Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037869.01.T01 | YP_010173446.1 | 91.489 | 47 | 4 | 0 | 1 | 47 | 35 | 81 | 1.62E-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037869.01.T01 | A4QLC8 | 84.783 | 46 | 7 | 0 | 1 | 46 | 33 | 78 | 9.40E-19 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037869.01.T01 | A0A898NCA2 | 91.489 | 47 | 4 | 0 | 1 | 47 | 35 | 81 | 7.74e-19 | 82.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013922.01 | MsG0780037869.01 | 0.856940 | 2.289644e-62 | 5.556237e-59 |
| MsG0580024908.01 | MsG0780037869.01 | 0.819069 | 1.409295e-52 | 1.073458e-49 |
| MsG0780037769.01 | MsG0780037869.01 | 0.828740 | 7.597662e-55 | 7.617827e-52 |
| MsG0780037869.01 | MsG0780038139.01 | 0.853479 | 2.326267e-61 | 5.033196e-58 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037869.01.T01 | MTR_4g051350 | 86.957 | 46 | 6 | 0 | 1 | 46 | 118 | 163 | 7.81e-20 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037869.01.T01 | ATCG00650 | 82.609 | 46 | 8 | 0 | 1 | 46 | 33 | 78 | 8.82e-19 | 72.8 |
Find 3 sgRNAs with CRISPR-Local
Find 4 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAAGATAAAATACGAGCT+TGG | 0.526187 | 7:+35098661 | None:intergenic |
| TATCGACTTATTAGTCAACA+TGG | 0.624658 | 7:-35098753 | MsG0780037869.01.T01:CDS |
| AAAAGGTGACGAATTAACAA+AGG | 0.651228 | 7:+35098636 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTAATCGTTGTTGTTTTA+AGG | + | Chr7:35098702-35098721 | None:intergenic | 20.0% |
| AAAAGGTGACGAATTAACAA+AGG | + | Chr7:35098764-35098783 | None:intergenic | 30.0% | |
| ACAAAGATAAAATACGAGCT+TGG | + | Chr7:35098739-35098758 | None:intergenic | 30.0% | |
| !! | TATCGACTTATTAGTCAACA+TGG | - | Chr7:35098644-35098663 | MsG0780037869.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 35098632 | 35098787 | 35098632 | ID=MsG0780037869.01;Name=MsG0780037869.01 |
| Chr7 | mRNA | 35098632 | 35098787 | 35098632 | ID=MsG0780037869.01.T01;Parent=MsG0780037869.01;Name=MsG0780037869.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|51 |
| Chr7 | exon | 35098632 | 35098787 | 35098632 | ID=MsG0780037869.01.T01:exon:16129;Parent=MsG0780037869.01.T01 |
| Chr7 | CDS | 35098632 | 35098787 | 35098632 | ID=MsG0780037869.01.T01:cds;Parent=MsG0780037869.01.T01 |
| Chr7 | mRNA | 35098730 | 35098756 | 35098730 | ID=MsG0780037869.01.T02;Parent=MsG0780037869.01;Name=MsG0780037869.01.T02;_AED=0.48;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|8 |
| Chr7 | exon | 35098730 | 35098756 | 35098730 | ID=MsG0780037869.01.T02:exon:16130;Parent=MsG0780037869.01.T02 |
| Chr7 | CDS | 35098730 | 35098756 | 35098730 | ID=MsG0780037869.01.T02:cds;Parent=MsG0780037869.01.T02 |
| Gene Sequence |
| Protein sequence |
