AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005446.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005446.01.T01 MTR_1g100623 72.000 150 18 1 1 126 627 776 3.88e-62 202
MsG0180005446.01.T01 MTR_1g100623 72.000 150 18 1 1 126 675 824 4.48e-62 203
MsG0180005446.01.T01 MTR_1g100607 73.684 114 19 4 24 126 91 204 1.06e-44 145
MsG0180005446.01.T01 MTR_1g100627 61.240 129 18 3 22 126 396 516 8.19e-37 131
MsG0180005446.01.T01 MTR_6g045273 33.520 179 55 6 7 126 928 1101 2.19e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAGGGTTAAGAATTATTA+TGG 0.214950 1:+91968196 MsG0180005446.01.T01:CDS
GTGATTCTGTTACTTGTATT+AGG 0.290750 1:+91968501 MsG0180005446.01.T01:three_prime_UTR
ATATTATAGAGAAATTTATT+TGG 0.306548 1:-91968467 None:intergenic
TTAAGAATTATTATGGATCT+TGG 0.355305 1:+91968203 MsG0180005446.01.T01:CDS
GGCTACTGTGAAGGACTATC+TGG 0.384886 1:+91967996 MsG0180005446.01.T01:CDS
CAGGGAAGGGCATGGTTGAC+AGG 0.385822 1:+91968179 MsG0180005446.01.T01:CDS
TTGGTAACTTGATCTTCAAT+TGG 0.390183 1:-91968448 None:intergenic
GTAAAATGTGTAAGTTTGTA+TGG 0.405493 1:+91968543 MsG0180005446.01.T01:three_prime_UTR
TGTGGTGATTGATTATCCTC+AGG 0.467751 1:-91968235 None:intergenic
CTGTCAACCATGCCCTTCCC+TGG 0.467879 1:-91968178 None:intergenic
ACTTCTAAGCTCAGTAATGA+TGG 0.475886 1:+91967922 MsG0180005446.01.T01:CDS
ATACTATATCTAGTGTTGTA+AGG 0.476683 1:+91968599 MsG0180005446.01.T01:three_prime_UTR
TAAAGATCATGTTGCAGAAG+TGG 0.479475 1:+91968382 MsG0180005446.01.T01:CDS
AGTAATGATGGAACTGGCAC+TGG 0.498521 1:+91967934 MsG0180005446.01.T01:CDS
GAGAGCAATGTGAATAGTCC+AGG 0.510589 1:+91968160 MsG0180005446.01.T01:CDS
AAGGCCTAGTGAAGAAGATA+AGG 0.516903 1:+91968029 MsG0180005446.01.T01:CDS
AAGCTCAGTAATGATGGAAC+TGG 0.535037 1:+91967928 MsG0180005446.01.T01:CDS
ACTATCTGGCTGAGAAGCTA+AGG 0.538588 1:+91968010 MsG0180005446.01.T01:CDS
TGTGTAAGTTTGTATGGGTG+TGG 0.542234 1:+91968549 MsG0180005446.01.T01:three_prime_UTR
ACTGGCACTGGTAGTAATGA+AGG 0.560033 1:+91967946 MsG0180005446.01.T01:CDS
AGTGCCTTATCTTCTTCACT+AGG 0.566989 1:-91968033 None:intergenic
TATCTAGTGTTGTAAGGCTG+TGG 0.570542 1:+91968605 MsG0180005446.01.T01:three_prime_UTR
AGAAGATAAGGCACTTTCTG+AGG 0.578825 1:+91968041 MsG0180005446.01.T01:CDS
CAATGTGAATAGTCCAGGGA+AGG 0.585429 1:+91968165 MsG0180005446.01.T01:CDS
AGGGAAGGGCATGGTTGACA+GGG 0.590934 1:+91968180 MsG0180005446.01.T01:CDS
AGACAAAGGGGCTACTGTGA+AGG 0.610761 1:+91967987 MsG0180005446.01.T01:CDS
TAAAATGTGTAAGTTTGTAT+GGG 0.610935 1:+91968544 MsG0180005446.01.T01:three_prime_UTR
GTTGCAGAAGTGGAACAAGT+TGG 0.612425 1:+91968392 MsG0180005446.01.T01:CDS
ATACTTATAGGTGTAGACAT+TGG 0.616860 1:+91968341 MsG0180005446.01.T01:intron
ATCTTGGTTTGCTAAACCTG+AGG 0.626786 1:+91968219 MsG0180005446.01.T01:CDS
AGAGCAATGTGAATAGTCCA+GGG 0.631175 1:+91968161 MsG0180005446.01.T01:CDS
GAATAGTCCAGGGAAGGGCA+TGG 0.637293 1:+91968171 MsG0180005446.01.T01:CDS
GAAGTGGAACAAGTTGGCAA+AGG 0.645524 1:+91968398 MsG0180005446.01.T01:CDS
AATGTGAATAGTCCAGGGAA+GGG 0.651191 1:+91968166 MsG0180005446.01.T01:CDS
GGAACAAGTTGGCAAAGGTG+TGG 0.655366 1:+91968403 MsG0180005446.01.T01:CDS
GAGGATAATCAATCACCACA+AGG 0.728787 1:+91968238 MsG0180005446.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATAGAGAAATTTATT+TGG - Chr1:91968470-91968489 None:intergenic 10.0%
!! ATTTCAGAAACTTTACATAA+AGG + Chr1:91968066-91968085 MsG0180005446.01.T01:intron 20.0%
!! TAAAATGTGTAAGTTTGTAT+GGG + Chr1:91968544-91968563 MsG0180005446.01.T01:three_prime_UTR 20.0%
!! TTAAGAATTATTATGGATCT+TGG + Chr1:91968203-91968222 MsG0180005446.01.T01:CDS 20.0%
!! TTTGATGTTCTTATACTTAT+AGG + Chr1:91968329-91968348 MsG0180005446.01.T01:intron 20.0%
! AAACAAAAAATGGAGACAAA+GGG + Chr1:91967974-91967993 MsG0180005446.01.T01:CDS 25.0%
! ATACTATATCTAGTGTTGTA+AGG + Chr1:91968599-91968618 MsG0180005446.01.T01:three_prime_UTR 25.0%
! GTAAAATGTGTAAGTTTGTA+TGG + Chr1:91968543-91968562 MsG0180005446.01.T01:three_prime_UTR 25.0%
! TGAAAAAGAAGATGGAATAT+TGG + Chr1:91968102-91968121 MsG0180005446.01.T01:intron 25.0%
!! AGAAGTTTTCATATTCAAGA+GGG - Chr1:91967908-91967927 None:intergenic 25.0%
!! TAGAAGTTTTCATATTCAAG+AGG - Chr1:91967909-91967928 None:intergenic 25.0%
!!! TATTCCATCTTCTTTTTCAA+AGG - Chr1:91968101-91968120 None:intergenic 25.0%
!!! TTGTATTAGGTCTTTTTTCA+TGG + Chr1:91968514-91968533 MsG0180005446.01.T01:three_prime_UTR 25.0%
AACAAAAAATGGAGACAAAG+GGG + Chr1:91967975-91967994 MsG0180005446.01.T01:CDS 30.0%
AGAGAAAAAGAATTACCTTG+TGG - Chr1:91968256-91968275 None:intergenic 30.0%
ATACTTATAGGTGTAGACAT+TGG + Chr1:91968341-91968360 MsG0180005446.01.T01:intron 30.0%
GAAACAAAAAATGGAGACAA+AGG + Chr1:91967973-91967992 MsG0180005446.01.T01:CDS 30.0%
GAAGGAACTGAAACAAAAAA+TGG + Chr1:91967964-91967983 MsG0180005446.01.T01:CDS 30.0%
GACAGGGTTAAGAATTATTA+TGG + Chr1:91968196-91968215 MsG0180005446.01.T01:CDS 30.0%
! GTGATTCTGTTACTTGTATT+AGG + Chr1:91968501-91968520 MsG0180005446.01.T01:three_prime_UTR 30.0%
! TTGGTAACTTGATCTTCAAT+TGG - Chr1:91968451-91968470 None:intergenic 30.0%
ACTTCTAAGCTCAGTAATGA+TGG + Chr1:91967922-91967941 MsG0180005446.01.T01:CDS 35.0%
TAAAGATCATGTTGCAGAAG+TGG + Chr1:91968382-91968401 MsG0180005446.01.T01:CDS 35.0%
! AGAGCCTTTGAAAAAGAAGA+TGG + Chr1:91968094-91968113 MsG0180005446.01.T01:intron 35.0%
AAGGCCTAGTGAAGAAGATA+AGG + Chr1:91968029-91968048 MsG0180005446.01.T01:CDS 40.0%
AATGTGAATAGTCCAGGGAA+GGG + Chr1:91968166-91968185 MsG0180005446.01.T01:CDS 40.0%
AGAGCAATGTGAATAGTCCA+GGG + Chr1:91968161-91968180 MsG0180005446.01.T01:CDS 40.0%
AGTGCCTTATCTTCTTCACT+AGG - Chr1:91968036-91968055 None:intergenic 40.0%
GAGGATAATCAATCACCACA+AGG + Chr1:91968238-91968257 MsG0180005446.01.T01:CDS 40.0%
TATCTAGTGTTGTAAGGCTG+TGG + Chr1:91968605-91968624 MsG0180005446.01.T01:three_prime_UTR 40.0%
TGTGGTGATTGATTATCCTC+AGG - Chr1:91968238-91968257 None:intergenic 40.0%
TGTGTAAGTTTGTATGGGTG+TGG + Chr1:91968549-91968568 MsG0180005446.01.T01:three_prime_UTR 40.0%
! AAGCTCAGTAATGATGGAAC+TGG + Chr1:91967928-91967947 MsG0180005446.01.T01:CDS 40.0%
! AGAAGATAAGGCACTTTCTG+AGG + Chr1:91968041-91968060 MsG0180005446.01.T01:CDS 40.0%
! AGATGGAATATTGGCTAGTG+AGG + Chr1:91968111-91968130 MsG0180005446.01.T01:intron 40.0%
!! ATCTTGGTTTGCTAAACCTG+AGG + Chr1:91968219-91968238 MsG0180005446.01.T01:CDS 40.0%
ACTATCTGGCTGAGAAGCTA+AGG + Chr1:91968010-91968029 MsG0180005446.01.T01:CDS 45.0%
CAATGTGAATAGTCCAGGGA+AGG + Chr1:91968165-91968184 MsG0180005446.01.T01:CDS 45.0%
GAGAGCAATGTGAATAGTCC+AGG + Chr1:91968160-91968179 MsG0180005446.01.T01:CDS 45.0%
GTTGCAGAAGTGGAACAAGT+TGG + Chr1:91968392-91968411 MsG0180005446.01.T01:CDS 45.0%
! AGTAATGATGGAACTGGCAC+TGG + Chr1:91967934-91967953 MsG0180005446.01.T01:CDS 45.0%
! GAAGTGGAACAAGTTGGCAA+AGG + Chr1:91968398-91968417 MsG0180005446.01.T01:CDS 45.0%
!! ACTGGCACTGGTAGTAATGA+AGG + Chr1:91967946-91967965 MsG0180005446.01.T01:CDS 45.0%
AGACAAAGGGGCTACTGTGA+AGG + Chr1:91967987-91968006 MsG0180005446.01.T01:CDS 50.0%
GGCTACTGTGAAGGACTATC+TGG + Chr1:91967996-91968015 MsG0180005446.01.T01:CDS 50.0%
! GGAACAAGTTGGCAAAGGTG+TGG + Chr1:91968403-91968422 MsG0180005446.01.T01:CDS 50.0%
GAATAGTCCAGGGAAGGGCA+TGG + Chr1:91968171-91968190 MsG0180005446.01.T01:CDS 55.0%
! AGGGAAGGGCATGGTTGACA+GGG + Chr1:91968180-91968199 MsG0180005446.01.T01:CDS 55.0%
CTGTCAACCATGCCCTTCCC+TGG - Chr1:91968181-91968200 None:intergenic 60.0%
! CAGGGAAGGGCATGGTTGAC+AGG + Chr1:91968179-91968198 MsG0180005446.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 91967892 91968713 91967892 ID=MsG0180005446.01;Name=MsG0180005446.01
Chr1 mRNA 91967892 91968713 91967892 ID=MsG0180005446.01.T01;Parent=MsG0180005446.01;Name=MsG0180005446.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0.5|0.33|1|1|1|3|271|129
Chr1 exon 91967892 91968062 91967892 ID=MsG0180005446.01.T01:exon:15889;Parent=MsG0180005446.01.T01
Chr1 exon 91968133 91968259 91968133 ID=MsG0180005446.01.T01:exon:15890;Parent=MsG0180005446.01.T01
Chr1 exon 91968351 91968713 91968351 ID=MsG0180005446.01.T01:exon:15891;Parent=MsG0180005446.01.T01
Chr1 CDS 91967892 91968062 91967892 ID=MsG0180005446.01.T01:cds;Parent=MsG0180005446.01.T01
Chr1 CDS 91968133 91968259 91968133 ID=MsG0180005446.01.T01:cds;Parent=MsG0180005446.01.T01
Chr1 CDS 91968351 91968442 91968351 ID=MsG0180005446.01.T01:cds;Parent=MsG0180005446.01.T01
Chr1 three_prime_UTR 91968443 91968713 91968443 ID=MsG0180005446.01.T01:three_prime_utr;Parent=MsG0180005446.01.T01
Gene Sequence

>MsG0180005446.01.T01

ATGATAAAACTCTCCCTCTTGAATATGAAAACTTCTAAGCTCAGTAATGATGGAACTGGCACTGGTAGTAATGAAGGAACTGAAACAAAAAATGGAGACAAAGGGGCTACTGTGAAGGACTATCTGGCTGAGAAGCTAAGGCCTAGTGAAGAAGATAAGGCACTTTCTGAGGATGAGAAGTCAGAGAAAGTGTTTGAAGAGAGCAATGTGAATAGTCCAGGGAAGGGCATGGTTGACAGGGTTAAGAATTATTATGGATCTTGGTTTGCTAAACCTGAGGATAATCAATCACCACAAGGTGTAGACATTGGTGAAGAGTTATCAAAGAATAAAGATCATGTTGCAGAAGTGGAACAAGTTGGCAAAGGTGTGGATGAAGAAGACACATGA

Protein sequence

>MsG0180005446.01.T01

MIKLSLLNMKTSKLSNDGTGTGSNEGTETKNGDKGATVKDYLAEKLRPSEEDKALSEDEKSEKVFEESNVNSPGKGMVDRVKNYYGSWFAKPEDNQSPQGVDIGEELSKNKDHVAEVEQVGKGVDEEDT*