AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007912.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007912.01.T01 MTR_2g035190 96.815 157 5 0 1 157 1 157 3.32e-107 302
MsG0280007912.01.T01 MTR_2g035320 95.541 157 7 0 1 157 1 157 8.34e-107 301
MsG0280007912.01.T01 MTR_2g035210 91.083 157 14 0 1 157 1 157 3.07e-101 287
MsG0280007912.01.T01 MTR_2g035220 89.809 157 16 0 1 157 1 157 1.41e-98 281
MsG0280007912.01.T01 MTR_6g033450 66.242 157 53 0 1 157 1 157 2.91e-70 209
MsG0280007912.01.T01 MTR_4g120970 66.242 157 53 0 1 157 1 157 2.91e-70 209
MsG0280007912.01.T01 MTR_2g035100 63.694 157 57 0 1 157 1 157 9.87e-68 202
MsG0280007912.01.T01 MTR_2g035105 63.057 157 58 0 1 157 1 157 8.34e-67 200
MsG0280007912.01.T01 MTR_2g035150 63.694 157 57 0 1 157 1 157 8.81e-67 200
MsG0280007912.01.T01 MTR_2g035130 61.783 157 60 0 1 157 1 157 1.69e-65 197
MsG0280007912.01.T01 MTR_2g035120 59.236 157 64 0 1 157 1 157 3.26e-62 189
MsG0280007912.01.T01 MTR_2g035320 90.000 100 10 0 58 157 23 122 3.12e-61 185
MsG0280007912.01.T01 MTR_2g035170 49.045 157 80 0 1 157 1 157 9.91e-50 157
MsG0280007912.01.T01 MTR_4g120950 42.857 161 85 5 1 157 1 158 3.74e-32 112
MsG0280007912.01.T01 MTR_2g435310 32.051 156 105 1 1 155 1 156 8.55e-30 106
MsG0280007912.01.T01 MTR_4g120760 33.758 157 103 1 1 156 1 157 1.30e-29 106
MsG0280007912.01.T01 MTR_4g120760 33.758 157 103 1 1 156 1 157 1.42e-29 106
MsG0280007912.01.T01 MTR_4g120940 65.753 73 25 0 85 157 47 119 1.07e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAATTGGCTAGTAATT+AGG 0.164037 2:-22823484 None:intergenic
GTTTAATTGGCTAGTAATTA+GGG 0.278519 2:-22823483 None:intergenic
GGATAGCTTCTTAATAGTTC+CGG 0.311830 2:-22822496 None:intergenic
TACAGCTTAGTGGGAGGAAC+AGG 0.324066 2:+22823268 MsG0280007912.01.T01:CDS
CTTGATAAGTCCAGTTCCTT+TGG 0.329858 2:-22823434 None:intergenic
TTCAACAATTTCAACACTTT+GGG 0.350751 2:-22822460 None:intergenic
CTTCAACAATTTCAACACTT+TGG 0.364915 2:-22822461 None:intergenic
TGGGCAGCAGAAATCACCTT+TGG 0.387864 2:-22822441 None:intergenic
AGATAGAGTTGCATCACCTT+TGG 0.394288 2:-22823386 None:intergenic
AGAGCTTTGTAGAGTTTAGC+TGG 0.394931 2:-22822393 None:intergenic
GATAGCTTCTTAATAGTTCC+GGG 0.397748 2:-22822495 None:intergenic
GTTGAAATTGTTGAAGGAAA+TGG 0.402462 2:+22822468 MsG0280007912.01.T01:CDS
GAGACTAAGGCCAAAGGAAC+TGG 0.415366 2:+22823424 MsG0280007912.01.T01:CDS
GGGCAGCAGAAATCACCTTT+GGG 0.449440 2:-22822440 None:intergenic
TCAGTGGATGAGGCAAACTT+TGG 0.464386 2:+22823238 MsG0280007912.01.T01:CDS
TGCTAAAACGTAGCCCTCAA+TGG 0.474394 2:-22823458 None:intergenic
TGGATATAACTACAGCTTAG+TGG 0.476164 2:+22823258 MsG0280007912.01.T01:CDS
AAAATTGTTGCTGGCTCTGA+TGG 0.476544 2:+22823328 MsG0280007912.01.T01:CDS
CAAAGTGTTGAAATTGTTGA+AGG 0.502542 2:+22822462 MsG0280007912.01.T01:CDS
CAAATTAGATTCAGTGGATG+AGG 0.505903 2:+22823228 MsG0280007912.01.T01:CDS
GGATATAACTACAGCTTAGT+GGG 0.506709 2:+22823259 MsG0280007912.01.T01:CDS
ATCTGAATTACCTTCAACAA+TGG 0.509250 2:-22822517 None:intergenic
TAATTTGTGTAGCACAAAGT+TGG 0.512097 2:-22823212 None:intergenic
GGACTTATCAAGGCCATTGA+GGG 0.514208 2:+22823445 MsG0280007912.01.T01:CDS
CAAAGGAACTGGACTTATCA+AGG 0.516806 2:+22823435 MsG0280007912.01.T01:CDS
AAGAAGCTATCCATTGTTGA+AGG 0.523757 2:+22822507 MsG0280007912.01.T01:CDS
AGCAGTACGTGAGGAGACTA+AGG 0.536209 2:+22823411 MsG0280007912.01.T01:CDS
GAGTTGCATCACCTTTGGTA+TGG 0.537356 2:-22823381 None:intergenic
GCTACACAAATTAGATTCAG+TGG 0.559555 2:+22823222 MsG0280007912.01.T01:CDS
TTTCACTGAAATCTTAACAA+TGG 0.567421 2:-22823356 None:intergenic
ATTGTTGCTGGCTCTGATGG+TGG 0.570543 2:+22823331 MsG0280007912.01.T01:CDS
TGGACTTATCAAGGCCATTG+AGG 0.572485 2:+22823444 MsG0280007912.01.T01:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2:+22822477 MsG0280007912.01.T01:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2:-22822385 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2:+22822425 MsG0280007912.01.T01:CDS
CTTAGTGGGAGGAACAGGGT+TGG 0.609437 2:+22823273 MsG0280007912.01.T01:CDS
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2:+22822471 MsG0280007912.01.T01:CDS
TCAGTGAAATACCATACCAA+AGG 0.652605 2:+22823370 MsG0280007912.01.T01:CDS
CGTGAGGAGACTAAGGCCAA+AGG 0.670663 2:+22823418 MsG0280007912.01.T01:CDS
TCTATCTGAAGCAGTACGTG+AGG 0.676642 2:+22823402 MsG0280007912.01.T01:CDS
ACAGCTTAGTGGGAGGAACA+GGG 0.680453 2:+22823269 MsG0280007912.01.T01:CDS
TATAACTACAGCTTAGTGGG+AGG 0.697586 2:+22823262 MsG0280007912.01.T01:CDS
TGATGAACATGTCTCAACCG+TGG 0.717160 2:+22822368 MsG0280007912.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATAAATAATTATAA+TGG + Chr2:22823169-22823188 MsG0280007912.01.T01:intron 0.0%
!!! AAATAAAAGTATAACATAAA+TGG - Chr2:22822874-22822893 None:intergenic 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - Chr2:22822662-22822681 None:intergenic 10.0%
!!! ATTTAACTTCCTTTTTTTTT+TGG - Chr2:22822799-22822818 None:intergenic 15.0%
!!! ATTTTATACCCAAAAAAAAA+AGG + Chr2:22822787-22822806 MsG0280007912.01.T01:intron 15.0%
!!! TAATTTAACTTTCTTTTCTT+TGG - Chr2:22822767-22822786 None:intergenic 15.0%
!!! TTTAACTTCCTTTTTTTTTT+GGG - Chr2:22822798-22822817 None:intergenic 15.0%
!! AATAGTAATCCAATTCTATT+TGG + Chr2:22822836-22822855 MsG0280007912.01.T01:intron 20.0%
! AATAGAATCTTGAGAAATGA+AGG + Chr2:22822897-22822916 MsG0280007912.01.T01:intron 25.0%
! AGGGTTATGTCTAATAATTA+TGG + Chr2:22822942-22822961 MsG0280007912.01.T01:intron 25.0%
! ATAATTATTAGACATAACCC+TGG - Chr2:22822943-22822962 None:intergenic 25.0%
! TATAATGGTTATATTGCAGA+TGG + Chr2:22823184-22823203 MsG0280007912.01.T01:intron 25.0%
! TTCAACAATTTCAACACTTT+GGG - Chr2:22822463-22822482 None:intergenic 25.0%
! TTCAGATGATTAATGATACT+CGG + Chr2:22823082-22823101 MsG0280007912.01.T01:intron 25.0%
! TTTCACTGAAATCTTAACAA+TGG - Chr2:22823359-22823378 None:intergenic 25.0%
!!! CATCTGAATCATTTTTTTGA+AGG - Chr2:22822969-22822988 None:intergenic 25.0%
ATCTGAATTACCTTCAACAA+TGG - Chr2:22822520-22822539 None:intergenic 30.0%
CATGACTATCCAAATAGAAT+TGG - Chr2:22822848-22822867 None:intergenic 30.0%
CTTCAACAATTTCAACACTT+TGG - Chr2:22822464-22822483 None:intergenic 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + Chr2:22822468-22822487 MsG0280007912.01.T01:CDS 30.0%
TTGTACCAACAAACTTTGAT+CGG - Chr2:22822621-22822640 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - Chr2:22823215-22823234 None:intergenic 30.0%
! TTTGAGACAAAAATTGTTGC+TGG + Chr2:22823319-22823338 MsG0280007912.01.T01:CDS 30.0%
!! CAAAGTGTTGAAATTGTTGA+AGG + Chr2:22822462-22822481 MsG0280007912.01.T01:CDS 30.0%
AACAAACTTTGATCGGTTGT+CGG - Chr2:22822614-22822633 None:intergenic 35.0%
AAGAAGCTATCCATTGTTGA+AGG + Chr2:22822507-22822526 MsG0280007912.01.T01:CDS 35.0%
ATAATACATAAGCTCGTTCC+AGG + Chr2:22822922-22822941 MsG0280007912.01.T01:intron 35.0%
CAAATTAGATTCAGTGGATG+AGG + Chr2:22823228-22823247 MsG0280007912.01.T01:CDS 35.0%
GAAATTGTTGAAGGAAATGG+AGG + Chr2:22822471-22822490 MsG0280007912.01.T01:CDS 35.0%
GACAAACATGATGTGACAAA+TGG + Chr2:22822676-22822695 MsG0280007912.01.T01:intron 35.0%
GATAGCTTCTTAATAGTTCC+GGG - Chr2:22822498-22822517 None:intergenic 35.0%
GCTACACAAATTAGATTCAG+TGG + Chr2:22823222-22823241 MsG0280007912.01.T01:CDS 35.0%
GGATAGCTTCTTAATAGTTC+CGG - Chr2:22822499-22822518 None:intergenic 35.0%
GGATATAACTACAGCTTAGT+GGG + Chr2:22823259-22823278 MsG0280007912.01.T01:CDS 35.0%
TAATACATAAGCTCGTTCCA+GGG + Chr2:22822923-22822942 MsG0280007912.01.T01:intron 35.0%
TCAGTGAAATACCATACCAA+AGG + Chr2:22823370-22823389 MsG0280007912.01.T01:CDS 35.0%
TGGATATAACTACAGCTTAG+TGG + Chr2:22823258-22823277 MsG0280007912.01.T01:CDS 35.0%
AGATAGAGTTGCATCACCTT+TGG - Chr2:22823389-22823408 None:intergenic 40.0%
CAAAGGAACTGGACTTATCA+AGG + Chr2:22823435-22823454 MsG0280007912.01.T01:CDS 40.0%
CTTGATAAGTCCAGTTCCTT+TGG - Chr2:22823437-22823456 None:intergenic 40.0%
GACAACCGATCAAAGTTTGT+TGG + Chr2:22822613-22822632 MsG0280007912.01.T01:intron 40.0%
TATAACTACAGCTTAGTGGG+AGG + Chr2:22823262-22823281 MsG0280007912.01.T01:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - Chr2:22822396-22822415 None:intergenic 40.0%
!! AAAATTGTTGCTGGCTCTGA+TGG + Chr2:22823328-22823347 MsG0280007912.01.T01:CDS 40.0%
GAGTTGCATCACCTTTGGTA+TGG - Chr2:22823384-22823403 None:intergenic 45.0%
GGACTTATCAAGGCCATTGA+GGG + Chr2:22823445-22823464 MsG0280007912.01.T01:CDS 45.0%
TCAGTGGATGAGGCAAACTT+TGG + Chr2:22823238-22823257 MsG0280007912.01.T01:CDS 45.0%
TGATGAACATGTCTCAACCG+TGG + Chr2:22822368-22822387 MsG0280007912.01.T01:CDS 45.0%
TGCTAAAACGTAGCCCTCAA+TGG - Chr2:22823461-22823480 None:intergenic 45.0%
TGCTGATGAAATCGTCCCAA+AGG + Chr2:22822425-22822444 MsG0280007912.01.T01:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + Chr2:22823444-22823463 MsG0280007912.01.T01:CDS 45.0%
! TCTATCTGAAGCAGTACGTG+AGG + Chr2:22823402-22823421 MsG0280007912.01.T01:CDS 45.0%
ACAGCTTAGTGGGAGGAACA+GGG + Chr2:22823269-22823288 MsG0280007912.01.T01:CDS 50.0%
AGCAGTACGTGAGGAGACTA+AGG + Chr2:22823411-22823430 MsG0280007912.01.T01:CDS 50.0%
GAGACTAAGGCCAAAGGAAC+TGG + Chr2:22823424-22823443 MsG0280007912.01.T01:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - Chr2:22822443-22822462 None:intergenic 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG - Chr2:22822388-22822407 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + Chr2:22822477-22822496 MsG0280007912.01.T01:CDS 50.0%
TACAGCTTAGTGGGAGGAAC+AGG + Chr2:22823268-22823287 MsG0280007912.01.T01:CDS 50.0%
TGGGCAGCAGAAATCACCTT+TGG - Chr2:22822444-22822463 None:intergenic 50.0%
!! ATTGTTGCTGGCTCTGATGG+TGG + Chr2:22823331-22823350 MsG0280007912.01.T01:CDS 50.0%
CGTGAGGAGACTAAGGCCAA+AGG + Chr2:22823418-22823437 MsG0280007912.01.T01:CDS 55.0%
CTTAGTGGGAGGAACAGGGT+TGG + Chr2:22823273-22823292 MsG0280007912.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22822348 22823495 22822348 ID=MsG0280007912.01;Name=MsG0280007912.01
Chr2 mRNA 22822348 22823495 22822348 ID=MsG0280007912.01.T01;Parent=MsG0280007912.01;Name=MsG0280007912.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|157
Chr2 exon 22822348 22822528 22822348 ID=MsG0280007912.01.T01:exon:13878;Parent=MsG0280007912.01.T01
Chr2 exon 22823203 22823495 22823203 ID=MsG0280007912.01.T01:exon:13879;Parent=MsG0280007912.01.T01
Chr2 CDS 22822348 22822528 22822348 ID=MsG0280007912.01.T01:cds;Parent=MsG0280007912.01.T01
Chr2 CDS 22823203 22823495 22823203 ID=MsG0280007912.01.T01:cds;Parent=MsG0280007912.01.T01
Gene Sequence

>MsG0280007912.01.T01

ATGGGTGTTTTTACTTTCAATGATGAACATGTCTCAACCGTGGCTCCAGCTAAACTCTACAAAGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAAGTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACTATTAAGAAGCTATCCATTGTTGAAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATTCAGTGGATGAGGCAAACTTTGGATATAACTACAGCTTAGTGGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTTGAGACAAAAATTGTTGCTGGCTCTGATGGTGGATCCATTGTTAAGATTTCAGTGAAATACCATACCAAAGGTGATGCAACTCTATCTGAAGCAGTACGTGAGGAGACTAAGGCCAAAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTTTTAGCAAACCCTAATTACTAG

Protein sequence

>MsG0280007912.01.T01

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQSVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDSVDEANFGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIVKISVKYHTKGDATLSEAVREETKAKGTGLIKAIEGYVLANPNY*