AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030862.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030862.01.T01 MTR_6g013170 93.143 175 12 0 1 175 1 175 2.47e-120 337
MsG0680030862.01.T01 MTR_5g088000 64.762 105 36 1 34 137 24 128 2.00e-45 146
MsG0680030862.01.T01 MTR_3g117110 55.462 119 52 1 28 145 27 145 8.12e-44 143
MsG0680030862.01.T01 MTR_5g088180 52.800 125 57 2 31 153 29 153 9.36e-44 143
MsG0680030862.01.T01 MTR_5g088170 52.000 125 58 2 31 153 16 140 1.56e-42 139
MsG0680030862.01.T01 MTR_5g087970 55.462 119 47 3 25 137 23 141 7.53e-37 125
MsG0680030862.01.T01 MTR_6g013420 42.424 165 86 4 9 167 17 178 9.09e-33 115
MsG0680030862.01.T01 MTR_6g013200 41.538 130 72 2 4 132 11 137 1.44e-26 99.4
MsG0680030862.01.T01 MTR_4g007250 34.615 130 74 2 31 150 29 157 6.20e-22 87.4
MsG0680030862.01.T01 MTR_6g080660 39.796 98 56 3 31 127 28 123 1.48e-14 68.9
MsG0680030862.01.T01 MTR_5g006040 27.381 168 107 5 1 162 4 162 1.93e-14 68.2
MsG0680030862.01.T01 MTR_2g088990 36.434 129 70 7 31 152 25 148 1.52e-13 66.6
MsG0680030862.01.T01 MTR_1g090420 30.370 135 78 5 33 152 30 163 2.63e-13 65.9
MsG0680030862.01.T01 MTR_6g023760 37.113 97 59 2 31 126 26 121 2.71e-13 65.1
MsG0680030862.01.T01 MTR_8g086360 34.783 138 76 6 9 136 1 134 2.99e-12 62.4
MsG0680030862.01.T01 MTR_1g104800 31.000 100 65 3 30 126 24 122 6.14e-12 61.6
MsG0680030862.01.T01 MTR_8g007020 30.864 162 92 5 33 175 26 186 6.61e-12 61.2
MsG0680030862.01.T01 MTR_7g086140 31.933 119 70 4 1 116 1 111 7.29e-12 60.8
MsG0680030862.01.T01 MTR_7g086190 31.933 119 70 4 1 116 1 111 7.29e-12 60.8
MsG0680030862.01.T01 MTR_8g463180 34.545 110 68 3 33 139 26 134 1.01e-11 62.4
MsG0680030862.01.T01 MTR_3g089005 28.696 115 79 2 12 126 14 125 1.35e-11 59.3
MsG0680030862.01.T01 MTR_7g086160 31.933 119 71 4 1 116 1 112 2.74e-11 59.3
MsG0680030862.01.T01 MTR_3g099980 33.898 118 72 4 16 128 8 124 4.93e-11 59.7
MsG0680030862.01.T01 MTR_8g007035 40.206 97 53 4 33 126 26 120 5.44e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030862.01.T01 AT2G27035 46.565 131 65 2 22 151 19 145 6.52e-39 130
MsG0680030862.01.T01 AT5G15350 46.099 141 70 3 7 146 6 141 1.12e-34 120
MsG0680030862.01.T01 AT4G12880 47.967 123 62 2 6 127 5 126 1.48e-31 111
MsG0680030862.01.T01 AT3G01070 47.059 102 52 2 29 128 25 126 2.24e-19 80.9
MsG0680030862.01.T01 AT3G27200 34.146 123 75 3 5 126 4 121 1.70e-15 70.9
MsG0680030862.01.T01 AT2G32300 32.540 126 79 3 31 151 26 150 2.33e-15 72.0
MsG0680030862.01.T01 AT4G12880 52.174 69 32 1 60 127 20 88 3.63e-15 68.2
MsG0680030862.01.T01 AT2G32300 32.540 126 79 3 31 151 56 180 4.96e-15 71.2
MsG0680030862.01.T01 AT3G17675 31.034 116 78 2 12 126 13 127 2.62e-13 63.9
MsG0680030862.01.T01 AT2G26720 34.711 121 70 5 23 136 22 140 2.83e-13 65.5
MsG0680030862.01.T01 AT2G31050 39.423 104 56 4 27 126 25 125 3.70e-13 65.1
MsG0680030862.01.T01 AT5G26330 29.508 122 81 4 9 126 1 121 2.09e-12 62.8

Find 42 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATGGTGAGTGTGGTTT+TGG 0.224601 6:-10601951 MsG0680030862.01.T01:CDS
CCATCAATGTATAAATGCTT+TGG 0.298696 6:-10600167 MsG0680030862.01.T01:CDS
CCAAAGCATTTATACATTGA+TGG 0.310772 6:+10600167 None:intergenic
AGAATGTCAACTACACTAAA+TGG 0.326899 6:-10601850 MsG0680030862.01.T01:CDS
CATCAATGTATAAATGCTTT+GGG 0.352459 6:-10600166 MsG0680030862.01.T01:CDS
GGTGGAGGTTACTGCTTTCA+TGG 0.363773 6:-10600284 MsG0680030862.01.T01:CDS
GACATTCTGATTCCAACCTT+TGG 0.366517 6:+10601865 None:intergenic
CCTCCCACCTTATGAAGCTT+TGG 0.418516 6:+10601890 None:intergenic
TCTTGTCAAGTTTCTTATGA+AGG 0.439117 6:+10600361 None:intergenic
ATTGTGGCCAATGGTGGTCA+TGG 0.472414 6:-10601918 MsG0680030862.01.T01:CDS
CAAAAGATACTACAACGTAT+TGG 0.479223 6:-10600423 MsG0680030862.01.T01:CDS
AAATGTTGTTTATGTGAGCT+TGG 0.487197 6:-10600132 MsG0680030862.01.T01:CDS
GGACCTCCCATGACCACCAT+TGG 0.487403 6:+10601911 None:intergenic
TTGTGGCCAATGGTGGTCAT+GGG 0.494982 6:-10601917 MsG0680030862.01.T01:CDS
TCGCGATGTGGTTCAATTGA+CGG 0.504641 6:-10600333 MsG0680030862.01.T01:CDS
TACTACTTTATCACCGGTGG+AGG 0.525846 6:-10600299 MsG0680030862.01.T01:CDS
GGGAGGTCCAAAGCTTCATA+AGG 0.541271 6:-10601897 MsG0680030862.01.T01:CDS
AGTGTTGTCATTGTGGCCAA+TGG 0.543550 6:-10601927 MsG0680030862.01.T01:CDS
TTCATTGTATCAGAAAGAGA+TGG 0.552490 6:+10600212 None:intergenic
GAAAGAGATGGAGATGGTGC+AGG 0.556485 6:+10600224 None:intergenic
AGCATGTCTATGTTGGTGAT+TGG 0.563002 6:-10601817 MsG0680030862.01.T01:CDS
TTGATGTTCTTGAACGTCCA+CGG 0.563672 6:+10600250 None:intergenic
TCTCAAGAGCATGTCTATGT+TGG 0.570965 6:-10601824 MsG0680030862.01.T01:CDS
TGGCCAATGGTGGTCATGGG+AGG 0.572145 6:-10601914 MsG0680030862.01.T01:CDS
CATAAGGTGGGAGGATCCAA+AGG 0.579599 6:-10601881 MsG0680030862.01.T01:CDS
GTTGTCATTGTGGCCAATGG+TGG 0.582644 6:-10601924 MsG0680030862.01.T01:CDS
ATAAGAAACTTGACAAGAGG+TGG 0.589525 6:-10600356 MsG0680030862.01.T01:CDS
AGGTGGGAGGATCCAAAGGT+TGG 0.592986 6:-10601877 MsG0680030862.01.T01:CDS
ATGAAAGCAGTAACCTCCAC+CGG 0.596550 6:+10600286 None:intergenic
TTCATAAGAAACTTGACAAG+AGG 0.597109 6:-10600359 MsG0680030862.01.T01:CDS
AGGTCCAAAGCTTCATAAGG+TGG 0.599406 6:-10601894 MsG0680030862.01.T01:CDS
AAGACATACTACTTTATCAC+CGG 0.609394 6:-10600305 MsG0680030862.01.T01:CDS
ACATACTACTTTATCACCGG+TGG 0.610826 6:-10600302 MsG0680030862.01.T01:CDS
GTATTGGAAGTGAACAAGAC+AGG 0.615002 6:-10600407 MsG0680030862.01.T01:CDS
TCTGATACAATGAAATCTAG+TGG 0.617662 6:-10600203 MsG0680030862.01.T01:CDS
TCATGGAATGAAAGTTGCCG+TGG 0.625731 6:-10600267 MsG0680030862.01.T01:CDS
GACAAGAGGTGGTCGCGATG+TGG 0.637295 6:-10600345 MsG0680030862.01.T01:CDS
GGTCCAAAGCTTCATAAGGT+GGG 0.645031 6:-10601893 MsG0680030862.01.T01:CDS
GTATCAGAAAGAGATGGAGA+TGG 0.657311 6:+10600218 None:intergenic
CCAAAGCTTCATAAGGTGGG+AGG 0.659873 6:-10601890 MsG0680030862.01.T01:CDS
GATACAATGAAATCTAGTGG+TGG 0.676277 6:-10600200 MsG0680030862.01.T01:CDS
CGATGTGGTTCAATTGACGG+AGG 0.705107 6:-10600330 MsG0680030862.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAACATTTTAATTTTAATT+TGG - Chr6:10600562-10600581 MsG0680030862.01.T01:intron 10.0%
!! AAAATGATTAAAAAACATGA+TGG + Chr6:10600604-10600623 None:intergenic 15.0%
!! AAGTTATATCTACTATAATA+TGG + Chr6:10601489-10601508 None:intergenic 15.0%
!! AATAAAAGAGAAAAAGTTAA+AGG - Chr6:10601212-10601231 MsG0680030862.01.T01:intron 15.0%
!! AATTAACTATCATATAATGT+TGG - Chr6:10600627-10600646 MsG0680030862.01.T01:intron 15.0%
!!! TTATGAACTATGATAATTTT+TGG - Chr6:10600686-10600705 MsG0680030862.01.T01:intron 15.0%
!! ACAAATCAATAAATGAGAAA+AGG + Chr6:10600943-10600962 None:intergenic 20.0%
!! ACAATGTTAAAGTTTGAAAA+CGG + Chr6:10600994-10601013 None:intergenic 20.0%
!!! AAATTCAAGAGTTATTTTGA+TGG - Chr6:10600117-10600136 MsG0680030862.01.T01:CDS 20.0%
!!! ATAATGAAGTTTTTTTTACG+TGG - Chr6:10601144-10601163 MsG0680030862.01.T01:intron 20.0%
!!! CTTTTGTTTTTACAAACTTT+GGG + Chr6:10600849-10600868 None:intergenic 20.0%
!!! TAATGAAGTTTTTTTTACGT+GGG - Chr6:10601145-10601164 MsG0680030862.01.T01:intron 20.0%
! AAGTTTGTAAAAACAAAAGG+TGG - Chr6:10600850-10600869 MsG0680030862.01.T01:intron 25.0%
! ATATTTCTCGTAATTATCAC+TGG - Chr6:10600726-10600745 MsG0680030862.01.T01:intron 25.0%
! ATCGAGAAATAAACAATTGT+TGG + Chr6:10600900-10600919 None:intergenic 25.0%
! ATGCTTAGCAAAATAATTAC+TGG - Chr6:10601612-10601631 MsG0680030862.01.T01:intron 25.0%
! CATCAATGTATAAATGCTTT+GGG - Chr6:10601917-10601936 MsG0680030862.01.T01:CDS 25.0%
! CCAAAGTTTGTAAAAACAAA+AGG - Chr6:10600847-10600866 MsG0680030862.01.T01:intron 25.0%
! CCAATATGGAATTTCATAAA+AGG - Chr6:10601406-10601425 MsG0680030862.01.T01:intron 25.0%
! GACAAAATTGAAATCCAATA+TGG - Chr6:10601392-10601411 MsG0680030862.01.T01:intron 25.0%
! TCTTATTCTAACACTAGAAA+AGG - Chr6:10601570-10601589 MsG0680030862.01.T01:intron 25.0%
!! CCTTTTATGAAATTCCATAT+TGG + Chr6:10601409-10601428 None:intergenic 25.0%
!! TAGCAAAATAATTACTGGTT+TGG - Chr6:10601617-10601636 MsG0680030862.01.T01:intron 25.0%
!!! ATCGTTTTGATTATTGATTG+TGG - Chr6:10600482-10600501 MsG0680030862.01.T01:intron 25.0%
!!! CCTTTTGTTTTTACAAACTT+TGG + Chr6:10600850-10600869 None:intergenic 25.0%
!!! GATTCATTGCAAACTTTTTT+TGG + Chr6:10601336-10601355 None:intergenic 25.0%
AAGACATACTACTTTATCAC+CGG - Chr6:10601778-10601797 MsG0680030862.01.T01:intron 30.0%
AGAATGTCAACTACACTAAA+TGG - Chr6:10600233-10600252 MsG0680030862.01.T01:CDS 30.0%
ATTACCCTTATGACTGTAAT+GGG + Chr6:10601195-10601214 None:intergenic 30.0%
CAAAAGATACTACAACGTAT+TGG - Chr6:10601660-10601679 MsG0680030862.01.T01:intron 30.0%
CCATCAATGTATAAATGCTT+TGG - Chr6:10601916-10601935 MsG0680030862.01.T01:CDS 30.0%
CTCAATTTCTGCTTAGAATT+TGG - Chr6:10601090-10601109 MsG0680030862.01.T01:intron 30.0%
TATTACCCTTATGACTGTAA+TGG + Chr6:10601196-10601215 None:intergenic 30.0%
TCTGATACAATGAAATCTAG+TGG - Chr6:10601880-10601899 MsG0680030862.01.T01:CDS 30.0%
TCTTGTCAAGTTTCTTATGA+AGG + Chr6:10601725-10601744 None:intergenic 30.0%
TTCATAAGAAACTTGACAAG+AGG - Chr6:10601724-10601743 MsG0680030862.01.T01:intron 30.0%
TTCATTGTATCAGAAAGAGA+TGG + Chr6:10601874-10601893 None:intergenic 30.0%
! AAATGTTGTTTATGTGAGCT+TGG - Chr6:10601951-10601970 MsG0680030862.01.T01:CDS 30.0%
! ATTGTTGGCTAGTAATTGTT+TGG + Chr6:10600885-10600904 None:intergenic 30.0%
! ATTTCAATTTTGTCTCCAAC+TGG + Chr6:10601386-10601405 None:intergenic 30.0%
! CCAAAGCATTTATACATTGA+TGG + Chr6:10601919-10601938 None:intergenic 30.0%
! TTTCAATTTTGTCTCCAACT+GGG + Chr6:10601385-10601404 None:intergenic 30.0%
!! AAATCGTACGTTTTGAATAG+AGG + Chr6:10601290-10601309 None:intergenic 30.0%
AATTAACAAACATCCCCAGT+TGG - Chr6:10601368-10601387 MsG0680030862.01.T01:intron 35.0%
ATAAGAAACTTGACAAGAGG+TGG - Chr6:10601727-10601746 MsG0680030862.01.T01:intron 35.0%
CGATAAACATGAAGATCGAA+TGG + Chr6:10600466-10600485 None:intergenic 35.0%
GATACAATGAAATCTAGTGG+TGG - Chr6:10601883-10601902 MsG0680030862.01.T01:CDS 35.0%
! AACTGGGGATGTTTGTTAAT+TGG + Chr6:10601369-10601388 None:intergenic 35.0%
! TTCAATTTTGTCTCCAACTG+GGG + Chr6:10601384-10601403 None:intergenic 35.0%
!!! AGTTATTTTGATGGTGAGTG+TGG - Chr6:10600126-10600145 MsG0680030862.01.T01:CDS 35.0%
!!! GAAGTTTTTTTTACGTGGGT+TGG - Chr6:10601149-10601168 MsG0680030862.01.T01:intron 35.0%
!!! TATGTGAGCTTGGTTTTAGT+GGG - Chr6:10601961-10601980 MsG0680030862.01.T01:CDS 35.0%
!!! TTATGTGAGCTTGGTTTTAG+TGG - Chr6:10601960-10601979 MsG0680030862.01.T01:CDS 35.0%
ACATACTACTTTATCACCGG+TGG - Chr6:10601781-10601800 MsG0680030862.01.T01:intron 40.0%
GACATTCTGATTCCAACCTT+TGG + Chr6:10600221-10600240 None:intergenic 40.0%
GTATCAGAAAGAGATGGAGA+TGG + Chr6:10601868-10601887 None:intergenic 40.0%
GTATTGGAAGTGAACAAGAC+AGG - Chr6:10601676-10601695 MsG0680030862.01.T01:intron 40.0%
TTGATGTTCTTGAACGTCCA+CGG + Chr6:10601836-10601855 None:intergenic 40.0%
! TCTCAAGAGCATGTCTATGT+TGG - Chr6:10600259-10600278 MsG0680030862.01.T01:CDS 40.0%
!! AGCATGTCTATGTTGGTGAT+TGG - Chr6:10600266-10600285 MsG0680030862.01.T01:CDS 40.0%
!!! ATGTGAGCTTGGTTTTAGTG+GGG - Chr6:10601962-10601981 MsG0680030862.01.T01:CDS 40.0%
!!! TTGATGGTGAGTGTGGTTTT+GGG - Chr6:10600133-10600152 MsG0680030862.01.T01:CDS 40.0%
!!! TTTGATGGTGAGTGTGGTTT+TGG - Chr6:10600132-10600151 MsG0680030862.01.T01:CDS 40.0%
!!! TTTTACGTGGGTTGGAGTAT+TGG - Chr6:10601157-10601176 MsG0680030862.01.T01:intron 40.0%
!!! TTTTGGGAGTGTTGTCATTG+TGG - Chr6:10600149-10600168 MsG0680030862.01.T01:CDS 40.0%
AGGTCCAAAGCTTCATAAGG+TGG - Chr6:10600189-10600208 MsG0680030862.01.T01:CDS 45.0%
AGTGTTGTCATTGTGGCCAA+TGG - Chr6:10600156-10600175 MsG0680030862.01.T01:CDS 45.0%
ATGAAAGCAGTAACCTCCAC+CGG + Chr6:10601800-10601819 None:intergenic 45.0%
GCCACCCATTACAGTCATAA+GGG - Chr6:10601188-10601207 MsG0680030862.01.T01:intron 45.0%
GGTCCAAAGCTTCATAAGGT+GGG - Chr6:10600190-10600209 MsG0680030862.01.T01:CDS 45.0%
TACTACTTTATCACCGGTGG+AGG - Chr6:10601784-10601803 MsG0680030862.01.T01:intron 45.0%
TCATGGAATGAAAGTTGCCG+TGG - Chr6:10601816-10601835 MsG0680030862.01.T01:CDS 45.0%
TCGCGATGTGGTTCAATTGA+CGG - Chr6:10601750-10601769 MsG0680030862.01.T01:intron 45.0%
TGCCACCCATTACAGTCATA+AGG - Chr6:10601187-10601206 MsG0680030862.01.T01:intron 45.0%
! ACCCTTATGACTGTAATGGG+TGG + Chr6:10601192-10601211 None:intergenic 45.0%
!! ACTGTAATGGGTGGCATACT+TGG + Chr6:10601183-10601202 None:intergenic 45.0%
ATTGTGGCCAATGGTGGTCA+TGG - Chr6:10600165-10600184 MsG0680030862.01.T01:CDS 50.0%
CATAAGGTGGGAGGATCCAA+AGG - Chr6:10600202-10600221 MsG0680030862.01.T01:CDS 50.0%
CCAAAGCTTCATAAGGTGGG+AGG - Chr6:10600193-10600212 MsG0680030862.01.T01:CDS 50.0%
CCTCCCACCTTATGAAGCTT+TGG + Chr6:10600196-10600215 None:intergenic 50.0%
CGATGTGGTTCAATTGACGG+AGG - Chr6:10601753-10601772 MsG0680030862.01.T01:intron 50.0%
GAAAGAGATGGAGATGGTGC+AGG + Chr6:10601862-10601881 None:intergenic 50.0%
GCTCTCAACGCGTTTCTTTC+AGG - Chr6:10600779-10600798 MsG0680030862.01.T01:intron 50.0%
GGGAGGTCCAAAGCTTCATA+AGG - Chr6:10600186-10600205 MsG0680030862.01.T01:CDS 50.0%
GGTGGAGGTTACTGCTTTCA+TGG - Chr6:10601799-10601818 MsG0680030862.01.T01:intron 50.0%
GTTGTCATTGTGGCCAATGG+TGG - Chr6:10600159-10600178 MsG0680030862.01.T01:CDS 50.0%
TTGTGGCCAATGGTGGTCAT+GGG - Chr6:10600166-10600185 MsG0680030862.01.T01:CDS 50.0%
AGGTGGGAGGATCCAAAGGT+TGG - Chr6:10600206-10600225 MsG0680030862.01.T01:CDS 55.0%
GACAAGAGGTGGTCGCGATG+TGG - Chr6:10601738-10601757 MsG0680030862.01.T01:intron 60.0%
GGACCTCCCATGACCACCAT+TGG + Chr6:10600175-10600194 None:intergenic 60.0%
TGGCCAATGGTGGTCATGGG+AGG - Chr6:10600169-10600188 MsG0680030862.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 10600112 10601993 10600112 ID=MsG0680030862.01;Name=MsG0680030862.01
Chr6 mRNA 10600112 10601993 10600112 ID=MsG0680030862.01.T01;Parent=MsG0680030862.01;Name=MsG0680030862.01.T01;_AED=0.04;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|175
Chr6 exon 10601813 10601993 10601813 ID=MsG0680030862.01.T01:exon:8090;Parent=MsG0680030862.01.T01
Chr6 exon 10600112 10600458 10600112 ID=MsG0680030862.01.T01:exon:8089;Parent=MsG0680030862.01.T01
Chr6 CDS 10601813 10601993 10601813 ID=MsG0680030862.01.T01:cds;Parent=MsG0680030862.01.T01
Chr6 CDS 10600112 10600458 10600112 ID=MsG0680030862.01.T01:cds;Parent=MsG0680030862.01.T01
Gene Sequence

>MsG0680030862.01.T01

ATGGAAAATTCAAGAGTTATTTTGATGGTGAGTGTGGTTTTGGGAGTGTTGTCATTGTGGCCAATGGTGGTCATGGGAGGTCCAAAGCTTCATAAGGTGGGAGGATCCAAAGGTTGGAATCAGAATGTCAACTACACTAAATGGTCTTCTCAAGAGCATGTCTATGTTGGTGATTGGCTGAAATTTGTCTTTAACAAAAGATACTACAACGTATTGGAAGTGAACAAGACAGGCTATGAATATTGCATTGACATGACCTTCATAAGAAACTTGACAAGAGGTGGTCGCGATGTGGTTCAATTGACGGAGGCAAAGACATACTACTTTATCACCGGTGGAGGTTACTGCTTTCATGGAATGAAAGTTGCCGTGGACGTTCAAGAACATCAAACACCTGCACCATCTCCATCTCTTTCTGATACAATGAAATCTAGTGGTGGTTCTATTTTGCCATCAATGTATAAATGCTTTGGGATTATTGTTGCAAATGTTGTTTATGTGAGCTTGGTTTTAGTGGGGATTTTGTAA

Protein sequence

>MsG0680030862.01.T01

MENSRVILMVSVVLGVLSLWPMVVMGGPKLHKVGGSKGWNQNVNYTKWSSQEHVYVGDWLKFVFNKRYYNVLEVNKTGYEYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDVQEHQTPAPSPSLSDTMKSSGGSILPSMYKCFGIIVANVVYVSLVLVGIL*