Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030171.01.T01 | XP_003618047.1 | 98.091 | 419 | 8 | 0 | 2 | 420 | 3 | 421 | 0 | 837 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030171.01.T01 | Q9SU13 | 58.647 | 399 | 137 | 5 | 1 | 396 | 4 | 377 | 1.83E-157 | 453 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030171.01.T01 | G7K0M1 | 98.091 | 419 | 8 | 0 | 2 | 420 | 3 | 421 | 0.0 | 837 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380012697.01 | MsG0580030171.01 | 0.866582 | 2.566263e-65 | 8.693524e-62 |
| MsG0480020448.01 | MsG0580030171.01 | 0.806904 | 6.558613e-50 | 3.602635e-47 |
| MsG0480021753.01 | MsG0580030171.01 | 0.808477 | 3.038056e-50 | 1.738919e-47 |
| MsG0480023279.01 | MsG0580030171.01 | 0.851112 | 1.097088e-60 | 2.197056e-57 |
| MsG0480023418.01 | MsG0580030171.01 | 0.847209 | 1.336523e-59 | 2.358071e-56 |
| MsG0580025071.01 | MsG0580030171.01 | 0.834650 | 2.651750e-56 | 3.166744e-53 |
| MsG0380015858.01 | MsG0580030171.01 | 0.805453 | 1.325283e-49 | 7.014232e-47 |
| MsG0380016141.01 | MsG0580030171.01 | 0.818682 | 1.725399e-52 | 1.300213e-49 |
| MsG0380016156.01 | MsG0580030171.01 | 0.839667 | 1.381294e-57 | 1.920896e-54 |
| MsG0380016324.01 | MsG0580030171.01 | 0.824917 | 6.225694e-54 | 5.591384e-51 |
| MsG0380016339.01 | MsG0580030171.01 | 0.833467 | 5.244754e-56 | 6.045040e-53 |
| MsG0380016567.01 | MsG0580030171.01 | 0.818262 | 2.148983e-52 | 1.600689e-49 |
| MsG0380017212.01 | MsG0580030171.01 | 0.815855 | 7.465126e-52 | 5.205687e-49 |
| MsG0380017435.01 | MsG0580030171.01 | 0.832496 | 9.145140e-56 | 1.023953e-52 |
| MsG0380017660.01 | MsG0580030171.01 | 0.825497 | 4.538578e-54 | 4.143729e-51 |
| MsG0580030171.01 | MsG0780036793.01 | 0.828728 | 7.651880e-55 | 7.669316e-52 |
| MsG0580030171.01 | MsG0880042849.01 | 0.844830 | 5.929173e-59 | 9.691183e-56 |
| MsG0580030171.01 | MsG0880043058.01 | 0.866344 | 3.054145e-65 | 1.025988e-61 |
| MsG0580030171.01 | MsG0880047049.01 | 0.818511 | 1.886870e-52 | 1.415244e-49 |
| MsG0280007598.01 | MsG0580030171.01 | 0.862370 | 5.316432e-64 | 1.553377e-60 |
| MsG0280007923.01 | MsG0580030171.01 | 0.866174 | 3.457740e-65 | 1.154551e-61 |
| MsG0280009108.01 | MsG0580030171.01 | 0.813434 | 2.564598e-51 | 1.674638e-48 |
| MsG0280009139.01 | MsG0580030171.01 | 0.819173 | 1.334279e-52 | 1.019221e-49 |
| MsG0280010268.01 | MsG0580030171.01 | 0.800978 | 1.118235e-48 | 5.278990e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030171.01.T01 | MTR_5g098420 | 98.091 | 419 | 8 | 0 | 2 | 420 | 3 | 421 | 0.0 | 837 |
| MsG0580030171.01.T01 | MTR_3g460780 | 65.372 | 309 | 105 | 2 | 33 | 339 | 30 | 338 | 3.80e-135 | 394 |
| MsG0580030171.01.T01 | MTR_7g075453 | 39.423 | 416 | 215 | 10 | 27 | 420 | 16 | 416 | 3.93e-81 | 256 |
| MsG0580030171.01.T01 | MTR_8g069000 | 90.164 | 61 | 6 | 0 | 1 | 61 | 1 | 61 | 1.08e-30 | 113 |
| MsG0580030171.01.T01 | MTR_1g009950 | 36.813 | 182 | 104 | 4 | 18 | 190 | 7 | 186 | 3.02e-27 | 109 |
| MsG0580030171.01.T01 | MTR_2g437700 | 27.378 | 347 | 232 | 11 | 8 | 337 | 1 | 344 | 1.32e-23 | 102 |
| MsG0580030171.01.T01 | MTR_1g009940 | 35.374 | 147 | 92 | 2 | 34 | 179 | 25 | 169 | 3.39e-22 | 96.3 |
| MsG0580030171.01.T01 | MTR_1g009910 | 34.694 | 147 | 93 | 2 | 34 | 179 | 25 | 169 | 1.50e-21 | 94.4 |
| MsG0580030171.01.T01 | MTR_3g108760 | 36.129 | 155 | 92 | 4 | 34 | 187 | 25 | 173 | 2.76e-19 | 87.4 |
| MsG0580030171.01.T01 | MTR_1g009920 | 35.345 | 116 | 73 | 1 | 34 | 149 | 25 | 138 | 1.20e-18 | 84.7 |
| MsG0580030171.01.T01 | MTR_2g017970 | 30.603 | 232 | 123 | 8 | 195 | 416 | 68 | 271 | 5.82e-17 | 81.3 |
| MsG0580030171.01.T01 | MTR_2g090765 | 31.984 | 247 | 136 | 9 | 183 | 419 | 25 | 249 | 9.36e-17 | 79.7 |
| MsG0580030171.01.T01 | MTR_4g059840 | 31.553 | 206 | 126 | 7 | 176 | 374 | 56 | 253 | 1.22e-16 | 80.5 |
| MsG0580030171.01.T01 | MTR_4g059790 | 31.373 | 204 | 125 | 7 | 178 | 374 | 58 | 253 | 1.53e-16 | 80.1 |
| MsG0580030171.01.T01 | MTR_2g017960 | 29.237 | 236 | 129 | 8 | 195 | 420 | 68 | 275 | 6.25e-15 | 75.1 |
| MsG0580030171.01.T01 | MTR_4g053380 | 32.124 | 193 | 114 | 7 | 185 | 363 | 63 | 252 | 1.25e-14 | 74.3 |
| MsG0580030171.01.T01 | MTR_4g059720 | 29.193 | 161 | 105 | 3 | 184 | 337 | 31 | 189 | 1.74e-13 | 70.5 |
| MsG0580030171.01.T01 | MTR_8g087460 | 27.969 | 261 | 153 | 10 | 178 | 420 | 73 | 316 | 6.37e-12 | 66.6 |
| MsG0580030171.01.T01 | MTR_8g087470 | 28.016 | 257 | 146 | 9 | 187 | 420 | 76 | 316 | 1.43e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580030171.01.T01 | AT4G12730 | 58.647 | 399 | 137 | 5 | 1 | 396 | 4 | 377 | 1.86e-158 | 453 |
| MsG0580030171.01.T01 | AT5G55730 | 56.906 | 362 | 140 | 4 | 33 | 379 | 25 | 385 | 1.16e-131 | 385 |
| MsG0580030171.01.T01 | AT5G55730 | 56.906 | 362 | 140 | 4 | 33 | 379 | 25 | 385 | 1.16e-131 | 385 |
| MsG0580030171.01.T01 | AT2G45470 | 43.112 | 392 | 201 | 8 | 16 | 391 | 9 | 394 | 2.82e-90 | 280 |
| MsG0580030171.01.T01 | AT3G60900 | 41.422 | 408 | 201 | 10 | 8 | 391 | 7 | 400 | 7.93e-87 | 271 |
| MsG0580030171.01.T01 | AT3G12660 | 43.421 | 152 | 83 | 2 | 29 | 179 | 20 | 169 | 4.38e-31 | 120 |
| MsG0580030171.01.T01 | AT3G46550 | 29.480 | 346 | 208 | 12 | 34 | 350 | 30 | 368 | 1.96e-27 | 113 |
| MsG0580030171.01.T01 | AT2G24450 | 37.427 | 171 | 96 | 5 | 10 | 179 | 9 | 169 | 6.30e-25 | 103 |
| MsG0580030171.01.T01 | AT4G31370 | 35.135 | 148 | 92 | 3 | 33 | 179 | 25 | 169 | 3.22e-21 | 93.2 |
| MsG0580030171.01.T01 | AT5G03170 | 29.474 | 190 | 121 | 6 | 179 | 360 | 19 | 203 | 4.67e-17 | 80.9 |
| MsG0580030171.01.T01 | AT2G20520 | 34.634 | 205 | 117 | 7 | 180 | 374 | 23 | 220 | 4.36e-16 | 77.8 |
| MsG0580030171.01.T01 | AT1G03870 | 31.016 | 187 | 112 | 7 | 194 | 367 | 38 | 220 | 1.30e-12 | 67.8 |
| MsG0580030171.01.T01 | AT5G60490 | 31.034 | 116 | 74 | 3 | 226 | 337 | 74 | 187 | 2.07e-11 | 64.3 |
Find 124 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTACTCTTTGCTGCTTT+TGG | 0.147168 | 5:+107730250 | None:intergenic |
| GGTGATTTGGTGGAGTTTCT+TGG | 0.245142 | 5:+107730990 | None:intergenic |
| GATGCCAAGAGAATTGTTTA+AGG | 0.254991 | 5:-107730311 | MsG0580030171.01.T01:CDS |
| CAGTTTGTTTATTGGTGCTT+TGG | 0.270880 | 5:-107730071 | MsG0580030171.01.T01:CDS |
| GATGATGAAAATGGAGTTAA+TGG | 0.275353 | 5:-107730130 | MsG0580030171.01.T01:CDS |
| GTCAATATCACAAACCTAAA+AGG | 0.277047 | 5:-107730901 | MsG0580030171.01.T01:CDS |
| AGAATTGTTTAAGGGAGTTA+AGG | 0.283558 | 5:-107730302 | MsG0580030171.01.T01:CDS |
| TCTGCGTCGGCGGAGCTTAA+AGG | 0.293364 | 5:+107730772 | None:intergenic |
| ATCTGTAATGATTGGTATAC+TGG | 0.301366 | 5:+107730514 | None:intergenic |
| CTGGAACACCGTGGTATAAT+AGG | 0.314783 | 5:+107730533 | None:intergenic |
| TCAAGGGTTGAGATTCATTA+TGG | 0.318794 | 5:-107730101 | MsG0580030171.01.T01:CDS |
| CCAAGTGACATTGATTTAAT+CGG | 0.340784 | 5:-107730733 | MsG0580030171.01.T01:CDS |
| AAAGGTGGCAAAGTCGGTTT+TGG | 0.345872 | 5:-107730883 | MsG0580030171.01.T01:CDS |
| ATCATATTTGTTATGGCCTT+CGG | 0.360900 | 5:+107730453 | None:intergenic |
| CAAGAAGAAGAAGGCGGCGC+CGG | 0.364739 | 5:-107730227 | MsG0580030171.01.T01:CDS |
| CAGTGTTAACTTTGGTTTCA+AGG | 0.373237 | 5:+107730398 | None:intergenic |
| ATTTCAACATCTGTAATGAT+TGG | 0.375565 | 5:+107730506 | None:intergenic |
| GCGCGTGATGTTGTGGTTGC+CGG | 0.375636 | 5:+107731215 | None:intergenic |
| GTCGATGGCTAGGATGGTTA+TGG | 0.377750 | 5:+107731107 | None:intergenic |
| GGCGGCGCCGGCGGCGGATG+AGG | 0.389395 | 5:-107730215 | MsG0580030171.01.T01:CDS |
| GGAGGTGGCGGCAGCGGCGA+TGG | 0.390080 | 5:+107731236 | None:intergenic |
| AAAGCTGCTGATGATGAAAA+TGG | 0.396296 | 5:-107730139 | MsG0580030171.01.T01:CDS |
| ATGAAGACCGTCGTTGTCTT+CGG | 0.397307 | 5:+107730858 | None:intergenic |
| GGCGGTTCCAGCGGCGGATC+CGG | 0.405430 | 5:+107730930 | None:intergenic |
| CTGTGAAATCATATTTGTTA+TGG | 0.408434 | 5:+107730446 | None:intergenic |
| ATCGCCGCTGCCGCCACCTC+CGG | 0.408440 | 5:-107731234 | MsG0580030171.01.T01:CDS |
| TTAAAGGATTACTGATTTGA+AGG | 0.408846 | 5:+107730788 | None:intergenic |
| GAAGAAGGCGGCGCCGGCGG+CGG | 0.414846 | 5:-107730221 | MsG0580030171.01.T01:CDS |
| GTTAACACTGCAAATATTGT+TGG | 0.417708 | 5:-107730385 | MsG0580030171.01.T01:CDS |
| GAAGAAGAAGGCGGCGCCGG+CGG | 0.417754 | 5:-107730224 | MsG0580030171.01.T01:CDS |
| GGAGTTAATGGATTAAATCA+AGG | 0.421979 | 5:-107730118 | MsG0580030171.01.T01:CDS |
| ATGCCAAGAGAATTGTTTAA+GGG | 0.423949 | 5:-107730310 | MsG0580030171.01.T01:CDS |
| TGTAGTGTATAAGATAAGTA+AGG | 0.425772 | 5:-107730338 | MsG0580030171.01.T01:CDS |
| ATTGTTTAAGGGAGTTAAGG+AGG | 0.430839 | 5:-107730299 | MsG0580030171.01.T01:CDS |
| TCAGAATCTGCATCTGCATC+TGG | 0.432705 | 5:+107730169 | None:intergenic |
| CTTATCTTATACACTACAAA+AGG | 0.441117 | 5:+107730343 | None:intergenic |
| GTTTGTGATATTGACGTAAC+CGG | 0.442226 | 5:+107730909 | None:intergenic |
| CGCCGACGCAGAAGCGCCGA+CGG | 0.447538 | 5:-107730761 | MsG0580030171.01.T01:CDS |
| GTTGCCGGAGGTGGCGGCAG+CGG | 0.450353 | 5:+107731230 | None:intergenic |
| AATATTTGCAGTGTTAACTT+TGG | 0.452437 | 5:+107730390 | None:intergenic |
| GTGTGGTACCGTCGGTGATT+TGG | 0.461418 | 5:+107730977 | None:intergenic |
| TCAACGGATTCTTTGAAAGT+AGG | 0.465548 | 5:+107730649 | None:intergenic |
| ACTCCCTTAAACAATTCTCT+TGG | 0.467873 | 5:+107730307 | None:intergenic |
| TGCCGTCGGCGCTTCTGCGT+CGG | 0.476325 | 5:+107730759 | None:intergenic |
| GGTGGCGGCAGCGGCGATGG+TGG | 0.476691 | 5:+107731239 | None:intergenic |
| GATGGAAAGGTAGTGGTTGA+AGG | 0.482791 | 5:+107731164 | None:intergenic |
| CCGATTAAATCAATGTCACT+TGG | 0.484710 | 5:+107730733 | None:intergenic |
| CAGCGGCGGATCCGGTAGCT+TGG | 0.489876 | 5:+107730938 | None:intergenic |
| GTTGTGGTTGCCGGAGGTGG+CGG | 0.490801 | 5:+107731224 | None:intergenic |
| CAAGCTACCGGATCCGCCGC+TGG | 0.491705 | 5:-107730937 | MsG0580030171.01.T01:CDS |
| GGAGAGGTGTTTGTCTAGGA+GGG | 0.492717 | 5:+107731068 | None:intergenic |
| TCCTCCATGTTCCAAGCTAC+CGG | 0.493888 | 5:-107730949 | MsG0580030171.01.T01:CDS |
| CTCATCGGCGAGGTGGGTGA+TGG | 0.495826 | 5:+107731146 | None:intergenic |
| GTAACAAATCTGCAAAAGAT+TGG | 0.498004 | 5:+107730689 | None:intergenic |
| CCGCTGACGGCGGTATCTGT+TGG | 0.500494 | 5:+107730604 | None:intergenic |
| CAACGGATTCTTTGAAAGTA+GGG | 0.501623 | 5:+107730650 | None:intergenic |
| GATGTTGTGGTTGCCGGAGG+TGG | 0.513269 | 5:+107731221 | None:intergenic |
| AACAGTTAAACCGCCGTCAA+CGG | 0.515932 | 5:+107730633 | None:intergenic |
| TGATGGAGAGGTGTTTGTCT+AGG | 0.520466 | 5:+107731064 | None:intergenic |
| TGGTTGAAGGTGGAGAAGCC+AGG | 0.520492 | 5:+107731177 | None:intergenic |
| AGGATTACTGATTTGAAGGA+CGG | 0.524929 | 5:+107730792 | None:intergenic |
| TCTGCATCCTCATCCGCCGC+CGG | 0.525089 | 5:+107730208 | None:intergenic |
| GGTGGGTGATGGAAAGGTAG+TGG | 0.528798 | 5:+107731157 | None:intergenic |
| GGCATTGTCGATGGCTAGGA+TGG | 0.528895 | 5:+107731101 | None:intergenic |
| TCTGCATCTGGTCCATCTGC+CGG | 0.531869 | 5:+107730181 | None:intergenic |
| GACGTAACCGGCGGTTCCAG+CGG | 0.539035 | 5:+107730921 | None:intergenic |
| TGGAGAGGTGTTTGTCTAGG+AGG | 0.539087 | 5:+107731067 | None:intergenic |
| CGTCGGCGCTTCTGCGTCGG+CGG | 0.540634 | 5:+107730762 | None:intergenic |
| CGGCGAGGTGGGTGATGGAA+AGG | 0.540952 | 5:+107731151 | None:intergenic |
| AACCTAAAAGGTGGCAAAGT+CGG | 0.542701 | 5:-107730889 | MsG0580030171.01.T01:CDS |
| GAGTAAGAGCAAGAAGAAGA+AGG | 0.550005 | 5:-107730236 | MsG0580030171.01.T01:CDS |
| ACTTTCAAAGAATCCGTTGA+CGG | 0.551042 | 5:-107730646 | MsG0580030171.01.T01:CDS |
| GAGTTAATGGATTAAATCAA+GGG | 0.551140 | 5:-107730117 | MsG0580030171.01.T01:CDS |
| GACGTATGGAAGTTCTTCTA+CGG | 0.551640 | 5:+107730819 | None:intergenic |
| TGGCGGCATTGTCGATGGCT+AGG | 0.553261 | 5:+107731097 | None:intergenic |
| GGATCCGCCGCTGGAACCGC+CGG | 0.554442 | 5:-107730928 | MsG0580030171.01.T01:CDS |
| GTTGATCTCATCGGCGAGGT+GGG | 0.557690 | 5:+107731140 | None:intergenic |
| CATGTCTTAGTCGACTACTT+CGG | 0.559079 | 5:-107731015 | MsG0580030171.01.T01:CDS |
| GATGCAGACTCACCGGCAGA+TGG | 0.563432 | 5:-107730193 | MsG0580030171.01.T01:CDS |
| CGACTAAGACATGGAGGGAG+AGG | 0.569245 | 5:+107731025 | None:intergenic |
| AGACATGGCGGCATTGTCGA+TGG | 0.569829 | 5:+107731092 | None:intergenic |
| GGTTGATCTCATCGGCGAGG+TGG | 0.572163 | 5:+107731139 | None:intergenic |
| TGGTACCGTCGGTGATTTGG+TGG | 0.574288 | 5:+107730980 | None:intergenic |
| CTAGAATGCGCGTGATGTTG+TGG | 0.578501 | 5:+107731208 | None:intergenic |
| GGATGAGGATGCAGACTCAC+CGG | 0.583422 | 5:-107730200 | MsG0580030171.01.T01:CDS |
| GATTTCACAGTGCAAAATGA+TGG | 0.584777 | 5:-107730433 | MsG0580030171.01.T01:CDS |
| ATGTCACTTGGAGCTGCCGT+CGG | 0.585801 | 5:+107730745 | None:intergenic |
| GGTTGAAGGTGGAGAAGCCA+GGG | 0.586503 | 5:+107731178 | None:intergenic |
| TGAAGGACGGAGATGACGTA+TGG | 0.589268 | 5:+107730805 | None:intergenic |
| GAACACCGTGGTATAATAGG+AGG | 0.593137 | 5:+107730536 | None:intergenic |
| TTGACGACGGTTGATCTCAT+CGG | 0.593521 | 5:+107731131 | None:intergenic |
| ATCGGAATTATGTCAAAACA+AGG | 0.597188 | 5:-107730715 | MsG0580030171.01.T01:CDS |
| GGAAAGGTAGTGGTTGAAGG+TGG | 0.597732 | 5:+107731167 | None:intergenic |
| GTAACCGGCGGTTCCAGCGG+CGG | 0.607268 | 5:+107730924 | None:intergenic |
| TTCAAAGAATCCGTTGACGG+CGG | 0.608626 | 5:-107730643 | MsG0580030171.01.T01:CDS |
| TTTGTCTAGGAGGGAAGACA+TGG | 0.612461 | 5:+107731077 | None:intergenic |
| AATATCACAAACCTAAAAGG+TGG | 0.621855 | 5:-107730898 | MsG0580030171.01.T01:CDS |
| GTGAGGGAAAATAGAACAAG+TGG | 0.628337 | 5:+107731285 | None:intergenic |
| CGCATTCTAGCTAAACACCC+TGG | 0.633646 | 5:-107731195 | MsG0580030171.01.T01:CDS |
| CGAAGTAGTCGACTAAGACA+TGG | 0.635651 | 5:+107731016 | None:intergenic |
| CCAACAGATACCGCCGTCAG+CGG | 0.650324 | 5:-107730604 | MsG0580030171.01.T01:CDS |
| TGCAGAGAAACCGCTGACGG+CGG | 0.650965 | 5:+107730594 | None:intergenic |
| GGAGACAAGTGTGGTACCGT+CGG | 0.651999 | 5:+107730969 | None:intergenic |
| ATGAACACATTAGCAACCGA+AGG | 0.652043 | 5:-107730469 | MsG0580030171.01.T01:CDS |
| CGTGATGTTGTGGTTGCCGG+AGG | 0.655765 | 5:+107731218 | None:intergenic |
| TGAGTGATAATATGAGTGTG+AGG | 0.656845 | 5:+107731268 | None:intergenic |
| GGATGGTTATGGTTTGACGA+CGG | 0.659324 | 5:+107731118 | None:intergenic |
| GTCTAGGAGGGAAGACATGG+CGG | 0.661578 | 5:+107731080 | None:intergenic |
| AACACCGTGGTATAATAGGA+GGG | 0.667412 | 5:+107730537 | None:intergenic |
| TAAGAGCAAGAAGAAGAAGG+CGG | 0.669561 | 5:-107730233 | MsG0580030171.01.T01:CDS |
| GGATCCGGTAGCTTGGAACA+TGG | 0.672727 | 5:+107730945 | None:intergenic |
| GAGTGATAATATGAGTGTGA+GGG | 0.673705 | 5:+107731269 | None:intergenic |
| CTTTGCAGAGAAACCGCTGA+CGG | 0.676720 | 5:+107730591 | None:intergenic |
| GTTTCCCTCCTATTATACCA+CGG | 0.679718 | 5:-107730541 | MsG0580030171.01.T01:CDS |
| AAACTCCACCAAATCACCGA+CGG | 0.683966 | 5:-107730985 | MsG0580030171.01.T01:CDS |
| GACGGTTGATCTCATCGGCG+AGG | 0.688199 | 5:+107731136 | None:intergenic |
| AGTAGTCGACTAAGACATGG+AGG | 0.689421 | 5:+107731019 | None:intergenic |
| TGTGATATTGACGTAACCGG+CGG | 0.692479 | 5:+107730912 | None:intergenic |
| GAACATGGAGGAGACAAGTG+TGG | 0.699589 | 5:+107730960 | None:intergenic |
| TTTGGTGCCGAAGACAACGA+CGG | 0.700628 | 5:-107730865 | MsG0580030171.01.T01:CDS |
| GTAGTCGACTAAGACATGGA+GGG | 0.706207 | 5:+107731020 | None:intergenic |
| CAGATGTTGAAATCAAACAA+CGG | 0.715747 | 5:-107730496 | MsG0580030171.01.T01:CDS |
| TCCGGTAGCTTGGAACATGG+AGG | 0.720001 | 5:+107730948 | None:intergenic |
| ATTGGTATACTGGAACACCG+TGG | 0.802627 | 5:+107730524 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTATTTTTCAGTTTGTTTAT+TGG | - | Chr5:107731287-107731306 | MsG0580030171.01.T01:CDS | 20.0% |
| ! | AATATTTGCAGTGTTAACTT+TGG | + | Chr5:107730979-107730998 | None:intergenic | 25.0% |
| ! | ATTTCAACATCTGTAATGAT+TGG | + | Chr5:107730863-107730882 | None:intergenic | 25.0% |
| ! | CTTATCTTATACACTACAAA+AGG | + | Chr5:107731026-107731045 | None:intergenic | 25.0% |
| ! | TGTAGTGTATAAGATAAGTA+AGG | - | Chr5:107731028-107731047 | MsG0580030171.01.T01:CDS | 25.0% |
| !! | CTGTGAAATCATATTTGTTA+TGG | + | Chr5:107730923-107730942 | None:intergenic | 25.0% |
| !!! | GAGTTAATGGATTAAATCAA+GGG | - | Chr5:107731249-107731268 | MsG0580030171.01.T01:CDS | 25.0% |
| !!! | TTAAAGGATTACTGATTTGA+AGG | + | Chr5:107730581-107730600 | None:intergenic | 25.0% |
| AATATCACAAACCTAAAAGG+TGG | - | Chr5:107730468-107730487 | MsG0580030171.01.T01:CDS | 30.0% | |
| AGAATTGTTTAAGGGAGTTA+AGG | - | Chr5:107731064-107731083 | MsG0580030171.01.T01:CDS | 30.0% | |
| ATCGGAATTATGTCAAAACA+AGG | - | Chr5:107730651-107730670 | MsG0580030171.01.T01:CDS | 30.0% | |
| ATCTGTAATGATTGGTATAC+TGG | + | Chr5:107730855-107730874 | None:intergenic | 30.0% | |
| ATGCCAAGAGAATTGTTTAA+GGG | - | Chr5:107731056-107731075 | MsG0580030171.01.T01:CDS | 30.0% | |
| CAGATGTTGAAATCAAACAA+CGG | - | Chr5:107730870-107730889 | MsG0580030171.01.T01:CDS | 30.0% | |
| GATGATGAAAATGGAGTTAA+TGG | - | Chr5:107731236-107731255 | MsG0580030171.01.T01:CDS | 30.0% | |
| GTAACAAATCTGCAAAAGAT+TGG | + | Chr5:107730680-107730699 | None:intergenic | 30.0% | |
| GTCAATATCACAAACCTAAA+AGG | - | Chr5:107730465-107730484 | MsG0580030171.01.T01:CDS | 30.0% | |
| GTTAACACTGCAAATATTGT+TGG | - | Chr5:107730981-107731000 | MsG0580030171.01.T01:CDS | 30.0% | |
| ! | TTCTACGGATTTTACGTAAA+AGG | + | Chr5:107730535-107730554 | None:intergenic | 30.0% |
| !! | AAACTTTTTGAGAGTCTGTT+AGG | + | Chr5:107730809-107730828 | None:intergenic | 30.0% |
| !! | ATCATATTTGTTATGGCCTT+CGG | + | Chr5:107730916-107730935 | None:intergenic | 30.0% |
| !! | CCAAGTGACATTGATTTAAT+CGG | - | Chr5:107730633-107730652 | MsG0580030171.01.T01:CDS | 30.0% |
| !! | GGAGTTAATGGATTAAATCA+AGG | - | Chr5:107731248-107731267 | MsG0580030171.01.T01:CDS | 30.0% |
| ACTCCCTTAAACAATTCTCT+TGG | + | Chr5:107731062-107731081 | None:intergenic | 35.0% | |
| ACTTTCAAAGAATCCGTTGA+CGG | - | Chr5:107730720-107730739 | MsG0580030171.01.T01:CDS | 35.0% | |
| ATTGTTTAAGGGAGTTAAGG+AGG | - | Chr5:107731067-107731086 | MsG0580030171.01.T01:CDS | 35.0% | |
| CCGATTAAATCAATGTCACT+TGG | + | Chr5:107730636-107730655 | None:intergenic | 35.0% | |
| GAGTGATAATATGAGTGTGA+GGG | + | Chr5:107730100-107730119 | None:intergenic | 35.0% | |
| GATGCCAAGAGAATTGTTTA+AGG | - | Chr5:107731055-107731074 | MsG0580030171.01.T01:CDS | 35.0% | |
| GATTTCACAGTGCAAAATGA+TGG | - | Chr5:107730933-107730952 | MsG0580030171.01.T01:CDS | 35.0% | |
| GTTTGTGATATTGACGTAAC+CGG | + | Chr5:107730460-107730479 | None:intergenic | 35.0% | |
| TCAAGGGTTGAGATTCATTA+TGG | - | Chr5:107731265-107731284 | MsG0580030171.01.T01:CDS | 35.0% | |
| TGAGTGATAATATGAGTGTG+AGG | + | Chr5:107730101-107730120 | None:intergenic | 35.0% | |
| ! | AAAGCTGCTGATGATGAAAA+TGG | - | Chr5:107731227-107731246 | MsG0580030171.01.T01:CDS | 35.0% |
| ! | CAACGGATTCTTTGAAAGTA+GGG | + | Chr5:107730719-107730738 | None:intergenic | 35.0% |
| ! | CAGTGTTAACTTTGGTTTCA+AGG | + | Chr5:107730971-107730990 | None:intergenic | 35.0% |
| ! | CAGTTTGTTTATTGGTGCTT+TGG | - | Chr5:107731295-107731314 | MsG0580030171.01.T01:CDS | 35.0% |
| ! | TCAACGGATTCTTTGAAAGT+AGG | + | Chr5:107730720-107730739 | None:intergenic | 35.0% |
| !! | AGGATTACTGATTTGAAGGA+CGG | + | Chr5:107730577-107730596 | None:intergenic | 35.0% |
| AACACCGTGGTATAATAGGA+GGG | + | Chr5:107730832-107730851 | None:intergenic | 40.0% | |
| AACCTAAAAGGTGGCAAAGT+CGG | - | Chr5:107730477-107730496 | MsG0580030171.01.T01:CDS | 40.0% | |
| ATGAACACATTAGCAACCGA+AGG | - | Chr5:107730897-107730916 | MsG0580030171.01.T01:CDS | 40.0% | |
| CATGTCTTAGTCGACTACTT+CGG | - | Chr5:107730351-107730370 | MsG0580030171.01.T01:CDS | 40.0% | |
| GACGTATGGAAGTTCTTCTA+CGG | + | Chr5:107730550-107730569 | None:intergenic | 40.0% | |
| GAGTAAGAGCAAGAAGAAGA+AGG | - | Chr5:107731130-107731149 | MsG0580030171.01.T01:CDS | 40.0% | |
| GTGAGGGAAAATAGAACAAG+TGG | + | Chr5:107730084-107730103 | None:intergenic | 40.0% | |
| GTTTCCCTCCTATTATACCA+CGG | - | Chr5:107730825-107730844 | MsG0580030171.01.T01:CDS | 40.0% | |
| TAAGAGCAAGAAGAAGAAGG+CGG | - | Chr5:107731133-107731152 | MsG0580030171.01.T01:CDS | 40.0% | |
| !!! | CTCTTACTCTTTGCTGCTTT+TGG | + | Chr5:107731119-107731138 | None:intergenic | 40.0% |
| AAACTCCACCAAATCACCGA+CGG | - | Chr5:107730381-107730400 | MsG0580030171.01.T01:CDS | 45.0% | |
| AACAGTTAAACCGCCGTCAA+CGG | + | Chr5:107730736-107730755 | None:intergenic | 45.0% | |
| AGTAGTCGACTAAGACATGG+AGG | + | Chr5:107730350-107730369 | None:intergenic | 45.0% | |
| ATGAAGACCGTCGTTGTCTT+CGG | + | Chr5:107730511-107730530 | None:intergenic | 45.0% | |
| ATTGGTATACTGGAACACCG+TGG | + | Chr5:107730845-107730864 | None:intergenic | 45.0% | |
| CGAAGTAGTCGACTAAGACA+TGG | + | Chr5:107730353-107730372 | None:intergenic | 45.0% | |
| CTGGAACACCGTGGTATAAT+AGG | + | Chr5:107730836-107730855 | None:intergenic | 45.0% | |
| GAACACCGTGGTATAATAGG+AGG | + | Chr5:107730833-107730852 | None:intergenic | 45.0% | |
| GGATGGTTATGGTTTGACGA+CGG | + | Chr5:107730251-107730270 | None:intergenic | 45.0% | |
| GTAGTCGACTAAGACATGGA+GGG | + | Chr5:107730349-107730368 | None:intergenic | 45.0% | |
| TCAGAATCTGCATCTGCATC+TGG | + | Chr5:107731200-107731219 | None:intergenic | 45.0% | |
| TGTGATATTGACGTAACCGG+CGG | + | Chr5:107730457-107730476 | None:intergenic | 45.0% | |
| TTCAAAGAATCCGTTGACGG+CGG | - | Chr5:107730723-107730742 | MsG0580030171.01.T01:CDS | 45.0% | |
| TTGACGACGGTTGATCTCAT+CGG | + | Chr5:107730238-107730257 | None:intergenic | 45.0% | |
| TTTGTCTAGGAGGGAAGACA+TGG | + | Chr5:107730292-107730311 | None:intergenic | 45.0% | |
| ! | GAGGGAGAGGACATTTTTGA+GGG | + | Chr5:107730331-107730350 | None:intergenic | 45.0% |
| ! | GATGGAAAGGTAGTGGTTGA+AGG | + | Chr5:107730205-107730224 | None:intergenic | 45.0% |
| !! | AAAGGTGGCAAAGTCGGTTT+TGG | - | Chr5:107730483-107730502 | MsG0580030171.01.T01:CDS | 45.0% |
| !! | AACCGACTTTGCCACCTTTT+AGG | + | Chr5:107730482-107730501 | None:intergenic | 45.0% |
| !! | GACATTTTTGAGGGTGGTGA+TGG | + | Chr5:107730322-107730341 | None:intergenic | 45.0% |
| !! | GGTGATTTGGTGGAGTTTCT+TGG | + | Chr5:107730379-107730398 | None:intergenic | 45.0% |
| !! | TGATGGAGAGGTGTTTGTCT+AGG | + | Chr5:107730305-107730324 | None:intergenic | 45.0% |
| CTAGAATGCGCGTGATGTTG+TGG | + | Chr5:107730161-107730180 | None:intergenic | 50.0% | |
| CTTTGCAGAGAAACCGCTGA+CGG | + | Chr5:107730778-107730797 | None:intergenic | 50.0% | |
| GAACATGGAGGAGACAAGTG+TGG | + | Chr5:107730409-107730428 | None:intergenic | 50.0% | |
| GTCGATGGCTAGGATGGTTA+TGG | + | Chr5:107730262-107730281 | None:intergenic | 50.0% | |
| TCCTCCATGTTCCAAGCTAC+CGG | - | Chr5:107730417-107730436 | MsG0580030171.01.T01:CDS | 50.0% | |
| TGAAGGACGGAGATGACGTA+TGG | + | Chr5:107730564-107730583 | None:intergenic | 50.0% | |
| ! | CGCATTCTAGCTAAACACCC+TGG | - | Chr5:107730171-107730190 | MsG0580030171.01.T01:CDS | 50.0% |
| ! | GGAAAGGTAGTGGTTGAAGG+TGG | + | Chr5:107730202-107730221 | None:intergenic | 50.0% |
| ! | GGAGGGAGAGGACATTTTTG+AGG | + | Chr5:107730332-107730351 | None:intergenic | 50.0% |
| !! | GGAGAGGACATTTTTGAGGG+TGG | + | Chr5:107730328-107730347 | None:intergenic | 50.0% |
| !! | GGAGAGGTGTTTGTCTAGGA+GGG | + | Chr5:107730301-107730320 | None:intergenic | 50.0% |
| !! | TGGAGAGGTGTTTGTCTAGG+AGG | + | Chr5:107730302-107730321 | None:intergenic | 50.0% |
| !! | TTTGGTGCCGAAGACAACGA+CGG | - | Chr5:107730501-107730520 | MsG0580030171.01.T01:CDS | 50.0% |
| !!! | CTCTTTGCTGCTTTTGGTGC+TGG | + | Chr5:107731113-107731132 | None:intergenic | 50.0% |
| !!! | TTTTGAGGGTGGTGATGGAG+AGG | + | Chr5:107730317-107730336 | None:intergenic | 50.0% |
| AGACATGGCGGCATTGTCGA+TGG | + | Chr5:107730277-107730296 | None:intergenic | 55.0% | |
| CGACTAAGACATGGAGGGAG+AGG | + | Chr5:107730344-107730363 | None:intergenic | 55.0% | |
| GGAGACAAGTGTGGTACCGT+CGG | + | Chr5:107730400-107730419 | None:intergenic | 55.0% | |
| GGATCCGGTAGCTTGGAACA+TGG | + | Chr5:107730424-107730443 | None:intergenic | 55.0% | |
| GGATGAGGATGCAGACTCAC+CGG | - | Chr5:107731166-107731185 | MsG0580030171.01.T01:CDS | 55.0% | |
| GGCATTGTCGATGGCTAGGA+TGG | + | Chr5:107730268-107730287 | None:intergenic | 55.0% | |
| GGTTGAAGGTGGAGAAGCCA+GGG | + | Chr5:107730191-107730210 | None:intergenic | 55.0% | |
| GTCTAGGAGGGAAGACATGG+CGG | + | Chr5:107730289-107730308 | None:intergenic | 55.0% | |
| GTGTGGTACCGTCGGTGATT+TGG | + | Chr5:107730392-107730411 | None:intergenic | 55.0% | |
| GTTGATCTCATCGGCGAGGT+GGG | + | Chr5:107730229-107730248 | None:intergenic | 55.0% | |
| TCCGGTAGCTTGGAACATGG+AGG | + | Chr5:107730421-107730440 | None:intergenic | 55.0% | |
| TCTGCATCTGGTCCATCTGC+CGG | + | Chr5:107731188-107731207 | None:intergenic | 55.0% | |
| TGGTTGAAGGTGGAGAAGCC+AGG | + | Chr5:107730192-107730211 | None:intergenic | 55.0% | |
| ! | ATGTCACTTGGAGCTGCCGT+CGG | + | Chr5:107730624-107730643 | None:intergenic | 55.0% |
| ! | TGGTACCGTCGGTGATTTGG+TGG | + | Chr5:107730389-107730408 | None:intergenic | 55.0% |
| !! | GGTGGGTGATGGAAAGGTAG+TGG | + | Chr5:107730212-107730231 | None:intergenic | 55.0% |
| CAAGAAGAAGAAGGCGGCGC+CGG | - | Chr5:107731139-107731158 | MsG0580030171.01.T01:CDS | 60.0% | |
| CCAACAGATACCGCCGTCAG+CGG | - | Chr5:107730762-107730781 | MsG0580030171.01.T01:CDS | 60.0% | |
| CGTGATGTTGTGGTTGCCGG+AGG | + | Chr5:107730151-107730170 | None:intergenic | 60.0% | |
| GACGGTTGATCTCATCGGCG+AGG | + | Chr5:107730233-107730252 | None:intergenic | 60.0% | |
| GATGCAGACTCACCGGCAGA+TGG | - | Chr5:107731173-107731192 | MsG0580030171.01.T01:CDS | 60.0% | |
| GATGTTGTGGTTGCCGGAGG+TGG | + | Chr5:107730148-107730167 | None:intergenic | 60.0% | |
| GCGCGTGATGTTGTGGTTGC+CGG | + | Chr5:107730154-107730173 | None:intergenic | 60.0% | |
| GGTTGATCTCATCGGCGAGG+TGG | + | Chr5:107730230-107730249 | None:intergenic | 60.0% | |
| TGCAGAGAAACCGCTGACGG+CGG | + | Chr5:107730775-107730794 | None:intergenic | 60.0% | |
| TGGCGGCATTGTCGATGGCT+AGG | + | Chr5:107730272-107730291 | None:intergenic | 60.0% | |
| ! | TCTGCGTCGGCGGAGCTTAA+AGG | + | Chr5:107730597-107730616 | None:intergenic | 60.0% |
| !!! | GCTGCTTTTGGTGCTGGAGC+TGG | + | Chr5:107731107-107731126 | None:intergenic | 60.0% |
| CCGCTGACGGCGGTATCTGT+TGG | + | Chr5:107730765-107730784 | None:intergenic | 65.0% | |
| CTCATCGGCGAGGTGGGTGA+TGG | + | Chr5:107730223-107730242 | None:intergenic | 65.0% | |
| GACGTAACCGGCGGTTCCAG+CGG | + | Chr5:107730448-107730467 | None:intergenic | 65.0% | |
| GTTGTGGTTGCCGGAGGTGG+CGG | + | Chr5:107730145-107730164 | None:intergenic | 65.0% | |
| TCTGCATCCTCATCCGCCGC+CGG | + | Chr5:107731161-107731180 | None:intergenic | 65.0% | |
| !! | CGGCGAGGTGGGTGATGGAA+AGG | + | Chr5:107730218-107730237 | None:intergenic | 65.0% |
| CAAGCTACCGGATCCGCCGC+TGG | - | Chr5:107730429-107730448 | MsG0580030171.01.T01:CDS | 70.0% | |
| CAGCGGCGGATCCGGTAGCT+TGG | + | Chr5:107730431-107730450 | None:intergenic | 70.0% | |
| GTAACCGGCGGTTCCAGCGG+CGG | + | Chr5:107730445-107730464 | None:intergenic | 70.0% | |
| TGCCGTCGGCGCTTCTGCGT+CGG | + | Chr5:107730610-107730629 | None:intergenic | 70.0% | |
| ! | GAAGAAGAAGGCGGCGCCGG+CGG | - | Chr5:107731142-107731161 | MsG0580030171.01.T01:CDS | 70.0% |
| ATCGCCGCTGCCGCCACCTC+CGG | - | Chr5:107730132-107730151 | MsG0580030171.01.T01:CDS | 75.0% | |
| CGTCGGCGCTTCTGCGTCGG+CGG | + | Chr5:107730607-107730626 | None:intergenic | 75.0% | |
| GGATCCGCCGCTGGAACCGC+CGG | - | Chr5:107730438-107730457 | MsG0580030171.01.T01:CDS | 75.0% | |
| GGCGGTTCCAGCGGCGGATC+CGG | + | Chr5:107730439-107730458 | None:intergenic | 75.0% | |
| ! | GTTGCCGGAGGTGGCGGCAG+CGG | + | Chr5:107730139-107730158 | None:intergenic | 75.0% |
| !! | CGCCGACGCAGAAGCGCCGA+CGG | - | Chr5:107730605-107730624 | MsG0580030171.01.T01:CDS | 75.0% |
| !! | GGAGGTGGCGGCAGCGGCGA+TGG | + | Chr5:107730133-107730152 | None:intergenic | 80.0% |
| !! | GGTGGCGGCAGCGGCGATGG+TGG | + | Chr5:107730130-107730149 | None:intergenic | 80.0% |
| !!! | GAAGAAGGCGGCGCCGGCGG+CGG | - | Chr5:107731145-107731164 | MsG0580030171.01.T01:CDS | 80.0% |
| !!! | GGCGGCGCCGGCGGCGGATG+AGG | - | Chr5:107731151-107731170 | MsG0580030171.01.T01:CDS | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 107730063 | 107731325 | 107730063 | ID=MsG0580030171.01;Name=MsG0580030171.01 |
| Chr5 | mRNA | 107730063 | 107731325 | 107730063 | ID=MsG0580030171.01.T01;Parent=MsG0580030171.01;Name=MsG0580030171.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|420 |
| Chr5 | exon | 107730063 | 107731325 | 107730063 | ID=MsG0580030171.01.T01:exon:14927;Parent=MsG0580030171.01.T01 |
| Chr5 | CDS | 107730063 | 107731325 | 107730063 | ID=MsG0580030171.01.T01:cds;Parent=MsG0580030171.01.T01 |
| Gene Sequence |
| Protein sequence |
