Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034674.01.T01 | KEH21091.1 | 77.143 | 70 | 15 | 1 | 27 | 95 | 2 | 71 | 3.84E-31 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034674.01.T01 | P42749 | 64.865 | 74 | 25 | 1 | 23 | 95 | 7 | 80 | 2.17E-28 | 103 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034674.01.T01 | A0A072TV79 | 77.143 | 70 | 15 | 1 | 27 | 95 | 2 | 71 | 1.84e-31 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010807.01 | MsG0680034674.01 | 0.820347 | 7.198241e-53 | 5.681224e-50 |
| MsG0680032157.01 | MsG0680034674.01 | 0.803850 | 2.863015e-49 | 1.454103e-46 |
| MsG0680034674.01 | MsG0680034918.01 | 0.816314 | 5.893427e-52 | 4.162119e-49 |
| MsG0680034674.01 | MsG0780038439.01 | 0.809188 | 2.141092e-50 | 1.248781e-47 |
| MsG0680034674.01 | MsG0880043521.01 | 0.800576 | 1.351097e-48 | 6.313109e-46 |
| MsG0480019297.01 | MsG0680034674.01 | 0.805364 | 1.383589e-49 | 7.306038e-47 |
| MsG0480021918.01 | MsG0680034674.01 | 0.820171 | 7.898321e-53 | 6.203794e-50 |
| MsG0480022068.01 | MsG0680034674.01 | 0.802607 | 5.177590e-49 | 2.547156e-46 |
| MsG0480022262.01 | MsG0680034674.01 | 0.800835 | 1.196200e-48 | 5.626265e-46 |
| MsG0480023202.01 | MsG0680034674.01 | 0.804767 | 1.844103e-49 | 9.589786e-47 |
| MsG0480023488.01 | MsG0680034674.01 | 0.810641 | 1.041815e-50 | 6.314125e-48 |
| MsG0580024038.01 | MsG0680034674.01 | 0.820937 | 5.267764e-53 | 4.226518e-50 |
| MsG0380015332.01 | MsG0680034674.01 | 0.805724 | 1.162580e-49 | 6.196518e-47 |
| MsG0380016517.01 | MsG0680034674.01 | 0.822262 | 2.602357e-53 | 2.167292e-50 |
| MsG0580027745.01 | MsG0680034674.01 | 0.801043 | 1.084397e-48 | 5.127724e-46 |
| MsG0280007072.01 | MsG0680034674.01 | 0.818243 | 2.169692e-52 | 1.615289e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034674.01.T01 | MTR_8g098615 | 77.143 | 70 | 15 | 1 | 27 | 95 | 2 | 71 | 4.65e-35 | 115 |
| MsG0680034674.01.T01 | MTR_8g098615 | 81.250 | 64 | 11 | 1 | 33 | 95 | 1 | 64 | 1.28e-33 | 111 |
| MsG0680034674.01.T01 | MTR_8g098595 | 76.923 | 65 | 14 | 1 | 32 | 95 | 1 | 65 | 1.56e-31 | 108 |
| MsG0680034674.01.T01 | MTR_8g028920 | 64.865 | 74 | 25 | 1 | 23 | 95 | 7 | 80 | 6.20e-31 | 107 |
| MsG0680034674.01.T01 | MTR_8g098635 | 72.727 | 66 | 17 | 1 | 31 | 95 | 5 | 70 | 3.31e-27 | 102 |
| MsG0680034674.01.T01 | MTR_4g106690 | 60.811 | 74 | 28 | 1 | 23 | 95 | 7 | 80 | 3.95e-27 | 98.2 |
| MsG0680034674.01.T01 | MTR_1g073390 | 71.212 | 66 | 16 | 3 | 32 | 95 | 1 | 65 | 7.43e-26 | 92.0 |
| MsG0680034674.01.T01 | MTR_8g028920 | 76.316 | 38 | 8 | 1 | 59 | 95 | 4 | 41 | 1.45e-15 | 67.4 |
| MsG0680034674.01.T01 | MTR_1g073600 | 73.684 | 38 | 9 | 1 | 59 | 95 | 36 | 73 | 1.53e-13 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034674.01.T01 | AT1G63800 | 64.865 | 74 | 25 | 1 | 23 | 95 | 7 | 80 | 2.21e-29 | 103 |
| MsG0680034674.01.T01 | AT1G63800 | 70.769 | 65 | 18 | 1 | 32 | 95 | 1 | 65 | 6.09e-28 | 99.8 |
| MsG0680034674.01.T01 | AT5G41340 | 62.162 | 74 | 27 | 1 | 23 | 95 | 7 | 80 | 7.94e-28 | 100 |
| MsG0680034674.01.T01 | AT2G46030 | 54.795 | 73 | 32 | 1 | 23 | 94 | 7 | 79 | 2.52e-23 | 88.6 |
| MsG0680034674.01.T01 | AT2G46030 | 54.795 | 73 | 32 | 1 | 23 | 94 | 7 | 79 | 2.52e-23 | 88.6 |
| MsG0680034674.01.T01 | AT2G46030 | 58.209 | 67 | 27 | 1 | 29 | 94 | 16 | 82 | 5.93e-23 | 87.4 |
| MsG0680034674.01.T01 | AT2G46030 | 58.209 | 67 | 27 | 1 | 29 | 94 | 16 | 82 | 5.93e-23 | 87.4 |
| MsG0680034674.01.T01 | AT2G46030 | 54.795 | 73 | 32 | 1 | 23 | 94 | 79 | 151 | 5.98e-23 | 89.0 |
| MsG0680034674.01.T01 | AT5G41340 | 72.973 | 37 | 9 | 1 | 60 | 95 | 9 | 45 | 1.85e-13 | 62.4 |
Find 22 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATACAGCTTCTTGATA+TGG | 0.274256 | 6:+92622264 | None:intergenic |
| GTTAGAAACTTACTATGTTT+AGG | 0.347014 | 6:+92622954 | None:intergenic |
| ACTCACATTTCATCAACATT+AGG | 0.371368 | 6:+92622171 | None:intergenic |
| TTATCCTTATATGTCTCCTA+TGG | 0.389835 | 6:-92622217 | MsG0680034674.01.T01:CDS |
| GGAAGGAAGTTGTAACTAGA+AGG | 0.392743 | 6:-92623538 | MsG0680034674.01.T01:CDS |
| TATCCTTATATGTCTCCTAT+GGG | 0.430022 | 6:-92622216 | MsG0680034674.01.T01:CDS |
| GGAATTGAAGAGTTGAATCA+TGG | 0.456807 | 6:-92623560 | MsG0680034674.01.T01:CDS |
| AACCCCATAGGAGACATATA+AGG | 0.458071 | 6:+92622213 | None:intergenic |
| GTCCATATCAAGAAGCTGTA+TGG | 0.463848 | 6:-92622266 | MsG0680034674.01.T01:CDS |
| GACATATAAGGATAAACATC+AGG | 0.491233 | 6:+92622225 | None:intergenic |
| ATAAACAACAAGGTTATGAT+GGG | 0.574356 | 6:-92623593 | None:intergenic |
| GAAGGAAGTTGTAACTAGAA+GGG | 0.574954 | 6:-92623537 | MsG0680034674.01.T01:CDS |
| ATCCTTATATGTCTCCTATG+GGG | 0.581743 | 6:-92622215 | MsG0680034674.01.T01:CDS |
| ACATTTCATCAACATTAGGA+TGG | 0.594673 | 6:+92622175 | None:intergenic |
| GAATTGAAGAGTTGAATCAT+GGG | 0.598714 | 6:-92623559 | MsG0680034674.01.T01:CDS |
| AAGGAAGTTGTAACTAGAAG+GGG | 0.610781 | 6:-92623536 | MsG0680034674.01.T01:CDS |
| TGAAGAGTTGAATCATGGGA+AGG | 0.619958 | 6:-92623555 | MsG0680034674.01.T01:CDS |
| GTTATGATGGGACATGAAGA+CGG | 0.626752 | 6:-92623581 | MsG0680034674.01.T01:CDS |
| AGCTGTATGGAAGATAAGAG+TGG | 0.635714 | 6:-92622253 | MsG0680034674.01.T01:CDS |
| ACTTTGTTAACAAACCCCAT+AGG | 0.653809 | 6:+92622201 | None:intergenic |
| AAAGCGGAGATGATCAACAA+TGG | 0.687050 | 6:-92623008 | MsG0680034674.01.T01:CDS |
| GTTGATGACTGATTGCAAAG+CGG | 0.741293 | 6:-92623024 | MsG0680034674.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAACAATCTATAATACTAA+AGG | + | Chr6:92622913-92622932 | None:intergenic | 15.0% |
| !! | CATAAATAAACACAAATAAA+AGG | + | Chr6:92623282-92623301 | None:intergenic | 15.0% |
| !!! | ATTTCTAATCTTTTTGTAAT+TGG | - | Chr6:92622689-92622708 | MsG0680034674.01.T01:intron | 15.0% |
| !!! | TATGTTACATTTCTATTTTT+GGG | - | Chr6:92622361-92622380 | MsG0680034674.01.T01:intron | 15.0% |
| !!! | TTTCTAATCTTTTTGTAATT+GGG | - | Chr6:92622690-92622709 | MsG0680034674.01.T01:intron | 15.0% |
| !! | AGAAGAAAAAAATTGATACA+AGG | + | Chr6:92622826-92622845 | None:intergenic | 20.0% |
| !! | GAAGAAAAAAATTGATACAA+GGG | + | Chr6:92622825-92622844 | None:intergenic | 20.0% |
| !! | TAAAGGAATAACATTTCAAT+TGG | + | Chr6:92622896-92622915 | None:intergenic | 20.0% |
| !! | TATAATTACACTGAATTACA+TGG | + | Chr6:92623012-92623031 | None:intergenic | 20.0% |
| !!! | CAATTTTTTTCTTCTTTTTG+TGG | - | Chr6:92622831-92622850 | MsG0680034674.01.T01:intron | 20.0% |
| !!! | CTATGTTACATTTCTATTTT+TGG | - | Chr6:92622360-92622379 | MsG0680034674.01.T01:intron | 20.0% |
| !!! | CTATGTTTAATTTTGTTAAG+TGG | + | Chr6:92622498-92622517 | None:intergenic | 20.0% |
| ! | AAAGTGAAAACTTTCTCATT+TGG | - | Chr6:92622456-92622475 | MsG0680034674.01.T01:intron | 25.0% |
| ! | GTTAGAAACTTACTATGTTT+AGG | + | Chr6:92622800-92622819 | None:intergenic | 25.0% |
| ! | TTCAGTGTAATTATATGTGT+CGG | - | Chr6:92623017-92623036 | MsG0680034674.01.T01:CDS | 25.0% |
| !! | AATGAGAAAGTTTTCACTTT+TGG | + | Chr6:92622456-92622475 | None:intergenic | 25.0% |
| !! | TGGTTTTCTCAAATTATTTG+CGG | + | Chr6:92622992-92623011 | None:intergenic | 25.0% |
| !!! | TGTTATCACTCACTTTTATT+AGG | - | Chr6:92623451-92623470 | MsG0680034674.01.T01:intron | 25.0% |
| AACACAATTGTGACTTTAGA+GGG | - | Chr6:92623398-92623417 | MsG0680034674.01.T01:intron | 30.0% | |
| ACATTTCATCAACATTAGGA+TGG | + | Chr6:92623579-92623598 | None:intergenic | 30.0% | |
| ATAGGTTTCAATTCAATGTG+TGG | - | Chr6:92623102-92623121 | MsG0680034674.01.T01:intron | 30.0% | |
| ATGTTTCAATCTATGAAACG+CGG | - | Chr6:92622933-92622952 | MsG0680034674.01.T01:intron | 30.0% | |
| GAATTGAAGAGTTGAATCAT+GGG | - | Chr6:92622192-92622211 | MsG0680034674.01.T01:CDS | 30.0% | |
| GACATATAAGGATAAACATC+AGG | + | Chr6:92623529-92623548 | None:intergenic | 30.0% | |
| GGAAAATAAGCAAACAAGTA+AGG | + | Chr6:92623165-92623184 | None:intergenic | 30.0% | |
| TAACACAATTGTGACTTTAG+AGG | - | Chr6:92623397-92623416 | MsG0680034674.01.T01:intron | 30.0% | |
| TATCCTTATATGTCTCCTAT+GGG | - | Chr6:92623535-92623554 | MsG0680034674.01.T01:CDS | 30.0% | |
| TTATCCTTATATGTCTCCTA+TGG | - | Chr6:92623534-92623553 | MsG0680034674.01.T01:CDS | 30.0% | |
| !! | CGACAAAAAGTTATTTCTCT+TGG | + | Chr6:92623233-92623252 | None:intergenic | 30.0% |
| !! | TTTGCTTATTTTCCTCAATG+AGG | - | Chr6:92623171-92623190 | MsG0680034674.01.T01:intron | 30.0% |
| AAGGAAGTTGTAACTAGAAG+GGG | - | Chr6:92622215-92622234 | MsG0680034674.01.T01:CDS | 35.0% | |
| AATGCAATTCATCCTCATTG+AGG | + | Chr6:92623186-92623205 | None:intergenic | 35.0% | |
| ACTTTGTTAACAAACCCCAT+AGG | + | Chr6:92623553-92623572 | None:intergenic | 35.0% | |
| ATCCTTATATGTCTCCTATG+GGG | - | Chr6:92623536-92623555 | MsG0680034674.01.T01:CDS | 35.0% | |
| CAATGAGGATGAATTGCATT+AGG | - | Chr6:92623186-92623205 | MsG0680034674.01.T01:intron | 35.0% | |
| GAAACATATCTTGTTGAGAG+AGG | - | Chr6:92622281-92622300 | MsG0680034674.01.T01:intron | 35.0% | |
| GAAGGAAGTTGTAACTAGAA+GGG | - | Chr6:92622214-92622233 | MsG0680034674.01.T01:CDS | 35.0% | |
| GAATTGAAACCTATGAAGCA+TGG | + | Chr6:92623096-92623115 | None:intergenic | 35.0% | |
| GGAATTGAAGAGTTGAATCA+TGG | - | Chr6:92622191-92622210 | MsG0680034674.01.T01:CDS | 35.0% | |
| TCTCAAATTATTTGCGGAGT+CGG | + | Chr6:92622986-92623005 | None:intergenic | 35.0% | |
| TTCCATACAGCTTCTTGATA+TGG | + | Chr6:92623490-92623509 | None:intergenic | 35.0% | |
| ! | ACACAATTGTGACTTTAGAG+GGG | - | Chr6:92623399-92623418 | MsG0680034674.01.T01:intron | 35.0% |
| ! | CTTTTATTAGGACTCATTGC+AGG | - | Chr6:92623463-92623482 | MsG0680034674.01.T01:intron | 35.0% |
| ! | GTAATTATATGTGTCGGTGT+CGG | - | Chr6:92623023-92623042 | MsG0680034674.01.T01:CDS | 35.0% |
| ! | TAATTGGGTTGTTGTGAATC+AGG | - | Chr6:92622705-92622724 | MsG0680034674.01.T01:intron | 35.0% |
| ! | TTCTGCTTGTGCTTCTATTT+TGG | - | Chr6:92622527-92622546 | MsG0680034674.01.T01:intron | 35.0% |
| AAAGCGGAGATGATCAACAA+TGG | - | Chr6:92622743-92622762 | MsG0680034674.01.T01:intron | 40.0% | |
| AAATGTGCGTATGTTGCAGA+AGG | - | Chr6:92623302-92623321 | MsG0680034674.01.T01:intron | 40.0% | |
| AACCCCATAGGAGACATATA+AGG | + | Chr6:92623541-92623560 | None:intergenic | 40.0% | |
| AGCTGTATGGAAGATAAGAG+TGG | - | Chr6:92623498-92623517 | MsG0680034674.01.T01:intron | 40.0% | |
| GATTAGACGTGTTTCAGTGT+CGG | + | Chr6:92623063-92623082 | None:intergenic | 40.0% | |
| GGAAGGAAGTTGTAACTAGA+AGG | - | Chr6:92622213-92622232 | MsG0680034674.01.T01:CDS | 40.0% | |
| GTCCATATCAAGAAGCTGTA+TGG | - | Chr6:92623485-92623504 | MsG0680034674.01.T01:intron | 40.0% | |
| TATGAAGCATGGACACTTCT+AGG | + | Chr6:92623085-92623104 | None:intergenic | 40.0% | |
| TGAAGAGTTGAATCATGGGA+AGG | - | Chr6:92622196-92622215 | MsG0680034674.01.T01:CDS | 40.0% | |
| TGCTATCTTTGCACAGTATG+AGG | + | Chr6:92622582-92622601 | None:intergenic | 40.0% | |
| ! | CACAATTGTGACTTTAGAGG+GGG | - | Chr6:92623400-92623419 | MsG0680034674.01.T01:intron | 40.0% |
| !! | GTTGATGACTGATTGCAAAG+CGG | - | Chr6:92622727-92622746 | MsG0680034674.01.T01:intron | 40.0% |
| !! | TAGAAGTGTCCATGCTTCAT+AGG | - | Chr6:92623084-92623103 | MsG0680034674.01.T01:intron | 40.0% |
| CTTCGTCACTTCTCTTTCGT+TGG | + | Chr6:92622621-92622640 | None:intergenic | 45.0% | |
| TTTAGAGGGGGAACTGAAGT+GGG | - | Chr6:92623412-92623431 | MsG0680034674.01.T01:intron | 45.0% | |
| CTTGTTGAGAGAGGAAGACG+AGG | - | Chr6:92622290-92622309 | MsG0680034674.01.T01:intron | 50.0% | |
| TATCAGTTCCGGTGTCTGTG+CGG | + | Chr6:92622963-92622982 | None:intergenic | 50.0% | |
| ! | CTTTAGAGGGGGAACTGAAG+TGG | - | Chr6:92623411-92623430 | MsG0680034674.01.T01:intron | 50.0% |
| ATCAGTTCCGGTGTCTGTGC+GGG | + | Chr6:92622962-92622981 | None:intergenic | 55.0% | |
| TGCGGAGTCGGTATCAGTTC+CGG | + | Chr6:92622974-92622993 | None:intergenic | 55.0% | |
| GCGGATACCCGCACAGACAC+CGG | - | Chr6:92622952-92622971 | MsG0680034674.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 92622173 | 92623600 | 92622173 | ID=MsG0680034674.01;Name=MsG0680034674.01 |
| Chr6 | mRNA | 92622173 | 92623600 | 92622173 | ID=MsG0680034674.01.T01;Parent=MsG0680034674.01;Name=MsG0680034674.01.T01;_AED=0.65;_eAED=0.65;_QI=0|0|0|0.66|1|1|3|0|95 |
| Chr6 | exon | 92623509 | 92623600 | 92623509 | ID=MsG0680034674.01.T01:exon:12884;Parent=MsG0680034674.01.T01 |
| Chr6 | exon | 92622967 | 92623046 | 92622967 | ID=MsG0680034674.01.T01:exon:12883;Parent=MsG0680034674.01.T01 |
| Chr6 | exon | 92622173 | 92622288 | 92622173 | ID=MsG0680034674.01.T01:exon:12882;Parent=MsG0680034674.01.T01 |
| Chr6 | CDS | 92623509 | 92623600 | 92623509 | ID=MsG0680034674.01.T01:cds;Parent=MsG0680034674.01.T01 |
| Chr6 | CDS | 92622967 | 92623046 | 92622967 | ID=MsG0680034674.01.T01:cds;Parent=MsG0680034674.01.T01 |
| Chr6 | CDS | 92622173 | 92622288 | 92622173 | ID=MsG0680034674.01.T01:cds;Parent=MsG0680034674.01.T01 |
| Gene Sequence |
| Protein sequence |
