Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044731.01.T01 | XP_003628372.1 | 75 | 200 | 39 | 1 | 1 | 189 | 162 | 361 | 8.26E-97 | 295 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044731.01.T01 | C7B178 | 37.755 | 98 | 61 | 0 | 83 | 180 | 386 | 483 | 2.84E-14 | 73.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044731.01.T01 | G7LAY3 | 75.000 | 200 | 39 | 1 | 1 | 189 | 162 | 361 | 3.94e-97 | 295 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005334.01 | MsG0880044731.01 | 0.817235 | 3.663217e-52 | 2.652898e-49 |
| MsG0780041016.01 | MsG0880044731.01 | 0.806676 | 7.327949e-50 | 4.001485e-47 |
| MsG0480022781.01 | MsG0880044731.01 | -0.802679 | 5.002645e-49 | 2.465613e-46 |
| MsG0480023009.01 | MsG0880044731.01 | 0.813706 | 2.234545e-51 | 1.469933e-48 |
| MsG0380016778.01 | MsG0880044731.01 | 0.808935 | 2.424842e-50 | 1.404626e-47 |
| MsG0780036396.01 | MsG0880044731.01 | -0.808354 | 3.227511e-50 | 1.841463e-47 |
| MsG0780037093.01 | MsG0880044731.01 | 0.819122 | 1.370631e-52 | 1.045532e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044731.01.T01 | MTR_8g056020 | 75.000 | 200 | 39 | 1 | 1 | 189 | 162 | 361 | 1.00e-100 | 295 |
| MsG0880044731.01.T01 | MTR_6g027840 | 36.735 | 98 | 62 | 0 | 83 | 180 | 390 | 487 | 2.36e-14 | 70.9 |
| MsG0880044731.01.T01 | MTR_3g088410 | 36.275 | 102 | 65 | 0 | 83 | 184 | 46 | 147 | 2.20e-11 | 59.7 |
| MsG0880044731.01.T01 | MTR_1g062770 | 37.624 | 101 | 56 | 1 | 90 | 183 | 71 | 171 | 2.60e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880044731.01.T01 | AT2G03430 | 37.624 | 101 | 60 | 1 | 83 | 180 | 40 | 140 | 1.01e-13 | 67.8 |
Find 55 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATGCCGCGGCATTTAA+AGG | 0.179032 | 8:+48401151 | MsG0880044731.01.T01:CDS |
| TTTGTATATGGCTAGAGTTT+GGG | 0.273449 | 8:+48401348 | MsG0880044731.01.T01:CDS |
| CTTTGTATATGGCTAGAGTT+TGG | 0.305626 | 8:+48401347 | MsG0880044731.01.T01:CDS |
| ACAGCTCAAATTGTTTGTTA+TGG | 0.332276 | 8:-48401470 | None:intergenic |
| TTCAACTATCGTGATCATTA+TGG | 0.335718 | 8:+48401118 | MsG0880044731.01.T01:CDS |
| AAATTGTTTGTTATGGTTGA+TGG | 0.357177 | 8:-48401463 | None:intergenic |
| GGGTGTCACACCTTTGTATA+TGG | 0.371884 | 8:+48401336 | MsG0880044731.01.T01:CDS |
| GGCTAGAGTTTGGGGTTATG+AGG | 0.372135 | 8:+48401357 | MsG0880044731.01.T01:CDS |
| CCCTACCTCACTAAGCATTA+AGG | 0.379918 | 8:-48401188 | None:intergenic |
| GTTGGCTTACCCAGATGATA+TGG | 0.384641 | 8:+48400220 | MsG0880044731.01.T01:CDS |
| TTCATGCCGCGGCATTTAAA+GGG | 0.388722 | 8:+48401152 | MsG0880044731.01.T01:CDS |
| AGAATATGAACCATATCATC+TGG | 0.392290 | 8:-48400230 | None:intergenic |
| ACATGGAAAATTGATCTTAG+AGG | 0.398717 | 8:+48401040 | MsG0880044731.01.T01:CDS |
| TGTGACACCCCTCTTGTTCA+TGG | 0.404169 | 8:-48401323 | None:intergenic |
| GAACTCTCAATCCAGCTTCA+AGG | 0.419888 | 8:-48400253 | None:intergenic |
| GCCTTAATGCTTAGTGAGGT+AGG | 0.437987 | 8:+48401187 | MsG0880044731.01.T01:CDS |
| GTTCATATTCTCCTTGAAGC+TGG | 0.439212 | 8:+48400242 | MsG0880044731.01.T01:CDS |
| TCTACGAGTTGCTACGGAGT+TGG | 0.441897 | 8:+48400202 | MsG0880044731.01.T01:CDS |
| CCGCGGCATGAAGCGCTGTT+AGG | 0.451448 | 8:-48401141 | None:intergenic |
| CCCTCTTGTTCATGGCATTG+AGG | 0.451645 | 8:-48401315 | None:intergenic |
| CTTCGAGGCATGGAAATGTA+AGG | 0.462915 | 8:+48401063 | MsG0880044731.01.T01:CDS |
| TTTGTGTTAGGTGGAGGATG+TGG | 0.465315 | 8:+48401016 | MsG0880044731.01.T01:intron |
| ATCTTAGAGGCTTCGAGGCA+TGG | 0.471004 | 8:+48401053 | MsG0880044731.01.T01:CDS |
| GTTGGATTCGATAAGTTCAA+TGG | 0.476237 | 8:+48400284 | MsG0880044731.01.T01:CDS |
| TTGAGAGTTCATGAAGCGGT+TGG | 0.494506 | 8:+48400266 | MsG0880044731.01.T01:CDS |
| CCTTAATGCTTAGTGAGGTA+GGG | 0.511752 | 8:+48401188 | MsG0880044731.01.T01:CDS |
| TGGTTTGGATATGTCGTTGA+AGG | 0.520263 | 8:+48400166 | None:intergenic |
| AGGTAATGTGCCCTTGCATA+TGG | 0.523817 | 8:+48401237 | MsG0880044731.01.T01:CDS |
| AATTGATCTTAGAGGCTTCG+AGG | 0.531868 | 8:+48401048 | MsG0880044731.01.T01:CDS |
| TTCTGATGATGCACTTGTGG+TGG | 0.537286 | 8:+48400307 | MsG0880044731.01.T01:CDS |
| TTGTATATGGCTAGAGTTTG+GGG | 0.538238 | 8:+48401349 | MsG0880044731.01.T01:CDS |
| TCTGATGATGCACTTGTGGT+GGG | 0.549538 | 8:+48400308 | MsG0880044731.01.T01:CDS |
| TAGGTGGAGGATGTGGAACA+TGG | 0.560491 | 8:+48401023 | MsG0880044731.01.T01:intron |
| AATGCTTAGTGAGGTAGGGT+TGG | 0.564153 | 8:+48401192 | MsG0880044731.01.T01:CDS |
| TTAGAATGTGAAGATGATGA+AGG | 0.575491 | 8:+48401217 | MsG0880044731.01.T01:CDS |
| TGGTTCTGATGATGCACTTG+TGG | 0.577931 | 8:+48400304 | MsG0880044731.01.T01:CDS |
| ACTCTCCACTGCCATATGCA+AGG | 0.581926 | 8:-48401248 | None:intergenic |
| TGTGCCCTTGCATATGGCAG+TGG | 0.592007 | 8:+48401243 | MsG0880044731.01.T01:CDS |
| CCTAACAGCGCTTCATGCCG+CGG | 0.596465 | 8:+48401141 | MsG0880044731.01.T01:CDS |
| TTGCATATGGCAGTGGAGAG+TGG | 0.598353 | 8:+48401250 | MsG0880044731.01.T01:CDS |
| ACCTCAATGCCATGAACAAG+AGG | 0.599089 | 8:+48401314 | MsG0880044731.01.T01:CDS |
| CTTGTGCCCTTTAAATGCCG+CGG | 0.601819 | 8:-48401158 | None:intergenic |
| GTCGCTTCTACGAGTTGCTA+CGG | 0.607205 | 8:+48400196 | MsG0880044731.01.T01:CDS |
| CTCTCCACTGCCATATGCAA+GGG | 0.616327 | 8:-48401247 | None:intergenic |
| TTCGAGGCATGGAAATGTAA+GGG | 0.616896 | 8:+48401064 | MsG0880044731.01.T01:CDS |
| GAATATGAACCATATCATCT+GGG | 0.617341 | 8:-48400229 | None:intergenic |
| TGTCAGTTGCTTATGAGCAA+AGG | 0.622180 | 8:+48401385 | MsG0880044731.01.T01:CDS |
| CCTCAATGCCATGAACAAGA+GGG | 0.626433 | 8:+48401315 | MsG0880044731.01.T01:CDS |
| ATTTAAAGGGCACAAGAATG+TGG | 0.632752 | 8:+48401165 | MsG0880044731.01.T01:CDS |
| TGAGCTGTGAAAATAGATGA+TGG | 0.648677 | 8:+48401485 | MsG0880044731.01.T01:three_prime_UTR |
| CTCAATGCCATGAACAAGAG+GGG | 0.659679 | 8:+48401316 | MsG0880044731.01.T01:CDS |
| TGTGGCCTTAATGCTTAGTG+AGG | 0.669020 | 8:+48401183 | MsG0880044731.01.T01:CDS |
| TGGATTGAGAGTTCATGAAG+CGG | 0.683200 | 8:+48400262 | MsG0880044731.01.T01:CDS |
| CAAACTCTAGCCATATACAA+AGG | 0.685864 | 8:-48401346 | None:intergenic |
| GTCGAATTACTGTTACGACG+AGG | 0.776138 | 8:+48401089 | MsG0880044731.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTATAGTTTTAAAATTGTT+AGG | - | Chr8:48400935-48400954 | None:intergenic | 15.0% |
| !!! | ATTTCTTTTTTTTTTGTGTT+AGG | + | Chr8:48401004-48401023 | MsG0880044731.01.T01:intron | 15.0% |
| !!! | CAATTTTAAAACTATAGTTA+AGG | + | Chr8:48400937-48400956 | MsG0880044731.01.T01:intron | 15.0% |
| !!! | CTATCAAATACATAATTTTA+AGG | + | Chr8:48401534-48401553 | MsG0880044731.01.T01:three_prime_UTR | 15.0% |
| !! | CAAAAAAATATTGTAATGTG+TGG | + | Chr8:48400443-48400462 | MsG0880044731.01.T01:intron | 20.0% |
| !! | CAAATTTACATTATGCTTTA+TGG | + | Chr8:48400648-48400667 | MsG0880044731.01.T01:intron | 20.0% |
| !! | TTTGAATAACATTGAATTCT+AGG | + | Chr8:48400884-48400903 | MsG0880044731.01.T01:intron | 20.0% |
| !!! | ATATGTTGACATGTTTTAAA+AGG | + | Chr8:48400709-48400728 | MsG0880044731.01.T01:intron | 20.0% |
| !!! | CACATCAATTTTTATCTTAA+CGG | - | Chr8:48400840-48400859 | None:intergenic | 20.0% |
| !!! | TTTGTTTGTTTTCTCTAAAA+CGG | - | Chr8:48400394-48400413 | None:intergenic | 20.0% |
| !!! | TTTTAGAGAAAACAAACAAA+CGG | + | Chr8:48400394-48400413 | MsG0880044731.01.T01:intron | 20.0% |
| ! | ATTTCTTGTGTACTAACTTA+TGG | - | Chr8:48400514-48400533 | None:intergenic | 25.0% |
| ! | GTTGTAAAACTTATGTTTAG+TGG | + | Chr8:48400805-48400824 | MsG0880044731.01.T01:intron | 25.0% |
| ! | TACATTATGCTTTATGGTAT+TGG | + | Chr8:48400654-48400673 | MsG0880044731.01.T01:intron | 25.0% |
| !! | TACTTGAAACTTCAAGTTTA+TGG | + | Chr8:48400564-48400583 | MsG0880044731.01.T01:intron | 25.0% |
| !!! | AAATTGTTTGTTATGGTTGA+TGG | - | Chr8:48401466-48401485 | None:intergenic | 25.0% |
| !!! | TCTTTTTTTTTTGTGTTAGG+TGG | + | Chr8:48401007-48401026 | MsG0880044731.01.T01:intron | 25.0% |
| ACATGGAAAATTGATCTTAG+AGG | + | Chr8:48401040-48401059 | MsG0880044731.01.T01:CDS | 30.0% | |
| AGAATATGAACCATATCATC+TGG | - | Chr8:48400233-48400252 | None:intergenic | 30.0% | |
| CATCTAGCATATCAACAAAA+AGG | - | Chr8:48400984-48401003 | None:intergenic | 30.0% | |
| GAATAACATTGAATTCTAGG+AGG | + | Chr8:48400887-48400906 | MsG0880044731.01.T01:intron | 30.0% | |
| GAATATGAACCATATCATCT+GGG | - | Chr8:48400232-48400251 | None:intergenic | 30.0% | |
| TTAGAATGTGAAGATGATGA+AGG | + | Chr8:48401217-48401236 | MsG0880044731.01.T01:CDS | 30.0% | |
| TTCAACTATCGTGATCATTA+TGG | + | Chr8:48401118-48401137 | MsG0880044731.01.T01:CDS | 30.0% | |
| TTTGTATATGGCTAGAGTTT+GGG | + | Chr8:48401348-48401367 | MsG0880044731.01.T01:CDS | 30.0% | |
| ! | ACAGCTCAAATTGTTTGTTA+TGG | - | Chr8:48401473-48401492 | None:intergenic | 30.0% |
| !! | AGTTAGTGTTTTTGTTGACA+AGG | + | Chr8:48401285-48401304 | MsG0880044731.01.T01:CDS | 30.0% |
| !! | GTTAGTGTTTTTGTTGACAA+GGG | + | Chr8:48401286-48401305 | MsG0880044731.01.T01:CDS | 30.0% |
| !!! | TTTTTTTTTGTGTTAGGTGG+AGG | + | Chr8:48401010-48401029 | MsG0880044731.01.T01:intron | 30.0% |
| CAAACTCTAGCCATATACAA+AGG | - | Chr8:48401349-48401368 | None:intergenic | 35.0% | |
| CTTTGTATATGGCTAGAGTT+TGG | + | Chr8:48401347-48401366 | MsG0880044731.01.T01:CDS | 35.0% | |
| GGTAGTAAACTTCAAATGTG+CGG | + | Chr8:48400585-48400604 | MsG0880044731.01.T01:intron | 35.0% | |
| GTTGGATTCGATAAGTTCAA+TGG | + | Chr8:48400284-48400303 | MsG0880044731.01.T01:CDS | 35.0% | |
| TGAGCTGTGAAAATAGATGA+TGG | + | Chr8:48401485-48401504 | MsG0880044731.01.T01:three_prime_UTR | 35.0% | |
| TTGTATATGGCTAGAGTTTG+GGG | + | Chr8:48401349-48401368 | MsG0880044731.01.T01:CDS | 35.0% | |
| ! | ATTTAAAGGGCACAAGAATG+TGG | + | Chr8:48401165-48401184 | MsG0880044731.01.T01:CDS | 35.0% |
| AATTGATCTTAGAGGCTTCG+AGG | + | Chr8:48401048-48401067 | MsG0880044731.01.T01:CDS | 40.0% | |
| CCTTAATGCTTAGTGAGGTA+GGG | + | Chr8:48401188-48401207 | MsG0880044731.01.T01:CDS | 40.0% | |
| GTTCATATTCTCCTTGAAGC+TGG | + | Chr8:48400242-48400261 | MsG0880044731.01.T01:CDS | 40.0% | |
| TGGATTGAGAGTTCATGAAG+CGG | + | Chr8:48400262-48400281 | MsG0880044731.01.T01:CDS | 40.0% | |
| TGTCAGTTGCTTATGAGCAA+AGG | + | Chr8:48401385-48401404 | MsG0880044731.01.T01:CDS | 40.0% | |
| TTCGAGGCATGGAAATGTAA+GGG | + | Chr8:48401064-48401083 | MsG0880044731.01.T01:CDS | 40.0% | |
| AATGCTTAGTGAGGTAGGGT+TGG | + | Chr8:48401192-48401211 | MsG0880044731.01.T01:CDS | 45.0% | |
| ACCTCAATGCCATGAACAAG+AGG | + | Chr8:48401314-48401333 | MsG0880044731.01.T01:CDS | 45.0% | |
| AGGTAATGTGCCCTTGCATA+TGG | + | Chr8:48401237-48401256 | MsG0880044731.01.T01:CDS | 45.0% | |
| CCCTACCTCACTAAGCATTA+AGG | - | Chr8:48401191-48401210 | None:intergenic | 45.0% | |
| CCTCAATGCCATGAACAAGA+GGG | + | Chr8:48401315-48401334 | MsG0880044731.01.T01:CDS | 45.0% | |
| CTCAATGCCATGAACAAGAG+GGG | + | Chr8:48401316-48401335 | MsG0880044731.01.T01:CDS | 45.0% | |
| CTTCGAGGCATGGAAATGTA+AGG | + | Chr8:48401063-48401082 | MsG0880044731.01.T01:CDS | 45.0% | |
| GAACTCTCAATCCAGCTTCA+AGG | - | Chr8:48400256-48400275 | None:intergenic | 45.0% | |
| GCCTTAATGCTTAGTGAGGT+AGG | + | Chr8:48401187-48401206 | MsG0880044731.01.T01:CDS | 45.0% | |
| GTCGAATTACTGTTACGACG+AGG | + | Chr8:48401089-48401108 | MsG0880044731.01.T01:CDS | 45.0% | |
| TTCATGCCGCGGCATTTAAA+GGG | + | Chr8:48401152-48401171 | MsG0880044731.01.T01:CDS | 45.0% | |
| TTGAGAGTTCATGAAGCGGT+TGG | + | Chr8:48400266-48400285 | MsG0880044731.01.T01:CDS | 45.0% | |
| TTTGTGTTAGGTGGAGGATG+TGG | + | Chr8:48401016-48401035 | MsG0880044731.01.T01:intron | 45.0% | |
| ! | GGGTGTCACACCTTTGTATA+TGG | + | Chr8:48401336-48401355 | MsG0880044731.01.T01:CDS | 45.0% |
| ! | GTTGGCTTACCCAGATGATA+TGG | + | Chr8:48400220-48400239 | MsG0880044731.01.T01:CDS | 45.0% |
| ! | TCTGATGATGCACTTGTGGT+GGG | + | Chr8:48400308-48400327 | MsG0880044731.01.T01:CDS | 45.0% |
| ! | TGTGGCCTTAATGCTTAGTG+AGG | + | Chr8:48401183-48401202 | MsG0880044731.01.T01:CDS | 45.0% |
| ! | TTCTGATGATGCACTTGTGG+TGG | + | Chr8:48400307-48400326 | MsG0880044731.01.T01:CDS | 45.0% |
| !! | TGGTTCTGATGATGCACTTG+TGG | + | Chr8:48400304-48400323 | MsG0880044731.01.T01:CDS | 45.0% |
| ACTCTCCACTGCCATATGCA+AGG | - | Chr8:48401251-48401270 | None:intergenic | 50.0% | |
| ATCTTAGAGGCTTCGAGGCA+TGG | + | Chr8:48401053-48401072 | MsG0880044731.01.T01:CDS | 50.0% | |
| CCCTCTTGTTCATGGCATTG+AGG | - | Chr8:48401318-48401337 | None:intergenic | 50.0% | |
| CTCTCCACTGCCATATGCAA+GGG | - | Chr8:48401250-48401269 | None:intergenic | 50.0% | |
| CTTCATGCCGCGGCATTTAA+AGG | + | Chr8:48401151-48401170 | MsG0880044731.01.T01:CDS | 50.0% | |
| CTTGTGCCCTTTAAATGCCG+CGG | - | Chr8:48401161-48401180 | None:intergenic | 50.0% | |
| GTCGCTTCTACGAGTTGCTA+CGG | + | Chr8:48400196-48400215 | MsG0880044731.01.T01:CDS | 50.0% | |
| TAGGTGGAGGATGTGGAACA+TGG | + | Chr8:48401023-48401042 | MsG0880044731.01.T01:intron | 50.0% | |
| TCTACGAGTTGCTACGGAGT+TGG | + | Chr8:48400202-48400221 | MsG0880044731.01.T01:CDS | 50.0% | |
| TGTGACACCCCTCTTGTTCA+TGG | - | Chr8:48401326-48401345 | None:intergenic | 50.0% | |
| TTGCATATGGCAGTGGAGAG+TGG | + | Chr8:48401250-48401269 | MsG0880044731.01.T01:CDS | 50.0% | |
| ! | GGCTAGAGTTTGGGGTTATG+AGG | + | Chr8:48401357-48401376 | MsG0880044731.01.T01:CDS | 50.0% |
| !! | ATGATGCACTTGTGGTGGGT+GGG | + | Chr8:48400312-48400331 | MsG0880044731.01.T01:intron | 50.0% |
| TGTGCCCTTGCATATGGCAG+TGG | + | Chr8:48401243-48401262 | MsG0880044731.01.T01:CDS | 55.0% | |
| ! | GATGATGCACTTGTGGTGGG+TGG | + | Chr8:48400311-48400330 | MsG0880044731.01.T01:intron | 55.0% |
| CCTAACAGCGCTTCATGCCG+CGG | + | Chr8:48401141-48401160 | MsG0880044731.01.T01:CDS | 60.0% | |
| !! | CCGCGGCATGAAGCGCTGTT+AGG | - | Chr8:48401144-48401163 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 48400176 | 48401594 | 48400176 | ID=MsG0880044731.01;Name=MsG0880044731.01 |
| Chr8 | mRNA | 48400176 | 48401594 | 48400176 | ID=MsG0880044731.01.T01;Parent=MsG0880044731.01;Name=MsG0880044731.01.T01;_AED=0.32;_eAED=0.32;_QI=0|0|0.5|1|1|1|2|153|189 |
| Chr8 | exon | 48400176 | 48400329 | 48400176 | ID=MsG0880044731.01.T01:exon:19982;Parent=MsG0880044731.01.T01 |
| Chr8 | exon | 48401026 | 48401594 | 48401026 | ID=MsG0880044731.01.T01:exon:19983;Parent=MsG0880044731.01.T01 |
| Chr8 | CDS | 48400176 | 48400329 | 48400176 | ID=MsG0880044731.01.T01:cds;Parent=MsG0880044731.01.T01 |
| Chr8 | CDS | 48401026 | 48401441 | 48401026 | ID=MsG0880044731.01.T01:cds;Parent=MsG0880044731.01.T01 |
| Chr8 | three_prime_UTR | 48401442 | 48401594 | 48401442 | ID=MsG0880044731.01.T01:three_prime_utr;Parent=MsG0880044731.01.T01 |
| Gene Sequence |
| Protein sequence |
