AlfalfaGEDB | MsG0880042150.01

M. sativa cultivar ZhongmuNo.1 / MsG0880042150.01

KEGG: K19825
GO: -
PFAM: PF16884

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042150.01.T01	XP_039684678.1	71.512	344	10	3	1	256	1	344	1.39E-162	464

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042150.01.T01	Q39172	53.216	342	68	6	6	256	5	345	4.65E-114	333

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042150.01.T01	B7FL02	71.512	344	10	3	1	256	1	344	6.64e-163	464

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048809.01	MsG0880042150.01	-0.809401	1.927109e-50	1.130380e-47
MsG0180004001.01	MsG0880042150.01	0.805014	1.637942e-49	8.571570e-47
MsG0180005354.01	MsG0880042150.01	0.801754	7.757604e-49	3.734279e-46
MsG0180005446.01	MsG0880042150.01	0.814095	1.834575e-51	1.219554e-48
MsG0280011287.01	MsG0880042150.01	0.802314	5.949358e-49	2.905301e-46
MsG0880042150.01	MsG0880042151.01	0.821015	5.054199e-53	4.064209e-50
MsG0880042150.01	MsG0880042223.01	0.910402	1.950072e-82	3.975031e-78

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042150.01.T01	MTR_8g010000	69.906	319	33	3	1	256	1	319	2.72e-151	424
MsG0880042150.01.T01	MTR_8g010030	64.244	344	35	3	1	256	1	344	2.61e-148	418
MsG0880042150.01.T01	MTR_8g009820	60.580	345	45	5	1	256	60	402	1.84e-134	385
MsG0880042150.01.T01	MTR_8g009980	59.130	345	51	5	1	256	1	344	3.45e-134	382
MsG0880042150.01.T01	MTR_8g010020	62.791	344	8	4	1	256	1	312	8.96e-134	380
MsG0880042150.01.T01	MTR_8g009830	57.971	345	53	6	1	256	1	342	8.51e-130	371
MsG0880042150.01.T01	MTR_8g009840	58.407	339	49	6	1	250	1	336	5.59e-125	365
MsG0880042150.01.T01	MTR_4g082970	50.292	342	80	5	4	256	10	350	6.08e-99	293
MsG0880042150.01.T01	MTR_8g007325	65.625	192	37	3	1	165	1	190	2.86e-83	249
MsG0880042150.01.T01	MTR_7g406860	43.103	348	102	7	1	256	3	346	9.55e-80	244

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880042150.01.T01	AT5G16970	53.216	342	68	6	6	256	5	345	4.74e-115	333
MsG0880042150.01.T01	AT5G17000	53.509	342	67	6	6	256	5	345	3.61e-114	332
MsG0880042150.01.T01	AT5G16990	53.372	341	68	6	6	256	4	343	2.23e-112	327
MsG0880042150.01.T01	AT1G26320	53.097	339	72	6	4	256	9	346	8.02e-110	320
MsG0880042150.01.T01	AT3G03080	51.734	346	74	6	2	256	7	350	4.12e-109	319
MsG0880042150.01.T01	AT1G26320	52.326	344	72	6	4	256	9	351	4.96e-109	318
MsG0880042150.01.T01	AT5G16960	48.986	345	84	6	1	254	1	344	4.36e-103	303
MsG0880042150.01.T01	AT5G16960	48.986	345	84	6	1	254	1	344	4.36e-103	303
MsG0880042150.01.T01	AT5G37940	48.105	343	85	6	6	256	12	353	5.74e-101	298
MsG0880042150.01.T01	AT5G38000	50.309	324	85	7	6	255	12	333	7.45e-101	297

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Find 67 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGGTGATATTGTGTG+GGG	0.630696	8:-4380252	MsG0880042150.01.T01:CDS
AAAGGCCCGGAAGCCTTGGT+TGG	0.564505	8:-4378342	MsG0880042150.01.T01:CDS
AAATTACATGGTCGCATACC+TGG	0.503064	8:-4378552	MsG0880042150.01.T01:intron
AACCATGCAATTTAGCGAAT+TGG	0.343765	8:+4379447	None:intergenic
AAGAAGGTGATATTGTGTGG+GGG	0.693745	8:-4380251	MsG0880042150.01.T01:CDS
AAGTAAACGTCAATGCCTTC+TGG	0.294778	8:+4378621	None:intergenic
AAGTTGACCAACTGCTCCAG+AGG	0.627539	8:+4379475	None:intergenic
AATAACAAGCCCTCTTTGTA+TGG	0.380288	8:-4378231	MsG0880042150.01.T01:three_prime_UTR
AATATGCCAACCAAGGCTTC+CGG	0.359999	8:+4378336	None:intergenic
ACAACATCGTTGGAACCTTC+TGG	0.330331	8:+4381282	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAAAACATTCTCCTTTCT+TGG	+	Chr8:4380032-4380051	None:intergenic	30.0%
ACGACACAAAAATGAATTTC+TGG	+	Chr8:4380596-4380615	None:intergenic	30.0%
ATAAAGAAGGTGATATTGTG+TGG	-	Chr8:4379281-4379300	MsG0880042150.01.T01:intron	30.0%
ATATTGAGGTCTGATTCTTT+AGG	+	Chr8:4378205-4378224	None:intergenic	30.0%
CAAAAACATTCTCCTTTCTT+GGG	+	Chr8:4380031-4380050	None:intergenic	30.0%
CACAATAATACACTTGATGT+CGG	-	Chr8:4379450-4379469	MsG0880042150.01.T01:CDS	30.0%
CATTGAACAAGAGAAGAAAA+TGG	+	Chr8:4379633-4379652	None:intergenic	30.0%
CTGTTTAGTATTGTAACAGA+CGG	-	Chr8:4379528-4379547	MsG0880042150.01.T01:intron	30.0%
GGAATAAGAATTGTTCTAGT+CGG	-	Chr8:4378614-4378633	MsG0880042150.01.T01:CDS	30.0%
GGACACAAAGATTATAAAGA+AGG	-	Chr8:4379268-4379287	MsG0880042150.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	4378148	4381409	4378148	ID=MsG0880042150.01;Name=MsG0880042150.01
Chr8	mRNA	4378148	4381409	4378148	ID=MsG0880042150.01.T01;Parent=MsG0880042150.01;Name=MsG0880042150.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0.25\|0.6\|1\|1\|1\|5\|139\|256
Chr8	exon	4378148	4378465	4378148	ID=MsG0880042150.01.T01:exon:2560;Parent=MsG0880042150.01.T01
Chr8	exon	4378553	4378650	4378553	ID=MsG0880042150.01.T01:exon:2559;Parent=MsG0880042150.01.T01
Chr8	exon	4379413	4379500	4379413	ID=MsG0880042150.01.T01:exon:2558;Parent=MsG0880042150.01.T01
Chr8	exon	4380151	4380328	4380151	ID=MsG0880042150.01.T01:exon:2557;Parent=MsG0880042150.01.T01
Chr8	exon	4381182	4381409	4381182	ID=MsG0880042150.01.T01:exon:2556;Parent=MsG0880042150.01.T01
Chr8	CDS	4381182	4381409	4381182	ID=MsG0880042150.01.T01:cds;Parent=MsG0880042150.01.T01
Chr8	CDS	4380151	4380328	4380151	ID=MsG0880042150.01.T01:cds;Parent=MsG0880042150.01.T01
Chr8	CDS	4379413	4379500	4379413	ID=MsG0880042150.01.T01:cds;Parent=MsG0880042150.01.T01
Chr8	CDS	4378553	4378650	4378553	ID=MsG0880042150.01.T01:cds;Parent=MsG0880042150.01.T01
Chr8	CDS	4378287	4378465	4378287	ID=MsG0880042150.01.T01:cds;Parent=MsG0880042150.01.T01
Chr8	three_prime_UTR	4378148	4378286	4378148	ID=MsG0880042150.01.T01:three_prime_utr;Parent=MsG0880042150.01.T01

Gene Sequence

>MsG0880042150.01.T01

ATGGCTCAAGTTAAGAACAAACAAGTGCTTCTGAAACATTACGTGTCTGGTTTTCCTAAAGAATCAGACCTCAATATTATTGACAGTACCGTAAGTCTTAAGCTTCCAGAAGGTTCCAACGATGTTGTTTTGGTTAAAAATCTTTACTTGTCCTGTGATCCTTACATGAGGACTCTTATGAGTGACCCAAAAGCTTCTATCAACCCACGTGCCTATGTTCCTCAATCTCCGATAACTGGTTATGGTGTGTCTAAAGTTCTGGAATCTGGACACAAAGATTATAAAGAAGGTGATATTGTGTGGGGGATAACTAATTGGGAAGAGTACAGCTTGATCCCTGCAGCTCAAATACATTTCAAAATTGAGCACACTGATGTTCCTCTTTCATACTATACTGGCATTCTTGCTGCCTCTGGAGCAGTTGGTCAACTTGTTGGCCAATTCGCTAAATTGCATGGTTGCTATGTCGTTGGAAGTGCTGGAAGTAAGGAGAGGCACTTTCCAGAAGGCATTGACGTTTACTTTGAGAACGTTGGAGGCAAGACACTTGATGCTGTACTCTTGAATATGAAATTACATGGTCGCATACCTGTTGATTACTATCATCTATATGCCAAATTCTTGGAGTTTGTCCTGCCTCATATCAGAGAAGGTAAGGTTGTGTATGTGGAAGACATAGCTGAGGGTCTTGAGAAAGGCCCGGAAGCCTTGGTTGGCATATTTAGTGGTAAAAATGTTGGCAAACAAGTGCTTGTTGTTGCTCATGAATGA

Protein sequence

>MsG0880042150.01.T01

MAQVKNKQVLLKHYVSGFPKESDLNIIDSTVSLKLPEGSNDVVLVKNLYLSCDPYMRTLMSDPKASINPRAYVPQSPITGYGVSKVLESGHKDYKEGDIVWGITNWEEYSLIPAAQIHFKIEHTDVPLSYYTGILAASGAVGQLVGQFAKLHGCYVVGSAGSKERHFPEGIDVYFENVGGKTLDAVLLNMKLHGRIPVDYYHLYAKFLEFVLPHIREGKVVYVEDIAEGLEKGPEALVGIFSGKNVGKQVLVVAHE*