AlfalfaGEDB | MsG0780040890.01

M. sativa cultivar ZhongmuNo.1 / MsG0780040890.01

KEGG: -
GO: GO:0003700 GO:0006355 GO:0043565
PFAM: PF03106

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040890.01.T01	XP_003625561.1	75.385	390	34	3	1	346	1	372	0	571
MsG0780040890.01.T01	XP_003625561.1	60.714	56	21	1	146	200	362	417	1.08E-11	77.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040890.01.T01	Q9ZUU0	34.456	386	186	11	2	346	1	360	1.02E-50	177

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048849.01	MsG0780040890.01	0.805609	1.229270e-49	6.531975e-47
MsG0180000599.01	MsG0780040890.01	0.824160	9.382980e-54	8.245637e-51
MsG0180000782.01	MsG0780040890.01	0.810473	1.132863e-50	6.835165e-48
MsG0180000814.01	MsG0780040890.01	0.814146	1.787472e-51	1.189913e-48
MsG0180001100.01	MsG0780040890.01	0.804415	2.183918e-49	1.125346e-46
MsG0180002024.01	MsG0780040890.01	0.813345	2.682344e-51	1.747319e-48
MsG0180002399.01	MsG0780040890.01	0.804180	2.444892e-49	1.252250e-46
MsG0180004246.01	MsG0780040890.01	0.832441	9.435469e-56	1.054746e-52
MsG0180004765.01	MsG0780040890.01	0.806508	7.949440e-50	4.322477e-47
MsG0280006914.01	MsG0780040890.01	0.825468	4.611123e-54	4.206285e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040890.01.T01	MTR_7g100510	75.385	390	34	3	1	346	1	372	0.0	571
MsG0780040890.01.T01	MTR_7g100510	60.714	56	21	1	146	200	362	417	1.31e-15	77.8
MsG0780040890.01.T01	MTR_5g042920	33.432	338	143	12	26	346	59	331	2.36e-43	155
MsG0780040890.01.T01	MTR_5g042920	64.286	56	19	1	146	200	321	376	1.57e-16	80.5
MsG0780040890.01.T01	MTR_7g011820	41.892	222	111	4	130	346	213	421	3.46e-42	154
MsG0780040890.01.T01	MTR_7g011820	60.714	56	21	1	146	200	411	466	1.66e-14	74.7
MsG0780040890.01.T01	MTR_6g046600	94.118	68	4	0	161	228	18	85	4.32e-40	137
MsG0780040890.01.T01	MTR_7g080020	39.370	254	108	8	127	346	263	504	1.71e-37	143
MsG0780040890.01.T01	MTR_7g080020	58.929	56	22	1	146	200	494	549	5.26e-13	70.5
MsG0780040890.01.T01	MTR_5g094430	37.143	245	119	6	107	346	27	241	5.18e-37	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780040890.01.T01	AT2G37260	34.456	386	186	11	2	346	1	360	1.04e-51	177
MsG0780040890.01.T01	AT2G37260	58.929	56	22	1	146	200	350	405	3.06e-14	73.9
MsG0780040890.01.T01	AT2G03340	39.423	208	101	2	139	346	244	426	4.83e-41	150
MsG0780040890.01.T01	AT2G03340	62.500	56	20	1	146	200	416	471	1.38e-16	81.3
MsG0780040890.01.T01	AT1G13960	38.991	218	103	5	139	346	70	267	2.88e-39	142
MsG0780040890.01.T01	AT1G13960	62.500	56	20	1	146	200	257	312	2.72e-16	79.3
MsG0780040890.01.T01	AT1G13960	40.271	221	96	6	139	346	223	420	1.12e-38	144
MsG0780040890.01.T01	AT1G13960	62.500	56	20	1	146	200	410	465	1.92e-16	80.9
MsG0780040890.01.T01	AT2G37260	41.228	228	92	7	137	346	75	278	2.46e-38	140
MsG0780040890.01.T01	AT2G37260	58.929	56	22	1	146	200	268	323	3.71e-14	73.2

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Find 82 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTCTTGTGGTCTAAGT+GGG	0.545127	7:-83897813	MsG0780040890.01.T01:CDS
AAACTACAATATCCGCCATT+AGG	0.452468	7:-83899461	MsG0780040890.01.T01:CDS
AAAGTTGAAAGATCATTAGA+TGG	0.489403	7:-83898042	MsG0780040890.01.T01:CDS
AAATGAAGTGGCAGTATCAG+AGG	0.580061	7:-83897208	MsG0780040890.01.T01:CDS
AACGAATATCTCCCCTTCTC+AGG	0.464851	7:-83899412	MsG0780040890.01.T01:intron
AAGCAAAGGATTTGAGGCAG+AGG	0.578305	7:-83897777	MsG0780040890.01.T01:CDS
AATATGGGCAGAGGTGGTGA+AGG	0.476994	7:-83897107	None:intergenic
AATCAGAATGAAAGGAATGA+AGG	0.486904	7:-83897886	MsG0780040890.01.T01:CDS
ACAACATCAAAAGCGAAACT+CGG	0.546405	7:-83897971	MsG0780040890.01.T01:CDS
ACATCAGGAATGATTTCTGA+TGG	0.574578	7:-83897943	MsG0780040890.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTCTTGTGGTCTAAGT+GGG	-	Chr7:83898894-83898913	MsG0780040890.01.T01:intron	30.0%
AATCAGAATGAAAGGAATGA+AGG	-	Chr7:83898821-83898840	MsG0780040890.01.T01:intron	30.0%
ACGAAAAAGATTACAAACGA+AGG	+	Chr7:83897822-83897841	None:intergenic	30.0%
AGAAAGGTTATTCTTCATAG+TGG	-	Chr7:83897872-83897891	MsG0780040890.01.T01:CDS	30.0%
AGTCACTAACACAAATATAG+CGG	+	Chr7:83898230-83898249	None:intergenic	30.0%
ATCTCTCATTTACAAACCAA+TGG	-	Chr7:83897650-83897669	MsG0780040890.01.T01:intron	30.0%
CTCCAAAAGAAGATAAAAAG+AGG	+	Chr7:83898312-83898331	None:intergenic	30.0%
GAATGGTTCAAGATAAAGTA+TGG	-	Chr7:83898786-83898805	MsG0780040890.01.T01:intron	30.0%
GATTTGTATGAAGAACAAGT+AGG	+	Chr7:83897170-83897189	None:intergenic	30.0%
GGATATAACTGGAGAAAATA+TGG	-	Chr7:83898566-83898585	MsG0780040890.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	83897110	83899619	83897110	ID=MsG0780040890.01;Name=MsG0780040890.01
Chr7	mRNA	83897110	83899619	83897110	ID=MsG0780040890.01.T01;Parent=MsG0780040890.01;Name=MsG0780040890.01.T01;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|347
Chr7	exon	83899413	83899619	83899413	ID=MsG0780040890.01.T01:exon:11466;Parent=MsG0780040890.01.T01
Chr7	exon	83897762	83898394	83897762	ID=MsG0780040890.01.T01:exon:11465;Parent=MsG0780040890.01.T01
Chr7	exon	83897392	83897456	83897392	ID=MsG0780040890.01.T01:exon:11464;Parent=MsG0780040890.01.T01
Chr7	exon	83897110	83897248	83897110	ID=MsG0780040890.01.T01:exon:11463;Parent=MsG0780040890.01.T01
Chr7	CDS	83899413	83899619	83899413	ID=MsG0780040890.01.T01:cds;Parent=MsG0780040890.01.T01
Chr7	CDS	83897762	83898394	83897762	ID=MsG0780040890.01.T01:cds;Parent=MsG0780040890.01.T01
Chr7	CDS	83897392	83897456	83897392	ID=MsG0780040890.01.T01:cds;Parent=MsG0780040890.01.T01
Chr7	CDS	83897110	83897248	83897110	ID=MsG0780040890.01.T01:cds;Parent=MsG0780040890.01.T01

Gene Sequence

>MsG0780040890.01.T01

ATGATGGATATTGAAGAAGCAGAAAGAGTAGTTGTTGCCAAACCAGTTGCTTCAAGGCCTACTTGTTCTTCATACAAATCTTTCTCAGAGCTTCTAGCAGGCGCAATCAATTCCACTCCTCCAATTGCATCTTCTCAAACTACAATATCCGCCATTAGGCCAAAGACAGTGAGGTTCAAGCCAGCAACGAATATCTCCCCTTCTCAGGGAATTTGCAGTACTAATCAGCCACAACCACAGAAATCAATGGAGACCAATCCCCAGCATCTTAACCATGACAACTTTAGAGCTAACACGAGCTCGAAATTACATCAGAACATTACCCTTCCTACAGAAACATATCAGGCCACAGAATCTTGTACGATGGCACCACAGAACATGGAGGAGGACCAAAAAGCATTACCATCCACAAATAATGCTGATAGACCTTCGTATGATGGATATAACTGGAGAAAATATGGACAGAAGCAAGTAAAAGGAAGCGAGTATCCTCGAAGTTACTACAAGTGCACATATCCTAATTGTCCAGTTAAAAAGAAAGTTGAAAGATCATTAGATGGTGAGATTGCAGAAATTGTTTATAAAGGTGAACACAACCATGGAAAGCAACAACATCAAAAGCGAAACTCGGGAGCAACATCAGGAATGATTTCTGATGGAATGGTTCAAGATAAAGTATGGAGTAATAATAGTAATCAGAATGAAAGGAATGAAGGAAGAATAGATAATCAAGTCAAAGCTTCACAGCCTAATGATTTTGCTCTAGAAAATTCTTGTGGTCTAAGTGGGAATGTGAGGAAGGAAGCAAAGGATTTGAGGCAGAGGAAGATGACCCTAGAATTCAACCATGATTTGGATGTTTCATTGATTCAATCTCTTGTCCGATTTTTAATTTTAATTCATTGGGAAAATGAGAATCAATCAAATGAAGTGGCAGTATCAGAGGAAGGTTTAGTAGAGCCAGGCATTGTAATGCAAAGTTCTGTGGATTCTGAAGTCCTTGGAGATGGCTTTCGCTGGAGGAAATATGGGCAGAGGTGGTGA

Protein sequence

>MsG0780040890.01.T01

MMDIEEAERVVVAKPVASRPTCSSYKSFSELLAGAINSTPPIASSQTTISAIRPKTVRFKPATNISPSQGICSTNQPQPQKSMETNPQHLNHDNFRANTSSKLHQNITLPTETYQATESCTMAPQNMEEDQKALPSTNNADRPSYDGYNWRKYGQKQVKGSEYPRSYYKCTYPNCPVKKKVERSLDGEIAEIVYKGEHNHGKQQHQKRNSGATSGMISDGMVQDKVWSNNSNQNERNEGRIDNQVKASQPNDFALENSCGLSGNVRKEAKDLRQRKMTLEFNHDLDVSLIQSLVRFLILIHWENENQSNEVAVSEEGLVEPGIVMQSSVDSEVLGDGFRWRKYGQRW*