Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002891.01 | MsG0680035623.01 | 0.830491 | 2.850612e-55 | 3.007793e-52 |
| MsG0180005338.01 | MsG0680035623.01 | 0.804689 | 1.914519e-49 | 9.935757e-47 |
| MsG0380012931.01 | MsG0680035623.01 | 0.807367 | 5.232101e-50 | 2.908794e-47 |
| MsG0680032002.01 | MsG0680035623.01 | 0.807836 | 4.160102e-50 | 2.341245e-47 |
| MsG0680032512.01 | MsG0680035623.01 | 0.881813 | 1.757359e-70 | 1.044374e-66 |
| MsG0680035623.01 | MsG0880046969.01 | 0.805528 | 1.277991e-49 | 6.776753e-47 |
| MsG0480021892.01 | MsG0680035623.01 | 0.805142 | 1.539374e-49 | 8.082611e-47 |
| MsG0280006533.01 | MsG0680035623.01 | 0.801501 | 8.741197e-49 | 4.181171e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGAACACGATGAGAAATTT+TGG | 0.186460 | 6:+109502099 | None:intergenic |
| GGATGGGGATGGAATTGATT+TGG | 0.326004 | 6:+109502018 | None:intergenic |
| TGGTTTAAGACGGTTGTTGC+AGG | 0.360772 | 6:+109501948 | None:intergenic |
| GATGGGGATGGAATTGATTT+GGG | 0.361364 | 6:+109502019 | None:intergenic |
| GTTGTGAGTTCTATGTGTGC+TGG | 0.419930 | 6:+109501996 | None:intergenic |
| AGTTCTATGTGTGCTGGGAT+GGG | 0.447786 | 6:+109502002 | None:intergenic |
| GAGTTCTATGTGTGCTGGGA+TGG | 0.474824 | 6:+109502001 | None:intergenic |
| GTTCTATGTGTGCTGGGATG+GGG | 0.492712 | 6:+109502003 | None:intergenic |
| ACACGCGCCGGCGAATTTGA+CGG | 0.494053 | 6:+109501896 | None:intergenic |
| ACAACCTACGGTTTCCCAAT+CGG | 0.501786 | 6:-109501978 | MsG0680035623.01.T01:CDS |
| TATGTGTGCTGGGATGGGGA+TGG | 0.518267 | 6:+109502007 | None:intergenic |
| CACTTCATCAACAAAACCTC+CGG | 0.519364 | 6:-109501927 | MsG0680035623.01.T01:CDS |
| TTGTGAGTTCTATGTGTGCT+GGG | 0.538462 | 6:+109501997 | None:intergenic |
| TTGCAGGGAGAAGACCGATT+GGG | 0.540559 | 6:+109501964 | None:intergenic |
| CACATAGAACTCACAACCTA+CGG | 0.571226 | 6:-109501990 | MsG0680035623.01.T01:CDS |
| GGTTTAAGACGGTTGTTGCA+GGG | 0.573978 | 6:+109501949 | None:intergenic |
| GAATTTGACGGAGAATTCGC+CGG | 0.577056 | 6:+109501908 | None:intergenic |
| GTTGATGAAGTGGTTTAAGA+CGG | 0.583505 | 6:+109501938 | None:intergenic |
| GAATTCTCCGTCAAATTCGC+CGG | 0.588379 | 6:-109501903 | MsG0680035623.01.T01:CDS |
| GTTGCAGGGAGAAGACCGAT+TGG | 0.601695 | 6:+109501963 | None:intergenic |
| AAGACCGATTGGGAAACCGT+AGG | 0.704857 | 6:+109501974 | None:intergenic |
| TTTGACGGAGAATTCGCCGG+AGG | 0.706889 | 6:+109501911 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GGAATTGATTTGGGATTTTA+GGG | + | Chr6:109501974-109501993 | None:intergenic | 30.0% |
| !!! | TGGAATTGATTTGGGATTTT+AGG | + | Chr6:109501975-109501994 | None:intergenic | 30.0% |
| ! | ACGAACACGATGAGAAATTT+TGG | + | Chr6:109501903-109501922 | None:intergenic | 35.0% |
| ! | GTTGATGAAGTGGTTTAAGA+CGG | + | Chr6:109502064-109502083 | None:intergenic | 35.0% |
| !!! | GATTTGGGATTTTAGGGTTA+GGG | + | Chr6:109501968-109501987 | None:intergenic | 35.0% |
| !!! | TGATTTGGGATTTTAGGGTT+AGG | + | Chr6:109501969-109501988 | None:intergenic | 35.0% |
| CACATAGAACTCACAACCTA+CGG | - | Chr6:109502009-109502028 | MsG0680035623.01.T01:CDS | 40.0% | |
| CACTTCATCAACAAAACCTC+CGG | - | Chr6:109502072-109502091 | MsG0680035623.01.T01:CDS | 40.0% | |
| TTGTGAGTTCTATGTGTGCT+GGG | + | Chr6:109502005-109502024 | None:intergenic | 40.0% | |
| ! | GATGGGGATGGAATTGATTT+GGG | + | Chr6:109501983-109502002 | None:intergenic | 40.0% |
| !!! | GGATTTTAGGGTTAGGGTTA+GGG | + | Chr6:109501962-109501981 | None:intergenic | 40.0% |
| ACAACCTACGGTTTCCCAAT+CGG | - | Chr6:109502021-109502040 | MsG0680035623.01.T01:CDS | 45.0% | |
| AGTTCTATGTGTGCTGGGAT+GGG | + | Chr6:109502000-109502019 | None:intergenic | 45.0% | |
| GAATTCTCCGTCAAATTCGC+CGG | - | Chr6:109502096-109502115 | MsG0680035623.01.T01:CDS | 45.0% | |
| GAATTTGACGGAGAATTCGC+CGG | + | Chr6:109502094-109502113 | None:intergenic | 45.0% | |
| GGATGGGGATGGAATTGATT+TGG | + | Chr6:109501984-109502003 | None:intergenic | 45.0% | |
| GTTGTGAGTTCTATGTGTGC+TGG | + | Chr6:109502006-109502025 | None:intergenic | 45.0% | |
| ! | GGTTTAAGACGGTTGTTGCA+GGG | + | Chr6:109502053-109502072 | None:intergenic | 45.0% |
| ! | TGGTTTAAGACGGTTGTTGC+AGG | + | Chr6:109502054-109502073 | None:intergenic | 45.0% |
| !!! | CGGAGGTTTTGTTGATGAAG+TGG | + | Chr6:109502074-109502093 | None:intergenic | 45.0% |
| !!! | GGGATTTTAGGGTTAGGGTT+AGG | + | Chr6:109501963-109501982 | None:intergenic | 45.0% |
| AAGACCGATTGGGAAACCGT+AGG | + | Chr6:109502028-109502047 | None:intergenic | 50.0% | |
| GAGTTCTATGTGTGCTGGGA+TGG | + | Chr6:109502001-109502020 | None:intergenic | 50.0% | |
| GTTCTATGTGTGCTGGGATG+GGG | + | Chr6:109501999-109502018 | None:intergenic | 50.0% | |
| TTGCAGGGAGAAGACCGATT+GGG | + | Chr6:109502038-109502057 | None:intergenic | 50.0% | |
| GTTGCAGGGAGAAGACCGAT+TGG | + | Chr6:109502039-109502058 | None:intergenic | 55.0% | |
| TATGTGTGCTGGGATGGGGA+TGG | + | Chr6:109501995-109502014 | None:intergenic | 55.0% | |
| TTTGACGGAGAATTCGCCGG+AGG | + | Chr6:109502091-109502110 | None:intergenic | 55.0% | |
| ! | ACACGCGCCGGCGAATTTGA+CGG | + | Chr6:109502106-109502125 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 109501894 | 109502127 | 109501894 | ID=MsG0680035623.01;Name=MsG0680035623.01 |
| Chr6 | mRNA | 109501894 | 109502127 | 109501894 | ID=MsG0680035623.01.T01;Parent=MsG0680035623.01;Name=MsG0680035623.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|77 |
| Chr6 | exon | 109501894 | 109502127 | 109501894 | ID=MsG0680035623.01.T01:exon:8612;Parent=MsG0680035623.01.T01 |
| Chr6 | CDS | 109501894 | 109502127 | 109501894 | ID=MsG0680035623.01.T01:cds;Parent=MsG0680035623.01.T01 |
| Gene Sequence |
| Protein sequence |
