Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034465.01.T01 | XP_024641767.1 | 83.871 | 496 | 79 | 1 | 1 | 496 | 57 | 551 | 0 | 865 |
| MsG0680034465.01.T01 | XP_024641767.1 | 30 | 190 | 132 | 1 | 305 | 493 | 44 | 233 | 6.18E-18 | 99.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034465.01.T01 | A0A396HN06 | 83.871 | 496 | 79 | 1 | 1 | 496 | 57 | 551 | 0.0 | 865 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480022802.01 | MsG0680034465.01 | 0.813864 | 2.062236e-51 | 1.362377e-48 |
| MsG0680034463.01 | MsG0680034465.01 | 0.837246 | 5.819473e-57 | 7.514222e-54 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034465.01.T01 | MTR_6g069300 | 83.603 | 494 | 80 | 1 | 1 | 494 | 57 | 549 | 0.0 | 858 |
| MsG0680034465.01.T01 | MTR_6g069300 | 31.880 | 367 | 212 | 4 | 165 | 494 | 44 | 409 | 3.65e-50 | 181 |
| MsG0680034465.01.T01 | MTR_6g068820 | 83.468 | 496 | 38 | 3 | 1 | 496 | 1 | 452 | 0.0 | 829 |
| MsG0680034465.01.T01 | MTR_0110s0060 | 74.194 | 496 | 127 | 1 | 1 | 496 | 48 | 542 | 0.0 | 780 |
| MsG0680034465.01.T01 | MTR_0110s0060 | 29.474 | 190 | 133 | 1 | 305 | 493 | 35 | 224 | 5.96e-21 | 96.3 |
| MsG0680034465.01.T01 | MTR_6g079440 | 73.387 | 496 | 131 | 1 | 1 | 496 | 47 | 541 | 0.0 | 766 |
| MsG0680034465.01.T01 | MTR_6g079440 | 30.851 | 188 | 129 | 1 | 305 | 491 | 34 | 221 | 3.19e-23 | 103 |
| MsG0680034465.01.T01 | MTR_6g061120 | 83.252 | 412 | 68 | 1 | 85 | 496 | 1 | 411 | 0.0 | 699 |
| MsG0680034465.01.T01 | MTR_6g061120 | 32.391 | 389 | 258 | 2 | 37 | 423 | 23 | 408 | 3.84e-65 | 216 |
| MsG0680034465.01.T01 | MTR_6g061120 | 33.815 | 346 | 226 | 2 | 6 | 350 | 62 | 405 | 3.02e-53 | 185 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034465.01.T01 | AT1G62670 | 36.484 | 529 | 300 | 2 | 2 | 495 | 75 | 602 | 1.74e-124 | 377 |
| MsG0680034465.01.T01 | AT1G62670 | 26.703 | 367 | 234 | 1 | 165 | 496 | 62 | 428 | 5.19e-45 | 167 |
| MsG0680034465.01.T01 | AT1G62670 | 29.630 | 297 | 209 | 0 | 199 | 495 | 61 | 357 | 4.90e-38 | 147 |
| MsG0680034465.01.T01 | AT1G62670 | 36.484 | 529 | 300 | 2 | 2 | 495 | 75 | 602 | 1.74e-124 | 377 |
| MsG0680034465.01.T01 | AT1G62670 | 26.703 | 367 | 234 | 1 | 165 | 496 | 62 | 428 | 5.19e-45 | 167 |
| MsG0680034465.01.T01 | AT1G62670 | 29.630 | 297 | 209 | 0 | 199 | 495 | 61 | 357 | 4.90e-38 | 147 |
| MsG0680034465.01.T01 | AT1G62930 | 36.415 | 530 | 301 | 2 | 2 | 496 | 74 | 602 | 2.58e-123 | 374 |
| MsG0680034465.01.T01 | AT1G62930 | 30.270 | 370 | 223 | 1 | 162 | 496 | 58 | 427 | 2.60e-58 | 204 |
| MsG0680034465.01.T01 | AT1G12700 | 36.640 | 494 | 312 | 1 | 2 | 495 | 82 | 574 | 2.85e-122 | 370 |
| MsG0680034465.01.T01 | AT1G12700 | 31.054 | 351 | 240 | 2 | 147 | 496 | 86 | 435 | 2.77e-52 | 187 |
Find 98 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGCAAGGAATTCAGC+CGG | 0.576173 | 6:+88015324 | MsG0680034465.01.T01:CDS |
| AAACGACATGAATGATAAGG+CGG | 0.701233 | 6:+88015597 | MsG0680034465.01.T01:CDS |
| AAAGATTGATGATGGGCTGT+TGG | 0.547381 | 6:+88014580 | MsG0680034465.01.T01:CDS |
| AAAGGGATTTCAACTTAATC+AGG | 0.303746 | 6:+88014487 | MsG0680034465.01.T01:CDS |
| AACATCATCATGAAAGTAAA+GGG | 0.421905 | 6:-88014459 | None:intergenic |
| AACTTAATCAGGTTAGTTAT+GGG | 0.362992 | 6:+88014498 | MsG0680034465.01.T01:CDS |
| AAGACTTAAGGACGCGCAAG+AGG | 0.624830 | 6:+88015393 | MsG0680034465.01.T01:CDS |
| AAGCATAATTGAGTTGACCG+AGG | 0.725827 | 6:-88014334 | None:intergenic |
| AAGGCTATCATTTAGACGTA+TGG | 0.477289 | 6:+88015437 | MsG0680034465.01.T01:CDS |
| AAGTGATCACATCAGCAGGT+TGG | 0.669409 | 6:-88015231 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGGGATTTCAACTTAATC+AGG | + | Chr6:88014487-88014506 | MsG0680034465.01.T01:CDS | 30.0% | |
| ACCAAATTCAAATATTGGTG+GGG | - | Chr6:88014186-88014205 | None:intergenic | 30.0% | |
| ACTATGTAAGACTAAAATGG+TGG | + | Chr6:88015063-88015082 | MsG0680034465.01.T01:CDS | 30.0% | |
| AGAAATGCATTCCAAAAGTA+TGG | + | Chr6:88015108-88015127 | MsG0680034465.01.T01:CDS | 30.0% | |
| ATACTATAGCTATGAACATC+AGG | - | Chr6:88015031-88015050 | None:intergenic | 30.0% | |
| ATCAAAGGTCTATGTCTTAA+TGG | + | Chr6:88014422-88014441 | MsG0680034465.01.T01:CDS | 30.0% | |
| ATGCAAAAATGGAAGACTTA+AGG | + | Chr6:88015381-88015400 | MsG0680034465.01.T01:CDS | 30.0% | |
| ATTGTCCTTACTGTCAAAAA+TGG | + | Chr6:88015513-88015532 | MsG0680034465.01.T01:CDS | 30.0% | |
| ATTTCGAAAGTTACAGGATT+AGG | - | Chr6:88015556-88015575 | None:intergenic | 30.0% | |
| CTTGGTTAAACAAGAGAATA+AGG | + | Chr6:88014964-88014983 | MsG0680034465.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 88014170 | 88015660 | 88014170 | ID=MsG0680034465.01;Name=MsG0680034465.01 |
| Chr6 | mRNA | 88014170 | 88015660 | 88014170 | ID=MsG0680034465.01.T01;Parent=MsG0680034465.01;Name=MsG0680034465.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|496 |
| Chr6 | exon | 88014170 | 88015660 | 88014170 | ID=MsG0680034465.01.T01:exon:7514;Parent=MsG0680034465.01.T01 |
| Chr6 | CDS | 88014170 | 88015660 | 88014170 | ID=MsG0680034465.01.T01:cds;Parent=MsG0680034465.01.T01 |
| Gene Sequence |
>MsG0680034465.01.T01 ATGAACAACCCTACCCCACCAATATTTGAATTTGGTAAGATCTTTGGTTCCCTTGTTAAAATCAACCATTTCAACACTGTTGTTTCCCTTTCTCGACAAATCGAACTCCGAGGTATTCAAACTGATCTTGTTAATTTGAACATATTGGTTAATTGTTTCTGCCACCTCGGTCAACTCAATTATGCTTTTTCTGTATTGAGTAAGATTCTCAAGCTTGGTTTTCACCCTGATACTGTAACAATCAATACACTTATCAAAGGTCTATGTCTTAATGGTAAAGTTAAGGAAGCCCTTTACTTTCATGATGATGTTATAGCAAAGGGATTTCAACTTAATCAGGTTAGTTATGGGACCTTGATTGATGGGTTATGTAAAGTAGGTGAAACAACAGCTGCCATGCAGGTTCTTACAAAGATTGATGATGGGCTGTTGGTTCAGCCTAATGTCATCATGTGGAACACAGTCATTGACAGACTCTGCAAAGATAAGCTTGTAAGTGATGCCTATGATTTATATTCTCAAATGCTTCTCAAGAGAATTTCTCCTGATGTCATCACTTACAATACTTTAATGTATGGCTGTTTTATTGTGGGTCGGTTGAAAGAAGCAGTTGGTTTGTTCAATCAAATGTTGTTGAAAAACATCAAACCAGACGTTTATACATTTAATATACTGGTTGATGGTTTATGTAAGGAAGGAGAGGTTAAAAAAGCTAGAAACGTGTTAGCTATTATGATAAAACAAGGTGTGGATCCTGATGTTGTTACTTATAATTCTTTAATGGATGGGTATTTCTTGGTTAAACAAGAGAATAAGGCCAGATTTGTGTTCAACACTATGGCTAGAAGGGGAGTGACTCCTGATGTTCATAGCTATAGTATCATGATTAATGGACTATGTAAGACTAAAATGGTGGATGAAGCCGTTAATCTCTTCAAAGAAATGCATTCCAAAAGTATGGCTCCTAATACTGTAACATACACTTCACTTATCGATGGTCTTCTCAAATATGGGAGAATCTCTGATGCTTGGGATCTTGTAAATGAGATGCATAATAGAGGCCAACCTGCTGATGTGATCACTTACAATTCCTTATTGGATGCTTTGTGCAAAAACCATCAGGTTGAAAAGGCAATCACATTACTTACGAAGATAAAACAGCAAGGAATTCAGCCGGATATATATACATACACAATACTTGTTGATGGTTTATGCAAAAATGGAAGACTTAAGGACGCGCAAGAGGTTTATCAGATTCTTTTGATTAAAGGCTATCATTTAGACGTATGGATGTATACTGTTATGATTAATGGCCTTTGTAAAGAGAGCTTGTTTGATGAAGCATTGTCCTTACTGTCAAAAATGGAAGACAATGGTTGCACCCCTAATCCTGTAACTTTCGAAATTCTTATCCGTGCTTTGTTTGAAAACGACATGAATGATAAGGCGGTGGAACTTCTTCGCGAAATGATTGCTAGAGGTCTACTATAA |
| Protein sequence |
>MsG0680034465.01.T01 MNNPTPPIFEFGKIFGSLVKINHFNTVVSLSRQIELRGIQTDLVNLNILVNCFCHLGQLNYAFSVLSKILKLGFHPDTVTINTLIKGLCLNGKVKEALYFHDDVIAKGFQLNQVSYGTLIDGLCKVGETTAAMQVLTKIDDGLLVQPNVIMWNTVIDRLCKDKLVSDAYDLYSQMLLKRISPDVITYNTLMYGCFIVGRLKEAVGLFNQMLLKNIKPDVYTFNILVDGLCKEGEVKKARNVLAIMIKQGVDPDVVTYNSLMDGYFLVKQENKARFVFNTMARRGVTPDVHSYSIMINGLCKTKMVDEAVNLFKEMHSKSMAPNTVTYTSLIDGLLKYGRISDAWDLVNEMHNRGQPADVITYNSLLDALCKNHQVEKAITLLTKIKQQGIQPDIYTYTILVDGLCKNGRLKDAQEVYQILLIKGYHLDVWMYTVMINGLCKESLFDEALSLLSKMEDNGCTPNPVTFEILIRALFENDMNDKAVELLREMIARGLL* |
