AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043455.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043455.01.T01 MTR_8g036035 74.541 381 26 3 1 329 1 362 0.0 546
MsG0880043455.01.T01 MTR_8g036020 71.467 375 12 3 3 329 1 328 0.0 506
MsG0880043455.01.T01 MTR_8g036040 61.765 340 83 2 37 329 19 358 6.67e-150 426
MsG0880043455.01.T01 MTR_1g088775 57.988 338 94 4 39 329 30 366 2.94e-135 389
MsG0880043455.01.T01 MTR_7g075090 57.440 336 95 4 41 329 32 366 8.32e-133 382
MsG0880043455.01.T01 MTR_8g036050 59.547 309 73 3 68 329 4 307 1.86e-123 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043455.01.T01 AT2G45440 59.365 315 81 3 62 329 51 365 6.28e-132 380
MsG0880043455.01.T01 AT3G60880 59.164 311 80 3 66 329 54 364 1.71e-131 379
MsG0880043455.01.T01 AT3G60880 59.164 311 80 3 66 329 55 365 2.20e-131 379

Find 94 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGACAAAGTTAAAGTTAT+TGG 0.153195 8:-25062909 MsG0880043455.01.T01:CDS
GAGGGTAGGGTTTGTGAATT+TGG 0.165923 8:-25062318 MsG0880043455.01.T01:CDS
TTTGTTAAGTTTAGAATAAC+TGG 0.217819 8:-25063582 MsG0880043455.01.T01:CDS
GGTCATCCCATGTCATTAAT+TGG 0.227162 8:+25062967 None:intergenic
TCAATCTGTTGCAAGCAATT+TGG 0.279572 8:-25062525 MsG0880043455.01.T01:CDS
AGCTTATGACTTTGTTTGAT+TGG 0.288548 8:-25062445 MsG0880043455.01.T01:CDS
GCTCACACAGTTAATTGTTT+TGG 0.290265 8:-25062930 MsG0880043455.01.T01:CDS
GTTGCAAGCAATTTGGTTCC+TGG 0.298102 8:-25062518 MsG0880043455.01.T01:CDS
TCATTCGTTCAGGTCGTATT+TGG 0.298249 8:-25064019 MsG0880043455.01.T01:five_prime_UTR
GTTGAAGGTATTCTTGTTGC+TGG 0.326426 8:-25063005 MsG0880043455.01.T01:CDS
CGCAATCCCATCTAGCTTCA+TGG 0.335386 8:+25062561 None:intergenic
AACCTTACAGCATGTGTTCC+AGG 0.337957 8:+25063435 None:intergenic
AACACTGCTCTTGCTCAACT+TGG 0.339067 8:-25062392 MsG0880043455.01.T01:CDS
CGGCCGTTGAGCTGCTCAAC+TGG 0.346160 8:+25063380 None:intergenic
TTATTCTTATTCATTCGTTC+AGG 0.357920 8:-25064029 None:intergenic
ATTCTTGTTGCTGGCTCAAC+TGG 0.359387 8:-25062996 MsG0880043455.01.T01:CDS
TTAATGAGGGAACTCATGTT+TGG 0.363241 8:-25062494 MsG0880043455.01.T01:CDS
GAAAGGTAAAACCGGTGAAT+TGG 0.363505 8:+25063794 None:intergenic
AGAGCAGTGTTCAAAGCAAT+TGG 0.364890 8:+25062404 None:intergenic
ACTTAAGCCATGAAGCTAGA+TGG 0.366550 8:-25062568 MsG0880043455.01.T01:CDS
CTTCATATAAACCCTTACTA+TGG 0.380177 8:-25062810 MsG0880043455.01.T01:CDS
TAATGAGGGAACTCATGTTT+GGG 0.380842 8:-25062493 MsG0880043455.01.T01:CDS
GTGAAAATGTACACAGATAA+AGG 0.383039 8:-25062614 MsG0880043455.01.T01:CDS
ATGGCAATATTTCTTTATCC+TGG 0.389498 8:-25063453 MsG0880043455.01.T01:CDS
TGTGAGCAATGAGCATTATT+TGG 0.391167 8:+25062946 None:intergenic
TGTGAATTTGGTGAAGGAAA+TGG 0.399427 8:-25062306 MsG0880043455.01.T01:CDS
ATGAAGCTAGATGGGATTGC+GGG 0.399506 8:-25062559 MsG0880043455.01.T01:CDS
GTTTGATTCAAATGAATTTG+AGG 0.405239 8:+25063925 None:intergenic
ACACTGCTCTTGCTCAACTT+GGG 0.415938 8:-25062391 MsG0880043455.01.T01:CDS
AAAGGTAAAACCGGTGAATT+GGG 0.421134 8:+25063795 None:intergenic
GTGTTCAAAGCAATTGGGCT+TGG 0.432959 8:+25062410 None:intergenic
GTGAATTTGGTGAAGGAAAT+GGG 0.434090 8:-25062305 MsG0880043455.01.T01:CDS
GTACTGTATTTACAGGAAAT+CGG 0.446837 8:-25063144 MsG0880043455.01.T01:intron
GCTCAACGGCCGTAAAAGAT+AGG 0.451675 8:-25063369 MsG0880043455.01.T01:intron
ATAAGCTTTGAATTCAATGT+AGG 0.451843 8:+25062461 None:intergenic
GTTAAAGTTATTGGTAATGC+AGG 0.453786 8:-25062900 MsG0880043455.01.T01:CDS
ATCCTGGAACACATGCTGTA+AGG 0.460244 8:-25063437 MsG0880043455.01.T01:CDS
ACCGAGCGAGGTTTCGCTGT+TGG 0.468409 8:-25062846 MsG0880043455.01.T01:CDS
CAGATTGCAAATGGTGTTGA+AGG 0.470393 8:-25063020 MsG0880043455.01.T01:CDS
ACATATGGCAGCCTGAAAAC+AGG 0.481549 8:+25062359 None:intergenic
CTTAAGCCATGAAGCTAGAT+GGG 0.484577 8:-25062567 MsG0880043455.01.T01:CDS
AGATGGGATTGCGGGGCTGT+TGG 0.489581 8:-25062551 MsG0880043455.01.T01:CDS
GCGAGGTTTCGCTGTTGGAA+TGG 0.491745 8:-25062841 MsG0880043455.01.T01:CDS
ATATAATAGTAACAAGAGAC+AGG 0.495836 8:+25063967 None:intergenic
TCTAAAGTCAGAGGTACATA+TGG 0.496066 8:+25062344 None:intergenic
TTCTAGTATTCCCAATTCAC+CGG 0.500586 8:-25063805 MsG0880043455.01.T01:CDS
CTGCTGGTGAAAGGTAAAAC+CGG 0.500692 8:+25063786 None:intergenic
TATTTACAGGAAATCGGTTG+AGG 0.508091 8:-25063138 MsG0880043455.01.T01:intron
ATTTGGTTCCTGGCTTAATG+AGG 0.508593 8:-25062508 MsG0880043455.01.T01:CDS
GAGCAGTGTTCAAAGCAATT+GGG 0.511149 8:+25062405 None:intergenic
TGACTTTAGAACAGAGGGTA+GGG 0.520952 8:-25062331 MsG0880043455.01.T01:CDS
AGGTAAGTATGGAGTCTTAA+CGG 0.522449 8:+25063088 None:intergenic
TCTTGAAGCATATGATAACT+TGG 0.522761 8:-25063051 MsG0880043455.01.T01:CDS
AAAAGATGTTCAAGTTCTTG+AGG 0.527096 8:-25062261 MsG0880043455.01.T01:CDS
TTGTTAAGTTTAGAATAACT+GGG 0.536064 8:-25063581 MsG0880043455.01.T01:CDS
AATGTTAACCTGCTGGTGAA+AGG 0.537482 8:+25063777 None:intergenic
ATGAATTTGAGGAGAATGAA+AGG 0.537767 8:+25063936 None:intergenic
CACTGCTCTTGCTCAACTTG+GGG 0.541814 8:-25062390 MsG0880043455.01.T01:CDS
AGGGTTTGTGAATTTGGTGA+AGG 0.558889 8:-25062312 MsG0880043455.01.T01:CDS
ATGAGTTCCCTCATTAAGCC+AGG 0.559460 8:+25062500 None:intergenic
CGTTCAGGTCGTATTTGGTG+AGG 0.563918 8:-25064014 MsG0880043455.01.T01:five_prime_UTR
ACCTCTGACTTTAGAACAGA+GGG 0.568160 8:-25062336 MsG0880043455.01.T01:CDS
GTTGCTGGCTCAACTGGTGA+AGG 0.569872 8:-25062990 MsG0880043455.01.T01:CDS
ATTTGGTGAAGGAAATGGGC+AGG 0.570366 8:-25062301 MsG0880043455.01.T01:CDS
TTTGGTTCCTGGCTTAATGA+GGG 0.573703 8:-25062507 MsG0880043455.01.T01:CDS
GTGAAGGCCAATTAATGACA+TGG 0.574512 8:-25062974 MsG0880043455.01.T01:CDS
GTGAACATACAGATTGCAAA+TGG 0.577102 8:-25063029 MsG0880043455.01.T01:CDS
AGATAAAGGAATTGTTGTGT+GGG 0.580604 8:-25062600 MsG0880043455.01.T01:CDS
CTGACTTTAGAACAGAGGGT+AGG 0.581729 8:-25062332 MsG0880043455.01.T01:CDS
AGCAACCAAACCTGCATGTG+AGG 0.584342 8:+25062782 None:intergenic
TGTTAAGTTTAGAATAACTG+GGG 0.585623 8:-25063580 MsG0880043455.01.T01:CDS
GCGATAAACGCAACCGAGCG+AGG 0.588823 8:-25062858 MsG0880043455.01.T01:CDS
TCCAACAGCGAAACCTCGCT+CGG 0.589142 8:+25062845 None:intergenic
GTTGGTGTGAAAGAGTGTGT+GGG 0.596538 8:-25062647 MsG0880043455.01.T01:intron
AATGAGGGAACTCATGTTTG+GGG 0.597288 8:-25062492 MsG0880043455.01.T01:CDS
CAGATAAAGGAATTGTTGTG+TGG 0.598548 8:-25062601 MsG0880043455.01.T01:CDS
TCTCCAGTTGAGCAGCTCAA+CGG 0.599783 8:-25063383 MsG0880043455.01.T01:CDS
TGTTGGTGTGAAAGAGTGTG+TGG 0.601574 8:-25062648 MsG0880043455.01.T01:intron
CATGAAGCTAGATGGGATTG+CGG 0.612299 8:-25062560 MsG0880043455.01.T01:CDS
GTCGTATTTGGTGAGGTGAG+TGG 0.619515 8:-25064007 MsG0880043455.01.T01:five_prime_UTR
GTTAAGTTTAGAATAACTGG+GGG 0.626309 8:-25063579 MsG0880043455.01.T01:CDS
AGAGTGTGTGGGAACTGAGA+GGG 0.629024 8:-25062636 MsG0880043455.01.T01:CDS
GATAAAGGAATTGTTGTGTG+GGG 0.629716 8:-25062599 MsG0880043455.01.T01:CDS
TAAGTTTAGAATAACTGGGG+GGG 0.644203 8:-25063577 MsG0880043455.01.T01:intron
ACCCTCTGTTCTAAAGTCAG+AGG 0.653567 8:+25062335 None:intergenic
ATGAGGGAACTCATGTTTGG+GGG 0.657956 8:-25062491 MsG0880043455.01.T01:CDS
AAGACTCCATACTTACCTAG+TGG 0.662580 8:-25063083 MsG0880043455.01.T01:CDS
TACCTCTGACTTTAGAACAG+AGG 0.672240 8:-25062337 MsG0880043455.01.T01:CDS
TGAAGCTAGATGGGATTGCG+GGG 0.680291 8:-25062558 MsG0880043455.01.T01:CDS
AAGAGTGTGTGGGAACTGAG+AGG 0.690034 8:-25062637 MsG0880043455.01.T01:CDS
TTAAGTTTAGAATAACTGGG+GGG 0.698227 8:-25063578 MsG0880043455.01.T01:CDS
TGAAGGCCAATTAATGACAT+GGG 0.703308 8:-25062973 MsG0880043455.01.T01:CDS
TATAATAGTAACAAGAGACA+GGG 0.727416 8:+25063968 None:intergenic
TAAGTATGGAGTCTTAACGG+CGG 0.771162 8:+25063091 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACATTATTGTATAAATT+TGG + Chr8:25063018-25063037 None:intergenic 10.0%
!! ATGTATCTATCGAATTTAAT+AGG + Chr8:25062955-25062974 None:intergenic 20.0%
!!! AACATCATTTAAGATAAGAA+TGG + Chr8:25062574-25062593 None:intergenic 20.0%
!!! AATTTTGCTTAGTTTAATGA+TGG - Chr8:25062778-25062797 MsG0880043455.01.T01:intron 20.0%
!!! ATTATCTTTTTAAGCTTTTG+TGG - Chr8:25063062-25063081 MsG0880043455.01.T01:CDS 20.0%
!!! TTGTTAAGTTTAGAATAACT+GGG - Chr8:25062669-25062688 MsG0880043455.01.T01:intron 20.0%
!!! TTTGTTAAGTTTAGAATAAC+TGG - Chr8:25062668-25062687 MsG0880043455.01.T01:intron 20.0%
! AAAAACCATGGATCATAAAT+AGG - Chr8:25062645-25062664 MsG0880043455.01.T01:intron 25.0%
! AATATTTCAATTACACTAGG+AGG + Chr8:25063564-25063583 None:intergenic 25.0%
! ATATAATAGTAACAAGAGAC+AGG + Chr8:25062286-25062305 None:intergenic 25.0%
! GTTTGATTCAAATGAATTTG+AGG + Chr8:25062328-25062347 None:intergenic 25.0%
! TATAATAGTAACAAGAGACA+GGG + Chr8:25062285-25062304 None:intergenic 25.0%
! TCCTAGTGTAATTGAAATAT+TGG - Chr8:25063563-25063582 MsG0880043455.01.T01:intron 25.0%
!! CAAATCAAACCTATCTTTTA+CGG + Chr8:25062893-25062912 None:intergenic 25.0%
!! GATGATGATTTTATCATAGT+TGG - Chr8:25064011-25064030 MsG0880043455.01.T01:five_prime_UTR 25.0%
!! TGTTAAGTTTAGAATAACTG+GGG - Chr8:25062670-25062689 MsG0880043455.01.T01:intron 25.0%
!! TTTTAAGCTTTTGTGGTAAT+GGG - Chr8:25063069-25063088 MsG0880043455.01.T01:CDS 25.0%
!!! ATAAGCTTTGAATTCAATGT+AGG + Chr8:25063792-25063811 None:intergenic 25.0%
!!! TATAATAGTTTGCTTCCTAT+AGG - Chr8:25063494-25063513 MsG0880043455.01.T01:intron 25.0%
!!! TTTTTAAGAGCTTCAATTGA+TGG - Chr8:25062351-25062370 MsG0880043455.01.T01:CDS 25.0%
!!! TTTTTAAGCTTTTGTGGTAA+TGG - Chr8:25063068-25063087 MsG0880043455.01.T01:CDS 25.0%
AAGAAAAAATGTTAACCTGC+TGG + Chr8:25062483-25062502 None:intergenic 30.0%
AATTCGATAGATACATTTGC+AGG - Chr8:25062959-25062978 MsG0880043455.01.T01:CDS 30.0%
ACAAACCTATTTATGATCCA+TGG + Chr8:25062653-25062672 None:intergenic 30.0%
AGATAAAGGAATTGTTGTGT+GGG - Chr8:25063650-25063669 MsG0880043455.01.T01:intron 30.0%
AGGTACATTGTATATGATGA+TGG + Chr8:25063521-25063540 None:intergenic 30.0%
ATCATATACAATGTACCTCA+AGG - Chr8:25063523-25063542 MsG0880043455.01.T01:intron 30.0%
ATGAATTTGAGGAGAATGAA+AGG + Chr8:25062317-25062336 None:intergenic 30.0%
ATGGCAATATTTCTTTATCC+TGG - Chr8:25062797-25062816 MsG0880043455.01.T01:CDS 30.0%
CATATGTTGTGTAAAAACCA+TGG - Chr8:25062633-25062652 MsG0880043455.01.T01:CDS 30.0%
CTTCATATAAACCCTTACTA+TGG - Chr8:25063440-25063459 MsG0880043455.01.T01:CDS 30.0%
GCCAATATTTCAATTACACT+AGG + Chr8:25063567-25063586 None:intergenic 30.0%
GGTACATTGTATATGATGAT+GGG + Chr8:25063520-25063539 None:intergenic 30.0%
GTACTGTATTTACAGGAAAT+CGG - Chr8:25063106-25063125 MsG0880043455.01.T01:CDS 30.0%
GTGAAAATGTACACAGATAA+AGG - Chr8:25063636-25063655 MsG0880043455.01.T01:intron 30.0%
TCTTGAAGCATATGATAACT+TGG - Chr8:25063199-25063218 MsG0880043455.01.T01:intron 30.0%
TCTTTCACACCAACAAAATT+TGG + Chr8:25063597-25063616 None:intergenic 30.0%
TTTCTGTGTACTGTATTTAC+AGG - Chr8:25063099-25063118 MsG0880043455.01.T01:CDS 30.0%
! AAAAGATGTTCAAGTTCTTG+AGG - Chr8:25063989-25064008 MsG0880043455.01.T01:five_prime_UTR 30.0%
! AGCTTATGACTTTGTTTGAT+TGG - Chr8:25063805-25063824 MsG0880043455.01.T01:CDS 30.0%
! ATTTTTCCACTAGGTAAGTA+TGG + Chr8:25063176-25063195 None:intergenic 30.0%
! CATGGTTTTTACACAACATA+TGG + Chr8:25062635-25062654 None:intergenic 30.0%
! GCTCAAAATCCAAATTTTGT+TGG - Chr8:25063585-25063604 MsG0880043455.01.T01:CDS 30.0%
! GTTAAGTTTAGAATAACTGG+GGG - Chr8:25062671-25062690 MsG0880043455.01.T01:intron 30.0%
! TTAAGTTTAGAATAACTGGG+GGG - Chr8:25062672-25062691 MsG0880043455.01.T01:intron 30.0%
!! GTTAAAGTTATTGGTAATGC+AGG - Chr8:25063350-25063369 MsG0880043455.01.T01:intron 30.0%
!! TCAAGATCTATTTTTCCACT+AGG + Chr8:25063185-25063204 None:intergenic 30.0%
AAAGGTAAAACCGGTGAATT+GGG + Chr8:25062458-25062477 None:intergenic 35.0%
AGGTAAGTATGGAGTCTTAA+CGG + Chr8:25063165-25063184 None:intergenic 35.0%
CAGATAAAGGAATTGTTGTG+TGG - Chr8:25063649-25063668 MsG0880043455.01.T01:intron 35.0%
GATAAAGGAATTGTTGTGTG+GGG - Chr8:25063651-25063670 MsG0880043455.01.T01:intron 35.0%
GGCGACAAAGTTAAAGTTAT+TGG - Chr8:25063341-25063360 MsG0880043455.01.T01:intron 35.0%
GTGAACATACAGATTGCAAA+TGG - Chr8:25063221-25063240 MsG0880043455.01.T01:intron 35.0%
TAATGAGGGAACTCATGTTT+GGG - Chr8:25063757-25063776 MsG0880043455.01.T01:intron 35.0%
TATTTACAGGAAATCGGTTG+AGG - Chr8:25063112-25063131 MsG0880043455.01.T01:CDS 35.0%
TCAATCTGTTGCAAGCAATT+TGG - Chr8:25063725-25063744 MsG0880043455.01.T01:intron 35.0%
TGAAGGCCAATTAATGACAT+GGG - Chr8:25063277-25063296 MsG0880043455.01.T01:intron 35.0%
TGTGAGCAATGAGCATTATT+TGG + Chr8:25063307-25063326 None:intergenic 35.0%
TTAATGAGGGAACTCATGTT+TGG - Chr8:25063756-25063775 MsG0880043455.01.T01:intron 35.0%
TTCTAGTATTCCCAATTCAC+CGG - Chr8:25062445-25062464 MsG0880043455.01.T01:CDS 35.0%
! AGAAGTCTAAGACAAGTCAT+TGG - Chr8:25062703-25062722 MsG0880043455.01.T01:intron 35.0%
! GCTCACACAGTTAATTGTTT+TGG - Chr8:25063320-25063339 MsG0880043455.01.T01:intron 35.0%
! GTGAATTTGGTGAAGGAAAT+GGG - Chr8:25063945-25063964 MsG0880043455.01.T01:five_prime_UTR 35.0%
! GTGAGGTTTTTCCATAGTAA+GGG + Chr8:25063454-25063473 None:intergenic 35.0%
! TAAGTTTAGAATAACTGGGG+GGG - Chr8:25062673-25062692 MsG0880043455.01.T01:intron 35.0%
! TCTAAAGTCAGAGGTACATA+TGG + Chr8:25063909-25063928 None:intergenic 35.0%
! TGTGAATTTGGTGAAGGAAA+TGG - Chr8:25063944-25063963 MsG0880043455.01.T01:five_prime_UTR 35.0%
! TGTGAGGTTTTTCCATAGTA+AGG + Chr8:25063455-25063474 None:intergenic 35.0%
AAAAACCTCACATGCAGGTT+TGG - Chr8:25063463-25063482 MsG0880043455.01.T01:CDS 40.0%
AAGACTCCATACTTACCTAG+TGG - Chr8:25063167-25063186 MsG0880043455.01.T01:intron 40.0%
AATATCTTGACCACTCCTTG+AGG + Chr8:25063541-25063560 None:intergenic 40.0%
AATGAGGGAACTCATGTTTG+GGG - Chr8:25063758-25063777 MsG0880043455.01.T01:intron 40.0%
AATGTTAACCTGCTGGTGAA+AGG + Chr8:25062476-25062495 None:intergenic 40.0%
ACTCTGCAGTTTATGCTGTT+TGG - Chr8:25062532-25062551 MsG0880043455.01.T01:CDS 40.0%
ACTTAAGCCATGAAGCTAGA+TGG - Chr8:25063682-25063701 MsG0880043455.01.T01:intron 40.0%
AGAGCAGTGTTCAAAGCAAT+TGG + Chr8:25063849-25063868 None:intergenic 40.0%
ATACAATGTACCTCAAGGAG+TGG - Chr8:25063528-25063547 MsG0880043455.01.T01:intron 40.0%
CTTAAGCCATGAAGCTAGAT+GGG - Chr8:25063683-25063702 MsG0880043455.01.T01:intron 40.0%
GAAAGGTAAAACCGGTGAAT+TGG + Chr8:25062459-25062478 None:intergenic 40.0%
GAGCAGTGTTCAAAGCAATT+GGG + Chr8:25063848-25063867 None:intergenic 40.0%
GATAGATACATTTGCAGGAC+TGG - Chr8:25062964-25062983 MsG0880043455.01.T01:CDS 40.0%
GGTCATCCCATGTCATTAAT+TGG + Chr8:25063286-25063305 None:intergenic 40.0%
GTGAAGGCCAATTAATGACA+TGG - Chr8:25063276-25063295 MsG0880043455.01.T01:intron 40.0%
TAAGTATGGAGTCTTAACGG+CGG + Chr8:25063162-25063181 None:intergenic 40.0%
TATGGAAAAACCTCACATGC+AGG - Chr8:25063458-25063477 MsG0880043455.01.T01:CDS 40.0%
TCATTCGTTCAGGTCGTATT+TGG - Chr8:25062231-25062250 MsG0880043455.01.T01:CDS 40.0%
! AGGGTTTGTGAATTTGGTGA+AGG - Chr8:25063938-25063957 MsG0880043455.01.T01:five_prime_UTR 40.0%
! ATGGGCAGGCAACATTTTAT+TGG - Chr8:25063963-25063982 MsG0880043455.01.T01:five_prime_UTR 40.0%
! ATTTGGTTCCTGGCTTAATG+AGG - Chr8:25063742-25063761 MsG0880043455.01.T01:intron 40.0%
! CAGATTGCAAATGGTGTTGA+AGG - Chr8:25063230-25063249 MsG0880043455.01.T01:intron 40.0%
! TTTGGTTCCTGGCTTAATGA+GGG - Chr8:25063743-25063762 MsG0880043455.01.T01:intron 40.0%
!! ACCTCTGACTTTAGAACAGA+GGG - Chr8:25063914-25063933 MsG0880043455.01.T01:five_prime_UTR 40.0%
!! GTATATGATGATGGGCCTAT+AGG + Chr8:25063512-25063531 None:intergenic 40.0%
!! GTTGAAGGTATTCTTGTTGC+TGG - Chr8:25063245-25063264 MsG0880043455.01.T01:intron 40.0%
!! TACCTCTGACTTTAGAACAG+AGG - Chr8:25063913-25063932 MsG0880043455.01.T01:five_prime_UTR 40.0%
!! TGACTTTAGAACAGAGGGTA+GGG - Chr8:25063919-25063938 MsG0880043455.01.T01:five_prime_UTR 40.0%
AACACTGCTCTTGCTCAACT+TGG - Chr8:25063858-25063877 MsG0880043455.01.T01:five_prime_UTR 45.0%
AACCTTACAGCATGTGTTCC+AGG + Chr8:25062818-25062837 None:intergenic 45.0%
ACACTGCTCTTGCTCAACTT+GGG - Chr8:25063859-25063878 MsG0880043455.01.T01:five_prime_UTR 45.0%
ACATATGGCAGCCTGAAAAC+AGG + Chr8:25063894-25063913 None:intergenic 45.0%
ATCCTGGAACACATGCTGTA+AGG - Chr8:25062813-25062832 MsG0880043455.01.T01:CDS 45.0%
ATGAAGCTAGATGGGATTGC+GGG - Chr8:25063691-25063710 MsG0880043455.01.T01:intron 45.0%
ATGAGGGAACTCATGTTTGG+GGG - Chr8:25063759-25063778 MsG0880043455.01.T01:intron 45.0%
ATGAGTTCCCTCATTAAGCC+AGG + Chr8:25063753-25063772 None:intergenic 45.0%
CATGAAGCTAGATGGGATTG+CGG - Chr8:25063690-25063709 MsG0880043455.01.T01:intron 45.0%
CTGCTGGTGAAAGGTAAAAC+CGG + Chr8:25062467-25062486 None:intergenic 45.0%
GAGGGTAGGGTTTGTGAATT+TGG - Chr8:25063932-25063951 MsG0880043455.01.T01:five_prime_UTR 45.0%
GTGTTCAAAGCAATTGGGCT+TGG + Chr8:25063843-25063862 None:intergenic 45.0%
! ATTTGGTGAAGGAAATGGGC+AGG - Chr8:25063949-25063968 MsG0880043455.01.T01:five_prime_UTR 45.0%
! GGGTTATCAAGCCTGTTTTC+AGG - Chr8:25063880-25063899 MsG0880043455.01.T01:five_prime_UTR 45.0%
! GTTGCAAGCAATTTGGTTCC+TGG - Chr8:25063732-25063751 MsG0880043455.01.T01:intron 45.0%
! GTTGGTGTGAAAGAGTGTGT+GGG - Chr8:25063603-25063622 MsG0880043455.01.T01:intron 45.0%
! TGTTGGTGTGAAAGAGTGTG+TGG - Chr8:25063602-25063621 MsG0880043455.01.T01:intron 45.0%
!! ACCCTCTGTTCTAAAGTCAG+AGG + Chr8:25063918-25063937 None:intergenic 45.0%
!! ATTCTTGTTGCTGGCTCAAC+TGG - Chr8:25063254-25063273 MsG0880043455.01.T01:intron 45.0%
!! CTGACTTTAGAACAGAGGGT+AGG - Chr8:25063918-25063937 MsG0880043455.01.T01:five_prime_UTR 45.0%
AGAGTGTGTGGGAACTGAGA+GGG - Chr8:25063614-25063633 MsG0880043455.01.T01:intron 50.0%
AGCAACCAAACCTGCATGTG+AGG + Chr8:25063471-25063490 None:intergenic 50.0%
CACTGCTCTTGCTCAACTTG+GGG - Chr8:25063860-25063879 MsG0880043455.01.T01:five_prime_UTR 50.0%
GCTCAACGGCCGTAAAAGAT+AGG - Chr8:25062881-25062900 MsG0880043455.01.T01:CDS 50.0%
TCTCCAGTTGAGCAGCTCAA+CGG - Chr8:25062867-25062886 MsG0880043455.01.T01:CDS 50.0%
TGAAGCTAGATGGGATTGCG+GGG - Chr8:25063692-25063711 MsG0880043455.01.T01:intron 50.0%
! AAGAGTGTGTGGGAACTGAG+AGG - Chr8:25063613-25063632 MsG0880043455.01.T01:intron 50.0%
! CGCAATCCCATCTAGCTTCA+TGG + Chr8:25063692-25063711 None:intergenic 50.0%
! CGGTTTTACCTTTCACCAGC+AGG - Chr8:25062465-25062484 MsG0880043455.01.T01:CDS 50.0%
! CGTTCAGGTCGTATTTGGTG+AGG - Chr8:25062236-25062255 MsG0880043455.01.T01:CDS 50.0%
! GTCGTATTTGGTGAGGTGAG+TGG - Chr8:25062243-25062262 MsG0880043455.01.T01:CDS 50.0%
GCGAGGTTTCGCTGTTGGAA+TGG - Chr8:25063409-25063428 MsG0880043455.01.T01:CDS 55.0%
TCCAACAGCGAAACCTCGCT+CGG + Chr8:25063408-25063427 None:intergenic 55.0%
!! GTTGCTGGCTCAACTGGTGA+AGG - Chr8:25063260-25063279 MsG0880043455.01.T01:intron 55.0%
ACCGAGCGAGGTTTCGCTGT+TGG - Chr8:25063404-25063423 MsG0880043455.01.T01:CDS 60.0%
AGATGGGATTGCGGGGCTGT+TGG - Chr8:25063699-25063718 MsG0880043455.01.T01:intron 60.0%
GCGATAAACGCAACCGAGCG+AGG - Chr8:25063392-25063411 MsG0880043455.01.T01:CDS 60.0%
CGGCCGTTGAGCTGCTCAAC+TGG + Chr8:25062873-25062892 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 25062229 25064043 25062229 ID=MsG0880043455.01;Name=MsG0880043455.01
Chr8 mRNA 25062229 25064043 25062229 ID=MsG0880043455.01.T01;Parent=MsG0880043455.01;Name=MsG0880043455.01.T01;_AED=0.45;_eAED=0.48;_QI=196|0|0.2|1|0.5|0.4|5|0|329
Chr8 exon 25062229 25062656 25062229 ID=MsG0880043455.01.T01:exon:1103;Parent=MsG0880043455.01.T01
Chr8 exon 25062793 25063151 25062793 ID=MsG0880043455.01.T01:exon:1102;Parent=MsG0880043455.01.T01
Chr8 exon 25063370 25063482 25063370 ID=MsG0880043455.01.T01:exon:1101;Parent=MsG0880043455.01.T01
Chr8 exon 25063578 25063605 25063578 ID=MsG0880043455.01.T01:exon:1100;Parent=MsG0880043455.01.T01
Chr8 exon 25063786 25064043 25063786 ID=MsG0880043455.01.T01:exon:1099;Parent=MsG0880043455.01.T01
Chr8 five_prime_UTR 25063848 25064043 25063848 ID=MsG0880043455.01.T01:five_prime_utr;Parent=MsG0880043455.01.T01
Chr8 CDS 25063786 25063847 25063786 ID=MsG0880043455.01.T01:cds;Parent=MsG0880043455.01.T01
Chr8 CDS 25063578 25063605 25063578 ID=MsG0880043455.01.T01:cds;Parent=MsG0880043455.01.T01
Chr8 CDS 25063370 25063482 25063370 ID=MsG0880043455.01.T01:cds;Parent=MsG0880043455.01.T01
Chr8 CDS 25062793 25063151 25062793 ID=MsG0880043455.01.T01:cds;Parent=MsG0880043455.01.T01
Chr8 CDS 25062229 25062656 25062229 ID=MsG0880043455.01.T01:cds;Parent=MsG0880043455.01.T01
Gene Sequence

>MsG0880043455.01.T01

ATGAACATGTTGAAAACTGATTCTAGTATTCCCAATTCACCGGTTTTACCTTTCACCAGCAGGTTTGTTAAGTTTAGAATAACTGGGGGGTTTAATGATGGCAATATTTCTTTATCCTGGAACACATGCTGTAAGGTTCAACAAACAGCTGCGAGACTTGATGTTCGTCTCCAGTTGAGCAGCTCAACGGCCGTAAAAGATAGGAAATCGGTTGAGGATGTACGAAGTCTACGATTCATCACCGCCGTTAAGACTCCATACTTACCTAGTGGAAAAATAGATCTTGAAGCATATGATAACTTGGTGAACATACAGATTGCAAATGGTGTTGAAGGTATTCTTGTTGCTGGCTCAACTGGTGAAGGCCAATTAATGACATGGGATGACCAAATAATGCTCATTGCTCACACAGTTAATTGTTTTGGCGACAAAGTTAAAGTTATTGGTAATGCAGGAAGCAATTGCACAAAAGAAGCGATAAACGCAACCGAGCGAGGTTTCGCTGTTGGAATGGATGCTGCACTTCATATAAACCCTTACTATGGAAAAACCTCACATGCAGAGTGTGTGGGAACTGAGAGGGTGAAAATGTACACAGATAAAGGAATTGTTGTGTGGGGAATTGATAACTTAAGCCATGAAGCTAGATGGGATTGCGGGGCTGTTGGAGTTCAATCTGTTGCAAGCAATTTGGTTCCTGGCTTAATGAGGGAACTCATGTTTGGGGGTAAGAATCCTACATTGAATTCAAAGCTTATGACTTTGTTTGATTGGCTTTTTATAGAGCCAAGCCCAATTGCTTTGAACACTGCTCTTGCTCAACTTGGGGTTATCAAGCCTGTTTTCAGGCTGCCATATGTACCTCTGACTTTAGAACAGAGGGTAGGGTTTGTGAATTTGGTGAAGGAAATGGGCAGGCAACATTTTATTGGAGAAAAAGATGTTCAAGTTCTTGAGGATGATGATTTTATCATAGTTGGTCGATATTAA
Protein sequence

>MsG0880043455.01.T01

MNMLKTDSSIPNSPVLPFTSRFVKFRITGGFNDGNISLSWNTCCKVQQTAARLDVRLQLSSSTAVKDRKSVEDVRSLRFITAVKTPYLPSGKIDLEAYDNLVNIQIANGVEGILVAGSTGEGQLMTWDDQIMLIAHTVNCFGDKVKVIGNAGSNCTKEAINATERGFAVGMDAALHINPYYGKTSHAECVGTERVKMYTDKGIVVWGIDNLSHEARWDCGAVGVQSVASNLVPGLMRELMFGGKNPTLNSKLMTLFDWLFIEPSPIALNTALAQLGVIKPVFRLPYVPLTLEQRVGFVNLVKEMGRQHFIGEKDVQVLEDDDFIIVGRY*