Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043031.01.T01 | KEH18682.1 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 3.74E-40 | 137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043031.01.T01 | A0A072TMN4 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 1.79e-40 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049008.01 | MsG0880043031.01 | 0.809951 | 1.468152e-50 | 8.737755e-48 |
| MsG0180002021.01 | MsG0880043031.01 | -0.812697 | 3.721662e-51 | 2.382522e-48 |
| MsG0180004064.01 | MsG0880043031.01 | 0.805819 | 1.110154e-49 | 5.931321e-47 |
| MsG0180004787.01 | MsG0880043031.01 | -0.804899 | 1.731215e-49 | 9.033898e-47 |
| MsG0880043031.01 | MsG0880044548.01 | -0.801351 | 9.379028e-49 | 4.469387e-46 |
| MsG0880043031.01 | MsG0880046010.01 | -0.822612 | 2.158645e-53 | 1.815980e-50 |
| MsG0880043031.01 | MsG0880046080.01 | 0.807949 | 3.937236e-50 | 2.222352e-47 |
| MsG0880043031.01 | MsG0880046341.01 | -0.806551 | 7.787664e-50 | 4.239210e-47 |
| MsG0280011281.01 | MsG0880043031.01 | -0.825148 | 5.488646e-54 | 4.962129e-51 |
| MsG0580028159.01 | MsG0880043031.01 | -0.814711 | 1.340351e-51 | 9.060186e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043031.01.T01 | MTR_8g027820 | 98.551 | 69 | 1 | 0 | 1 | 69 | 1 | 69 | 4.53e-44 | 137 |
| MsG0880043031.01.T01 | MTR_7g028975 | 82.609 | 69 | 12 | 0 | 1 | 69 | 1 | 69 | 1.71e-37 | 119 |
| MsG0880043031.01.T01 | MTR_8g097020 | 78.261 | 69 | 15 | 0 | 1 | 69 | 1 | 69 | 3.82e-36 | 116 |
| MsG0880043031.01.T01 | MTR_8g096840 | 63.768 | 69 | 25 | 0 | 1 | 69 | 1 | 69 | 1.16e-30 | 102 |
| MsG0880043031.01.T01 | MTR_3g085030 | 60.870 | 69 | 27 | 0 | 1 | 69 | 1 | 69 | 1.54e-29 | 99.8 |
| MsG0880043031.01.T01 | MTR_3g085020 | 63.768 | 69 | 25 | 0 | 1 | 69 | 1 | 69 | 3.20e-29 | 99.0 |
| MsG0880043031.01.T01 | MTR_7g028870 | 57.576 | 66 | 28 | 0 | 2 | 67 | 3 | 68 | 4.33e-23 | 83.6 |
| MsG0880043031.01.T01 | MTR_7g028940 | 50.725 | 69 | 34 | 0 | 1 | 69 | 1 | 69 | 7.22e-23 | 82.8 |
| MsG0880043031.01.T01 | MTR_8g097030 | 66.176 | 68 | 23 | 0 | 2 | 69 | 3 | 70 | 2.95e-21 | 79.0 |
| MsG0880043031.01.T01 | MTR_7g028815 | 57.692 | 52 | 22 | 0 | 1 | 52 | 1 | 52 | 2.28e-19 | 75.5 |
| MsG0880043031.01.T01 | MTR_7g028945 | 38.235 | 68 | 42 | 0 | 1 | 68 | 1 | 68 | 3.61e-13 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043031.01.T01 | AT5G08391 | 68.116 | 69 | 22 | 0 | 1 | 69 | 1 | 69 | 6.54e-34 | 110 |
| MsG0880043031.01.T01 | AT3G27030 | 68.182 | 66 | 21 | 0 | 1 | 66 | 1 | 66 | 2.55e-31 | 106 |
| MsG0880043031.01.T01 | AT3G27030 | 46.377 | 69 | 37 | 0 | 1 | 69 | 74 | 142 | 1.56e-20 | 79.0 |
| MsG0880043031.01.T01 | AT3G48660 | 65.217 | 69 | 24 | 0 | 1 | 69 | 1 | 69 | 5.11e-31 | 103 |
| MsG0880043031.01.T01 | AT5G63500 | 59.420 | 69 | 28 | 0 | 1 | 69 | 1 | 69 | 1.88e-28 | 97.1 |
| MsG0880043031.01.T01 | AT3G01950 | 61.538 | 65 | 25 | 0 | 3 | 67 | 4 | 68 | 2.58e-25 | 89.0 |
| MsG0880043031.01.T01 | AT5G14110 | 55.224 | 67 | 30 | 0 | 3 | 69 | 4 | 70 | 5.83e-24 | 85.5 |
| MsG0880043031.01.T01 | AT3G27027 | 54.545 | 66 | 30 | 0 | 2 | 67 | 3 | 68 | 1.02e-23 | 85.1 |
| MsG0880043031.01.T01 | AT5G40980 | 59.259 | 54 | 22 | 0 | 2 | 55 | 3 | 56 | 2.22e-20 | 76.6 |
| MsG0880043031.01.T01 | AT3G01940 | 53.731 | 67 | 31 | 0 | 2 | 68 | 4 | 70 | 4.95e-18 | 70.5 |
| MsG0880043031.01.T01 | AT5G50560 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 1.08e-14 | 62.0 |
| MsG0880043031.01.T01 | AT5G50660 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 1.08e-14 | 62.0 |
| MsG0880043031.01.T01 | AT5G40960 | 38.806 | 67 | 41 | 0 | 1 | 67 | 1 | 67 | 4.79e-11 | 52.8 |
Find 18 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGCCTTTACCAGGTATT+TGG | 0.173299 | 8:-17904468 | None:intergenic |
| CTCTTCATTCTCTTGACTCC+TGG | 0.292357 | 8:+17904436 | MsG0880043031.01.T01:CDS |
| CAAGATGGAATGAATGAGTA+TGG | 0.351813 | 8:-17904527 | None:intergenic |
| GTGAGTGAAGATGTCAGATT+GGG | 0.427895 | 8:+17904390 | None:intergenic |
| AAAGGCAAGATGGTTGAGTT+TGG | 0.428056 | 8:+17904481 | MsG0880043031.01.T01:CDS |
| AGTGAGTGAAGATGTCAGAT+TGG | 0.469480 | 8:+17904389 | None:intergenic |
| TTTGGCAACTTTCAAACTAG+TGG | 0.496595 | 8:+17904499 | MsG0880043031.01.T01:CDS |
| TCAACCATCTTGCCTTTACC+AGG | 0.504670 | 8:-17904475 | None:intergenic |
| CTTGTCCAAATACCTGGTAA+AGG | 0.523495 | 8:+17904463 | MsG0880043031.01.T01:CDS |
| TATTAGAATTCACATGTACA+TGG | 0.538362 | 8:+17904582 | MsG0880043031.01.T01:CDS |
| GGTTTGCTTGTCCAAATACC+TGG | 0.540826 | 8:+17904457 | MsG0880043031.01.T01:CDS |
| AACTAGAGCAAAATACAAGA+TGG | 0.561187 | 8:-17904542 | None:intergenic |
| GGTATTTGGACAAGCAAACC+AGG | 0.589146 | 8:-17904454 | None:intergenic |
| ATTAGAATTCACATGTACAT+GGG | 0.590111 | 8:+17904583 | MsG0880043031.01.T01:CDS |
| AATACCTGGTAAAGGCAAGA+TGG | 0.597351 | 8:+17904471 | MsG0880043031.01.T01:CDS |
| TTAGAATTCACATGTACATG+GGG | 0.674938 | 8:+17904584 | MsG0880043031.01.T01:CDS |
| TGAGTGAAGATGTCAGATTG+GGG | 0.690065 | 8:+17904391 | None:intergenic |
| GAGTGAAGATGTCAGATTGG+GGG | 0.700727 | 8:+17904392 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAGAATTCACATGTACAT+GGG | + | Chr8:17904583-17904602 | MsG0880043031.01.T01:CDS | 25.0% |
| ! | TATTAGAATTCACATGTACA+TGG | + | Chr8:17904582-17904601 | MsG0880043031.01.T01:CDS | 25.0% |
| AACTAGAGCAAAATACAAGA+TGG | - | Chr8:17904545-17904564 | None:intergenic | 30.0% | |
| TTAGAATTCACATGTACATG+GGG | + | Chr8:17904584-17904603 | MsG0880043031.01.T01:CDS | 30.0% | |
| CAAGATGGAATGAATGAGTA+TGG | - | Chr8:17904530-17904549 | None:intergenic | 35.0% | |
| TTTGGCAACTTTCAAACTAG+TGG | + | Chr8:17904499-17904518 | MsG0880043031.01.T01:CDS | 35.0% | |
| AAAGGCAAGATGGTTGAGTT+TGG | + | Chr8:17904481-17904500 | MsG0880043031.01.T01:CDS | 40.0% | |
| AATACCTGGTAAAGGCAAGA+TGG | + | Chr8:17904471-17904490 | MsG0880043031.01.T01:CDS | 40.0% | |
| CTTGTCCAAATACCTGGTAA+AGG | + | Chr8:17904463-17904482 | MsG0880043031.01.T01:CDS | 40.0% | |
| TCTTGCCTTTACCAGGTATT+TGG | - | Chr8:17904471-17904490 | None:intergenic | 40.0% | |
| AGCACCACTGACACAAAAAC+TGG | - | Chr8:17904418-17904437 | None:intergenic | 45.0% | |
| CTCTTCATTCTCTTGACTCC+TGG | + | Chr8:17904436-17904455 | MsG0880043031.01.T01:CDS | 45.0% | |
| GGTATTTGGACAAGCAAACC+AGG | - | Chr8:17904457-17904476 | None:intergenic | 45.0% | |
| TCAACCATCTTGCCTTTACC+AGG | - | Chr8:17904478-17904497 | None:intergenic | 45.0% | |
| ! | GGTTTGCTTGTCCAAATACC+TGG | + | Chr8:17904457-17904476 | MsG0880043031.01.T01:CDS | 45.0% |
| ! | GGGGCCAGTTTTTGTGTCAG+TGG | + | Chr8:17904411-17904430 | MsG0880043031.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 17904400 | 17904609 | 17904400 | ID=MsG0880043031.01;Name=MsG0880043031.01 |
| Chr8 | mRNA | 17904400 | 17904609 | 17904400 | ID=MsG0880043031.01.T01;Parent=MsG0880043031.01;Name=MsG0880043031.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|69 |
| Chr8 | exon | 17904400 | 17904609 | 17904400 | ID=MsG0880043031.01.T01:exon:5165;Parent=MsG0880043031.01.T01 |
| Chr8 | CDS | 17904400 | 17904609 | 17904400 | ID=MsG0880043031.01.T01:cds;Parent=MsG0880043031.01.T01 |
| Gene Sequence |
| Protein sequence |
