Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017788.01.T01 | XP_013462141.1 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 9.79E-136 | 387 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017788.01.T01 | A4QND5 | 61.497 | 187 | 64 | 1 | 1 | 187 | 1 | 179 | 1.01E-79 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017788.01.T01 | I3ST48 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 4.68e-136 | 387 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048428.01 | MsG0380017788.01 | 0.817893 | 2.603031e-52 | 1.919450e-49 |
| MsG0180002023.01 | MsG0380017788.01 | 0.861062 | 1.335377e-63 | 3.729600e-60 |
| MsG0180002458.01 | MsG0380017788.01 | 0.826772 | 2.257927e-54 | 2.137747e-51 |
| MsG0180003117.01 | MsG0380017788.01 | 0.852327 | 4.965309e-61 | 1.033904e-57 |
| MsG0180004129.01 | MsG0380017788.01 | 0.815700 | 8.081808e-52 | 5.612313e-49 |
| MsG0180004130.01 | MsG0380017788.01 | 0.820118 | 8.122657e-53 | 6.371226e-50 |
| MsG0180004656.01 | MsG0380017788.01 | 0.839509 | 1.518259e-57 | 2.101312e-54 |
| MsG0180004915.01 | MsG0380017788.01 | 0.860426 | 2.082716e-63 | 5.691027e-60 |
| MsG0180005851.01 | MsG0380017788.01 | 0.826812 | 2.208491e-54 | 2.093471e-51 |
| MsG0180005957.01 | MsG0380017788.01 | 0.841521 | 4.519696e-58 | 6.658360e-55 |
| MsG0280006327.01 | MsG0380017788.01 | 0.863856 | 1.846373e-64 | 5.679500e-61 |
| MsG0280006406.01 | MsG0380017788.01 | 0.839703 | 1.352468e-57 | 1.882805e-54 |
| MsG0280011387.01 | MsG0380017788.01 | 0.811426 | 7.041814e-51 | 4.357710e-48 |
| MsG0380012108.01 | MsG0380017788.01 | 0.864407 | 1.243043e-64 | 3.898736e-61 |
| MsG0380012566.01 | MsG0380017788.01 | 0.825905 | 3.632638e-54 | 3.355628e-51 |
| MsG0380014577.01 | MsG0380017788.01 | 0.858281 | 9.173124e-63 | 2.328952e-59 |
| MsG0380017788.01 | MsG0480021904.01 | 0.850381 | 1.762018e-60 | 3.444971e-57 |
| MsG0380017788.01 | MsG0480023388.01 | 0.811100 | 8.288305e-51 | 5.085028e-48 |
| MsG0380017788.01 | MsG0480023968.01 | 0.860664 | 1.764008e-63 | 4.859868e-60 |
| MsG0380017788.01 | MsG0580024974.01 | 0.840409 | 8.850926e-58 | 1.259675e-54 |
| MsG0380017788.01 | MsG0580025400.01 | 0.849770 | 2.612922e-60 | 5.007135e-57 |
| MsG0380017788.01 | MsG0580025421.01 | 0.840700 | 7.423951e-58 | 1.066141e-54 |
| MsG0380017788.01 | MsG0580027043.01 | 0.811889 | 5.586659e-51 | 3.499454e-48 |
| MsG0380017788.01 | MsG0580028958.01 | 0.854002 | 1.645102e-61 | 3.621868e-58 |
| MsG0380017788.01 | MsG0680030285.01 | 0.835206 | 1.919979e-56 | 2.331708e-53 |
| MsG0380017788.01 | MsG0680030414.01 | 0.887682 | 1.151039e-72 | 8.646687e-69 |
| MsG0380017788.01 | MsG0680031822.01 | 0.811055 | 8.475450e-51 | 5.193896e-48 |
| MsG0380017788.01 | MsG0680035854.01 | 0.800081 | 1.703541e-48 | 7.860621e-46 |
| MsG0380017788.01 | MsG0780036813.01 | 0.815156 | 1.067864e-51 | 7.305390e-49 |
| MsG0380017788.01 | MsG0780039289.01 | 0.849859 | 2.466487e-60 | 4.740661e-57 |
| MsG0380017788.01 | MsG0780040045.01 | 0.800768 | 1.234575e-48 | 5.796579e-46 |
| MsG0380017788.01 | MsG0780040338.01 | 0.804866 | 1.758291e-49 | 9.167464e-47 |
| MsG0380017788.01 | MsG0780041519.01 | 0.806175 | 9.345053e-50 | 5.038539e-47 |
| MsG0380017788.01 | MsG0780041688.01 | 0.844964 | 5.453451e-59 | 8.951711e-56 |
| MsG0380017788.01 | MsG0880046321.01 | 0.834481 | 2.923530e-56 | 3.474031e-53 |
| MsG0380017788.01 | MsG0880046328.01 | 0.829186 | 5.925401e-55 | 6.020375e-52 |
| MsG0380017788.01 | MsG0880046449.01 | 0.821603 | 3.698704e-53 | 3.022906e-50 |
| MsG0380017788.01 | MsG0880046575.01 | 0.877181 | 7.720846e-69 | 3.846096e-65 |
| MsG0380017788.01 | MsG0880047286.01 | 0.851391 | 9.151877e-61 | 1.849670e-57 |
| MsG0380017788.01 | MsG0880047448.01 | 0.815627 | 8.390020e-52 | 5.815019e-49 |
| MsG0280007690.01 | MsG0380017788.01 | 0.844501 | 7.271728e-59 | 1.176309e-55 |
| MsG0280008283.01 | MsG0380017788.01 | 0.801540 | 8.580859e-49 | 4.108428e-46 |
| MsG0280009230.01 | MsG0380017788.01 | 0.863477 | 2.421165e-64 | 7.348936e-61 |
| MsG0280009375.01 | MsG0380017788.01 | 0.808077 | 3.697238e-50 | 2.093987e-47 |
| MsG0280009716.01 | MsG0380017788.01 | 0.855600 | 5.657440e-62 | 1.313172e-58 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017788.01.T01 | MTR_3g112090 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 1.19e-139 | 387 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017788.01.T01 | AT4G15770 | 83.422 | 187 | 31 | 0 | 1 | 187 | 1 | 187 | 3.92e-119 | 335 |
Find 46 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGATGTGCCTTTGGGTTT+TGG | 0.195441 | 3:+100662298 | MsG0380017788.01.T01:CDS |
| TTCTATGTCGGATGTGCCTT+TGG | 0.274575 | 3:+100662291 | MsG0380017788.01.T01:CDS |
| TGGTATTGTTGTGCTTCATC+AGG | 0.357825 | 3:+100662366 | MsG0380017788.01.T01:CDS |
| GTGCTTCATCAGGGTGATAT+TGG | 0.380581 | 3:+100662376 | MsG0380017788.01.T01:CDS |
| GAGTGCGTTGGGTCGGATTA+CGG | 0.395512 | 3:+100662234 | MsG0380017788.01.T01:CDS |
| TCTATGTCGGATGTGCCTTT+GGG | 0.403032 | 3:+100662292 | MsG0380017788.01.T01:CDS |
| CTAAGTCTACACAGGATTGT+AGG | 0.417131 | 3:+100662329 | MsG0380017788.01.T01:CDS |
| GTGATATTGGAGAGTATTTG+AGG | 0.418524 | 3:+100662389 | MsG0380017788.01.T01:CDS |
| CTCGTTTCTGTCTCGTCCAA+AGG | 0.450814 | 3:-100661872 | None:intergenic |
| TGGAGAGTATTTGAGGATTG+AGG | 0.451876 | 3:+100662396 | MsG0380017788.01.T01:CDS |
| ATTACGGAGAATATTGTTGC+TGG | 0.453018 | 3:+100662250 | MsG0380017788.01.T01:CDS |
| AGAATATTGTTGCTGGTGAT+GGG | 0.475550 | 3:+100662257 | MsG0380017788.01.T01:CDS |
| GAGAATATTGTTGCTGGTGA+TGG | 0.477429 | 3:+100662256 | MsG0380017788.01.T01:CDS |
| TGAAGCACAACAATACCATT+AGG | 0.481187 | 3:-100662361 | None:intergenic |
| GTTCCGAGGGAAACGAGGTT+AGG | 0.488759 | 3:-100662070 | None:intergenic |
| TACACAGGATTGTAGGAAGT+TGG | 0.488772 | 3:+100662336 | MsG0380017788.01.T01:CDS |
| GTAGCGACCAGGATTTGAAT+CGG | 0.501537 | 3:-100661972 | None:intergenic |
| GTTTCCCTCGGAACGTGTAT+CGG | 0.505430 | 3:+100662079 | MsG0380017788.01.T01:CDS |
| TGTAGGAAGTTGGATCCTAA+TGG | 0.511689 | 3:+100662346 | MsG0380017788.01.T01:CDS |
| TTTAAGCCATACCTTATGCT+TGG | 0.513488 | 3:-100662167 | None:intergenic |
| TGCTGCGAATGCCAAGCATA+AGG | 0.516412 | 3:+100662156 | MsG0380017788.01.T01:CDS |
| GAAGGTCCCGATTCAAATCC+TGG | 0.525646 | 3:+100661965 | MsG0380017788.01.T01:CDS |
| GAATCGGGACCTTCGTGTGA+GGG | 0.536023 | 3:-100661956 | None:intergenic |
| AGACCTAACCTCGTTTCCCT+CGG | 0.540105 | 3:+100662067 | MsG0380017788.01.T01:CDS |
| GAGGTTAGGTCTAGCGATGT+TGG | 0.552615 | 3:-100662056 | None:intergenic |
| CATGTGTTGAAGAGTGCGTT+GGG | 0.555269 | 3:+100662223 | MsG0380017788.01.T01:CDS |
| TAGCGACCAGGATTTGAATC+GGG | 0.568635 | 3:-100661971 | None:intergenic |
| GAAAAGCTCTTCAAATTCGT+AGG | 0.571649 | 3:+100661905 | MsG0380017788.01.T01:CDS |
| CGAATGCCAAGCATAAGGTA+TGG | 0.574051 | 3:+100662161 | MsG0380017788.01.T01:CDS |
| TCATGTGTTGAAGAGTGCGT+TGG | 0.587350 | 3:+100662222 | MsG0380017788.01.T01:CDS |
| AGACGGAAACAGTAGCGACC+AGG | 0.591481 | 3:-100661983 | None:intergenic |
| GGTATTGTTGTGCTTCATCA+GGG | 0.595232 | 3:+100662367 | MsG0380017788.01.T01:CDS |
| TGTTGGTGGCACGCTTAACG+AGG | 0.595506 | 3:-100662039 | None:intergenic |
| GTTAGGTCTAGCGATGTTGG+TGG | 0.599334 | 3:-100662053 | None:intergenic |
| TGTTGAAGAGTGCGTTGGGT+CGG | 0.603803 | 3:+100662227 | MsG0380017788.01.T01:CDS |
| TACACGTTCCGAGGGAAACG+AGG | 0.615959 | 3:-100662075 | None:intergenic |
| TGAATCGGGACCTTCGTGTG+AGG | 0.619897 | 3:-100661957 | None:intergenic |
| AAGTAAATTCAACGCCTGAA+CGG | 0.628881 | 3:-100662134 | None:intergenic |
| GAATATTGTTGCTGGTGATG+GGG | 0.643723 | 3:+100662258 | MsG0380017788.01.T01:CDS |
| GTTGGTGGCACGCTTAACGA+GGG | 0.651920 | 3:-100662038 | None:intergenic |
| GGTGGCTGCTAAGTCTACAC+AGG | 0.655794 | 3:+100662321 | MsG0380017788.01.T01:CDS |
| GCAGCCACCCCAAAACCCAA+AGG | 0.658414 | 3:-100662307 | None:intergenic |
| GTTGAAAATCCCTCACACGA+AGG | 0.698320 | 3:+100661947 | MsG0380017788.01.T01:CDS |
| GGACGAGACAGAAACGAGCG+TGG | 0.721090 | 3:+100661877 | MsG0380017788.01.T01:CDS |
| TGTATCGGAAAATACACACA+CGG | 0.738380 | 3:+100662094 | MsG0380017788.01.T01:CDS |
| ATCGGAAAATACACACACGG+TGG | 0.745885 | 3:+100662097 | MsG0380017788.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAAGACATCTCAGATTG+TGG | - | Chr3:100662193-100662212 | None:intergenic | 30.0% | |
| ! | AGATTTTGTTCTTGTTGAGA+CGG | - | Chr3:100662003-100662022 | None:intergenic | 30.0% |
| !!! | CTGAGATGTCTTTTTTGTAT+GGG | + | Chr3:100662197-100662216 | MsG0380017788.01.T01:CDS | 30.0% |
| !!! | GGTTGTTGTTTTTTCTATGT+CGG | + | Chr3:100662279-100662298 | MsG0380017788.01.T01:CDS | 30.0% |
| !!! | TCTGAGATGTCTTTTTTGTA+TGG | + | Chr3:100662196-100662215 | MsG0380017788.01.T01:CDS | 30.0% |
| AAGTAAATTCAACGCCTGAA+CGG | - | Chr3:100662137-100662156 | None:intergenic | 35.0% | |
| AGAATATTGTTGCTGGTGAT+GGG | + | Chr3:100662257-100662276 | MsG0380017788.01.T01:CDS | 35.0% | |
| ATTACGGAGAATATTGTTGC+TGG | + | Chr3:100662250-100662269 | MsG0380017788.01.T01:CDS | 35.0% | |
| GAAAAGCTCTTCAAATTCGT+AGG | + | Chr3:100661905-100661924 | MsG0380017788.01.T01:CDS | 35.0% | |
| TGAAGCACAACAATACCATT+AGG | - | Chr3:100662364-100662383 | None:intergenic | 35.0% | |
| TGTATCGGAAAATACACACA+CGG | + | Chr3:100662094-100662113 | MsG0380017788.01.T01:CDS | 35.0% | |
| TTTAAGCCATACCTTATGCT+TGG | - | Chr3:100662170-100662189 | None:intergenic | 35.0% | |
| ! | AAATTTTCATCTCACCGTTC+AGG | + | Chr3:100662120-100662139 | MsG0380017788.01.T01:CDS | 35.0% |
| !! | GTGATATTGGAGAGTATTTG+AGG | + | Chr3:100662389-100662408 | MsG0380017788.01.T01:CDS | 35.0% |
| CTAAGTCTACACAGGATTGT+AGG | + | Chr3:100662329-100662348 | MsG0380017788.01.T01:CDS | 40.0% | |
| GAGAATATTGTTGCTGGTGA+TGG | + | Chr3:100662256-100662275 | MsG0380017788.01.T01:CDS | 40.0% | |
| GGTATTGTTGTGCTTCATCA+GGG | + | Chr3:100662367-100662386 | MsG0380017788.01.T01:CDS | 40.0% | |
| TACACAGGATTGTAGGAAGT+TGG | + | Chr3:100662336-100662355 | MsG0380017788.01.T01:CDS | 40.0% | |
| TGGTATTGTTGTGCTTCATC+AGG | + | Chr3:100662366-100662385 | MsG0380017788.01.T01:CDS | 40.0% | |
| TGTAGGAAGTTGGATCCTAA+TGG | + | Chr3:100662346-100662365 | MsG0380017788.01.T01:CDS | 40.0% | |
| ! | GAATATTGTTGCTGGTGATG+GGG | + | Chr3:100662258-100662277 | MsG0380017788.01.T01:CDS | 40.0% |
| !! | TGGAGAGTATTTGAGGATTG+AGG | + | Chr3:100662396-100662415 | MsG0380017788.01.T01:CDS | 40.0% |
| ATCGGAAAATACACACACGG+TGG | + | Chr3:100662097-100662116 | MsG0380017788.01.T01:CDS | 45.0% | |
| CGAATGCCAAGCATAAGGTA+TGG | + | Chr3:100662161-100662180 | MsG0380017788.01.T01:CDS | 45.0% | |
| GTAGCGACCAGGATTTGAAT+CGG | - | Chr3:100661975-100661994 | None:intergenic | 45.0% | |
| GTGCTTCATCAGGGTGATAT+TGG | + | Chr3:100662376-100662395 | MsG0380017788.01.T01:CDS | 45.0% | |
| GTTGAAAATCCCTCACACGA+AGG | + | Chr3:100661947-100661966 | MsG0380017788.01.T01:CDS | 45.0% | |
| TAGCGACCAGGATTTGAATC+GGG | - | Chr3:100661974-100661993 | None:intergenic | 45.0% | |
| TCTATGTCGGATGTGCCTTT+GGG | + | Chr3:100662292-100662311 | MsG0380017788.01.T01:CDS | 45.0% | |
| TTCTATGTCGGATGTGCCTT+TGG | + | Chr3:100662291-100662310 | MsG0380017788.01.T01:CDS | 45.0% | |
| ! | ATTTTCCGATACACGTTCCG+AGG | - | Chr3:100662087-100662106 | None:intergenic | 45.0% |
| ! | CATGTGTTGAAGAGTGCGTT+GGG | + | Chr3:100662223-100662242 | MsG0380017788.01.T01:CDS | 45.0% |
| ! | TCATGTGTTGAAGAGTGCGT+TGG | + | Chr3:100662222-100662241 | MsG0380017788.01.T01:CDS | 45.0% |
| ! | TTTTCCGATACACGTTCCGA+GGG | - | Chr3:100662086-100662105 | None:intergenic | 45.0% |
| AGACCTAACCTCGTTTCCCT+CGG | + | Chr3:100662067-100662086 | MsG0380017788.01.T01:CDS | 50.0% | |
| CTCGTTTCTGTCTCGTCCAA+AGG | - | Chr3:100661875-100661894 | None:intergenic | 50.0% | |
| GAAGGTCCCGATTCAAATCC+TGG | + | Chr3:100661965-100661984 | MsG0380017788.01.T01:CDS | 50.0% | |
| GAGGTTAGGTCTAGCGATGT+TGG | - | Chr3:100662059-100662078 | None:intergenic | 50.0% | |
| GTTAGGTCTAGCGATGTTGG+TGG | - | Chr3:100662056-100662075 | None:intergenic | 50.0% | |
| GTTTCCCTCGGAACGTGTAT+CGG | + | Chr3:100662079-100662098 | MsG0380017788.01.T01:CDS | 50.0% | |
| TGCTGCGAATGCCAAGCATA+AGG | + | Chr3:100662156-100662175 | MsG0380017788.01.T01:CDS | 50.0% | |
| ! | CGTGTGAGGGATTTTCAACG+AGG | - | Chr3:100661946-100661965 | None:intergenic | 50.0% |
| ! | TGTTGAAGAGTGCGTTGGGT+CGG | + | Chr3:100662227-100662246 | MsG0380017788.01.T01:CDS | 50.0% |
| !! | CGGATGTGCCTTTGGGTTTT+GGG | + | Chr3:100662299-100662318 | MsG0380017788.01.T01:CDS | 50.0% |
| !! | GGATGTGCCTTTGGGTTTTG+GGG | + | Chr3:100662300-100662319 | MsG0380017788.01.T01:CDS | 50.0% |
| !! | TCGGATGTGCCTTTGGGTTT+TGG | + | Chr3:100662298-100662317 | MsG0380017788.01.T01:CDS | 50.0% |
| AGACGGAAACAGTAGCGACC+AGG | - | Chr3:100661986-100662005 | None:intergenic | 55.0% | |
| GAATCGGGACCTTCGTGTGA+GGG | - | Chr3:100661959-100661978 | None:intergenic | 55.0% | |
| GAGTGCGTTGGGTCGGATTA+CGG | + | Chr3:100662234-100662253 | MsG0380017788.01.T01:CDS | 55.0% | |
| GGTGGCTGCTAAGTCTACAC+AGG | + | Chr3:100662321-100662340 | MsG0380017788.01.T01:CDS | 55.0% | |
| GTTCCGAGGGAAACGAGGTT+AGG | - | Chr3:100662073-100662092 | None:intergenic | 55.0% | |
| TACACGTTCCGAGGGAAACG+AGG | - | Chr3:100662078-100662097 | None:intergenic | 55.0% | |
| TGAATCGGGACCTTCGTGTG+AGG | - | Chr3:100661960-100661979 | None:intergenic | 55.0% | |
| !! | GTTGGTGGCACGCTTAACGA+GGG | - | Chr3:100662041-100662060 | None:intergenic | 55.0% |
| !! | TGTTGGTGGCACGCTTAACG+AGG | - | Chr3:100662042-100662061 | None:intergenic | 55.0% |
| !!! | TGTGCCTTTGGGTTTTGGGG+TGG | + | Chr3:100662303-100662322 | MsG0380017788.01.T01:CDS | 55.0% |
| GCAGCCACCCCAAAACCCAA+AGG | - | Chr3:100662310-100662329 | None:intergenic | 60.0% | |
| GGACGAGACAGAAACGAGCG+TGG | + | Chr3:100661877-100661896 | MsG0380017788.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 100661866 | 100662429 | 100661866 | ID=MsG0380017788.01;Name=MsG0380017788.01 |
| Chr3 | mRNA | 100661866 | 100662429 | 100661866 | ID=MsG0380017788.01.T01;Parent=MsG0380017788.01;Name=MsG0380017788.01.T01;_AED=0.22;_eAED=0.22;_QI=0|-1|0|1|-1|1|1|0|187 |
| Chr3 | exon | 100661866 | 100662429 | 100661866 | ID=MsG0380017788.01.T01:exon:2358;Parent=MsG0380017788.01.T01 |
| Chr3 | CDS | 100661866 | 100662429 | 100661866 | ID=MsG0380017788.01.T01:cds;Parent=MsG0380017788.01.T01 |
| Gene Sequence |
| Protein sequence |
