AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030524.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030524.01.T01 MTR_3g101100 67.290 107 27 1 1 99 1289 1395 1.15e-41 149
MsG0680030524.01.T01 MTR_6g017010 71.569 102 19 1 1 92 664 765 3.29e-41 147
MsG0680030524.01.T01 MTR_3g107960 64.486 107 30 1 1 99 1100 1206 8.95e-41 146
MsG0680030524.01.T01 MTR_5g085310 76.667 90 13 1 2 83 936 1025 1.73e-40 145
MsG0680030524.01.T01 MTR_6g017130 85.246 61 8 1 22 81 330 390 2.68e-29 111
MsG0680030524.01.T01 MTR_6g089560 57.333 75 32 0 102 176 117 191 1.93e-26 100
MsG0680030524.01.T01 MTR_1g017840 53.333 75 35 0 102 176 79 153 9.81e-25 94.4
MsG0680030524.01.T01 MTR_0093s0070 53.846 78 36 0 99 176 121 198 1.06e-23 92.8
MsG0680030524.01.T01 MTR_1g047940 58.242 91 18 2 1 89 126 198 1.16e-23 92.8
MsG0680030524.01.T01 MTR_7g013520 48.235 85 44 0 92 176 61 145 6.81e-23 90.1
MsG0680030524.01.T01 MTR_8g099580 50.000 84 42 0 93 176 198 281 2.11e-22 91.7
MsG0680030524.01.T01 MTR_8g098925 35.507 138 80 3 47 176 4 140 3.38e-22 87.8
MsG0680030524.01.T01 MTR_4g005050 44.681 94 47 1 3 91 1602 1695 3.95e-21 90.1
MsG0680030524.01.T01 MTR_1g022215 57.143 56 24 0 102 157 15 70 1.31e-19 79.0
MsG0680030524.01.T01 MTR_5g037920 54.167 72 32 1 105 176 102 172 1.74e-19 82.4
MsG0680030524.01.T01 MTR_5g034450 53.333 60 28 0 101 160 49 108 3.26e-17 73.6
MsG0680030524.01.T01 MTR_2g017550 50.820 61 30 0 93 153 100 160 3.56e-16 72.4
MsG0680030524.01.T01 MTR_8g059135 47.222 72 38 0 104 175 31 102 1.07e-15 70.1
MsG0680030524.01.T01 MTR_6g016265 44.048 84 47 0 93 176 31 114 1.22e-15 69.7
MsG0680030524.01.T01 MTR_4g029360 45.455 77 42 0 100 176 126 202 1.73e-15 71.6
MsG0680030524.01.T01 MTR_6g033225 44.776 67 37 0 103 169 72 138 8.29e-15 68.2
MsG0680030524.01.T01 MTR_4g008270 40.789 76 45 0 101 176 10 85 1.98e-14 65.9
MsG0680030524.01.T01 MTR_7g063280 40.789 76 45 0 101 176 72 147 2.05e-14 67.8
MsG0680030524.01.T01 MTR_6g453270 43.056 72 41 0 105 176 304 375 2.15e-14 70.1
MsG0680030524.01.T01 MTR_2g055490 52.632 57 27 0 106 162 12 68 3.31e-14 65.9
MsG0680030524.01.T01 MTR_5g049680 36.471 85 53 1 93 176 23 107 2.04e-13 63.5
MsG0680030524.01.T01 MTR_3g116210 36.905 84 53 0 93 176 89 172 2.43e-13 65.5
MsG0680030524.01.T01 MTR_1g088650 43.836 73 41 0 104 176 89 161 2.70e-13 65.1
MsG0680030524.01.T01 MTR_2g059470 40.541 74 44 0 103 176 2 75 4.07e-13 62.0
MsG0680030524.01.T01 MTR_1g018300 45.238 84 40 1 93 176 55 132 4.20e-13 65.9
MsG0680030524.01.T01 MTR_5g075610 43.939 66 35 1 107 172 1 64 7.06e-13 62.0
MsG0680030524.01.T01 MTR_1g069475 38.356 73 45 0 104 176 167 239 1.15e-12 64.3
MsG0680030524.01.T01 MTR_2g027020 40.789 76 41 1 101 176 28 99 1.89e-12 62.8
MsG0680030524.01.T01 MTR_5g083200 39.726 73 44 0 104 176 101 173 2.18e-12 62.8
MsG0680030524.01.T01 MTR_0002s1220 39.726 73 44 0 104 176 101 173 2.18e-12 62.8
MsG0680030524.01.T01 MTR_6g045427 62.791 43 16 0 134 176 178 220 2.67e-12 63.2
MsG0680030524.01.T01 MTR_3g111390 39.726 73 44 0 104 176 101 173 3.18e-12 62.4
MsG0680030524.01.T01 MTR_1g070820 39.726 73 44 0 104 176 101 173 3.18e-12 62.4
MsG0680030524.01.T01 MTR_1g045340 39.726 73 44 0 104 176 87 159 3.87e-12 62.0
MsG0680030524.01.T01 MTR_1g050488 34.667 75 49 0 93 167 90 164 1.32e-11 60.1
MsG0680030524.01.T01 MTR_5g015680 35.644 101 53 5 16 112 188 280 1.39e-11 61.6
MsG0680030524.01.T01 MTR_5g015680 35.644 101 53 5 16 112 283 375 1.60e-11 62.0
MsG0680030524.01.T01 MTR_1g034430 24.590 122 89 1 55 176 52 170 1.81e-11 60.1
MsG0680030524.01.T01 MTR_5g015680 35.644 101 53 5 16 112 322 414 1.82e-11 62.0
MsG0680030524.01.T01 MTR_5g036320 50.000 60 30 0 101 160 79 138 4.45e-11 58.2
MsG0680030524.01.T01 MTR_0015s0120 38.272 81 50 0 93 173 285 365 8.35e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1573 1666 2.02e-24 99.8
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1573 1666 2.02e-24 99.8
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1573 1666 2.02e-24 99.8
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1573 1666 2.02e-24 99.8
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1570 1663 2.08e-24 99.8
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1599 1692 2.08e-24 99.8
MsG0680030524.01.T01 AT1G79000 46.809 94 45 2 3 91 1590 1683 2.29e-24 99.4
MsG0680030524.01.T01 AT1G79000 46.809 94 45 2 3 91 1590 1683 2.32e-24 99.4
MsG0680030524.01.T01 AT1G79000 46.809 94 45 2 3 91 1566 1659 2.36e-24 99.4
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1534 1627 2.72e-24 99.4
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1534 1627 2.72e-24 99.4
MsG0680030524.01.T01 AT1G16710 44.681 94 47 1 3 91 1534 1627 2.72e-24 99.4
MsG0680030524.01.T01 AT3G12980 38.298 94 50 2 3 91 1565 1655 7.42e-16 75.1
MsG0680030524.01.T01 AT3G12980 38.298 94 50 2 3 91 1484 1574 8.79e-16 74.7
MsG0680030524.01.T01 AT5G67480 43.939 66 36 1 19 83 280 345 7.92e-12 62.8
MsG0680030524.01.T01 AT5G67480 43.939 66 36 1 19 83 280 345 7.92e-12 62.8
MsG0680030524.01.T01 AT5G67480 43.939 66 36 1 19 83 291 356 8.01e-12 62.8
MsG0680030524.01.T01 AT1G55970 34.043 94 59 1 11 104 1380 1470 9.97e-11 60.1

Find 23 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCTATTTGTGAAGCTTT+AGG 0.291360 6:+4645876 MsG0680030524.01.T01:CDS
TTCCGAGTCTCTGCAATTCC+TGG 0.327710 6:-4644192 None:intergenic
AGATTTCAGCGTACCGGTAT+TGG 0.360138 6:+4645783 MsG0680030524.01.T01:CDS
TTGTCGTAGAGCTGAAGTTT+TGG 0.361135 6:+4644415 MsG0680030524.01.T01:CDS
AATGTAGAATACCGCGTTGT+AGG 0.362248 6:+4644213 MsG0680030524.01.T01:CDS
GTGTCTATCATGTTATTCAA+TGG 0.423944 6:+4645898 MsG0680030524.01.T01:CDS
AAAGCTTCACAAATAGCAAC+TGG 0.432607 6:-4645873 None:intergenic
AAGTGCAAAATTCGAATGAA+TGG 0.438598 6:+4644122 MsG0680030524.01.T01:CDS
AGTGCAAAATTCGAATGAAT+GGG 0.457982 6:+4644123 MsG0680030524.01.T01:CDS
GAAAGCGACGGAGCAGTCAT+TGG 0.464537 6:+4644437 MsG0680030524.01.T01:CDS
TGACATCCACCGTGCGGGAA+TGG 0.473452 6:-4645986 None:intergenic
TTATACTAGCTACGCACTCC+AGG 0.495911 6:+4644174 MsG0680030524.01.T01:CDS
TCAGAGAGATTTCAGCGTAC+CGG 0.517168 6:+4645777 MsG0680030524.01.T01:intron
CTCCAGGAATTGCAGAGACT+CGG 0.528433 6:+4644190 MsG0680030524.01.T01:CDS
TTCTATGACATCCACCGTGC+GGG 0.530948 6:-4645991 None:intergenic
ACGAATACAAATTCCAATAC+CGG 0.562322 6:-4645796 None:intergenic
TTTAGCTAAAACAAATATGA+CGG 0.596720 6:-4645826 None:intergenic
GTGCAAAATTCGAATGAATG+GGG 0.623329 6:+4644124 MsG0680030524.01.T01:CDS
TGCTTTCCATTCCCGCACGG+TGG 0.634709 6:+4645980 MsG0680030524.01.T01:CDS
TGCAAAATTCGAATGAATGG+GGG 0.645250 6:+4644125 MsG0680030524.01.T01:CDS
AGATGCTTTCCATTCCCGCA+CGG 0.679367 6:+4645977 MsG0680030524.01.T01:CDS
ATTCTATGACATCCACCGTG+CGG 0.701414 6:-4645992 None:intergenic
ATTGTAACACACCTACAACG+CGG 0.798908 6:-4644224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATTTATATAAACAAA+AGG - Chr6:4644621-4644640 None:intergenic 10.0%
!! AAATTATGATCATAAAGAAA+TGG - Chr6:4645688-4645707 None:intergenic 15.0%
!!! CTAAATTTTATCTATGATAA+TGG + Chr6:4645158-4645177 MsG0680030524.01.T01:intron 15.0%
!! AAAAAATAGGAAACTATTAG+TGG + Chr6:4645550-4645569 MsG0680030524.01.T01:intron 20.0%
!! AATATAAGAGTAATTGACAA+AGG - Chr6:4645052-4645071 None:intergenic 20.0%
!! ATATAAGAGTAATTGACAAA+GGG - Chr6:4645051-4645070 None:intergenic 20.0%
!! TTGTTGTGAATATATTTCAA+TGG + Chr6:4644710-4644729 MsG0680030524.01.T01:intron 20.0%
!! TTTAGCTAAAACAAATATGA+CGG - Chr6:4645829-4645848 None:intergenic 20.0%
!!! ATTGAATCTCTTATTTTAAG+AGG - Chr6:4644684-4644703 None:intergenic 20.0%
! AAAAAGACACTAAAAAGGAA+CGG - Chr6:4644961-4644980 None:intergenic 25.0%
! AAACACAAAGAACAAATCAA+TGG + Chr6:4644871-4644890 MsG0680030524.01.T01:intron 25.0%
! ACACAAGAATAATGAAACTA+TGG - Chr6:4644915-4644934 None:intergenic 25.0%
! GTAGAATATCAAAATTCTCT+AGG - Chr6:4644265-4644284 None:intergenic 25.0%
! TAATGTGTGTGCAAAAAAAT+AGG + Chr6:4645537-4645556 MsG0680030524.01.T01:intron 25.0%
! TACCAAAATGACAGTTAAAT+AGG - Chr6:4645001-4645020 None:intergenic 25.0%
! TTTGATTATGTGACATACAT+AGG + Chr6:4644635-4644654 MsG0680030524.01.T01:intron 25.0%
!! ACCATATCTTTTTACAATGT+TGG - Chr6:4644154-4644173 None:intergenic 25.0%
!! GATTAAAATGAGGGTATAAT+AGG + Chr6:4645572-4645591 MsG0680030524.01.T01:intron 25.0%
!!! ACTATTAGTGGATTAAAATG+AGG + Chr6:4645562-4645581 MsG0680030524.01.T01:intron 25.0%
!!! CTATTAGTGGATTAAAATGA+GGG + Chr6:4645563-4645582 MsG0680030524.01.T01:intron 25.0%
!!! CTGTCATTTTGGTATTTTAA+AGG + Chr6:4645007-4645026 MsG0680030524.01.T01:intron 25.0%
!!! TATAGAATTTTAACCCTTCA+TGG - Chr6:4645186-4645205 None:intergenic 25.0%
!!! TCCTTTTTAGTGTCTTTTTA+GGG + Chr6:4644962-4644981 MsG0680030524.01.T01:intron 25.0%
!!! TTCCTATTTAACTGTCATTT+TGG + Chr6:4644996-4645015 MsG0680030524.01.T01:intron 25.0%
!!! TTCCTTTTTAGTGTCTTTTT+AGG + Chr6:4644961-4644980 MsG0680030524.01.T01:intron 25.0%
!!! TTCTAAAACGACACTTAAAA+AGG + Chr6:4645637-4645656 MsG0680030524.01.T01:intron 25.0%
AAACGACACTTAAAAAGGAA+CGG + Chr6:4645642-4645661 MsG0680030524.01.T01:intron 30.0%
AAGTGCAAAATTCGAATGAA+TGG + Chr6:4644122-4644141 MsG0680030524.01.T01:CDS 30.0%
ACCCTAAAAAGACACTAAAA+AGG - Chr6:4644966-4644985 None:intergenic 30.0%
ACGAATACAAATTCCAATAC+CGG - Chr6:4645799-4645818 None:intergenic 30.0%
AGAAAGAGTTGAAGATTCAT+TGG + Chr6:4645302-4645321 MsG0680030524.01.T01:intron 30.0%
AGTGCAAAATTCGAATGAAT+GGG + Chr6:4644123-4644142 MsG0680030524.01.T01:CDS 30.0%
CGCAAAAAATAGGAATTTGT+TGG + Chr6:4645459-4645478 MsG0680030524.01.T01:intron 30.0%
GATAGCATTTCATTCATGTT+TGG + Chr6:4644845-4644864 MsG0680030524.01.T01:intron 30.0%
GCCAACATTGTAAAAAGATA+TGG + Chr6:4644150-4644169 MsG0680030524.01.T01:CDS 30.0%
GTGTCTATCATGTTATTCAA+TGG + Chr6:4645898-4645917 MsG0680030524.01.T01:CDS 30.0%
TGAATGAAATGCTATCAATG+TGG - Chr6:4644841-4644860 None:intergenic 30.0%
TGAGACTTGAATTGAATAGT+TGG - Chr6:4644751-4644770 None:intergenic 30.0%
TTATGTGACATACATAGGTT+AGG + Chr6:4644640-4644659 MsG0680030524.01.T01:intron 30.0%
!! GCTTTTGATTATTGTGAAGT+TGG + Chr6:4645346-4645365 MsG0680030524.01.T01:intron 30.0%
!! TATGATAATGGTTCCATGAA+GGG + Chr6:4645170-4645189 MsG0680030524.01.T01:intron 30.0%
AAAGCTTCACAAATAGCAAC+TGG - Chr6:4645876-4645895 None:intergenic 35.0%
AAGAGTTGAAGATTCATTGG+AGG + Chr6:4645305-4645324 MsG0680030524.01.T01:intron 35.0%
AGATAAAGCGCGCAAAAAAT+AGG + Chr6:4645449-4645468 MsG0680030524.01.T01:intron 35.0%
GAATTGAATAGTTGGAGCTT+AGG - Chr6:4644743-4644762 None:intergenic 35.0%
GTGCAAAATTCGAATGAATG+GGG + Chr6:4644124-4644143 MsG0680030524.01.T01:CDS 35.0%
TGCAAAATTCGAATGAATGG+GGG + Chr6:4644125-4644144 MsG0680030524.01.T01:CDS 35.0%
! CATTCGAATTTTGCACTTAC+TGG - Chr6:4644120-4644139 None:intergenic 35.0%
! TTCTTCCTTTTGAGCATTCA+TGG - Chr6:4645286-4645305 None:intergenic 35.0%
!! CTATGATAATGGTTCCATGA+AGG + Chr6:4645169-4645188 MsG0680030524.01.T01:intron 35.0%
!! GTTGCTATTTGTGAAGCTTT+AGG + Chr6:4645876-4645895 MsG0680030524.01.T01:CDS 35.0%
AAGACACTAAAAAGGAACGG+AGG - Chr6:4644958-4644977 None:intergenic 40.0%
AATGTAGAATACCGCGTTGT+AGG + Chr6:4644213-4644232 MsG0680030524.01.T01:CDS 40.0%
AGACACTAAAAAGGAACGGA+GGG - Chr6:4644957-4644976 None:intergenic 40.0%
AGACTCCATGAATGCTCAAA+AGG + Chr6:4645278-4645297 MsG0680030524.01.T01:intron 40.0%
ATTGTAACACACCTACAACG+CGG - Chr6:4644227-4644246 None:intergenic 40.0%
GACACTTAAAAAGGAACGGA+GGG + Chr6:4645646-4645665 MsG0680030524.01.T01:intron 40.0%
! ACGCTGAAATCTCTCTGAAA+AGG - Chr6:4645775-4645794 None:intergenic 40.0%
! TTGTCGTAGAGCTGAAGTTT+TGG + Chr6:4644415-4644434 MsG0680030524.01.T01:CDS 40.0%
ATTCTATGACATCCACCGTG+CGG - Chr6:4645995-4646014 None:intergenic 45.0%
CGACACTTAAAAAGGAACGG+AGG + Chr6:4645645-4645664 MsG0680030524.01.T01:intron 45.0%
TCAGAGAGATTTCAGCGTAC+CGG + Chr6:4645777-4645796 MsG0680030524.01.T01:intron 45.0%
TTATACTAGCTACGCACTCC+AGG + Chr6:4644174-4644193 MsG0680030524.01.T01:CDS 45.0%
! AGATTTCAGCGTACCGGTAT+TGG + Chr6:4645783-4645802 MsG0680030524.01.T01:CDS 45.0%
!!! GCTGAAGTTTTGGAAAGCGA+CGG + Chr6:4644425-4644444 MsG0680030524.01.T01:CDS 45.0%
AGATGCTTTCCATTCCCGCA+CGG + Chr6:4645977-4645996 MsG0680030524.01.T01:CDS 50.0%
CTCCAGGAATTGCAGAGACT+CGG + Chr6:4644190-4644209 MsG0680030524.01.T01:CDS 50.0%
TTCCGAGTCTCTGCAATTCC+TGG - Chr6:4644195-4644214 None:intergenic 50.0%
TTCTATGACATCCACCGTGC+GGG - Chr6:4645994-4646013 None:intergenic 50.0%
GAAAGCGACGGAGCAGTCAT+TGG + Chr6:4644437-4644456 MsG0680030524.01.T01:CDS 55.0%
TGACATCCACCGTGCGGGAA+TGG - Chr6:4645989-4646008 None:intergenic 60.0%
TGCTTTCCATTCCCGCACGG+TGG + Chr6:4645980-4645999 MsG0680030524.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 4644032 4646019 4644032 ID=MsG0680030524.01;Name=MsG0680030524.01
Chr6 mRNA 4644032 4646019 4644032 ID=MsG0680030524.01.T01;Parent=MsG0680030524.01;Name=MsG0680030524.01.T01;_AED=0.44;_eAED=0.45;_QI=0|0|0|0.66|0|0|3|0|176
Chr6 exon 4644032 4644234 4644032 ID=MsG0680030524.01.T01:exon:12391;Parent=MsG0680030524.01.T01
Chr6 exon 4644370 4644458 4644370 ID=MsG0680030524.01.T01:exon:12390;Parent=MsG0680030524.01.T01
Chr6 exon 4645781 4646019 4645781 ID=MsG0680030524.01.T01:exon:12389;Parent=MsG0680030524.01.T01
Chr6 CDS 4644032 4644234 4644032 ID=MsG0680030524.01.T01:cds;Parent=MsG0680030524.01.T01
Chr6 CDS 4644370 4644458 4644370 ID=MsG0680030524.01.T01:cds;Parent=MsG0680030524.01.T01
Chr6 CDS 4645781 4646019 4645781 ID=MsG0680030524.01.T01:cds;Parent=MsG0680030524.01.T01
Gene Sequence

>MsG0680030524.01.T01

ATGGTGAAACTGATGAACGTTTTGAAGCATGCATCTCAATACGCTCATTCAAACTGTTCACAGATCAATAAGTTGTTCTCTCATGCCAGTAAGTGCAAAATTCGAATGAATGGGGGCTGCCAACATTGTAAAAAGATATGGTTTATACTAGCTACGCACTCCAGGAATTGCAGAGACTCGGAATGTAGAATACCGCGTTGTAGTGATTTGAAGAAGCATGTAGAACTGAGATCAATGCATTCTGAATCTTGTCGTAGAGCTGAAGTTTTGGAAAGCGACGGAGCAGTCATTGAGAGATTTCAGCGTACCGGTATTGGAATTTGTATTCGTGACGAGTCCGTCATATTTGTTTTAGCTAAAGTGCTGATGTTTTGTCAAATATATCCAGTTGCTATTTGTGAAGCTTTAGGTGTCTATCATGTTATTCAATGGTTGCAAGATATGCGTTTTGACAATATAGACTTCGAGCTTGATTCTAAAATAACGAGAGATGCTTTCCATTCCCGCACGGTGGATGTCATAGAATTTTGA

Protein sequence

>MsG0680030524.01.T01

MVKLMNVLKHASQYAHSNCSQINKLFSHASKCKIRMNGGCQHCKKIWFILATHSRNCRDSECRIPRCSDLKKHVELRSMHSESCRRAEVLESDGAVIERFQRTGIGICIRDESVIFVLAKVLMFCQIYPVAICEALGVYHVIQWLQDMRFDNIDFELDSKITRDAFHSRTVDVIEF*