AlfalfaGEDB | MsG0680030605.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030605.01

KEGG: -
GO: GO:0016020 GO:0016021
PFAM: PF00892

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030605.01.T01	GAU47308.1	86.957	161	21	0	6	166	209	369	1.74E-90	278

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030605.01.T01	Q94JU2	42.331	163	81	3	7	156	202	364	2.46E-37	134

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030605.01.T01	A0A2Z6NSX8	86.957	161	21	0	6	166	209	369	8.31e-91	278

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0680030605.01	MsG0680030606.01	0.873572	1.325735e-67	5.781235e-64
MsG0680030605.01	MsG0680030607.01	0.841470	4.660707e-58	6.855623e-55
MsG0680030605.01	MsG0880043298.01	0.807430	5.075208e-50	2.826217e-47
MsG0280007202.01	MsG0680030605.01	0.824623	7.299824e-54	6.501517e-51
MsG0280008343.01	MsG0680030605.01	0.802353	5.839972e-49	2.854646e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030605.01.T01	MTR_6g007757	93.210	162	10	1	6	166	204	365	8.85e-91	269
MsG0680030605.01.T01	MTR_6g007743	83.648	159	26	0	6	164	203	361	1.64e-88	263
MsG0680030605.01.T01	MTR_6g007753	83.230	161	27	0	6	166	163	323	1.04e-87	269
MsG0680030605.01.T01	MTR_6g007753	83.230	161	27	0	6	166	487	647	1.04e-87	269
MsG0680030605.01.T01	MTR_6g007747	83.436	163	27	0	4	166	199	361	1.50e-85	255
MsG0680030605.01.T01	MTR_6g007750	80.124	161	32	0	6	166	206	366	3.08e-78	237
MsG0680030605.01.T01	MTR_6g007740	82.500	160	28	0	6	165	203	362	5.84e-78	239
MsG0680030605.01.T01	MTR_0103s0090	55.280	161	72	0	6	166	113	273	1.14e-61	192
MsG0680030605.01.T01	MTR_0103s0090	55.280	161	72	0	6	166	204	364	8.40e-61	192
MsG0680030605.01.T01	MTR_1g014510	45.205	146	78	1	8	151	210	355	9.17e-40	139
MsG0680030605.01.T01	MTR_6g007760	57.143	105	45	0	6	110	205	309	1.56e-34	123
MsG0680030605.01.T01	MTR_4g106720	41.096	146	86	0	7	152	207	352	3.39e-33	120
MsG0680030605.01.T01	MTR_5g034980	44.355	124	69	0	8	131	203	326	1.09e-31	116
MsG0680030605.01.T01	MTR_3g090550	43.624	149	80	3	8	156	199	343	1.58e-30	113
MsG0680030605.01.T01	MTR_5g034990	38.667	150	90	2	4	152	202	350	1.16e-26	103
MsG0680030605.01.T01	MTR_5g034910	40.323	124	74	0	8	131	203	326	8.12e-26	100
MsG0680030605.01.T01	MTR_3g072560	39.416	137	82	1	9	145	213	348	3.01e-25	100
MsG0680030605.01.T01	MTR_5g034930	40.650	123	73	0	9	131	203	325	2.90e-24	97.1
MsG0680030605.01.T01	MTR_6g007757	90.566	53	5	0	6	58	204	256	5.71e-23	92.0
MsG0680030605.01.T01	MTR_4g094362	34.815	135	86	1	10	142	214	348	1.27e-21	90.1
MsG0680030605.01.T01	MTR_2g007970	39.200	125	76	0	10	134	212	336	1.69e-21	89.7
MsG0680030605.01.T01	MTR_3g095460	33.824	136	90	0	8	143	204	339	3.60e-21	88.6
MsG0680030605.01.T01	MTR_3g072530	35.294	136	86	1	9	142	211	346	4.67e-21	88.2
MsG0680030605.01.T01	MTR_3g072500	37.037	135	83	1	9	141	116	250	6.61e-21	87.0
MsG0680030605.01.T01	MTR_2g007990	35.156	128	79	2	10	135	216	341	8.00e-21	88.2
MsG0680030605.01.T01	MTR_3g072500	37.037	135	83	1	9	141	219	353	1.44e-20	87.4
MsG0680030605.01.T01	MTR_2g007260	33.333	126	84	0	10	135	219	344	6.22e-20	85.5
MsG0680030605.01.T01	MTR_3g012420	37.500	128	80	0	15	142	222	349	6.92e-20	85.5
MsG0680030605.01.T01	MTR_4g094340	33.099	142	92	1	12	150	145	286	8.95e-20	84.0
MsG0680030605.01.T01	MTR_0096s0100	33.566	143	92	2	9	150	214	354	1.00e-19	84.7
MsG0680030605.01.T01	MTR_4g094340	32.867	143	93	1	12	151	229	371	1.62e-19	84.3
MsG0680030605.01.T01	MTR_4g094340	32.867	143	93	1	12	151	211	353	1.63e-19	84.0
MsG0680030605.01.T01	MTR_1g074120	35.915	142	89	2	9	149	211	351	1.86e-19	84.0
MsG0680030605.01.T01	MTR_1g030580	35.461	141	87	1	9	149	213	349	2.65e-19	83.6
MsG0680030605.01.T01	MTR_4g094360	32.432	148	95	1	10	152	168	315	2.73e-19	83.6
MsG0680030605.01.T01	MTR_2g027030	37.190	121	76	0	10	130	212	332	2.80e-19	83.6
MsG0680030605.01.T01	MTR_4g094360	32.432	148	95	1	10	152	215	362	4.45e-19	83.2
MsG0680030605.01.T01	MTR_1g052205	34.247	146	90	3	9	150	89	232	7.87e-19	80.9
MsG0680030605.01.T01	MTR_2g102340	36.429	140	85	2	10	145	209	348	1.01e-18	82.0
MsG0680030605.01.T01	MTR_8g041390	35.156	128	83	0	15	142	221	348	1.32e-18	82.0
MsG0680030605.01.T01	MTR_2g007960	31.690	142	96	1	10	151	218	358	1.58e-18	81.6
MsG0680030605.01.T01	MTR_8g006010	38.017	121	75	0	13	133	211	331	2.24e-18	81.3
MsG0680030605.01.T01	MTR_8g092650	32.117	137	93	0	8	144	129	265	4.42e-18	79.0
MsG0680030605.01.T01	MTR_3g095450	28.662	157	102	1	7	163	206	352	6.68e-18	79.7
MsG0680030605.01.T01	MTR_8g092650	32.117	137	93	0	8	144	207	343	9.44e-18	79.3
MsG0680030605.01.T01	MTR_4g094342	33.094	139	93	0	10	148	216	354	4.08e-17	77.4
MsG0680030605.01.T01	MTR_8g006090	35.714	126	79	1	13	136	133	258	5.18e-17	76.3
MsG0680030605.01.T01	MTR_6g081840	32.168	143	96	1	4	146	206	347	5.21e-17	77.0
MsG0680030605.01.T01	MTR_1g074020	40.000	130	72	4	9	136	208	333	7.08e-17	76.6
MsG0680030605.01.T01	MTR_8g006090	35.714	126	79	1	13	136	213	338	8.33e-17	76.6
MsG0680030605.01.T01	MTR_1g074160	34.783	138	85	2	9	141	208	345	1.36e-16	75.9
MsG0680030605.01.T01	MTR_2g065550	33.553	152	95	3	13	160	212	361	1.51e-16	75.9
MsG0680030605.01.T01	MTR_5g069040	31.169	154	102	2	6	155	217	370	2.03e-16	75.9
MsG0680030605.01.T01	MTR_3g019050	33.117	154	99	2	6	155	213	366	2.25e-16	75.5
MsG0680030605.01.T01	MTR_1g051120	38.889	126	72	2	9	131	213	336	2.50e-16	75.1
MsG0680030605.01.T01	MTR_3g095420	28.030	132	95	0	4	135	205	336	3.91e-16	74.7
MsG0680030605.01.T01	MTR_1g073990	41.129	124	72	1	9	131	212	335	4.86e-16	74.3
MsG0680030605.01.T01	MTR_1g074070	37.405	131	74	3	9	136	211	336	8.33e-16	73.9
MsG0680030605.01.T01	MTR_1g030600	34.559	136	86	2	9	143	217	350	1.04e-15	73.6
MsG0680030605.01.T01	MTR_5g034970	38.710	124	63	1	8	131	203	313	1.15e-15	73.2
MsG0680030605.01.T01	MTR_8g006120	34.400	125	80	1	13	135	212	336	1.26e-15	73.6
MsG0680030605.01.T01	MTR_7g111830	29.012	162	97	2	10	153	212	373	1.28e-15	73.6
MsG0680030605.01.T01	MTR_1g030400	32.075	159	96	2	10	156	211	369	1.36e-15	73.2
MsG0680030605.01.T01	MTR_5g068790	33.600	125	83	0	6	130	215	339	1.49e-15	73.2
MsG0680030605.01.T01	MTR_5g089390	30.137	146	99	1	2	144	199	344	2.85e-15	72.4
MsG0680030605.01.T01	MTR_6g036450	32.061	131	86	1	8	135	214	344	3.99e-15	72.0
MsG0680030605.01.T01	MTR_1g070175	32.090	134	88	1	8	141	208	338	5.37e-15	71.6
MsG0680030605.01.T01	MTR_4g094352	33.884	121	80	0	10	130	218	338	5.98e-15	71.6
MsG0680030605.01.T01	MTR_3g112480	29.605	152	105	1	8	157	102	253	9.96e-15	70.1
MsG0680030605.01.T01	MTR_1g068630	33.333	123	81	1	9	130	210	332	1.01e-14	70.9
MsG0680030605.01.T01	MTR_3g112480	29.605	152	105	1	8	157	229	380	1.72e-14	70.1
MsG0680030605.01.T01	MTR_3g112480	29.605	152	105	1	8	157	174	325	1.83e-14	70.1
MsG0680030605.01.T01	MTR_4g129650	29.341	167	108	4	6	163	96	261	5.53e-14	67.8
MsG0680030605.01.T01	MTR_7g100050	30.894	123	84	1	9	130	210	332	6.80e-14	68.6
MsG0680030605.01.T01	MTR_4g129650	29.341	167	108	4	6	163	203	368	8.85e-14	68.2
MsG0680030605.01.T01	MTR_1g023180	30.469	128	89	0	4	131	231	358	9.72e-14	68.2
MsG0680030605.01.T01	MTR_1g038560	28.455	123	86	2	10	131	200	321	1.55e-13	67.4
MsG0680030605.01.T01	MTR_2g016710	28.455	123	88	0	8	130	202	324	1.97e-13	67.0
MsG0680030605.01.T01	MTR_1g114210	30.400	125	85	2	7	130	205	328	3.28e-13	66.2
MsG0680030605.01.T01	MTR_3g095440	30.233	129	90	0	7	135	206	334	1.03e-12	65.1
MsG0680030605.01.T01	MTR_3g072520	32.624	141	93	2	9	147	208	348	5.49e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030605.01.T01	AT3G28050	42.331	163	81	3	7	156	202	364	2.51e-38	134
MsG0680030605.01.T01	AT5G40240	45.255	137	74	1	7	142	217	353	2.91e-36	129
MsG0680030605.01.T01	AT5G40240	45.255	137	74	1	7	142	217	353	2.91e-36	129
MsG0680030605.01.T01	AT5G40240	45.255	137	74	1	7	142	231	367	4.00e-36	129
MsG0680030605.01.T01	AT5G40230	40.667	150	84	1	7	156	218	362	2.89e-33	121
MsG0680030605.01.T01	AT4G15540	44.030	134	75	0	7	140	201	334	5.09e-33	120
MsG0680030605.01.T01	AT4G15540	44.030	134	75	0	7	140	201	334	5.09e-33	120
MsG0680030605.01.T01	AT5G40210	36.842	152	90	3	7	155	191	339	2.20e-29	110
MsG0680030605.01.T01	AT4G08300	39.416	137	80	1	9	142	210	346	1.71e-28	108
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	84	230	6.78e-27	101
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	121	267	1.32e-26	101
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	121	267	1.32e-26	101
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	121	267	1.32e-26	101
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	121	267	1.32e-26	101
MsG0680030605.01.T01	AT3G28130	40.800	125	72	2	7	130	84	207	4.76e-26	99.4
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	202	348	6.09e-26	101
MsG0680030605.01.T01	AT3G28080	35.762	151	92	3	7	156	210	356	6.82e-26	101
MsG0680030605.01.T01	AT3G28070	33.775	151	94	3	7	156	84	229	6.92e-26	99.0
MsG0680030605.01.T01	AT3G28130	40.800	125	72	2	7	130	123	246	1.00e-25	99.4
MsG0680030605.01.T01	AT3G28130	40.800	125	72	2	7	130	123	246	1.00e-25	99.4
MsG0680030605.01.T01	AT3G28070	33.775	151	94	3	7	156	121	266	1.52e-25	99.0
MsG0680030605.01.T01	AT3G28130	40.800	125	72	2	7	130	209	332	4.00e-25	99.4
MsG0680030605.01.T01	AT1G44800	38.583	127	78	0	7	133	205	331	4.53e-25	99.4
MsG0680030605.01.T01	AT3G28070	33.775	151	94	3	7	156	213	358	6.61e-25	99.0
MsG0680030605.01.T01	AT1G21890	38.583	127	78	0	9	135	216	342	2.11e-24	97.8
MsG0680030605.01.T01	AT3G28100	33.333	129	84	2	7	134	210	337	4.43e-22	91.3
MsG0680030605.01.T01	AT2G39510	35.443	158	99	2	9	165	204	359	1.64e-20	87.0
MsG0680030605.01.T01	AT1G70260	34.058	138	91	0	9	146	216	353	3.41e-20	86.3
MsG0680030605.01.T01	AT5G64700	35.200	125	81	0	7	131	209	333	7.23e-19	82.4
MsG0680030605.01.T01	AT2G37450	31.818	154	98	3	9	161	110	257	2.00e-18	80.1
MsG0680030605.01.T01	AT2G37450	31.818	154	98	3	9	161	143	290	2.66e-18	80.1
MsG0680030605.01.T01	AT3G28060	38.835	103	61	2	7	108	53	154	4.44e-18	76.6
MsG0680030605.01.T01	AT2G37450	33.333	138	84	2	9	138	182	319	5.16e-18	79.7
MsG0680030605.01.T01	AT5G07050	35.606	132	84	1	1	131	213	344	1.23e-17	79.3
MsG0680030605.01.T01	AT1G43650	33.333	135	90	0	7	141	198	332	2.01e-17	78.2
MsG0680030605.01.T01	AT1G60050	29.252	147	99	1	9	150	219	365	3.61e-17	77.8
MsG0680030605.01.T01	AT3G28080	42.697	89	48	1	68	156	128	213	4.75e-17	75.5
MsG0680030605.01.T01	AT3G18200	34.028	144	89	1	8	145	98	241	4.99e-17	76.3
MsG0680030605.01.T01	AT2G37460	31.884	138	93	1	9	145	209	346	9.31e-17	76.6
MsG0680030605.01.T01	AT3G18200	34.028	144	89	1	8	145	228	371	9.62e-17	76.6
MsG0680030605.01.T01	AT3G28080	42.697	89	48	1	68	156	217	302	1.18e-16	75.9
MsG0680030605.01.T01	AT2G40900	31.496	127	87	0	9	135	202	328	2.99e-16	75.1
MsG0680030605.01.T01	AT3G56620	32.773	119	80	0	9	127	203	321	4.86e-16	74.7
MsG0680030605.01.T01	AT4G08290	29.078	141	100	0	9	149	211	351	5.30e-16	74.7
MsG0680030605.01.T01	AT5G47470	34.014	147	90	3	9	151	249	392	6.76e-16	74.3
MsG0680030605.01.T01	AT5G47470	34.014	147	90	3	9	151	221	364	8.55e-16	73.9
MsG0680030605.01.T01	AT4G28040	38.168	131	79	2	6	135	192	321	1.47e-15	73.2
MsG0680030605.01.T01	AT4G28040	38.168	131	79	2	6	135	192	321	1.47e-15	73.2
MsG0680030605.01.T01	AT4G28040	38.168	131	79	2	6	135	192	321	1.47e-15	73.2
MsG0680030605.01.T01	AT4G28040	38.168	131	79	2	6	135	192	321	1.47e-15	73.2
MsG0680030605.01.T01	AT4G28040	38.168	131	79	2	6	135	192	321	1.47e-15	73.2
MsG0680030605.01.T01	AT1G68170	30.818	159	102	1	2	152	205	363	5.70e-15	71.6
MsG0680030605.01.T01	AT4G01430	27.273	132	95	1	11	141	73	204	7.07e-15	69.7
MsG0680030605.01.T01	AT1G68170	30.818	159	102	1	2	152	195	353	7.35e-15	71.2
MsG0680030605.01.T01	AT3G30340	29.839	124	87	0	8	131	207	330	8.46e-15	70.9
MsG0680030605.01.T01	AT1G21890	36.634	101	64	0	9	109	216	316	1.00e-14	70.9
MsG0680030605.01.T01	AT5G13670	30.216	139	92	3	7	141	205	342	1.38e-14	70.5
MsG0680030605.01.T01	AT5G47470	33.846	130	80	2	9	134	249	376	1.57e-14	70.5
MsG0680030605.01.T01	AT4G01430	26.277	137	100	1	11	146	210	346	3.45e-14	69.3
MsG0680030605.01.T01	AT1G25270	35.849	106	68	0	26	131	218	323	7.87e-14	68.2
MsG0680030605.01.T01	AT1G75500	29.167	144	99	1	4	147	218	358	8.44e-14	68.2
MsG0680030605.01.T01	AT1G75500	29.167	144	99	1	4	147	218	358	8.44e-14	68.2
MsG0680030605.01.T01	AT4G30420	32.773	119	80	0	12	130	211	329	2.24e-13	67.0
MsG0680030605.01.T01	AT4G30420	32.773	119	80	0	12	130	201	319	2.74e-13	66.6
MsG0680030605.01.T01	AT1G11450	29.008	131	92	1	11	141	122	251	5.04e-13	65.5
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	128	277	6.43e-13	65.1
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	128	277	6.43e-13	65.1
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	170	319	7.27e-13	65.5
MsG0680030605.01.T01	AT1G11450	29.008	131	92	1	11	141	163	292	7.64e-13	65.1
MsG0680030605.01.T01	AT1G01070	29.167	120	85	0	11	130	169	288	7.91e-13	65.1
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	230	379	7.94e-13	65.5
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	230	379	7.94e-13	65.5
MsG0680030605.01.T01	AT1G11450	29.008	131	92	1	11	141	170	299	9.23e-13	65.1
MsG0680030605.01.T01	AT1G11450	29.008	131	92	1	11	141	214	343	9.59e-13	65.1
MsG0680030605.01.T01	AT5G45370	28.758	153	103	3	8	157	206	355	9.64e-13	65.1
MsG0680030605.01.T01	AT1G09380	31.356	118	81	0	10	127	209	326	1.14e-12	65.1
MsG0680030605.01.T01	AT1G01070	29.167	120	85	0	11	130	216	335	1.33e-12	64.7
MsG0680030605.01.T01	AT3G28070	31.068	103	69	2	7	108	213	314	3.16e-12	63.5
MsG0680030605.01.T01	AT4G19185	28.226	124	88	1	8	131	231	353	1.35e-11	62.0
MsG0680030605.01.T01	AT4G19185	28.226	124	88	1	8	131	153	275	1.60e-11	61.6

Find 40 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAAGTGCTTGGAAAAT+AGG	0.235696	6:+5997209	MsG0680030605.01.T01:CDS
GCCTGGGCAATACACTTAAA+AGG	0.240912	6:+5997775	MsG0680030605.01.T01:CDS
AGAGGAAGATGTTGGTAGTT+TGG	0.251289	6:+5998281	MsG0680030605.01.T01:CDS
TTGGTCTTTATGCTGTATTA+TGG	0.276154	6:+5998238	MsG0680030605.01.T01:CDS
AACACTAAAGTTAGTTCATC+TGG	0.290450	6:-5997128	None:intergenic
AAGACCAATTGAGATTATTA+TGG	0.308192	6:-5998223	None:intergenic
TGGTCTTTATGCTGTATTAT+GGG	0.328682	6:+5998239	MsG0680030605.01.T01:CDS
TGCAAATTGTTATCTCCGTT+TGG	0.371860	6:+5997827	MsG0680030605.01.T01:CDS
ACTCATCTCTATTATTTCTT+CGG	0.375116	6:+5997244	MsG0680030605.01.T01:CDS
GCTCTATAGCTTTGCAAAAG+AGG	0.379932	6:-5998327	None:intergenic
TTGAATATAGGCATATTTGT+AGG	0.383051	6:-5998651	None:intergenic
CTGTCTATGTAACCTCATTC+AGG	0.400748	6:+5997800	MsG0680030605.01.T01:CDS
GCTAGTATTTAGTGTATGTT+TGG	0.404500	6:+5996852	None:intergenic
AAAATGTTTAAAAGAATGAA+TGG	0.415749	6:+5998407	MsG0680030605.01.T01:three_prime_UTR
ATTGTTATCTCCGTTTGGTT+GGG	0.429576	6:+5997832	MsG0680030605.01.T01:CDS
AATTGTTATCTCCGTTTGGT+TGG	0.430607	6:+5997831	MsG0680030605.01.T01:CDS
TGATATGATGGTGGCAGTTA+TGG	0.433113	6:-5997159	None:intergenic
CCTGGGCAATACACTTAAAA+GGG	0.446899	6:+5997776	MsG0680030605.01.T01:CDS
CTTGGCGATACTCTTCATCT+TGG	0.450609	6:+5997865	MsG0680030605.01.T01:CDS
GGAGCCATAATAATCTCAAT+TGG	0.466604	6:+5998219	MsG0680030605.01.T01:CDS
CAAAGCAACAGAAGAGATAG+AGG	0.466891	6:+5998263	MsG0680030605.01.T01:CDS
AGAGGTGCATTCTCTATAGA+TGG	0.468724	6:-5998309	None:intergenic
TGGTTGGGCGTTATATTCCT+TGG	0.469000	6:+5997847	MsG0680030605.01.T01:CDS
CAGTTCCTAAAGCAAGTGCT+TGG	0.470511	6:+5997201	MsG0680030605.01.T01:CDS
GCATAATTAGTTAATGACTT+TGG	0.473022	6:-5998707	None:intergenic
TAGGCAATATAGTTTCTGCC+TGG	0.481099	6:+5997758	MsG0680030605.01.T01:CDS
GAAGAGATAGAGGAAGATGT+TGG	0.505673	6:+5998273	MsG0680030605.01.T01:CDS
GAATATAACGCCCAACCAAA+CGG	0.516450	6:-5997842	None:intergenic
GCGATACTCTTCATCTTGGA+AGG	0.527441	6:+5997869	MsG0680030605.01.T01:CDS
TTAGTGTATGTTTGGATACA+CGG	0.527692	6:+5996860	None:intergenic
AGGCAATATAGTTTCTGCCT+GGG	0.551084	6:+5997759	MsG0680030605.01.T01:CDS
GGTCTTTATGCTGTATTATG+GGG	0.556844	6:+5998240	MsG0680030605.01.T01:CDS
AATTTGCAGTGGCCTGAATG+AGG	0.562318	6:-5997812	None:intergenic
AGCTACAACTGTTGATATGA+TGG	0.566150	6:-5997171	None:intergenic
ACACTAAAGTTAGTTCATCT+GGG	0.582689	6:-5997127	None:intergenic
CAAGATGAAGAGTATCGCCA+AGG	0.666505	6:-5997864	None:intergenic
CTGGGCAATACACTTAAAAG+GGG	0.679869	6:+5997777	MsG0680030605.01.T01:CDS
CATTAACTAAATCTGCAACG+TGG	0.702845	6:+5998678	MsG0680030605.01.T01:three_prime_UTR
ACGGAGATAACAATTTGCAG+TGG	0.704316	6:-5997823	None:intergenic
TACAACTGTTGATATGATGG+TGG	0.721627	6:-5997168	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATGTTTAAAAGAATGAA+TGG	+	Chr6:5998407-5998426	MsG0680030605.01.T01:three_prime_UTR	15.0%
!!	AATGATTAATGAATTTAACA+AGG	+	Chr6:5997407-5997426	MsG0680030605.01.T01:intron	15.0%
!!	GAAACAAAAATATACATAAA+TGG	-	Chr6:5998180-5998199	None:intergenic	15.0%
!!!	ATCTATTTTTAAACACAATT+CGG	-	Chr6:5997520-5997539	None:intergenic	15.0%
!!	ATTCAAAATCGCTAAAAAAA+AGG	+	Chr6:5998566-5998585	MsG0680030605.01.T01:three_prime_UTR	20.0%
!!!	AGTTAATGACTTTTGAATAT+AGG	-	Chr6:5998666-5998685	None:intergenic	20.0%
!!!	TTTTATTCAGATTGAAAGAT+TGG	-	Chr6:5998745-5998764	None:intergenic	20.0%
!	AAATTCAACATCAGTTTAGA+TGG	+	Chr6:5997346-5997365	MsG0680030605.01.T01:intron	25.0%
!	AAGACCAATTGAGATTATTA+TGG	-	Chr6:5998226-5998245	None:intergenic	25.0%
!	AATAAACACAAATGCTTAAG+TGG	-	Chr6:5997697-5997716	None:intergenic	25.0%
!	CAGAAGAAAAAATTCATGTT+TGG	-	Chr6:5996994-5997013	None:intergenic	25.0%
!	GAAAAAATTCATGTTTGGAT+TGG	-	Chr6:5996989-5997008	None:intergenic	25.0%
!	GATGGAAATGTTTAAAAACA+TGG	+	Chr6:5998372-5998391	MsG0680030605.01.T01:exon	25.0%
!	GCATAATTAGTTAATGACTT+TGG	-	Chr6:5998710-5998729	None:intergenic	25.0%
!	GTTAACACAGTTTAAATATG+TGG	+	Chr6:5997368-5997387	MsG0680030605.01.T01:intron	25.0%
!	TTAAAAACACCACAGAAAAA+CGG	+	Chr6:5998789-5998808	MsG0680030605.01.T01:three_prime_UTR	25.0%
!	TTAAACAGTTCTGTTAGATT+TGG	-	Chr6:5997995-5998014	None:intergenic	25.0%
!	TTGAATATAGGCATATTTGT+AGG	-	Chr6:5998654-5998673	None:intergenic	25.0%
!!	ACTCATCTCTATTATTTCTT+CGG	+	Chr6:5997244-5997263	MsG0680030605.01.T01:CDS	25.0%
!!	ATTTTTAAACACAATTCGGA+TGG	-	Chr6:5997516-5997535	None:intergenic	25.0%
!!	TTTTTAAACACAATTCGGAT+GGG	-	Chr6:5997515-5997534	None:intergenic	25.0%
!!!	ACTTTTGAAAAGAAACTAGA+TGG	+	Chr6:5998354-5998373	MsG0680030605.01.T01:CDS	25.0%
!!!	ATGACACTTTTTTTTTTCCA+GGG	+	Chr6:5997718-5997737	MsG0680030605.01.T01:intron	25.0%
!!!	GTTATTTTCAGTTTGGTTTT+TGG	-	Chr6:5998023-5998042	None:intergenic	25.0%
!!!	TTTTTTTTCCAGGGAATTTT+CGG	+	Chr6:5997727-5997746	MsG0680030605.01.T01:intron	25.0%
	AAATATGCACTTGAAAACTG+TGG	+	Chr6:5997567-5997586	MsG0680030605.01.T01:intron	30.0%
	AACACTAAAGTTAGTTCATC+TGG	-	Chr6:5997131-5997150	None:intergenic	30.0%
	ACACTAAAGTTAGTTCATCT+GGG	-	Chr6:5997130-5997149	None:intergenic	30.0%
	ACAGTTCAAATATATGACAG+TGG	-	Chr6:5997665-5997684	None:intergenic	30.0%
	AGTTCCTTTATTCATTTCAC+AGG	+	Chr6:5997085-5997104	MsG0680030605.01.T01:intron	30.0%
	CTTTATTAACCACTACTTTC+AGG	+	Chr6:5997480-5997499	MsG0680030605.01.T01:intron	30.0%
	GTTAATAAAGTTCAAATCCG+TGG	-	Chr6:5997470-5997489	None:intergenic	30.0%
	TAAAAACACCACAGAAAAAC+GGG	+	Chr6:5998790-5998809	MsG0680030605.01.T01:three_prime_UTR	30.0%
	TGGTCTTTATGCTGTATTAT+GGG	+	Chr6:5998239-5998258	MsG0680030605.01.T01:CDS	30.0%
	TGTACCTGTGAAATGAATAA+AGG	-	Chr6:5997092-5997111	None:intergenic	30.0%
	TTCAAATATATGACAGTGGA+TGG	-	Chr6:5997661-5997680	None:intergenic	30.0%
!	TCTGCATTTTGCAGTATAAT+TGG	+	Chr6:5998198-5998217	MsG0680030605.01.T01:intron	30.0%
!	TTGGTCTTTATGCTGTATTA+TGG	+	Chr6:5998238-5998257	MsG0680030605.01.T01:CDS	30.0%
!	TTGGTTTGACAAAATCACAA+TGG	+	Chr6:5996883-5996902	MsG0680030605.01.T01:intron	30.0%
!	TTTTAAACACAATTCGGATG+GGG	-	Chr6:5997514-5997533	None:intergenic	30.0%
!!!	GATGACACTTTTTTTTTTCC+AGG	+	Chr6:5997717-5997736	MsG0680030605.01.T01:intron	30.0%
!!!	TTTTTTTCCAGGGAATTTTC+GGG	+	Chr6:5997728-5997747	MsG0680030605.01.T01:intron	30.0%
	AAAGCAAGTGCTTGGAAAAT+AGG	+	Chr6:5997209-5997228	MsG0680030605.01.T01:CDS	35.0%
	AACACAAATGCTTAAGTGGA+TGG	-	Chr6:5997693-5997712	None:intergenic	35.0%
	AATTGTTATCTCCGTTTGGT+TGG	+	Chr6:5997831-5997850	MsG0680030605.01.T01:CDS	35.0%
	AGCTACAACTGTTGATATGA+TGG	-	Chr6:5997174-5997193	None:intergenic	35.0%
	ATGAACTGTCAGTCACATTT+CGG	+	Chr6:5998100-5998119	MsG0680030605.01.T01:intron	35.0%
	ATTGTTATCTCCGTTTGGTT+GGG	+	Chr6:5997832-5997851	MsG0680030605.01.T01:CDS	35.0%
	CATTAACTAAATCTGCAACG+TGG	+	Chr6:5998678-5998697	MsG0680030605.01.T01:three_prime_UTR	35.0%
	CATTGAGTTTGACAAACTCA+CGG	-	Chr6:5996966-5996985	None:intergenic	35.0%
	CCATTGAAAACACTTAACCA+CGG	+	Chr6:5997450-5997469	MsG0680030605.01.T01:intron	35.0%
	CTTCGGCAAAAACTAAACTT+TGG	-	Chr6:5996930-5996949	None:intergenic	35.0%
	GGAGCCATAATAATCTCAAT+TGG	+	Chr6:5998219-5998238	MsG0680030605.01.T01:CDS	35.0%
	GGTCTTTATGCTGTATTATG+GGG	+	Chr6:5998240-5998259	MsG0680030605.01.T01:CDS	35.0%
	TACAACTGTTGATATGATGG+TGG	-	Chr6:5997171-5997190	None:intergenic	35.0%
	TGAAAATAACCGAACTGTGA+TGG	+	Chr6:5998033-5998052	MsG0680030605.01.T01:intron	35.0%
	TGCAAATTGTTATCTCCGTT+TGG	+	Chr6:5997827-5997846	MsG0680030605.01.T01:CDS	35.0%
	TTGGAGCTTGAACAAAATCA+TGG	-	Chr6:5996911-5996930	None:intergenic	35.0%
!	ATTTTCCAAGCACTTGCTTT+AGG	-	Chr6:5997209-5997228	None:intergenic	35.0%
!	GGTTAAGTGTTTTCAATGGA+CGG	-	Chr6:5997449-5997468	None:intergenic	35.0%
!	GTTAACGTCGTTTGAAATTG+TGG	+	Chr6:5997589-5997608	MsG0680030605.01.T01:intron	35.0%
!!	CAGTTCGGTTATTTTCAGTT+TGG	-	Chr6:5998030-5998049	None:intergenic	35.0%
!!	GGAATTTTCGGGAAGTTAAT+AGG	+	Chr6:5997739-5997758	MsG0680030605.01.T01:CDS	35.0%
!!	TTTGATTTCCCGTTTTTCTG+TGG	-	Chr6:5998801-5998820	None:intergenic	35.0%
!!!	TTGAAAGATTGGTTTTGAGC+AGG	-	Chr6:5998734-5998753	None:intergenic	35.0%
	ACGGAGATAACAATTTGCAG+TGG	-	Chr6:5997826-5997845	None:intergenic	40.0%
	ACTCTTCATCTTGGAAGGTA+TGG	+	Chr6:5997874-5997893	MsG0680030605.01.T01:intron	40.0%
	AGGCAATATAGTTTCTGCCT+GGG	+	Chr6:5997759-5997778	MsG0680030605.01.T01:CDS	40.0%
	CAAAGCAACAGAAGAGATAG+AGG	+	Chr6:5998263-5998282	MsG0680030605.01.T01:CDS	40.0%
	CCTGGGCAATACACTTAAAA+GGG	+	Chr6:5997776-5997795	MsG0680030605.01.T01:CDS	40.0%
	CTGGGCAATACACTTAAAAG+GGG	+	Chr6:5997777-5997796	MsG0680030605.01.T01:CDS	40.0%
	CTGTCTATGTAACCTCATTC+AGG	+	Chr6:5997800-5997819	MsG0680030605.01.T01:CDS	40.0%
	GAAGAGATAGAGGAAGATGT+TGG	+	Chr6:5998273-5998292	MsG0680030605.01.T01:CDS	40.0%
	GAATATAACGCCCAACCAAA+CGG	-	Chr6:5997845-5997864	None:intergenic	40.0%
	GATCAAACACCTGAAAGTAG+TGG	-	Chr6:5997492-5997511	None:intergenic	40.0%
	TAGGCAATATAGTTTCTGCC+TGG	+	Chr6:5997758-5997777	MsG0680030605.01.T01:CDS	40.0%
	TTAACTTCCCGAAAATTCCC+TGG	-	Chr6:5997738-5997757	None:intergenic	40.0%
!	AGAGGTGCATTCTCTATAGA+TGG	-	Chr6:5998312-5998331	None:intergenic	40.0%
!	CCGTGGTTAAGTGTTTTCAA+TGG	-	Chr6:5997453-5997472	None:intergenic	40.0%
!	GCTCTATAGCTTTGCAAAAG+AGG	-	Chr6:5998330-5998349	None:intergenic	40.0%
!	TTAACTGAACCGAAGTGCTT+CGG	+	Chr6:5998069-5998088	MsG0680030605.01.T01:intron	40.0%
!!	AGAGGAAGATGTTGGTAGTT+TGG	+	Chr6:5998281-5998300	MsG0680030605.01.T01:CDS	40.0%
!!	TGATATGATGGTGGCAGTTA+TGG	-	Chr6:5997162-5997181	None:intergenic	40.0%
	AATTTGCAGTGGCCTGAATG+AGG	-	Chr6:5997815-5997834	None:intergenic	45.0%
	AGTGGATGATTAATGCGTCG+TGG	+	Chr6:5997624-5997643	MsG0680030605.01.T01:intron	45.0%
	CAGTTCCTAAAGCAAGTGCT+TGG	+	Chr6:5997201-5997220	MsG0680030605.01.T01:CDS	45.0%
	CGACGCATTAATCATCCACT+CGG	-	Chr6:5997624-5997643	None:intergenic	45.0%
	CTTGGCGATACTCTTCATCT+TGG	+	Chr6:5997865-5997884	MsG0680030605.01.T01:CDS	45.0%
	GCCTGGGCAATACACTTAAA+AGG	+	Chr6:5997775-5997794	MsG0680030605.01.T01:CDS	45.0%
	GCGATACTCTTCATCTTGGA+AGG	+	Chr6:5997869-5997888	MsG0680030605.01.T01:CDS	45.0%
	GTGAGCTATCCATCACAGTT+CGG	-	Chr6:5998045-5998064	None:intergenic	45.0%
	TGGTTGGGCGTTATATTCCT+TGG	+	Chr6:5997847-5997866	MsG0680030605.01.T01:CDS	45.0%
	TTGTGGTTAATTGTGCCGAG+TGG	+	Chr6:5997606-5997625	MsG0680030605.01.T01:intron	45.0%
!	TGAACTGAACCGAAGCACTT+CGG	-	Chr6:5998081-5998100	None:intergenic	45.0%
!!	CAAGATGAAGAGTATCGCCA+AGG	-	Chr6:5997867-5997886	None:intergenic	45.0%
!!	CCCTTTTAAGTGTATTGCCC+AGG	-	Chr6:5997779-5997798	None:intergenic	45.0%
	ACGGTGTAACGCGATGACTT+CGG	-	Chr6:5996947-5996966	None:intergenic	50.0%
	CTGTCAGTCACATTTCGGTG+CGG	+	Chr6:5998105-5998124	MsG0680030605.01.T01:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	5996867	5998897	5996867	ID=MsG0680030605.01;Name=MsG0680030605.01
Chr6	mRNA	5996867	5998897	5996867	ID=MsG0680030605.01.T01;Parent=MsG0680030605.01;Name=MsG0680030605.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0.66\|0.75\|1\|1\|1\|4\|511\|166
Chr6	exon	5996867	5996881	5996867	ID=MsG0680030605.01.T01:exon:5873;Parent=MsG0680030605.01.T01
Chr6	exon	5997107	5997265	5997107	ID=MsG0680030605.01.T01:exon:5874;Parent=MsG0680030605.01.T01
Chr6	exon	5997739	5997890	5997739	ID=MsG0680030605.01.T01:exon:5875;Parent=MsG0680030605.01.T01
Chr6	exon	5998212	5998897	5998212	ID=MsG0680030605.01.T01:exon:5876;Parent=MsG0680030605.01.T01
Chr6	CDS	5996867	5996881	5996867	ID=MsG0680030605.01.T01:cds;Parent=MsG0680030605.01.T01
Chr6	CDS	5997107	5997265	5997107	ID=MsG0680030605.01.T01:cds;Parent=MsG0680030605.01.T01
Chr6	CDS	5997739	5997890	5997739	ID=MsG0680030605.01.T01:cds;Parent=MsG0680030605.01.T01
Chr6	CDS	5998212	5998386	5998212	ID=MsG0680030605.01.T01:cds;Parent=MsG0680030605.01.T01
Chr6	three_prime_UTR	5998387	5998897	5998387	ID=MsG0680030605.01.T01:three_prime_utr;Parent=MsG0680030605.01.T01

Gene Sequence

>MsG0680030605.01.T01

ATGTTTGGATACACGGTACATATCTTGAAAGAGTTCCCAGATGAACTAACTTTAGTGTTGTTATACTCCATAACTGCCACCATCATATCAACAGTTGTAGCTTTGTTATCAGTTCCTAAAGCAAGTGCTTGGAAAATAGGACTAAATCTCTCACTCATCTCTATTATTTCTTCGGGAATTTTCGGGAAGTTAATAGGCAATATAGTTTCTGCCTGGGCAATACACTTAAAAGGGGCTGTCTATGTAACCTCATTCAGGCCACTGCAAATTGTTATCTCCGTTTGGTTGGGCGTTATATTCCTTGGCGATACTCTTCATCTTGGAAGTATAATTGGAGCCATAATAATCTCAATTGGTCTTTATGCTGTATTATGGGGCAAAGCAACAGAAGAGATAGAGGAAGATGTTGGTAGTTTGGAGTCACCATCTATAGAGAATGCACCTCTTTTGCAAAGCTATAGAGCAGAAACTTTTGAAAAGAAACTAGATGGAAATGTTTAA

Protein sequence

>MsG0680030605.01.T01

MFGYTVHILKEFPDELTLVLLYSITATIISTVVALLSVPKASAWKIGLNLSLISIISSGIFGKLIGNIVSAWAIHLKGAVYVTSFRPLQIVISVWLGVIFLGDTLHLGSIIGAIIISIGLYAVLWGKATEEIEEDVGSLESPSIENAPLLQSYRAETFEKKLDGNV*