTTGAAGAAGATTTT+GGG | + | Chr5:81889333-81889352 | MsG0580028529.01.T01:CDS | 25.0% |
| !! | ATCTAAAACAACATCTATAG+AGG | - | Chr5:81888408-81888427 | None:intergenic | 25.0% |
| !!! | GATCTTCTTTACTTTTTTCT+TGG | + | Chr5:81888287-81888306 | MsG0580028529.01.T01:CDS | 25.0% |
| AACAACAATTTGGAAGAGAT+TGG | + | Chr5:81889158-81889177 | MsG0580028529.01.T01:CDS | 30.0% | |
| ACAACAAAGCAACAACAATT+TGG | + | Chr5:81889148-81889167 | MsG0580028529.01.T01:CDS | 30.0% | |
| ACAACAATTTGGAAGAGATT+GGG | + | Chr5:81889159-81889178 | MsG0580028529.01.T01:CDS | 30.0% | |
| ACAACTTAGTGATTATGTGA+AGG | + | Chr5:81888532-81888551 | MsG0580028529.01.T01:CDS | 30.0% | |
| AGATATTATTTCCCACTGAA+CGG | + | Chr5:81888900-81888919 | MsG0580028529.01.T01:CDS | 30.0% | |
| CATTCATATTCATCACAGTT+GGG | + | Chr5:81888479-81888498 | MsG0580028529.01.T01:CDS | 30.0% | |
| CTTTCATCTTCTAAACTAGT+AGG | - | Chr5:81888440-81888459 | None:intergenic | 30.0% | |
| GTAGAAAGCTTATCAAGATT+AGG | + | Chr5:81888779-81888798 | MsG0580028529.01.T01:CDS | 30.0% | |
| TTGAAGAAGAGATTGAAAGA+TGG | + | Chr5:81888852-81888871 | MsG0580028529.01.T01:CDS | 30.0% | |
| ! | AAATCTTATCGGAATGCTTT+TGG | + | Chr5:81888574-81888593 | MsG0580028529.01.T01:CDS | 30.0% |
| ! | ATGGATTAAAATTTCCAACG+TGG | + | Chr5:81888871-81888890 | MsG0580028529.01.T01:CDS | 30.0% |
| ! | GAAGAGTTTGAAGAAGATTT+TGG | + | Chr5:81889332-81889351 | MsG0580028529.01.T01:CDS | 30.0% |
| ! | TACTGATATTGGTAGTAGTA+TGG | + | Chr5:81888505-81888524 | MsG0580028529.01.T01:CDS | 30.0% |
| !! | AAAAGCATTCCGATAAGATT+TGG | - | Chr5:81888575-81888594 | None:intergenic | 30.0% |
| !!! | ATTTTGGGATATTCCTTCAA+CGG | - | Chr5:81889362-81889381 | None:intergenic | 30.0% |
| !!! | TTTGTGATCGGGTTTTATTT+GGG | + | Chr5:81888927-81888946 | MsG0580028529.01.T01:CDS | 30.0% |
| AAGGTAGTTCAACACGTAAT+GGG | - | Chr5:81889279-81889298 | None:intergenic | 35.0% | |
| CAACAATTTGGAAGAGATTG+GGG | + | Chr5:81889160-81889179 | MsG0580028529.01.T01:CDS | 35.0% | |
| CCAACTGTGATGAATATGAA+TGG | - | Chr5:81888481-81888500 | None:intergenic | 35.0% | |
| CCATTCATATTCATCACAGT+TGG | + | Chr5:81888478-81888497 | MsG0580028529.01.T01:CDS | 35.0% | |
| GAAGGAATATCCCAAAATTG+AGG | + | Chr5:81889364-81889383 | MsG0580028529.01.T01:CDS | 35.0% | |
| TAAATCCAGAATCATCGCTA+AGG | - | Chr5:81888616-81888635 | None:intergenic | 35.0% | |
| TAATATCTTCAAAGCCACGT+TGG | - | Chr5:81888888-81888907 | None:intergenic | 35.0% | |
| TCACAAAACAGCAAAGTTGA+TGG | + | Chr5:81888694-81888713 | MsG0580028529.01.T01:CDS | 35.0% | |
| ! | ACTGTATTGATCACGAAACA+TGG | - | Chr5:81889118-81889137 | None:intergenic | 35.0% |
| ! | ATGCACTCTATCCTCAATTT+TGG | - | Chr5:81889378-81889397 | None:intergenic | 35.0% |
| ! | GAAAACATAGCACTGAAGTA+GGG | - | Chr5:81889487-81889506 | None:intergenic | 35.0% |
| ! | TGATATTGGTAGTAGTATGG+TGG | + | Chr5:81888508-81888527 | MsG0580028529.01.T01:CDS | 35.0% |
| ! | TGCACTCTATCCTCAATTTT+GGG | - | Chr5:81889377-81889396 | None:intergenic | 35.0% |
| ! | TTTTCCTGATGAATTACAGC+AGG | + | Chr5:81889502-81889521 | MsG0580028529.01.T01:CDS | 35.0% |
| !! | TTCAACACGTAATGGGTAAT+CGG | - | Chr5:81889272-81889291 | None:intergenic | 35.0% |
| !!! | CTTTGTGATCGGGTTTTATT+TGG | + | Chr5:81888926-81888945 | MsG0580028529.01.T01:CDS | 35.0% |
| !!! | GAAGCTATTAGAGAGCATTT+TGG | + | Chr5:81889436-81889455 | MsG0580028529.01.T01:CDS | 35.0% |
| AAGACGTTCAGATGTTGTCA+TGG | + | Chr5:81888822-81888841 | MsG0580028529.01.T01:CDS | 40.0% | |
| AGCCAAATCCAAAATCTACG+AGG | + | Chr5:81889460-81889479 | MsG0580028529.01.T01:CDS | 40.0% | |
| ATCACAAAGTTTCCGTTCAG+TGG | - | Chr5:81888915-81888934 | None:intergenic | 40.0% | |
| CAGTTATTCATTGTTGGAGC+AGG | + | Chr5:81888344-81888363 | MsG0580028529.01.T01:CDS | 40.0% | |
| CTGAACGGAAACTTTGTGAT+CGG | + | Chr5:81888915-81888934 | MsG0580028529.01.T01:CDS | 40.0% | |
| GAAGGTAGTTCAACACGTAA+TGG | - | Chr5:81889280-81889299 | None:intergenic | 40.0% | |
| GAATACCTTAGCGATGATTC+TGG | + | Chr5:81888608-81888627 | MsG0580028529.01.T01:CDS | 40.0% | |
| TCACAAAGTTTCCGTTCAGT+GGG | - | Chr5:81888914-81888933 | None:intergenic | 40.0% | |
| TCCTCTCTATCAGTGAAGAT+GGG | + | Chr5:81889404-81889423 | MsG0580028529.01.T01:CDS | 40.0% | |
| TGAACGGAAACTTTGTGATC+GGG | + | Chr5:81888916-81888935 | MsG0580028529.01.T01:CDS | 40.0% | |
| TGACAACATCTGAACGTCTT+CGG | - | Chr5:81888822-81888841 | None:intergenic | 40.0% | |
| TGTACCTGCTGTAATTCATC+AGG | - | Chr5:81889509-81889528 | None:intergenic | 40.0% | |
| TTCCTCTCTATCAGTGAAGA+TGG | + | Chr5:81889403-81889422 | MsG0580028529.01.T01:CDS | 40.0% | |
| TTGCCAACTGAAACAATGGA+CGG | + | Chr5:81888668-81888687 | MsG0580028529.01.T01:CDS | 40.0% | |
| ! | AAGGCTTTGATCATGAAGTC+TGG | - | Chr5:81888650-81888669 | None:intergenic | 40.0% |
| ! | AGGCTTTGATCATGAAGTCT+GGG | - | Chr5:81888649-81888668 | None:intergenic | 40.0% |
| ! | GGAAAACATAGCACTGAAGT+AGG | - | Chr5:81889488-81889507 | None:intergenic | 40.0% |
| ! | GTCCTCGTAGATTTTGGATT+TGG | - | Chr5:81889465-81889484 | None:intergenic | 40.0% |
| ! | TTCATGCTGATTGTCAGTCA+CGG | + | Chr5:81889297-81889316 | MsG0580028529.01.T01:CDS | 40.0% |
| !! | AGATTTTGGGTATCCGTTGA+AGG | + | Chr5:81889346-81889365 | MsG0580028529.01.T01:CDS | 40.0% |
| !! | CAAAGTTGATGGTTAGTGCA+GGG | + | Chr5:81888705-81888724 | MsG0580028529.01.T01:CDS | 40.0% |
| !! | CACTGCTGATTTATCCTTCA+CGG | + | Chr5:81888958-81888977 | MsG0580028529.01.T01:CDS | 40.0% |
| !! | TCAACACGTAATGGGTAATC+GGG | - | Chr5:81889271-81889290 | None:intergenic | 40.0% |
| ACCCATCTTCACTGATAGAG+AGG | - | Chr5:81889408-81889427 | None:intergenic | 45.0% | |
| ACGTGTATATCAGTTGCAGG+AGG | + | Chr5:81888747-81888766 | MsG0580028529.01.T01:CDS | 45.0% | |
| AGCCTTGCCAACTGAAACAA+TGG | + | Chr5:81888664-81888683 | MsG0580028529.01.T01:CDS | 45.0% | |
| ATCCTTCACGGATGTTTGCA+GGG | + | Chr5:81888970-81888989 | MsG0580028529.01.T01:CDS | 45.0% | |
| ATGACTCTAAACAACCGCTC+AGG | - | Chr5:81889051-81889070 | None:intergenic | 45.0% | |
| ATGCCGTCCATTGTTTCAGT+TGG | - | Chr5:81888674-81888693 | None:intergenic | 45.0% | |
| CAAAATCAGCAGCTGAAGTG+CGG | - | Chr5:81888999-81889018 | None:intergenic | 45.0% | |
| CAGCAGGTACAGTGTTCAAA+AGG | + | Chr5:81889518-81889537 | MsG0580028529.01.T01:CDS | 45.0% | |
| CAGTTGCAGGAGGGAATATT+TGG | + | Chr5:81888757-81888776 | MsG0580028529.01.T01:CDS | 45.0% | |
| CGAAACATGGACTTGAACTC+TGG | - | Chr5:81889105-81889124 | None:intergenic | 45.0% | |
| CGTGAAGGATAAATCAGCAG+TGG | - | Chr5:81888960-81888979 | None:intergenic | 45.0% | |
| CGTGTATATCAGTTGCAGGA+GGG | + | Chr5:81888748-81888767 | MsG0580028529.01.T01:CDS | 45.0% | |
| CGTTCAGATGTTGTCATGGA+AGG | + | Chr5:81888826-81888845 | MsG0580028529.01.T01:CDS | 45.0% | |
| CTTCACTGATAGAGAGGAAG+TGG | - | Chr5:81889402-81889421 | None:intergenic | 45.0% | |
| GAAGGATAAATCAGCAGTGG+AGG | - | Chr5:81888957-81888976 | None:intergenic | 45.0% | |
| GCTTCCACTAGCAATAGCAT+CGG | - | Chr5:81889022-81889041 | None:intergenic | 45.0% | |
| GTCCATTGTTTCAGTTGGCA+AGG | - | Chr5:81888669-81888688 | None:intergenic | 45.0% | |
| GTGACTGACAATCAGCATGA+AGG | - | Chr5:81889298-81889317 | None:intergenic | 45.0% | |
| TAAACAACCGCTCAGGTGAT+CGG | - | Chr5:81889044-81889063 | None:intergenic | 45.0% | |
| TATCCTTCACGGATGTTTGC+AGG | + | Chr5:81888969-81888988 | MsG0580028529.01.T01:CDS | 45.0% | |
| TCGACGTGTATATCAGTTGC+AGG | + | Chr5:81888744-81888763 | MsG0580028529.01.T01:CDS | 45.0% | |
| TTTGCCGATGCTATTGCTAG+TGG | + | Chr5:81889015-81889034 | MsG0580028529.01.T01:CDS | 45.0% | |
| ! | AGTAGGGTCCTCGTAGATTT+TGG | - | Chr5:81889471-81889490 | None:intergenic | 45.0% |
| !! | CAGTTGGGCAGTACTGATAT+TGG | + | Chr5:81888494-81888513 | MsG0580028529.01.T01:CDS | 45.0% |
| !! | GCAAAGTTGATGGTTAGTGC+AGG | + | Chr5:81888704-81888723 | MsG0580028529.01.T01:CDS | 45.0% |
| AATCGGGTGAAGTCCAACAC+CGG | - | Chr5:81889255-81889274 | None:intergenic | 50.0% | |
| GAAGAGATTGGGGAAGCGAT+CGG | + | Chr5:81889170-81889189 | MsG0580028529.01.T01:CDS | 50.0% | |
| GATTCGCCAAGATCCAGCAA+AGG | + | Chr5:81889220-81889239 | MsG0580028529.01.T01:CDS | 50.0% | |
| GGTTAGTGCAGGGTTTGAGA+AGG | + | Chr5:81888715-81888734 | MsG0580028529.01.T01:CDS | 50.0% | |
| TTCCCTGCAAACATCCGTGA+AGG | - | Chr5:81888975-81888994 | None:intergenic | 50.0% | |
| TTGTCACGGCACACTCAATG+TGG | + | Chr5:81888254-81888273 | MsG0580028529.01.T01:CDS | 50.0% | |
| ! | TGCTGATTGTCAGTCACGGA+TGG | + | Chr5:81889301-81889320 | MsG0580028529.01.T01:CDS | 50.0% |
| !!! | AGCGATCGGAGGCATTTTCA+TGG | + | Chr5:81889184-81889203 | MsG0580028529.01.T01:CDS | 50.0% |
| !!! | CGGAGGCATTTTCATGGAGT+TGG | + | Chr5:81889190-81889209 | MsG0580028529.01.T01:CDS | 50.0% |
| AAGGCAGCAGTTTCCGGTGT+TGG | + | Chr5:81889239-81889258 | MsG0580028529.01.T01:CDS | 55.0% | |
| CCAGCAAAGGCAGCAGTTTC+CGG | + | Chr5:81889233-81889252 | MsG0580028529.01.T01:CDS | 55.0% | |
| CTGCTGCCTTTGCTGGATCT+TGG | - | Chr5:81889229-81889248 | None:intergenic | 55.0% | |
| TTGTCAGTCACGGATGGTGC+TGG | + | Chr5:81889307-81889326 | MsG0580028529.01.T01:CDS | 55.0% | |
| CCGGAAACTGCTGCCTTTGC+TGG | - | Chr5:81889236-81889255 | None:intergenic | 60.0% | |
| GAGATTGGGGAAGCGATCGG+AGG | + | Chr5:81889173-81889192 | MsG0580028529.01.T01:CDS | 60.0% | |
| GTGGAAGCCGATCACCTGAG+CGG | + | Chr5:81889034-81889053 | MsG0580028529.01.T01:CDS | 60.0% | |
| ! | TCAGTCACGGATGGTGCTGG+AGG | + | Chr5:81889310-81889329 | MsG0580028529.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 81888248 | 81889544 | 81888248 | ID=MsG0580028529.01;Name=MsG0580028529.01 |
| Chr5 | mRNA | 81888248 | 81889544 | 81888248 | ID=MsG0580028529.01.T01;Parent=MsG0580028529.01;Name=MsG0580028529.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|2|0|403 |
| Chr5 | exon | 81888248 | 81889055 | 81888248 | ID=MsG0580028529.01.T01:exon:21076;Parent=MsG0580028529.01.T01 |
| Chr5 | exon | 81889141 | 81889544 | 81889141 | ID=MsG0580028529.01.T01:exon:21077;Parent=MsG0580028529.01.T01 |
| Chr5 | CDS | 81888248 | 81889055 | 81888248 | ID=MsG0580028529.01.T01:cds;Parent=MsG0580028529.01.T01 |
| Chr5 | CDS | 81889141 | 81889544 | 81889141 | ID=MsG0580028529.01.T01:cds;Parent=MsG0580028529.01.T01 |
| Gene Sequence |
| Protein sequence |
