Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020522.01.T01 | XP_024636864.1 | 93.333 | 165 | 10 | 1 | 1 | 165 | 1 | 164 | 2.07E-106 | 315 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020522.01.T01 | A0A072UVF9 | 93.333 | 165 | 10 | 1 | 1 | 165 | 1 | 164 | 1.24e-106 | 314 |
| MsG0480020522.01.T01 | I3SLC9 | 93.333 | 165 | 10 | 1 | 1 | 165 | 1 | 164 | 1.13e-106 | 313 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048256.01 | MsG0480020522.01 | -0.815479 | 9.050146e-52 | 6.247157e-49 |
| MsG0080048929.01 | MsG0480020522.01 | 0.827790 | 1.287914e-54 | 1.256060e-51 |
| MsG0080049053.01 | MsG0480020522.01 | 0.817255 | 3.625503e-52 | 2.626991e-49 |
| MsG0180000193.01 | MsG0480020522.01 | 0.811151 | 8.081534e-51 | 4.964748e-48 |
| MsG0180000305.01 | MsG0480020522.01 | 0.814822 | 1.266474e-51 | 8.586412e-49 |
| MsG0180000306.01 | MsG0480020522.01 | 0.855052 | 8.167894e-62 | 1.861649e-58 |
| MsG0180000325.01 | MsG0480020522.01 | 0.831061 | 2.066861e-55 | 2.217963e-52 |
| MsG0180001113.01 | MsG0480020522.01 | 0.806595 | 7.620784e-50 | 4.152864e-47 |
| MsG0180003296.01 | MsG0480020522.01 | 0.821469 | 3.970579e-53 | 3.232996e-50 |
| MsG0180003933.01 | MsG0480020522.01 | 0.820255 | 7.553458e-53 | 5.946509e-50 |
| MsG0180003980.01 | MsG0480020522.01 | 0.803717 | 3.050512e-49 | 1.543948e-46 |
| MsG0180004177.01 | MsG0480020522.01 | 0.802421 | 5.654362e-49 | 2.768746e-46 |
| MsG0180004292.01 | MsG0480020522.01 | 0.801875 | 7.325263e-49 | 3.537369e-46 |
| MsG0180004302.01 | MsG0480020522.01 | 0.817049 | 4.034307e-52 | 2.906936e-49 |
| MsG0180004386.01 | MsG0480020522.01 | 0.817384 | 3.391408e-52 | 2.466270e-49 |
| MsG0180004852.01 | MsG0480020522.01 | 0.867601 | 1.213657e-65 | 4.261742e-62 |
| MsG0180005431.01 | MsG0480020522.01 | 0.804535 | 2.062071e-49 | 1.065822e-46 |
| MsG0180005760.01 | MsG0480020522.01 | 0.823406 | 1.409230e-53 | 1.212170e-50 |
| MsG0180005879.01 | MsG0480020522.01 | 0.848317 | 6.618822e-60 | 1.209901e-56 |
| MsG0180006159.01 | MsG0480020522.01 | 0.817660 | 2.939334e-52 | 2.153641e-49 |
| MsG0180006223.01 | MsG0480020522.01 | 0.803368 | 3.605091e-49 | 1.808291e-46 |
| MsG0280006336.01 | MsG0480020522.01 | 0.809311 | 2.015018e-50 | 1.179058e-47 |
| MsG0280006451.01 | MsG0480020522.01 | 0.806437 | 8.231501e-50 | 4.467636e-47 |
| MsG0280011276.01 | MsG0480020522.01 | 0.814292 | 1.659163e-51 | 1.108934e-48 |
| MsG0280011279.01 | MsG0480020522.01 | 0.824537 | 7.649543e-54 | 6.795160e-51 |
| MsG0280011449.01 | MsG0480020522.01 | 0.802265 | 6.090087e-49 | 2.970108e-46 |
| MsG0380011739.01 | MsG0480020522.01 | 0.818126 | 2.305734e-52 | 1.711158e-49 |
| MsG0380011896.01 | MsG0480020522.01 | 0.808495 | 3.012183e-50 | 1.724849e-47 |
| MsG0380012444.01 | MsG0480020522.01 | 0.806720 | 7.172535e-50 | 3.921266e-47 |
| MsG0380012625.01 | MsG0480020522.01 | 0.815068 | 1.116943e-51 | 7.623068e-49 |
| MsG0380014053.01 | MsG0480020522.01 | 0.807700 | 4.446836e-50 | 2.493709e-47 |
| MsG0380014729.01 | MsG0480020522.01 | 0.818728 | 1.684370e-52 | 1.270927e-49 |
| MsG0480018585.01 | MsG0480020522.01 | -0.800115 | 1.676449e-48 | 7.742133e-46 |
| MsG0480018631.01 | MsG0480020522.01 | 0.827231 | 1.753302e-54 | 1.682487e-51 |
| MsG0480020122.01 | MsG0480020522.01 | 0.819277 | 1.263820e-52 | 9.681640e-50 |
| MsG0480020522.01 | MsG0480021039.01 | 0.804337 | 2.267352e-49 | 1.166002e-46 |
| MsG0480020522.01 | MsG0480021143.01 | 0.807277 | 5.468191e-50 | 3.033050e-47 |
| MsG0480020522.01 | MsG0480021254.01 | 0.800089 | 1.697032e-48 | 7.832243e-46 |
| MsG0480020522.01 | MsG0480021307.01 | 0.849618 | 2.881463e-60 | 5.494666e-57 |
| MsG0480020522.01 | MsG0480021308.01 | 0.826286 | 2.948803e-54 | 2.753495e-51 |
| MsG0480020522.01 | MsG0480021314.01 | 0.882496 | 9.923715e-71 | 6.058257e-67 |
| MsG0480020522.01 | MsG0480021317.01 | 0.868186 | 7.874930e-66 | 2.822985e-62 |
| MsG0480020522.01 | MsG0480021318.01 | 0.818532 | 1.866441e-52 | 1.400744e-49 |
| MsG0480020522.01 | MsG0480021709.01 | 0.828512 | 8.626088e-55 | 8.592663e-52 |
| MsG0480020522.01 | MsG0480022286.01 | 0.834722 | 2.542744e-56 | 3.042806e-53 |
| MsG0480020522.01 | MsG0480022295.01 | 0.833952 | 3.967941e-56 | 4.640958e-53 |
| MsG0480020522.01 | MsG0480022296.01 | 0.840713 | 7.368370e-58 | 1.058519e-54 |
| MsG0480020522.01 | MsG0480022417.01 | 0.826001 | 3.446694e-54 | 3.192495e-51 |
| MsG0480020522.01 | MsG0480022466.01 | 0.830191 | 3.374160e-55 | 3.529875e-52 |
| MsG0480020522.01 | MsG0480022584.01 | 0.809352 | 1.974078e-50 | 1.156453e-47 |
| MsG0480020522.01 | MsG0480022837.01 | 0.815867 | 7.416802e-52 | 5.173878e-49 |
| MsG0480020522.01 | MsG0480023264.01 | 0.903516 | 3.201657e-79 | 4.755461e-75 |
| MsG0480020522.01 | MsG0480023298.01 | 0.918173 | 2.160888e-86 | 6.489513e-82 |
| MsG0480020522.01 | MsG0480023371.01 | 0.822600 | 2.171548e-53 | 1.826215e-50 |
| MsG0480020522.01 | MsG0480023502.01 | 0.819524 | 1.110346e-52 | 8.564989e-50 |
| MsG0480020522.01 | MsG0480023881.01 | 0.810370 | 1.192304e-50 | 7.174067e-48 |
| MsG0480020522.01 | MsG0580024128.01 | 0.802452 | 5.573850e-49 | 2.731303e-46 |
| MsG0480020522.01 | MsG0580024327.01 | 0.804751 | 1.858217e-49 | 9.659409e-47 |
| MsG0480020522.01 | MsG0580024385.01 | 0.832245 | 1.055018e-55 | 1.172389e-52 |
| MsG0480020522.01 | MsG0580024875.01 | 0.813518 | 2.458306e-51 | 1.608902e-48 |
| MsG0480020522.01 | MsG0580024982.01 | 0.847856 | 8.871763e-60 | 1.598017e-56 |
| MsG0480020522.01 | MsG0580025513.01 | 0.812285 | 4.577421e-51 | 2.898606e-48 |
| MsG0480020522.01 | MsG0580025738.01 | 0.849625 | 2.866927e-60 | 5.468282e-57 |
| MsG0480020522.01 | MsG0580025997.01 | 0.825821 | 3.803876e-54 | 3.505239e-51 |
| MsG0480020522.01 | MsG0580026211.01 | 0.811502 | 6.780970e-51 | 4.204734e-48 |
| MsG0480020522.01 | MsG0580027983.01 | 0.834102 | 3.639046e-56 | 4.275211e-53 |
| MsG0480020522.01 | MsG0580028461.01 | 0.804410 | 2.189265e-49 | 1.127946e-46 |
| MsG0480020522.01 | MsG0580028982.01 | 0.802817 | 4.686099e-49 | 2.317879e-46 |
| MsG0480020522.01 | MsG0580029816.01 | 0.827989 | 1.153526e-54 | 1.131716e-51 |
| MsG0480020522.01 | MsG0580030006.01 | 0.826691 | 2.360684e-54 | 2.229880e-51 |
| MsG0480020522.01 | MsG0680030290.01 | 0.814410 | 1.562374e-51 | 1.047633e-48 |
| MsG0480020522.01 | MsG0680030399.01 | 0.809472 | 1.860364e-50 | 1.093258e-47 |
| MsG0480020522.01 | MsG0680032055.01 | 0.828410 | 9.130488e-55 | 9.068307e-52 |
| MsG0480020522.01 | MsG0680033007.01 | 0.820531 | 6.531766e-53 | 5.181453e-50 |
| MsG0480020522.01 | MsG0680034040.01 | 0.806716 | 7.187670e-50 | 3.929054e-47 |
| MsG0480020522.01 | MsG0780036909.01 | 0.828273 | 9.853480e-55 | 9.746719e-52 |
| MsG0480020522.01 | MsG0780037436.01 | 0.813845 | 2.082075e-51 | 1.374757e-48 |
| MsG0480020522.01 | MsG0780038106.01 | 0.826231 | 3.038052e-54 | 2.832469e-51 |
| MsG0480020522.01 | MsG0780038839.01 | 0.847974 | 8.232231e-60 | 1.488307e-56 |
| MsG0480020522.01 | MsG0780039596.01 | 0.804977 | 1.667206e-49 | 8.716740e-47 |
| MsG0480020522.01 | MsG0780039873.01 | 0.860966 | 1.428399e-63 | 3.975846e-60 |
| MsG0480020522.01 | MsG0780040158.01 | 0.842176 | 3.033133e-58 | 4.560925e-55 |
| MsG0480020522.01 | MsG0780040159.01 | 0.813514 | 2.462664e-51 | 1.611583e-48 |
| MsG0480020522.01 | MsG0780040515.01 | -0.830179 | 3.398133e-55 | 3.553565e-52 |
| MsG0480020522.01 | MsG0780040567.01 | 0.800886 | 1.167737e-48 | 5.499734e-46 |
| MsG0480020522.01 | MsG0780040644.01 | 0.811197 | 7.895666e-51 | 4.856448e-48 |
| MsG0480020522.01 | MsG0780040884.01 | 0.833708 | 4.567368e-56 | 5.302112e-53 |
| MsG0480020522.01 | MsG0780040894.01 | 0.803439 | 3.484544e-49 | 1.751092e-46 |
| MsG0480020522.01 | MsG0780041035.01 | 0.805210 | 1.489931e-49 | 7.836557e-47 |
| MsG0480020522.01 | MsG0780041249.01 | 0.807011 | 6.225282e-50 | 3.429087e-47 |
| MsG0480020522.01 | MsG0780041288.01 | 0.819209 | 1.309840e-52 | 1.001546e-49 |
| MsG0480020522.01 | MsG0780041792.01 | 0.804251 | 2.363343e-49 | 1.212628e-46 |
| MsG0480020522.01 | MsG0880042500.01 | 0.809981 | 1.446429e-50 | 8.615307e-48 |
| MsG0480020522.01 | MsG0880042889.01 | 0.841645 | 4.190403e-58 | 6.196844e-55 |
| MsG0480020522.01 | MsG0880042895.01 | 0.847828 | 9.035446e-60 | 1.626057e-56 |
| MsG0480020522.01 | MsG0880042921.01 | 0.848625 | 5.440359e-60 | 1.004393e-56 |
| MsG0480020522.01 | MsG0880043032.01 | 0.824475 | 7.913391e-54 | 7.016346e-51 |
| MsG0480020522.01 | MsG0880043259.01 | 0.812521 | 4.066051e-51 | 2.591002e-48 |
| MsG0480020522.01 | MsG0880043918.01 | 0.837886 | 3.988403e-57 | 5.252920e-54 |
| MsG0480020522.01 | MsG0880044352.01 | 0.824252 | 8.927379e-54 | 7.865127e-51 |
| MsG0480020522.01 | MsG0880045073.01 | 0.806722 | 7.166952e-50 | 3.918400e-47 |
| MsG0480020522.01 | MsG0880045252.01 | 0.845594 | 3.683939e-59 | 6.169810e-56 |
| MsG0480020522.01 | MsG0880045412.01 | 0.805585 | 1.243102e-49 | 6.601675e-47 |
| MsG0480020522.01 | MsG0880045778.01 | 0.803205 | 3.895991e-49 | 1.946103e-46 |
| MsG0480020522.01 | MsG0880046104.01 | 0.814472 | 1.514239e-51 | 1.017029e-48 |
| MsG0480020522.01 | MsG0880046462.01 | 0.805565 | 1.255448e-49 | 6.663489e-47 |
| MsG0480020522.01 | MsG0880046854.01 | 0.836159 | 1.101639e-56 | 1.376775e-53 |
| MsG0480020522.01 | MsG0880046866.01 | 0.830746 | 2.468433e-55 | 2.624374e-52 |
| MsG0480020522.01 | MsG0880047567.01 | 0.849472 | 3.163336e-60 | 6.003107e-57 |
| MsG0480020522.01 | MsG0880047616.01 | 0.816792 | 4.607179e-52 | 3.296306e-49 |
| MsG0480020522.01 | MsG0880047628.01 | 0.804652 | 1.949649e-49 | 1.010797e-46 |
| MsG0480020522.01 | MsG0880047661.01 | 0.835170 | 1.961173e-56 | 2.378989e-53 |
| MsG0480020522.01 | MsG0880047669.01 | 0.835783 | 1.372048e-56 | 1.695263e-53 |
| MsG0480020522.01 | MsG0880047670.01 | 0.813312 | 2.727759e-51 | 1.775266e-48 |
| MsG0380015081.01 | MsG0480020522.01 | 0.802222 | 6.213832e-49 | 3.027098e-46 |
| MsG0380015546.01 | MsG0480020522.01 | 0.834277 | 3.289676e-56 | 3.885291e-53 |
| MsG0380015883.01 | MsG0480020522.01 | 0.813835 | 2.093536e-51 | 1.381914e-48 |
| MsG0380015900.01 | MsG0480020522.01 | 0.814623 | 1.401938e-51 | 9.454133e-49 |
| MsG0380016478.01 | MsG0480020522.01 | 0.807996 | 3.847964e-50 | 2.174659e-47 |
| MsG0380016896.01 | MsG0480020522.01 | 0.824172 | 9.322031e-54 | 8.194713e-51 |
| MsG0380016957.01 | MsG0480020522.01 | 0.813492 | 2.490479e-51 | 1.628860e-48 |
| MsG0380016960.01 | MsG0480020522.01 | 0.802309 | 5.964841e-49 | 2.912425e-46 |
| MsG0380017141.01 | MsG0480020522.01 | 0.875908 | 2.125526e-68 | 1.010391e-64 |
| MsG0380017309.01 | MsG0480020522.01 | 0.809665 | 1.691607e-50 | 9.992361e-48 |
| MsG0380017472.01 | MsG0480020522.01 | 0.816359 | 5.760986e-52 | 4.073341e-49 |
| MsG0380017474.01 | MsG0480020522.01 | 0.816541 | 5.243430e-52 | 3.725768e-49 |
| MsG0380017727.01 | MsG0480020522.01 | 0.854584 | 1.116516e-61 | 2.505721e-58 |
| MsG0380017966.01 | MsG0480020522.01 | 0.819032 | 1.436795e-52 | 1.093355e-49 |
| MsG0380018027.01 | MsG0480020522.01 | 0.804463 | 2.134029e-49 | 1.101014e-46 |
| MsG0480018190.01 | MsG0480020522.01 | 0.820673 | 6.056722e-53 | 4.823380e-50 |
| MsG0280006855.01 | MsG0480020522.01 | -0.820700 | 5.971991e-53 | 4.759599e-50 |
| MsG0280006964.01 | MsG0480020522.01 | 0.805773 | 1.135106e-49 | 6.057529e-47 |
| MsG0280007079.01 | MsG0480020522.01 | 0.800102 | 1.687327e-48 | 7.789817e-46 |
| MsG0280007102.01 | MsG0480020522.01 | 0.801938 | 7.110325e-49 | 3.438805e-46 |
| MsG0280007136.01 | MsG0480020522.01 | 0.808899 | 2.468859e-50 | 1.428708e-47 |
| MsG0280007247.01 | MsG0480020522.01 | 0.818560 | 1.839097e-52 | 1.381250e-49 |
| MsG0280007702.01 | MsG0480020522.01 | 0.800934 | 1.141802e-48 | 5.384026e-46 |
| MsG0280007902.01 | MsG0480020522.01 | 0.816410 | 5.609082e-52 | 3.971422e-49 |
| MsG0280008430.01 | MsG0480020522.01 | 0.812206 | 4.765069e-51 | 3.010819e-48 |
| MsG0280008935.01 | MsG0480020522.01 | 0.815259 | 1.013012e-51 | 6.949773e-49 |
| MsG0280009776.01 | MsG0480020522.01 | 0.807978 | 3.880893e-50 | 2.192257e-47 |
| MsG0280010131.01 | MsG0480020522.01 | 0.829234 | 5.770558e-55 | 5.871115e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020522.01.T01 | MTR_4g052840 | 93.333 | 165 | 10 | 1 | 1 | 165 | 1 | 164 | 3.16e-110 | 314 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020522.01.T01 | AT3G44380 | 65.605 | 157 | 54 | 0 | 5 | 161 | 6 | 162 | 1.03e-67 | 206 |
Find 52 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACTCTCACTTTAAACTTA+TGG | 0.256093 | 4:-42828980 | None:intergenic |
| AGGTGGAATGTTGGGTCTTT+TGG | 0.258820 | 4:-42828621 | None:intergenic |
| AGAACTTCTGCGTCTACAAC+GGG | 0.388729 | 4:-42828681 | None:intergenic |
| CCTTCATTTGTTTCTCATGT+GGG | 0.391323 | 4:+42829889 | MsG0480020522.01.T01:CDS |
| TAGTTGATTGGGGCTATGTT+TGG | 0.400386 | 4:-42828726 | None:intergenic |
| GGCTGAGACCCGGCTTGGAC+AGG | 0.429344 | 4:-42828801 | None:intergenic |
| ACCTTCATTTGTTTCTCATG+TGG | 0.430421 | 4:+42829888 | MsG0480020522.01.T01:CDS |
| GTTGTTGAAGAGTAGTTGAT+TGG | 0.439944 | 4:-42828738 | None:intergenic |
| TTCTACGAAGGTTCTCTCCT+CGG | 0.440988 | 4:+42828771 | MsG0480020522.01.T01:CDS |
| GGTTCAGCTCCTGTCCAAGC+CGG | 0.442027 | 4:+42828792 | MsG0480020522.01.T01:CDS |
| GATGCGGCGGTTGATATTGC+TGG | 0.443475 | 4:+42828933 | MsG0480020522.01.T01:CDS |
| AGCTGGACATCAGCAATAAT+AGG | 0.444898 | 4:+42828555 | MsG0480020522.01.T01:CDS |
| GTTCAGCTCCTGTCCAAGCC+GGG | 0.468167 | 4:+42828793 | MsG0480020522.01.T01:CDS |
| TGGAGATGAGGTGGAATGTT+GGG | 0.469558 | 4:-42828629 | None:intergenic |
| GCGTCTACAACGGGGAGGCT+TGG | 0.496927 | 4:-42828672 | None:intergenic |
| GATTGGGGCTATGTTTGGAT+TGG | 0.499436 | 4:-42828721 | None:intergenic |
| ATGGAGATGAGGTGGAATGT+TGG | 0.499718 | 4:-42828630 | None:intergenic |
| TCGGTGGCTGAGACCCGGCT+TGG | 0.502166 | 4:-42828806 | None:intergenic |
| CGGCCCGGCTTAAAGCTCTT+CGG | 0.502487 | 4:+42828853 | MsG0480020522.01.T01:CDS |
| TTGTTGAAGAGTAGTTGATT+GGG | 0.503825 | 4:-42828737 | None:intergenic |
| AAAAGAGGTCTCAATGGAGC+TGG | 0.510525 | 4:+42828538 | MsG0480020522.01.T01:CDS |
| CGAGCCGAAGAGCTTTAAGC+CGG | 0.512706 | 4:-42828857 | None:intergenic |
| CGAAGAGCTTTAAGCCGGGC+CGG | 0.530658 | 4:-42828852 | None:intergenic |
| ATGTCAGTTACTGAGACTTC+CGG | 0.532229 | 4:+42828833 | MsG0480020522.01.T01:CDS |
| TCGCCACGTCAGACATTACT+CGG | 0.536903 | 4:-42828893 | None:intergenic |
| TGAAGTTGATGGAGATGAGG+TGG | 0.542844 | 4:-42828638 | None:intergenic |
| GATGATGAGTTGGCAGGTGA+AGG | 0.551759 | 4:+42829937 | MsG0480020522.01.T01:CDS |
| AGTTACTGAGACTTCCGGCC+CGG | 0.561478 | 4:+42828838 | MsG0480020522.01.T01:CDS |
| GAGCCGAAGAGCTTTAAGCC+GGG | 0.564963 | 4:-42828856 | None:intergenic |
| TATGCAAGTAGATGATGAGT+TGG | 0.566187 | 4:+42829927 | MsG0480020522.01.T01:CDS |
| TGTTGAAGAGTAGTTGATTG+GGG | 0.588353 | 4:-42828736 | None:intergenic |
| CCCACATGAGAAACAAATGA+AGG | 0.590394 | 4:-42829889 | None:intergenic |
| AAGAACTTCTGCGTCTACAA+CGG | 0.599919 | 4:-42828682 | None:intergenic |
| TGACCTCGGTGGCTGAGACC+CGG | 0.603018 | 4:-42828811 | None:intergenic |
| CAAGTAGATGATGAGTTGGC+AGG | 0.612414 | 4:+42829931 | MsG0480020522.01.T01:CDS |
| GTTTGGATTGGTGACATGAA+CGG | 0.614061 | 4:-42828709 | None:intergenic |
| GCAGGTGAAGGTGAAGGTGA+AGG | 0.632238 | 4:+42829949 | MsG0480020522.01.T01:CDS |
| AAGTGAAGTTGATGGAGATG+AGG | 0.637363 | 4:-42828641 | None:intergenic |
| CTCAGTAACTGACATGACCT+CGG | 0.639739 | 4:-42828825 | None:intergenic |
| CTTGGACAGGAGCTGAACCG+AGG | 0.641650 | 4:-42828788 | None:intergenic |
| ACGTGAGATGGTTCTTGATG+CGG | 0.641818 | 4:+42828917 | MsG0480020522.01.T01:CDS |
| TGAAACTGAAGCAAAAGCTG+AGG | 0.653198 | 4:+42829978 | MsG0480020522.01.T01:CDS |
| TGAGATGGTTCTTGATGCGG+CGG | 0.662664 | 4:+42828920 | MsG0480020522.01.T01:CDS |
| GAGCCGAGTAATGTCTGACG+TGG | 0.684373 | 4:+42828890 | MsG0480020522.01.T01:CDS |
| TGACGTGGCGAAACGTGAGA+TGG | 0.689628 | 4:+42828905 | MsG0480020522.01.T01:CDS |
| GTCCCACCACAAAACTCTAG+CGG | 0.692849 | 4:-42828958 | None:intergenic |
| ACCTTCGTAGAAAATTGACA+TGG | 0.695121 | 4:-42828760 | None:intergenic |
| GAGTTGGCAGGTGAAGGTGA+AGG | 0.709725 | 4:+42829943 | MsG0480020522.01.T01:CDS |
| AAGCCGGGTCTCAGCCACCG+AGG | 0.724149 | 4:+42828808 | MsG0480020522.01.T01:CDS |
| CTTCTGCGTCTACAACGGGG+AGG | 0.732058 | 4:-42828677 | None:intergenic |
| AGTAACTGACATGACCTCGG+TGG | 0.742599 | 4:-42828822 | None:intergenic |
| GAACTTCTGCGTCTACAACG+GGG | 0.764589 | 4:-42828680 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CGAAATAATCAAATAAATTA+TGG | - | Chr4:42829375-42829394 | None:intergenic | 15.0% |
| ! | AATCTTCAACACAACAAATA+CGG | - | Chr4:42829818-42829837 | None:intergenic | 25.0% |
| ! | ACTCAAAATCTAGGTTAATA+CGG | - | Chr4:42829723-42829742 | None:intergenic | 25.0% |
| ! | AGCACAAAACATTAAAAACA+AGG | - | Chr4:42829235-42829254 | None:intergenic | 25.0% |
| ! | CAAATTAAGCTTAGAAAAAG+TGG | + | Chr4:42829120-42829139 | MsG0480020522.01.T01:intron | 25.0% |
| ! | TAACTCTCACTTTAAACTTA+TGG | - | Chr4:42828983-42829002 | None:intergenic | 25.0% |
| ! | TCAATAACATCAAGAAAAAC+AGG | - | Chr4:42829026-42829045 | None:intergenic | 25.0% |
| !! | TGGGTTACATATGTATTTTT+AGG | + | Chr4:42829584-42829603 | MsG0480020522.01.T01:intron | 25.0% |
| !! | TTTTCTTGATGTTATTGATC+AGG | + | Chr4:42829028-42829047 | MsG0480020522.01.T01:intron | 25.0% |
| AATGTGATCACTCAAAATCT+AGG | - | Chr4:42829732-42829751 | None:intergenic | 30.0% | |
| CTCAAAATCTAGGTTAATAC+GGG | - | Chr4:42829722-42829741 | None:intergenic | 30.0% | |
| GACGAAACTCTTTAATTCAT+GGG | + | Chr4:42829677-42829696 | MsG0480020522.01.T01:intron | 30.0% | |
| TGACGAAACTCTTTAATTCA+TGG | + | Chr4:42829676-42829695 | MsG0480020522.01.T01:intron | 30.0% | |
| TTGTTGAAGAGTAGTTGATT+GGG | - | Chr4:42828740-42828759 | None:intergenic | 30.0% | |
| ! | ATTTGTTAGGTTTGGGAAAT+TGG | + | Chr4:42829147-42829166 | MsG0480020522.01.T01:intron | 30.0% |
| !! | AAAAAGTGGTGTGATTTGTT+AGG | + | Chr4:42829134-42829153 | MsG0480020522.01.T01:intron | 30.0% |
| !! | TTTTAAGGAAGTGAAGTTGA+TGG | - | Chr4:42828652-42828671 | None:intergenic | 30.0% |
| AAGAGAAAAAGAGGTCTCAA+TGG | + | Chr4:42828532-42828551 | MsG0480020522.01.T01:CDS | 35.0% | |
| ACCTTCGTAGAAAATTGACA+TGG | - | Chr4:42828763-42828782 | None:intergenic | 35.0% | |
| CAAAATCAAAACGTGACGTT+AGG | - | Chr4:42829180-42829199 | None:intergenic | 35.0% | |
| CATAGATGATTAGTGTTGCA+CGG | + | Chr4:42829757-42829776 | MsG0480020522.01.T01:intron | 35.0% | |
| GCTTCTCAAGTTTGAATTGT+AGG | + | Chr4:42829252-42829271 | MsG0480020522.01.T01:intron | 35.0% | |
| GTAAATGTAGTGTACCTTTG+AGG | + | Chr4:42829617-42829636 | MsG0480020522.01.T01:intron | 35.0% | |
| GTAATTACGTTATGTAGCCT+TGG | + | Chr4:42829487-42829506 | MsG0480020522.01.T01:intron | 35.0% | |
| GTTGTTGAAGAGTAGTTGAT+TGG | - | Chr4:42828741-42828760 | None:intergenic | 35.0% | |
| TATGCAAGTAGATGATGAGT+TGG | + | Chr4:42829927-42829946 | MsG0480020522.01.T01:CDS | 35.0% | |
| TGTTGAAGAGTAGTTGATTG+GGG | - | Chr4:42828739-42828758 | None:intergenic | 35.0% | |
| ! | ACCATGTCAATTTTCTACGA+AGG | + | Chr4:42828759-42828778 | MsG0480020522.01.T01:CDS | 35.0% |
| ! | ACCTTCATTTGTTTCTCATG+TGG | + | Chr4:42829888-42829907 | MsG0480020522.01.T01:CDS | 35.0% |
| ! | CCTTCATTTGTTTCTCATGT+GGG | + | Chr4:42829889-42829908 | MsG0480020522.01.T01:CDS | 35.0% |
| !! | TGGTGTGATTTGTTAGGTTT+GGG | + | Chr4:42829140-42829159 | MsG0480020522.01.T01:intron | 35.0% |
| AAGAACTTCTGCGTCTACAA+CGG | - | Chr4:42828685-42828704 | None:intergenic | 40.0% | |
| ACTGCACAACTTGAACTCTT+TGG | + | Chr4:42829056-42829075 | MsG0480020522.01.T01:intron | 40.0% | |
| AGCTGGACATCAGCAATAAT+AGG | + | Chr4:42828555-42828574 | MsG0480020522.01.T01:CDS | 40.0% | |
| ATCCTACAAATGTCACTCCA+AGG | - | Chr4:42829507-42829526 | None:intergenic | 40.0% | |
| ATGTCAGTTACTGAGACTTC+CGG | + | Chr4:42828833-42828852 | MsG0480020522.01.T01:CDS | 40.0% | |
| CCCACATGAGAAACAAATGA+AGG | - | Chr4:42829892-42829911 | None:intergenic | 40.0% | |
| TAACCCACTTCGATTCTCTA+CGG | - | Chr4:42829571-42829590 | None:intergenic | 40.0% | |
| TAGTTGATTGGGGCTATGTT+TGG | - | Chr4:42828729-42828748 | None:intergenic | 40.0% | |
| TGAAACTGAAGCAAAAGCTG+AGG | + | Chr4:42829978-42829997 | MsG0480020522.01.T01:CDS | 40.0% | |
| !! | AAGTGAAGTTGATGGAGATG+AGG | - | Chr4:42828644-42828663 | None:intergenic | 40.0% |
| !! | GTGGTGTGATTTGTTAGGTT+TGG | + | Chr4:42829139-42829158 | MsG0480020522.01.T01:intron | 40.0% |
| !! | GTTTGGATTGGTGACATGAA+CGG | - | Chr4:42828712-42828731 | None:intergenic | 40.0% |
| AAAAGAGGTCTCAATGGAGC+TGG | + | Chr4:42828538-42828557 | MsG0480020522.01.T01:CDS | 45.0% | |
| ACACCGTAGAGAATCGAAGT+GGG | + | Chr4:42829565-42829584 | MsG0480020522.01.T01:intron | 45.0% | |
| ACGTGAGATGGTTCTTGATG+CGG | + | Chr4:42828917-42828936 | MsG0480020522.01.T01:CDS | 45.0% | |
| AGAACTTCTGCGTCTACAAC+GGG | - | Chr4:42828684-42828703 | None:intergenic | 45.0% | |
| AGCCTTGGAGTGACATTTGT+AGG | + | Chr4:42829502-42829521 | MsG0480020522.01.T01:intron | 45.0% | |
| ATGGAGATGAGGTGGAATGT+TGG | - | Chr4:42828633-42828652 | None:intergenic | 45.0% | |
| CTCAGTAACTGACATGACCT+CGG | - | Chr4:42828828-42828847 | None:intergenic | 45.0% | |
| GATTGGGGCTATGTTTGGAT+TGG | - | Chr4:42828724-42828743 | None:intergenic | 45.0% | |
| TGGAGATGAGGTGGAATGTT+GGG | - | Chr4:42828632-42828651 | None:intergenic | 45.0% | |
| TTCGTCAAATCATCGCCATG+TGG | - | Chr4:42829663-42829682 | None:intergenic | 45.0% | |
| TTCTACGAAGGTTCTCTCCT+CGG | + | Chr4:42828771-42828790 | MsG0480020522.01.T01:CDS | 45.0% | |
| ! | AGGTGGAATGTTGGGTCTTT+TGG | - | Chr4:42828624-42828643 | None:intergenic | 45.0% |
| ! | CAACTTGAACTCTTTGGCTC+AGG | + | Chr4:42829062-42829081 | MsG0480020522.01.T01:intron | 45.0% |
| ! | CAAGTAGATGATGAGTTGGC+AGG | + | Chr4:42829931-42829950 | MsG0480020522.01.T01:CDS | 45.0% |
| ! | CGATTCTCTACGGTGTCAAT+TGG | - | Chr4:42829561-42829580 | None:intergenic | 45.0% |
| ! | CTCTACGGTGTCAATTGGAT+GGG | - | Chr4:42829556-42829575 | None:intergenic | 45.0% |
| ! | TCTCTACGGTGTCAATTGGA+TGG | - | Chr4:42829557-42829576 | None:intergenic | 45.0% |
| ! | TTGAACTCTTTGGCTCAGGT+CGG | + | Chr4:42829066-42829085 | MsG0480020522.01.T01:intron | 45.0% |
| !! | AGTGTACCTTTGAGGCGTTT+CGG | + | Chr4:42829625-42829644 | MsG0480020522.01.T01:intron | 45.0% |
| !! | TGAAGTTGATGGAGATGAGG+TGG | - | Chr4:42828641-42828660 | None:intergenic | 45.0% |
| AGTAACTGACATGACCTCGG+TGG | - | Chr4:42828825-42828844 | None:intergenic | 50.0% | |
| GAACTTCTGCGTCTACAACG+GGG | - | Chr4:42828683-42828702 | None:intergenic | 50.0% | |
| GACACCGTAGAGAATCGAAG+TGG | + | Chr4:42829564-42829583 | MsG0480020522.01.T01:intron | 50.0% | |
| GTCCCACCACAAAACTCTAG+CGG | - | Chr4:42828961-42828980 | None:intergenic | 50.0% | |
| TCGCCACGTCAGACATTACT+CGG | - | Chr4:42828896-42828915 | None:intergenic | 50.0% | |
| TGAGATGGTTCTTGATGCGG+CGG | + | Chr4:42828920-42828939 | MsG0480020522.01.T01:CDS | 50.0% | |
| ! | GATGATGAGTTGGCAGGTGA+AGG | + | Chr4:42829937-42829956 | MsG0480020522.01.T01:CDS | 50.0% |
| !! | CACCGCTAGAGTTTTGTGGT+GGG | + | Chr4:42828956-42828975 | MsG0480020522.01.T01:CDS | 50.0% |
| !!! | AACGGGGAGGCTTGGTTTTA+AGG | - | Chr4:42828667-42828686 | None:intergenic | 50.0% |
| AGTTACTGAGACTTCCGGCC+CGG | + | Chr4:42828838-42828857 | MsG0480020522.01.T01:CDS | 55.0% | |
| ATCGAGCCGAAACGCCTCAA+AGG | - | Chr4:42829634-42829653 | None:intergenic | 55.0% | |
| CGGCTCGATAATGAGCCACA+TGG | + | Chr4:42829645-42829664 | MsG0480020522.01.T01:intron | 55.0% | |
| GAGCCGAGTAATGTCTGACG+TGG | + | Chr4:42828890-42828909 | MsG0480020522.01.T01:CDS | 55.0% | |
| GAGTTGGCAGGTGAAGGTGA+AGG | + | Chr4:42829943-42829962 | MsG0480020522.01.T01:CDS | 55.0% | |
| GCAGGTGAAGGTGAAGGTGA+AGG | + | Chr4:42829949-42829968 | MsG0480020522.01.T01:CDS | 55.0% | |
| TGACGTGGCGAAACGTGAGA+TGG | + | Chr4:42828905-42828924 | MsG0480020522.01.T01:CDS | 55.0% | |
| ! | CTGGCACCGCTAGAGTTTTG+TGG | + | Chr4:42828952-42828971 | MsG0480020522.01.T01:CDS | 55.0% |
| ! | GATGCGGCGGTTGATATTGC+TGG | + | Chr4:42828933-42828952 | MsG0480020522.01.T01:CDS | 55.0% |
| !! | CGAGCCGAAGAGCTTTAAGC+CGG | - | Chr4:42828860-42828879 | None:intergenic | 55.0% |
| !! | GAGCCGAAGAGCTTTAAGCC+GGG | - | Chr4:42828859-42828878 | None:intergenic | 55.0% |
| !! | GCACCGCTAGAGTTTTGTGG+TGG | + | Chr4:42828955-42828974 | MsG0480020522.01.T01:CDS | 55.0% |
| CGGCCCGGCTTAAAGCTCTT+CGG | + | Chr4:42828853-42828872 | MsG0480020522.01.T01:CDS | 60.0% | |
| CTTCTGCGTCTACAACGGGG+AGG | - | Chr4:42828680-42828699 | None:intergenic | 60.0% | |
| CTTGGACAGGAGCTGAACCG+AGG | - | Chr4:42828791-42828810 | None:intergenic | 60.0% | |
| GGTTCAGCTCCTGTCCAAGC+CGG | + | Chr4:42828792-42828811 | MsG0480020522.01.T01:CDS | 60.0% | |
| GTTCAGCTCCTGTCCAAGCC+GGG | + | Chr4:42828793-42828812 | MsG0480020522.01.T01:CDS | 60.0% | |
| !! | CGAAGAGCTTTAAGCCGGGC+CGG | - | Chr4:42828855-42828874 | None:intergenic | 60.0% |
| GCGTCTACAACGGGGAGGCT+TGG | - | Chr4:42828675-42828694 | None:intergenic | 65.0% | |
| TGACCTCGGTGGCTGAGACC+CGG | - | Chr4:42828814-42828833 | None:intergenic | 65.0% | |
| AAGCCGGGTCTCAGCCACCG+AGG | + | Chr4:42828808-42828827 | MsG0480020522.01.T01:CDS | 70.0% | |
| TCGGTGGCTGAGACCCGGCT+TGG | - | Chr4:42828809-42828828 | None:intergenic | 70.0% | |
| !! | GGCTGAGACCCGGCTTGGAC+AGG | - | Chr4:42828804-42828823 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 42828528 | 42830005 | 42828528 | ID=MsG0480020522.01;Name=MsG0480020522.01 |
| Chr4 | mRNA | 42828528 | 42830005 | 42828528 | ID=MsG0480020522.01.T01;Parent=MsG0480020522.01;Name=MsG0480020522.01.T01;_AED=0.43;_eAED=0.53;_QI=0|0|0|1|1|1|2|0|211 |
| Chr4 | exon | 42828528 | 42828992 | 42828528 | ID=MsG0480020522.01.T01:exon:4608;Parent=MsG0480020522.01.T01 |
| Chr4 | exon | 42829835 | 42830005 | 42829835 | ID=MsG0480020522.01.T01:exon:4609;Parent=MsG0480020522.01.T01 |
| Chr4 | CDS | 42828528 | 42828992 | 42828528 | ID=MsG0480020522.01.T01:cds;Parent=MsG0480020522.01.T01 |
| Chr4 | CDS | 42829835 | 42830005 | 42829835 | ID=MsG0480020522.01.T01:cds;Parent=MsG0480020522.01.T01 |
| Gene Sequence |
| Protein sequence |
