AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045614.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045614.01.T01 MTR_8g075310 93.240 429 14 1 1 429 1 414 0.0 816
MsG0880045614.01.T01 MTR_8g062790 83.636 440 65 2 1 440 1 433 0.0 763
MsG0880045614.01.T01 MTR_3g088590 54.887 399 178 1 35 431 73 471 4.13e-163 468
MsG0880045614.01.T01 MTR_2g100510 56.072 387 168 1 47 431 42 428 2.50e-152 440
MsG0880045614.01.T01 MTR_2g097540 53.500 400 184 1 36 433 27 426 4.30e-150 434
MsG0880045614.01.T01 MTR_2g100560 55.297 387 171 1 47 431 47 433 7.29e-149 431
MsG0880045614.01.T01 MTR_2g100520 50.824 425 202 3 12 433 10 430 1.31e-148 430
MsG0880045614.01.T01 MTR_2g097530 54.264 387 175 1 47 431 42 428 5.72e-145 421
MsG0880045614.01.T01 MTR_8g015510 51.449 414 187 5 12 420 12 416 1.35e-142 415
MsG0880045614.01.T01 MTR_3g093030 49.870 385 191 1 49 431 30 414 1.22e-137 401
MsG0880045614.01.T01 MTR_8g063060 59.150 306 95 7 127 429 58 336 3.39e-118 350
MsG0880045614.01.T01 MTR_8g015510 52.525 297 135 4 129 420 1 296 7.12e-101 304
MsG0880045614.01.T01 MTR_2g100550 60.135 148 59 0 47 194 38 185 8.04e-52 184
MsG0880045614.01.T01 MTR_1g028070 46.491 114 59 1 160 271 14 127 9.83e-26 101
MsG0880045614.01.T01 MTR_3g093040 53.763 93 33 1 142 224 1 93 4.15e-22 96.7
MsG0880045614.01.T01 MTR_3g077040 38.636 132 59 2 142 273 1 110 8.42e-19 82.0
MsG0880045614.01.T01 MTR_1205s0010 30.952 168 108 4 255 420 2 163 1.87e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045614.01.T01 AT1G51760 52.133 422 191 4 13 431 9 422 8.09e-153 441
MsG0880045614.01.T01 AT1G44350 50.119 421 194 3 4 418 35 445 5.77e-150 434
MsG0880045614.01.T01 AT5G56660 52.010 398 186 3 36 431 28 422 2.37e-146 424
MsG0880045614.01.T01 AT1G51780 48.837 430 210 3 13 438 6 429 6.61e-143 415
MsG0880045614.01.T01 AT5G54140 48.736 435 205 4 6 437 3 422 3.20e-141 411
MsG0880045614.01.T01 AT5G56650 51.629 399 188 3 35 431 26 421 1.01e-140 410
MsG0880045614.01.T01 AT3G02875 46.667 420 219 2 22 439 18 434 7.60e-137 400
MsG0880045614.01.T01 AT5G56650 56.842 285 119 2 35 317 26 308 3.58e-109 325
MsG0880045614.01.T01 AT3G02875 49.158 297 146 2 22 316 18 311 4.28e-97 295
MsG0880045614.01.T01 AT3G02875 51.969 254 117 2 22 273 18 268 4.59e-86 266

Find 81 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAAACAACCATTGTAAT+TGG 0.155217 8:-61770765 None:intergenic
ACTGGTGTTGTTGCTAAAAT+TGG 0.230227 8:-61770316 MsG0880045614.01.T01:CDS
CATGGATGCTTTGCCAATTC+AGG 0.247866 8:-61770257 MsG0880045614.01.T01:intron
GCGTTGTCTTGATCCAATTT+TGG 0.260435 8:-61768541 MsG0880045614.01.T01:CDS
AAACTTAGAGAGATAAAATA+TGG 0.264275 8:+61770728 None:intergenic
CCTCCAAATGTTACAGAATC+AGG 0.281588 8:+61768258 None:intergenic
GTTGTTGCTAAAATTGGTTC+TGG 0.291878 8:-61770310 MsG0880045614.01.T01:CDS
TTCCAACCAGCCGAGGAAAA+AGG 0.304188 8:-61768741 MsG0880045614.01.T01:CDS
AATTCTTCATATGCAAGTTC+AGG 0.308910 8:+61770409 None:intergenic
TCAGCAGAGCTTGAATTCTT+TGG 0.310519 8:-61767517 MsG0880045614.01.T01:CDS
GATGCCGAAGCAGCCAAAAT+TGG 0.322339 8:+61768528 None:intergenic
TTCATCCATTTCACTGTGTT+GGG 0.326579 8:+61770460 None:intergenic
AGAAATGAAAGACAAATTAA+AGG 0.331922 8:-61768860 MsG0880045614.01.T01:intron
ATCAACAGAGTAAAACCTAT+TGG 0.347944 8:-61770665 MsG0880045614.01.T01:five_prime_UTR
GCAAGTCTTGGATGATTGTT+TGG 0.349735 8:+61770607 None:intergenic
CTTCATCCATTTCACTGTGT+TGG 0.362080 8:+61770459 None:intergenic
GCACATGTGGCGATGCTTCT+TGG 0.363078 8:-61768901 MsG0880045614.01.T01:CDS
AGGACTATGAGCTGAATATA+TGG 0.368562 8:+61767294 None:intergenic
ACCCCTGATTCTGTAACATT+TGG 0.371165 8:-61768261 MsG0880045614.01.T01:CDS
GTTCTAGTTCTAATTCCAAT+AGG 0.396610 8:+61770650 None:intergenic
GATAAACAACCATTGTAATT+GGG 0.398127 8:-61770764 MsG0880045614.01.T01:five_prime_UTR
GTCAACAGGAATTGGTTGAT+TGG 0.400120 8:-61768972 MsG0880045614.01.T01:intron
CAATGGCCTGTGGCTAAAAC+TGG 0.415305 8:-61770334 MsG0880045614.01.T01:CDS
CCTGATTCTGTAACATTTGG+AGG 0.415707 8:-61768258 MsG0880045614.01.T01:CDS
GGTGTTGTTGCGTCGCGTCC+AGG 0.417336 8:-61768630 MsG0880045614.01.T01:CDS
ATTCTCTCATCATTTGTAGT+AGG 0.420169 8:+61767472 None:intergenic
AAAGTATCAAGTATGATTGT+TGG 0.426536 8:-61767433 MsG0880045614.01.T01:CDS
GCTTCAATACACTAAGGAAT+AGG 0.427864 8:-61768209 MsG0880045614.01.T01:CDS
TCAACAGGAATTGGTTGATT+GGG 0.430308 8:-61768971 MsG0880045614.01.T01:intron
TTGATACTTTCCTTGCTAGT+TGG 0.435779 8:+61767446 None:intergenic
GCTTGCAAAGCATTATTTCA+AGG 0.443170 8:-61768497 MsG0880045614.01.T01:CDS
AGGCGTGAGCTTGACAAGTT+AGG 0.445889 8:-61770367 MsG0880045614.01.T01:CDS
ACACACCCAACACAGTGAAA+TGG 0.458134 8:-61770465 MsG0880045614.01.T01:CDS
ATAAGCATGTGGGGTGGAAA+AGG 0.462470 8:+61770563 None:intergenic
TCAAGACAAGTTCTGTCATA+AGG 0.470077 8:-61770387 MsG0880045614.01.T01:CDS
GCAATGATCAATTCAGAGTC+TGG 0.472955 8:-61768294 MsG0880045614.01.T01:CDS
CTCTCATCATTTGTAGTAGG+AGG 0.477042 8:+61767475 None:intergenic
AATAATATAATGAGATATAG+AGG 0.484194 8:-61767134 None:intergenic
GCATGTATTGCAGCGCCAAT+GGG 0.484652 8:+61767244 None:intergenic
ATTCTTCATATGCAAGTTCA+GGG 0.489786 8:+61770410 None:intergenic
GATGAAGCTGTTCTTCCCAT+TGG 0.495132 8:-61767259 MsG0880045614.01.T01:CDS
TTAGCGTCACTGTATCCCTT+AGG 0.496523 8:-61768651 MsG0880045614.01.T01:CDS
AAGCATTATTTCAAGGGAGG+TGG 0.498752 8:-61768490 MsG0880045614.01.T01:CDS
GACTCCACCTGCGAGTCTAA+AGG 0.504759 8:+61768465 None:intergenic
GCTTCAAAGCGAAGATCAAA+GGG 0.510885 8:-61768584 MsG0880045614.01.T01:CDS
CTTGCAAAGCATTATTTCAA+GGG 0.511117 8:-61768496 MsG0880045614.01.T01:CDS
AAATTATCACCAATCGTCAC+AGG 0.513059 8:-61767397 MsG0880045614.01.T01:CDS
TTATTTGTTCAGGTTATCAA+AGG 0.520246 8:-61767562 MsG0880045614.01.T01:intron
TGCATGTATTGCAGCGCCAA+TGG 0.527268 8:+61767243 None:intergenic
TGTTGCTCTTAGAGCTGACA+TGG 0.528134 8:-61770275 MsG0880045614.01.T01:CDS
AGCTTCAAAGCGAAGATCAA+AGG 0.530421 8:-61768585 MsG0880045614.01.T01:CDS
TCTTCACTCCCTGTGACGAT+TGG 0.530485 8:+61767388 None:intergenic
ATGAGTAACAGAGCAAGTCT+TGG 0.532576 8:+61770595 None:intergenic
AGTTAGGTGTTGTTTACCAA+TGG 0.542912 8:-61770351 MsG0880045614.01.T01:CDS
AAAAGAGCTTCAATACACTA+AGG 0.547425 8:-61768215 MsG0880045614.01.T01:CDS
ACGCGACGCAACAACACCTA+AGG 0.548243 8:+61768635 None:intergenic
CTCCAAATGTTACAGAATCA+GGG 0.554065 8:+61768259 None:intergenic
AAGTGTTGAATTTGCAACTG+AGG 0.560490 8:+61770694 None:intergenic
TGTTTGGAGTCATCATGTGA+TGG 0.561200 8:+61770623 None:intergenic
TATTTGTTCAGGTTATCAAA+GGG 0.561602 8:-61767561 MsG0880045614.01.T01:intron
GGTGGATCCTTTAGACTCGC+AGG 0.562046 8:-61768472 MsG0880045614.01.T01:intron
TCAGCTCTAAGAGCAACAAA+AGG 0.582252 8:+61770280 None:intergenic
GCTCTAAGAGCAACAAAAGG+AGG 0.582270 8:+61770283 None:intergenic
GTAGTGGAAGATAAGCATGT+GGG 0.591017 8:+61770553 None:intergenic
AGAGAATCTTCCAACTAGCA+AGG 0.594567 8:-61767456 MsG0880045614.01.T01:CDS
GCAAAGCATTATTTCAAGGG+AGG 0.602378 8:-61768493 MsG0880045614.01.T01:CDS
AATGCTTTGCAAGCTAATCA+CGG 0.603492 8:+61768506 None:intergenic
AGTAGTGGAAGATAAGCATG+TGG 0.617949 8:+61770552 None:intergenic
GATCATAAAAGCAAAGTAGA+CGG 0.622295 8:-61768949 MsG0880045614.01.T01:CDS
CGCGACGCAACAACACCTAA+GGG 0.638716 8:+61768636 None:intergenic
ACTAAGGAATAGGATAGAAG+AGG 0.638854 8:-61768199 MsG0880045614.01.T01:intron
AAATGTGCTTGAAGATGTTG+AGG 0.640356 8:-61768691 MsG0880045614.01.T01:CDS
TGGAAGATAAGCATGTGGGG+TGG 0.642062 8:+61770557 None:intergenic
TCCAAATGTTACAGAATCAG+GGG 0.644080 8:+61768260 None:intergenic
TTGTGCGCACGATGCACATG+TGG 0.644954 8:-61768914 MsG0880045614.01.T01:CDS
AATTATCACCAATCGTCACA+GGG 0.649960 8:-61767396 MsG0880045614.01.T01:CDS
TGTTGTTTACCAATGGCCTG+TGG 0.656833 8:-61770344 MsG0880045614.01.T01:CDS
TTCAAAGCGAAGATCAAAGG+GGG 0.663865 8:-61768582 MsG0880045614.01.T01:CDS
TAGTGGAAGATAAGCATGTG+GGG 0.678703 8:+61770554 None:intergenic
CTTCAAAGCGAAGATCAAAG+GGG 0.736641 8:-61768583 MsG0880045614.01.T01:CDS
ATTGGATCAAGACAACGCTG+CGG 0.767177 8:+61768546 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATACTAGATAAAAAATAT+TGG + Chr8:61768731-61768750 None:intergenic 10.0%
!!! AATATTTTTTATCTAGTATA+TGG - Chr8:61768730-61768749 MsG0880045614.01.T01:CDS 10.0%
!!! ATTAAATTTTCACATTTAAA+TGG + Chr8:61770747-61770766 None:intergenic 10.0%
!! TTGAATATTGTTGAAAAATA+TGG + Chr8:61769852-61769871 None:intergenic 15.0%
!!! AAAATGTTAAAATGAGTTTT+AGG - Chr8:61769075-61769094 MsG0880045614.01.T01:intron 15.0%
!!! AAATGTTAAAATGAGTTTTA+GGG - Chr8:61769076-61769095 MsG0880045614.01.T01:intron 15.0%
!!! ATATTTTTCAACAATATTCA+AGG - Chr8:61769851-61769870 MsG0880045614.01.T01:intron 15.0%
!!! TATATAAAGTTTAATTTTGC+AGG - Chr8:61769570-61769589 MsG0880045614.01.T01:intron 15.0%
!! AAACTTAGAGAGATAAAATA+TGG + Chr8:61767173-61767192 None:intergenic 20.0%
!! AGAAATGAAAGACAAATTAA+AGG - Chr8:61769038-61769057 MsG0880045614.01.T01:intron 20.0%
!! ATTTATTGTTAACAATGCAA+GGG + Chr8:61768884-61768903 None:intergenic 20.0%
!! TATTTATTGTTAACAATGCA+AGG + Chr8:61768885-61768904 None:intergenic 20.0%
!! TTTACAATATAAATCTTCAG+TGG - Chr8:61770045-61770064 MsG0880045614.01.T01:intron 20.0%
!!! AGTATCTTTTCAAAAAATCT+AGG - Chr8:61769980-61769999 MsG0880045614.01.T01:intron 20.0%
!!! GATGTGTACAATTTTTATTA+AGG - Chr8:61767957-61767976 MsG0880045614.01.T01:intron 20.0%
!!! GTACTGTATCTTTTTTTTTT+TGG + Chr8:61768029-61768048 None:intergenic 20.0%
!!! TTTGTTTGATTTATTTGTTC+AGG - Chr8:61770326-61770345 MsG0880045614.01.T01:CDS 20.0%
!!! TTTTTTTGTTTTACCTGAAT+TGG + Chr8:61767657-61767676 None:intergenic 20.0%
! AAGACAAATTAAAGGTAAAG+TGG - Chr8:61769046-61769065 MsG0880045614.01.T01:intron 25.0%
! AATTAAAGGTAAAGTGGAAT+TGG - Chr8:61769052-61769071 MsG0880045614.01.T01:intron 25.0%
! ATTGTAAAACATTGAATCTG+TGG + Chr8:61770162-61770181 None:intergenic 25.0%
! ATTGTTAAACTTGCGTAATT+AGG - Chr8:61768381-61768400 MsG0880045614.01.T01:intron 25.0%
! CATAGTTTCACCAATAATAA+TGG - Chr8:61769896-61769915 MsG0880045614.01.T01:intron 25.0%
! CCATTAATAATCTGAGAAAA+TGG + Chr8:61770198-61770217 None:intergenic 25.0%
! CGCAAGTTTAACAATATTAT+TGG + Chr8:61768376-61768395 None:intergenic 25.0%
! TTAAAAAGTCCCAATTACAA+TGG + Chr8:61767146-61767165 None:intergenic 25.0%
! TTATGAACTAATTGAGAAGT+TGG - Chr8:61770280-61770299 MsG0880045614.01.T01:CDS 25.0%
!! AAAGTATCAAGTATGATTGT+TGG - Chr8:61770465-61770484 MsG0880045614.01.T01:CDS 25.0%
!! CCATTTTCTCAGATTATTAA+TGG - Chr8:61770195-61770214 MsG0880045614.01.T01:intron 25.0%
!! TATTTGTTCAGGTTATCAAA+GGG - Chr8:61770337-61770356 MsG0880045614.01.T01:CDS 25.0%
!! TCTTGGTATTAAGGATTTTT+AGG - Chr8:61768794-61768813 MsG0880045614.01.T01:intron 25.0%
!! TGAAGATTTTGCATTTTACT+TGG - Chr8:61770527-61770546 MsG0880045614.01.T01:CDS 25.0%
!! TTATTTGTTCAGGTTATCAA+AGG - Chr8:61770336-61770355 MsG0880045614.01.T01:CDS 25.0%
!! TTCACCAATAATAATGGTTT+TGG - Chr8:61769902-61769921 MsG0880045614.01.T01:intron 25.0%
!!! TTCTTTTGTTTGATAAGTAG+TGG + Chr8:61767364-61767383 None:intergenic 25.0%
!!! TTTTTTTTTTGGTCGAATTC+TGG + Chr8:61768018-61768037 None:intergenic 25.0%
AAAAACTTCACTCTCATGTT+TGG - Chr8:61767686-61767705 MsG0880045614.01.T01:intron 30.0%
AAAACTTCACTCTCATGTTT+GGG - Chr8:61767687-61767706 MsG0880045614.01.T01:intron 30.0%
AAAAGAGCTTCAATACACTA+AGG - Chr8:61769683-61769702 MsG0880045614.01.T01:intron 30.0%
AACTGGAGAAGAAATTGAAA+AGG - Chr8:61767890-61767909 MsG0880045614.01.T01:intron 30.0%
AATTCTTCATATGCAAGTTC+AGG + Chr8:61767492-61767511 None:intergenic 30.0%
ACAATTACTGGCATTAATTG+CGG + Chr8:61768840-61768859 None:intergenic 30.0%
AGTCTAAACTATAAAGGTCT+TGG - Chr8:61769772-61769791 MsG0880045614.01.T01:intron 30.0%
ATATGTTTGTTCATGTCAAC+AGG - Chr8:61768912-61768931 MsG0880045614.01.T01:CDS 30.0%
ATCAACAGAGTAAAACCTAT+TGG - Chr8:61767233-61767252 MsG0880045614.01.T01:CDS 30.0%
ATTCTCTCATCATTTGTAGT+AGG + Chr8:61770429-61770448 None:intergenic 30.0%
ATTCTTCATATGCAAGTTCA+GGG + Chr8:61767491-61767510 None:intergenic 30.0%
GATCATAAAAGCAAAGTAGA+CGG - Chr8:61768949-61768968 MsG0880045614.01.T01:CDS 30.0%
GGTATAAAAAATGAGAAGAG+TGG - Chr8:61770579-61770598 MsG0880045614.01.T01:CDS 30.0%
GTATCCATGATTCTAAATTG+CGG - Chr8:61768752-61768771 MsG0880045614.01.T01:CDS 30.0%
GTCTCCAAAACCATTATTAT+TGG + Chr8:61769909-61769928 None:intergenic 30.0%
GTTCAATTACGAGCATATTA+GGG - Chr8:61768403-61768422 MsG0880045614.01.T01:intron 30.0%
TAGAAAAAAGCTGAAACTTC+TGG + Chr8:61768051-61768070 None:intergenic 30.0%
TCAATCAAACGAACAATTAC+TGG + Chr8:61768852-61768871 None:intergenic 30.0%
TCACTCCATGTTTGAATTTA+CGG + Chr8:61767823-61767842 None:intergenic 30.0%
TTCAAACTACGATGACAATA+TGG + Chr8:61769747-61769766 None:intergenic 30.0%
! AAATCTAGGATCACTATTCA+CGG - Chr8:61769994-61770013 MsG0880045614.01.T01:intron 30.0%
! AGTTTTTGCCATAATCACTA+TGG - Chr8:61767771-61767790 MsG0880045614.01.T01:intron 30.0%
! CTTATGATTGATTCTGTTTG+TGG - Chr8:61769523-61769542 MsG0880045614.01.T01:intron 30.0%
! CTTGCAAAGCATTATTTCAA+GGG - Chr8:61769402-61769421 MsG0880045614.01.T01:intron 30.0%
!! AATAATCTCACTTTTCAGTG+AGG + Chr8:61767403-61767422 None:intergenic 30.0%
!! GTTCTAGTTCTAATTCCAAT+AGG + Chr8:61767251-61767270 None:intergenic 30.0%
AAATGTGCTTGAAGATGTTG+AGG - Chr8:61769207-61769226 MsG0880045614.01.T01:intron 35.0%
AAATTATCACCAATCGTCAC+AGG - Chr8:61770501-61770520 MsG0880045614.01.T01:CDS 35.0%
AACCGCAGTCTAAACTATAA+AGG - Chr8:61769766-61769785 MsG0880045614.01.T01:intron 35.0%
AAGATACTGTCAAATGCAAC+TGG + Chr8:61769968-61769987 None:intergenic 35.0%
AATGCTTTGCAAGCTAATCA+CGG + Chr8:61769395-61769414 None:intergenic 35.0%
AATGTGTGCCATAGTGATTA+TGG + Chr8:61767782-61767801 None:intergenic 35.0%
AATTATCACCAATCGTCACA+GGG - Chr8:61770502-61770521 MsG0880045614.01.T01:CDS 35.0%
ACTAAGGAATAGGATAGAAG+AGG - Chr8:61769699-61769718 MsG0880045614.01.T01:intron 35.0%
ACTCACCGTAAATTCAAACA+TGG - Chr8:61767815-61767834 MsG0880045614.01.T01:intron 35.0%
AGGACTATGAGCTGAATATA+TGG + Chr8:61770607-61770626 None:intergenic 35.0%
ATTACGAGCATATTAGGGTT+TGG - Chr8:61768408-61768427 MsG0880045614.01.T01:intron 35.0%
CCTAAAAAGAAAAATGAGCC+AGG + Chr8:61770561-61770580 None:intergenic 35.0%
CTAAACTATAAAGGTCTTGG+AGG - Chr8:61769775-61769794 MsG0880045614.01.T01:intron 35.0%
CTCCAAATGTTACAGAATCA+GGG + Chr8:61769642-61769661 None:intergenic 35.0%
CTCGAGATTCAACAAAATCA+CGG + Chr8:61767739-61767758 None:intergenic 35.0%
GACCTTTATAGTTTAGACTG+CGG + Chr8:61769771-61769790 None:intergenic 35.0%
GCTTCAATACACTAAGGAAT+AGG - Chr8:61769689-61769708 MsG0880045614.01.T01:intron 35.0%
GCTTCATCAATGAAAAAGTG+AGG + Chr8:61770627-61770646 None:intergenic 35.0%
GGTTCAATTACGAGCATATT+AGG - Chr8:61768402-61768421 MsG0880045614.01.T01:intron 35.0%
GTTTGAATTTACGGTGAGTT+TGG + Chr8:61767814-61767833 None:intergenic 35.0%
TAATTAGTCCTCCATATGCA+GGG - Chr8:61769118-61769137 MsG0880045614.01.T01:intron 35.0%
TATCCTGCTAAAAATTCACC+TGG + Chr8:61769289-61769308 None:intergenic 35.0%
TCAACAGGAATTGGTTGATT+GGG - Chr8:61768927-61768946 MsG0880045614.01.T01:CDS 35.0%
TCAAGACAAGTTCTGTCATA+AGG - Chr8:61767511-61767530 MsG0880045614.01.T01:CDS 35.0%
TCCAAATGTTACAGAATCAG+GGG + Chr8:61769641-61769660 None:intergenic 35.0%
TTATTGTCGTGATGTAGTTG+TGG + Chr8:61769804-61769823 None:intergenic 35.0%
TTCATCCATTTCACTGTGTT+GGG + Chr8:61767441-61767460 None:intergenic 35.0%
TTGATACTTTCCTTGCTAGT+TGG + Chr8:61770455-61770474 None:intergenic 35.0%
TTGTTCATGTCAACAGGAAT+TGG - Chr8:61768918-61768937 MsG0880045614.01.T01:CDS 35.0%
! ACCCAAAAAATCGCACTTTT+TGG + Chr8:61768325-61768344 None:intergenic 35.0%
! CATCTTCAAGCACATTTTCT+TGG + Chr8:61769204-61769223 None:intergenic 35.0%
! GCTTGCAAAGCATTATTTCA+AGG - Chr8:61769401-61769420 MsG0880045614.01.T01:intron 35.0%
! GTTGTTGCTAAAATTGGTTC+TGG - Chr8:61767588-61767607 MsG0880045614.01.T01:intron 35.0%
!! AAGTGTTGAATTTGCAACTG+AGG + Chr8:61767207-61767226 None:intergenic 35.0%
!! ACTGGTGTTGTTGCTAAAAT+TGG - Chr8:61767582-61767601 MsG0880045614.01.T01:intron 35.0%
!! AGTTAGGTGTTGTTTACCAA+TGG - Chr8:61767547-61767566 MsG0880045614.01.T01:CDS 35.0%
!! CCCAAAAAATCGCACTTTTT+GGG + Chr8:61768324-61768343 None:intergenic 35.0%
!! CCTGGCTCATTTTTCTTTTT+AGG - Chr8:61770558-61770577 MsG0880045614.01.T01:CDS 35.0%
!! GAAGATGTTGAGGCTATTTT+CGG - Chr8:61769217-61769236 MsG0880045614.01.T01:intron 35.0%
!!! ACCCAAAAAGTGCGATTTTT+TGG - Chr8:61768320-61768339 MsG0880045614.01.T01:intron 35.0%
!!! ACTTTTTGGGTTTTTGAAGG+TGG + Chr8:61768311-61768330 None:intergenic 35.0%
!!! CCCAAAAAGTGCGATTTTTT+GGG - Chr8:61768321-61768340 MsG0880045614.01.T01:intron 35.0%
!!! GATGGGTTTTGTTCAAAAAC+AGG + Chr8:61768256-61768275 None:intergenic 35.0%
!!! GGTGAATTTTTAGCAGGATA+TGG - Chr8:61769289-61769308 MsG0880045614.01.T01:intron 35.0%
!!! TTTTACTTGGAAAAAGTGCC+TGG - Chr8:61770540-61770559 MsG0880045614.01.T01:CDS 35.0%
AACTTGCGCAAGGGTAAATT+TGG + Chr8:61768180-61768199 None:intergenic 40.0%
AAGCATTATTTCAAGGGAGG+TGG - Chr8:61769408-61769427 MsG0880045614.01.T01:intron 40.0%
ACCCCTGATTCTGTAACATT+TGG - Chr8:61769637-61769656 MsG0880045614.01.T01:intron 40.0%
AGAAAATGGTGGTTGGAGTT+CGG + Chr8:61768282-61768301 None:intergenic 40.0%
AGAGAATCTTCCAACTAGCA+AGG - Chr8:61770442-61770461 MsG0880045614.01.T01:CDS 40.0%
AGCTTCAAAGCGAAGATCAA+AGG - Chr8:61769313-61769332 MsG0880045614.01.T01:intron 40.0%
ATGAGTAACAGAGCAAGTCT+TGG + Chr8:61767306-61767325 None:intergenic 40.0%
CCTCCAAATGTTACAGAATC+AGG + Chr8:61769643-61769662 None:intergenic 40.0%
CCTGATTCTGTAACATTTGG+AGG - Chr8:61769640-61769659 MsG0880045614.01.T01:intron 40.0%
CTAATTAGTCCTCCATATGC+AGG - Chr8:61769117-61769136 MsG0880045614.01.T01:intron 40.0%
CTCTCATCATTTGTAGTAGG+AGG + Chr8:61770426-61770445 None:intergenic 40.0%
CTTCAAAGCGAAGATCAAAG+GGG - Chr8:61769315-61769334 MsG0880045614.01.T01:intron 40.0%
CTTCATCCATTTCACTGTGT+TGG + Chr8:61767442-61767461 None:intergenic 40.0%
GAGATTCAACAAAATCACGG+TGG + Chr8:61767736-61767755 None:intergenic 40.0%
GCAAGTCTTGGATGATTGTT+TGG + Chr8:61767294-61767313 None:intergenic 40.0%
GCAATGATCAATTCAGAGTC+TGG - Chr8:61769604-61769623 MsG0880045614.01.T01:intron 40.0%
GCTTCAAAGCGAAGATCAAA+GGG - Chr8:61769314-61769333 MsG0880045614.01.T01:intron 40.0%
GTAAATTTGGCATGACAGCA+GGG + Chr8:61768167-61768186 None:intergenic 40.0%
GTCAACAGGAATTGGTTGAT+TGG - Chr8:61768926-61768945 MsG0880045614.01.T01:CDS 40.0%
TAGTGGAAGATAAGCATGTG+GGG + Chr8:61767347-61767366 None:intergenic 40.0%
TCAGCAGAGCTTGAATTCTT+TGG - Chr8:61770381-61770400 MsG0880045614.01.T01:CDS 40.0%
TTCAAAGCGAAGATCAAAGG+GGG - Chr8:61769316-61769335 MsG0880045614.01.T01:intron 40.0%
TTGCAGTTGTGATGCAATTG+TGG + Chr8:61769934-61769953 None:intergenic 40.0%
! AATTTTGCAGGTGCTTTCTG+TGG - Chr8:61769582-61769601 MsG0880045614.01.T01:intron 40.0%
! AGTAGTGGAAGATAAGCATG+TGG + Chr8:61767349-61767368 None:intergenic 40.0%
! CACTTTTCAGTGAGGAAGTT+TGG + Chr8:61767395-61767414 None:intergenic 40.0%
! CAGACCGCAATTTAGAATCA+TGG + Chr8:61768759-61768778 None:intergenic 40.0%
! CATTTGGGGTCTTGGTATTA+AGG - Chr8:61768785-61768804 MsG0880045614.01.T01:intron 40.0%
! GCAAAGCATTATTTCAAGGG+AGG - Chr8:61769405-61769424 MsG0880045614.01.T01:intron 40.0%
! GCGTTGTCTTGATCCAATTT+TGG - Chr8:61769357-61769376 MsG0880045614.01.T01:intron 40.0%
! GTAGTGGAAGATAAGCATGT+GGG + Chr8:61767348-61767367 None:intergenic 40.0%
! TGTTTGGAGTCATCATGTGA+TGG + Chr8:61767278-61767297 None:intergenic 40.0%
!! GTAAACTGCACTTTAGAACC+AGG + Chr8:61770030-61770049 None:intergenic 40.0%
!! TCAGCTCTAAGAGCAACAAA+AGG + Chr8:61767621-61767640 None:intergenic 40.0%
!! TTTGGGTTTTTGAAGGTGGA+GGG + Chr8:61768307-61768326 None:intergenic 40.0%
!!! CGCACTTTTTGGGTTTTTGA+AGG + Chr8:61768314-61768333 None:intergenic 40.0%
!!! TGATCCAATTTTGGCTGCTT+CGG - Chr8:61769366-61769385 MsG0880045614.01.T01:intron 40.0%
!!! TTTTGGGTTTTTGAAGGTGG+AGG + Chr8:61768308-61768327 None:intergenic 40.0%
AACATGCGCAAGGGCAAAAT+TGG - Chr8:61768658-61768677 MsG0880045614.01.T01:CDS 45.0%
AAGATCAAAGGGGGTTTAGC+AGG - Chr8:61769325-61769344 MsG0880045614.01.T01:intron 45.0%
ACACACCCAACACAGTGAAA+TGG - Chr8:61767433-61767452 MsG0880045614.01.T01:CDS 45.0%
AGTCCATGTCAATGCTTCGT+AGG - Chr8:61770237-61770256 MsG0880045614.01.T01:intron 45.0%
ATAAGCATGTGGGGTGGAAA+AGG + Chr8:61767338-61767357 None:intergenic 45.0%
ATGATCAAACGCGATCGATG+TGG + Chr8:61770103-61770122 None:intergenic 45.0%
ATTCACGGCTACAATTGTCC+TGG - Chr8:61770009-61770028 MsG0880045614.01.T01:intron 45.0%
ATTGGATCAAGACAACGCTG+CGG + Chr8:61769355-61769374 None:intergenic 45.0%
CAGTTGTGATGCAATTGTGG+AGG + Chr8:61769931-61769950 None:intergenic 45.0%
CATGGATGCTTTGCCAATTC+AGG - Chr8:61767641-61767660 MsG0880045614.01.T01:intron 45.0%
GAAAATGGTGGTTGGAGTTC+GGG + Chr8:61768281-61768300 None:intergenic 45.0%
GAAGGTGGAGGGAAAGAAAA+TGG + Chr8:61768296-61768315 None:intergenic 45.0%
GAGGGAAAGAAAATGGTGGT+TGG + Chr8:61768289-61768308 None:intergenic 45.0%
GATGAAGCTGTTCTTCCCAT+TGG - Chr8:61770639-61770658 MsG0880045614.01.T01:five_prime_UTR 45.0%
GCGTTAGTTAACATGCGCAA+GGG - Chr8:61768649-61768668 MsG0880045614.01.T01:CDS 45.0%
GGTAAATTTGGCATGACAGC+AGG + Chr8:61768168-61768187 None:intergenic 45.0%
TGCGGTCTGCAATTGCATTT+GGG - Chr8:61768770-61768789 MsG0880045614.01.T01:intron 45.0%
TGCGTTAGTTAACATGCGCA+AGG - Chr8:61768648-61768667 MsG0880045614.01.T01:CDS 45.0%
TGTTGCTCTTAGAGCTGACA+TGG - Chr8:61767623-61767642 MsG0880045614.01.T01:intron 45.0%
TTAGCGTCACTGTATCCCTT+AGG - Chr8:61769247-61769266 MsG0880045614.01.T01:intron 45.0%
TTGCGGTCTGCAATTGCATT+TGG - Chr8:61768769-61768788 MsG0880045614.01.T01:intron 45.0%
TTGTCGTGATGTAGTTGTGG+AGG + Chr8:61769801-61769820 None:intergenic 45.0%
! CACTCTCATGTTTGGGATCA+CGG - Chr8:61767694-61767713 MsG0880045614.01.T01:intron 45.0%
! TGCAATTGCATTTGGGGTCT+TGG - Chr8:61768777-61768796 MsG0880045614.01.T01:intron 45.0%
! TGTTGTTTACCAATGGCCTG+TGG - Chr8:61767554-61767573 MsG0880045614.01.T01:intron 45.0%
!! ACAACACCAGTTTTAGCCAC+AGG + Chr8:61767573-61767592 None:intergenic 45.0%
!! CAACCTACGAAGCATTGACA+TGG + Chr8:61770243-61770262 None:intergenic 45.0%
!! GCTCTAAGAGCAACAAAAGG+AGG + Chr8:61767618-61767637 None:intergenic 45.0%
!!! CGTCCAGGTGAATTTTTAGC+AGG - Chr8:61769283-61769302 MsG0880045614.01.T01:intron 45.0%
!! TTAAAAAAAAAAAAAAAAAC+TGG - Chr8:61767873-61767892 MsG0880045614.01.T01:intron 5.0%
AGGCGTGAGCTTGACAAGTT+AGG - Chr8:61767531-61767550 MsG0880045614.01.T01:CDS 50.0%
CAACAGTACCCTGCATATGG+AGG + Chr8:61769129-61769148 None:intergenic 50.0%
CAATGGCCTGTGGCTAAAAC+TGG - Chr8:61767564-61767583 MsG0880045614.01.T01:intron 50.0%
CAGTCCTGATGCAGTTGTGA+AGG + Chr8:61770071-61770090 None:intergenic 50.0%
GATGCCGAAGCAGCCAAAAT+TGG + Chr8:61769373-61769392 None:intergenic 50.0%
GCAAAATTGGCATGGCTGCA+GGG - Chr8:61768671-61768690 MsG0880045614.01.T01:CDS 50.0%
GCACAACAGTACCCTGCATA+TGG + Chr8:61769132-61769151 None:intergenic 50.0%
GCATGTATTGCAGCGCCAAT+GGG + Chr8:61770657-61770676 None:intergenic 50.0%
GCGGTCTGCAATTGCATTTG+GGG - Chr8:61768771-61768790 MsG0880045614.01.T01:intron 50.0%
GGTGGAGGGAAAGAAAATGG+TGG + Chr8:61768293-61768312 None:intergenic 50.0%
TCTTCACTCCCTGTGACGAT+TGG + Chr8:61770513-61770532 None:intergenic 50.0%
TGCATGTATTGCAGCGCCAA+TGG + Chr8:61770658-61770677 None:intergenic 50.0%
TGCGCAAGTTAACTCACGCT+GGG - Chr8:61768190-61768209 MsG0880045614.01.T01:intron 50.0%
TGGAAGATAAGCATGTGGGG+TGG + Chr8:61767344-61767363 None:intergenic 50.0%
TTCCAACCAGCCGAGGAAAA+AGG - Chr8:61769157-61769176 MsG0880045614.01.T01:intron 50.0%
TTGCGCAAGTTAACTCACGC+TGG - Chr8:61768189-61768208 MsG0880045614.01.T01:intron 50.0%
! CTCATGTTTGGGATCACGGT+GGG - Chr8:61767698-61767717 MsG0880045614.01.T01:intron 50.0%
! GGAAAAAGGCACAGGTGCAA+AGG - Chr8:61769171-61769190 MsG0880045614.01.T01:intron 50.0%
! TCTCATGTTTGGGATCACGG+TGG - Chr8:61767697-61767716 MsG0880045614.01.T01:intron 50.0%
! TGCACCTGTGCCTTTTTCCT+CGG + Chr8:61769170-61769189 None:intergenic 50.0%
!! AGCGTGAGTTAACTTGCGCA+AGG + Chr8:61768190-61768209 None:intergenic 50.0%
!! CAGTTTTAGCCACAGGCCAT+TGG + Chr8:61767566-61767585 None:intergenic 50.0%
!! GCGTGAGTTAACTTGCGCAA+GGG + Chr8:61768189-61768208 None:intergenic 50.0%
!! GCTTATTTTCCAACCAGCCG+AGG - Chr8:61769150-61769169 MsG0880045614.01.T01:intron 50.0%
ACGCGACGCAACAACACCTA+AGG + Chr8:61769266-61769285 None:intergenic 55.0%
AGGGTGCGAGCAAAACATGC+TGG - Chr8:61768690-61768709 MsG0880045614.01.T01:CDS 55.0%
AGGGTGCGAGCAAAATGTGC+TGG + Chr8:61768148-61768167 None:intergenic 55.0%
CGCGACGCAACAACACCTAA+GGG + Chr8:61769265-61769284 None:intergenic 55.0%
GACTCCACCTGCGAGTCTAA+AGG + Chr8:61769436-61769455 None:intergenic 55.0%
GCACATGTGGCGATGCTTCT+TGG - Chr8:61768997-61769016 MsG0880045614.01.T01:intron 55.0%
GCGCAAGGGCAAAATTGGCA+TGG - Chr8:61768663-61768682 MsG0880045614.01.T01:CDS 55.0%
GGATCCTTTAGACTCGCAGG+TGG - Chr8:61769429-61769448 MsG0880045614.01.T01:intron 55.0%
GGCAAAATTGGCATGGCTGC+AGG - Chr8:61768670-61768689 MsG0880045614.01.T01:CDS 55.0%
GGGTGCGAGCAAAACATGCT+GGG - Chr8:61768691-61768710 MsG0880045614.01.T01:CDS 55.0%
GGGTGCGAGCAAAATGTGCT+GGG + Chr8:61768147-61768166 None:intergenic 55.0%
GGTGGATCCTTTAGACTCGC+AGG - Chr8:61769426-61769445 MsG0880045614.01.T01:intron 55.0%
GTGGCCTTCACAACTGCATC+AGG - Chr8:61770064-61770083 MsG0880045614.01.T01:intron 55.0%
TGCGAGCAAAACATGCTGGG+TGG - Chr8:61768694-61768713 MsG0880045614.01.T01:CDS 55.0%
TGCGAGCAAAATGTGCTGGG+TGG + Chr8:61768144-61768163 None:intergenic 55.0%
TGGTTGGAGTTCGGGCTGAT+GGG + Chr8:61768273-61768292 None:intergenic 55.0%
TTGCGGTTGCGATGTCGTTG+TGG + Chr8:61768823-61768842 None:intergenic 55.0%
TTGTGCGCACGATGCACATG+TGG - Chr8:61768984-61769003 MsG0880045614.01.T01:intron 55.0%
! TGCCTTTTTCCTCGGCTGGT+TGG + Chr8:61769162-61769181 None:intergenic 55.0%
GTGGTTGGAGTTCGGGCTGA+TGG + Chr8:61768274-61768293 None:intergenic 60.0%
! CCAGCCGAGGAAAAAGGCAC+AGG - Chr8:61769163-61769182 MsG0880045614.01.T01:intron 60.0%
! CCTGTGCCTTTTTCCTCGGC+TGG + Chr8:61769166-61769185 None:intergenic 60.0%
!! GGTGTTGTTGCGTCGCGTCC+AGG - Chr8:61769268-61769287 MsG0880045614.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 61767137 61770783 61767137 ID=MsG0880045614.01;Name=MsG0880045614.01
Chr8 mRNA 61767137 61770783 61767137 ID=MsG0880045614.01.T01;Parent=MsG0880045614.01;Name=MsG0880045614.01.T01;_AED=0.48;_eAED=0.48;_QI=145|1|1|1|1|1|5|58|440
Chr8 exon 61767137 61767572 61767137 ID=MsG0880045614.01.T01:exon:23650;Parent=MsG0880045614.01.T01
Chr8 exon 61768200 61768328 61768200 ID=MsG0880045614.01.T01:exon:23649;Parent=MsG0880045614.01.T01
Chr8 exon 61768473 61768781 61768473 ID=MsG0880045614.01.T01:exon:23648;Parent=MsG0880045614.01.T01
Chr8 exon 61768861 61768986 61768861 ID=MsG0880045614.01.T01:exon:23647;Parent=MsG0880045614.01.T01
Chr8 exon 61770258 61770783 61770258 ID=MsG0880045614.01.T01:exon:23646;Parent=MsG0880045614.01.T01
Chr8 five_prime_UTR 61770639 61770783 61770639 ID=MsG0880045614.01.T01:five_prime_utr;Parent=MsG0880045614.01.T01
Chr8 CDS 61770258 61770638 61770258 ID=MsG0880045614.01.T01:cds;Parent=MsG0880045614.01.T01
Chr8 CDS 61768861 61768986 61768861 ID=MsG0880045614.01.T01:cds;Parent=MsG0880045614.01.T01
Chr8 CDS 61768473 61768781 61768473 ID=MsG0880045614.01.T01:cds;Parent=MsG0880045614.01.T01
Chr8 CDS 61768200 61768328 61768200 ID=MsG0880045614.01.T01:cds;Parent=MsG0880045614.01.T01
Chr8 CDS 61767195 61767572 61767195 ID=MsG0880045614.01.T01:cds;Parent=MsG0880045614.01.T01
Chr8 three_prime_UTR 61767137 61767194 61767137 ID=MsG0880045614.01.T01:three_prime_utr;Parent=MsG0880045614.01.T01
Gene Sequence

>MsG0880045614.01.T01

ATGATGACTCCAAACAATCATCCAAGACTTGCTCTGTTACTCATCTTCTTGTTCCTTTTCCACCCCACATGCTTATCTTCCACTACTTATCAAACAAAAGAATGTTCAAACCAAACTTCCTCACTGAAAAGTGAGATTATTGAGCTTGCTAACACACCCAACACAGTGAAATGGATGAAGAACATCAGAAGAGAAATTCATGAGTACCCTGAACTTGCATATGAAGAATTCAAGACAAGTTCTGTCATAAGGCGTGAGCTTGACAAGTTAGGTGTTGTTTACCAATGGCCTGTGGCTAAAACTGGTGTTGTTGCTAAAATTGGTTCTGGATTTCCTCCTTTTGTTGCTCTTAGAGCTGACATGGATGCTTTGCCAATTCAGGAATTGGTTGATTGGGATCATAAAAGCAAAGTAGACGGAAAAATGCATGCTTGTGCGCACGATGCACATGTGGCGATGCTTCTTGGTGCTGCAAAGATACTGCAAGAAATGAAAGACAAATTAAAGGGTACTGTTGTGCTTATTTTCCAACCAGCCGAGGAAAAAGGCACAGGTGCAAAGGACATGATCCAAGAAAATGTGCTTGAAGATGTTGAGGCTATTTTCGGATTGCATTTAGCGTCACTGTATCCCTTAGGTGTTGTTGCGTCGCGTCCAGGTGAATTTTTAGCAGGATATGGAAGCTTCAAAGCGAAGATCAAAGGGGGTTTAGCAGGAACTCCGCAGCGTTGTCTTGATCCAATTTTGGCTGCTTCGGCATCCGTGATTAGCTTGCAAAGCATTATTTCAAGGGAGGTGGATCCTTTAGACTCGCAGGTGCTTTCTGTGGCAATGATCAATTCAGAGTCTGGTCACGAACTCACCCCTGATTCTGTAACATTTGGAGGTACTTATAGAGCATTCAGCAAAAAGAGCTTCAATACACTAAGGAATAGGATAGAAGAGGTTATCAAAGGGCAAGCAGAAGTATATAGATGCTCAGCAGAGCTTGAATTCTTTGGTGAAGAACATTCAACAATTCCTCCTACTACAAATGATGAGAGAATCTTCCAACTAGCAAGGAAAGTATCAAGTATGATTGTTGGAGAAGAGAACATAAAATTATCACCAATCGTCACAGGGAGTGAAGATTTTGCATTTTACTTGGAAAAAGTGCCTGGCTCATTTTTCTTTTTAGGTATAAAAAATGAGAAGAGTGGATCCATATATTCAGCTCATAGTCCTCACTTTTTCATTGATGAAGCTGTTCTTCCCATTGGCGCTGCAATACATGCAGCATTTGCTCTATCATATTATTCTTATTCAACAAATTCTTATCTTTAA
Protein sequence

>MsG0880045614.01.T01

MMTPNNHPRLALLLIFLFLFHPTCLSSTTYQTKECSNQTSSLKSEIIELANTPNTVKWMKNIRREIHEYPELAYEEFKTSSVIRRELDKLGVVYQWPVAKTGVVAKIGSGFPPFVALRADMDALPIQELVDWDHKSKVDGKMHACAHDAHVAMLLGAAKILQEMKDKLKGTVVLIFQPAEEKGTGAKDMIQENVLEDVEAIFGLHLASLYPLGVVASRPGEFLAGYGSFKAKIKGGLAGTPQRCLDPILAASASVISLQSIISREVDPLDSQVLSVAMINSESGHELTPDSVTFGGTYRAFSKKSFNTLRNRIEEVIKGQAEVYRCSAELEFFGEEHSTIPPTTNDERIFQLARKVSSMIVGEENIKLSPIVTGSEDFAFYLEKVPGSFFFLGIKNEKSGSIYSAHSPHFFIDEAVLPIGAAIHAAFALSYYSYSTNSYL*