Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046080.01.T01 | KEH31582.1 | 97.143 | 175 | 4 | 1 | 29 | 203 | 113 | 286 | 9.06E-118 | 346 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046080.01.T01 | O81851 | 34.659 | 176 | 109 | 3 | 30 | 203 | 132 | 303 | 3.38E-22 | 94.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046080.01.T01 | A0A072UPG0 | 97.143 | 175 | 4 | 1 | 29 | 203 | 113 | 286 | 4.33e-118 | 346 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048887.01 | MsG0880046080.01 | -0.814012 | 1.912958e-51 | 1.268861e-48 |
| MsG0080049008.01 | MsG0880046080.01 | 0.847070 | 1.458664e-59 | 2.562436e-56 |
| MsG0080049024.01 | MsG0880046080.01 | -0.807319 | 5.355771e-50 | 2.973996e-47 |
| MsG0180000466.01 | MsG0880046080.01 | 0.829830 | 4.133429e-55 | 4.279808e-52 |
| MsG0180001004.01 | MsG0880046080.01 | 0.811407 | 7.109410e-51 | 4.397343e-48 |
| MsG0180001137.01 | MsG0880046080.01 | -0.805996 | 1.019376e-49 | 5.471102e-47 |
| MsG0180002238.01 | MsG0880046080.01 | 0.802578 | 5.249329e-49 | 2.580508e-46 |
| MsG0880044342.01 | MsG0880046080.01 | 0.832590 | 8.665021e-56 | 9.729104e-53 |
| MsG0880045703.01 | MsG0880046080.01 | -0.807513 | 4.871769e-50 | 2.718880e-47 |
| MsG0880045949.01 | MsG0880046080.01 | 0.805590 | 1.240263e-49 | 6.587311e-47 |
| MsG0880046010.01 | MsG0880046080.01 | -0.851884 | 6.634256e-61 | 1.361859e-57 |
| MsG0180003351.01 | MsG0880046080.01 | -0.803957 | 2.720613e-49 | 1.385571e-46 |
| MsG0180003405.01 | MsG0880046080.01 | -0.873575 | 1.322257e-67 | 5.766806e-64 |
| MsG0180003758.01 | MsG0880046080.01 | -0.800190 | 1.619106e-48 | 7.491524e-46 |
| MsG0180004058.01 | MsG0880046080.01 | 0.815248 | 1.019038e-51 | 6.988616e-49 |
| MsG0180004064.01 | MsG0880046080.01 | 0.830797 | 2.398672e-55 | 2.554065e-52 |
| MsG0180004714.01 | MsG0880046080.01 | -0.817726 | 2.839205e-52 | 2.084153e-49 |
| MsG0180004787.01 | MsG0880046080.01 | -0.808175 | 3.524523e-50 | 2.001443e-47 |
| MsG0180005666.01 | MsG0880046080.01 | -0.865232 | 6.856743e-65 | 2.214496e-61 |
| MsG0280006446.01 | MsG0880046080.01 | 0.815250 | 1.017894e-51 | 6.981341e-49 |
| MsG0780040640.01 | MsG0880046080.01 | -0.802384 | 5.756618e-49 | 2.816137e-46 |
| MsG0780040716.01 | MsG0880046080.01 | 0.800519 | 1.387185e-48 | 6.472411e-46 |
| MsG0780040847.01 | MsG0880046080.01 | 0.803695 | 3.083743e-49 | 1.559914e-46 |
| MsG0780040892.01 | MsG0880046080.01 | -0.824967 | 6.057556e-54 | 5.448107e-51 |
| MsG0780041403.01 | MsG0880046080.01 | 0.806216 | 9.160661e-50 | 4.944333e-47 |
| MsG0780041436.01 | MsG0880046080.01 | 0.804722 | 1.884261e-49 | 9.787582e-47 |
| MsG0880043031.01 | MsG0880046080.01 | 0.807949 | 3.937236e-50 | 2.222352e-47 |
| MsG0880043056.01 | MsG0880046080.01 | -0.838242 | 3.230362e-57 | 4.300475e-54 |
| MsG0280010584.01 | MsG0880046080.01 | -0.814275 | 1.673500e-51 | 1.117966e-48 |
| MsG0280011106.01 | MsG0880046080.01 | 0.805225 | 1.479359e-49 | 7.783663e-47 |
| MsG0280011268.01 | MsG0880046080.01 | 0.806790 | 6.932393e-50 | 3.796803e-47 |
| MsG0280011269.01 | MsG0880046080.01 | 0.813946 | 1.978698e-51 | 1.310037e-48 |
| MsG0280011281.01 | MsG0880046080.01 | -0.855455 | 6.238634e-62 | 1.441201e-58 |
| MsG0280011393.01 | MsG0880046080.01 | 0.805751 | 1.147348e-49 | 6.119612e-47 |
| MsG0380013843.01 | MsG0880046080.01 | 0.844434 | 7.576872e-59 | 1.223043e-55 |
| MsG0380014231.01 | MsG0880046080.01 | -0.813228 | 2.847027e-51 | 1.848474e-48 |
| MsG0380014639.01 | MsG0880046080.01 | -0.815426 | 9.301056e-52 | 6.410579e-49 |
| MsG0380014642.01 | MsG0880046080.01 | -0.804660 | 1.941981e-49 | 1.007037e-46 |
| MsG0680031449.01 | MsG0880046080.01 | -0.831582 | 1.538686e-55 | 1.677011e-52 |
| MsG0680032416.01 | MsG0880046080.01 | -0.809492 | 1.842494e-50 | 1.083308e-47 |
| MsG0480019171.01 | MsG0880046080.01 | 0.855793 | 4.968643e-62 | 1.160795e-58 |
| MsG0480019663.01 | MsG0880046080.01 | 0.804845 | 1.776031e-49 | 9.254629e-47 |
| MsG0480020269.01 | MsG0880046080.01 | -0.803399 | 3.551844e-49 | 1.783115e-46 |
| MsG0480020928.01 | MsG0880046080.01 | 0.804701 | 1.903772e-49 | 9.882684e-47 |
| MsG0480021967.01 | MsG0880046080.01 | -0.858576 | 7.495387e-63 | 1.921675e-59 |
| MsG0480022154.01 | MsG0880046080.01 | -0.816865 | 4.436024e-52 | 3.180339e-49 |
| MsG0480023103.01 | MsG0880046080.01 | -0.811908 | 5.533584e-51 | 3.468039e-48 |
| MsG0480023466.01 | MsG0880046080.01 | -0.823265 | 1.520497e-53 | 1.302774e-50 |
| MsG0480023851.01 | MsG0880046080.01 | -0.861442 | 1.023035e-63 | 2.895486e-60 |
| MsG0480023925.01 | MsG0880046080.01 | 0.824720 | 6.926659e-54 | 6.186198e-51 |
| MsG0580024980.01 | MsG0880046080.01 | 0.839437 | 1.585888e-57 | 2.190057e-54 |
| MsG0580025633.01 | MsG0880046080.01 | 0.828632 | 8.071201e-55 | 8.067730e-52 |
| MsG0380016456.01 | MsG0880046080.01 | -0.803392 | 3.564142e-49 | 1.788913e-46 |
| MsG0380016490.01 | MsG0880046080.01 | 0.806632 | 7.485881e-50 | 4.083078e-47 |
| MsG0380017594.01 | MsG0880046080.01 | 0.805400 | 1.359559e-49 | 7.185748e-47 |
| MsG0380017640.01 | MsG0880046080.01 | 0.800801 | 1.215632e-48 | 5.712470e-46 |
| MsG0480018202.01 | MsG0880046080.01 | -0.810595 | 1.065908e-50 | 6.452135e-48 |
| MsG0480018203.01 | MsG0880046080.01 | -0.804866 | 1.758554e-49 | 9.168591e-47 |
| MsG0480018338.01 | MsG0880046080.01 | -0.828933 | 6.824136e-55 | 6.880882e-52 |
| MsG0580027276.01 | MsG0880046080.01 | -0.833757 | 4.440081e-56 | 5.162387e-53 |
| MsG0580028159.01 | MsG0880046080.01 | -0.803905 | 2.788680e-49 | 1.418377e-46 |
| MsG0580028302.01 | MsG0880046080.01 | 0.806792 | 6.926375e-50 | 3.793688e-47 |
| MsG0580029158.01 | MsG0880046080.01 | 0.828844 | 7.173596e-55 | 7.214063e-52 |
| MsG0580029751.01 | MsG0880046080.01 | -0.838579 | 2.644790e-57 | 3.557813e-54 |
| MsG0680030296.01 | MsG0880046080.01 | 0.813692 | 2.251035e-51 | 1.480172e-48 |
| MsG0280006560.01 | MsG0880046080.01 | 0.806089 | 9.741962e-50 | 5.240918e-47 |
| MsG0280007248.01 | MsG0880046080.01 | -0.813927 | 1.998100e-51 | 1.322199e-48 |
| MsG0280007505.01 | MsG0880046080.01 | 0.819405 | 1.181562e-52 | 9.084001e-50 |
| MsG0280007653.01 | MsG0880046080.01 | -0.802228 | 6.196714e-49 | 3.019237e-46 |
| MsG0280009526.01 | MsG0880046080.01 | 0.815308 | 9.879924e-52 | 6.787389e-49 |
| MsG0780036726.01 | MsG0880046080.01 | 0.824162 | 9.371123e-54 | 8.235611e-51 |
| MsG0780037999.01 | MsG0880046080.01 | 0.826744 | 2.293035e-54 | 2.169216e-51 |
| MsG0780038647.01 | MsG0880046080.01 | 0.814032 | 1.893497e-51 | 1.256633e-48 |
| MsG0780038914.01 | MsG0880046080.01 | 0.809849 | 1.543620e-50 | 9.162349e-48 |
| MsG0780038919.01 | MsG0880046080.01 | 0.816330 | 5.847595e-52 | 4.131463e-49 |
| MsG0780039234.01 | MsG0880046080.01 | 0.827107 | 1.878052e-54 | 1.795541e-51 |
| MsG0780039287.01 | MsG0880046080.01 | -0.829056 | 6.371310e-55 | 6.447529e-52 |
| MsG0780039619.01 | MsG0880046080.01 | 0.843342 | 1.485951e-58 | 2.318162e-55 |
| MsG0780040154.01 | MsG0880046080.01 | -0.804267 | 2.345069e-49 | 1.203782e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046080.01.T01 | MTR_4g099060 | 97.143 | 175 | 4 | 1 | 29 | 203 | 113 | 286 | 1.10e-121 | 346 |
| MsG0880046080.01.T01 | MTR_4g099060 | 97.143 | 175 | 4 | 1 | 29 | 203 | 158 | 331 | 1.94e-121 | 347 |
| MsG0880046080.01.T01 | MTR_5g013690 | 63.687 | 179 | 59 | 3 | 29 | 203 | 96 | 272 | 6.82e-74 | 224 |
| MsG0880046080.01.T01 | MTR_5g013690 | 63.687 | 179 | 59 | 3 | 29 | 203 | 159 | 335 | 1.51e-73 | 225 |
| MsG0880046080.01.T01 | MTR_4g083540 | 34.091 | 176 | 108 | 3 | 30 | 203 | 124 | 293 | 5.39e-27 | 104 |
| MsG0880046080.01.T01 | MTR_1g019250 | 32.292 | 192 | 113 | 5 | 18 | 202 | 159 | 340 | 2.95e-26 | 103 |
| MsG0880046080.01.T01 | MTR_2g028830 | 30.811 | 185 | 116 | 3 | 26 | 203 | 140 | 319 | 1.34e-21 | 90.5 |
| MsG0880046080.01.T01 | MTR_4g036485 | 29.379 | 177 | 105 | 3 | 29 | 201 | 50 | 210 | 4.43e-21 | 87.0 |
| MsG0880046080.01.T01 | MTR_3g117970 | 33.333 | 174 | 93 | 5 | 29 | 202 | 45 | 195 | 5.46e-21 | 86.7 |
| MsG0880046080.01.T01 | MTR_2g069350 | 28.910 | 211 | 111 | 5 | 30 | 203 | 158 | 366 | 6.04e-21 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046080.01.T01 | AT5G47050 | 51.795 | 195 | 73 | 4 | 18 | 203 | 118 | 300 | 1.33e-55 | 179 |
| MsG0880046080.01.T01 | AT1G32740 | 48.438 | 192 | 83 | 4 | 18 | 202 | 85 | 267 | 4.43e-53 | 171 |
| MsG0880046080.01.T01 | AT1G32740 | 48.438 | 192 | 83 | 4 | 18 | 202 | 85 | 267 | 4.43e-53 | 171 |
| MsG0880046080.01.T01 | AT1G32740 | 48.438 | 192 | 83 | 4 | 18 | 202 | 129 | 311 | 9.64e-53 | 171 |
| MsG0880046080.01.T01 | AT1G32740 | 48.438 | 192 | 83 | 4 | 18 | 202 | 118 | 300 | 1.27e-52 | 171 |
| MsG0880046080.01.T01 | AT4G17680 | 48.045 | 179 | 83 | 4 | 29 | 202 | 17 | 190 | 2.14e-46 | 151 |
| MsG0880046080.01.T01 | AT4G17680 | 46.316 | 190 | 88 | 5 | 18 | 202 | 62 | 242 | 3.00e-46 | 153 |
| MsG0880046080.01.T01 | AT4G17680 | 48.045 | 179 | 83 | 4 | 29 | 202 | 141 | 314 | 6.95e-46 | 154 |
| MsG0880046080.01.T01 | AT1G10650 | 32.105 | 190 | 121 | 3 | 18 | 203 | 98 | 283 | 7.66e-31 | 114 |
| MsG0880046080.01.T01 | AT1G10650 | 32.955 | 176 | 114 | 2 | 32 | 203 | 164 | 339 | 1.19e-30 | 115 |
| MsG0880046080.01.T01 | AT1G60610 | 29.121 | 182 | 123 | 2 | 27 | 202 | 158 | 339 | 6.78e-26 | 102 |
| MsG0880046080.01.T01 | AT1G60610 | 29.121 | 182 | 123 | 2 | 27 | 202 | 158 | 339 | 6.78e-26 | 102 |
| MsG0880046080.01.T01 | AT1G60610 | 29.121 | 182 | 123 | 2 | 27 | 202 | 158 | 339 | 6.78e-26 | 102 |
| MsG0880046080.01.T01 | AT1G60610 | 29.121 | 182 | 123 | 2 | 27 | 202 | 158 | 339 | 6.78e-26 | 102 |
| MsG0880046080.01.T01 | AT1G60610 | 29.121 | 182 | 123 | 2 | 27 | 202 | 158 | 339 | 6.78e-26 | 102 |
| MsG0880046080.01.T01 | AT1G45976 | 30.270 | 185 | 119 | 2 | 18 | 202 | 151 | 325 | 1.58e-24 | 98.6 |
| MsG0880046080.01.T01 | AT4G19700 | 34.659 | 176 | 109 | 3 | 30 | 203 | 132 | 303 | 3.45e-23 | 94.7 |
| MsG0880046080.01.T01 | AT1G79110 | 29.744 | 195 | 117 | 3 | 27 | 203 | 165 | 357 | 8.73e-23 | 94.4 |
| MsG0880046080.01.T01 | AT4G35070 | 32.461 | 191 | 111 | 6 | 18 | 202 | 31 | 209 | 1.48e-22 | 90.9 |
| MsG0880046080.01.T01 | AT1G79110 | 29.592 | 196 | 118 | 3 | 26 | 203 | 139 | 332 | 2.32e-22 | 92.8 |
| MsG0880046080.01.T01 | AT4G35070 | 33.520 | 179 | 105 | 5 | 30 | 202 | 94 | 264 | 2.34e-22 | 91.7 |
| MsG0880046080.01.T01 | AT5G45100 | 33.523 | 176 | 111 | 2 | 30 | 203 | 95 | 266 | 2.55e-22 | 91.7 |
| MsG0880046080.01.T01 | AT3G12920 | 28.947 | 190 | 114 | 4 | 30 | 203 | 150 | 334 | 2.94e-22 | 92.8 |
| MsG0880046080.01.T01 | AT1G79110 | 29.592 | 196 | 118 | 3 | 26 | 203 | 161 | 354 | 2.98e-22 | 92.8 |
| MsG0880046080.01.T01 | AT5G45100 | 33.523 | 176 | 111 | 2 | 30 | 203 | 122 | 293 | 4.05e-22 | 91.7 |
Find 68 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAAGCTGCTGCGGCTTCT+TGG | 0.157550 | 8:+68779024 | None:intergenic |
| AGAAGCTGCTGCGGCTTCTT+GGG | 0.245232 | 8:+68779025 | None:intergenic |
| ACAATAACAATAAATTATTT+AGG | 0.246703 | 8:+68778656 | None:intergenic |
| GACCTCAACGGTTGAATTCT+TGG | 0.268543 | 8:+68778734 | None:intergenic |
| CTTGGGCTTCCATGGTTAGC+TGG | 0.282676 | 8:+68779069 | None:intergenic |
| TTCTTCTAAACAACAATAAC+AGG | 0.299401 | 8:-68780029 | MsG0880046080.01.T01:CDS |
| ACATTATTGTCTGTTGCAGC+TGG | 0.306389 | 8:+68778994 | None:intergenic |
| CTGCTGCGGCTTCTTGGGCC+CGG | 0.325466 | 8:+68779030 | None:intergenic |
| GCAACTTCAACCACTCTATC+TGG | 0.325865 | 8:+68778885 | None:intergenic |
| CGCCAGCATCTTCGCCGGTT+TGG | 0.337158 | 8:+68778970 | None:intergenic |
| TTTAGAAGAAGAATATTTGA+TGG | 0.355647 | 8:+68780042 | None:intergenic |
| TGCTGCGGCTTCTTGGGCCC+GGG | 0.374579 | 8:+68779031 | None:intergenic |
| CCACTTCTTTCTCTCTTAAC+CGG | 0.391453 | 8:+68779141 | None:intergenic |
| CTGCCATACTTGGGCTTCCA+TGG | 0.410747 | 8:+68779061 | None:intergenic |
| TAACCATGGAAGCCCAAGTA+TGG | 0.415275 | 8:-68779064 | MsG0880046080.01.T01:CDS |
| TTCTTGGGAGTAAAGCAAAC+AGG | 0.420487 | 8:+68778750 | None:intergenic |
| ACCTCAACGGTTGAATTCTT+GGG | 0.433219 | 8:+68778735 | None:intergenic |
| GTTGAAGTTGCGGCGGCGCG+TGG | 0.434737 | 8:-68778873 | MsG0880046080.01.T01:CDS |
| CGGAGGAAGCGGTGGCGCAC+CGG | 0.460350 | 8:-68779160 | MsG0880046080.01.T01:CDS |
| TGCCAAACCGGCGAAGATGC+TGG | 0.461739 | 8:-68778972 | MsG0880046080.01.T01:CDS |
| CAGACAATAATGTGCCAAAC+CGG | 0.470211 | 8:-68778984 | MsG0880046080.01.T01:CDS |
| CATTATTGTCTGTTGCAGCT+GGG | 0.471488 | 8:+68778995 | None:intergenic |
| AAGTATGGCAGGCAAAAGCC+CGG | 0.473882 | 8:-68779049 | MsG0880046080.01.T01:CDS |
| TGGATCAATATAAGCCGATT+CGG | 0.478396 | 8:+68778905 | None:intergenic |
| TTGGGCTTCCATGGTTAGCT+GGG | 0.482097 | 8:+68779070 | None:intergenic |
| ATTGTTCATATACTAGTCTC+TGG | 0.486629 | 8:-68778638 | MsG0880046080.01.T01:three_prime_UTR |
| GGCGAAGATGCTGGCGCCGG+CGG | 0.491297 | 8:-68778963 | MsG0880046080.01.T01:CDS |
| CGCGGATTTAGAAGCGCGTA+CGG | 0.500807 | 8:-68779093 | MsG0880046080.01.T01:CDS |
| ACTAAACGCGGCGGAGGAAG+CGG | 0.504819 | 8:-68779171 | MsG0880046080.01.T01:CDS |
| GCCGGCGCCAGCATCTTCGC+CGG | 0.511102 | 8:+68778965 | None:intergenic |
| TTCATGCGGCATGGAAGGAC+AGG | 0.513128 | 8:-68778937 | MsG0880046080.01.T01:CDS |
| ACAGGCTGAGGATGCCGAAT+CGG | 0.514420 | 8:-68778919 | MsG0880046080.01.T01:CDS |
| GGTGTTTCATGCGGCATGGA+AGG | 0.515929 | 8:-68778942 | MsG0880046080.01.T01:CDS |
| GCGGCGCGTGGGAAATGTAG+AGG | 0.525683 | 8:-68778861 | MsG0880046080.01.T01:CDS |
| AGAAAATCTACGTCGGACAT+TGG | 0.525889 | 8:-68779225 | MsG0880046080.01.T01:CDS |
| ACCGGCGAAGATGCTGGCGC+CGG | 0.529216 | 8:-68778966 | MsG0880046080.01.T01:CDS |
| GTGTGAAAAGCGCGTGGCAA+CGG | 0.533579 | 8:-68778838 | MsG0880046080.01.T01:CDS |
| CATGGAAGCCCAAGTATGGC+AGG | 0.538619 | 8:-68779060 | MsG0880046080.01.T01:CDS |
| AGATAGAGTGGTTGAAGTTG+CGG | 0.555336 | 8:-68778883 | MsG0880046080.01.T01:CDS |
| TTGAAGTTGCGGCGGCGCGT+GGG | 0.555769 | 8:-68778872 | MsG0880046080.01.T01:CDS |
| GGCGCCGGCGGTGTTTCATG+CGG | 0.557650 | 8:-68778951 | MsG0880046080.01.T01:CDS |
| GTCGGACATTGGCGGAGAAG+AGG | 0.560211 | 8:-68779214 | MsG0880046080.01.T01:CDS |
| TAAATCCGCGTTCCTGCGAG+TGG | 0.563827 | 8:+68779106 | None:intergenic |
| CGGCGGTGTTTCATGCGGCA+TGG | 0.569832 | 8:-68778946 | MsG0880046080.01.T01:CDS |
| AAATCTACGTCGGACATTGG+CGG | 0.575341 | 8:-68779222 | MsG0880046080.01.T01:CDS |
| CCGCCGCGTTTAGTAGCTCT+CGG | 0.577766 | 8:+68779180 | None:intergenic |
| CGGCATGGAAGGACAGGCTG+AGG | 0.579309 | 8:-68778931 | MsG0880046080.01.T01:CDS |
| AATTCGCAAAAGCCACTCGC+AGG | 0.588745 | 8:-68779118 | MsG0880046080.01.T01:CDS |
| CGGCGCGTGGGAAATGTAGA+GGG | 0.594790 | 8:-68778860 | MsG0880046080.01.T01:CDS |
| TCCCAAGAATTCAACCGTTG+AGG | 0.595204 | 8:-68778736 | MsG0880046080.01.T01:CDS |
| CAAACACACAAATGCCGACA+AGG | 0.601757 | 8:+68778804 | None:intergenic |
| AGAGCTACTAAACGCGGCGG+AGG | 0.604882 | 8:-68779177 | MsG0880046080.01.T01:CDS |
| AGTATGGCAGGCAAAAGCCC+GGG | 0.608050 | 8:-68779048 | MsG0880046080.01.T01:CDS |
| TGAAAAGCGCGTGGCAACGG+TGG | 0.613660 | 8:-68778835 | MsG0880046080.01.T01:CDS |
| AAAAGCCACTCGCAGGAACG+CGG | 0.614760 | 8:-68779111 | MsG0880046080.01.T01:CDS |
| AAACGCGGCGGAGGAAGCGG+TGG | 0.615069 | 8:-68779168 | MsG0880046080.01.T01:CDS |
| CATGCCGCATGAAACACCGC+CGG | 0.618246 | 8:+68778947 | None:intergenic |
| TTATATTGATCCAGATAGAG+TGG | 0.622605 | 8:-68778895 | MsG0880046080.01.T01:CDS |
| GGCTTGCAGAGAAGCTGCTG+CGG | 0.629802 | 8:+68779016 | None:intergenic |
| CCGGTTAAGAGAGAAAGAAG+TGG | 0.657511 | 8:-68779141 | MsG0880046080.01.T01:CDS |
| TCTTGGGAGTAAAGCAAACA+GGG | 0.657653 | 8:+68778751 | None:intergenic |
| GCGTACGGCCCAGCTAACCA+TGG | 0.665693 | 8:-68779078 | MsG0880046080.01.T01:CDS |
| TAGAGTGGTTGAAGTTGCGG+CGG | 0.668773 | 8:-68778880 | MsG0880046080.01.T01:CDS |
| TAGAGGGTGTGAAAAGCGCG+TGG | 0.693793 | 8:-68778844 | MsG0880046080.01.T01:CDS |
| TTCAGGGAGAAAATCTACGT+CGG | 0.694834 | 8:-68779232 | MsG0880046080.01.T01:intron |
| CTAAGAGAGAAAGACCTCAA+CGG | 0.702666 | 8:+68778722 | None:intergenic |
| TTACCGAGAGCTACTAAACG+CGG | 0.757971 | 8:-68779183 | MsG0880046080.01.T01:CDS |
| CCGAGAGCTACTAAACGCGG+CGG | 0.776946 | 8:-68779180 | MsG0880046080.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAACAATAAATTATTT+AGG | + | Chr8:68780017-68780036 | None:intergenic | 10.0% |
| !!! | TTAGTATTTATTTCTTTTTT+TGG | + | Chr8:68779980-68779999 | None:intergenic | 10.0% |
| !!! | AAATCGAAAATTTTGAAAAT+TGG | + | Chr8:68779363-68779382 | None:intergenic | 15.0% |
| !!! | AATCGAAAATTTTGAAAATT+GGG | + | Chr8:68779362-68779381 | None:intergenic | 15.0% |
| !! | AGAAATGATTAATAACGTTA+GGG | + | Chr8:68779024-68779043 | None:intergenic | 20.0% |
| !! | TCATATAGAATTGTATTTGA+GGG | + | Chr8:68778705-68778724 | None:intergenic | 20.0% |
| !! | TTTAGAAGAAGAATATTTGA+TGG | + | Chr8:68778631-68778650 | None:intergenic | 20.0% |
| !! | TTTGAATGTTTATATGTGAT+GGG | - | Chr8:68779412-68779431 | MsG0880046080.01.T01:intron | 20.0% |
| !!! | ATTTTCGATTTATTCGAAAA+TGG | - | Chr8:68779372-68779391 | MsG0880046080.01.T01:intron | 20.0% |
| !!! | TTTTGAATGTTTATATGTGA+TGG | - | Chr8:68779411-68779430 | MsG0880046080.01.T01:intron | 20.0% |
| ! | GAAAGATGAATAATTTGAGA+AGG | + | Chr8:68779061-68779080 | None:intergenic | 25.0% |
| ! | GAAATGATTAATAACGTTAG+GGG | + | Chr8:68779023-68779042 | None:intergenic | 25.0% |
| ! | GAACAAGAAAAGAGATTAAT+TGG | + | Chr8:68778741-68778760 | None:intergenic | 25.0% |
| ! | GAGAAATGATTAATAACGTT+AGG | + | Chr8:68779025-68779044 | None:intergenic | 25.0% |
| ! | GTCATATAGAATTGTATTTG+AGG | + | Chr8:68778706-68778725 | None:intergenic | 25.0% |
| ! | TTCTTCTAAACAACAATAAC+AGG | - | Chr8:68778641-68778660 | MsG0880046080.01.T01:three_prime_UTR | 25.0% |
| !! | AATTGGGTTGTTAAAAAAGA+AGG | + | Chr8:68779346-68779365 | None:intergenic | 25.0% |
| !!! | GTTGTTGATGATGATTATTA+TGG | + | Chr8:68779090-68779109 | None:intergenic | 25.0% |
| !!! | TTGTTTTGTTTTGTTTACCT+GGG | + | Chr8:68779310-68779329 | None:intergenic | 25.0% |
| !!! | TTTGTTTTGTTTTGTTTACC+TGG | + | Chr8:68779311-68779330 | None:intergenic | 25.0% |
| ATTTGAGAAACAATTCCTTG+TGG | + | Chr8:68779249-68779268 | None:intergenic | 30.0% | |
| GTGACAAAGAAGAATGAAAA+GGG | + | Chr8:68778933-68778952 | None:intergenic | 30.0% | |
| GTTGATCATCGAATGATAAA+CGG | + | Chr8:68779134-68779153 | None:intergenic | 30.0% | |
| TGAATAATTTGAGAAGGTTG+AGG | + | Chr8:68779055-68779074 | None:intergenic | 30.0% | |
| TGTGACAAAGAAGAATGAAA+AGG | + | Chr8:68778934-68778953 | None:intergenic | 30.0% | |
| TTATATTGATCCAGATAGAG+TGG | - | Chr8:68779775-68779794 | MsG0880046080.01.T01:intron | 30.0% | |
| TTCTCATCTTTATTACCACA+AGG | - | Chr8:68779231-68779250 | MsG0880046080.01.T01:intron | 30.0% | |
| ! | ATTGTTCATATACTAGTCTC+TGG | - | Chr8:68780032-68780051 | MsG0880046080.01.T01:CDS | 30.0% |
| !! | TATATGTGATGGGTATTTCA+GGG | - | Chr8:68779422-68779441 | MsG0880046080.01.T01:intron | 30.0% |
| !! | TTATATGTGATGGGTATTTC+AGG | - | Chr8:68779421-68779440 | MsG0880046080.01.T01:intron | 30.0% |
| ACAACAAATGTTGTCTCTAC+TGG | - | Chr8:68779105-68779124 | MsG0880046080.01.T01:CDS | 35.0% | |
| CAATCTCAACAACAACAACA+AGG | - | Chr8:68779192-68779211 | MsG0880046080.01.T01:CDS | 35.0% | |
| TGGATCAATATAAGCCGATT+CGG | + | Chr8:68779768-68779787 | None:intergenic | 35.0% | |
| ! | ACATACTTGTGTTGTTGCAT+AGG | + | Chr8:68778884-68778903 | None:intergenic | 35.0% |
| ! | GTTGTTGTTGTTGAGATTGT+TGG | + | Chr8:68779191-68779210 | None:intergenic | 35.0% |
| ! | TCTTTTCCTAACAGCAGAAA+TGG | - | Chr8:68778759-68778778 | MsG0880046080.01.T01:CDS | 35.0% |
| AACAGCAGAAATGGACAAGA+AGG | - | Chr8:68778768-68778787 | MsG0880046080.01.T01:CDS | 40.0% | |
| ACAGCAGAAATGGACAAGAA+GGG | - | Chr8:68778769-68778788 | MsG0880046080.01.T01:CDS | 40.0% | |
| ACATTATTGTCTGTTGCAGC+TGG | + | Chr8:68779679-68779698 | None:intergenic | 40.0% | |
| ACCTCAACGGTTGAATTCTT+GGG | + | Chr8:68779938-68779957 | None:intergenic | 40.0% | |
| AGAAAATCTACGTCGGACAT+TGG | - | Chr8:68779445-68779464 | MsG0880046080.01.T01:intron | 40.0% | |
| AGATAGAGTGGTTGAAGTTG+CGG | - | Chr8:68779787-68779806 | MsG0880046080.01.T01:intron | 40.0% | |
| CAACAACAACAACAACCATC+AGG | - | Chr8:68778810-68778829 | MsG0880046080.01.T01:CDS | 40.0% | |
| CAGACAATAATGTGCCAAAC+CGG | - | Chr8:68779686-68779705 | MsG0880046080.01.T01:intron | 40.0% | |
| CATTATTGTCTGTTGCAGCT+GGG | + | Chr8:68779678-68779697 | None:intergenic | 40.0% | |
| CCACTTCTTTCTCTCTTAAC+CGG | + | Chr8:68779532-68779551 | None:intergenic | 40.0% | |
| CTAAGAGAGAAAGACCTCAA+CGG | + | Chr8:68779951-68779970 | None:intergenic | 40.0% | |
| GATCATCGAATGATAAACGG+AGG | + | Chr8:68779131-68779150 | None:intergenic | 40.0% | |
| TCTTGGGAGTAAAGCAAACA+GGG | + | Chr8:68779922-68779941 | None:intergenic | 40.0% | |
| TCTTGTCCATTTCTGCTGTT+AGG | + | Chr8:68778768-68778787 | None:intergenic | 40.0% | |
| TTACCTGGGTTTGAAGATAG+TGG | + | Chr8:68779296-68779315 | None:intergenic | 40.0% | |
| TTCAGGGAGAAAATCTACGT+CGG | - | Chr8:68779438-68779457 | MsG0880046080.01.T01:intron | 40.0% | |
| TTCTTGGGAGTAAAGCAAAC+AGG | + | Chr8:68779923-68779942 | None:intergenic | 40.0% | |
| AAATCTACGTCGGACATTGG+CGG | - | Chr8:68779448-68779467 | MsG0880046080.01.T01:intron | 45.0% | |
| AGACCACTATCTTCAAACCC+AGG | - | Chr8:68779290-68779309 | MsG0880046080.01.T01:intron | 45.0% | |
| ATCATGTCTCAACTCACCAC+AGG | - | Chr8:68778962-68778981 | MsG0880046080.01.T01:CDS | 45.0% | |
| CAAACACACAAATGCCGACA+AGG | + | Chr8:68779869-68779888 | None:intergenic | 45.0% | |
| CCGGTTAAGAGAGAAAGAAG+TGG | - | Chr8:68779529-68779548 | MsG0880046080.01.T01:intron | 45.0% | |
| CTTGCGAGAATTTGTACCTG+TGG | + | Chr8:68778981-68779000 | None:intergenic | 45.0% | |
| GACCTCAACGGTTGAATTCT+TGG | + | Chr8:68779939-68779958 | None:intergenic | 45.0% | |
| GATAGTCCTAATGCTCCTGA+TGG | + | Chr8:68778828-68778847 | None:intergenic | 45.0% | |
| GCAACTTCAACCACTCTATC+TGG | + | Chr8:68779788-68779807 | None:intergenic | 45.0% | |
| GGTACAAATTCTCGCAAGAG+AGG | - | Chr8:68778983-68779002 | MsG0880046080.01.T01:CDS | 45.0% | |
| TAACCATGGAAGCCCAAGTA+TGG | - | Chr8:68779606-68779625 | MsG0880046080.01.T01:intron | 45.0% | |
| TCCCAAGAATTCAACCGTTG+AGG | - | Chr8:68779934-68779953 | MsG0880046080.01.T01:intron | 45.0% | |
| TTACCGAGAGCTACTAAACG+CGG | - | Chr8:68779487-68779506 | MsG0880046080.01.T01:intron | 45.0% | |
| !! | CAACAACCATCAGGAGCATT+AGG | - | Chr8:68778819-68778838 | MsG0880046080.01.T01:CDS | 45.0% |
| !! | CGGTGGTTGTTTTACCTTGT+CGG | - | Chr8:68779852-68779871 | MsG0880046080.01.T01:intron | 45.0% |
| AAGTATGGCAGGCAAAAGCC+CGG | - | Chr8:68779621-68779640 | MsG0880046080.01.T01:intron | 50.0% | |
| AATTCGCAAAAGCCACTCGC+AGG | - | Chr8:68779552-68779571 | MsG0880046080.01.T01:intron | 50.0% | |
| TAGAGTGGTTGAAGTTGCGG+CGG | - | Chr8:68779790-68779809 | MsG0880046080.01.T01:intron | 50.0% | |
| TCTCGCAAGAGAGGAAGAGA+AGG | - | Chr8:68778992-68779011 | MsG0880046080.01.T01:CDS | 50.0% | |
| ! | GGCTTTTGCCTGCCATACTT+GGG | + | Chr8:68779621-68779640 | None:intergenic | 50.0% |
| ! | TTGGGCTTCCATGGTTAGCT+GGG | + | Chr8:68779603-68779622 | None:intergenic | 50.0% |
| AAAAGCCACTCGCAGGAACG+CGG | - | Chr8:68779559-68779578 | MsG0880046080.01.T01:intron | 55.0% | |
| ACAGGCTGAGGATGCCGAAT+CGG | - | Chr8:68779751-68779770 | MsG0880046080.01.T01:intron | 55.0% | |
| AGAAGCTGCTGCGGCTTCTT+GGG | + | Chr8:68779648-68779667 | None:intergenic | 55.0% | |
| AGTATGGCAGGCAAAAGCCC+GGG | - | Chr8:68779622-68779641 | MsG0880046080.01.T01:intron | 55.0% | |
| CATGGAAGCCCAAGTATGGC+AGG | - | Chr8:68779610-68779629 | MsG0880046080.01.T01:intron | 55.0% | |
| CTGCCATACTTGGGCTTCCA+TGG | + | Chr8:68779612-68779631 | None:intergenic | 55.0% | |
| CTTGGGCTTCCATGGTTAGC+TGG | + | Chr8:68779604-68779623 | None:intergenic | 55.0% | |
| GGTGTTTCATGCGGCATGGA+AGG | - | Chr8:68779728-68779747 | MsG0880046080.01.T01:intron | 55.0% | |
| TAAATCCGCGTTCCTGCGAG+TGG | + | Chr8:68779567-68779586 | None:intergenic | 55.0% | |
| TTCATGCGGCATGGAAGGAC+AGG | - | Chr8:68779733-68779752 | MsG0880046080.01.T01:intron | 55.0% | |
| ! | CGCGGATTTAGAAGCGCGTA+CGG | - | Chr8:68779577-68779596 | MsG0880046080.01.T01:intron | 55.0% |
| ! | GGGCTTTTGCCTGCCATACT+TGG | + | Chr8:68779622-68779641 | None:intergenic | 55.0% |
| ! | GTGTGAAAAGCGCGTGGCAA+CGG | - | Chr8:68779832-68779851 | MsG0880046080.01.T01:intron | 55.0% |
| ! | TAGAGGGTGTGAAAAGCGCG+TGG | - | Chr8:68779826-68779845 | MsG0880046080.01.T01:intron | 55.0% |
| ACTAAACGCGGCGGAGGAAG+CGG | - | Chr8:68779499-68779518 | MsG0880046080.01.T01:intron | 60.0% | |
| AGAGCTACTAAACGCGGCGG+AGG | - | Chr8:68779493-68779512 | MsG0880046080.01.T01:intron | 60.0% | |
| CATGCCGCATGAAACACCGC+CGG | + | Chr8:68779726-68779745 | None:intergenic | 60.0% | |
| CCGAGAGCTACTAAACGCGG+CGG | - | Chr8:68779490-68779509 | MsG0880046080.01.T01:intron | 60.0% | |
| CCGCCGCGTTTAGTAGCTCT+CGG | + | Chr8:68779493-68779512 | None:intergenic | 60.0% | |
| CGGCGCGTGGGAAATGTAGA+GGG | - | Chr8:68779810-68779829 | MsG0880046080.01.T01:intron | 60.0% | |
| GAGAAGCTGCTGCGGCTTCT+TGG | + | Chr8:68779649-68779668 | None:intergenic | 60.0% | |
| GGCTTGCAGAGAAGCTGCTG+CGG | + | Chr8:68779657-68779676 | None:intergenic | 60.0% | |
| GTCGGACATTGGCGGAGAAG+AGG | - | Chr8:68779456-68779475 | MsG0880046080.01.T01:intron | 60.0% | |
| TGCCAAACCGGCGAAGATGC+TGG | - | Chr8:68779698-68779717 | MsG0880046080.01.T01:intron | 60.0% | |
| ! | TGAAAAGCGCGTGGCAACGG+TGG | - | Chr8:68779835-68779854 | MsG0880046080.01.T01:intron | 60.0% |
| CGGCATGGAAGGACAGGCTG+AGG | - | Chr8:68779739-68779758 | MsG0880046080.01.T01:intron | 65.0% | |
| CGGCGGTGTTTCATGCGGCA+TGG | - | Chr8:68779724-68779743 | MsG0880046080.01.T01:intron | 65.0% | |
| GCGGCGCGTGGGAAATGTAG+AGG | - | Chr8:68779809-68779828 | MsG0880046080.01.T01:intron | 65.0% | |
| GCGTACGGCCCAGCTAACCA+TGG | - | Chr8:68779592-68779611 | MsG0880046080.01.T01:intron | 65.0% | |
| TTGAAGTTGCGGCGGCGCGT+GGG | - | Chr8:68779798-68779817 | MsG0880046080.01.T01:intron | 65.0% | |
| ! | CGCCAGCATCTTCGCCGGTT+TGG | + | Chr8:68779703-68779722 | None:intergenic | 65.0% |
| AAACGCGGCGGAGGAAGCGG+TGG | - | Chr8:68779502-68779521 | MsG0880046080.01.T01:intron | 70.0% | |
| ACCGGCGAAGATGCTGGCGC+CGG | - | Chr8:68779704-68779723 | MsG0880046080.01.T01:intron | 70.0% | |
| CTGCTGCGGCTTCTTGGGCC+CGG | + | Chr8:68779643-68779662 | None:intergenic | 70.0% | |
| GTTGAAGTTGCGGCGGCGCG+TGG | - | Chr8:68779797-68779816 | MsG0880046080.01.T01:intron | 70.0% | |
| TGCTGCGGCTTCTTGGGCCC+GGG | + | Chr8:68779642-68779661 | None:intergenic | 70.0% | |
| !! | GGCGCCGGCGGTGTTTCATG+CGG | - | Chr8:68779719-68779738 | MsG0880046080.01.T01:intron | 70.0% |
| CGGAGGAAGCGGTGGCGCAC+CGG | - | Chr8:68779510-68779529 | MsG0880046080.01.T01:intron | 75.0% | |
| GCCGGCGCCAGCATCTTCGC+CGG | + | Chr8:68779708-68779727 | None:intergenic | 75.0% | |
| ! | GGCGAAGATGCTGGCGCCGG+CGG | - | Chr8:68779707-68779726 | MsG0880046080.01.T01:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 68778607 | 68780085 | 68778607 | ID=MsG0880046080.01;Name=MsG0880046080.01 |
| Chr8 | mRNA | 68778607 | 68780085 | 68778607 | ID=MsG0880046080.01.T01;Parent=MsG0880046080.01;Name=MsG0880046080.01.T01;_AED=0.31;_eAED=0.33;_QI=0|0|0.5|1|0|0.5|2|115|203 |
| Chr8 | exon | 68778607 | 68779249 | 68778607 | ID=MsG0880046080.01.T01:exon:32630;Parent=MsG0880046080.01.T01 |
| Chr8 | exon | 68780002 | 68780085 | 68780002 | ID=MsG0880046080.01.T01:exon:32629;Parent=MsG0880046080.01.T01 |
| Chr8 | CDS | 68780002 | 68780085 | 68780002 | ID=MsG0880046080.01.T01:cds;Parent=MsG0880046080.01.T01 |
| Chr8 | CDS | 68778722 | 68779249 | 68778722 | ID=MsG0880046080.01.T01:cds;Parent=MsG0880046080.01.T01 |
| Chr8 | three_prime_UTR | 68778607 | 68778721 | 68778607 | ID=MsG0880046080.01.T01:three_prime_utr;Parent=MsG0880046080.01.T01 |
| Gene Sequence |
| Protein sequence |
