Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041612.01.T01 | XP_003626244.1 | 98.077 | 156 | 3 | 0 | 1 | 156 | 1 | 156 | 1.72E-109 | 325 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041612.01.T01 | I6YGS0 | 39.241 | 158 | 93 | 3 | 1 | 156 | 1 | 157 | 1.54E-35 | 129 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041612.01.T01 | A2Q321 | 98.077 | 156 | 3 | 0 | 1 | 156 | 1 | 156 | 8.21e-110 | 325 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048506.01 | MsG0780041612.01 | 0.811580 | 6.521197e-51 | 4.051759e-48 |
| MsG0080048507.01 | MsG0780041612.01 | 0.822099 | 2.839738e-53 | 2.353792e-50 |
| MsG0180003992.01 | MsG0780041612.01 | 0.810783 | 9.706938e-51 | 5.905570e-48 |
| MsG0780041510.01 | MsG0780041612.01 | 0.931684 | 2.622163e-94 | 1.681081e-89 |
| MsG0280011372.01 | MsG0780041612.01 | -0.803135 | 4.028528e-49 | 2.008666e-46 |
| MsG0680032167.01 | MsG0780041612.01 | 0.831805 | 1.355432e-55 | 1.487154e-52 |
| MsG0580024178.01 | MsG0780041612.01 | 0.830681 | 2.560950e-55 | 2.717542e-52 |
| MsG0380015369.01 | MsG0780041612.01 | 0.800993 | 1.110273e-48 | 5.243569e-46 |
| MsG0380017610.01 | MsG0780041612.01 | 0.823062 | 1.695721e-53 | 1.444723e-50 |
| MsG0380017637.01 | MsG0780041612.01 | 0.827187 | 1.796575e-54 | 1.721852e-51 |
| MsG0780036881.01 | MsG0780041612.01 | -0.806493 | 8.010799e-50 | 4.354097e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041612.01.T01 | MTR_7g113030 | 98.077 | 156 | 3 | 0 | 1 | 156 | 1 | 156 | 2.08e-113 | 325 |
| MsG0780041612.01.T01 | MTR_7g113050 | 94.231 | 156 | 9 | 0 | 1 | 156 | 1 | 156 | 5.68e-110 | 313 |
| MsG0780041612.01.T01 | MTR_7g113050 | 94.231 | 156 | 9 | 0 | 1 | 156 | 1 | 156 | 3.05e-109 | 315 |
| MsG0780041612.01.T01 | MTR_7g112615 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 6.52e-107 | 305 |
| MsG0780041612.01.T01 | MTR_7g112615 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 8.67e-107 | 303 |
| MsG0780041612.01.T01 | MTR_7g112615 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 3.19e-106 | 307 |
| MsG0780041612.01.T01 | MTR_7g112970 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 3.54e-101 | 294 |
| MsG0780041612.01.T01 | MTR_7g112960 | 78.977 | 176 | 34 | 1 | 1 | 176 | 1 | 173 | 5.30e-101 | 293 |
| MsG0780041612.01.T01 | MTR_7g112620 | 87.179 | 156 | 20 | 0 | 1 | 156 | 1 | 156 | 7.52e-101 | 293 |
| MsG0780041612.01.T01 | MTR_7g113020 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 2.24e-100 | 292 |
| MsG0780041612.01.T01 | MTR_7g112963 | 86.538 | 156 | 21 | 0 | 1 | 156 | 1 | 156 | 3.01e-100 | 291 |
| MsG0780041612.01.T01 | MTR_7g113010 | 84.615 | 156 | 24 | 0 | 1 | 156 | 1 | 156 | 1.71e-97 | 293 |
| MsG0780041612.01.T01 | MTR_1g083480 | 72.472 | 178 | 37 | 3 | 1 | 175 | 1 | 169 | 1.04e-91 | 265 |
| MsG0780041612.01.T01 | MTR_4g005160 | 77.987 | 159 | 31 | 2 | 1 | 156 | 1 | 158 | 1.37e-89 | 265 |
| MsG0780041612.01.T01 | MTR_4g120240 | 66.456 | 158 | 50 | 2 | 1 | 156 | 37 | 193 | 8.15e-73 | 223 |
| MsG0780041612.01.T01 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 2.29e-71 | 215 |
| MsG0780041612.01.T01 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 2.49e-70 | 216 |
| MsG0780041612.01.T01 | MTR_3g024250 | 78.862 | 123 | 22 | 2 | 46 | 168 | 1 | 119 | 3.18e-66 | 199 |
| MsG0780041612.01.T01 | MTR_8g076680 | 53.846 | 156 | 71 | 1 | 1 | 156 | 3 | 157 | 1.32e-61 | 191 |
| MsG0780041612.01.T01 | MTR_8g076680 | 53.846 | 156 | 71 | 1 | 1 | 156 | 3 | 157 | 7.71e-61 | 191 |
| MsG0780041612.01.T01 | MTR_4g089060 | 41.975 | 162 | 88 | 2 | 1 | 161 | 1 | 157 | 7.63e-41 | 140 |
| MsG0780041612.01.T01 | MTR_4g089060 | 41.026 | 156 | 87 | 1 | 1 | 156 | 1 | 151 | 1.51e-40 | 137 |
| MsG0780041612.01.T01 | MTR_4g089060 | 41.026 | 156 | 87 | 1 | 1 | 156 | 1 | 151 | 4.80e-40 | 137 |
| MsG0780041612.01.T01 | MTR_4g089040 | 41.026 | 156 | 87 | 1 | 1 | 156 | 1 | 151 | 6.60e-38 | 132 |
| MsG0780041612.01.T01 | MTR_2g090240 | 39.103 | 156 | 91 | 1 | 1 | 156 | 1 | 152 | 1.25e-36 | 129 |
| MsG0780041612.01.T01 | MTR_2g090245 | 38.462 | 156 | 92 | 2 | 1 | 156 | 1 | 152 | 2.36e-35 | 125 |
| MsG0780041612.01.T01 | MTR_7g112910 | 51.765 | 85 | 25 | 1 | 72 | 156 | 1 | 69 | 6.32e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041612.01.T01 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 2.87e-81 | 241 |
| MsG0780041612.01.T01 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 1.90e-80 | 242 |
| MsG0780041612.01.T01 | AT4G02340 | 64.744 | 156 | 54 | 1 | 1 | 156 | 1 | 155 | 8.82e-74 | 224 |
| MsG0780041612.01.T01 | AT2G26740 | 64.151 | 159 | 51 | 3 | 4 | 156 | 1 | 159 | 5.94e-69 | 212 |
| MsG0780041612.01.T01 | AT4G15955 | 61.728 | 162 | 52 | 2 | 5 | 156 | 6 | 167 | 1.04e-68 | 207 |
| MsG0780041612.01.T01 | AT4G15955 | 61.728 | 162 | 52 | 2 | 5 | 156 | 6 | 167 | 1.12e-68 | 207 |
| MsG0780041612.01.T01 | AT4G15955 | 60.479 | 167 | 56 | 2 | 5 | 161 | 6 | 172 | 1.13e-67 | 208 |
| MsG0780041612.01.T01 | AT2G26750 | 60.000 | 160 | 59 | 3 | 4 | 158 | 1 | 160 | 1.37e-65 | 204 |
| MsG0780041612.01.T01 | AT4G15960 | 57.407 | 162 | 63 | 2 | 1 | 156 | 51 | 212 | 2.62e-64 | 202 |
| MsG0780041612.01.T01 | AT3G51000 | 55.844 | 154 | 66 | 2 | 4 | 156 | 5 | 157 | 7.44e-58 | 184 |
Find 45 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATAAGCTTTGATTCTTTC+AGG | 0.126719 | 7:-93013238 | None:intergenic |
| GGGATTGCTTAAAGTAAATT+AGG | 0.138380 | 7:-93013409 | None:intergenic |
| GGCATCAACGGTCCTGATTT+TGG | 0.162298 | 7:-93013293 | None:intergenic |
| CCTCTTGTCTTGTTCCTTCA+TGG | 0.187509 | 7:+93012968 | MsG0780041612.01.T01:CDS |
| TTATTACATATGTAGATTTC+AGG | 0.264776 | 7:+93013339 | MsG0780041612.01.T01:CDS |
| AAAATGCATATTGCTGAAAA+AGG | 0.266560 | 7:+93012935 | MsG0780041612.01.T01:CDS |
| GGATTGCTTAAAGTAAATTA+GGG | 0.321616 | 7:-93013408 | None:intergenic |
| CGCCATGAATACCACAGTTC+GGG | 0.324950 | 7:-93012995 | None:intergenic |
| CACAGAACAGTGGAAGTAAA+TGG | 0.328559 | 7:+93012908 | MsG0780041612.01.T01:CDS |
| ATTTCTTGTGGCTCATGATT+GGG | 0.336317 | 7:+93013180 | MsG0780041612.01.T01:CDS |
| TATTTCTTGTGGCTCATGAT+TGG | 0.353373 | 7:+93013179 | MsG0780041612.01.T01:CDS |
| CATGATTGGGGTGCCATCAT+TGG | 0.372270 | 7:+93013193 | MsG0780041612.01.T01:CDS |
| GATGCCATGCATGCTGCTTA+TGG | 0.379998 | 7:+93013310 | MsG0780041612.01.T01:CDS |
| GCTCCGGATCTCCGTGGTTA+TGG | 0.399328 | 7:+93013061 | MsG0780041612.01.T01:CDS |
| TCCCCGAACTGTGGTATTCA+TGG | 0.400391 | 7:+93012993 | MsG0780041612.01.T01:CDS |
| CATAAGCTTTGATTCTTTCA+GGG | 0.418162 | 7:-93013237 | None:intergenic |
| TCCGTAGAAACCCCAAAATC+AGG | 0.443771 | 7:+93013281 | MsG0780041612.01.T01:CDS |
| CGGCTACCGTGCTGTTGCTC+CGG | 0.456232 | 7:+93013045 | MsG0780041612.01.T01:CDS |
| GAAAATCTATAAGTGCAATA+AGG | 0.461083 | 7:-93013141 | None:intergenic |
| GCGCCATGAATACCACAGTT+CGG | 0.461173 | 7:-93012996 | None:intergenic |
| TCACCATAACCACGGAGATC+CGG | 0.490155 | 7:-93013064 | None:intergenic |
| CAGAGACAAAAGTAGAAAAG+TGG | 0.532538 | 7:-93013381 | None:intergenic |
| ACAAAGATACCAACCAATGA+TGG | 0.534758 | 7:-93013206 | None:intergenic |
| TTTCTTGTGGCTCATGATTG+GGG | 0.543062 | 7:+93013181 | MsG0780041612.01.T01:CDS |
| TTGGGGTTTCTACGGAGTAG+TGG | 0.547389 | 7:-93013274 | None:intergenic |
| ATTGTAGCTCTCAGCTCCCT+CGG | 0.548619 | 7:+93013025 | MsG0780041612.01.T01:CDS |
| ATCTCCATAAGCAGCATGCA+TGG | 0.554133 | 7:-93013314 | None:intergenic |
| GAGCAACAGCACGGTAGCCG+AGG | 0.563671 | 7:-93013042 | None:intergenic |
| AGCAACAGCACGGTAGCCGA+GGG | 0.572130 | 7:-93013041 | None:intergenic |
| ATTGGGGTGCCATCATTGGT+TGG | 0.574625 | 7:+93013197 | MsG0780041612.01.T01:CDS |
| ACAGTTCGGGGAAGCCATGA+AGG | 0.577839 | 7:-93012982 | None:intergenic |
| AGCTGAGAGCTACAATCTGA+TGG | 0.590990 | 7:-93013018 | None:intergenic |
| AGCAGCATGCATGGCATCAA+CGG | 0.592010 | 7:-93013305 | None:intergenic |
| TCTTCAGTTAGTAGCTACAC+AGG | 0.594878 | 7:+93013100 | MsG0780041612.01.T01:CDS |
| TTCATGGCTTCCCCGAACTG+TGG | 0.605850 | 7:+93012984 | MsG0780041612.01.T01:CDS |
| TGTTGATCAAGTATTTCTTG+TGG | 0.608957 | 7:+93013168 | MsG0780041612.01.T01:CDS |
| ACTAACTGAAGACGGAGCAT+CGG | 0.615174 | 7:-93013089 | None:intergenic |
| CCATGAAGGAACAAGACAAG+AGG | 0.618716 | 7:-93012968 | None:intergenic |
| GTGTAGCTACTAACTGAAGA+CGG | 0.652352 | 7:-93013097 | None:intergenic |
| GATTGCTTAAAGTAAATTAG+GGG | 0.664444 | 7:-93013407 | None:intergenic |
| GGAGATCCGGAGCAACAGCA+CGG | 0.668358 | 7:-93013051 | None:intergenic |
| AGGAATAGAACACAGAACAG+TGG | 0.676997 | 7:+93012898 | MsG0780041612.01.T01:CDS |
| GCCATGAATACCACAGTTCG+GGG | 0.692309 | 7:-93012994 | None:intergenic |
| GCTGTTGCTCCGGATCTCCG+TGG | 0.713696 | 7:+93013055 | MsG0780041612.01.T01:CDS |
| CATCGGTGTCACCATAACCA+CGG | 0.714613 | 7:-93013072 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTACATATGTAGATTTC+AGG | + | Chr7:93013339-93013358 | MsG0780041612.01.T01:CDS | 20.0% |
| ! | AAAATGCATATTGCTGAAAA+AGG | + | Chr7:93012935-93012954 | MsG0780041612.01.T01:CDS | 25.0% |
| ! | GAAAATCTATAAGTGCAATA+AGG | - | Chr7:93013144-93013163 | None:intergenic | 25.0% |
| !! | ATTGCACTTATAGATTTTCT+GGG | + | Chr7:93013145-93013164 | MsG0780041612.01.T01:CDS | 25.0% |
| !! | TATTGCACTTATAGATTTTC+TGG | + | Chr7:93013144-93013163 | MsG0780041612.01.T01:CDS | 25.0% |
| ATTGCTGAAAAAGGAAAAGA+AGG | + | Chr7:93012944-93012963 | MsG0780041612.01.T01:CDS | 30.0% | |
| TGTTGATCAAGTATTTCTTG+TGG | + | Chr7:93013168-93013187 | MsG0780041612.01.T01:CDS | 30.0% | |
| ! | ACATAAGCTTTGATTCTTTC+AGG | - | Chr7:93013241-93013260 | None:intergenic | 30.0% |
| ! | CATAAGCTTTGATTCTTTCA+GGG | - | Chr7:93013240-93013259 | None:intergenic | 30.0% |
| ! | TACACAGGTTTTCATATAGT+AGG | + | Chr7:93013115-93013134 | MsG0780041612.01.T01:CDS | 30.0% |
| ACAAAGATACCAACCAATGA+TGG | - | Chr7:93013209-93013228 | None:intergenic | 35.0% | |
| ATTTCTTGTGGCTCATGATT+GGG | + | Chr7:93013180-93013199 | MsG0780041612.01.T01:CDS | 35.0% | |
| CAGAGACAAAAGTAGAAAAG+TGG | - | Chr7:93013384-93013403 | None:intergenic | 35.0% | |
| TATTTCTTGTGGCTCATGAT+TGG | + | Chr7:93013179-93013198 | MsG0780041612.01.T01:CDS | 35.0% | |
| AGGAATAGAACACAGAACAG+TGG | + | Chr7:93012898-93012917 | MsG0780041612.01.T01:CDS | 40.0% | |
| CACAGAACAGTGGAAGTAAA+TGG | + | Chr7:93012908-93012927 | MsG0780041612.01.T01:CDS | 40.0% | |
| GTGTAGCTACTAACTGAAGA+CGG | - | Chr7:93013100-93013119 | None:intergenic | 40.0% | |
| TCTTCAGTTAGTAGCTACAC+AGG | + | Chr7:93013100-93013119 | MsG0780041612.01.T01:CDS | 40.0% | |
| TTTCTTGTGGCTCATGATTG+GGG | + | Chr7:93013181-93013200 | MsG0780041612.01.T01:CDS | 40.0% | |
| !!! | TCCTGATTTTGGGGTTTCTA+CGG | - | Chr7:93013285-93013304 | None:intergenic | 40.0% |
| ACTAACTGAAGACGGAGCAT+CGG | - | Chr7:93013092-93013111 | None:intergenic | 45.0% | |
| AGCTGAGAGCTACAATCTGA+TGG | - | Chr7:93013021-93013040 | None:intergenic | 45.0% | |
| ATCTCCATAAGCAGCATGCA+TGG | - | Chr7:93013317-93013336 | None:intergenic | 45.0% | |
| CCATGAAGGAACAAGACAAG+AGG | - | Chr7:93012971-93012990 | None:intergenic | 45.0% | |
| TCCGTAGAAACCCCAAAATC+AGG | + | Chr7:93013281-93013300 | MsG0780041612.01.T01:CDS | 45.0% | |
| ! | CATCAACGGTCCTGATTTTG+GGG | - | Chr7:93013294-93013313 | None:intergenic | 45.0% |
| ! | CCTCTTGTCTTGTTCCTTCA+TGG | + | Chr7:93012968-93012987 | MsG0780041612.01.T01:CDS | 45.0% |
| ! | GCATCAACGGTCCTGATTTT+GGG | - | Chr7:93013295-93013314 | None:intergenic | 45.0% |
| AGCAGCATGCATGGCATCAA+CGG | - | Chr7:93013308-93013327 | None:intergenic | 50.0% | |
| ATTGTAGCTCTCAGCTCCCT+CGG | + | Chr7:93013025-93013044 | MsG0780041612.01.T01:CDS | 50.0% | |
| CGCCATGAATACCACAGTTC+GGG | - | Chr7:93012998-93013017 | None:intergenic | 50.0% | |
| GCCATGAATACCACAGTTCG+GGG | - | Chr7:93012997-93013016 | None:intergenic | 50.0% | |
| GCGCCATGAATACCACAGTT+CGG | - | Chr7:93012999-93013018 | None:intergenic | 50.0% | |
| TCACCATAACCACGGAGATC+CGG | - | Chr7:93013067-93013086 | None:intergenic | 50.0% | |
| TCCCCGAACTGTGGTATTCA+TGG | + | Chr7:93012993-93013012 | MsG0780041612.01.T01:CDS | 50.0% | |
| TTGGGGTTTCTACGGAGTAG+TGG | - | Chr7:93013277-93013296 | None:intergenic | 50.0% | |
| ! | ATTGGGGTGCCATCATTGGT+TGG | + | Chr7:93013197-93013216 | MsG0780041612.01.T01:CDS | 50.0% |
| ! | CATGATTGGGGTGCCATCAT+TGG | + | Chr7:93013193-93013212 | MsG0780041612.01.T01:CDS | 50.0% |
| ! | GATGCCATGCATGCTGCTTA+TGG | + | Chr7:93013310-93013329 | MsG0780041612.01.T01:CDS | 50.0% |
| ! | GGCATCAACGGTCCTGATTT+TGG | - | Chr7:93013296-93013315 | None:intergenic | 50.0% |
| !! | CATCGGTGTCACCATAACCA+CGG | - | Chr7:93013075-93013094 | None:intergenic | 50.0% |
| ACAGTTCGGGGAAGCCATGA+AGG | - | Chr7:93012985-93013004 | None:intergenic | 55.0% | |
| TTCATGGCTTCCCCGAACTG+TGG | + | Chr7:93012984-93013003 | MsG0780041612.01.T01:CDS | 55.0% | |
| AGCAACAGCACGGTAGCCGA+GGG | - | Chr7:93013044-93013063 | None:intergenic | 60.0% | |
| GCTCCGGATCTCCGTGGTTA+TGG | + | Chr7:93013061-93013080 | MsG0780041612.01.T01:CDS | 60.0% | |
| GGAGATCCGGAGCAACAGCA+CGG | - | Chr7:93013054-93013073 | None:intergenic | 60.0% | |
| GAGCAACAGCACGGTAGCCG+AGG | - | Chr7:93013045-93013064 | None:intergenic | 65.0% | |
| GCTGTTGCTCCGGATCTCCG+TGG | + | Chr7:93013055-93013074 | MsG0780041612.01.T01:CDS | 65.0% | |
| ! | CGGCTACCGTGCTGTTGCTC+CGG | + | Chr7:93013045-93013064 | MsG0780041612.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93012893 | 93013423 | 93012893 | ID=MsG0780041612.01;Name=MsG0780041612.01 |
| Chr7 | mRNA | 93012893 | 93013423 | 93012893 | ID=MsG0780041612.01.T01;Parent=MsG0780041612.01;Name=MsG0780041612.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|176 |
| Chr7 | exon | 93012893 | 93013423 | 93012893 | ID=MsG0780041612.01.T01:exon:40804;Parent=MsG0780041612.01.T01 |
| Chr7 | CDS | 93012893 | 93013423 | 93012893 | ID=MsG0780041612.01.T01:cds;Parent=MsG0780041612.01.T01 |
| Gene Sequence |
| Protein sequence |
