AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043298.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043298.01.T01 MTR_8g432680 94.444 72 4 0 1 72 567 638 9.59e-43 145
MsG0880043298.01.T01 MTR_7g076170 77.778 72 16 0 1 72 562 633 2.49e-32 117
MsG0880043298.01.T01 MTR_0019s0120 59.722 72 29 0 1 72 563 634 1.53e-26 100
MsG0880043298.01.T01 MTR_1g101370 63.077 65 24 0 1 65 554 618 6.55e-25 96.3
MsG0880043298.01.T01 MTR_5g040360 65.152 66 23 0 1 66 536 601 2.18e-24 94.7
MsG0880043298.01.T01 MTR_8g100075 57.971 69 29 0 4 72 560 628 1.40e-22 89.4
MsG0880043298.01.T01 MTR_8g055840 63.492 63 23 0 2 64 538 600 5.48e-22 87.8
MsG0880043298.01.T01 MTR_3g040370 63.077 65 24 0 2 66 535 599 6.09e-22 87.8
MsG0880043298.01.T01 MTR_4g098890 60.656 61 24 0 1 61 540 600 4.64e-21 85.1
MsG0880043298.01.T01 MTR_8g432620 44.444 63 35 0 3 65 539 601 5.94e-14 65.1
MsG0880043298.01.T01 MTR_5g080720 45.902 61 33 0 1 61 577 637 1.29e-12 61.2
MsG0880043298.01.T01 MTR_3g068990 41.935 62 36 0 1 62 605 666 2.77e-12 60.1
MsG0880043298.01.T01 MTR_7g076150 45.161 62 34 0 3 64 383 444 9.20e-12 58.5
MsG0880043298.01.T01 MTR_1g029730 46.032 63 34 0 1 63 596 658 6.06e-11 56.2
MsG0880043298.01.T01 MTR_7g076150 45.161 62 34 0 3 64 526 587 6.79e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043298.01.T01 AT4G00750 69.444 72 22 0 1 72 559 630 8.43e-30 110
MsG0880043298.01.T01 AT2G45750 61.972 71 27 0 2 72 553 623 2.99e-25 97.1
MsG0880043298.01.T01 AT1G33170 62.121 66 25 0 4 69 574 639 3.60e-24 94.0
MsG0880043298.01.T01 AT4G10440 61.765 68 26 0 4 71 556 623 1.48e-23 92.4
MsG0880043298.01.T01 AT1G26850 59.155 71 29 0 2 72 546 616 6.80e-23 90.5
MsG0880043298.01.T01 AT1G26850 59.155 71 29 0 2 72 546 616 6.80e-23 90.5
MsG0880043298.01.T01 AT4G18030 63.636 66 24 0 1 66 540 605 1.54e-22 89.4
MsG0880043298.01.T01 AT2G39750 46.774 62 33 0 1 62 629 690 6.19e-14 65.1
MsG0880043298.01.T01 AT4G00740 43.077 65 37 0 3 67 532 596 8.50e-14 64.7
MsG0880043298.01.T01 AT4G00740 43.077 65 37 0 3 67 532 596 8.50e-14 64.7
MsG0880043298.01.T01 AT5G06050 39.683 63 38 0 1 63 601 663 3.25e-13 62.8
MsG0880043298.01.T01 AT1G77260 40.323 62 37 0 1 62 593 654 6.64e-12 59.3
MsG0880043298.01.T01 AT2G43200 46.429 56 30 0 3 58 550 605 1.39e-11 58.2
MsG0880043298.01.T01 AT2G43200 46.429 56 30 0 3 58 550 605 1.39e-11 58.2
MsG0880043298.01.T01 AT1G31850 45.312 64 34 1 1 64 534 596 5.05e-11 56.6
MsG0880043298.01.T01 AT1G31850 45.312 64 34 1 1 64 534 596 5.05e-11 56.6
MsG0880043298.01.T01 AT1G31850 45.312 64 34 1 1 64 534 596 5.05e-11 56.6
MsG0880043298.01.T01 AT1G31850 45.312 64 34 1 1 64 534 596 5.05e-11 56.6

Find 9 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCAAAATTACACCACCTTT+TGG 0.268044 8:+22181173 None:intergenic
TTTGATAAGGTCCTTCTTCA+TGG 0.322080 8:+22181078 None:intergenic
TTGTAGCAGTCAAGCAATAC+TGG 0.370223 8:-22181043 MsG0880043298.01.T01:CDS
ACATTCTTCTAGAGATGGAC+CGG 0.518254 8:-22181205 MsG0880043298.01.T01:CDS
GGAAAACATTCTTCTAGAGA+TGG 0.550144 8:-22181210 MsG0880043298.01.T01:CDS
AGAATTAGAGACCATGAAGA+AGG 0.559942 8:-22181089 MsG0880043298.01.T01:CDS
GCATTGCAGATGAAATGCAG+TGG 0.646503 8:-22181118 MsG0880043298.01.T01:CDS
CATTGCAGATGAAATGCAGT+GGG 0.662717 8:-22181117 MsG0880043298.01.T01:CDS
CAATGTAGATGTGTTGACGA+AGG 0.740038 8:-22181147 MsG0880043298.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTATCTTTTCACTTTGATA+AGG + Chr8:22181166-22181185 None:intergenic 25.0%
AGAATTAGAGACCATGAAGA+AGG - Chr8:22181139-22181158 MsG0880043298.01.T01:CDS 35.0%
TTTGATAAGGTCCTTCTTCA+TGG + Chr8:22181153-22181172 None:intergenic 35.0%
! GGAAAACATTCTTCTAGAGA+TGG - Chr8:22181018-22181037 MsG0880043298.01.T01:CDS 35.0%
CATTGCAGATGAAATGCAGT+GGG - Chr8:22181111-22181130 MsG0880043298.01.T01:CDS 40.0%
TTGTAGCAGTCAAGCAATAC+TGG - Chr8:22181185-22181204 MsG0880043298.01.T01:CDS 40.0%
! ACATTCTTCTAGAGATGGAC+CGG - Chr8:22181023-22181042 MsG0880043298.01.T01:CDS 40.0%
! CAATGTAGATGTGTTGACGA+AGG - Chr8:22181081-22181100 MsG0880043298.01.T01:CDS 40.0%
! CGCAAAATTACACCACCTTT+TGG + Chr8:22181058-22181077 None:intergenic 40.0%
GCATTGCAGATGAAATGCAG+TGG - Chr8:22181110-22181129 MsG0880043298.01.T01:CDS 45.0%
! TAGAGATGGACCGGATTTTG+CGG - Chr8:22181032-22181051 MsG0880043298.01.T01:CDS 45.0%
! CACCTTTTGGCCGCAAAATC+CGG + Chr8:22181045-22181064 None:intergenic 50.0%
! CGGATTTTGCGGCCAAAAGG+TGG - Chr8:22181043-22181062 MsG0880043298.01.T01:CDS 55.0%
! GACCGGATTTTGCGGCCAAA+AGG - Chr8:22181040-22181059 MsG0880043298.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 22181016 22181234 22181016 ID=MsG0880043298.01;Name=MsG0880043298.01
Chr8 mRNA 22181016 22181234 22181016 ID=MsG0880043298.01.T01;Parent=MsG0880043298.01;Name=MsG0880043298.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|72
Chr8 exon 22181016 22181234 22181016 ID=MsG0880043298.01.T01:exon:5893;Parent=MsG0880043298.01.T01
Chr8 CDS 22181016 22181234 22181016 ID=MsG0880043298.01.T01:cds;Parent=MsG0880043298.01.T01
Gene Sequence

>MsG0880043298.01.T01

ATGGAAAACATTCTTCTAGAGATGGACCGGATTTTGCGGCCAAAAGGTGGTGTAATTTTGCGCGACAATGTAGATGTGTTGACGAAGGTGAAGAGCATTGCAGATGAAATGCAGTGGGATGCAAGAATTAGAGACCATGAAGAAGGACCTTATCAAAGTGAAAAGATACTTGTAGCAGTCAAGCAATACTGGACAGCTCCACCAACAGAGCAAAACTAA

Protein sequence

>MsG0880043298.01.T01

MENILLEMDRILRPKGGVILRDNVDVLTKVKSIADEMQWDARIRDHEEGPYQSEKILVAVKQYWTAPPTEQN*