Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041652.01.T01 | XP_013450528.1 | 93.689 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 9.15E-132 | 378 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041652.01.T01 | P02877 | 69.951 | 203 | 54 | 3 | 6 | 204 | 1 | 200 | 1.05E-102 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041652.01.T01 | A0A072U5A4 | 93.689 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 4.37e-132 | 378 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000337.01 | MsG0780041652.01 | 0.830844 | 2.335832e-55 | 2.490618e-52 |
| MsG0280011338.01 | MsG0780041652.01 | 0.818884 | 1.552637e-52 | 1.176664e-49 |
| MsG0480023107.01 | MsG0780041652.01 | 0.805418 | 1.348120e-49 | 7.128487e-47 |
| MsG0280007912.01 | MsG0780041652.01 | 0.813889 | 2.036788e-51 | 1.346494e-48 |
| MsG0280010020.01 | MsG0780041652.01 | 0.800814 | 1.207780e-48 | 5.677468e-46 |
| MsG0280010054.01 | MsG0780041652.01 | 0.813631 | 2.321498e-51 | 1.524000e-48 |
| MsG0280010055.01 | MsG0780041652.01 | 0.828299 | 9.709491e-55 | 9.611549e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041652.01.T01 | MTR_7g115220 | 93.689 | 206 | 9 | 1 | 1 | 206 | 1 | 202 | 1.11e-135 | 378 |
| MsG0780041652.01.T01 | MTR_1g080820 | 71.901 | 121 | 34 | 0 | 68 | 188 | 24 | 144 | 6.18e-64 | 194 |
| MsG0780041652.01.T01 | MTR_1g080810 | 71.074 | 121 | 35 | 0 | 68 | 188 | 24 | 144 | 2.56e-63 | 193 |
| MsG0780041652.01.T01 | MTR_1g080780 | 70.000 | 120 | 36 | 0 | 71 | 190 | 22 | 141 | 3.59e-63 | 192 |
| MsG0780041652.01.T01 | MTR_1g080800 | 70.732 | 123 | 36 | 0 | 68 | 190 | 24 | 146 | 1.09e-62 | 191 |
| MsG0780041652.01.T01 | MTR_6g083270 | 50.000 | 154 | 60 | 2 | 61 | 197 | 15 | 168 | 1.54e-43 | 151 |
| MsG0780041652.01.T01 | MTR_6g083200 | 47.692 | 130 | 64 | 2 | 61 | 188 | 15 | 142 | 2.92e-38 | 131 |
| MsG0780041652.01.T01 | MTR_6g083290 | 47.445 | 137 | 61 | 2 | 61 | 190 | 15 | 147 | 1.86e-34 | 124 |
| MsG0780041652.01.T01 | MTR_6g083310 | 42.667 | 150 | 69 | 3 | 66 | 200 | 20 | 167 | 1.30e-33 | 118 |
| MsG0780041652.01.T01 | MTR_3g118390 | 50.000 | 66 | 33 | 0 | 1 | 66 | 59 | 124 | 3.63e-16 | 76.3 |
| MsG0780041652.01.T01 | MTR_8g074335 | 65.854 | 41 | 14 | 0 | 22 | 62 | 22 | 62 | 3.08e-12 | 64.7 |
| MsG0780041652.01.T01 | MTR_8g074350 | 48.214 | 56 | 29 | 0 | 6 | 61 | 7 | 62 | 3.86e-11 | 61.6 |
| MsG0780041652.01.T01 | MTR_8g074330 | 65.000 | 40 | 14 | 0 | 22 | 61 | 22 | 61 | 6.43e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041652.01.T01 | AT3G04720 | 70.813 | 209 | 53 | 3 | 3 | 203 | 2 | 210 | 2.11e-96 | 279 |
| MsG0780041652.01.T01 | AT3G12500 | 65.854 | 41 | 13 | 1 | 22 | 61 | 33 | 73 | 3.74e-11 | 61.6 |
Find 59 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATTAGACATGGGAAAATT+TGG | 0.218092 | 7:+93486319 | None:intergenic |
| ATGATGCAGGTGACAAATTC+AGG | 0.293677 | 7:+93486909 | MsG0780041652.01.T01:intron |
| CAGCAATGGAGGGTTGGATT+TGG | 0.326401 | 7:+93486971 | MsG0780041652.01.T01:CDS |
| TACTCATGGCGTAGCAAGTA+TGG | 0.353266 | 7:+93486643 | MsG0780041652.01.T01:CDS |
| CTTCTTGTGGAAAGTGTCTC+AGG | 0.353594 | 7:+93486707 | MsG0780041652.01.T01:CDS |
| TGGCTGCAACAAAGGTTGTT+TGG | 0.365069 | 7:-93486430 | None:intergenic |
| GCTTGACCACGAGGTCCAAC+AGG | 0.397549 | 7:-93486685 | None:intergenic |
| GGAGGGTTGGATTTGGATGT+AGG | 0.409363 | 7:+93486978 | MsG0780041652.01.T01:CDS |
| AAGTCCCATCCATGTTGCTC+TGG | 0.422201 | 7:-93486574 | None:intergenic |
| TATTGTAAGCTATCAGTTTG+TGG | 0.428774 | 7:+93487052 | MsG0780041652.01.T01:CDS |
| GCTATCAGTTTGTGGATTGT+GGG | 0.462963 | 7:+93487060 | MsG0780041652.01.T01:CDS |
| AGATGGCAGAGGATACCAAC+AGG | 0.473028 | 7:+93487022 | MsG0780041652.01.T01:CDS |
| AACCTTTGTTGCAGCCAATA+CGG | 0.476377 | 7:+93486436 | MsG0780041652.01.T01:CDS |
| TACTTGCTACGCCATGAGTA+AGG | 0.479661 | 7:-93486640 | None:intergenic |
| AACCGTATTGGCTGCAACAA+AGG | 0.489204 | 7:-93486438 | None:intergenic |
| AAATTCAGGGACGGGAGCAC+AGG | 0.490592 | 7:+93486923 | MsG0780041652.01.T01:CDS |
| AGCTATCAGTTTGTGGATTG+TGG | 0.492406 | 7:+93487059 | MsG0780041652.01.T01:CDS |
| CGGTACCACACCAACCGTAT+TGG | 0.497937 | 7:-93486450 | None:intergenic |
| TTATTGTTTAGCATCAATGA+TGG | 0.506297 | 7:-93487114 | None:intergenic |
| GAACAATGCGGTAAACAAGC+TGG | 0.511319 | 7:+93486394 | MsG0780041652.01.T01:CDS |
| TAAGTGCTTATTGTTCAACT+TGG | 0.529463 | 7:+93486605 | MsG0780041652.01.T01:CDS |
| TGACAATTACTCTGACAGTT+CGG | 0.538086 | 7:-93486490 | None:intergenic |
| CAGGTGACAAATTCAGGGAC+GGG | 0.545443 | 7:+93486915 | MsG0780041652.01.T01:intron |
| CCTCGTGGTCAAGCTTCTTG+TGG | 0.556286 | 7:+93486694 | MsG0780041652.01.T01:CDS |
| TCAATGCAGCAATGGAGGGT+TGG | 0.558488 | 7:+93486965 | MsG0780041652.01.T01:CDS |
| AAGTGCTTATTGTTCAACTT+GGG | 0.564174 | 7:+93486606 | MsG0780041652.01.T01:CDS |
| AATGATGGAGAACAGAGGAT+TGG | 0.564526 | 7:-93487099 | None:intergenic |
| GTAGATCAATGCAGCAATGG+AGG | 0.578522 | 7:+93486960 | MsG0780041652.01.T01:CDS |
| TTTGTTGCAGCCAATACGGT+TGG | 0.578744 | 7:+93486440 | MsG0780041652.01.T01:CDS |
| TGATGCAGGTGACAAATTCA+GGG | 0.583955 | 7:+93486910 | MsG0780041652.01.T01:intron |
| ATGCAAGCAAACCTTACTCA+TGG | 0.586811 | 7:+93486629 | MsG0780041652.01.T01:CDS |
| ATCAACCAGAGCAACATGGA+TGG | 0.587569 | 7:+93486569 | MsG0780041652.01.T01:CDS |
| TCAACCAGAGCAACATGGAT+GGG | 0.598724 | 7:+93486570 | MsG0780041652.01.T01:CDS |
| CATGGCGTAGCAAGTATGGT+TGG | 0.600676 | 7:+93486647 | MsG0780041652.01.T01:CDS |
| TAGATCAATGCAGCAATGGA+GGG | 0.603290 | 7:+93486961 | MsG0780041652.01.T01:CDS |
| TTACAATAAGATGTCCCTGT+TGG | 0.604159 | 7:-93487037 | None:intergenic |
| GCAGGTGACAAATTCAGGGA+CGG | 0.605105 | 7:+93486914 | MsG0780041652.01.T01:intron |
| AGTAATTGTCATGGCAGTAG+TGG | 0.614410 | 7:+93486502 | MsG0780041652.01.T01:CDS |
| TGTGGCCCTGTTGGACCTCG+TGG | 0.621769 | 7:+93486679 | MsG0780041652.01.T01:CDS |
| TGTCATGGCAGTAGTGGTGG+TGG | 0.621989 | 7:+93486508 | MsG0780041652.01.T01:CDS |
| TTCTTGTGGAAAGTGTCTCA+GGG | 0.622227 | 7:+93486708 | MsG0780041652.01.T01:CDS |
| AATTGTCATGGCAGTAGTGG+TGG | 0.624496 | 7:+93486505 | MsG0780041652.01.T01:CDS |
| AAGCCTAGCGGTATTAACAT+TGG | 0.627981 | 7:+93486345 | MsG0780041652.01.T01:CDS |
| AACTGTCAGAGTAATTGTCA+TGG | 0.629228 | 7:+93486493 | MsG0780041652.01.T01:CDS |
| ATTGTAGATCAATGCAGCAA+TGG | 0.636888 | 7:+93486957 | MsG0780041652.01.T01:CDS |
| TACTATCAACCAGAGCAACA+TGG | 0.648851 | 7:+93486565 | MsG0780041652.01.T01:CDS |
| TGCAGCCAATACGGTTGGTG+TGG | 0.651269 | 7:+93486445 | MsG0780041652.01.T01:CDS |
| TTTAACAGAATCGACACAGA+TGG | 0.651956 | 7:+93487005 | MsG0780041652.01.T01:CDS |
| CCACAAGAAGCTTGACCACG+AGG | 0.656609 | 7:-93486694 | None:intergenic |
| AGAATCGACACAGATGGCAG+AGG | 0.657142 | 7:+93487011 | MsG0780041652.01.T01:CDS |
| ACACCAATGTTAATACCGCT+AGG | 0.661884 | 7:-93486348 | None:intergenic |
| CAATGCGGTAAACAAGCTGG+AGG | 0.695826 | 7:+93486397 | MsG0780041652.01.T01:CDS |
| CTTGACCACGAGGTCCAACA+GGG | 0.697195 | 7:-93486684 | None:intergenic |
| GCATCAATGATGGAGAACAG+AGG | 0.707012 | 7:-93487104 | None:intergenic |
| CGGACCACAGTATTCATCTG+CGG | 0.727310 | 7:-93486470 | None:intergenic |
| GATGGCAGAGGATACCAACA+GGG | 0.745988 | 7:+93487023 | MsG0780041652.01.T01:CDS |
| ACAACAGTTGCAGAACAATG+CGG | 0.746680 | 7:+93486382 | MsG0780041652.01.T01:CDS |
| AAAATTTGGAGTAAGCCTAG+CGG | 0.781644 | 7:+93486333 | MsG0780041652.01.T01:CDS |
| GGTACCGCAGATGAATACTG+TGG | 0.800059 | 7:+93486466 | MsG0780041652.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGAGTAAATATAGATAAAA+AGG | - | Chr7:93486765-93486784 | None:intergenic | 15.0% |
| !!! | TACTCTAATGTTTTAAAAAT+AGG | + | Chr7:93486778-93486797 | MsG0780041652.01.T01:intron | 15.0% |
| !! | TAGATAAAAAGGAATAGAAT+TGG | - | Chr7:93486754-93486773 | None:intergenic | 20.0% |
| ! | TTATTGTTTAGCATCAATGA+TGG | - | Chr7:93487117-93487136 | None:intergenic | 25.0% |
| AAGTGCTTATTGTTCAACTT+GGG | + | Chr7:93486606-93486625 | MsG0780041652.01.T01:CDS | 30.0% | |
| TAAGTGCTTATTGTTCAACT+TGG | + | Chr7:93486605-93486624 | MsG0780041652.01.T01:CDS | 30.0% | |
| TATTGTAAGCTATCAGTTTG+TGG | + | Chr7:93487052-93487071 | MsG0780041652.01.T01:CDS | 30.0% | |
| AACTGTCAGAGTAATTGTCA+TGG | + | Chr7:93486493-93486512 | MsG0780041652.01.T01:CDS | 35.0% | |
| ATTGTAGATCAATGCAGCAA+TGG | + | Chr7:93486957-93486976 | MsG0780041652.01.T01:CDS | 35.0% | |
| TGACAATTACTCTGACAGTT+CGG | - | Chr7:93486493-93486512 | None:intergenic | 35.0% | |
| TTACAATAAGATGTCCCTGT+TGG | - | Chr7:93487040-93487059 | None:intergenic | 35.0% | |
| TTTAACAGAATCGACACAGA+TGG | + | Chr7:93487005-93487024 | MsG0780041652.01.T01:CDS | 35.0% | |
| ! | AAAATTTGGAGTAAGCCTAG+CGG | + | Chr7:93486333-93486352 | MsG0780041652.01.T01:CDS | 35.0% |
| ! | GAATGTAAATGTGATGATGC+AGG | + | Chr7:93486896-93486915 | MsG0780041652.01.T01:intron | 35.0% |
| AACCTTTGTTGCAGCCAATA+CGG | + | Chr7:93486436-93486455 | MsG0780041652.01.T01:CDS | 40.0% | |
| AAGCCTAGCGGTATTAACAT+TGG | + | Chr7:93486345-93486364 | MsG0780041652.01.T01:CDS | 40.0% | |
| ACAACAGTTGCAGAACAATG+CGG | + | Chr7:93486382-93486401 | MsG0780041652.01.T01:CDS | 40.0% | |
| ACACCAATGTTAATACCGCT+AGG | - | Chr7:93486351-93486370 | None:intergenic | 40.0% | |
| AGTAATTGTCATGGCAGTAG+TGG | + | Chr7:93486502-93486521 | MsG0780041652.01.T01:CDS | 40.0% | |
| ATGATGCAGGTGACAAATTC+AGG | + | Chr7:93486909-93486928 | MsG0780041652.01.T01:intron | 40.0% | |
| ATGCAAGCAAACCTTACTCA+TGG | + | Chr7:93486629-93486648 | MsG0780041652.01.T01:CDS | 40.0% | |
| TACTATCAACCAGAGCAACA+TGG | + | Chr7:93486565-93486584 | MsG0780041652.01.T01:CDS | 40.0% | |
| TAGATCAATGCAGCAATGGA+GGG | + | Chr7:93486961-93486980 | MsG0780041652.01.T01:CDS | 40.0% | |
| TGATGCAGGTGACAAATTCA+GGG | + | Chr7:93486910-93486929 | MsG0780041652.01.T01:intron | 40.0% | |
| ! | AATGATGGAGAACAGAGGAT+TGG | - | Chr7:93487102-93487121 | None:intergenic | 40.0% |
| ! | AGCTATCAGTTTGTGGATTG+TGG | + | Chr7:93487059-93487078 | MsG0780041652.01.T01:CDS | 40.0% |
| ! | GCTATCAGTTTGTGGATTGT+GGG | + | Chr7:93487060-93487079 | MsG0780041652.01.T01:CDS | 40.0% |
| !! | TTCTTGTGGAAAGTGTCTCA+GGG | + | Chr7:93486708-93486727 | MsG0780041652.01.T01:CDS | 40.0% |
| !!! | TATGGTTGGACTGCTTTTTG+TGG | + | Chr7:93486661-93486680 | MsG0780041652.01.T01:CDS | 40.0% |
| AATTGTCATGGCAGTAGTGG+TGG | + | Chr7:93486505-93486524 | MsG0780041652.01.T01:CDS | 45.0% | |
| ATCAACCAGAGCAACATGGA+TGG | + | Chr7:93486569-93486588 | MsG0780041652.01.T01:CDS | 45.0% | |
| GAACAATGCGGTAAACAAGC+TGG | + | Chr7:93486394-93486413 | MsG0780041652.01.T01:CDS | 45.0% | |
| GTAGATCAATGCAGCAATGG+AGG | + | Chr7:93486960-93486979 | MsG0780041652.01.T01:CDS | 45.0% | |
| TACTCATGGCGTAGCAAGTA+TGG | + | Chr7:93486643-93486662 | MsG0780041652.01.T01:CDS | 45.0% | |
| TCAACCAGAGCAACATGGAT+GGG | + | Chr7:93486570-93486589 | MsG0780041652.01.T01:CDS | 45.0% | |
| TGGCTGCAACAAAGGTTGTT+TGG | - | Chr7:93486433-93486452 | None:intergenic | 45.0% | |
| TTTGTTGCAGCCAATACGGT+TGG | + | Chr7:93486440-93486459 | MsG0780041652.01.T01:CDS | 45.0% | |
| ! | GCATCAATGATGGAGAACAG+AGG | - | Chr7:93487107-93487126 | None:intergenic | 45.0% |
| ! | TACTTGCTACGCCATGAGTA+AGG | - | Chr7:93486643-93486662 | None:intergenic | 45.0% |
| !! | AACCGTATTGGCTGCAACAA+AGG | - | Chr7:93486441-93486460 | None:intergenic | 45.0% |
| !! | CTTCTTGTGGAAAGTGTCTC+AGG | + | Chr7:93486707-93486726 | MsG0780041652.01.T01:CDS | 45.0% |
| AAGTCCCATCCATGTTGCTC+TGG | - | Chr7:93486577-93486596 | None:intergenic | 50.0% | |
| AGAATCGACACAGATGGCAG+AGG | + | Chr7:93487011-93487030 | MsG0780041652.01.T01:CDS | 50.0% | |
| AGATGGCAGAGGATACCAAC+AGG | + | Chr7:93487022-93487041 | MsG0780041652.01.T01:CDS | 50.0% | |
| CAATGCGGTAAACAAGCTGG+AGG | + | Chr7:93486397-93486416 | MsG0780041652.01.T01:CDS | 50.0% | |
| CAGCAATGGAGGGTTGGATT+TGG | + | Chr7:93486971-93486990 | MsG0780041652.01.T01:CDS | 50.0% | |
| CAGGTGACAAATTCAGGGAC+GGG | + | Chr7:93486915-93486934 | MsG0780041652.01.T01:intron | 50.0% | |
| CATGGCGTAGCAAGTATGGT+TGG | + | Chr7:93486647-93486666 | MsG0780041652.01.T01:CDS | 50.0% | |
| CGGACCACAGTATTCATCTG+CGG | - | Chr7:93486473-93486492 | None:intergenic | 50.0% | |
| GATGGCAGAGGATACCAACA+GGG | + | Chr7:93487023-93487042 | MsG0780041652.01.T01:CDS | 50.0% | |
| GCAGGTGACAAATTCAGGGA+CGG | + | Chr7:93486914-93486933 | MsG0780041652.01.T01:intron | 50.0% | |
| GGAGGGTTGGATTTGGATGT+AGG | + | Chr7:93486978-93486997 | MsG0780041652.01.T01:CDS | 50.0% | |
| GGTACCGCAGATGAATACTG+TGG | + | Chr7:93486466-93486485 | MsG0780041652.01.T01:CDS | 50.0% | |
| TCAATGCAGCAATGGAGGGT+TGG | + | Chr7:93486965-93486984 | MsG0780041652.01.T01:CDS | 50.0% | |
| !!! | ACTGCTTTTTGTGGCCCTGT+TGG | + | Chr7:93486670-93486689 | MsG0780041652.01.T01:CDS | 50.0% |
| CCACAAGAAGCTTGACCACG+AGG | - | Chr7:93486697-93486716 | None:intergenic | 55.0% | |
| CCTCGTGGTCAAGCTTCTTG+TGG | + | Chr7:93486694-93486713 | MsG0780041652.01.T01:CDS | 55.0% | |
| CGGTACCACACCAACCGTAT+TGG | - | Chr7:93486453-93486472 | None:intergenic | 55.0% | |
| CTTGACCACGAGGTCCAACA+GGG | - | Chr7:93486687-93486706 | None:intergenic | 55.0% | |
| TGTCATGGCAGTAGTGGTGG+TGG | + | Chr7:93486508-93486527 | MsG0780041652.01.T01:CDS | 55.0% | |
| ! | AAATTCAGGGACGGGAGCAC+AGG | + | Chr7:93486923-93486942 | MsG0780041652.01.T01:CDS | 55.0% |
| ! | TGCAGCCAATACGGTTGGTG+TGG | + | Chr7:93486445-93486464 | MsG0780041652.01.T01:CDS | 55.0% |
| GCTTGACCACGAGGTCCAAC+AGG | - | Chr7:93486688-93486707 | None:intergenic | 60.0% | |
| TGTGGCCCTGTTGGACCTCG+TGG | + | Chr7:93486679-93486698 | MsG0780041652.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 93486328 | 93487136 | 93486328 | ID=MsG0780041652.01;Name=MsG0780041652.01 |
| Chr7 | mRNA | 93486328 | 93487136 | 93486328 | ID=MsG0780041652.01.T01;Parent=MsG0780041652.01;Name=MsG0780041652.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.5|2|0|206 |
| Chr7 | exon | 93486328 | 93486729 | 93486328 | ID=MsG0780041652.01.T01:exon:20483;Parent=MsG0780041652.01.T01 |
| Chr7 | exon | 93486918 | 93487136 | 93486918 | ID=MsG0780041652.01.T01:exon:20484;Parent=MsG0780041652.01.T01 |
| Chr7 | CDS | 93486328 | 93486729 | 93486328 | ID=MsG0780041652.01.T01:cds;Parent=MsG0780041652.01.T01 |
| Chr7 | CDS | 93486918 | 93487136 | 93486918 | ID=MsG0780041652.01.T01:cds;Parent=MsG0780041652.01.T01 |
| Gene Sequence |
| Protein sequence |
