Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048311.01.T01 | KEH36453.1 | 78.095 | 105 | 16 | 2 | 1 | 105 | 1 | 98 | 1.16E-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048311.01.T01 | A0A072V541 | 78.095 | 105 | 16 | 2 | 1 | 105 | 1 | 98 | 5.55e-23 | 94.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048311.01 | MsG0080048781.01 | 0.879049 | 1.710836e-69 | 9.140211e-66 |
| MsG0080048311.01 | MsG0080049140.01 | 0.849416 | 3.278527e-60 | 6.210524e-57 |
| MsG0080048311.01 | MsG0180000111.01 | 0.821394 | 4.134076e-53 | 3.359181e-50 |
| MsG0080048311.01 | MsG0180002421.01 | 0.852566 | 4.243596e-61 | 8.903394e-58 |
| MsG0080048311.01 | MsG0180003427.01 | 0.809426 | 1.903724e-50 | 1.117393e-47 |
| MsG0080048311.01 | MsG0180003485.01 | 0.822297 | 2.554638e-53 | 2.129708e-50 |
| MsG0080048311.01 | MsG0180003658.01 | 0.815549 | 8.732365e-52 | 6.038934e-49 |
| MsG0080048311.01 | MsG0180003796.01 | 0.835554 | 1.568405e-56 | 1.924501e-53 |
| MsG0080048311.01 | MsG0180004126.01 | 0.814509 | 1.485870e-51 | 9.989806e-49 |
| MsG0080048311.01 | MsG0180005435.01 | 0.808181 | 3.514138e-50 | 1.995844e-47 |
| MsG0080048311.01 | MsG0180006133.01 | 0.800630 | 1.316719e-48 | 6.161044e-46 |
| MsG0080048311.01 | MsG0280006477.01 | 0.932488 | 7.900447e-95 | 5.308303e-90 |
| MsG0080048311.01 | MsG0280006540.01 | 0.830952 | 2.197050e-55 | 2.350107e-52 |
| MsG0080048311.01 | MsG0280006563.01 | 0.826020 | 3.411792e-54 | 3.161808e-51 |
| MsG0080048311.01 | MsG0280007359.01 | 0.810990 | 8.757117e-51 | 5.357731e-48 |
| MsG0080048311.01 | MsG0280009752.01 | 0.823813 | 1.131342e-53 | 9.846062e-51 |
| MsG0080048311.01 | MsG0280010579.01 | 0.802742 | 4.856453e-49 | 2.397492e-46 |
| MsG0080048311.01 | MsG0280010779.01 | 0.811750 | 5.988666e-51 | 3.737676e-48 |
| MsG0080048311.01 | MsG0280010977.01 | 0.806899 | 6.575324e-50 | 3.611348e-47 |
| MsG0080048311.01 | MsG0280011154.01 | 0.821483 | 3.941366e-53 | 3.210598e-50 |
| MsG0080048311.01 | MsG0380015548.01 | 0.856901 | 2.351122e-62 | 5.698243e-59 |
| MsG0080048311.01 | MsG0380015816.01 | 0.803371 | 3.599736e-49 | 1.805751e-46 |
| MsG0080048311.01 | MsG0380016091.01 | 0.824727 | 6.900788e-54 | 6.164242e-51 |
| MsG0080048311.01 | MsG0380016225.01 | 0.808151 | 3.564775e-50 | 2.023028e-47 |
| MsG0080048311.01 | MsG0380016703.01 | 0.818016 | 2.442137e-52 | 1.806902e-49 |
| MsG0080048311.01 | MsG0380017361.01 | 0.859234 | 4.762696e-63 | 1.249016e-59 |
| MsG0080048311.01 | MsG0480018526.01 | 0.851291 | 9.764288e-61 | 1.967040e-57 |
| MsG0080048311.01 | MsG0480019635.01 | 0.800190 | 1.618723e-48 | 7.489840e-46 |
| MsG0080048311.01 | MsG0480021013.01 | 0.800847 | 1.189547e-48 | 5.596722e-46 |
| MsG0080048311.01 | MsG0480022331.01 | 0.831798 | 1.360989e-55 | 1.492962e-52 |
| MsG0080048311.01 | MsG0480023262.01 | 0.806419 | 8.303533e-50 | 4.504596e-47 |
| MsG0080048311.01 | MsG0480023296.01 | 0.830189 | 3.377975e-55 | 3.533686e-52 |
| MsG0080048311.01 | MsG0480023672.01 | 0.841603 | 4.298432e-58 | 6.349017e-55 |
| MsG0080048311.01 | MsG0580024657.01 | 0.800380 | 1.481196e-48 | 6.886851e-46 |
| MsG0080048311.01 | MsG0580024787.01 | 0.821671 | 3.567453e-53 | 2.921354e-50 |
| MsG0080048311.01 | MsG0580024813.01 | 0.813679 | 2.265470e-51 | 1.489160e-48 |
| MsG0080048311.01 | MsG0580024954.01 | 0.820667 | 6.075927e-53 | 4.837898e-50 |
| MsG0080048311.01 | MsG0580025093.01 | 0.824761 | 6.775204e-54 | 6.057864e-51 |
| MsG0080048311.01 | MsG0580025775.01 | 0.815179 | 1.055303e-51 | 7.223898e-49 |
| MsG0080048311.01 | MsG0580026186.01 | 0.802331 | 5.901099e-49 | 2.882940e-46 |
| MsG0080048311.01 | MsG0580028756.01 | 0.834406 | 3.052468e-56 | 3.619163e-53 |
| MsG0080048311.01 | MsG0580029261.01 | 0.825700 | 4.063413e-54 | 3.731538e-51 |
| MsG0080048311.01 | MsG0580029298.01 | 0.845652 | 3.553183e-59 | 5.962897e-56 |
| MsG0080048311.01 | MsG0580029591.01 | 0.838769 | 2.362609e-57 | 3.196542e-54 |
| MsG0080048311.01 | MsG0680031616.01 | 0.823210 | 1.565912e-53 | 1.339621e-50 |
| MsG0080048311.01 | MsG0680033696.01 | 0.819180 | 1.329678e-52 | 1.015908e-49 |
| MsG0080048311.01 | MsG0780037741.01 | 0.804046 | 2.606465e-49 | 1.330475e-46 |
| MsG0080048311.01 | MsG0780038402.01 | 0.803522 | 3.348695e-49 | 1.686521e-46 |
| MsG0080048311.01 | MsG0780039861.01 | 0.859750 | 3.332486e-63 | 8.896306e-60 |
| MsG0080048311.01 | MsG0780040364.01 | 0.835253 | 1.868303e-56 | 2.272241e-53 |
| MsG0080048311.01 | MsG0780040543.01 | 0.800205 | 1.607647e-48 | 7.441422e-46 |
| MsG0080048311.01 | MsG0780040620.01 | 0.806265 | 8.947924e-50 | 4.835492e-47 |
| MsG0080048311.01 | MsG0780041063.01 | 0.828851 | 7.145020e-55 | 7.186910e-52 |
| MsG0080048311.01 | MsG0780041141.01 | 0.817201 | 3.727599e-52 | 2.697102e-49 |
| MsG0080048311.01 | MsG0780041143.01 | 0.860562 | 1.893984e-63 | 5.199497e-60 |
| MsG0080048311.01 | MsG0780041340.01 | 0.817737 | 2.822956e-52 | 2.072832e-49 |
| MsG0080048311.01 | MsG0780041756.01 | 0.815898 | 7.300916e-52 | 5.097190e-49 |
| MsG0080048311.01 | MsG0880045552.01 | 0.858578 | 7.485080e-63 | 1.919191e-59 |
| MsG0080048311.01 | MsG0880045584.01 | 0.804594 | 2.004646e-49 | 1.037746e-46 |
| MsG0080048311.01 | MsG0880045612.01 | 0.838330 | 3.065580e-57 | 4.092261e-54 |
| MsG0080048311.01 | MsG0880046208.01 | 0.811395 | 7.153289e-51 | 4.422922e-48 |
| MsG0080048311.01 | MsG0880046431.01 | 0.808725 | 2.689088e-50 | 1.549044e-47 |
| MsG0080048311.01 | MsG0880046646.01 | 0.807959 | 3.917704e-50 | 2.211956e-47 |
| MsG0080048311.01 | MsG0880046885.01 | 0.826609 | 2.469781e-54 | 2.327389e-51 |
| MsG0080048311.01 | MsG0880047226.01 | 0.804769 | 1.842231e-49 | 9.580543e-47 |
| MsG0080048311.01 | MsG0880047649.01 | 0.819666 | 1.030417e-52 | 7.979799e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048311.01.T01 | MTR_2g009275 | 78.095 | 105 | 16 | 2 | 1 | 105 | 1 | 98 | 1.41e-26 | 94.7 |
| MsG0080048311.01.T01 | MTR_2g009150 | 46.667 | 105 | 41 | 4 | 1 | 104 | 1 | 91 | 8.21e-16 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCTGTTGCTGCTACTGC+TGG | 0.364799 | contig271end:-135998 | MsG0080048311.01.T01:CDS |
| GTCTCTCCTTCTTCCATCTC+TGG | 0.367654 | contig271end:-136058 | MsG0080048311.01.T01:CDS |
| TTTCCTTGGCGACTGTGATT+TGG | 0.378125 | contig271end:+136156 | MsG0080048310.01.T01:CDS |
| GGTGAAGTAGCGGATGGTTC+AGG | 0.402991 | contig271end:+136085 | MsG0080048310.01.T01:CDS |
| GGAGGAGAATTTCCAGTGGC+AGG | 0.448322 | contig271end:+136106 | MsG0080048310.01.T01:CDS |
| GATGGGGAGATTGCTTTCCT+TGG | 0.456249 | contig271end:+136142 | MsG0080048310.01.T01:CDS |
| TACAACAATGGCTCAATCTC+CGG | 0.456813 | contig271end:-136191 | MsG0080048311.01.T01:CDS |
| CAGGAGAAGACGCCGGTGAT+GGG | 0.475687 | contig271end:+136125 | MsG0080048310.01.T01:CDS |
| GTTGTTGAGCACTACAACAA+TGG | 0.476326 | contig271end:-136203 | MsG0080048311.01.T01:CDS |
| GCAGGAGAAGACGCCGGTGA+TGG | 0.484603 | contig271end:+136124 | MsG0080048310.01.T01:CDS |
| ACGGCACTGGAAGCAGGTCC+TGG | 0.494599 | contig271end:+136019 | MsG0080048310.01.T01:CDS |
| GAACATTGCTCTTATGATTG+TGG | 0.500197 | contig271end:-136233 | MsG0080048311.01.T01:CDS |
| ACAGAGACGGCACTGGAAGC+AGG | 0.508633 | contig271end:+136013 | MsG0080048310.01.T01:CDS |
| GGTCCTGGTGCTTCAGATGG+TGG | 0.509452 | contig271end:+136034 | MsG0080048310.01.T01:CDS |
| AGCAGCAACAGAGACGGCAC+TGG | 0.519764 | contig271end:+136006 | MsG0080048310.01.T01:CDS |
| CCGGCGTCTTCTCCTGCCAC+TGG | 0.519854 | contig271end:-136118 | MsG0080048311.01.T01:CDS |
| GCAGGTCCTGGTGCTTCAGA+TGG | 0.523119 | contig271end:+136031 | MsG0080048310.01.T01:CDS |
| CTTGGCGACTGTGATTTGGT+TGG | 0.533471 | contig271end:+136160 | MsG0080048310.01.T01:CDS |
| GGTGGACCAGAGATGGAAGA+AGG | 0.548296 | contig271end:+136052 | MsG0080048310.01.T01:CDS |
| AGGAGAAGACGCCGGTGATG+GGG | 0.563301 | contig271end:+136126 | MsG0080048310.01.T01:CDS |
| GAAAGCAATCTCCCCATCAC+CGG | 0.563344 | contig271end:-136137 | MsG0080048311.01.T01:CDS |
| TGAACCATCCGCTACTTCAC+CGG | 0.570119 | contig271end:-136083 | MsG0080048311.01.T01:CDS |
| TTCAGATGGTGGACCAGAGA+TGG | 0.580542 | contig271end:+136045 | MsG0080048310.01.T01:CDS |
| ATGGAAGAAGGAGAGACAGC+CGG | 0.582727 | contig271end:+136064 | MsG0080048310.01.T01:CDS |
| AGAGACAGCCGGTGAAGTAG+CGG | 0.584069 | contig271end:+136075 | MsG0080048310.01.T01:CDS |
| CAACCAAATCACAGTCGCCA+AGG | 0.590381 | contig271end:-136159 | MsG0080048311.01.T01:CDS |
| GATTTGGTTGGCGATGAAGC+CGG | 0.598616 | contig271end:+136172 | MsG0080048310.01.T01:CDS |
| GGTCCACCATCTGAAGCACC+AGG | 0.613375 | contig271end:-136037 | MsG0080048311.01.T01:CDS |
| AGCAGTAGCAGCAACAGAGA+CGG | 0.613692 | contig271end:+136000 | MsG0080048310.01.T01:CDS |
| GAAGTAGCGGATGGTTCAGG+AGG | 0.667793 | contig271end:+136088 | MsG0080048310.01.T01:CDS |
| ACAGCCGGTGAAGTAGCGGA+TGG | 0.678585 | contig271end:+136079 | MsG0080048310.01.T01:CDS |
| TTCAGGAGGAGAATTTCCAG+TGG | 0.715472 | contig271end:+136102 | MsG0080048310.01.T01:CDS |
| CCAGTGGCAGGAGAAGACGC+CGG | 0.748622 | contig271end:+136118 | MsG0080048310.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAACATTGCTCTTATGATTG+TGG | - | contig271end:135972-135991 | MsG0080048311.01.T01:CDS | 35.0% | |
| !!! | TGCTATTGTCATTTTCGTAG+CGG | - | contig271end:136236-136255 | MsG0080048311.01.T01:CDS | 35.0% |
| GTTGTTGAGCACTACAACAA+TGG | - | contig271end:136002-136021 | MsG0080048311.01.T01:CDS | 40.0% | |
| TACAACAATGGCTCAATCTC+CGG | - | contig271end:136014-136033 | MsG0080048311.01.T01:CDS | 40.0% | |
| TTCAGGAGGAGAATTTCCAG+TGG | + | contig271end:136106-136125 | MsG0080048310.01.T01:CDS | 45.0% | |
| TTTCCTTGGCGACTGTGATT+TGG | + | contig271end:136052-136071 | MsG0080048310.01.T01:CDS | 45.0% | |
| AGCAGTAGCAGCAACAGAGA+CGG | + | contig271end:136208-136227 | None:intergenic | 50.0% | |
| ATGGAAGAAGGAGAGACAGC+CGG | + | contig271end:136144-136163 | MsG0080048310.01.T01:CDS | 50.0% | |
| CAACCAAATCACAGTCGCCA+AGG | - | contig271end:136046-136065 | MsG0080048311.01.T01:CDS | 50.0% | |
| GAAAGCAATCTCCCCATCAC+CGG | - | contig271end:136068-136087 | MsG0080048311.01.T01:CDS | 50.0% | |
| GATGGGGAGATTGCTTTCCT+TGG | + | contig271end:136066-136085 | MsG0080048310.01.T01:CDS | 50.0% | |
| GTCTCTCCTTCTTCCATCTC+TGG | - | contig271end:136147-136166 | MsG0080048311.01.T01:CDS | 50.0% | |
| TGAACCATCCGCTACTTCAC+CGG | - | contig271end:136122-136141 | MsG0080048311.01.T01:CDS | 50.0% | |
| ! | GATTTGGTTGGCGATGAAGC+CGG | + | contig271end:136036-136055 | MsG0080048310.01.T01:CDS | 50.0% |
| !! | CTTGGCGACTGTGATTTGGT+TGG | + | contig271end:136048-136067 | MsG0080048310.01.T01:CDS | 50.0% |
| !! | TTCAGATGGTGGACCAGAGA+TGG | + | contig271end:136163-136182 | MsG0080048310.01.T01:CDS | 50.0% |
| AGAGACAGCCGGTGAAGTAG+CGG | + | contig271end:136133-136152 | MsG0080048310.01.T01:CDS | 55.0% | |
| GGAGGAGAATTTCCAGTGGC+AGG | + | contig271end:136102-136121 | MsG0080048310.01.T01:CDS | 55.0% | |
| GGTGGACCAGAGATGGAAGA+AGG | + | contig271end:136156-136175 | MsG0080048310.01.T01:CDS | 55.0% | |
| GTCTCTGTTGCTGCTACTGC+TGG | - | contig271end:136207-136226 | MsG0080048311.01.T01:CDS | 55.0% | |
| !! | GAAGTAGCGGATGGTTCAGG+AGG | + | contig271end:136120-136139 | MsG0080048310.01.T01:CDS | 55.0% |
| !! | GGTGAAGTAGCGGATGGTTC+AGG | + | contig271end:136123-136142 | MsG0080048310.01.T01:CDS | 55.0% |
| AGCAGCAACAGAGACGGCAC+TGG | + | contig271end:136202-136221 | None:intergenic | 60.0% | |
| AGGAGAAGACGCCGGTGATG+GGG | + | contig271end:136082-136101 | MsG0080048310.01.T01:CDS | 60.0% | |
| CAGGAGAAGACGCCGGTGAT+GGG | + | contig271end:136083-136102 | MsG0080048310.01.T01:CDS | 60.0% | |
| GCAGGTCCTGGTGCTTCAGA+TGG | + | contig271end:136177-136196 | MsG0080048310.01.T01:CDS | 60.0% | |
| GGTCCTGGTGCTTCAGATGG+TGG | + | contig271end:136174-136193 | MsG0080048310.01.T01:CDS | 60.0% | |
| ! | ACAGAGACGGCACTGGAAGC+AGG | + | contig271end:136195-136214 | None:intergenic | 60.0% |
| ! | ACAGCCGGTGAAGTAGCGGA+TGG | + | contig271end:136129-136148 | MsG0080048310.01.T01:CDS | 60.0% |
| ! | GGTCCACCATCTGAAGCACC+AGG | - | contig271end:136168-136187 | MsG0080048311.01.T01:CDS | 60.0% |
| CCAGTGGCAGGAGAAGACGC+CGG | + | contig271end:136090-136109 | MsG0080048310.01.T01:CDS | 65.0% | |
| GCAGGAGAAGACGCCGGTGA+TGG | + | contig271end:136084-136103 | MsG0080048310.01.T01:CDS | 65.0% | |
| ! | ACGGCACTGGAAGCAGGTCC+TGG | + | contig271end:136189-136208 | None:intergenic | 65.0% |
| CCGGCGTCTTCTCCTGCCAC+TGG | - | contig271end:136087-136106 | MsG0080048311.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig271end | gene | 135955 | 136272 | 135955 | ID=MsG0080048311.01;Name=MsG0080048311.01 |
| contig271end | mRNA | 135955 | 136272 | 135955 | ID=MsG0080048311.01.T01;Parent=MsG0080048311.01;Name=MsG0080048311.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|105 |
| contig271end | exon | 135955 | 136272 | 135955 | ID=MsG0080048311.01.T01:exon:2851;Parent=MsG0080048311.01.T01 |
| contig271end | CDS | 135955 | 136272 | 135955 | ID=MsG0080048311.01.T01:cds;Parent=MsG0080048311.01.T01 |
| Gene Sequence |
| Protein sequence |
