Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035463.01.T01 | XP_003620440.1 | 93.78 | 209 | 8 | 2 | 1 | 205 | 1 | 208 | 1.06E-135 | 389 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035463.01.T01 | G7KMZ9 | 93.780 | 209 | 8 | 2 | 1 | 205 | 1 | 208 | 5.06e-136 | 389 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001911.01 | MsG0680035463.01 | 0.808186 | 3.505135e-50 | 1.990984e-47 |
| MsG0180003984.01 | MsG0680035463.01 | 0.817359 | 3.436103e-52 | 2.496902e-49 |
| MsG0180004160.01 | MsG0680035463.01 | 0.817439 | 3.296282e-52 | 2.400626e-49 |
| MsG0180004272.01 | MsG0680035463.01 | 0.810001 | 1.431844e-50 | 8.533124e-48 |
| MsG0180004991.01 | MsG0680035463.01 | 0.826146 | 3.183645e-54 | 2.960949e-51 |
| MsG0180005451.01 | MsG0680035463.01 | 0.805821 | 1.109074e-49 | 5.925868e-47 |
| MsG0680035463.01 | MsG0780040519.01 | 0.809675 | 1.682696e-50 | 9.942482e-48 |
| MsG0680035463.01 | MsG0780041423.01 | 0.837124 | 6.256039e-57 | 8.048102e-54 |
| MsG0480020917.01 | MsG0680035463.01 | 0.824351 | 8.459115e-54 | 7.473734e-51 |
| MsG0480023240.01 | MsG0680035463.01 | 0.806353 | 8.571909e-50 | 4.642445e-47 |
| MsG0380017431.01 | MsG0680035463.01 | 0.804109 | 2.528775e-49 | 1.292892e-46 |
| MsG0580029588.01 | MsG0680035463.01 | 0.820219 | 7.699368e-53 | 6.055200e-50 |
| MsG0280006728.01 | MsG0680035463.01 | 0.802607 | 5.178809e-49 | 2.547711e-46 |
| MsG0280006881.01 | MsG0680035463.01 | 0.819301 | 1.248111e-52 | 9.567281e-50 |
| MsG0280007440.01 | MsG0680035463.01 | 0.813231 | 2.841521e-51 | 1.845104e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035463.01.T01 | MTR_6g083920 | 93.780 | 209 | 8 | 2 | 1 | 205 | 1 | 208 | 1.28e-139 | 389 |
| MsG0680035463.01.T01 | MTR_1g110370 | 35.714 | 224 | 92 | 8 | 2 | 205 | 20 | 211 | 2.71e-27 | 104 |
| MsG0680035463.01.T01 | MTR_6g008660 | 31.441 | 229 | 98 | 11 | 2 | 205 | 24 | 218 | 3.88e-21 | 88.6 |
| MsG0680035463.01.T01 | MTR_5g056040 | 30.131 | 229 | 105 | 6 | 4 | 204 | 23 | 224 | 3.94e-19 | 83.2 |
| MsG0680035463.01.T01 | MTR_1g110360 | 27.962 | 211 | 94 | 7 | 8 | 194 | 33 | 209 | 3.88e-18 | 80.1 |
| MsG0680035463.01.T01 | MTR_4g100570 | 26.667 | 225 | 125 | 7 | 5 | 204 | 9 | 218 | 2.13e-14 | 70.1 |
| MsG0680035463.01.T01 | MTR_5g083820 | 29.279 | 222 | 99 | 7 | 8 | 205 | 31 | 218 | 4.40e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035463.01.T01 | AT1G68340 | 33.475 | 236 | 102 | 9 | 4 | 206 | 8 | 221 | 9.08e-27 | 102 |
| MsG0680035463.01.T01 | AT1G68340 | 32.766 | 235 | 103 | 9 | 4 | 205 | 8 | 220 | 8.81e-26 | 100 |
| MsG0680035463.01.T01 | AT1G68340 | 32.766 | 235 | 103 | 9 | 4 | 205 | 8 | 220 | 9.00e-26 | 100 |
| MsG0680035463.01.T01 | AT3G18295 | 39.901 | 203 | 105 | 5 | 3 | 205 | 7 | 192 | 5.28e-23 | 92.4 |
| MsG0680035463.01.T01 | AT1G25370 | 28.279 | 244 | 116 | 5 | 4 | 204 | 16 | 243 | 9.59e-19 | 82.0 |
| MsG0680035463.01.T01 | AT1G25370 | 28.279 | 244 | 116 | 5 | 4 | 204 | 16 | 243 | 9.59e-19 | 82.0 |
| MsG0680035463.01.T01 | AT1G48770 | 32.692 | 208 | 88 | 5 | 1 | 205 | 1 | 159 | 1.26e-15 | 72.0 |
| MsG0680035463.01.T01 | AT1G48770 | 32.692 | 208 | 88 | 5 | 1 | 205 | 1 | 159 | 1.26e-15 | 72.0 |
| MsG0680035463.01.T01 | AT1G48770 | 32.692 | 208 | 88 | 5 | 1 | 205 | 1 | 159 | 1.26e-15 | 72.0 |
Find 37 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTCAATCTTTGAAGAAAA+TGG | 0.226034 | 6:+106200203 | MsG0680035463.01.T01:CDS |
| TGACACATCTTAGTGTTCTT+TGG | 0.298065 | 6:-106200076 | None:intergenic |
| TTTCATTCCCATGTTTGAAA+TGG | 0.313234 | 6:+106200048 | MsG0680035463.01.T01:CDS |
| TTCATTCCCATGTTTGAAAT+GGG | 0.340857 | 6:+106200049 | MsG0680035463.01.T01:CDS |
| GATGATGAAGAAGAAGAAAA+TGG | 0.381648 | 6:+106200466 | MsG0680035463.01.T01:CDS |
| ATTCTAATGCTGTTAGGCCT+TGG | 0.418513 | 6:+106200312 | MsG0680035463.01.T01:CDS |
| TGTTTCTCTTTCTAAGGAAG+AGG | 0.443092 | 6:+106200517 | MsG0680035463.01.T01:CDS |
| GGCGATGCCGTCGTCGGTAC+CGG | 0.449421 | 6:+106200379 | MsG0680035463.01.T01:CDS |
| TTCTTCTTCTTCATCATCAA+AGG | 0.459416 | 6:-106200461 | None:intergenic |
| TCTTTGACGAACAACATCAA+TGG | 0.466334 | 6:-106200141 | None:intergenic |
| ACCATTGCCAACAACATCAT+TGG | 0.473245 | 6:-106200288 | None:intergenic |
| ATGGTGATTCTAATGCTGTT+AGG | 0.480885 | 6:+106200306 | MsG0680035463.01.T01:CDS |
| GTTGTTGCCAATGATGTTGT+TGG | 0.490407 | 6:+106200281 | MsG0680035463.01.T01:CDS |
| CTTCTTGTTCTCAAATTCCA+AGG | 0.493657 | 6:-106200329 | None:intergenic |
| GTACCGGTGGTGAAAGATGA+AGG | 0.503918 | 6:+106200395 | MsG0680035463.01.T01:CDS |
| CTCCCTTCATCTTTCACCAC+CGG | 0.510421 | 6:-106200398 | None:intergenic |
| TGTTGTTCGTCAAAGAATCA+TGG | 0.518717 | 6:+106200148 | MsG0680035463.01.T01:CDS |
| GCCAATGATGTTGTTGGCAA+TGG | 0.522875 | 6:+106200287 | MsG0680035463.01.T01:CDS |
| TTGGGACTAGACCACCTAGA+AGG | 0.530301 | 6:+106200564 | MsG0680035463.01.T01:CDS |
| ATGTAAAGCACCACTGGTAC+CGG | 0.530517 | 6:+106200358 | MsG0680035463.01.T01:CDS |
| TGGTTACCCCATTTCAAACA+TGG | 0.539045 | 6:-106200056 | None:intergenic |
| ATCATCAAAGGTGAAGAAGA+AGG | 0.545431 | 6:-106200449 | None:intergenic |
| AGCTGCATGTAAAGCACCAC+TGG | 0.548134 | 6:+106200352 | MsG0680035463.01.T01:CDS |
| AATGCTCATGTTGGTCATGT+TGG | 0.554010 | 6:+106200251 | MsG0680035463.01.T01:CDS |
| GGTTACCCCATTTCAAACAT+GGG | 0.555356 | 6:-106200055 | None:intergenic |
| GGTACCGACGACGGCATCGC+CGG | 0.576685 | 6:-106200377 | None:intergenic |
| GACGGCATCGCCGGTACCAG+TGG | 0.577732 | 6:-106200368 | None:intergenic |
| TCATTCCCATGTTTGAAATG+GGG | 0.586906 | 6:+106200050 | MsG0680035463.01.T01:CDS |
| TGGTGAAAGATGAAGGGAGG+AGG | 0.602930 | 6:+106200402 | MsG0680035463.01.T01:CDS |
| ACAAATGTTAATGCTCATGT+TGG | 0.608142 | 6:+106200242 | MsG0680035463.01.T01:CDS |
| GATGCCGTCGTCGGTACCGG+TGG | 0.618149 | 6:+106200382 | MsG0680035463.01.T01:CDS |
| GTTGTTCGTCAAAGAATCAT+GGG | 0.629243 | 6:+106200149 | MsG0680035463.01.T01:CDS |
| TTCACCACCGGTACCGACGA+CGG | 0.638456 | 6:-106200386 | None:intergenic |
| GGGAATGAAAAGTTGTGTAG+TGG | 0.642191 | 6:-106200035 | None:intergenic |
| GGTACCGGCGATGCCGTCGT+CGG | 0.647045 | 6:+106200373 | MsG0680035463.01.T01:CDS |
| TACCGGTGGTGAAAGATGAA+GGG | 0.647536 | 6:+106200396 | MsG0680035463.01.T01:CDS |
| CGGTGGTGAAAGATGAAGGG+AGG | 0.665743 | 6:+106200399 | MsG0680035463.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTGAAACTTTAAGCTTTT+TGG | - | Chr6:106200192-106200211 | None:intergenic | 25.0% |
| !! | GTTTCAATCTTTGAAGAAAA+TGG | + | Chr6:106200203-106200222 | MsG0680035463.01.T01:CDS | 25.0% |
| !!! | AATGGATTCATTGTTTTTGT+TGG | - | Chr6:106200126-106200145 | None:intergenic | 25.0% |
| !!! | ATTTTCTGTTTCTCTTTCTA+AGG | + | Chr6:106200511-106200530 | MsG0680035463.01.T01:CDS | 25.0% |
| ACAAATGTTAATGCTCATGT+TGG | + | Chr6:106200242-106200261 | MsG0680035463.01.T01:CDS | 30.0% | |
| GATGATGAAGAAGAAGAAAA+TGG | + | Chr6:106200466-106200485 | MsG0680035463.01.T01:CDS | 30.0% | |
| TTCTTCTTCTTCATCATCAA+AGG | - | Chr6:106200464-106200483 | None:intergenic | 30.0% | |
| TTTCATTCCCATGTTTGAAA+TGG | + | Chr6:106200048-106200067 | MsG0680035463.01.T01:CDS | 30.0% | |
| ! | AAGAGGTTGAAGAAGATTTT+TGG | + | Chr6:106200534-106200553 | MsG0680035463.01.T01:CDS | 30.0% |
| ! | ACAATCCTAGATCTCTTTTT+AGG | - | Chr6:106200590-106200609 | None:intergenic | 30.0% |
| ! | AGAGGTTGAAGAAGATTTTT+GGG | + | Chr6:106200535-106200554 | MsG0680035463.01.T01:CDS | 30.0% |
| ! | GTTTTTGTTGGAATTGTTGT+TGG | - | Chr6:106200114-106200133 | None:intergenic | 30.0% |
| ! | TTCATTCCCATGTTTGAAAT+GGG | + | Chr6:106200049-106200068 | MsG0680035463.01.T01:CDS | 30.0% |
| ATCATCAAAGGTGAAGAAGA+AGG | - | Chr6:106200452-106200471 | None:intergenic | 35.0% | |
| ATGGTGATTCTAATGCTGTT+AGG | + | Chr6:106200306-106200325 | MsG0680035463.01.T01:CDS | 35.0% | |
| CTTCTTGTTCTCAAATTCCA+AGG | - | Chr6:106200332-106200351 | None:intergenic | 35.0% | |
| GTTGTTCGTCAAAGAATCAT+GGG | + | Chr6:106200149-106200168 | MsG0680035463.01.T01:CDS | 35.0% | |
| TCTTTGACGAACAACATCAA+TGG | - | Chr6:106200144-106200163 | None:intergenic | 35.0% | |
| TGACACATCTTAGTGTTCTT+TGG | - | Chr6:106200079-106200098 | None:intergenic | 35.0% | |
| TGTTGTTCGTCAAAGAATCA+TGG | + | Chr6:106200148-106200167 | MsG0680035463.01.T01:CDS | 35.0% | |
| ! | TCATTCCCATGTTTGAAATG+GGG | + | Chr6:106200050-106200069 | MsG0680035463.01.T01:CDS | 35.0% |
| ! | TGTTTCTCTTTCTAAGGAAG+AGG | + | Chr6:106200517-106200536 | MsG0680035463.01.T01:CDS | 35.0% |
| !! | AAGATTTTTGGGCTTTGGTT+GGG | + | Chr6:106200546-106200565 | MsG0680035463.01.T01:CDS | 35.0% |
| !! | TGAAGAAGATTTTTGGGCTT+TGG | + | Chr6:106200541-106200560 | MsG0680035463.01.T01:CDS | 35.0% |
| !!! | TGTAGTGGTTTTGATCTTTC+TGG | - | Chr6:106200023-106200042 | None:intergenic | 35.0% |
| AATGCTCATGTTGGTCATGT+TGG | + | Chr6:106200251-106200270 | MsG0680035463.01.T01:CDS | 40.0% | |
| ACCATTGCCAACAACATCAT+TGG | - | Chr6:106200291-106200310 | None:intergenic | 40.0% | |
| ATTCTAATGCTGTTAGGCCT+TGG | + | Chr6:106200312-106200331 | MsG0680035463.01.T01:CDS | 40.0% | |
| GAAGGCCTAAAAAGAGATCT+AGG | + | Chr6:106200582-106200601 | MsG0680035463.01.T01:CDS | 40.0% | |
| GGGAATGAAAAGTTGTGTAG+TGG | - | Chr6:106200038-106200057 | None:intergenic | 40.0% | |
| GGTTACCCCATTTCAAACAT+GGG | - | Chr6:106200058-106200077 | None:intergenic | 40.0% | |
| GTTGTTGCCAATGATGTTGT+TGG | + | Chr6:106200281-106200300 | MsG0680035463.01.T01:CDS | 40.0% | |
| TGGTTACCCCATTTCAAACA+TGG | - | Chr6:106200059-106200078 | None:intergenic | 40.0% | |
| !! | GAAGATTTTTGGGCTTTGGT+TGG | + | Chr6:106200545-106200564 | MsG0680035463.01.T01:CDS | 40.0% |
| !! | GATCTCTTTTTAGGCCTTCT+AGG | - | Chr6:106200581-106200600 | None:intergenic | 40.0% |
| GCCAATGATGTTGTTGGCAA+TGG | + | Chr6:106200287-106200306 | MsG0680035463.01.T01:CDS | 45.0% | |
| TACCGGTGGTGAAAGATGAA+GGG | + | Chr6:106200396-106200415 | MsG0680035463.01.T01:CDS | 45.0% | |
| ! | ATGTAAAGCACCACTGGTAC+CGG | + | Chr6:106200358-106200377 | MsG0680035463.01.T01:CDS | 45.0% |
| !! | CTCTTTTTAGGCCTTCTAGG+TGG | - | Chr6:106200578-106200597 | None:intergenic | 45.0% |
| !!! | AGGGAGGAGGTTTTTTGATG+TGG | + | Chr6:106200415-106200434 | MsG0680035463.01.T01:CDS | 45.0% |
| !!! | GGAGGAGGTTTTTTGATGTG+GGG | + | Chr6:106200417-106200436 | MsG0680035463.01.T01:CDS | 45.0% |
| !!! | GGGAGGAGGTTTTTTGATGT+GGG | + | Chr6:106200416-106200435 | MsG0680035463.01.T01:CDS | 45.0% |
| CTCCCTTCATCTTTCACCAC+CGG | - | Chr6:106200401-106200420 | None:intergenic | 50.0% | |
| TGGTGAAAGATGAAGGGAGG+AGG | + | Chr6:106200402-106200421 | MsG0680035463.01.T01:CDS | 50.0% | |
| TTGGGACTAGACCACCTAGA+AGG | + | Chr6:106200564-106200583 | MsG0680035463.01.T01:CDS | 50.0% | |
| ! | AGCTGCATGTAAAGCACCAC+TGG | + | Chr6:106200352-106200371 | MsG0680035463.01.T01:CDS | 50.0% |
| ! | GTACCGGTGGTGAAAGATGA+AGG | + | Chr6:106200395-106200414 | MsG0680035463.01.T01:CDS | 50.0% |
| CGGTGGTGAAAGATGAAGGG+AGG | + | Chr6:106200399-106200418 | MsG0680035463.01.T01:CDS | 55.0% | |
| !! | TTCACCACCGGTACCGACGA+CGG | - | Chr6:106200389-106200408 | None:intergenic | 60.0% |
| GACGGCATCGCCGGTACCAG+TGG | - | Chr6:106200371-106200390 | None:intergenic | 70.0% | |
| GGCGATGCCGTCGTCGGTAC+CGG | + | Chr6:106200379-106200398 | MsG0680035463.01.T01:CDS | 70.0% | |
| ! | GATGCCGTCGTCGGTACCGG+TGG | + | Chr6:106200382-106200401 | MsG0680035463.01.T01:CDS | 70.0% |
| !! | GGTACCGACGACGGCATCGC+CGG | - | Chr6:106200380-106200399 | None:intergenic | 70.0% |
| !! | GGTACCGGCGATGCCGTCGT+CGG | + | Chr6:106200373-106200392 | MsG0680035463.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 106200014 | 106200637 | 106200014 | ID=MsG0680035463.01;Name=MsG0680035463.01 |
| Chr6 | mRNA | 106200014 | 106200637 | 106200014 | ID=MsG0680035463.01.T01;Parent=MsG0680035463.01;Name=MsG0680035463.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|207 |
| Chr6 | exon | 106200014 | 106200637 | 106200014 | ID=MsG0680035463.01.T01:exon:11394;Parent=MsG0680035463.01.T01 |
| Chr6 | CDS | 106200014 | 106200637 | 106200014 | ID=MsG0680035463.01.T01:cds;Parent=MsG0680035463.01.T01 |
| Gene Sequence |
| Protein sequence |
