AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880042734.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042734.01.T01 MTR_8g020770 95.283 212 10 0 45 256 1 212 7.03e-147 409
MsG0880042734.01.T01 MTR_8g020750 66.667 213 69 2 45 256 1 212 5.22e-96 280
MsG0880042734.01.T01 MTR_4g010340 62.559 211 72 5 47 256 1 205 2.80e-83 248
MsG0880042734.01.T01 MTR_8g020760 57.746 213 87 3 45 256 1 211 1.86e-78 236
MsG0880042734.01.T01 MTR_8g020630 53.774 212 95 2 45 256 1 209 2.31e-78 236
MsG0880042734.01.T01 MTR_8g020650 57.143 189 79 2 68 256 23 209 4.37e-74 224
MsG0880042734.01.T01 MTR_2g044040 52.358 212 98 3 45 256 1 209 1.63e-73 223
MsG0880042734.01.T01 MTR_0284s0010 52.830 212 97 2 45 256 1 209 1.78e-73 223
MsG0880042734.01.T01 MTR_2g036120 53.081 211 96 3 45 255 1 208 1.61e-71 218
MsG0880042734.01.T01 MTR_7g011340 57.143 189 79 1 68 256 23 209 1.89e-71 218
MsG0880042734.01.T01 MTR_3g019710 51.887 212 99 3 45 256 1 209 5.72e-70 214
MsG0880042734.01.T01 MTR_3g073170 52.093 215 95 5 45 256 1 210 3.27e-69 212
MsG0880042734.01.T01 MTR_2g019780 52.535 217 93 5 45 256 1 212 3.72e-69 212
MsG0880042734.01.T01 MTR_8g020670 50.239 209 102 2 48 256 3 209 1.06e-68 211
MsG0880042734.01.T01 MTR_2g045100 51.456 206 98 2 51 256 6 209 3.95e-68 209
MsG0880042734.01.T01 MTR_5g031460 49.528 212 104 3 45 256 1 209 1.12e-67 208
MsG0880042734.01.T01 MTR_8g020763 55.191 183 81 1 74 256 9 190 1.96e-67 207
MsG0880042734.01.T01 MTR_3g019650 50.943 212 100 3 45 256 1 208 2.41e-67 207
MsG0880042734.01.T01 MTR_3g019680 51.415 212 99 3 45 256 1 208 3.81e-67 207
MsG0880042734.01.T01 MTR_8g020610 50.242 207 99 3 51 256 4 207 4.75e-64 199
MsG0880042734.01.T01 MTR_2g041570 46.009 213 111 3 45 256 1 210 7.91e-63 196
MsG0880042734.01.T01 MTR_2g041580 45.540 213 112 3 45 256 1 210 6.38e-62 194
MsG0880042734.01.T01 MTR_8g020590 48.341 211 105 4 47 256 1 208 1.18e-60 190
MsG0880042734.01.T01 MTR_2g041550 46.479 213 110 3 45 256 1 210 1.93e-60 190
MsG0880042734.01.T01 MTR_3g019810 46.377 207 102 3 44 250 106 303 3.42e-51 169
MsG0880042734.01.T01 MTR_4g027415 55.738 122 52 1 68 189 23 142 2.24e-40 136
MsG0880042734.01.T01 MTR_6g005330 42.135 178 90 4 47 214 1 175 3.51e-30 112
MsG0880042734.01.T01 MTR_6g005380 41.899 179 90 4 47 214 1 176 5.29e-30 112
MsG0880042734.01.T01 MTR_7g111240 42.767 159 86 4 68 222 25 182 5.66e-30 112
MsG0880042734.01.T01 MTR_6g005310 41.989 181 86 5 47 214 1 175 7.07e-30 112
MsG0880042734.01.T01 MTR_6g005360 41.989 181 86 5 47 214 1 175 8.37e-30 111
MsG0880042734.01.T01 MTR_2g030855 37.245 196 113 4 60 252 19 207 9.66e-30 111
MsG0880042734.01.T01 MTR_1g085500 41.317 167 92 4 51 213 9 173 1.34e-29 111
MsG0880042734.01.T01 MTR_6g005340 41.989 181 86 5 47 214 1 175 1.81e-29 110
MsG0880042734.01.T01 MTR_3g019610 51.923 104 48 1 51 154 6 107 3.06e-29 107
MsG0880042734.01.T01 MTR_1g085140 37.113 194 118 3 59 249 20 212 3.14e-29 110
MsG0880042734.01.T01 MTR_7g111240 42.767 159 83 5 68 222 25 179 3.19e-29 110
MsG0880042734.01.T01 MTR_2g072560 50.435 115 55 2 48 162 3 115 4.73e-29 107
MsG0880042734.01.T01 MTR_1g079490 39.594 197 108 5 45 232 1 195 7.07e-29 109
MsG0880042734.01.T01 MTR_5g046410 40.223 179 95 4 45 214 1 176 8.80e-29 108
MsG0880042734.01.T01 MTR_5g046430 40.223 179 95 4 45 214 1 176 8.80e-29 108
MsG0880042734.01.T01 MTR_4g017030 40.223 179 95 4 45 214 1 176 8.80e-29 108
MsG0880042734.01.T01 MTR_4g017050 40.223 179 95 4 45 214 1 176 9.48e-29 108
MsG0880042734.01.T01 MTR_4g017040 39.665 179 96 4 45 214 1 176 1.09e-28 108
MsG0880042734.01.T01 MTR_2g031270 44.000 150 77 3 68 214 28 173 1.26e-28 108
MsG0880042734.01.T01 MTR_2g019250 40.223 179 95 4 45 214 1 176 3.95e-28 107
MsG0880042734.01.T01 MTR_1g112200 31.579 209 136 3 48 255 8 210 2.67e-27 105
MsG0880042734.01.T01 MTR_1g023770 37.714 175 99 3 43 214 2 169 3.12e-27 104
MsG0880042734.01.T01 MTR_8g007300 44.030 134 72 2 82 214 47 178 7.30e-27 103
MsG0880042734.01.T01 MTR_1g060120 33.816 207 125 5 55 251 18 222 2.90e-26 102
MsG0880042734.01.T01 MTR_5g036570 31.937 191 125 4 68 254 32 221 8.85e-26 101
MsG0880042734.01.T01 MTR_3g096330 36.111 180 104 3 44 214 552 729 2.75e-25 104
MsG0880042734.01.T01 MTR_6g005350 36.946 203 115 4 47 239 1 200 6.41e-25 99.0
MsG0880042734.01.T01 MTR_2g030895 38.596 171 97 4 86 252 7 173 1.23e-24 97.1
MsG0880042734.01.T01 MTR_2g030865 43.609 133 70 3 86 214 7 138 8.80e-23 92.0
MsG0880042734.01.T01 MTR_2g031300 45.794 107 54 2 86 189 7 112 8.67e-20 83.6
MsG0880042734.01.T01 MTR_2g096110 34.437 151 79 3 91 241 8 138 4.15e-17 76.3
MsG0880042734.01.T01 MTR_2g086640 31.319 182 107 7 40 217 1 168 6.54e-17 77.0
MsG0880042734.01.T01 MTR_4g052770 32.919 161 93 5 78 224 28 187 3.20e-16 75.5
MsG0880042734.01.T01 MTR_4g052780 32.919 161 93 5 78 224 28 187 9.88e-16 73.9
MsG0880042734.01.T01 MTR_2g086620 35.172 145 86 5 78 217 28 169 1.19e-15 73.9
MsG0880042734.01.T01 MTR_2g086630 34.483 145 87 5 78 217 28 169 2.90e-15 72.8
MsG0880042734.01.T01 MTR_7g029500 52.830 53 25 0 197 249 38 90 4.49e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880042734.01.T01 AT5G20630 49.057 212 105 3 45 255 1 210 2.93e-61 192
MsG0880042734.01.T01 AT1G72610 49.474 190 94 2 68 256 20 208 6.32e-56 178
MsG0880042734.01.T01 AT5G39160 39.091 220 119 6 45 253 1 216 3.69e-39 135
MsG0880042734.01.T01 AT5G39130 38.462 221 123 6 45 253 1 220 7.12e-38 132
MsG0880042734.01.T01 AT5G39190 38.462 221 123 6 45 253 1 220 7.76e-38 132
MsG0880042734.01.T01 AT5G39160 38.462 221 123 6 45 253 1 220 1.24e-37 132
MsG0880042734.01.T01 AT5G38960 41.262 206 101 5 64 253 22 223 5.53e-37 130
MsG0880042734.01.T01 AT3G04200 38.356 219 124 5 45 253 6 223 7.14e-37 130
MsG0880042734.01.T01 AT5G26700 42.328 189 102 5 68 253 26 210 1.87e-35 126
MsG0880042734.01.T01 AT3G05930 42.021 188 106 2 68 252 28 215 2.65e-35 126
MsG0880042734.01.T01 AT5G39110 40.331 181 101 3 40 214 1 180 1.10e-34 124
MsG0880042734.01.T01 AT1G02335 39.713 209 114 6 50 249 7 212 1.55e-34 124
MsG0880042734.01.T01 AT3G62020 42.775 173 88 4 53 219 8 175 4.01e-33 120
MsG0880042734.01.T01 AT3G04150 37.559 213 116 5 40 247 5 205 4.82e-33 120
MsG0880042734.01.T01 AT5G39120 39.000 200 103 6 44 230 2 195 5.48e-33 120
MsG0880042734.01.T01 AT3G05950 42.135 178 94 4 45 214 5 181 5.54e-33 120
MsG0880042734.01.T01 AT3G04150 41.566 166 83 3 86 247 63 218 3.55e-32 118
MsG0880042734.01.T01 AT5G39180 37.755 196 107 4 45 230 5 195 4.60e-32 117
MsG0880042734.01.T01 AT5G39150 37.755 196 107 4 45 230 5 195 1.28e-31 116
MsG0880042734.01.T01 AT1G18980 36.239 218 133 4 39 253 5 219 4.85e-31 115
MsG0880042734.01.T01 AT3G04190 44.737 152 78 3 68 214 31 181 5.91e-31 114
MsG0880042734.01.T01 AT5G38940 39.583 192 108 4 68 252 31 221 8.07e-31 114
MsG0880042734.01.T01 AT5G38940 39.583 192 108 4 68 252 55 245 1.51e-30 114
MsG0880042734.01.T01 AT5G39190 35.000 220 110 6 45 253 1 198 1.72e-30 113
MsG0880042734.01.T01 AT4G14630 45.860 157 70 4 68 214 31 182 2.48e-30 113
MsG0880042734.01.T01 AT5G39160 35.000 220 110 6 45 253 1 198 3.10e-30 112
MsG0880042734.01.T01 AT5G61750 37.989 179 95 4 45 214 1 172 1.19e-29 111
MsG0880042734.01.T01 AT3G62020 45.652 138 69 3 83 219 14 146 2.15e-29 109
MsG0880042734.01.T01 AT5G38910 37.981 208 109 6 58 252 19 219 6.66e-29 109
MsG0880042734.01.T01 AT5G38930 38.542 192 110 4 68 252 31 221 1.06e-28 108
MsG0880042734.01.T01 AT1G09560 39.429 175 101 3 60 230 18 191 2.84e-28 107
MsG0880042734.01.T01 AT1G74820 45.522 134 69 3 68 200 41 171 8.32e-28 106
MsG0880042734.01.T01 AT3G04180 37.572 173 101 3 86 252 50 221 1.60e-27 105
MsG0880042734.01.T01 AT1G18970 35.681 213 128 5 47 253 10 219 1.73e-27 105
MsG0880042734.01.T01 AT3G04170 42.581 155 74 4 68 214 31 178 1.65e-26 103
MsG0880042734.01.T01 AT1G10460 36.000 150 94 2 83 230 42 191 1.02e-25 100
MsG0880042734.01.T01 AT3G10080 35.673 171 103 3 85 252 53 219 2.22e-22 92.4
MsG0880042734.01.T01 AT3G10080 35.673 171 103 3 85 252 124 290 1.35e-21 91.7
MsG0880042734.01.T01 AT5G38910 30.698 215 98 6 48 252 9 182 2.95e-14 69.7

Find 44 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCCTCATCTTTGAAAAT+TGG 0.170431 8:-13350309 None:intergenic
ATGAAGCTTAAGGTTAATTT+TGG 0.189584 8:+13350925 MsG0880042734.01.T01:CDS
CACTTAATCCTGGTGATGTT+TGG 0.277337 8:+13350725 MsG0880042734.01.T01:CDS
ACTTAATCCTGGTGATGTTT+GGG 0.337157 8:+13350726 MsG0880042734.01.T01:CDS
GAGTTTGTCGACGTGTTTGC+AGG 0.363951 8:-13350502 None:intergenic
TCCACAAATTATGAAGCTTA+AGG 0.372687 8:+13350915 MsG0880042734.01.T01:CDS
ATCGTTTCCAGACACCCCTT+CGG 0.376629 8:+13350414 MsG0880042734.01.T01:CDS
TCGTTTCCAGACACCCCTTC+GGG 0.386894 8:+13350415 MsG0880042734.01.T01:CDS
ACCTTAAGCTTCATAATTTG+TGG 0.389226 8:-13350916 None:intergenic
GGATAGCCCGAAGGGGTGTC+TGG 0.391633 8:-13350421 None:intergenic
ATCAGAATCAATAGCATTTG+AGG 0.419441 8:-13350371 None:intergenic
AGAATAGATCTTGGCTTCAA+TGG 0.435930 8:+13350598 MsG0880042734.01.T01:CDS
TTTATACTTAACACTGGTCC+TGG 0.439650 8:+13350772 MsG0880042734.01.T01:CDS
GAGGGAGTGGGTACAATGGT+TGG 0.467753 8:+13348477 None:intergenic
GAATAAGCAACAGCATTTCC+AGG 0.471369 8:-13350790 None:intergenic
ATTATAAATTCGCCTACGCC+AGG 0.477090 8:-13350643 None:intergenic
TTTATGAAAACACTTAATCC+TGG 0.483307 8:+13350715 MsG0880042734.01.T01:CDS
TCTGTAGCAAGAATAGATCT+TGG 0.497563 8:+13350589 MsG0880042734.01.T01:CDS
CGATGAGGTGATCGAGTTGA+GGG 0.498041 8:+13348515 MsG0880042734.01.T01:CDS
TCATCTTTGAAAATTGGATG+TGG 0.498190 8:-13350303 None:intergenic
GGGATAAGAGAATAATAGAT+AGG 0.502801 8:-13350282 None:intergenic
ATTCAAGGTCAAATGACTGC+TGG 0.516733 8:+13350670 MsG0880042734.01.T01:CDS
AGGGAGTGGGTACAATGGTT+GGG 0.521572 8:+13348478 None:intergenic
ATAAAATGCAAAAGTCCTGT+TGG 0.527650 8:-13350754 None:intergenic
GATAAGATACCAGGTCTCAA+TGG 0.527717 8:+13350556 MsG0880042734.01.T01:CDS
GGTACAATGGTTGGGCATGT+TGG 0.539856 8:+13348486 None:intergenic
ATACCAGGTCTCAATGGACT+TGG 0.540360 8:+13350562 MsG0880042734.01.T01:CDS
GAATTTATAATCGTCATTCA+AGG 0.543864 8:+13350655 MsG0880042734.01.T01:CDS
GTACCAATGCACACTCATCC+TGG 0.556822 8:+13350625 MsG0880042734.01.T01:CDS
GCGATGAGGTGATCGAGTTG+AGG 0.561821 8:+13348514 MsG0880042734.01.T01:CDS
CATCTTTGAAAATTGGATGT+GGG 0.568652 8:-13350302 None:intergenic
TTAAGCTTCATAATTTGTGG+AGG 0.583672 8:-13350913 None:intergenic
ACTTGCATGGATAGCCCGAA+GGG 0.586847 8:-13350429 None:intergenic
AAAGTGAATCGATCGAGTGA+CGG 0.588816 8:+13348543 MsG0880042734.01.T01:CDS
GACTTGCATGGATAGCCCGA+AGG 0.590826 8:-13350430 None:intergenic
GTAAAAGTCGATAAGATACC+AGG 0.594356 8:+13350547 MsG0880042734.01.T01:CDS
ATGGTTGGGCATGTTGGCAG+TGG 0.597417 8:+13348492 MsG0880042734.01.T01:CDS
AGTGACGGAGATCATGCGAT+TGG 0.603283 8:+13348558 MsG0880042734.01.T01:CDS
ACGCCAGGATGAGTGTGCAT+TGG 0.618287 8:-13350628 None:intergenic
ATGCACACTCATCCTGGCGT+AGG 0.634982 8:+13350631 MsG0880042734.01.T01:CDS
TTCGGGCTATCCATGCAAGT+CGG 0.659661 8:+13350432 MsG0880042734.01.T01:CDS
GCATGTTGGCAGTGGCGATG+AGG 0.666909 8:+13348500 MsG0880042734.01.T01:CDS
ATACCAAGTCCATTGAGACC+TGG 0.667082 8:-13350565 None:intergenic
CTTGCATGGATAGCCCGAAG+GGG 0.715273 8:-13350428 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTATATTTT+AGG + Chr8:13349253-13349272 MsG0880042734.01.T01:intron 0.0%
!! AAAAAAAAACAAGATTAAAT+AGG + Chr8:13349184-13349203 MsG0880042734.01.T01:intron 10.0%
!! ATGTTTCAAATTATAAAATT+TGG + Chr8:13349624-13349643 MsG0880042734.01.T01:intron 10.0%
!!! ATATGAACTAATTTTTTTTT+TGG - Chr8:13349739-13349758 None:intergenic 10.0%
!!! TTTTTTTTTATATTTTAGGA+CGG + Chr8:13349257-13349276 MsG0880042734.01.T01:intron 10.0%
!! ATTCAAATTGTTAATAACAT+TGG + Chr8:13349395-13349414 MsG0880042734.01.T01:intron 15.0%
!! TTATTAACAATTTGAATAGA+GGG - Chr8:13349392-13349411 None:intergenic 15.0%
!! TTTCTTCTTATAATTTGAAA+CGG - Chr8:13348951-13348970 None:intergenic 15.0%
!!! TATAATTTGAAACATGTTTT+TGG - Chr8:13349619-13349638 None:intergenic 15.0%
!!! TTAACTGATTTTTTGTTATT+TGG + Chr8:13350844-13350863 MsG0880042734.01.T01:CDS 15.0%
!!! TTAATTAATGTTACCATTTT+TGG - Chr8:13350134-13350153 None:intergenic 15.0%
!!! TTAGTGCTTTTTTTAATTAA+GGG - Chr8:13349353-13349372 None:intergenic 15.0%
!!! TTTAGTGCTTTTTTTAATTA+AGG - Chr8:13349354-13349373 None:intergenic 15.0%
!! AAAAATGAATCTGAGAAATA+TGG + Chr8:13348705-13348724 MsG0880042734.01.T01:intron 20.0%
!! AAAATGAATCTGAGAAATAT+GGG + Chr8:13348706-13348725 MsG0880042734.01.T01:intron 20.0%
!! AAAATTGAAAATGCATAAGT+GGG + Chr8:13349542-13349561 MsG0880042734.01.T01:intron 20.0%
!! AGAAATAGATACATATAGAT+AGG + Chr8:13350155-13350174 MsG0880042734.01.T01:intron 20.0%
!! AGAAGATTTCAAATTGTATA+TGG + Chr8:13349566-13349585 MsG0880042734.01.T01:intron 20.0%
!! GCAAAGTTATAACTAAAAAA+TGG - Chr8:13349928-13349947 None:intergenic 20.0%
!! GTTATTAACAATTTGAATAG+AGG - Chr8:13349393-13349412 None:intergenic 20.0%
!! TATAAATTAGTTACGTAGAT+AGG + Chr8:13350182-13350201 MsG0880042734.01.T01:intron 20.0%
!!! AACTAATTTTTTTTTTGGTC+AGG - Chr8:13349734-13349753 None:intergenic 20.0%
!!! GTTTTCATAAAAACTTTTGT+AGG - Chr8:13350706-13350725 None:intergenic 20.0%
!!! GTTTTGAATGAATTTCTTTT+TGG + Chr8:13349787-13349806 MsG0880042734.01.T01:intron 20.0%
!!! TATATTCGAACAAGTTTTAA+TGG + Chr8:13349453-13349472 MsG0880042734.01.T01:intron 20.0%
!!! TTGGACAAAACAAATTTTAA+TGG + Chr8:13349414-13349433 MsG0880042734.01.T01:intron 20.0%
! AAATTGAAAATGCATAAGTG+GGG + Chr8:13349543-13349562 MsG0880042734.01.T01:intron 25.0%
! ACACCTTCATAAAAATTAGT+TGG + Chr8:13349954-13349973 MsG0880042734.01.T01:intron 25.0%
! ATAGATACATATAGATAGGT+TGG + Chr8:13350159-13350178 MsG0880042734.01.T01:intron 25.0%
! ATAGTGTTAATATTCTTGCA+AGG - Chr8:13350252-13350271 None:intergenic 25.0%
! CAAAGACTGAACAAATAAAT+TGG + Chr8:13349301-13349320 MsG0880042734.01.T01:intron 25.0%
! CATATGAATCATGCAAAATT+AGG + Chr8:13348736-13348755 MsG0880042734.01.T01:intron 25.0%
! GAAAATTGAAAATGCATAAG+TGG + Chr8:13349541-13349560 MsG0880042734.01.T01:intron 25.0%
! GAATTTATAATCGTCATTCA+AGG + Chr8:13350655-13350674 MsG0880042734.01.T01:CDS 25.0%
! GATGTAAAAACAATTTGCAT+TGG + Chr8:13349060-13349079 MsG0880042734.01.T01:intron 25.0%
! TAAATAGGATAAACGTTTGT+CGG + Chr8:13349199-13349218 MsG0880042734.01.T01:intron 25.0%
! TAATGGAAAATGGCAAAATT+TGG + Chr8:13349470-13349489 MsG0880042734.01.T01:intron 25.0%
! TTTATGAAAACACTTAATCC+TGG + Chr8:13350715-13350734 MsG0880042734.01.T01:CDS 25.0%
!! ACACCAACTAATTTTTATGA+AGG - Chr8:13349960-13349979 None:intergenic 25.0%
!! ATGAAGCTTAAGGTTAATTT+TGG + Chr8:13350925-13350944 MsG0880042734.01.T01:CDS 25.0%
!! TCAAAACAATTGAGTCATTT+TGG - Chr8:13349774-13349793 None:intergenic 25.0%
!! TTGCATTTTATACTTAACAC+TGG + Chr8:13350766-13350785 MsG0880042734.01.T01:CDS 25.0%
!!! AAGGATGTCATTTTAAAACA+AGG + Chr8:13350081-13350100 MsG0880042734.01.T01:intron 25.0%
!!! AGGATGTCATTTTAAAACAA+GGG + Chr8:13350082-13350101 MsG0880042734.01.T01:intron 25.0%
!!! GAACAAGTTTTAATGGAAAA+TGG + Chr8:13349460-13349479 MsG0880042734.01.T01:intron 25.0%
!!! TAATGTTACCATTTTTGGTA+TGG - Chr8:13350129-13350148 None:intergenic 25.0%
!!! TGGATTTTGAAAATTTGAAC+AGG - Chr8:13349330-13349349 None:intergenic 25.0%
!!! TTTTTATATTTTAGGACGGA+AGG + Chr8:13349261-13349280 MsG0880042734.01.T01:intron 25.0%
AAAACCCTAGATATATAGTG+GGG + Chr8:13350042-13350061 MsG0880042734.01.T01:intron 30.0%
AAAATGGAAATCACAACTTG+AGG - Chr8:13349912-13349931 None:intergenic 30.0%
ACCTTAAGCTTCATAATTTG+TGG - Chr8:13350919-13350938 None:intergenic 30.0%
ATAAAATGCAAAAGTCCTGT+TGG - Chr8:13350757-13350776 None:intergenic 30.0%
ATATTGAGCCATACCAAAAA+TGG + Chr8:13350118-13350137 MsG0880042734.01.T01:intron 30.0%
ATCAGAATCAATAGCATTTG+AGG - Chr8:13350374-13350393 None:intergenic 30.0%
CAAAACCCTAGATATATAGT+GGG + Chr8:13350041-13350060 MsG0880042734.01.T01:intron 30.0%
GGGATAAGAGAATAATAGAT+AGG - Chr8:13350285-13350304 None:intergenic 30.0%
TCCACAAATTATGAAGCTTA+AGG + Chr8:13350915-13350934 MsG0880042734.01.T01:CDS 30.0%
TTGCTAAAAGACACTTTCAT+GGG - Chr8:13349883-13349902 None:intergenic 30.0%
! AAGCTTAAGGTTAATTTTGG+TGG + Chr8:13350928-13350947 MsG0880042734.01.T01:CDS 30.0%
! ACATCCAATTTTCAAAGATG+AGG + Chr8:13350305-13350324 MsG0880042734.01.T01:CDS 30.0%
! CATCTTTGAAAATTGGATGT+GGG - Chr8:13350305-13350324 None:intergenic 30.0%
! TCATCCTCATCTTTGAAAAT+TGG - Chr8:13350312-13350331 None:intergenic 30.0%
! TCATCTTTGAAAATTGGATG+TGG - Chr8:13350306-13350325 None:intergenic 30.0%
! TTAAGCTTCATAATTTGTGG+AGG - Chr8:13350916-13350935 None:intergenic 30.0%
! TTAGAAGATAGAAAGCCTTT+TGG + Chr8:13348760-13348779 MsG0880042734.01.T01:intron 30.0%
!! AAACAATTTGCATTGGCTAT+TGG + Chr8:13349067-13349086 MsG0880042734.01.T01:intron 30.0%
!! GTCGGTTTTGCTTATATTTT+GGG + Chr8:13349217-13349236 MsG0880042734.01.T01:intron 30.0%
!!! AATTTGGTGGATTTTGGATT+TGG + Chr8:13349486-13349505 MsG0880042734.01.T01:intron 30.0%
!!! GTGCTTTTTTTAATTAAGGG+TGG - Chr8:13349350-13349369 None:intergenic 30.0%
!!! TATCTTATCGACTTTTACAG+TGG - Chr8:13350545-13350564 None:intergenic 30.0%
!!! TGTCGGTTTTGCTTATATTT+TGG + Chr8:13349216-13349235 MsG0880042734.01.T01:intron 30.0%
AAACCCTAGATATATAGTGG+GGG + Chr8:13350043-13350062 MsG0880042734.01.T01:intron 35.0%
AAAGGGAAAACAAATTCAGC+AGG + Chr8:13348785-13348804 MsG0880042734.01.T01:intron 35.0%
AGAATAGATCTTGGCTTCAA+TGG + Chr8:13350598-13350617 MsG0880042734.01.T01:CDS 35.0%
ATGGACAAAAGGCAAAGTTA+GGG + Chr8:13348632-13348651 MsG0880042734.01.T01:intron 35.0%
ATGTCATGAACACAATACGA+GGG + Chr8:13348904-13348923 MsG0880042734.01.T01:intron 35.0%
CTATCACAATCCCATAAACA+TGG - Chr8:13348612-13348631 None:intergenic 35.0%
CTTGCTAAAAGACACTTTCA+TGG - Chr8:13349884-13349903 None:intergenic 35.0%
GATTGTGATAGATGGACAAA+AGG + Chr8:13348621-13348640 MsG0880042734.01.T01:intron 35.0%
GCAAAACCCTAGATATATAG+TGG + Chr8:13350040-13350059 MsG0880042734.01.T01:intron 35.0%
GTAAAAGTCGATAAGATACC+AGG + Chr8:13350547-13350566 MsG0880042734.01.T01:CDS 35.0%
GTTTATGGGATTGTGATAGA+TGG + Chr8:13348613-13348632 MsG0880042734.01.T01:intron 35.0%
TAGGGATTAGATGAGGAAAT+GGG + Chr8:13348650-13348669 MsG0880042734.01.T01:intron 35.0%
TCGCAATTAAGCAAGCTATA+AGG + Chr8:13350218-13350237 MsG0880042734.01.T01:intron 35.0%
TCTGTAGCAAGAATAGATCT+TGG + Chr8:13350589-13350608 MsG0880042734.01.T01:CDS 35.0%
TGAAATGAAATGCAACGTGA+GGG + Chr8:13348818-13348837 MsG0880042734.01.T01:intron 35.0%
TGCCACATCAAAAAATCCAA+CGG - Chr8:13348871-13348890 None:intergenic 35.0%
TGGAAAATGGCAAAATTTGG+TGG + Chr8:13349473-13349492 MsG0880042734.01.T01:intron 35.0%
TTAGGGATTAGATGAGGAAA+TGG + Chr8:13348649-13348668 MsG0880042734.01.T01:intron 35.0%
TTTATACTTAACACTGGTCC+TGG + Chr8:13350772-13350791 MsG0880042734.01.T01:CDS 35.0%
! AAGAGTAGTCTTTTGAACTG+TGG - Chr8:13350887-13350906 None:intergenic 35.0%
! ACTTAATCCTGGTGATGTTT+GGG + Chr8:13350726-13350745 MsG0880042734.01.T01:CDS 35.0%
! GCTAATGATTTTGTGTTCTC+TGG + Chr8:13350469-13350488 MsG0880042734.01.T01:CDS 35.0%
! TTGTTTTCCCTTTCTCCAAA+AGG - Chr8:13348778-13348797 None:intergenic 35.0%
!! ATAGAAAGCCTTTTGGAGAA+AGG + Chr8:13348767-13348786 MsG0880042734.01.T01:intron 35.0%
!! TAGAAAGCCTTTTGGAGAAA+GGG + Chr8:13348768-13348787 MsG0880042734.01.T01:intron 35.0%
!! TGGTGTTCGAATTCTAGAAT+CGG - Chr8:13349599-13349618 None:intergenic 35.0%
!!! ACAATTGAGTCATTTTGGCT+TGG - Chr8:13349769-13349788 None:intergenic 35.0%
!!! TGGCAAAATTTGGTGGATTT+TGG + Chr8:13349480-13349499 MsG0880042734.01.T01:intron 35.0%
AAAGTGAATCGATCGAGTGA+CGG + Chr8:13348543-13348562 MsG0880042734.01.T01:CDS 40.0%
AAGTGAGTCCAAGAGACATT+AGG + Chr8:13349660-13349679 MsG0880042734.01.T01:intron 40.0%
AGGGATTAGATGAGGAAATG+GGG + Chr8:13348651-13348670 MsG0880042734.01.T01:intron 40.0%
AGTGAGTCCAAGAGACATTA+GGG + Chr8:13349661-13349680 MsG0880042734.01.T01:intron 40.0%
ATTAGATGAGGAAATGGGGT+TGG + Chr8:13348655-13348674 MsG0880042734.01.T01:intron 40.0%
ATTATAAATTCGCCTACGCC+AGG - Chr8:13350646-13350665 None:intergenic 40.0%
ATTCAAGGTCAAATGACTGC+TGG + Chr8:13350670-13350689 MsG0880042734.01.T01:CDS 40.0%
CATGTCATGAACACAATACG+AGG + Chr8:13348903-13348922 MsG0880042734.01.T01:intron 40.0%
CGAATGAACGCCATGTTTAT+GGG + Chr8:13348599-13348618 MsG0880042734.01.T01:intron 40.0%
CTAAAAGACACTTTCATGGG+TGG - Chr8:13349880-13349899 None:intergenic 40.0%
GAAATGAAATGCAACGTGAG+GGG + Chr8:13348819-13348838 MsG0880042734.01.T01:intron 40.0%
GAATAAGCAACAGCATTTCC+AGG - Chr8:13350793-13350812 None:intergenic 40.0%
GATAAGATACCAGGTCTCAA+TGG + Chr8:13350556-13350575 MsG0880042734.01.T01:CDS 40.0%
GATGGACAAAAGGCAAAGTT+AGG + Chr8:13348631-13348650 MsG0880042734.01.T01:intron 40.0%
GCAAAGTTAGGGATTAGATG+AGG + Chr8:13348643-13348662 MsG0880042734.01.T01:intron 40.0%
GCCACATCAAAAAATCCAAC+GGG - Chr8:13348870-13348889 None:intergenic 40.0%
GTGAAATGAAATGCAACGTG+AGG + Chr8:13348817-13348836 MsG0880042734.01.T01:intron 40.0%
TCGAATGAACGCCATGTTTA+TGG + Chr8:13348598-13348617 MsG0880042734.01.T01:intron 40.0%
TCTCCCCCACTATATATCTA+GGG - Chr8:13350049-13350068 None:intergenic 40.0%
TTCTCCCCCACTATATATCT+AGG - Chr8:13350050-13350069 None:intergenic 40.0%
! CACTTAATCCTGGTGATGTT+TGG + Chr8:13350725-13350744 MsG0880042734.01.T01:CDS 40.0%
! GGCTATTGGTTATGAAGAAG+TGG + Chr8:13349081-13349100 MsG0880042734.01.T01:intron 40.0%
! GTCTTTTGAACTGTGGAAGT+TGG - Chr8:13350880-13350899 None:intergenic 40.0%
! TTTTCACCCCTAATGTCTCT+TGG - Chr8:13349671-13349690 None:intergenic 40.0%
! TTTTTGATGTGGCAAAACCG+AGG + Chr8:13348877-13348896 MsG0880042734.01.T01:intron 40.0%
!! AAGGTTAATTTTGGTGGCAG+TGG + Chr8:13350934-13350953 MsG0880042734.01.T01:CDS 40.0%
!! ACATTGTTTTCCGACTTGCA+TGG - Chr8:13350445-13350464 None:intergenic 40.0%
!! ATCATGCGATTGGTAATGTG+TGG + Chr8:13348568-13348587 MsG0880042734.01.T01:intron 40.0%
!! GATGTTTGGGTTTTTCCAAC+AGG + Chr8:13350739-13350758 MsG0880042734.01.T01:CDS 40.0%
!! GGCTCAATATTTGACTTGCT+AGG - Chr8:13350108-13350127 None:intergenic 40.0%
!! GGTGTTCGAATTCTAGAATC+GGG - Chr8:13349598-13349617 None:intergenic 40.0%
!! TCATGCGATTGGTAATGTGT+GGG + Chr8:13348569-13348588 MsG0880042734.01.T01:intron 40.0%
!!! ACCCGTTGGATTTTTTGATG+TGG + Chr8:13348866-13348885 MsG0880042734.01.T01:intron 40.0%
!!! TGGTGGATTTTGGATTTGGA+AGG + Chr8:13349490-13349509 MsG0880042734.01.T01:intron 40.0%
AGGAAATGGGGTTGGAAAAG+AGG + Chr8:13348663-13348682 MsG0880042734.01.T01:intron 45.0%
ATACCAAGTCCATTGAGACC+TGG - Chr8:13350568-13350587 None:intergenic 45.0%
GCATTTCATTTCACGTAGCG+AGG - Chr8:13348811-13348830 None:intergenic 45.0%
GGAAAAACCCAAACATCACC+AGG - Chr8:13350736-13350755 None:intergenic 45.0%
GGTTGGAAAAGAGGTAGCTA+GGG + Chr8:13348672-13348691 MsG0880042734.01.T01:intron 45.0%
GTGAGTCCAAGAGACATTAG+GGG + Chr8:13349662-13349681 MsG0880042734.01.T01:intron 45.0%
GTGTTCATGACATGTCACCT+CGG - Chr8:13348897-13348916 None:intergenic 45.0%
TTTCCTGCACGTGAGCAATT+GGG - Chr8:13349815-13349834 None:intergenic 45.0%
! ATACCAGGTCTCAATGGACT+TGG + Chr8:13350562-13350581 MsG0880042734.01.T01:CDS 45.0%
! ATATAGTGGGGGAGAAGTGT+AGG + Chr8:13350054-13350073 MsG0880042734.01.T01:intron 45.0%
! TATAGTGGGGGAGAAGTGTA+GGG + Chr8:13350055-13350074 MsG0880042734.01.T01:intron 45.0%
! TTTTCCTGCACGTGAGCAAT+TGG - Chr8:13349816-13349835 None:intergenic 45.0%
!! CATGCGATTGGTAATGTGTG+GGG + Chr8:13348570-13348589 MsG0880042734.01.T01:intron 45.0%
!! GATTGGTAATGTGTGGGGTT+TGG + Chr8:13348575-13348594 MsG0880042734.01.T01:intron 45.0%
!! AGTATATATATATATATATA+TGG + Chr8:13349841-13349860 MsG0880042734.01.T01:intron 5.0%
!! GTATATATATATATATATAT+GGG + Chr8:13349842-13349861 MsG0880042734.01.T01:intron 5.0%
ACTTGCATGGATAGCCCGAA+GGG - Chr8:13350432-13350451 None:intergenic 50.0%
ATCGTTTCCAGACACCCCTT+CGG + Chr8:13350414-13350433 MsG0880042734.01.T01:CDS 50.0%
ATGAAATGCAACGTGAGGGG+AGG + Chr8:13348822-13348841 MsG0880042734.01.T01:intron 50.0%
GAGTTTGTCGACGTGTTTGC+AGG - Chr8:13350505-13350524 None:intergenic 50.0%
GGGTTGGAAAAGAGGTAGCT+AGG + Chr8:13348671-13348690 MsG0880042734.01.T01:intron 50.0%
GTACCAATGCACACTCATCC+TGG + Chr8:13350625-13350644 MsG0880042734.01.T01:CDS 50.0%
TGAAATGCAACGTGAGGGGA+GGG + Chr8:13348823-13348842 MsG0880042734.01.T01:intron 50.0%
TTCCTGCACGTGAGCAATTG+GGG - Chr8:13349814-13349833 None:intergenic 50.0%
TTCGGGCTATCCATGCAAGT+CGG + Chr8:13350432-13350451 MsG0880042734.01.T01:CDS 50.0%
! AGTGACGGAGATCATGCGAT+TGG + Chr8:13348558-13348577 MsG0880042734.01.T01:CDS 50.0%
! ATAGTGGGGGAGAAGTGTAG+GGG + Chr8:13350056-13350075 MsG0880042734.01.T01:intron 50.0%
! CAATAGGAAGGTGTACCCGT+TGG + Chr8:13348852-13348871 MsG0880042734.01.T01:intron 50.0%
!! CGATGAGGTGATCGAGTTGA+GGG + Chr8:13348515-13348534 MsG0880042734.01.T01:CDS 50.0%
ACGCCAGGATGAGTGTGCAT+TGG - Chr8:13350631-13350650 None:intergenic 55.0%
ATGCACACTCATCCTGGCGT+AGG + Chr8:13350631-13350650 MsG0880042734.01.T01:CDS 55.0%
ATGGTTGGGCATGTTGGCAG+TGG + Chr8:13348492-13348511 MsG0880042734.01.T01:CDS 55.0%
CTTGCATGGATAGCCCGAAG+GGG - Chr8:13350431-13350450 None:intergenic 55.0%
GAAATGCAACGTGAGGGGAG+GGG + Chr8:13348824-13348843 MsG0880042734.01.T01:intron 55.0%
GACTTGCATGGATAGCCCGA+AGG - Chr8:13350433-13350452 None:intergenic 55.0%
GGAGGGGAGGAACAATAGGA+AGG + Chr8:13348840-13348859 MsG0880042734.01.T01:intron 55.0%
TCCTGCACGTGAGCAATTGG+GGG - Chr8:13349813-13349832 None:intergenic 55.0%
TCGTTTCCAGACACCCCTTC+GGG + Chr8:13350415-13350434 MsG0880042734.01.T01:CDS 55.0%
! GCGATGAGGTGATCGAGTTG+AGG + Chr8:13348514-13348533 MsG0880042734.01.T01:CDS 55.0%
! GGGGAGAAGTGTAGGGGATA+AGG + Chr8:13350062-13350081 MsG0880042734.01.T01:intron 55.0%
GAGGGGAGGGGAGGAACAAT+AGG + Chr8:13348836-13348855 MsG0880042734.01.T01:intron 60.0%
GCATGTTGGCAGTGGCGATG+AGG + Chr8:13348500-13348519 MsG0880042734.01.T01:CDS 60.0%
ATGCAACGTGAGGGGAGGGG+AGG + Chr8:13348827-13348846 MsG0880042734.01.T01:intron 65.0%
GCCCCCAATTGCTCACGTGC+AGG + Chr8:13349809-13349828 MsG0880042734.01.T01:intron 65.0%
!! GGATAGCCCGAAGGGGTGTC+TGG - Chr8:13350424-13350443 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 13348492 13350960 13348492 ID=MsG0880042734.01;Name=MsG0880042734.01
Chr8 mRNA 13348492 13350960 13348492 ID=MsG0880042734.01.T01;Parent=MsG0880042734.01;Name=MsG0880042734.01.T01;_AED=0.39;_eAED=0.39;_QI=0|0|0|0.5|0|0.5|2|0|256
Chr8 exon 13348492 13348579 13348492 ID=MsG0880042734.01.T01:exon:823;Parent=MsG0880042734.01.T01
Chr8 exon 13350278 13350960 13350278 ID=MsG0880042734.01.T01:exon:824;Parent=MsG0880042734.01.T01
Chr8 CDS 13348492 13348579 13348492 ID=MsG0880042734.01.T01:cds;Parent=MsG0880042734.01.T01
Chr8 CDS 13350278 13350960 13350278 ID=MsG0880042734.01.T01:cds;Parent=MsG0880042734.01.T01
Gene Sequence

>MsG0880042734.01.T01

ATGGTTGGGCATGTTGGCAGTGGCGATGAGGTGATCGAGTTGAGGGACTGCAAAGTGAATCGATCGAGTGACGGAGATCATGCGATTGCAAACCTATCTATTATTCTCTTATCCCACATCCAATTTTCAAAGATGAGGATGATTCAAACTATTCTTTTCTTTTTTGCTCTTATCTCATATACCTCAAATGCTATTGATTCTGATGATTTCTGTGTAGCAGACTTATCGTTTCCAGACACCCCTTCGGGCTATCCATGCAAGTCGGAAAACAATGTAACAGCTAATGATTTTGTGTTCTCTGGCTTAAATATACCTGCAAACACGTCGACAAACTCTTTTAAAGTTGCATTTACCACTGTAAAAGTCGATAAGATACCAGGTCTCAATGGACTTGGTATCTCTGTAGCAAGAATAGATCTTGGCTTCAATGGAAGTGTACCAATGCACACTCATCCTGGCGTAGGCGAATTTATAATCGTCATTCAAGGTCAAATGACTGCTGGATTTATCACACCTACAAAAGTTTTTATGAAAACACTTAATCCTGGTGATGTTTGGGTTTTTCCAACAGGACTTTTGCATTTTATACTTAACACTGGTCCTGGAAATGCTGTTGCTTATTCTGCATATGCTAGCTCAAATTTTAACACTCATTTAACTGATTTTTTGTTATTTGGTAACAACTTACCAACTTCCACAGTTCAAAAGACTACTCTTCTTGATCCTCCACAAATTATGAAGCTTAAGGTTAATTTTGGTGGCAGTGGCTAG
Protein sequence

>MsG0880042734.01.T01

MVGHVGSGDEVIELRDCKVNRSSDGDHAIANLSIILLSHIQFSKMRMIQTILFFFALISYTSNAIDSDDFCVADLSFPDTPSGYPCKSENNVTANDFVFSGLNIPANTSTNSFKVAFTTVKVDKIPGLNGLGISVARIDLGFNGSVPMHTHPGVGEFIIVIQGQMTAGFITPTKVFMKTLNPGDVWVFPTGLLHFILNTGPGNAVAYSAYASSNFNTHLTDFLLFGNNLPTSTVQKTTLLDPPQIMKLKVNFGGSG*