AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039117.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039117.01.T01 MTR_0474s0020 64.130 184 59 2 35 211 85 268 1.17e-45 154
MsG0780039117.01.T01 MTR_0474s0020 69.444 144 42 1 35 176 85 228 2.86e-44 149
MsG0780039117.01.T01 MTR_7g066640 78.095 105 23 0 7 111 95 199 1.99e-41 140
MsG0780039117.01.T01 MTR_4g015930 40.141 142 84 1 65 206 131 271 1.11e-18 85.1
MsG0780039117.01.T01 MTR_4g015930 33.971 209 116 9 6 207 129 322 5.88e-12 65.1
MsG0780039117.01.T01 MTR_4g015930 33.333 201 124 6 6 206 178 368 4.52e-11 62.8
MsG0780039117.01.T01 MTR_4g015930 40.141 142 84 1 65 206 131 271 1.14e-18 84.7
MsG0780039117.01.T01 MTR_4g015930 33.971 209 116 9 6 207 129 322 5.59e-12 65.1
MsG0780039117.01.T01 MTR_4g015930 33.333 201 124 6 6 206 178 368 4.18e-11 62.8
MsG0780039117.01.T01 MTR_4g015930 40.141 142 84 1 65 206 131 271 1.27e-18 84.7
MsG0780039117.01.T01 MTR_4g015930 33.971 209 116 9 6 207 129 322 6.81e-12 65.1
MsG0780039117.01.T01 MTR_4g015930 33.333 201 124 6 6 206 178 368 5.43e-11 62.4
MsG0780039117.01.T01 MTR_7g066600 71.642 67 19 0 7 73 118 184 1.05e-17 78.6
MsG0780039117.01.T01 MTR_7g066620 64.865 74 23 1 142 212 20 93 4.28e-16 77.4
MsG0780039117.01.T01 MTR_0017s0270 34.270 178 95 4 52 208 151 327 7.06e-14 70.1
MsG0780039117.01.T01 MTR_0087s0050 33.810 210 130 5 9 212 149 355 7.48e-14 70.9
MsG0780039117.01.T01 MTR_0061s0050 32.273 220 119 5 10 208 700 910 1.32e-12 67.0
MsG0780039117.01.T01 MTR_0017s0230 32.273 220 119 5 10 208 665 875 1.69e-12 66.6
MsG0780039117.01.T01 MTR_2g064580 36.145 166 82 4 66 208 309 473 2.16e-12 66.2
MsG0780039117.01.T01 MTR_2g069880 36.145 166 82 4 66 208 296 460 2.24e-12 66.2
MsG0780039117.01.T01 MTR_5g069580 32.314 229 111 5 10 208 589 803 3.52e-12 65.9
MsG0780039117.01.T01 MTR_6g037750 33.529 170 83 4 66 210 941 1105 9.25e-12 64.7
MsG0780039117.01.T01 MTR_1g047190 34.884 172 77 4 66 208 222 387 1.65e-11 63.5
MsG0780039117.01.T01 MTR_4g016870 44.681 94 40 3 116 203 132 219 2.85e-11 62.0
MsG0780039117.01.T01 MTR_7g039330 36.620 142 87 2 65 206 422 560 3.79e-11 62.8
MsG0780039117.01.T01 MTR_4g016850 38.760 129 62 3 87 206 100 220 3.81e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780039117.01.T01 AT1G58190 29.703 202 128 5 7 206 173 362 7.34e-12 65.1
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.43e-11 63.9
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.71e-11 63.9
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.71e-11 63.9
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.71e-11 63.9
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.81e-11 63.5
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.81e-11 63.5
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.81e-11 63.5
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.81e-11 63.5
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.81e-11 63.5
MsG0780039117.01.T01 AT4G39270 33.721 172 111 2 35 206 94 262 1.93e-11 63.5
MsG0780039117.01.T01 AT4G08850 34.752 141 89 2 66 206 501 638 3.73e-11 62.8
MsG0780039117.01.T01 AT4G08850 34.752 141 89 2 66 206 501 638 4.00e-11 62.8

Find 45 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGACGTGGAAGACCATTT+AGG 0.244814 7:+58199012 None:intergenic
TTATGCAGGGTTGAATAATT+TGG 0.246685 7:-58199909 MsG0780039117.01.T01:intron
AATATTATTTCATCCCTAAA+TGG 0.301362 7:-58199026 MsG0780039117.01.T01:CDS
TATATCAAAACTAAGGTCTT+TGG 0.310877 7:-58199084 MsG0780039117.01.T01:CDS
GAGACGTGGAAGACCATTTA+GGG 0.325733 7:+58199013 None:intergenic
ATCTTTGAAGGAACTCCATT+TGG 0.330502 7:-58199521 MsG0780039117.01.T01:CDS
TATCAGCATTCTGTCTTGTT+TGG 0.333685 7:-58199849 MsG0780039117.01.T01:CDS
TTCAATGAAGTTGCTACATC+TGG 0.345421 7:-58199608 MsG0780039117.01.T01:CDS
ATGCAAGGACAGTAATATCT+AGG 0.392346 7:+58198952 None:intergenic
CCATTTGGATTGGATTGGAT+TGG 0.399969 7:-58199506 MsG0780039117.01.T01:CDS
AGCATTCTGTCTTGTTTGGA+TGG 0.421623 7:-58199845 MsG0780039117.01.T01:CDS
GAACTCCATTTGGATTGGAT+TGG 0.424282 7:-58199511 MsG0780039117.01.T01:CDS
AGTGTCTTTGAAGTTGTTGT+TGG 0.427478 7:+58199298 None:intergenic
TACTCTATGACTTCATTTCA+CGG 0.437441 7:-58199785 MsG0780039117.01.T01:intron
GATATTGCATCAGCTCTTAG+TGG 0.442598 7:-58199275 MsG0780039117.01.T01:CDS
TTGTAAATTCAAACTCTTGA+GGG 0.444301 7:+58199244 None:intergenic
TGAAGGAACTCCATTTGGAT+TGG 0.452301 7:-58199516 MsG0780039117.01.T01:CDS
CAGCGGATTCACATCTTTGA+AGG 0.465120 7:-58199533 MsG0780039117.01.T01:CDS
CATAGAGTACATAGCAGGTA+AGG 0.479184 7:+58199799 None:intergenic
CCAATCCAATCCAATCCAAA+TGG 0.496880 7:+58199506 None:intergenic
ATTGAATGCAACACCACCAC+TGG 0.497018 7:-58200015 None:intergenic
TAATTTGGAAGTTCTTCACT+TGG 0.500946 7:-58199894 MsG0780039117.01.T01:CDS
TCAAACTCTTGAGGGAAGAA+AGG 0.501122 7:+58199252 None:intergenic
TAGCTTCGCCGCTGCATGCA+AGG 0.509883 7:+58198937 None:intergenic
GGATTGGATTGGATTGGACT+CGG 0.527154 7:-58199500 MsG0780039117.01.T01:CDS
TTAAGTATCAACAACTTGCA+TGG 0.532284 7:-58198978 MsG0780039117.01.T01:CDS
TATTAGATATATCAAAACTA+AGG 0.534942 7:-58199091 MsG0780039117.01.T01:intron
AATTGTTGAAACTAATGTCA+AGG 0.535895 7:+58199581 None:intergenic
TTTGTAAATTCAAACTCTTG+AGG 0.544827 7:+58199243 None:intergenic
ATTGTTGAAACTAATGTCAA+GGG 0.568424 7:+58199582 None:intergenic
AACAGTTGTCACTCAAGTCA+AGG 0.569892 7:+58199057 None:intergenic
TGCAAGGACAGTAATATCTA+GGG 0.574090 7:+58198953 None:intergenic
AATGAAGTTGCTACATCTGG+AGG 0.576012 7:-58199605 MsG0780039117.01.T01:CDS
GAAGTCATAGAGTACATAGC+AGG 0.577613 7:+58199794 None:intergenic
AATTTGGAAGTTCTTCACTT+GGG 0.579296 7:-58199893 MsG0780039117.01.T01:CDS
TCAAGAGTTTGAATTTACAA+AGG 0.583470 7:-58199241 MsG0780039117.01.T01:CDS
TTTGGTGACTCTTCCAGTGG+TGG 0.594419 7:+58200002 None:intergenic
TGAATCCGCTGACTGATGGC+AGG 0.597325 7:+58199545 None:intergenic
AGTTGTTGTTGGAGATGTCA+AGG 0.608673 7:+58199309 None:intergenic
GATGTGAATCCGCTGACTGA+TGG 0.637220 7:+58199541 None:intergenic
GAGTTTGGTGACTCTTCCAG+TGG 0.638376 7:+58199999 None:intergenic
ATTACTGTCCTTGCATGCAG+CGG 0.646001 7:-58198945 MsG0780039117.01.T01:CDS
AAGTCAAGAGATTTGAGACG+TGG 0.660772 7:+58198999 None:intergenic
GAAATCCTGCCATCAGTCAG+CGG 0.672334 7:-58199550 MsG0780039117.01.T01:CDS
GATCTCAAGATTCTTCAATG+AGG 0.693046 7:+58199331 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATGTAAATGATATTA+TGG + Chr7:58198965-58198984 None:intergenic 10.0%
!! ATATTATTAATTATTATGCA+GGG - Chr7:58198978-58198997 MsG0780039117.01.T01:CDS 10.0%
!! CTAAAAAAATTATTTGTATT+TGG + Chr7:58200009-58200028 None:intergenic 10.0%
!! AAATACAATTATTGTATACA+AGG - Chr7:58199503-58199522 MsG0780039117.01.T01:CDS 15.0%
!! CATATTATTAATTATTATGC+AGG - Chr7:58198977-58198996 MsG0780039117.01.T01:CDS 15.0%
!! TATTAGATATATCAAAACTA+AGG - Chr7:58199809-58199828 MsG0780039117.01.T01:CDS 15.0%
!! AATATTATTTCATCCCTAAA+TGG - Chr7:58199874-58199893 MsG0780039117.01.T01:CDS 20.0%
!! ATATTATGGTTATTATGTCA+AGG + Chr7:58198951-58198970 None:intergenic 20.0%
! AATTGTTGAAACTAATGTCA+AGG + Chr7:58199322-58199341 None:intergenic 25.0%
! ATTGTTGAAACTAATGTCAA+GGG + Chr7:58199321-58199340 None:intergenic 25.0%
! TATATCAAAACTAAGGTCTT+TGG - Chr7:58199816-58199835 MsG0780039117.01.T01:CDS 25.0%
! TCAAGAGTTTGAATTTACAA+AGG - Chr7:58199659-58199678 MsG0780039117.01.T01:intron 25.0%
! TTAAGAAAGAAAACTATAGC+TGG + Chr7:58199151-58199170 None:intergenic 25.0%
! TTGTAAATTCAAACTCTTGA+GGG + Chr7:58199659-58199678 None:intergenic 25.0%
! TTTGTAAATTCAAACTCTTG+AGG + Chr7:58199660-58199679 None:intergenic 25.0%
AATTTGGAAGTTCTTCACTT+GGG - Chr7:58199007-58199026 MsG0780039117.01.T01:CDS 30.0%
CATAACAGTCTGAAAATCTA+TGG + Chr7:58199264-58199283 None:intergenic 30.0%
TAATTTGGAAGTTCTTCACT+TGG - Chr7:58199006-58199025 MsG0780039117.01.T01:CDS 30.0%
TACTCTATGACTTCATTTCA+CGG - Chr7:58199115-58199134 MsG0780039117.01.T01:intron 30.0%
TCTAAATATATGAGACAAGC+AGG + Chr7:58199468-58199487 None:intergenic 30.0%
TTAAGTATCAACAACTTGCA+TGG - Chr7:58199922-58199941 MsG0780039117.01.T01:intron 30.0%
TTATGCAGGGTTGAATAATT+TGG - Chr7:58198991-58199010 MsG0780039117.01.T01:CDS 30.0%
! AAGCTAGAGAAATTTTTACG+AGG - Chr7:58199980-58199999 MsG0780039117.01.T01:intron 30.0%
! AGCTAGAGAAATTTTTACGA+GGG - Chr7:58199981-58200000 MsG0780039117.01.T01:intron 30.0%
! CATAGATTTTCAGACTGTTA+TGG - Chr7:58199262-58199281 MsG0780039117.01.T01:CDS 30.0%
ATGCAAGGACAGTAATATCT+AGG + Chr7:58199951-58199970 None:intergenic 35.0%
GATCTCAAGATTCTTCAATG+AGG + Chr7:58199572-58199591 None:intergenic 35.0%
TGCAAGGACAGTAATATCTA+GGG + Chr7:58199950-58199969 None:intergenic 35.0%
TTCAATGAAGTTGCTACATC+TGG - Chr7:58199292-58199311 MsG0780039117.01.T01:CDS 35.0%
! ATCTTTGAAGGAACTCCATT+TGG - Chr7:58199379-58199398 MsG0780039117.01.T01:intron 35.0%
! CTAGAGAAATTTTTACGAGG+GGG - Chr7:58199983-58200002 MsG0780039117.01.T01:intron 35.0%
! GCTAGAGAAATTTTTACGAG+GGG - Chr7:58199982-58200001 MsG0780039117.01.T01:intron 35.0%
! TATCAGCATTCTGTCTTGTT+TGG - Chr7:58199051-58199070 MsG0780039117.01.T01:CDS 35.0%
!! AGTGTCTTTGAAGTTGTTGT+TGG + Chr7:58199605-58199624 None:intergenic 35.0%
AAAGAAAACTATAGCTGGCC+TGG + Chr7:58199146-58199165 None:intergenic 40.0%
AACAGTTGTCACTCAAGTCA+AGG + Chr7:58199846-58199865 None:intergenic 40.0%
AATGAAGTTGCTACATCTGG+AGG - Chr7:58199295-58199314 MsG0780039117.01.T01:CDS 40.0%
ATGGGGTAGAAGAATGAGTT+TGG + Chr7:58198919-58198938 None:intergenic 40.0%
ATGTCAAGGTCCTTCGATAT+GGG + Chr7:58198937-58198956 None:intergenic 40.0%
ATTCTTCTACCCCATATCGA+AGG - Chr7:58198924-58198943 MsG0780039117.01.T01:CDS 40.0%
CATAGAGTACATAGCAGGTA+AGG + Chr7:58199104-58199123 None:intergenic 40.0%
CCAATCCAATCCAATCCAAA+TGG + Chr7:58199397-58199416 None:intergenic 40.0%
GAAGTCATAGAGTACATAGC+AGG + Chr7:58199109-58199128 None:intergenic 40.0%
GATATTGCATCAGCTCTTAG+TGG - Chr7:58199625-58199644 MsG0780039117.01.T01:CDS 40.0%
TATGACTTCATTTCACGGTG+AGG - Chr7:58199120-58199139 MsG0780039117.01.T01:intron 40.0%
TATGTCAAGGTCCTTCGATA+TGG + Chr7:58198938-58198957 None:intergenic 40.0%
TCAAACTCTTGAGGGAAGAA+AGG + Chr7:58199651-58199670 None:intergenic 40.0%
TGAAGGAACTCCATTTGGAT+TGG - Chr7:58199384-58199403 MsG0780039117.01.T01:intron 40.0%
! AAGTCAAGAGATTTGAGACG+TGG + Chr7:58199904-58199923 None:intergenic 40.0%
! AGTTGTTGTTGGAGATGTCA+AGG + Chr7:58199594-58199613 None:intergenic 40.0%
! CCATTTGGATTGGATTGGAT+TGG - Chr7:58199394-58199413 MsG0780039117.01.T01:intron 40.0%
! GAACTCCATTTGGATTGGAT+TGG - Chr7:58199389-58199408 MsG0780039117.01.T01:intron 40.0%
! GACTCGGATCTTCATTTTCA+AGG - Chr7:58199416-58199435 MsG0780039117.01.T01:intron 40.0%
!! AGCATTCTGTCTTGTTTGGA+TGG - Chr7:58199055-58199074 MsG0780039117.01.T01:CDS 40.0%
ATTACTGTCCTTGCATGCAG+CGG - Chr7:58199955-58199974 MsG0780039117.01.T01:intron 45.0%
GAGACGTGGAAGACCATTTA+GGG + Chr7:58199890-58199909 None:intergenic 45.0%
TGAGACGTGGAAGACCATTT+AGG + Chr7:58199891-58199910 None:intergenic 45.0%
TGTCAAGGTCCTTCGATATG+GGG + Chr7:58198936-58198955 None:intergenic 45.0%
! CAGCGGATTCACATCTTTGA+AGG - Chr7:58199367-58199386 MsG0780039117.01.T01:intron 45.0%
! GGATTGGATTGGATTGGACT+CGG - Chr7:58199400-58199419 MsG0780039117.01.T01:intron 45.0%
GAAATCCTGCCATCAGTCAG+CGG - Chr7:58199350-58199369 MsG0780039117.01.T01:intron 50.0%
! GAGTTTGGTGACTCTTCCAG+TGG + Chr7:58198904-58198923 None:intergenic 50.0%
! GATGTGAATCCGCTGACTGA+TGG + Chr7:58199362-58199381 None:intergenic 50.0%
! TTTGGTGACTCTTCCAGTGG+TGG + Chr7:58198901-58198920 None:intergenic 50.0%
CTTCATTTCACGGTGAGGCC+AGG - Chr7:58199125-58199144 MsG0780039117.01.T01:intron 55.0%
! TGAATCCGCTGACTGATGGC+AGG + Chr7:58199358-58199377 None:intergenic 55.0%
TAGCTTCGCCGCTGCATGCA+AGG + Chr7:58199966-58199985 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 58198890 58200032 58198890 ID=MsG0780039117.01;Name=MsG0780039117.01
Chr7 mRNA 58198890 58200032 58198890 ID=MsG0780039117.01.T01;Parent=MsG0780039117.01;Name=MsG0780039117.01.T01;_AED=0.52;_eAED=0.53;_QI=0|0.75|0.4|1|0.75|0.8|5|0|232
Chr7 exon 58200011 58200032 58200011 ID=MsG0780039117.01.T01:exon:6657;Parent=MsG0780039117.01.T01
Chr7 exon 58199786 58199923 58199786 ID=MsG0780039117.01.T01:exon:6656;Parent=MsG0780039117.01.T01
Chr7 exon 58199485 58199655 58199485 ID=MsG0780039117.01.T01:exon:6655;Parent=MsG0780039117.01.T01
Chr7 exon 58199213 58199362 58199213 ID=MsG0780039117.01.T01:exon:6654;Parent=MsG0780039117.01.T01
Chr7 exon 58198890 58199107 58198890 ID=MsG0780039117.01.T01:exon:6653;Parent=MsG0780039117.01.T01
Chr7 CDS 58200011 58200032 58200011 ID=MsG0780039117.01.T01:cds;Parent=MsG0780039117.01.T01
Chr7 CDS 58199786 58199923 58199786 ID=MsG0780039117.01.T01:cds;Parent=MsG0780039117.01.T01
Chr7 CDS 58199485 58199655 58199485 ID=MsG0780039117.01.T01:cds;Parent=MsG0780039117.01.T01
Chr7 CDS 58199213 58199362 58199213 ID=MsG0780039117.01.T01:cds;Parent=MsG0780039117.01.T01
Chr7 CDS 58198890 58199107 58198890 ID=MsG0780039117.01.T01:cds;Parent=MsG0780039117.01.T01
Gene Sequence

>MsG0780039117.01.T01

ATGCAACACCACCACTGGAAGAGGTTGAATAATTTGGAAGTTCTTCACTTGGGTTTCAATCTCTTCGATAATGCTATCAGCATTCTGTCTTGTTTGGATGGTCTTTCTTCTCTGAAATCTCTAACCTTACCTGCTATGTACTCTATGACTTCATTTCACGATTTTCAGACTGTTATGGAGACAATTTCAATGAAGTTGCTACATCTGGAGGCCCTTGACATTAGTTTCAACAATTTGAGTAATGAAATCCTGCCATCAGTCAGCGGATTCACATCTTTGAAGGAACTCCATTTGGATTGGATTGGATTGGACTCGGATCTTCATTTTCAAGCATTATGCGCCTCATTGAAGAATCTTGAGATCCTTGACATCTCCAACAACAACTTCAAAGACACTGATATTGCATCAGCTCTTAGTGGCCTTTCTTCCCTCAAGAGTTTGAATTTACAAAGGAGTGAACTTACTATGAGATCAATTCATAATATATCAAAACTAAGGTCTTTGGAGATCCTTGACTTGAGTGACAACTGTTTAAGTAACAATATTATTTCATCCCTAAATGGTCTTCCACGTCTCAAATCTCTTGACTTAAGTATCAACAACTTGCATGGATCCCTAGATATTACTGTCCTTGCATGCAGCGGCGAAGCTAGAGAAATTTTTACGAGGGGGCCAAATACAAATAATTTTTTTAGATAA

Protein sequence

>MsG0780039117.01.T01

MQHHHWKRLNNLEVLHLGFNLFDNAISILSCLDGLSSLKSLTLPAMYSMTSFHDFQTVMETISMKLLHLEALDISFNNLSNEILPSVSGFTSLKELHLDWIGLDSDLHFQALCASLKNLEILDISNNNFKDTDIASALSGLSSLKSLNLQRSELTMRSIHNISKLRSLEILDLSDNCLSNNIISSLNGLPRLKSLDLSINNLHGSLDITVLACSGEAREIFTRGPNTNNFFR*