Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032512.01.T01 | XP_003620552.2 | 92.5 | 160 | 8 | 2 | 1 | 160 | 1 | 156 | 4.99E-99 | 292 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002891.01 | MsG0680032512.01 | 0.815724 | 7.981942e-52 | 5.546838e-49 |
| MsG0180005338.01 | MsG0680032512.01 | 0.842788 | 2.087836e-58 | 3.200162e-55 |
| MsG0180005898.01 | MsG0680032512.01 | 0.801415 | 9.101629e-49 | 4.344031e-46 |
| MsG0280011362.01 | MsG0680032512.01 | 0.817884 | 2.616137e-52 | 1.928619e-49 |
| MsG0380014144.01 | MsG0680032512.01 | 0.808636 | 2.810409e-50 | 1.615143e-47 |
| MsG0680032512.01 | MsG0680035623.01 | 0.881813 | 1.757359e-70 | 1.044374e-66 |
| MsG0680032512.01 | MsG0680035665.01 | 0.840903 | 6.569144e-58 | 9.493176e-55 |
| MsG0680032512.01 | MsG0780040154.01 | 0.805079 | 1.587563e-49 | 8.321876e-47 |
| MsG0680032512.01 | MsG0780040155.01 | 0.812633 | 3.844085e-51 | 2.456751e-48 |
| MsG0680032512.01 | MsG0780040175.01 | 0.813723 | 2.216000e-51 | 1.458380e-48 |
| MsG0680032512.01 | MsG0780041002.01 | 0.801243 | 9.872965e-49 | 4.691916e-46 |
| MsG0680032512.01 | MsG0780041129.01 | 0.817291 | 3.559445e-52 | 2.581641e-49 |
| MsG0680032512.01 | MsG0880044318.01 | 0.814210 | 1.730180e-51 | 1.153727e-48 |
| MsG0680032512.01 | MsG0880044453.01 | 0.844908 | 5.648473e-59 | 9.254655e-56 |
| MsG0680032512.01 | MsG0880045394.01 | 0.807583 | 4.709602e-50 | 2.632874e-47 |
| MsG0680032512.01 | MsG0880045761.01 | 0.827659 | 1.384346e-54 | 1.345006e-51 |
| MsG0480021892.01 | MsG0680032512.01 | 0.801462 | 8.901131e-49 | 4.253420e-46 |
| MsG0480022664.01 | MsG0680032512.01 | 0.818947 | 1.502459e-52 | 1.140703e-49 |
| MsG0480023386.01 | MsG0680032512.01 | 0.802365 | 5.808429e-49 | 2.840092e-46 |
| MsG0380016066.01 | MsG0680032512.01 | 0.822527 | 2.258152e-53 | 1.894980e-50 |
| MsG0380016151.01 | MsG0680032512.01 | 0.810031 | 1.410620e-50 | 8.413324e-48 |
| MsG0380016997.01 | MsG0680032512.01 | 0.821286 | 4.378137e-53 | 3.546685e-50 |
| MsG0380017353.01 | MsG0680032512.01 | 0.810704 | 1.009963e-50 | 6.131309e-48 |
| MsG0280006533.01 | MsG0680032512.01 | 0.804469 | 2.127942e-49 | 1.098030e-46 |
| MsG0280008139.01 | MsG0680032512.01 | 0.809293 | 2.032638e-50 | 1.188846e-47 |
| MsG0280010253.01 | MsG0680032512.01 | 0.805560 | 1.258715e-49 | 6.679911e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032512.01.T01 | MTR_6g086680 | 92.500 | 160 | 8 | 2 | 1 | 160 | 1 | 156 | 6.04e-103 | 292 |
| MsG0680032512.01.T01 | MTR_1g088260 | 35.185 | 108 | 67 | 2 | 46 | 153 | 3 | 107 | 2.83e-21 | 84.0 |
| MsG0680032512.01.T01 | MTR_1g088250 | 37.391 | 115 | 70 | 2 | 39 | 153 | 24 | 136 | 3.56e-21 | 84.7 |
| MsG0680032512.01.T01 | MTR_7g108775 | 31.944 | 144 | 85 | 3 | 10 | 153 | 12 | 142 | 2.14e-18 | 77.4 |
| MsG0680032512.01.T01 | MTR_7g108775 | 31.250 | 144 | 86 | 3 | 10 | 153 | 12 | 142 | 3.40e-18 | 77.8 |
| MsG0680032512.01.T01 | MTR_8g085580 | 31.304 | 115 | 71 | 3 | 43 | 154 | 34 | 143 | 4.95e-13 | 63.5 |
| MsG0680032512.01.T01 | MTR_8g085590 | 29.268 | 123 | 81 | 3 | 36 | 156 | 28 | 146 | 8.75e-12 | 60.1 |
| MsG0680032512.01.T01 | MTR_7g080280 | 37.069 | 116 | 64 | 5 | 42 | 153 | 44 | 154 | 1.09e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032512.01.T01 | AT5G19590 | 63.971 | 136 | 44 | 2 | 24 | 159 | 20 | 150 | 1.51e-58 | 179 |
| MsG0680032512.01.T01 | AT5G19590 | 63.971 | 136 | 44 | 2 | 24 | 159 | 20 | 150 | 1.51e-58 | 179 |
| MsG0680032512.01.T01 | AT1G02816 | 33.077 | 130 | 84 | 3 | 25 | 153 | 17 | 144 | 8.48e-21 | 84.0 |
| MsG0680032512.01.T01 | AT4G02370 | 33.043 | 115 | 75 | 2 | 39 | 153 | 32 | 144 | 1.32e-19 | 80.9 |
| MsG0680032512.01.T01 | AT1G02813 | 30.822 | 146 | 89 | 3 | 8 | 153 | 3 | 136 | 2.73e-19 | 79.7 |
| MsG0680032512.01.T01 | AT4G02360 | 33.913 | 115 | 73 | 2 | 39 | 153 | 29 | 140 | 1.04e-16 | 73.2 |
| MsG0680032512.01.T01 | AT5G19860 | 31.013 | 158 | 94 | 5 | 1 | 154 | 3 | 149 | 4.51e-14 | 66.6 |
Find 50 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGAACACGATGAGAAATTT+TGG | 0.186460 | 6:-43490741 | None:intergenic |
| TTGCGGAGCTTGATGGAATT+AGG | 0.228054 | 6:+43491045 | MsG0680032512.01.T01:CDS |
| TTTAAGTGGTGGGGTATAAC+TGG | 0.257953 | 6:+43491080 | MsG0680032512.01.T01:CDS |
| GATGGGGATGGAATTGATTT+GGG | 0.296449 | 6:-43490822 | None:intergenic |
| TAATTACGTTGCCACGTATT+CGG | 0.311769 | 6:+43490989 | MsG0680032512.01.T01:CDS |
| ATGATGTTGTGTTTGAAGTT+GGG | 0.341877 | 6:+43491120 | MsG0680032512.01.T01:CDS |
| GGATGGGGATGGAATTGATT+TGG | 0.343332 | 6:-43490823 | None:intergenic |
| TGCGGAGCTTGATGGAATTA+GGG | 0.345909 | 6:+43491046 | MsG0680032512.01.T01:CDS |
| TGGTTTAAGACGGTTGTTGC+AGG | 0.360772 | 6:-43490894 | None:intergenic |
| GATGATGTTGTGTTTGAAGT+TGG | 0.363664 | 6:+43491119 | MsG0680032512.01.T01:CDS |
| ATAACTGGGATTAGGTCTTC+TGG | 0.369879 | 6:+43491095 | MsG0680032512.01.T01:CDS |
| GTTCTATGTGTGCTGGGGAT+GGG | 0.377852 | 6:-43490839 | None:intergenic |
| TTCTATGTGTGCTGGGGATG+GGG | 0.389644 | 6:-43490838 | None:intergenic |
| GGGAAAATTGCGGAGCTTGA+TGG | 0.391220 | 6:+43491038 | MsG0680032512.01.T01:CDS |
| GTTGTGAGTTCTATGTGTGC+TGG | 0.403207 | 6:-43490846 | None:intergenic |
| AGTTCTATGTGTGCTGGGGA+TGG | 0.408651 | 6:-43490840 | None:intergenic |
| ACGTATTCGGATACAATTAC+AGG | 0.413235 | 6:+43491002 | MsG0680032512.01.T01:CDS |
| ATGTGTGCTGGGGATGGGGA+TGG | 0.446415 | 6:-43490834 | None:intergenic |
| GGTTGTTACGGCGAAGTATC+CGG | 0.473474 | 6:+43491142 | MsG0680032512.01.T01:CDS |
| ATGGGGATGGAATTGATTTG+GGG | 0.485101 | 6:-43490821 | None:intergenic |
| TTGTGAGTTCTATGTGTGCT+GGG | 0.488652 | 6:-43490845 | None:intergenic |
| TGATGTTGTGTTTGAAGTTG+GGG | 0.490462 | 6:+43491121 | MsG0680032512.01.T01:CDS |
| ACACGCGCCGGCGAATTTGA+CGG | 0.494053 | 6:-43490946 | None:intergenic |
| GTTTGAAGTTGGGGTTGTTA+CGG | 0.494090 | 6:+43491130 | MsG0680032512.01.T01:CDS |
| ACAACCTACGGTTTCCCAAT+CGG | 0.501786 | 6:+43490864 | MsG0680032512.01.T01:CDS |
| TTAAGTGGTGGGGTATAACT+GGG | 0.506025 | 6:+43491081 | MsG0680032512.01.T01:CDS |
| GAATGCTTTCCTTCACAAGC+AGG | 0.513403 | 6:-43491185 | None:intergenic |
| GGTGGGGTATAACTGGGATT+AGG | 0.514349 | 6:+43491087 | MsG0680032512.01.T01:CDS |
| CACTTCATCAACAAAACCTC+CGG | 0.519364 | 6:+43490915 | MsG0680032512.01.T01:CDS |
| TACGTGGCAACGTAATTATC+CGG | 0.533926 | 6:-43490984 | None:intergenic |
| TTGCAGGGAGAAGACCGATT+GGG | 0.540559 | 6:-43490878 | None:intergenic |
| GTGTAAAATCACGCTCCCAC+CGG | 0.559202 | 6:+43490965 | MsG0680032512.01.T01:CDS |
| ATTACAGGGAAGATTGTGAA+AGG | 0.563753 | 6:+43491017 | MsG0680032512.01.T01:CDS |
| CACATAGAACTCACAACCTA+CGG | 0.571226 | 6:+43490852 | MsG0680032512.01.T01:CDS |
| CTATCAACAAAATTCTTCAC+CGG | 0.573314 | 6:-43491161 | None:intergenic |
| GGTTTAAGACGGTTGTTGCA+GGG | 0.573978 | 6:-43490893 | None:intergenic |
| GAATTTGACGGAGAATTCGC+CGG | 0.577056 | 6:-43490934 | None:intergenic |
| GTTGATGAAGTGGTTTAAGA+CGG | 0.583505 | 6:-43490904 | None:intergenic |
| GAATTCTCCGTCAAATTCGC+CGG | 0.588379 | 6:+43490939 | MsG0680032512.01.T01:CDS |
| TTACAGGGAAGATTGTGAAA+GGG | 0.595921 | 6:+43491018 | MsG0680032512.01.T01:CDS |
| GTTGCAGGGAGAAGACCGAT+TGG | 0.601695 | 6:-43490879 | None:intergenic |
| CGTATTCGGATACAATTACA+GGG | 0.611663 | 6:+43491003 | MsG0680032512.01.T01:CDS |
| GTTGATAGTCCTGCTTGTGA+AGG | 0.624809 | 6:+43491176 | MsG0680032512.01.T01:CDS |
| GTGGCAACGTAATTATCCGG+TGG | 0.647540 | 6:-43490981 | None:intergenic |
| TGTGAGTTCTATGTGTGCTG+GGG | 0.672195 | 6:-43490844 | None:intergenic |
| TGGCAACGTAATTATCCGGT+GGG | 0.675831 | 6:-43490980 | None:intergenic |
| TGTAATTGTATCCGAATACG+TGG | 0.697904 | 6:-43491000 | None:intergenic |
| AAGACCGATTGGGAAACCGT+AGG | 0.704857 | 6:-43490868 | None:intergenic |
| TTTGACGGAGAATTCGCCGG+AGG | 0.706889 | 6:-43490931 | None:intergenic |
| GATTGTGAAAGGGAAAATTG+CGG | 0.708863 | 6:+43491028 | MsG0680032512.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTATCAACAAAATTCTTCAC+CGG | - | Chr6:43491164-43491183 | None:intergenic | 30.0% | |
| ! | ATGATGTTGTGTTTGAAGTT+GGG | + | Chr6:43491120-43491139 | MsG0680032512.01.T01:CDS | 30.0% |
| !!! | GAATTGATTTGGGGATTTTA+GGG | - | Chr6:43490815-43490834 | None:intergenic | 30.0% |
| ACGTATTCGGATACAATTAC+AGG | + | Chr6:43491002-43491021 | MsG0680032512.01.T01:CDS | 35.0% | |
| ATTACAGGGAAGATTGTGAA+AGG | + | Chr6:43491017-43491036 | MsG0680032512.01.T01:CDS | 35.0% | |
| CGTATTCGGATACAATTACA+GGG | + | Chr6:43491003-43491022 | MsG0680032512.01.T01:CDS | 35.0% | |
| GATTGTGAAAGGGAAAATTG+CGG | + | Chr6:43491028-43491047 | MsG0680032512.01.T01:CDS | 35.0% | |
| TAATTACGTTGCCACGTATT+CGG | + | Chr6:43490989-43491008 | MsG0680032512.01.T01:CDS | 35.0% | |
| TGTAATTGTATCCGAATACG+TGG | - | Chr6:43491003-43491022 | None:intergenic | 35.0% | |
| TTACAGGGAAGATTGTGAAA+GGG | + | Chr6:43491018-43491037 | MsG0680032512.01.T01:CDS | 35.0% | |
| ! | ACGAACACGATGAGAAATTT+TGG | - | Chr6:43490744-43490763 | None:intergenic | 35.0% |
| ! | GATGATGTTGTGTTTGAAGT+TGG | + | Chr6:43491119-43491138 | MsG0680032512.01.T01:CDS | 35.0% |
| ! | GTTGATGAAGTGGTTTAAGA+CGG | - | Chr6:43490907-43490926 | None:intergenic | 35.0% |
| ! | TGATGTTGTGTTTGAAGTTG+GGG | + | Chr6:43491121-43491140 | MsG0680032512.01.T01:CDS | 35.0% |
| !!! | ATTTGGGGATTTTAGGGTTA+GGG | - | Chr6:43490809-43490828 | None:intergenic | 35.0% |
| !!! | GGAATTGATTTGGGGATTTT+AGG | - | Chr6:43490816-43490835 | None:intergenic | 35.0% |
| ATAACTGGGATTAGGTCTTC+TGG | + | Chr6:43491095-43491114 | MsG0680032512.01.T01:CDS | 40.0% | |
| CACATAGAACTCACAACCTA+CGG | + | Chr6:43490852-43490871 | MsG0680032512.01.T01:CDS | 40.0% | |
| CACTTCATCAACAAAACCTC+CGG | + | Chr6:43490915-43490934 | MsG0680032512.01.T01:CDS | 40.0% | |
| TACGTGGCAACGTAATTATC+CGG | - | Chr6:43490987-43491006 | None:intergenic | 40.0% | |
| TTGTGAGTTCTATGTGTGCT+GGG | - | Chr6:43490848-43490867 | None:intergenic | 40.0% | |
| ! | GATGGGGATGGAATTGATTT+GGG | - | Chr6:43490825-43490844 | None:intergenic | 40.0% |
| ! | GTTTGAAGTTGGGGTTGTTA+CGG | + | Chr6:43491130-43491149 | MsG0680032512.01.T01:CDS | 40.0% |
| ! | TTAAGTGGTGGGGTATAACT+GGG | + | Chr6:43491081-43491100 | MsG0680032512.01.T01:CDS | 40.0% |
| ! | TTTAAGTGGTGGGGTATAAC+TGG | + | Chr6:43491080-43491099 | MsG0680032512.01.T01:CDS | 40.0% |
| !! | ATGGGGATGGAATTGATTTG+GGG | - | Chr6:43490824-43490843 | None:intergenic | 40.0% |
| !!! | GATTTGGGGATTTTAGGGTT+AGG | - | Chr6:43490810-43490829 | None:intergenic | 40.0% |
| !!! | GGATTTTAGGGTTAGGGTTA+GGG | - | Chr6:43490803-43490822 | None:intergenic | 40.0% |
| !!! | TCGTGCGTTTTTTAAGTGGT+GGG | + | Chr6:43491070-43491089 | MsG0680032512.01.T01:CDS | 40.0% |
| !!! | TTCGTGCGTTTTTTAAGTGG+TGG | + | Chr6:43491069-43491088 | MsG0680032512.01.T01:CDS | 40.0% |
| ACAACCTACGGTTTCCCAAT+CGG | + | Chr6:43490864-43490883 | MsG0680032512.01.T01:CDS | 45.0% | |
| GAATGCTTTCCTTCACAAGC+AGG | - | Chr6:43491188-43491207 | None:intergenic | 45.0% | |
| GAATTCTCCGTCAAATTCGC+CGG | + | Chr6:43490939-43490958 | MsG0680032512.01.T01:CDS | 45.0% | |
| GAATTTGACGGAGAATTCGC+CGG | - | Chr6:43490937-43490956 | None:intergenic | 45.0% | |
| GGATGGGGATGGAATTGATT+TGG | - | Chr6:43490826-43490845 | None:intergenic | 45.0% | |
| GTTGTGAGTTCTATGTGTGC+TGG | - | Chr6:43490849-43490868 | None:intergenic | 45.0% | |
| TGCGGAGCTTGATGGAATTA+GGG | + | Chr6:43491046-43491065 | MsG0680032512.01.T01:CDS | 45.0% | |
| TGGCAACGTAATTATCCGGT+GGG | - | Chr6:43490983-43491002 | None:intergenic | 45.0% | |
| TGTGAGTTCTATGTGTGCTG+GGG | - | Chr6:43490847-43490866 | None:intergenic | 45.0% | |
| TTGCGGAGCTTGATGGAATT+AGG | + | Chr6:43491045-43491064 | MsG0680032512.01.T01:CDS | 45.0% | |
| ! | GGTTTAAGACGGTTGTTGCA+GGG | - | Chr6:43490896-43490915 | None:intergenic | 45.0% |
| ! | TGGTTTAAGACGGTTGTTGC+AGG | - | Chr6:43490897-43490916 | None:intergenic | 45.0% |
| !! | GTTGATAGTCCTGCTTGTGA+AGG | + | Chr6:43491176-43491195 | MsG0680032512.01.T01:CDS | 45.0% |
| !!! | CGGAGGTTTTGTTGATGAAG+TGG | - | Chr6:43490917-43490936 | None:intergenic | 45.0% |
| !!! | CGTGCGTTTTTTAAGTGGTG+GGG | + | Chr6:43491071-43491090 | MsG0680032512.01.T01:CDS | 45.0% |
| !!! | GGGATTTTAGGGTTAGGGTT+AGG | - | Chr6:43490804-43490823 | None:intergenic | 45.0% |
| !!! | GGGTTCGTGCGTTTTTTAAG+TGG | + | Chr6:43491066-43491085 | MsG0680032512.01.T01:CDS | 45.0% |
| AAGACCGATTGGGAAACCGT+AGG | - | Chr6:43490871-43490890 | None:intergenic | 50.0% | |
| AGTTCTATGTGTGCTGGGGA+TGG | - | Chr6:43490843-43490862 | None:intergenic | 50.0% | |
| GGGAAAATTGCGGAGCTTGA+TGG | + | Chr6:43491038-43491057 | MsG0680032512.01.T01:CDS | 50.0% | |
| GGTGGGGTATAACTGGGATT+AGG | + | Chr6:43491087-43491106 | MsG0680032512.01.T01:CDS | 50.0% | |
| GTGGCAACGTAATTATCCGG+TGG | - | Chr6:43490984-43491003 | None:intergenic | 50.0% | |
| GTGTAAAATCACGCTCCCAC+CGG | + | Chr6:43490965-43490984 | MsG0680032512.01.T01:CDS | 50.0% | |
| GTTCTATGTGTGCTGGGGAT+GGG | - | Chr6:43490842-43490861 | None:intergenic | 50.0% | |
| TTCTATGTGTGCTGGGGATG+GGG | - | Chr6:43490841-43490860 | None:intergenic | 50.0% | |
| TTGCAGGGAGAAGACCGATT+GGG | - | Chr6:43490881-43490900 | None:intergenic | 50.0% | |
| ! | GGTTGTTACGGCGAAGTATC+CGG | + | Chr6:43491142-43491161 | MsG0680032512.01.T01:CDS | 50.0% |
| GTTGCAGGGAGAAGACCGAT+TGG | - | Chr6:43490882-43490901 | None:intergenic | 55.0% | |
| TTTGACGGAGAATTCGCCGG+AGG | - | Chr6:43490934-43490953 | None:intergenic | 55.0% | |
| ! | GAGCGTGATTTTACACGCGC+CGG | - | Chr6:43490961-43490980 | None:intergenic | 55.0% |
| ATGTGTGCTGGGGATGGGGA+TGG | - | Chr6:43490837-43490856 | None:intergenic | 60.0% | |
| ! | ACACGCGCCGGCGAATTTGA+CGG | - | Chr6:43490949-43490968 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 43490735 | 43491217 | 43490735 | ID=MsG0680032512.01;Name=MsG0680032512.01 |
| Chr6 | mRNA | 43490735 | 43491217 | 43490735 | ID=MsG0680032512.01.T01;Parent=MsG0680032512.01;Name=MsG0680032512.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|160 |
| Chr6 | exon | 43490735 | 43491217 | 43490735 | ID=MsG0680032512.01.T01:exon:21598;Parent=MsG0680032512.01.T01 |
| Chr6 | CDS | 43490735 | 43491217 | 43490735 | ID=MsG0680032512.01.T01:cds;Parent=MsG0680032512.01.T01 |
| Gene Sequence |
| Protein sequence |
