AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043802.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043802.01.T01 MTR_5g037680 44.220 346 140 11 1 303 45 380 2.13e-76 241
MsG0880043802.01.T01 MTR_5g059430 40.054 367 149 13 1 304 63 421 7.19e-72 228
MsG0880043802.01.T01 MTR_5g069390 41.525 354 157 12 1 306 63 414 3.06e-71 228
MsG0880043802.01.T01 MTR_6g086825 40.000 360 151 13 1 305 107 456 7.57e-71 226
MsG0880043802.01.T01 MTR_0129s0070 40.698 344 162 11 1 306 54 393 1.19e-69 222
MsG0880043802.01.T01 MTR_5g097330 39.045 356 157 11 1 305 53 399 3.39e-68 218
MsG0880043802.01.T01 MTR_5g096910 45.161 310 126 11 33 306 89 390 4.84e-68 217
MsG0880043802.01.T01 MTR_2g038680 40.771 363 151 13 1 306 53 408 2.05e-67 216
MsG0880043802.01.T01 MTR_8g012090 43.871 310 116 10 1 280 53 334 2.82e-67 213
MsG0880043802.01.T01 MTR_0129s0120 40.816 343 161 11 1 305 54 392 4.44e-67 215
MsG0880043802.01.T01 MTR_5g086590 48.684 228 110 4 59 283 8 231 2.71e-66 207
MsG0880043802.01.T01 MTR_6g080680 38.596 342 163 11 1 303 56 389 7.32e-66 212
MsG0880043802.01.T01 MTR_2g022000 38.056 360 167 8 1 305 53 411 3.55e-64 208
MsG0880043802.01.T01 MTR_2g021800 38.095 357 164 8 1 301 53 408 1.10e-62 204
MsG0880043802.01.T01 MTR_8g063440 39.466 337 168 10 1 306 49 380 5.00e-62 201
MsG0880043802.01.T01 MTR_7g017040 39.031 351 161 12 1 306 53 395 6.06e-62 202
MsG0880043802.01.T01 MTR_5g097350 38.643 339 164 10 2 305 54 383 6.45e-62 201
MsG0880043802.01.T01 MTR_5g064700 38.544 371 155 16 3 306 1 365 8.76e-62 200
MsG0880043802.01.T01 MTR_4g130330 39.244 344 160 9 8 306 49 388 6.07e-61 199
MsG0880043802.01.T01 MTR_6g478230 38.936 357 147 14 1 304 64 402 1.23e-60 199
MsG0880043802.01.T01 MTR_4g028660 39.024 328 158 8 1 289 53 377 1.67e-60 198
MsG0880043802.01.T01 MTR_4g027450 39.024 328 158 8 1 289 53 377 1.67e-60 198
MsG0880043802.01.T01 MTR_4g028510 37.572 346 176 9 1 306 54 399 1.89e-60 199
MsG0880043802.01.T01 MTR_3g011120 39.297 313 157 10 5 291 55 360 1.99e-60 197
MsG0880043802.01.T01 MTR_5g096900 47.955 269 99 7 1 233 49 312 6.63e-60 194
MsG0880043802.01.T01 MTR_7g017130 36.676 349 169 12 1 306 55 394 9.70e-60 196
MsG0880043802.01.T01 MTR_6g079810 38.567 363 157 15 1 306 107 460 3.97e-59 196
MsG0880043802.01.T01 MTR_6g090180 36.827 353 173 11 1 305 84 434 5.63e-59 195
MsG0880043802.01.T01 MTR_7g017170 37.615 327 154 11 1 286 55 372 6.67e-59 194
MsG0880043802.01.T01 MTR_5g097220 38.356 365 155 13 1 305 50 404 6.94e-59 194
MsG0880043802.01.T01 MTR_5g097850 57.143 182 67 4 134 306 63 242 1.45e-58 188
MsG0880043802.01.T01 MTR_5g092860 39.688 320 149 10 1 286 39 348 5.71e-58 190
MsG0880043802.01.T01 MTR_6g079770 38.356 365 162 14 1 306 54 414 9.89e-58 191
MsG0880043802.01.T01 MTR_4g028680 38.040 347 172 10 1 306 53 397 1.13e-57 191
MsG0880043802.01.T01 MTR_6g461660 39.595 346 163 9 1 305 56 396 1.56e-57 190
MsG0880043802.01.T01 MTR_8g464770 37.309 327 170 9 6 303 54 374 3.10e-57 189
MsG0880043802.01.T01 MTR_0054s0090 38.068 352 168 13 1 305 54 402 4.09e-57 189
MsG0880043802.01.T01 MTR_5g097680 37.135 377 160 15 1 306 54 424 6.23e-57 189
MsG0880043802.01.T01 MTR_0054s0100 38.393 336 163 12 1 292 54 389 1.12e-56 188
MsG0880043802.01.T01 MTR_2g104340 40.058 347 159 14 5 306 51 393 1.55e-56 187
MsG0880043802.01.T01 MTR_2g021770 37.293 362 163 12 1 305 49 403 1.67e-56 188
MsG0880043802.01.T01 MTR_2g021950 36.550 342 179 9 1 305 53 393 3.93e-55 184
MsG0880043802.01.T01 MTR_7g079640 39.617 313 151 12 1 286 50 351 7.29e-55 183
MsG0880043802.01.T01 MTR_4g055820 40.777 309 139 9 1 266 51 358 8.69e-55 182
MsG0880043802.01.T01 MTR_6g078010 37.500 344 161 11 1 304 1 330 2.15e-54 181
MsG0880043802.01.T01 MTR_0129s0050 36.389 360 170 12 1 306 51 405 2.59e-54 182
MsG0880043802.01.T01 MTR_7g111700 38.218 348 139 12 7 306 43 362 1.14e-53 179
MsG0880043802.01.T01 MTR_6g090250 35.733 375 167 12 1 304 48 419 1.14e-53 181
MsG0880043802.01.T01 MTR_8g041270 37.960 353 167 10 5 306 52 403 2.05e-53 180
MsG0880043802.01.T01 MTR_4g007940 35.650 331 164 10 14 299 4 330 2.50e-53 177
MsG0880043802.01.T01 MTR_4g028750 36.086 327 162 10 1 286 53 373 4.44e-53 179
MsG0880043802.01.T01 MTR_2g104280 37.288 354 168 12 5 306 51 402 5.21e-53 179
MsG0880043802.01.T01 MTR_6g079790 39.130 276 137 10 60 306 33 306 7.66e-53 176
MsG0880043802.01.T01 MTR_5g096630 36.415 357 174 10 1 305 55 410 3.16e-52 177
MsG0880043802.01.T01 MTR_7g069550 35.882 340 165 10 1 294 51 383 7.74e-52 175
MsG0880043802.01.T01 MTR_4g117550 35.447 347 153 13 1 306 54 370 9.72e-52 174
MsG0880043802.01.T01 MTR_5g074800 36.223 323 164 8 3 286 1 320 1.37e-51 173
MsG0880043802.01.T01 MTR_1g007450 38.739 333 163 10 1 294 49 379 2.76e-51 174
MsG0880043802.01.T01 MTR_2g064950 37.538 333 163 11 1 294 47 373 7.82e-51 172
MsG0880043802.01.T01 MTR_0831s0010 33.929 336 188 10 1 306 87 418 1.64e-50 172
MsG0880043802.01.T01 MTR_0134s0060 35.574 357 159 14 1 295 49 396 1.55e-49 170
MsG0880043802.01.T01 MTR_4g102410 36.000 350 178 12 1 305 98 446 4.59e-49 169
MsG0880043802.01.T01 MTR_6g090260 45.226 199 98 4 116 304 62 259 7.12e-49 164
MsG0880043802.01.T01 MTR_1g007430 39.016 305 137 9 1 269 33 324 9.41e-49 165
MsG0880043802.01.T01 MTR_2g066170 35.191 341 180 13 1 306 49 383 1.86e-48 166
MsG0880043802.01.T01 MTR_5g074850 37.574 338 157 15 1 291 74 404 2.21e-48 173
MsG0880043802.01.T01 MTR_1g007410 37.647 340 151 13 1 291 59 386 3.30e-48 166
MsG0880043802.01.T01 MTR_5g075370 37.168 339 160 15 1 291 47 380 3.53e-48 172
MsG0880043802.01.T01 MTR_6g087620 36.396 283 125 9 33 283 1 260 1.48e-47 161
MsG0880043802.01.T01 MTR_5g097540 40.288 278 112 9 1 269 1 233 2.58e-47 161
MsG0880043802.01.T01 MTR_2g065840 33.138 341 190 9 1 306 49 386 6.37e-47 162
MsG0880043802.01.T01 MTR_2g065860 34.024 338 190 10 1 306 49 385 7.00e-47 162
MsG0880043802.01.T01 MTR_2g034700 36.050 319 165 10 1 292 51 357 2.78e-46 160
MsG0880043802.01.T01 MTR_5g091140 37.061 313 162 11 1 285 85 390 3.73e-46 161
MsG0880043802.01.T01 MTR_8g039500 37.097 310 153 12 1 280 49 346 1.10e-45 158
MsG0880043802.01.T01 MTR_5g076490 38.109 349 157 15 1 306 1 333 1.41e-45 157
MsG0880043802.01.T01 MTR_8g036970 34.591 318 133 10 1 291 35 304 1.43e-45 158
MsG0880043802.01.T01 MTR_8g046220 35.068 365 161 17 1 305 54 402 4.83e-45 158
MsG0880043802.01.T01 MTR_8g046310 35.484 341 163 14 1 304 53 373 1.12e-44 156
MsG0880043802.01.T01 MTR_2g066200 34.783 345 176 16 1 305 49 384 1.26e-44 156
MsG0880043802.01.T01 MTR_8g013000 38.065 310 148 10 1 269 47 353 1.75e-44 156
MsG0880043802.01.T01 MTR_7g023800 35.890 326 143 12 3 294 1 294 2.21e-44 154
MsG0880043802.01.T01 MTR_5g023970 44.324 185 94 5 128 305 10 192 2.22e-44 150
MsG0880043802.01.T01 MTR_5g092850 33.616 354 178 14 1 305 53 398 1.16e-43 154
MsG0880043802.01.T01 MTR_6g004450 41.322 242 112 9 87 305 31 265 1.38e-43 150
MsG0880043802.01.T01 MTR_5g072060 36.570 309 169 7 1 286 43 347 1.59e-43 157
MsG0880043802.01.T01 MTR_8g467670 32.063 315 172 10 7 291 57 359 1.73e-43 153
MsG0880043802.01.T01 MTR_2g066150 32.931 331 173 12 1 305 49 356 3.83e-43 152
MsG0880043802.01.T01 MTR_8g064890 33.815 346 176 12 1 306 53 385 4.10e-43 152
MsG0880043802.01.T01 MTR_2g021920 40.426 235 116 5 95 305 35 269 1.05e-42 148
MsG0880043802.01.T01 MTR_8g063330 33.224 304 159 10 1 266 51 348 3.18e-42 152
MsG0880043802.01.T01 MTR_5g022850 36.593 317 151 15 33 306 41 350 4.44e-42 149
MsG0880043802.01.T01 MTR_4g014370 33.425 365 176 14 1 305 48 405 1.01e-41 149
MsG0880043802.01.T01 MTR_5g024260 33.435 329 178 11 1 290 59 385 1.16e-41 149
MsG0880043802.01.T01 MTR_8g033140 34.435 363 155 15 1 296 56 402 2.82e-41 148
MsG0880043802.01.T01 MTR_8g046200 33.623 345 152 12 30 304 80 417 3.08e-41 148
MsG0880043802.01.T01 MTR_5g043605 37.109 256 129 10 67 306 97 336 1.29e-40 145
MsG0880043802.01.T01 MTR_1g053510 33.056 360 175 14 1 304 52 401 5.75e-40 144
MsG0880043802.01.T01 MTR_1g098180 32.722 327 176 12 3 306 58 363 2.37e-39 142
MsG0880043802.01.T01 MTR_5g096920 50.331 151 70 3 159 306 1 149 2.89e-39 135
MsG0880043802.01.T01 MTR_7g016930 37.500 272 120 10 1 242 53 304 5.31e-39 140
MsG0880043802.01.T01 MTR_8g033090 32.768 354 171 10 1 295 51 396 2.79e-38 140
MsG0880043802.01.T01 MTR_8g063370 31.915 329 160 15 1 287 61 367 7.75e-38 138
MsG0880043802.01.T01 MTR_8g063350 31.338 284 159 11 33 287 45 321 2.35e-37 136
MsG0880043802.01.T01 MTR_8g033000 30.239 377 175 10 1 306 1 360 3.29e-37 136
MsG0880043802.01.T01 MTR_1g050312 33.555 301 139 11 30 299 13 283 7.83e-37 133
MsG0880043802.01.T01 MTR_5g066420 46.309 149 71 3 159 298 1 149 1.79e-36 128
MsG0880043802.01.T01 MTR_5g068490 31.724 290 150 10 42 306 8 274 3.88e-36 131
MsG0880043802.01.T01 MTR_8g032980 31.680 363 162 12 1 291 53 401 5.48e-36 134
MsG0880043802.01.T01 MTR_8g033110 31.880 367 178 14 1 303 51 409 8.60e-36 133
MsG0880043802.01.T01 MTR_4g014370 32.188 320 157 12 1 305 59 333 4.36e-35 130
MsG0880043802.01.T01 MTR_6g079730 50.000 132 64 1 175 304 14 145 5.00e-35 124
MsG0880043802.01.T01 MTR_8g035690 32.852 277 140 10 70 306 30 300 6.27e-33 123
MsG0880043802.01.T01 MTR_8g018110 30.548 347 173 11 1 295 51 381 1.23e-32 124
MsG0880043802.01.T01 MTR_6g009350 41.270 189 89 7 101 273 12 194 3.30e-32 118
MsG0880043802.01.T01 MTR_3g018750 35.714 238 113 9 101 305 7 237 1.05e-31 118
MsG0880043802.01.T01 MTR_7g017120 43.103 174 87 5 120 286 72 240 3.06e-31 118
MsG0880043802.01.T01 MTR_7g017150 43.103 174 87 5 120 286 73 241 3.74e-31 117
MsG0880043802.01.T01 MTR_5g098950 36.019 211 102 3 66 269 90 274 1.24e-30 116
MsG0880043802.01.T01 MTR_7g104710 35.271 258 110 8 1 226 54 286 1.67e-30 117
MsG0880043802.01.T01 MTR_8g064790 28.955 335 157 12 1 306 52 334 2.68e-30 117
MsG0880043802.01.T01 MTR_1g050308 30.931 333 144 10 1 306 71 344 2.85e-30 117
MsG0880043802.01.T01 MTR_4g016000 35.749 207 101 8 69 261 2 190 5.06e-30 113
MsG0880043802.01.T01 MTR_7g019250 33.036 224 121 6 78 288 206 413 4.49e-28 113
MsG0880043802.01.T01 MTR_5g097820 35.211 213 101 9 1 179 60 269 3.71e-27 107
MsG0880043802.01.T01 MTR_5g097660 50.943 106 49 2 203 305 11 116 3.76e-27 103
MsG0880043802.01.T01 MTR_5g096050 57.407 108 33 2 159 253 1 108 4.66e-27 102
MsG0880043802.01.T01 MTR_5g086780 51.429 105 51 0 182 286 123 227 2.19e-26 104
MsG0880043802.01.T01 MTR_3g030620 38.182 165 91 4 152 306 16 179 6.10e-26 101
MsG0880043802.01.T01 MTR_8g063310 27.273 319 188 13 6 286 60 372 1.25e-25 105
MsG0880043802.01.T01 MTR_2g022020 40.517 116 69 0 190 305 1 116 8.18e-24 94.4
MsG0880043802.01.T01 MTR_1g076810 36.158 177 86 5 1 151 55 230 8.41e-24 102
MsG0880043802.01.T01 MTR_7g101200 31.416 226 116 10 62 273 36 236 2.00e-23 96.7
MsG0880043802.01.T01 MTR_5g036250 48.276 87 45 0 207 293 8 94 3.42e-23 94.7
MsG0880043802.01.T01 MTR_5g091150 39.355 155 66 5 65 219 60 186 3.97e-22 92.0
MsG0880043802.01.T01 MTR_2g104260 34.348 230 101 11 5 187 51 277 4.53e-22 94.0
MsG0880043802.01.T01 MTR_1g047080 29.042 334 155 16 1 306 1 280 7.66e-19 85.1
MsG0880043802.01.T01 MTR_4g094400 40.476 126 48 4 156 265 7 121 6.25e-18 79.0
MsG0880043802.01.T01 MTR_3g097190 53.704 54 25 0 231 284 28 81 1.11e-13 68.6
MsG0880043802.01.T01 MTR_6g070880 29.843 191 91 8 3 151 71 260 1.14e-13 70.1
MsG0880043802.01.T01 MTR_6g071420 29.843 191 91 8 3 151 71 260 1.14e-13 70.1
MsG0880043802.01.T01 MTR_3g047540 42.353 85 43 4 127 210 4 83 7.32e-13 63.9
MsG0880043802.01.T01 MTR_5g037190 26.818 220 102 8 1 182 53 251 8.84e-13 67.4
MsG0880043802.01.T01 MTR_8g041440 65.385 52 3 1 1 52 52 88 1.24e-12 63.9
MsG0880043802.01.T01 MTR_4g023130 24.046 262 172 10 52 305 64 306 1.29e-12 67.8
MsG0880043802.01.T01 MTR_4g070550 24.528 265 168 12 64 304 124 380 4.83e-12 66.2
MsG0880043802.01.T01 MTR_4g023080 24.324 259 169 10 55 305 108 347 5.09e-12 66.2
MsG0880043802.01.T01 MTR_4g061340 26.415 212 127 5 67 266 141 335 6.19e-12 65.9
MsG0880043802.01.T01 MTR_8g433040 22.047 254 184 6 55 305 115 357 7.07e-12 65.9
MsG0880043802.01.T01 MTR_8g032380 22.047 254 184 6 55 305 115 357 7.07e-12 65.9
MsG0880043802.01.T01 MTR_4g116330 23.950 238 159 7 55 284 125 348 7.70e-12 65.9
MsG0880043802.01.T01 MTR_5g049520 64.286 42 15 0 251 292 22 63 1.63e-11 62.0
MsG0880043802.01.T01 MTR_3g083010 24.537 216 142 8 55 256 122 330 1.71e-11 64.7
MsG0880043802.01.T01 MTR_4g023070 23.443 273 176 11 55 305 102 363 2.25e-11 64.3
MsG0880043802.01.T01 MTR_1g050305 44.578 83 41 2 212 289 21 103 2.56e-11 61.2
MsG0880043802.01.T01 MTR_5g097730 63.889 36 13 0 236 271 18 53 3.48e-11 58.2
MsG0880043802.01.T01 MTR_1g009110 24.427 262 165 11 30 273 94 340 3.71e-11 63.5
MsG0880043802.01.T01 MTR_6g060650 25.461 271 168 13 52 305 113 366 6.99e-11 62.8
MsG0880043802.01.T01 MTR_1g097560 24.710 259 170 11 52 303 132 372 9.12e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043802.01.T01 AT3G06240 24.891 229 148 10 61 280 178 391 4.00e-13 69.7
MsG0880043802.01.T01 AT4G12560 22.388 268 168 11 65 303 108 364 1.07e-12 68.6
MsG0880043802.01.T01 AT4G12560 22.388 268 168 11 65 303 108 364 1.07e-12 68.6
MsG0880043802.01.T01 AT4G12560 22.388 268 168 11 65 303 108 364 1.07e-12 68.6

Find 54 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATATCACATTTCAATTT+TGG 0.076707 8:-30935814 MsG0880043802.01.T01:CDS
CTCACTCAAGTGTTTCATTC+TGG 0.152822 8:-30936121 MsG0880043802.01.T01:CDS
TCTGCATTCTTACTACTATA+TGG 0.220601 8:-30936326 MsG0880043802.01.T01:CDS
CGGCGTTAAAGATGACTTTA+TGG 0.256773 8:-30936180 MsG0880043802.01.T01:CDS
TTCTTACTACTATATGGAAT+TGG 0.257825 8:-30936320 MsG0880043802.01.T01:CDS
CTTGACGTCTTGTCAGATTT+AGG 0.270260 8:+30936533 None:intergenic
TTGGAAGAATGCTTTCTATA+TGG 0.275419 8:+30935595 None:intergenic
CCTTGCCTTGAGAATCCTAT+TGG 0.308206 8:-30935738 MsG0880043802.01.T01:CDS
TAACTATATCTTCATCCAAT+TGG 0.352415 8:+30935576 None:intergenic
CCATTCTTCCCCACTCCAAT+AGG 0.359381 8:+30935723 None:intergenic
AGAATAGAGTTAAATTAGAT+TGG 0.362567 8:-30936474 MsG0880043802.01.T01:CDS
GGTTTCAACAATAAAGAGTT+TGG 0.366127 8:+30935764 None:intergenic
TCACTCAAGTGTTTCATTCT+GGG 0.369909 8:-30936120 MsG0880043802.01.T01:CDS
ATAGAAAGCATTCTTCCAAT+TGG 0.400066 8:-30935591 MsG0880043802.01.T01:CDS
TCTTACTACTATATGGAATT+GGG 0.415758 8:-30936319 MsG0880043802.01.T01:CDS
CATGTGCACTTCTTCAGTTT+CGG 0.424484 8:+30936031 None:intergenic
CCTATTGGAGTGGGGAAGAA+TGG 0.443662 8:-30935723 MsG0880043802.01.T01:CDS
AATTATCTTGTTTGTTATGA+CGG 0.444388 8:-30936200 MsG0880043802.01.T01:CDS
TTTGGGTGAACCCGGTGTAA+TGG 0.446677 8:-30935796 MsG0880043802.01.T01:CDS
AAGATGACTTTATGGTGATT+CGG 0.449172 8:-30936172 MsG0880043802.01.T01:CDS
AGAAACTTAATGATATTGAA+TGG 0.450606 8:-30935873 MsG0880043802.01.T01:CDS
TCTTCAGTTTCGGTATAAGT+TGG 0.453213 8:+30936041 None:intergenic
GTCTTGAGTTCATTGGTAGT+TGG 0.461601 8:+30936278 None:intergenic
AGGAATTGTCTTGAGTTCAT+TGG 0.465114 8:+30936271 None:intergenic
GTTGGTATAACTTAAGTAGC+AGG 0.498011 8:-30935665 MsG0880043802.01.T01:CDS
ACATGGATGGGATGTCTCAT+TGG 0.500107 8:-30936013 MsG0880043802.01.T01:CDS
GAAACTTAATGATATTGAAT+GGG 0.506187 8:-30935872 MsG0880043802.01.T01:CDS
TCAGTTTCGGTATAAGTTGG+AGG 0.507885 8:+30936044 None:intergenic
CTTGAGTGAGGATCATCAAA+TGG 0.523089 8:+30936134 None:intergenic
AACCCGGTGTAATGGAATCA+TGG 0.530502 8:-30935788 MsG0880043802.01.T01:CDS
CTTGAGAATCCTATTGGAGT+GGG 0.531650 8:-30935732 MsG0880043802.01.T01:CDS
ACTGAAGAAGTGCACATGGA+TGG 0.532324 8:-30936026 MsG0880043802.01.T01:CDS
CCAATAGGATTCTCAAGGCA+AGG 0.541785 8:+30935738 None:intergenic
GGTCCATGATTCCATTACAC+CGG 0.541825 8:+30935785 None:intergenic
ATACCCTCTGACATTGATGA+TGG 0.548910 8:-30935918 MsG0880043802.01.T01:CDS
GGATGGGATGTCTCATTGGT+TGG 0.554386 8:-30936009 MsG0880043802.01.T01:CDS
ACATATCATGATAATAAGAA+TGG 0.556921 8:+30936563 None:intergenic
GCTTTCTATATGGAATAGTA+CGG 0.560125 8:+30935605 None:intergenic
GTCCATGATTCCATTACACC+GGG 0.574882 8:+30935786 None:intergenic
CCTTGAGAATCCTATTGGAG+TGG 0.581815 8:-30935733 MsG0880043802.01.T01:CDS
AGGATTCTCAAGGCAAGGGA+AGG 0.585026 8:+30935743 None:intergenic
CTGAAGAAGTGCACATGGAT+GGG 0.586443 8:-30936025 MsG0880043802.01.T01:CDS
CCGCAATAACTTCTTATCAA+AGG 0.593540 8:-30936587 MsG0880043802.01.T01:CDS
CAATAGGATTCTCAAGGCAA+GGG 0.601919 8:+30935739 None:intergenic
CGAAACTGAAGAAGTGCACA+TGG 0.601972 8:-30936030 MsG0880043802.01.T01:CDS
CCACTCCAATAGGATTCTCA+AGG 0.615978 8:+30935733 None:intergenic
AGAACCATCATCAATGTCAG+AGG 0.618203 8:+30935914 None:intergenic
GAACCATCATCAATGTCAGA+GGG 0.639138 8:+30935915 None:intergenic
TGATTAACATACGACTGATG+AGG 0.663206 8:+30936227 None:intergenic
ATTGGTAGTTGGATTCCACA+AGG 0.663464 8:+30936289 None:intergenic
GAATTGGGAAACTTACCTTG+TGG 0.674577 8:-30936304 MsG0880043802.01.T01:CDS
CCTTTGATAAGAAGTTATTG+CGG 0.679677 8:+30936587 None:intergenic
CAGAATGAAACACTTGAGTG+AGG 0.680568 8:+30936122 None:intergenic
TTGAGAATCCTATTGGAGTG+GGG 0.684791 8:-30935731 MsG0880043802.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTATTTAGAAAAAAAGA+CGG - Chr8:30936489-30936508 MsG0880043802.01.T01:CDS 15.0%
!!! ATGTTTTGTATTTACTTTTT+GGG - Chr8:30935678-30935697 MsG0880043802.01.T01:CDS 15.0%
!!! GTTCTAATAATATTTTAGTA+TGG - Chr8:30936289-30936308 MsG0880043802.01.T01:CDS 15.0%
!! ACATATCATGATAATAAGAA+TGG + Chr8:30935625-30935644 None:intergenic 20.0%
!! AGAAACTTAATGATATTGAA+TGG - Chr8:30936312-30936331 MsG0880043802.01.T01:CDS 20.0%
!! AGAATAGAGTTAAATTAGAT+TGG - Chr8:30935711-30935730 MsG0880043802.01.T01:CDS 20.0%
!! GAAACTTAATGATATTGAAT+GGG - Chr8:30936313-30936332 MsG0880043802.01.T01:CDS 20.0%
!!! AATTATCTTGTTTGTTATGA+CGG - Chr8:30935985-30936004 MsG0880043802.01.T01:CDS 20.0%
!!! ACATATCACATTTCAATTTT+GGG - Chr8:30936372-30936391 MsG0880043802.01.T01:intron 20.0%
!!! CATGTTTTGTATTTACTTTT+TGG - Chr8:30935677-30935696 MsG0880043802.01.T01:CDS 20.0%
!!! TACATATCACATTTCAATTT+TGG - Chr8:30936371-30936390 MsG0880043802.01.T01:intron 20.0%
!!! TGTTTTGTATTTACTTTTTG+GGG - Chr8:30935679-30935698 MsG0880043802.01.T01:CDS 20.0%
! TCTTACTACTATATGGAATT+GGG - Chr8:30935866-30935885 MsG0880043802.01.T01:CDS 25.0%
! TTCTTACTACTATATGGAAT+TGG - Chr8:30935865-30935884 MsG0880043802.01.T01:CDS 25.0%
AAAAAGACGGTGAAATATGT+TGG - Chr8:30936502-30936521 MsG0880043802.01.T01:CDS 30.0%
AAGATGACTTTATGGTGATT+CGG - Chr8:30936013-30936032 MsG0880043802.01.T01:CDS 30.0%
ACCCAAAATTTCAAAACAAG+AGG + Chr8:30935813-30935832 None:intergenic 30.0%
CCTTTGATAAGAAGTTATTG+CGG + Chr8:30935601-30935620 None:intergenic 30.0%
GCTTTCTATATGGAATAGTA+CGG + Chr8:30936583-30936602 None:intergenic 30.0%
GGTTTCAACAATAAAGAGTT+TGG + Chr8:30936424-30936443 None:intergenic 30.0%
TCTGCATTCTTACTACTATA+TGG - Chr8:30935859-30935878 MsG0880043802.01.T01:CDS 30.0%
TTGGAAGAATGCTTTCTATA+TGG + Chr8:30936593-30936612 None:intergenic 30.0%
! TAAATTAGATTGGCCAAAAG+TGG - Chr8:30935721-30935740 MsG0880043802.01.T01:CDS 30.0%
!! ATAGAAAGCATTCTTCCAAT+TGG - Chr8:30936594-30936613 MsG0880043802.01.T01:CDS 30.0%
!!! GCCTCTTGTTTTGAAATTTT+GGG - Chr8:30935809-30935828 MsG0880043802.01.T01:CDS 30.0%
!!! TGCCTCTTGTTTTGAAATTT+TGG - Chr8:30935808-30935827 MsG0880043802.01.T01:CDS 30.0%
!!! TGTATTTACTTTTTGGGGAA+AGG - Chr8:30935684-30935703 MsG0880043802.01.T01:CDS 30.0%
ACACAAAAATCAGGAATGGA+AGG + Chr8:30935937-30935956 None:intergenic 35.0%
AGGAATTGTCTTGAGTTCAT+TGG + Chr8:30935917-30935936 None:intergenic 35.0%
AGGTACACAAAAATCAGGAA+TGG + Chr8:30935941-30935960 None:intergenic 35.0%
CCGCAATAACTTCTTATCAA+AGG - Chr8:30935598-30935617 MsG0880043802.01.T01:CDS 35.0%
CTCTTGGAGAAAAATTGACA+TGG - Chr8:30936110-30936129 MsG0880043802.01.T01:CDS 35.0%
GTTGGTATAACTTAAGTAGC+AGG - Chr8:30936520-30936539 MsG0880043802.01.T01:CDS 35.0%
TCACTCAAGTGTTTCATTCT+GGG - Chr8:30936065-30936084 MsG0880043802.01.T01:CDS 35.0%
TCATCATTGTCCTCTTGAAT+AGG + Chr8:30935764-30935783 None:intergenic 35.0%
TCTTCAGTTTCGGTATAAGT+TGG + Chr8:30936147-30936166 None:intergenic 35.0%
TGATGAGGTACACAAAAATC+AGG + Chr8:30935946-30935965 None:intergenic 35.0%
TGATTAACATACGACTGATG+AGG + Chr8:30935961-30935980 None:intergenic 35.0%
!! ATTTCAATTTTGGGTGAACC+CGG - Chr8:30936381-30936400 MsG0880043802.01.T01:intron 35.0%
AGAACCATCATCAATGTCAG+AGG + Chr8:30936274-30936293 None:intergenic 40.0%
ATACCCTCTGACATTGATGA+TGG - Chr8:30936267-30936286 MsG0880043802.01.T01:CDS 40.0%
ATAGCCTACGCAAAAACTCT+TGG - Chr8:30936094-30936113 MsG0880043802.01.T01:CDS 40.0%
CAATAGGATTCTCAAGGCAA+GGG + Chr8:30936449-30936468 None:intergenic 40.0%
CAGAATGAAACACTTGAGTG+AGG + Chr8:30936066-30936085 None:intergenic 40.0%
CATGTGCACTTCTTCAGTTT+CGG + Chr8:30936157-30936176 None:intergenic 40.0%
CGGCGTTAAAGATGACTTTA+TGG - Chr8:30936005-30936024 MsG0880043802.01.T01:CDS 40.0%
CTCACTCAAGTGTTTCATTC+TGG - Chr8:30936064-30936083 MsG0880043802.01.T01:CDS 40.0%
CTTGACGTCTTGTCAGATTT+AGG + Chr8:30935655-30935674 None:intergenic 40.0%
CTTGAGAATCCTATTGGAGT+GGG - Chr8:30936453-30936472 MsG0880043802.01.T01:intron 40.0%
CTTGAGTGAGGATCATCAAA+TGG + Chr8:30936054-30936073 None:intergenic 40.0%
GAACCATCATCAATGTCAGA+GGG + Chr8:30936273-30936292 None:intergenic 40.0%
GAATTGGGAAACTTACCTTG+TGG - Chr8:30935881-30935900 MsG0880043802.01.T01:CDS 40.0%
TGACACACAACCTATTCAAG+AGG - Chr8:30935751-30935770 MsG0880043802.01.T01:CDS 40.0%
TTGAGAATCCTATTGGAGTG+GGG - Chr8:30936454-30936473 MsG0880043802.01.T01:intron 40.0%
! ATTGGTAGTTGGATTCCACA+AGG + Chr8:30935899-30935918 None:intergenic 40.0%
! GTCTTGAGTTCATTGGTAGT+TGG + Chr8:30935910-30935929 None:intergenic 40.0%
! TAGATTGGCCAAAAGTGGAT+GGG - Chr8:30935726-30935745 MsG0880043802.01.T01:CDS 40.0%
! TCAGTTTCGGTATAAGTTGG+AGG + Chr8:30936144-30936163 None:intergenic 40.0%
! TTAGATTGGCCAAAAGTGGA+TGG - Chr8:30935725-30935744 MsG0880043802.01.T01:CDS 40.0%
! TTCTCCAAGAGTTTTTGCGT+AGG + Chr8:30936101-30936120 None:intergenic 40.0%
ACATGGATGGGATGTCTCAT+TGG - Chr8:30936172-30936191 MsG0880043802.01.T01:CDS 45.0%
CCAATAGGATTCTCAAGGCA+AGG + Chr8:30936450-30936469 None:intergenic 45.0%
CCACTCCAATAGGATTCTCA+AGG + Chr8:30936455-30936474 None:intergenic 45.0%
CCTCATCCAAGTCATATGGT+GGG + Chr8:30935787-30935806 None:intergenic 45.0%
CCTTGAGAATCCTATTGGAG+TGG - Chr8:30936452-30936471 MsG0880043802.01.T01:intron 45.0%
CCTTGCCTTGAGAATCCTAT+TGG - Chr8:30936447-30936466 MsG0880043802.01.T01:intron 45.0%
GCATCCTCATCCAAGTCATA+TGG + Chr8:30935791-30935810 None:intergenic 45.0%
GGTCCATGATTCCATTACAC+CGG + Chr8:30936403-30936422 None:intergenic 45.0%
GTCCATGATTCCATTACACC+GGG + Chr8:30936402-30936421 None:intergenic 45.0%
TCCTCATCCAAGTCATATGG+TGG + Chr8:30935788-30935807 None:intergenic 45.0%
TGATGACCCACCATATGACT+TGG - Chr8:30935778-30935797 MsG0880043802.01.T01:CDS 45.0%
TTGGGTTCAGCGAGTGTTAA+TGG - Chr8:30935827-30935846 MsG0880043802.01.T01:CDS 45.0%
! AACCCGGTGTAATGGAATCA+TGG - Chr8:30936397-30936416 MsG0880043802.01.T01:intron 45.0%
! ACTGAAGAAGTGCACATGGA+TGG - Chr8:30936159-30936178 MsG0880043802.01.T01:CDS 45.0%
! AGATTGGCCAAAAGTGGATG+GGG - Chr8:30935727-30935746 MsG0880043802.01.T01:CDS 45.0%
! CGAAACTGAAGAAGTGCACA+TGG - Chr8:30936155-30936174 MsG0880043802.01.T01:CDS 45.0%
! CTGAAGAAGTGCACATGGAT+GGG - Chr8:30936160-30936179 MsG0880043802.01.T01:CDS 45.0%
AGGATTCTCAAGGCAAGGGA+AGG + Chr8:30936445-30936464 None:intergenic 50.0%
CCATTCTTCCCCACTCCAAT+AGG + Chr8:30936465-30936484 None:intergenic 50.0%
CCTATTGGAGTGGGGAAGAA+TGG - Chr8:30936462-30936481 MsG0880043802.01.T01:intron 50.0%
GGATGGGATGTCTCATTGGT+TGG - Chr8:30936176-30936195 MsG0880043802.01.T01:CDS 50.0%
GTTCAGCGAGTGTTAATGGC+AGG - Chr8:30935831-30935850 MsG0880043802.01.T01:CDS 50.0%
TTTGGGTGAACCCGGTGTAA+TGG - Chr8:30936389-30936408 MsG0880043802.01.T01:intron 50.0%
! CCCACCATATGACTTGGATG+AGG - Chr8:30935784-30935803 MsG0880043802.01.T01:CDS 50.0%
! GTGTCATCCCCATCCACTTT+TGG + Chr8:30935737-30935756 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 30935587 30936620 30935587 ID=MsG0880043802.01;Name=MsG0880043802.01
Chr8 mRNA 30935587 30936620 30935587 ID=MsG0880043802.01.T01;Parent=MsG0880043802.01;Name=MsG0880043802.01.T01;_AED=0.33;_eAED=0.33;_QI=0|0|0|1|1|1|2|0|306
Chr8 exon 30936468 30936620 30936468 ID=MsG0880043802.01.T01:exon:9279;Parent=MsG0880043802.01.T01
Chr8 exon 30935587 30936354 30935587 ID=MsG0880043802.01.T01:exon:9278;Parent=MsG0880043802.01.T01
Chr8 CDS 30936468 30936620 30936468 ID=MsG0880043802.01.T01:cds;Parent=MsG0880043802.01.T01
Chr8 CDS 30935587 30936354 30935587 ID=MsG0880043802.01.T01:cds;Parent=MsG0880043802.01.T01
Gene Sequence

>MsG0880043802.01.T01

ATGACTATGCACCGCAATAACTTCTTATCAAAGGACCATTCTTATTATCATGATATGTCTCTTCTCCTAAATCTGACAAGACGTCAAGAACATGTTTTGTATTTACTTTTTGGGGAAAGGTTTGAGAATAGAGTTAAATTAGATTGGCCAAAAGCTTTGTCTGCATTCTTACTACTATATGGAATTGGGAAACTTACCTTGTGGAATCCAACTACCAATGAACTCAAGACAATTCCTTCCATTCCTGATTTTTGTGTACCTCATCAGTCGTATGTTAATCACATTAATTATCTTGTTTGTTATGACGGCGTTAAAGATGACTTTATGGTGATTCGGTTCACAACTTGCAGACCATTTGATGATCCTCACTCAAGTGTTTCATTCTGGGTGATATATAGCCTACGCAAAAACTCTTGGAGAAAAATTGACATGGATCATATGCCTCCAACTTATACCGAAACTGAAGAAGTGCACATGGATGGGATGTCTCATTGGTTGGATCAAAGAAGTACATATATATTTTTGCTGTCATTTGACTTCAGCAATGAATCTTTCATTACAACAGCTATACCCTCTGACATTGATGATGGTTCTAATAATATTTTAGTATGGAGAAACTTAATGATATTGAATGGGTCTATTGCTTTCATCTCAAATTATGAAGAGACATCTACATATCACATTTCAATTTTGGGTGAACCCGGTGTAATGGAATCATGGACCAAACTCTTTATTGTTGAAACCTTCCCTTGCCTTGAGAATCCTATTGGAGTGGGGAAGAATGGAAATATACTATTTAGAAAAAAAGACGGTGAAATATGTTGGTATAACTTAAGTAGCAGGATGATTGACGAGATTATTGTTAGAGCAGAGAGTTGTTCCGTACTATTCCATATAGAAAGCATTCTTCCAATTGGATGA
Protein sequence

>MsG0880043802.01.T01

MTMHRNNFLSKDHSYYHDMSLLLNLTRRQEHVLYLLFGERFENRVKLDWPKALSAFLLLYGIGKLTLWNPTTNELKTIPSIPDFCVPHQSYVNHINYLVCYDGVKDDFMVIRFTTCRPFDDPHSSVSFWVIYSLRKNSWRKIDMDHMPPTYTETEEVHMDGMSHWLDQRSTYIFLLSFDFSNESFITTAIPSDIDDGSNNILVWRNLMILNGSIAFISNYEETSTYHISILGEPGVMESWTKLFIVETFPCLENPIGVGKNGNILFRKKDGEICWYNLSSRMIDEIIVRAESCSVLFHIESILPIG*