Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043918.01.T01 | XP_039685525.1 | 94.177 | 498 | 28 | 1 | 1 | 497 | 1 | 498 | 0 | 969 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280007902.01 | MsG0880043918.01 | 0.856120 | 3.986727e-62 | 9.415097e-59 |
| MsG0280010131.01 | MsG0880043918.01 | 0.841629 | 4.230837e-58 | 6.253918e-55 |
| MsG0380015988.01 | MsG0880043918.01 | 0.855390 | 6.513405e-62 | 1.501543e-58 |
| MsG0480018096.01 | MsG0880043918.01 | 0.809446 | 1.885116e-50 | 1.107022e-47 |
| MsG0480020522.01 | MsG0880043918.01 | 0.837886 | 3.988403e-57 | 5.252920e-54 |
| MsG0880042650.01 | MsG0880043918.01 | 0.821413 | 4.092003e-53 | 3.326723e-50 |
| MsG0880042921.01 | MsG0880043918.01 | 0.804747 | 1.862034e-49 | 9.678316e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043918.01.T01 | MTR_1g040310 | 89.200 | 500 | 49 | 4 | 1 | 498 | 1 | 497 | 0.0 | 908 |
| MsG0880043918.01.T01 | MTR_8g044140 | 93.696 | 460 | 28 | 1 | 39 | 497 | 1 | 460 | 0.0 | 896 |
| MsG0880043918.01.T01 | MTR_5g016660 | 65.496 | 484 | 162 | 3 | 14 | 493 | 9 | 491 | 0.0 | 667 |
| MsG0880043918.01.T01 | MTR_5g039900 | 62.910 | 488 | 173 | 5 | 14 | 496 | 12 | 496 | 0.0 | 620 |
| MsG0880043918.01.T01 | MTR_5g040030 | 55.375 | 493 | 217 | 3 | 10 | 499 | 9 | 501 | 0.0 | 568 |
| MsG0880043918.01.T01 | MTR_6g035295 | 57.645 | 484 | 199 | 5 | 10 | 489 | 7 | 488 | 0.0 | 558 |
| MsG0880043918.01.T01 | MTR_7g070910 | 52.893 | 484 | 223 | 4 | 14 | 493 | 10 | 492 | 0.0 | 530 |
| MsG0880043918.01.T01 | MTR_7g070800 | 52.273 | 484 | 226 | 5 | 11 | 492 | 2 | 482 | 0.0 | 528 |
| MsG0880043918.01.T01 | MTR_7g070610 | 52.000 | 500 | 227 | 6 | 1 | 493 | 1 | 494 | 0.0 | 526 |
| MsG0880043918.01.T01 | MTR_7g070740 | 52.772 | 487 | 226 | 4 | 9 | 492 | 5 | 490 | 0.0 | 526 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043918.01.T01 | AT2G15480 | 39.308 | 491 | 272 | 8 | 17 | 489 | 12 | 494 | 1.08e-118 | 358 |
| MsG0880043918.01.T01 | AT2G15490 | 40.249 | 482 | 270 | 8 | 17 | 489 | 9 | 481 | 5.49e-118 | 355 |
| MsG0880043918.01.T01 | AT2G15480 | 39.627 | 482 | 273 | 8 | 17 | 489 | 12 | 484 | 2.65e-117 | 354 |
| MsG0880043918.01.T01 | AT2G15490 | 40.000 | 485 | 270 | 9 | 17 | 489 | 9 | 484 | 3.11e-116 | 351 |
| MsG0880043918.01.T01 | AT4G34131 | 40.947 | 486 | 262 | 12 | 14 | 487 | 9 | 481 | 2.94e-114 | 346 |
| MsG0880043918.01.T01 | AT4G34138 | 40.726 | 496 | 265 | 13 | 10 | 492 | 6 | 485 | 7.73e-113 | 343 |
| MsG0880043918.01.T01 | AT2G36780 | 39.709 | 481 | 276 | 8 | 14 | 485 | 13 | 488 | 1.21e-112 | 342 |
| MsG0880043918.01.T01 | AT2G36770 | 38.125 | 480 | 285 | 8 | 14 | 485 | 13 | 488 | 3.21e-108 | 331 |
| MsG0880043918.01.T01 | AT4G34135 | 40.166 | 483 | 271 | 9 | 14 | 487 | 10 | 483 | 5.92e-108 | 330 |
| MsG0880043918.01.T01 | AT2G36760 | 38.809 | 487 | 272 | 10 | 14 | 485 | 13 | 488 | 3.29e-107 | 328 |
Find 119 sgRNAs with CRISPR-Local
Find 137 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCACCATCACCGTCTT+CGG | 0.441273 | 8:+32560912 | None:intergenic |
| AAAATCGAAGATGCCGAAGA+CGG | 0.584835 | 8:-32560925 | MsG0880043918.01.T01:CDS |
| AAAGATGATTCAGATAAAGC+TGG | 0.548782 | 8:-32561126 | MsG0880043918.01.T01:CDS |
| AAAGGCAATAGGTTTATTGA+TGG | 0.446677 | 8:-32560584 | MsG0880043918.01.T01:CDS |
| AAAGGTTAATAACAAATTGG+AGG | 0.550002 | 8:-32560422 | MsG0880043918.01.T01:CDS |
| AACAAAGGCTATTTGATATG+GGG | 0.584351 | 8:-32560856 | MsG0880043918.01.T01:CDS |
| AACAATGGCCAAGTAGCCAA+TGG | 0.614554 | 8:+32560733 | None:intergenic |
| AAGAAAGCCAAAGAACTAGG+TGG | 0.745027 | 8:-32560532 | MsG0880043918.01.T01:CDS |
| AAGAAAGCTATAGAGGTTGG+CGG | 0.616772 | 8:-32560502 | MsG0880043918.01.T01:CDS |
| AAGGCAATAGGTTTATTGAT+GGG | 0.527651 | 8:-32560583 | MsG0880043918.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAACTGTTCAGCAAACAA+TGG | + | Chr8:32561590-32561609 | None:intergenic | 30.0% | |
| AAAGATGATTCAGATAAAGC+TGG | - | Chr8:32561179-32561198 | MsG0880043918.01.T01:CDS | 30.0% | |
| AAAGGCAATAGGTTTATTGA+TGG | - | Chr8:32561721-32561740 | MsG0880043918.01.T01:CDS | 30.0% | |
| AACAAAGGCTATTTGATATG+GGG | - | Chr8:32561449-32561468 | MsG0880043918.01.T01:CDS | 30.0% | |
| AAGGCAATAGGTTTATTGAT+GGG | - | Chr8:32561722-32561741 | MsG0880043918.01.T01:CDS | 30.0% | |
| CAACAAAGGCTATTTGATAT+GGG | - | Chr8:32561448-32561467 | MsG0880043918.01.T01:CDS | 30.0% | |
| CATAATTTCCAAAATCTCTC+AGG | - | Chr8:32560701-32560720 | MsG0880043918.01.T01:CDS | 30.0% | |
| GATTATTACAAGATAGCCAT+GGG | - | Chr8:32561113-32561132 | MsG0880043918.01.T01:CDS | 30.0% | |
| GATTCAGAGAGAAAAAGTTA+TGG | - | Chr8:32561047-32561066 | MsG0880043918.01.T01:CDS | 30.0% | |
| GTAAAGAAGAAGATGAAGAA+GGG | - | Chr8:32561213-32561232 | MsG0880043918.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 32560411 | 32561916 | 32560411 | ID=MsG0880043918.01;Name=MsG0880043918.01 |
| Chr8 | mRNA | 32560411 | 32561916 | 32560411 | ID=MsG0880043918.01.T01;Parent=MsG0880043918.01;Name=MsG0880043918.01.T01;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|501 |
| Chr8 | exon | 32560411 | 32561916 | 32560411 | ID=MsG0880043918.01.T01:exon:8033;Parent=MsG0880043918.01.T01 |
| Chr8 | CDS | 32560411 | 32561916 | 32560411 | ID=MsG0880043918.01.T01:cds;Parent=MsG0880043918.01.T01 |
| Gene Sequence |
>MsG0880043918.01.T01 ATGGAATCCTTTGGTGTCAAAGTTGAAGAAGAAACAATGTTAAAAGCTGTTTTTCTCCCCTTTATATCAAAAAGTCACCTCATTTTCGTAGTAGACATAGCAAGGCTTTTTGCTATGCATGGTGTAGATGTAACCATAATCACCACACCAGCAAATGCAGCCATTTTTCAAACCTCCATCGACCGCGATTCAAGTCGCGGTCGGTCCATTAGAACTCACGTTGTTCAGTTTCCACAAGTACCTGGTTTGCCACAAGGAATGGAAAGTTTCAATGCTGACACTCCTAAAGACATAATTTCCAAAATCTCTCAGGGACTTTCCATTCTCCAAGAGCAATTCACACAACTATTTCGTGACATGAAACCTGATTTCATAGTCACTGACATGTTTTATCCTTGGAGTGTTGATGTTGCTGATGAATTGGGAATTCCAAGGTTGGTTTGTATTGGTGGAAGTTACTTTGCTCATTCTGCAATGAACTCTGTTGAACAATTTGAACCTCATGCGAAAGTTAAATCGAATAGCGAGAGTTTTCTTCTTCCTGGGCTGCCTCACAACGTGGAAATGACGCGTTTGCAACTTCCGGATTGGCTTAGAGCACCGAATGGTTATACTTATTTGATGAAGATGATTAAAGATTCAGAGAGAAAAAGTTATGGATCATTGTTTGATACCTATTATGATATTGAAGGTACTTATGAGGATTATTACAAGATAGCCATGGGAAGTAAGAGTTGGAGTGTGGGACCAGTTTCTTTGTGGATGAACAAAGATGATTCAGATAAAGCTGGTAGAGGACATGGTAAAGAAGAAGATGAAGAAGGGGTGATTAAGTGGCTTGATTCAAAGAAAGATGATTCTGTTCTCTATGTTAGTTTTGGGAGCATGAACAAGTTCCCTATTCCTCAACTTGTTGAAATAGCTCATGCACTTGAAGATTCTGGCCATGATTTCATTTGGGTTGTTAGAAAAATCGAAGATGCCGAAGACGGTGATGGTGGTTTTTTGATGGAGTTTGAGAAGAGAATGAAGGAAAGCAACAAAGGCTATTTGATATGGGGTTGGGCACCACAACTTTTGATACTAGAGCATGCTGCAGTTGGAGCAGTGGTGACTCATTGTGGGTGGAATACTATTATGGAAAGTGTAAATGCTGGTTTGCCATTGGCTACTTGGCCATTGTTTGCTGAACAGTTTTTCAATGAGAGATTGTTGGTTGATGTGTTGAGGATTGGTGTGGCAGTTGGGGCAAAAGAATGGAGAAATTGGAATGAGTTTGGGGATGATGTGGTTAAAAAGGAGGATATAGGAAAGGCAATAGGTTTATTGATGGGAGGTGGAGAAGAGTGTTTAGAAATGAGGAAGAAAGCCAAAGAACTAGGTGGTGCTGCAAAGAAAGCTATAGAGGTTGGCGGTTCTTCTTACACTAAGTTGAAAGAACTAATCGAGGAGCTCAAGTCATTCAAACTTGGAAAGGTTAATAACAAATTGGAGGGACTATGTTGA |
| Protein sequence |
>MsG0880043918.01.T01 MESFGVKVEEETMLKAVFLPFISKSHLIFVVDIARLFAMHGVDVTIITTPANAAIFQTSIDRDSSRGRSIRTHVVQFPQVPGLPQGMESFNADTPKDIISKISQGLSILQEQFTQLFRDMKPDFIVTDMFYPWSVDVADELGIPRLVCIGGSYFAHSAMNSVEQFEPHAKVKSNSESFLLPGLPHNVEMTRLQLPDWLRAPNGYTYLMKMIKDSERKSYGSLFDTYYDIEGTYEDYYKIAMGSKSWSVGPVSLWMNKDDSDKAGRGHGKEEDEEGVIKWLDSKKDDSVLYVSFGSMNKFPIPQLVEIAHALEDSGHDFIWVVRKIEDAEDGDGGFLMEFEKRMKESNKGYLIWGWAPQLLILEHAAVGAVVTHCGWNTIMESVNAGLPLATWPLFAEQFFNERLLVDVLRIGVAVGAKEWRNWNEFGDDVVKKEDIGKAIGLLMGGGEECLEMRKKAKELGGAAKKAIEVGGSSYTKLKELIEELKSFKLGKVNNKLEGLC* |
