Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036585.01.T01 | XP_003621492.1 | 83.562 | 73 | 12 | 0 | 1 | 73 | 378 | 450 | 1.35E-35 | 137 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047885.01 | MsG0780036585.01 | 0.820437 | 6.862000e-53 | 5.429530e-50 |
| MsG0080048065.01 | MsG0780036585.01 | 0.809128 | 2.204689e-50 | 1.283832e-47 |
| MsG0080048660.01 | MsG0780036585.01 | 0.812559 | 3.988284e-51 | 2.543962e-48 |
| MsG0080048727.01 | MsG0780036585.01 | 0.833186 | 6.161730e-56 | 7.042984e-53 |
| MsG0080048748.01 | MsG0780036585.01 | 0.845827 | 3.184649e-59 | 5.374919e-56 |
| MsG0080048773.01 | MsG0780036585.01 | 0.857196 | 1.923882e-62 | 4.709941e-59 |
| MsG0080048802.01 | MsG0780036585.01 | 0.811291 | 7.536322e-51 | 4.646720e-48 |
| MsG0080048845.01 | MsG0780036585.01 | 0.816349 | 5.790056e-52 | 4.092946e-49 |
| MsG0080048849.01 | MsG0780036585.01 | 0.859060 | 5.370400e-63 | 1.399800e-59 |
| MsG0080048878.01 | MsG0780036585.01 | 0.828496 | 8.705583e-55 | 8.667913e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036585.01.T01 | MTR_7g014610 | 83.562 | 73 | 12 | 0 | 1 | 73 | 378 | 450 | 1.64e-39 | 137 |
| MsG0780036585.01.T01 | MTR_7g014620 | 89.041 | 73 | 8 | 0 | 1 | 73 | 335 | 407 | 4.02e-32 | 117 |
| MsG0780036585.01.T01 | MTR_7g014670 | 69.014 | 71 | 21 | 1 | 1 | 71 | 163 | 232 | 2.44e-27 | 101 |
| MsG0780036585.01.T01 | MTR_7g014200 | 66.197 | 71 | 24 | 0 | 1 | 71 | 371 | 441 | 4.70e-27 | 103 |
| MsG0780036585.01.T01 | MTR_7g014160 | 64.789 | 71 | 24 | 1 | 1 | 71 | 377 | 446 | 3.07e-25 | 99.0 |
| MsG0780036585.01.T01 | MTR_7g013850 | 57.353 | 68 | 28 | 1 | 4 | 71 | 387 | 453 | 4.21e-18 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036585.01.T01 | AT5G39080 | 53.571 | 56 | 26 | 0 | 32 | 87 | 395 | 450 | 9.36e-13 | 63.9 |
| MsG0780036585.01.T01 | AT5G39050 | 44.776 | 67 | 37 | 0 | 21 | 87 | 390 | 456 | 2.65e-12 | 62.8 |
| MsG0780036585.01.T01 | AT3G29670 | 49.153 | 59 | 29 | 1 | 30 | 87 | 380 | 438 | 2.89e-12 | 62.4 |
Find 30 sgRNAs with CRISPR-Local
Find 139 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAGAGATGTCTCATGCGC+AGG | 0.465689 | 7:+10099471 | MsG0780036585.01.T01:CDS |
| AGAGATGTCTCATGCACATG+GGG | 0.633067 | 7:+10099323 | MsG0780036585.01.T01:CDS |
| ATAAAGATGATAAATGATAA+TGG | 0.401064 | 7:+10097431 | None:intergenic |
| ATATAATTCTTTGATAAATG+AGG | 0.421850 | 7:+10097490 | MsG0780036585.01.T01:CDS |
| ATGACACACAGAGCCACACG+AGG | 0.723770 | 7:+10099443 | MsG0780036585.01.T01:CDS |
| CAGAGATGTCTCATGCACAT+GGG | 0.624189 | 7:+10099322 | MsG0780036585.01.T01:CDS |
| CAGAGATGTCTCATGCGCAG+GGG | 0.668606 | 7:+10099473 | MsG0780036585.01.T01:CDS |
| CCAGAGATGTCTCATGCACA+TGG | 0.556944 | 7:+10099321 | MsG0780036585.01.T01:CDS |
| CCAGAGATGTCTCATGCGCA+GGG | 0.526491 | 7:+10099472 | MsG0780036585.01.T01:CDS |
| CCATGTGCATGAGACATCTC+TGG | 0.540821 | 7:-10099321 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACTAAACCTCTCTTGATTA+GGG | - | Chr7:10099004-10099023 | None:intergenic | 30.0% | |
| ATACTCTCTTAAATAAAGGC+AGG | - | Chr7:10098678-10098697 | None:intergenic | 30.0% | |
| GTAGATAGAGGTTTAATCAT+TGG | + | Chr7:10097608-10097627 | MsG0780036585.01.T01:CDS | 30.0% | |
| TAACTAAACCTCTCTTGATT+AGG | - | Chr7:10099005-10099024 | None:intergenic | 30.0% | |
| TCTTTGATAAATGAGGGATT+TGG | + | Chr7:10097497-10097516 | MsG0780036585.01.T01:CDS | 30.0% | |
| TTATTCATTCAGAAAGTCAG+CGG | - | Chr7:10099139-10099158 | None:intergenic | 30.0% | |
| TTGGAAACATTAGAGTTACA+TGG | + | Chr7:10098104-10098123 | MsG0780036585.01.T01:intron | 30.0% | |
| AAGAGAGGTTTAGTTACACA+TGG | + | Chr7:10099009-10099028 | MsG0780036585.01.T01:intron | 35.0% | |
| AAGGACTTTGCTTAATTGAC+AGG | + | Chr7:10097723-10097742 | MsG0780036585.01.T01:intron | 35.0% | |
| AAGTTGAACGACAATTCCTA+CGG | - | Chr7:10098478-10098497 | None:intergenic | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 10097437 | 10099510 | 10097437 | ID=MsG0780036585.01;Name=MsG0780036585.01 |
| Chr7 | mRNA | 10097437 | 10099510 | 10097437 | ID=MsG0780036585.01.T01;Parent=MsG0780036585.01;Name=MsG0780036585.01.T01;_AED=0.68;_eAED=0.68;_QI=0|0|0|0.33|1|1|3|0|125 |
| Chr7 | exon | 10097437 | 10097656 | 10097437 | ID=MsG0780036585.01.T01:exon:10374;Parent=MsG0780036585.01.T01 |
| Chr7 | exon | 10099289 | 10099367 | 10099289 | ID=MsG0780036585.01.T01:exon:10375;Parent=MsG0780036585.01.T01 |
| Chr7 | exon | 10099432 | 10099510 | 10099432 | ID=MsG0780036585.01.T01:exon:10376;Parent=MsG0780036585.01.T01 |
| Chr7 | CDS | 10097437 | 10097656 | 10097437 | ID=MsG0780036585.01.T01:cds;Parent=MsG0780036585.01.T01 |
| Chr7 | CDS | 10099289 | 10099367 | 10099289 | ID=MsG0780036585.01.T01:cds;Parent=MsG0780036585.01.T01 |
| Chr7 | CDS | 10099432 | 10099510 | 10099432 | ID=MsG0780036585.01.T01:cds;Parent=MsG0780036585.01.T01 |
| Gene Sequence |
>MsG0780036585.01.T01 ATGATAAATGATAATGGTGTTTTTCAAGGATCTATTAATGATGGGTTTGACAAATATAATTCTTTGATAAATGAGGGATTTGGTGTTTTTGGAGTTGGGGGGTCTAATAAATTTGGTGTTTATGAGACTGATTTTGGTTGGGGAAGGCCAAAAAAGGTGGAGATGGTGTCGGTAGATAGAGGTTTAATCATTGGTTTGGCAGAGAGCAAAGATGAAAATGATGCGCACATGATGACAAACACGAGGAAGCCACCAGAGATGTCTCATGCACATGGGGCGCATGGGGCAGTAGCCACAGTATGCGCACATGATGACACACAGAGCCACACGAGGAAGCCACCAGAGATGTCTCATGCGCAGGGGCGCACGGGGCAGTAG |
| Protein sequence |
>MsG0780036585.01.T01 MINDNGVFQGSINDGFDKYNSLINEGFGVFGVGGSNKFGVYETDFGWGRPKKVEMVSVDRGLIIGLAESKDENDAHMMTNTRKPPEMSHAHGAHGAVATVCAHDDTQSHTRKPPEMSHAQGRTGQ* |
