Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040045.01.T01 | XP_003624466.2 | 89.326 | 178 | 13 | 2 | 1 | 178 | 1 | 172 | 5.32E-67 | 212 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040045.01.T01 | G7KW84 | 89.326 | 178 | 13 | 2 | 1 | 178 | 1 | 172 | 2.54e-67 | 212 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001710.01 | MsG0780040045.01 | 0.825559 | 4.387325e-54 | 4.012679e-51 |
| MsG0280011133.01 | MsG0780040045.01 | 0.820335 | 7.243166e-53 | 5.714671e-50 |
| MsG0380017788.01 | MsG0780040045.01 | 0.800768 | 1.234575e-48 | 5.796579e-46 |
| MsG0780040045.01 | MsG0880046449.01 | 0.802668 | 5.028990e-49 | 2.477902e-46 |
| MsG0780040045.01 | MsG0880046844.01 | 0.821000 | 5.094958e-53 | 4.095317e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040045.01.T01 | MTR_7g083640 | 89.326 | 178 | 13 | 2 | 1 | 178 | 1 | 172 | 6.44e-71 | 212 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040045.01.T01 | AT1G12830 | 68.750 | 80 | 22 | 1 | 91 | 170 | 116 | 192 | 8.85e-17 | 75.1 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCGTGGTTGTTGGTTT+CGG | 0.192971 | 7:+72416268 | None:intergenic |
| CGTCGATGAGGTTGGATTCT+TGG | 0.206975 | 7:+72416153 | None:intergenic |
| TCTTCGTGGTTGTTGGTTTC+GGG | 0.218487 | 7:+72416269 | None:intergenic |
| CAGACTGGAGTGCACATTTC+TGG | 0.227098 | 7:-72415786 | MsG0780040045.01.T01:CDS |
| GATTCTGACGGCAATGTTTC+GGG | 0.258012 | 7:-72415894 | MsG0780040045.01.T01:CDS |
| TCCACTTGCGGAGGTTGATT+TGG | 0.320599 | 7:-72415847 | MsG0780040045.01.T01:CDS |
| TGATTCTGACGGCAATGTTT+CGG | 0.357245 | 7:-72415895 | MsG0780040045.01.T01:CDS |
| TTCTTGATCGGGTTTGCGTT+TGG | 0.373289 | 7:+72416232 | None:intergenic |
| GTGGTGGGAGTAGTTATCTT+GGG | 0.392368 | 7:+72416182 | None:intergenic |
| AGGGATTATGCGCGATGATA+AGG | 0.420684 | 7:-72415988 | MsG0780040045.01.T01:CDS |
| GTGATGGGTTTCTTCTTGAT+CGG | 0.423416 | 7:+72416220 | None:intergenic |
| TTCGGGTTCGTTGTTGGGAA+CGG | 0.430062 | 7:+72416094 | None:intergenic |
| TGTGGGTTCTTCGTGGTTGT+TGG | 0.430236 | 7:+72416262 | None:intergenic |
| TGATGGGTTTCTTCTTGATC+GGG | 0.431013 | 7:+72416221 | None:intergenic |
| TTTCCCTTCCTGTCCACCAC+AGG | 0.432598 | 7:+72416011 | None:intergenic |
| TTGCGTTTGGTGGGGAATGT+GGG | 0.446781 | 7:+72416245 | None:intergenic |
| TGATCGGGTTTGCGTTTGGT+GGG | 0.446814 | 7:+72416236 | None:intergenic |
| GTGGTGGACAGGAAGGGAAA+AGG | 0.452668 | 7:-72416008 | MsG0780040045.01.T01:CDS |
| TTTGCGTTTGGTGGGGAATG+TGG | 0.460274 | 7:+72416244 | None:intergenic |
| GGGATTATGCGCGATGATAA+GGG | 0.461736 | 7:-72415987 | MsG0780040045.01.T01:CDS |
| ATTGACGGTGGTGGGAAGTT+CGG | 0.464983 | 7:+72416130 | None:intergenic |
| ACATTCTTCCGTCTAGGACT+CGG | 0.465560 | 7:-72415821 | MsG0780040045.01.T01:CDS |
| TTCGTTGTTGGGAACGGCGT+CGG | 0.465767 | 7:+72416100 | None:intergenic |
| TTGATCGGGTTTGCGTTTGG+TGG | 0.477903 | 7:+72416235 | None:intergenic |
| AGTGGTGGGAGTAGTTATCT+TGG | 0.478869 | 7:+72416181 | None:intergenic |
| ATGAAGAGCCTGTGGTGGAC+AGG | 0.489672 | 7:-72416019 | MsG0780040045.01.T01:CDS |
| TGCGCGATGATAAGGGAAAA+GGG | 0.491143 | 7:-72415980 | MsG0780040045.01.T01:CDS |
| TGGGGAATGTGGGTTCTTCG+TGG | 0.491435 | 7:+72416255 | None:intergenic |
| TGGTGGACAGGAAGGGAAAA+GGG | 0.491635 | 7:-72416007 | MsG0780040045.01.T01:CDS |
| ATGCGCGATGATAAGGGAAA+AGG | 0.491640 | 7:-72415981 | MsG0780040045.01.T01:CDS |
| GGATTCTTGGTCTACAGTGG+TGG | 0.497253 | 7:+72416166 | None:intergenic |
| CGGCGGCGAACTGCTCAGAC+TGG | 0.498986 | 7:-72415801 | MsG0780040045.01.T01:CDS |
| GATTCTTGGTCTACAGTGGT+GGG | 0.502202 | 7:+72416167 | None:intergenic |
| TGGTGGGAGTAGTTATCTTG+GGG | 0.509337 | 7:+72416183 | None:intergenic |
| AGTAGAATCTAATCAGCATT+CGG | 0.511857 | 7:+72415756 | None:intergenic |
| GGAAGAAGAATTGACGGTGG+TGG | 0.527818 | 7:+72416121 | None:intergenic |
| AAGTTCGGCGTCGATGAGGT+TGG | 0.528090 | 7:+72416145 | None:intergenic |
| TGGATAACATTCTTCCGTCT+AGG | 0.534730 | 7:-72415827 | MsG0780040045.01.T01:CDS |
| GATCGGGTTTGCGTTTGGTG+GGG | 0.540763 | 7:+72416237 | None:intergenic |
| GAAGAAGAATTGACGGTGGT+GGG | 0.545012 | 7:+72416122 | None:intergenic |
| AGAGCCTGTGGTGGACAGGA+AGG | 0.545443 | 7:-72416015 | MsG0780040045.01.T01:CDS |
| TCCAAATCAACCTCCGCAAG+TGG | 0.550043 | 7:+72415846 | None:intergenic |
| GGCGTCGGAAGAAGAATTGA+CGG | 0.559067 | 7:+72416115 | None:intergenic |
| GTCCACCACAGGCTCTTCAT+CGG | 0.559628 | 7:+72416022 | None:intergenic |
| AATGATTGAAGAAGAAGATG+AGG | 0.573565 | 7:-72415955 | MsG0780040045.01.T01:CDS |
| GGTGGGAGTAGTTATCTTGG+GGG | 0.592660 | 7:+72416184 | None:intergenic |
| GAGCCTGTGGTGGACAGGAA+GGG | 0.598333 | 7:-72416014 | MsG0780040045.01.T01:CDS |
| TTCTGATGATCCACTTGCGG+AGG | 0.598405 | 7:-72415856 | MsG0780040045.01.T01:CDS |
| GTTCGCCGCCGAGTCCTAGA+CGG | 0.608091 | 7:+72415813 | None:intergenic |
| TTCCGATGAAGAGCCTGTGG+TGG | 0.608098 | 7:-72416024 | MsG0780040045.01.T01:CDS |
| TGGGAAGTTCGGCGTCGATG+AGG | 0.608955 | 7:+72416141 | None:intergenic |
| CGATGATGAAGACGACGACG+AGG | 0.653015 | 7:-72416054 | MsG0780040045.01.T01:CDS |
| GTTGGATTCTTGGTCTACAG+TGG | 0.654343 | 7:+72416163 | None:intergenic |
| ACTGGAGTGCACATTTCTGG+TGG | 0.678942 | 7:-72415783 | MsG0780040045.01.T01:CDS |
| GATAGCGATGATGATTCTGA+CGG | 0.698753 | 7:-72415906 | MsG0780040045.01.T01:CDS |
| GAATTCCGATGAAGAGCCTG+TGG | 0.723860 | 7:-72416027 | MsG0780040045.01.T01:CDS |
| TTCTTCCGTCTAGGACTCGG+CGG | 0.732545 | 7:-72415818 | MsG0780040045.01.T01:CDS |
| GTCGGAAGAAGAATTGACGG+TGG | 0.750092 | 7:+72416118 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGATTGAAGAAGAAGATG+AGG | - | Chr7:72416087-72416106 | MsG0780040045.01.T01:CDS | 30.0% | |
| ! | TTTTTCTGATGATCCACTTG+CGG | - | Chr7:72416183-72416202 | MsG0780040045.01.T01:CDS | 35.0% |
| GATAGCGATGATGATTCTGA+CGG | - | Chr7:72416136-72416155 | MsG0780040045.01.T01:CDS | 40.0% | |
| TGATTCTGACGGCAATGTTT+CGG | - | Chr7:72416147-72416166 | MsG0780040045.01.T01:CDS | 40.0% | |
| TGGATAACATTCTTCCGTCT+AGG | - | Chr7:72416215-72416234 | MsG0780040045.01.T01:CDS | 40.0% | |
| !! | GTGATGGGTTTCTTCTTGAT+CGG | + | Chr7:72415825-72415844 | None:intergenic | 40.0% |
| !! | TGATGGGTTTCTTCTTGATC+GGG | + | Chr7:72415824-72415843 | None:intergenic | 40.0% |
| !! | TTCTTCGTGGTTGTTGGTTT+CGG | + | Chr7:72415777-72415796 | None:intergenic | 40.0% |
| !!! | GGTTTTGTTAGGAAGGTGAT+GGG | + | Chr7:72415840-72415859 | None:intergenic | 40.0% |
| !!! | GTCATCGTCAGTGTTGTTTT+CGG | + | Chr7:72415969-72415988 | None:intergenic | 40.0% |
| !!! | GTTATCTTGGGGGTTTTGTT+AGG | + | Chr7:72415851-72415870 | None:intergenic | 40.0% |
| !!! | TCATCGTCAGTGTTGTTTTC+GGG | + | Chr7:72415968-72415987 | None:intergenic | 40.0% |
| !!! | TTGTTTTCGGGTTCGTTGTT+GGG | + | Chr7:72415956-72415975 | None:intergenic | 40.0% |
| AGGGATTATGCGCGATGATA+AGG | - | Chr7:72416054-72416073 | MsG0780040045.01.T01:CDS | 45.0% | |
| ATGCGCGATGATAAGGGAAA+AGG | - | Chr7:72416061-72416080 | MsG0780040045.01.T01:CDS | 45.0% | |
| GAAGAAGAATTGACGGTGGT+GGG | + | Chr7:72415923-72415942 | None:intergenic | 45.0% | |
| GATTCTGACGGCAATGTTTC+GGG | - | Chr7:72416148-72416167 | MsG0780040045.01.T01:CDS | 45.0% | |
| GATTCTTGGTCTACAGTGGT+GGG | + | Chr7:72415878-72415897 | None:intergenic | 45.0% | |
| GGGATTATGCGCGATGATAA+GGG | - | Chr7:72416055-72416074 | MsG0780040045.01.T01:CDS | 45.0% | |
| GTTGGATTCTTGGTCTACAG+TGG | + | Chr7:72415882-72415901 | None:intergenic | 45.0% | |
| TGCGCGATGATAAGGGAAAA+GGG | - | Chr7:72416062-72416081 | MsG0780040045.01.T01:CDS | 45.0% | |
| TGGTGGGAGTAGTTATCTTG+GGG | + | Chr7:72415862-72415881 | None:intergenic | 45.0% | |
| TTCTTGATCGGGTTTGCGTT+TGG | + | Chr7:72415813-72415832 | None:intergenic | 45.0% | |
| ! | ACATTCTTCCGTCTAGGACT+CGG | - | Chr7:72416221-72416240 | MsG0780040045.01.T01:CDS | 45.0% |
| ! | AGTGGTGGGAGTAGTTATCT+TGG | + | Chr7:72415864-72415883 | None:intergenic | 45.0% |
| ! | GTGGTGGGAGTAGTTATCTT+GGG | + | Chr7:72415863-72415882 | None:intergenic | 45.0% |
| !! | TCTTCGTGGTTGTTGGTTTC+GGG | + | Chr7:72415776-72415795 | None:intergenic | 45.0% |
| !!! | GGGTTTTGTTAGGAAGGTGA+TGG | + | Chr7:72415841-72415860 | None:intergenic | 45.0% |
| !!! | GTTGTTTTCGGGTTCGTTGT+TGG | + | Chr7:72415957-72415976 | None:intergenic | 45.0% |
| !!! | TCTTGGGGGTTTTGTTAGGA+AGG | + | Chr7:72415847-72415866 | None:intergenic | 45.0% |
| CAGACTGGAGTGCACATTTC+TGG | - | Chr7:72416256-72416275 | MsG0780040045.01.T01:CDS | 50.0% | |
| CGTCGATGAGGTTGGATTCT+TGG | + | Chr7:72415892-72415911 | None:intergenic | 50.0% | |
| GAATTCCGATGAAGAGCCTG+TGG | - | Chr7:72416015-72416034 | MsG0780040045.01.T01:CDS | 50.0% | |
| GGAAGAAGAATTGACGGTGG+TGG | + | Chr7:72415924-72415943 | None:intergenic | 50.0% | |
| GGATTCTTGGTCTACAGTGG+TGG | + | Chr7:72415879-72415898 | None:intergenic | 50.0% | |
| GGTGGGAGTAGTTATCTTGG+GGG | + | Chr7:72415861-72415880 | None:intergenic | 50.0% | |
| GTCGGAAGAAGAATTGACGG+TGG | + | Chr7:72415927-72415946 | None:intergenic | 50.0% | |
| TCCAAATCAACCTCCGCAAG+TGG | + | Chr7:72416199-72416218 | None:intergenic | 50.0% | |
| TGATCGGGTTTGCGTTTGGT+GGG | + | Chr7:72415809-72415828 | None:intergenic | 50.0% | |
| TGTGGGTTCTTCGTGGTTGT+TGG | + | Chr7:72415783-72415802 | None:intergenic | 50.0% | |
| TTCTGATGATCCACTTGCGG+AGG | - | Chr7:72416186-72416205 | MsG0780040045.01.T01:CDS | 50.0% | |
| TTGATCGGGTTTGCGTTTGG+TGG | + | Chr7:72415810-72415829 | None:intergenic | 50.0% | |
| ! | ACTGGAGTGCACATTTCTGG+TGG | - | Chr7:72416259-72416278 | MsG0780040045.01.T01:CDS | 50.0% |
| ! | GGCGTCGGAAGAAGAATTGA+CGG | + | Chr7:72415930-72415949 | None:intergenic | 50.0% |
| ! | TCCACTTGCGGAGGTTGATT+TGG | - | Chr7:72416195-72416214 | MsG0780040045.01.T01:CDS | 50.0% |
| ! | TGGTGGACAGGAAGGGAAAA+GGG | - | Chr7:72416035-72416054 | MsG0780040045.01.T01:CDS | 50.0% |
| ! | TTCGGGTTCGTTGTTGGGAA+CGG | + | Chr7:72415951-72415970 | None:intergenic | 50.0% |
| ! | TTGCGTTTGGTGGGGAATGT+GGG | + | Chr7:72415800-72415819 | None:intergenic | 50.0% |
| ! | TTTGCGTTTGGTGGGGAATG+TGG | + | Chr7:72415801-72415820 | None:intergenic | 50.0% |
| !! | ATTGACGGTGGTGGGAAGTT+CGG | + | Chr7:72415915-72415934 | None:intergenic | 50.0% |
| CGATGATGAAGACGACGACG+AGG | - | Chr7:72415988-72416007 | MsG0780040045.01.T01:CDS | 55.0% | |
| GTCCACCACAGGCTCTTCAT+CGG | + | Chr7:72416023-72416042 | None:intergenic | 55.0% | |
| TGGGGAATGTGGGTTCTTCG+TGG | + | Chr7:72415790-72415809 | None:intergenic | 55.0% | |
| TTCCGATGAAGAGCCTGTGG+TGG | - | Chr7:72416018-72416037 | MsG0780040045.01.T01:CDS | 55.0% | |
| TTCGTTGTTGGGAACGGCGT+CGG | + | Chr7:72415945-72415964 | None:intergenic | 55.0% | |
| TTTCCCTTCCTGTCCACCAC+AGG | + | Chr7:72416034-72416053 | None:intergenic | 55.0% | |
| ! | GATCGGGTTTGCGTTTGGTG+GGG | + | Chr7:72415808-72415827 | None:intergenic | 55.0% |
| ! | TTCTTCCGTCTAGGACTCGG+CGG | - | Chr7:72416224-72416243 | MsG0780040045.01.T01:CDS | 55.0% |
| !! | AAGTTCGGCGTCGATGAGGT+TGG | + | Chr7:72415900-72415919 | None:intergenic | 55.0% |
| !! | ATGAAGAGCCTGTGGTGGAC+AGG | - | Chr7:72416023-72416042 | MsG0780040045.01.T01:CDS | 55.0% |
| !! | GTGGTGGACAGGAAGGGAAA+AGG | - | Chr7:72416034-72416053 | MsG0780040045.01.T01:CDS | 55.0% |
| !! | AGAGCCTGTGGTGGACAGGA+AGG | - | Chr7:72416027-72416046 | MsG0780040045.01.T01:CDS | 60.0% |
| !! | GAGCCTGTGGTGGACAGGAA+GGG | - | Chr7:72416028-72416047 | MsG0780040045.01.T01:CDS | 60.0% |
| !! | TGGGAAGTTCGGCGTCGATG+AGG | + | Chr7:72415904-72415923 | None:intergenic | 60.0% |
| GTTCGCCGCCGAGTCCTAGA+CGG | + | Chr7:72416232-72416251 | None:intergenic | 65.0% | |
| CGGCGGCGAACTGCTCAGAC+TGG | - | Chr7:72416241-72416260 | MsG0780040045.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 72415764 | 72416300 | 72415764 | ID=MsG0780040045.01;Name=MsG0780040045.01 |
| Chr7 | mRNA | 72415764 | 72416300 | 72415764 | ID=MsG0780040045.01.T01;Parent=MsG0780040045.01;Name=MsG0780040045.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|178 |
| Chr7 | exon | 72415764 | 72416300 | 72415764 | ID=MsG0780040045.01.T01:exon:9842;Parent=MsG0780040045.01.T01 |
| Chr7 | CDS | 72415764 | 72416300 | 72415764 | ID=MsG0780040045.01.T01:cds;Parent=MsG0780040045.01.T01 |
| Gene Sequence |
| Protein sequence |
