Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039869.01.T01 | XP_027353411.1 | 48.571 | 105 | 50 | 3 | 1 | 102 | 1 | 104 | 4.38E-18 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039869.01.T01 | A0A2P6S6E4 | 45.714 | 105 | 53 | 3 | 1 | 102 | 1 | 104 | 3.59e-16 | 78.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000585.01 | MsG0780039869.01 | 0.800884 | 1.169018e-48 | 5.505419e-46 |
| MsG0180001922.01 | MsG0780039869.01 | 0.807628 | 4.606576e-50 | 2.578461e-47 |
| MsG0380011754.01 | MsG0780039869.01 | 0.804759 | 1.851705e-49 | 9.627267e-47 |
| MsG0480019289.01 | MsG0780039869.01 | 0.811248 | 7.698984e-51 | 4.741865e-48 |
| MsG0480020487.01 | MsG0780039869.01 | 0.801984 | 6.956561e-49 | 3.368327e-46 |
| MsG0480021900.01 | MsG0780039869.01 | 0.807985 | 3.867180e-50 | 2.184932e-47 |
| MsG0280006935.01 | MsG0780039869.01 | 0.802345 | 5.863739e-49 | 2.865640e-46 |
| MsG0280007048.01 | MsG0780039869.01 | 0.803451 | 3.464325e-49 | 1.741501e-46 |
| MsG0280007948.01 | MsG0780039869.01 | 0.803831 | 2.889417e-49 | 1.466772e-46 |
| MsG0780039869.01 | MsG0780039875.01 | 0.811483 | 6.844564e-51 | 4.241966e-48 |
| MsG0780039869.01 | MsG0780040679.01 | 0.816749 | 4.710220e-52 | 3.366104e-49 |
| MsG0780039869.01 | MsG0780040758.01 | 0.804610 | 1.988559e-49 | 1.029864e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780039869.01.T01 | MTR_6g023460 | 40.952 | 105 | 58 | 3 | 1 | 102 | 3 | 106 | 5.51e-16 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGTTACTTTGATGTAAA+TGG | 0.224758 | 7:-69921633 | MsG0780039869.01.T01:CDS |
| CGAGGGAACAAGGAAGATAA+AGG | 0.383866 | 7:-69921462 | MsG0780039869.01.T01:CDS |
| GTTATCTGTGGAGGATTTAG+AGG | 0.411714 | 7:-69921553 | MsG0780039869.01.T01:CDS |
| CTAGACGGTGATGAACTGTT+GGG | 0.455821 | 7:-69921516 | MsG0780039869.01.T01:CDS |
| TAAAGGGCAAAGCTTTAAAG+AGG | 0.495939 | 7:-69921445 | MsG0780039869.01.T01:CDS |
| AATTAATATGCCTCTTGAGA+TGG | 0.502884 | 7:+69921379 | None:intergenic |
| CGGAGATTTGTGGAAGCTCG+AGG | 0.524244 | 7:-69921480 | MsG0780039869.01.T01:CDS |
| CCTCCACAGATAACTCCTCA+TGG | 0.528422 | 7:+69921562 | None:intergenic |
| TCTAGACGGTGATGAACTGT+TGG | 0.531424 | 7:-69921517 | MsG0780039869.01.T01:CDS |
| GGAGGATTTAGAGGCGGTTG+AGG | 0.533575 | 7:-69921544 | MsG0780039869.01.T01:CDS |
| GAGGGAACAAGGAAGATAAA+GGG | 0.552009 | 7:-69921461 | MsG0780039869.01.T01:CDS |
| TTGATCAGTTCCATCTCAAG+AGG | 0.560717 | 7:-69921389 | MsG0780039869.01.T01:CDS |
| TGTGGAAGCTCGAGGGAACA+AGG | 0.565881 | 7:-69921472 | MsG0780039869.01.T01:CDS |
| TGATCAAATAGACTCACCTC+AGG | 0.578172 | 7:+69921405 | None:intergenic |
| CACAGATAACTCCTCATGGA+TGG | 0.590113 | 7:+69921566 | None:intergenic |
| TAGACGGTGATGAACTGTTG+GGG | 0.595417 | 7:-69921515 | MsG0780039869.01.T01:CDS |
| TGAATTGCAAAATTGCATGA+AGG | 0.597499 | 7:-69921589 | MsG0780039869.01.T01:CDS |
| GGAGATTTGTGGAAGCTCGA+GGG | 0.611100 | 7:-69921479 | MsG0780039869.01.T01:CDS |
| GCGGTTGAGGCATTTCTAGA+CGG | 0.612643 | 7:-69921531 | MsG0780039869.01.T01:CDS |
| ATATATGGTTATTATGGACA+AGG | 0.627190 | 7:-69921676 | None:intergenic |
| ATCTGTGGAGGATTTAGAGG+CGG | 0.627233 | 7:-69921550 | MsG0780039869.01.T01:CDS |
| CCATGAGGAGTTATCTGTGG+AGG | 0.646843 | 7:-69921562 | MsG0780039869.01.T01:CDS |
| CATCCATGAGGAGTTATCTG+TGG | 0.683615 | 7:-69921565 | MsG0780039869.01.T01:CDS |
| TTGCATGAAGGCCATCCATG+AGG | 0.708194 | 7:-69921577 | MsG0780039869.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAAGAGGTTTTTAATGTTT+TGG | - | Chr7:69921607-69921626 | MsG0780039869.01.T01:CDS | 20.0% |
| !! | TTCAGTTACTTTGATGTAAA+TGG | - | Chr7:69921404-69921423 | MsG0780039869.01.T01:CDS | 25.0% |
| AATTAATATGCCTCTTGAGA+TGG | + | Chr7:69921661-69921680 | None:intergenic | 30.0% | |
| TGAATTGCAAAATTGCATGA+AGG | - | Chr7:69921448-69921467 | MsG0780039869.01.T01:CDS | 30.0% | |
| !! | TTTTAATGTTTTGGATCCTG+AGG | - | Chr7:69921616-69921635 | MsG0780039869.01.T01:CDS | 30.0% |
| ! | TGATGATTTTCGGAGATTTG+TGG | - | Chr7:69921547-69921566 | MsG0780039869.01.T01:CDS | 35.0% |
| !! | TAAAGGGCAAAGCTTTAAAG+AGG | - | Chr7:69921592-69921611 | MsG0780039869.01.T01:CDS | 35.0% |
| !! | TGTTGGGGTTTGATGATTTT+CGG | - | Chr7:69921537-69921556 | MsG0780039869.01.T01:CDS | 35.0% |
| GAGGGAACAAGGAAGATAAA+GGG | - | Chr7:69921576-69921595 | MsG0780039869.01.T01:CDS | 40.0% | |
| GTTATCTGTGGAGGATTTAG+AGG | - | Chr7:69921484-69921503 | MsG0780039869.01.T01:CDS | 40.0% | |
| TGATCAAATAGACTCACCTC+AGG | + | Chr7:69921635-69921654 | None:intergenic | 40.0% | |
| TTGATCAGTTCCATCTCAAG+AGG | - | Chr7:69921648-69921667 | MsG0780039869.01.T01:CDS | 40.0% | |
| ATCTGTGGAGGATTTAGAGG+CGG | - | Chr7:69921487-69921506 | MsG0780039869.01.T01:CDS | 45.0% | |
| CACAGATAACTCCTCATGGA+TGG | + | Chr7:69921474-69921493 | None:intergenic | 45.0% | |
| CATCCATGAGGAGTTATCTG+TGG | - | Chr7:69921472-69921491 | MsG0780039869.01.T01:CDS | 45.0% | |
| CGAGGGAACAAGGAAGATAA+AGG | - | Chr7:69921575-69921594 | MsG0780039869.01.T01:CDS | 45.0% | |
| CTAGACGGTGATGAACTGTT+GGG | - | Chr7:69921521-69921540 | MsG0780039869.01.T01:CDS | 45.0% | |
| TAGACGGTGATGAACTGTTG+GGG | - | Chr7:69921522-69921541 | MsG0780039869.01.T01:CDS | 45.0% | |
| TCTAGACGGTGATGAACTGT+TGG | - | Chr7:69921520-69921539 | MsG0780039869.01.T01:CDS | 45.0% | |
| CCATGAGGAGTTATCTGTGG+AGG | - | Chr7:69921475-69921494 | MsG0780039869.01.T01:CDS | 50.0% | |
| CCTCCACAGATAACTCCTCA+TGG | + | Chr7:69921478-69921497 | None:intergenic | 50.0% | |
| GGAGATTTGTGGAAGCTCGA+GGG | - | Chr7:69921558-69921577 | MsG0780039869.01.T01:CDS | 50.0% | |
| TTGCATGAAGGCCATCCATG+AGG | - | Chr7:69921460-69921479 | MsG0780039869.01.T01:CDS | 50.0% | |
| ! | GCGGTTGAGGCATTTCTAGA+CGG | - | Chr7:69921506-69921525 | MsG0780039869.01.T01:CDS | 50.0% |
| CGGAGATTTGTGGAAGCTCG+AGG | - | Chr7:69921557-69921576 | MsG0780039869.01.T01:CDS | 55.0% | |
| GGAGGATTTAGAGGCGGTTG+AGG | - | Chr7:69921493-69921512 | MsG0780039869.01.T01:CDS | 55.0% | |
| TGTGGAAGCTCGAGGGAACA+AGG | - | Chr7:69921565-69921584 | MsG0780039869.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 69921374 | 69921685 | 69921374 | ID=MsG0780039869.01;Name=MsG0780039869.01 |
| Chr7 | mRNA | 69921374 | 69921685 | 69921374 | ID=MsG0780039869.01.T01;Parent=MsG0780039869.01;Name=MsG0780039869.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|103 |
| Chr7 | exon | 69921374 | 69921685 | 69921374 | ID=MsG0780039869.01.T01:exon:12934;Parent=MsG0780039869.01.T01 |
| Chr7 | CDS | 69921374 | 69921685 | 69921374 | ID=MsG0780039869.01.T01:cds;Parent=MsG0780039869.01.T01 |
| Gene Sequence |
| Protein sequence |
