AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005338.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005338.01.T01 MTR_1g097430 97.590 83 2 0 65 147 41 123 1.06e-51 163
MsG0180005338.01.T01 MTR_1g097430 97.590 83 2 0 65 147 85 167 3.71e-51 163
MsG0180005338.01.T01 MTR_1g097430 97.590 83 2 0 65 147 85 167 6.53e-51 163
MsG0180005338.01.T01 MTR_1g097430 88.043 92 2 1 65 147 85 176 4.47e-48 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005338.01.T01 AT1G22750 54.217 83 38 0 65 147 87 169 5.36e-22 88.2
MsG0180005338.01.T01 AT1G22750 54.217 83 38 0 65 147 87 169 5.79e-22 88.2
MsG0180005338.01.T01 AT1G22750 54.217 83 38 0 65 147 87 169 6.51e-22 88.2
MsG0180005338.01.T01 AT1G22750 54.217 83 38 0 65 147 87 169 7.12e-22 88.2

Find 18 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAAAATATGATGGAAAAT+TGG 0.236549 1:+90518081 None:intergenic
ATTGGGTCAGCTCGATTCTT+TGG 0.271091 1:+90518544 MsG0180005338.01.T01:CDS
TCTTGTCTAGGATCGTTTAA+AGG 0.271904 1:+90519333 MsG0180005338.01.T01:intron
TTATTTACAAGGAAGCAAAT+TGG 0.370778 1:+90518526 MsG0180005338.01.T01:CDS
TATTTACAAGGAAGCAAATT+GGG 0.429007 1:+90518527 MsG0180005338.01.T01:CDS
CAACACCCAAAATGCTGAAA+AGG 0.432749 1:-90519779 None:intergenic
TGTTGTCATGCTTGGAACCT+TGG 0.450047 1:+90519797 MsG0180005338.01.T01:CDS
CCAGGCCTGGGTTATTTACA+AGG 0.474179 1:+90518515 MsG0180005338.01.T01:intron
TGATGGAAAATTGGCTTAGG+TGG 0.488862 1:+90518090 MsG0180005338.01.T01:CDS
GTATACACTCTTGTCACTGA+TGG 0.490061 1:+90519819 MsG0180005338.01.T01:CDS
ATATGATGGAAAATTGGCTT+AGG 0.498442 1:+90518087 None:intergenic
CGCAATAGAACATAATACAT+AGG 0.530191 1:-90519478 None:intergenic
TGCTTCCTTGTAAATAACCC+AGG 0.554220 1:-90518520 None:intergenic
GCAATAGAACATAATACATA+GGG 0.563470 1:-90519477 None:intergenic
GTCAATAGCTCTGTACGGAA+AGG 0.603621 1:-90519846 None:intergenic
ACGGAGTCAATAGCTCTGTA+CGG 0.618927 1:-90519851 None:intergenic
GTCATCACAAGAGTCTTCAC+AGG 0.620790 1:+90519453 MsG0180005338.01.T01:CDS
AGTGACAAGAGTGTATACCA+AGG 0.744399 1:-90519814 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTAATTTTAAATAT+AGG + Chr1:90518875-90518894 MsG0180005338.01.T01:intron 0.0%
!! TAATGTTCCATAAATATATT+TGG + Chr1:90519575-90519594 MsG0180005338.01.T01:intron 15.0%
!! TACTTAATTCTTACTTATAT+TGG + Chr1:90519100-90519119 MsG0180005338.01.T01:intron 15.0%
!! TATAAATTGGAAAATATCAA+AGG - Chr1:90518733-90518752 None:intergenic 15.0%
!! ACATTAAACATAAAATCATG+TGG - Chr1:90519660-90519679 None:intergenic 20.0%
!! ATTTAGATAATCCTATTTCA+TGG + Chr1:90519617-90519636 MsG0180005338.01.T01:intron 20.0%
!! CATAAATATATTTGGAGAAA+AGG + Chr1:90519583-90519602 MsG0180005338.01.T01:intron 20.0%
!! TAAACATGACTAATGTTTAA+TGG + Chr1:90518408-90518427 MsG0180005338.01.T01:intron 20.0%
!! TAAATCTTATCTATAATCTG+AGG + Chr1:90518664-90518683 MsG0180005338.01.T01:intron 20.0%
!! TAGAACAAGGTAATATAAAT+TGG - Chr1:90518746-90518765 None:intergenic 20.0%
!! TATAGTATGATGATTGAAAT+TGG - Chr1:90519255-90519274 None:intergenic 20.0%
!!! AAAGTTTTATTTGAGTTACA+AGG - Chr1:90518773-90518792 None:intergenic 20.0%
!!! ATAATTTTACTTATTGGCTT+TGG + Chr1:90518567-90518586 MsG0180005338.01.T01:CDS 20.0%
!!! CTTTGGATAATTTTACTTAT+TGG + Chr1:90518561-90518580 MsG0180005338.01.T01:CDS 20.0%
!!! CTTTTCTCCAAATATATTTA+TGG - Chr1:90519585-90519604 None:intergenic 20.0%
!!! TAATGTGATGTTTTCTATAT+AGG + Chr1:90519706-90519725 MsG0180005338.01.T01:intron 20.0%
!!! TGATTGATTTGCATAAAAAA+TGG + Chr1:90519022-90519041 MsG0180005338.01.T01:intron 20.0%
!!! TTCTTTTTTGGTTTAAATAC+AGG + Chr1:90518816-90518835 MsG0180005338.01.T01:intron 20.0%
! AAAGTAGTAAACCATGAAAT+AGG - Chr1:90519631-90519650 None:intergenic 25.0%
! AGTCATGTTTAAATGAAAAG+TGG - Chr1:90518399-90518418 None:intergenic 25.0%
! ATAAAAATACCACAGAGAAA+TGG - Chr1:90519383-90519402 None:intergenic 25.0%
! ATTATTACAAGAGAGACAAA+AGG - Chr1:90519158-90519177 None:intergenic 25.0%
! CAAAAATTGCACAACAATAT+AGG - Chr1:90519428-90519447 None:intergenic 25.0%
! GCAATAGAACATAATACATA+GGG - Chr1:90519480-90519499 None:intergenic 25.0%
! GCATAAAAAATGGTTAATCT+TGG + Chr1:90519032-90519051 MsG0180005338.01.T01:intron 25.0%
! GTCATGTTTAAATGAAAAGT+GGG - Chr1:90518398-90518417 None:intergenic 25.0%
! TATTTACAAGGAAGCAAATT+GGG + Chr1:90518527-90518546 MsG0180005338.01.T01:CDS 25.0%
! TCACATTATATTAGAACTGA+TGG - Chr1:90519694-90519713 None:intergenic 25.0%
! TTATTACAAGAGAGACAAAA+GGG - Chr1:90519157-90519176 None:intergenic 25.0%
! TTATTTACAAGGAAGCAAAT+TGG + Chr1:90518526-90518545 MsG0180005338.01.T01:CDS 25.0%
! TTTAAATATAGGTGCTCTAA+GGG + Chr1:90518886-90518905 MsG0180005338.01.T01:intron 25.0%
!! TCAATGTGAGCTTTTATAAT+AGG - Chr1:90519293-90519312 None:intergenic 25.0%
!! TTTTAAATATAGGTGCTCTA+AGG + Chr1:90518885-90518904 MsG0180005338.01.T01:intron 25.0%
!!! AAACTTTGAAACAAATGTCA+TGG + Chr1:90518786-90518805 MsG0180005338.01.T01:intron 25.0%
!!! ACAATAGAACACTTTGTTTT+TGG - Chr1:90519744-90519763 None:intergenic 25.0%
!!! TTTATTTTTCCATTTCTCTG+TGG + Chr1:90519371-90519390 MsG0180005338.01.T01:CDS 25.0%
AGAGAGTAGATTATGTAGTA+AGG + Chr1:90518124-90518143 MsG0180005338.01.T01:intron 30.0%
CAGATAGATATTGGGTATTA+TGG + Chr1:90518848-90518867 MsG0180005338.01.T01:intron 30.0%
CGCAATAGAACATAATACAT+AGG - Chr1:90519481-90519500 None:intergenic 30.0%
CTGAAGTACAGATAGATATT+GGG + Chr1:90518840-90518859 MsG0180005338.01.T01:intron 30.0%
! AAGAATCCTTTTCAGCATTT+TGG + Chr1:90519773-90519792 MsG0180005338.01.T01:CDS 30.0%
! AGAATCCTTTTCAGCATTTT+GGG + Chr1:90519774-90519793 MsG0180005338.01.T01:CDS 30.0%
! CATGGTTTCGTTTTCTTTTT+TGG + Chr1:90518804-90518823 MsG0180005338.01.T01:intron 30.0%
! CTCTTGTAATAATAGCTCTA+CGG + Chr1:90519165-90519184 MsG0180005338.01.T01:intron 30.0%
! TCTGAAGTACAGATAGATAT+TGG + Chr1:90518839-90518858 MsG0180005338.01.T01:intron 30.0%
! TGATGTTTTCTATATAGGAG+TGG + Chr1:90519711-90519730 MsG0180005338.01.T01:intron 30.0%
! TTTTTGTAGACATACTTGTG+TGG - Chr1:90519508-90519527 None:intergenic 30.0%
!!! TTTTACTTATTGGCTTTGGA+AGG + Chr1:90518571-90518590 MsG0180005338.01.T01:CDS 30.0%
AAGCTCACATTGAAGTTTCT+AGG + Chr1:90519300-90519319 MsG0180005338.01.T01:intron 35.0%
GTTACAAGGATAGTAGAACA+AGG - Chr1:90518759-90518778 None:intergenic 35.0%
! AGGCATCTCTTTTTAACAAG+AGG + Chr1:90518693-90518712 MsG0180005338.01.T01:intron 35.0%
! AGTTTTTGTAGATCTTCTCC+TGG - Chr1:90518191-90518210 None:intergenic 35.0%
! TCTATAATCTGAGGTATGAG+AGG + Chr1:90518673-90518692 MsG0180005338.01.T01:intron 35.0%
! TCTTGTCTAGGATCGTTTAA+AGG + Chr1:90519333-90519352 MsG0180005338.01.T01:intron 35.0%
AGAGCTGATGAAACTTCAGT+TGG - Chr1:90518470-90518489 None:intergenic 40.0%
AGTAAGGAGAGTAGATCAGA+TGG + Chr1:90518140-90518159 MsG0180005338.01.T01:intron 40.0%
CAACACCCAAAATGCTGAAA+AGG - Chr1:90519782-90519801 None:intergenic 40.0%
CCAATTTCCAAGTGGATACA+GGG + Chr1:90518940-90518959 MsG0180005338.01.T01:intron 40.0%
CTGACAGAAAGTCACATGTT+TGG + Chr1:90519073-90519092 MsG0180005338.01.T01:intron 40.0%
CTTATCCCACTATGTAGAGT+CGG - Chr1:90518290-90518309 None:intergenic 40.0%
GGCTGTATCTTATCTTGTCT+AGG + Chr1:90519321-90519340 MsG0180005338.01.T01:intron 40.0%
GTATACACTCTTGTCACTGA+TGG + Chr1:90519819-90519838 MsG0180005338.01.T01:CDS 40.0%
GTTCAGCAGAAGGAAATTGA+CGG - Chr1:90518376-90518395 None:intergenic 40.0%
TCCAATTTCCAAGTGGATAC+AGG + Chr1:90518939-90518958 MsG0180005338.01.T01:intron 40.0%
TGCTTCCTTGTAAATAACCC+AGG - Chr1:90518523-90518542 None:intergenic 40.0%
TGGAAAGAGATTCTGACACA+AGG - Chr1:90518450-90518469 None:intergenic 40.0%
! AGTGACAAGAGTGTATACCA+AGG - Chr1:90519817-90519836 None:intergenic 40.0%
! TATATTTGGAGAAAAGGCGC+AGG + Chr1:90519589-90519608 MsG0180005338.01.T01:intron 40.0%
! TGATGGAAAATTGGCTTAGG+TGG + Chr1:90518090-90518109 MsG0180005338.01.T01:CDS 40.0%
!! ATTTTGGGTGTTGTCATGCT+TGG + Chr1:90519789-90519808 MsG0180005338.01.T01:CDS 40.0%
AAGGAGAGTAGATCAGATGG+AGG + Chr1:90518143-90518162 MsG0180005338.01.T01:intron 45.0%
AGGAGAGTAGATCAGATGGA+GGG + Chr1:90518144-90518163 MsG0180005338.01.T01:intron 45.0%
ATCAGATCCCTGTATCCACT+TGG - Chr1:90518950-90518969 None:intergenic 45.0%
ATCCTGTCAGCTTGCACTAT+AGG - Chr1:90519229-90519248 None:intergenic 45.0%
ATTGGGTCAGCTCGATTCTT+TGG + Chr1:90518544-90518563 MsG0180005338.01.T01:CDS 45.0%
CCCTGTATCCACTTGGAAAT+TGG - Chr1:90518943-90518962 None:intergenic 45.0%
GACTCGTTCCAATTTCCAAG+TGG + Chr1:90518932-90518951 MsG0180005338.01.T01:intron 45.0%
GTCATCACAAGAGTCTTCAC+AGG + Chr1:90519453-90519472 MsG0180005338.01.T01:CDS 45.0%
TCAAATCATCAGAGAGAGCC+AGG + Chr1:90518170-90518189 MsG0180005338.01.T01:intron 45.0%
TGTTGTCATGCTTGGAACCT+TGG + Chr1:90519797-90519816 MsG0180005338.01.T01:CDS 45.0%
! ACGGAGTCAATAGCTCTGTA+CGG - Chr1:90519854-90519873 None:intergenic 45.0%
! ATGTAGCCGACTCTACATAG+TGG + Chr1:90518281-90518300 MsG0180005338.01.T01:intron 45.0%
! CGACTCTACATAGTGGGATA+AGG + Chr1:90518288-90518307 MsG0180005338.01.T01:intron 45.0%
! GTCAATAGCTCTGTACGGAA+AGG - Chr1:90519849-90519868 None:intergenic 45.0%
! GTTTTTCTGTCACACCAACC+AGG + Chr1:90518497-90518516 MsG0180005338.01.T01:intron 45.0%
! TGAAAAGTGGGTTCAGCAGA+AGG - Chr1:90518386-90518405 None:intergenic 45.0%
! TGTAGCCGACTCTACATAGT+GGG + Chr1:90518282-90518301 MsG0180005338.01.T01:intron 45.0%
CACCTATAGTGCAAGCTGAC+AGG + Chr1:90519224-90519243 MsG0180005338.01.T01:intron 50.0%
CCAGGCCTGGGTTATTTACA+AGG + Chr1:90518515-90518534 MsG0180005338.01.T01:intron 50.0%
CCTTGTAAATAACCCAGGCC+TGG - Chr1:90518518-90518537 None:intergenic 50.0%
CTATAGGTGCAACAGGTGCT+TGG - Chr1:90519213-90519232 None:intergenic 50.0%
GCTTGCACTATAGGTGCAAC+AGG - Chr1:90519220-90519239 None:intergenic 50.0%
GTAAATAACCCAGGCCTGGT+TGG - Chr1:90518514-90518533 None:intergenic 50.0%
CTGTCACACCAACCAGGCCT+GGG + Chr1:90518503-90518522 MsG0180005338.01.T01:intron 60.0%
TCTGTCACACCAACCAGGCC+TGG + Chr1:90518502-90518521 MsG0180005338.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 90518089 90519875 90518089 ID=MsG0180005338.01;Name=MsG0180005338.01
Chr1 mRNA 90518089 90519875 90518089 ID=MsG0180005338.01.T01;Parent=MsG0180005338.01;Name=MsG0180005338.01.T01;_AED=0.49;_eAED=0.62;_QI=0|0.33|0.25|1|0.33|0.25|4|0|147
Chr1 exon 90518089 90518140 90518089 ID=MsG0180005338.01.T01:exon:48017;Parent=MsG0180005338.01.T01
Chr1 exon 90518519 90518592 90518519 ID=MsG0180005338.01.T01:exon:48018;Parent=MsG0180005338.01.T01
Chr1 exon 90519343 90519512 90519343 ID=MsG0180005338.01.T01:exon:48019;Parent=MsG0180005338.01.T01
Chr1 exon 90519728 90519875 90519728 ID=MsG0180005338.01.T01:exon:48020;Parent=MsG0180005338.01.T01
Chr1 CDS 90518089 90518140 90518089 ID=MsG0180005338.01.T01:cds;Parent=MsG0180005338.01.T01
Chr1 CDS 90518519 90518592 90518519 ID=MsG0180005338.01.T01:cds;Parent=MsG0180005338.01.T01
Chr1 CDS 90519343 90519512 90519343 ID=MsG0180005338.01.T01:cds;Parent=MsG0180005338.01.T01
Chr1 CDS 90519728 90519875 90519728 ID=MsG0180005338.01.T01:cds;Parent=MsG0180005338.01.T01
Gene Sequence

>MsG0180005338.01.T01

ATGATGGAAAATTGGCTTAGGTGGTTTGAGCATGTAGAGAGTAGATTATGTAGCCTGGGTTATTTACAAGGAAGCAAATTGGGTCAGCTCGATTCTTTGGATAATTTTACTTATTGGCTTTGGAAGGATCGTTTAAAGGAATCTCACGTGCTGATTTATTTTTCCATTTCTCTGTGGTATTTTTATTGTTTCAGCATTGCTACATCTGCCTATATTGTTGTGCAATTTTTGAAGCTGTCATCACAAGAGTCTTCACAGGACCCTATGTATTATGTTCTATTGCGACATCCACACAAGAGTGGCACAGAACCAAAAACAAAGTGTTCTATTGTTGCGACTCTAAGAATCCTTTTCAGCATTTTGGGTGTTGTCATGCTTGGAACCTTGGTATACACTCTTGTCACTGATGGTTCTCCTTTCCGTACAGAGCTATTGACTCCGTAA

Protein sequence

>MsG0180005338.01.T01

MMENWLRWFEHVESRLCSLGYLQGSKLGQLDSLDNFTYWLWKDRLKESHVLIYFSISLWYFYCFSIATSAYIVVQFLKLSSQESSQDPMYYVLLRHPHKSGTEPKTKCSIVATLRILFSILGVVMLGTLVYTLVTDGSPFRTELLTP*