Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046359.01.T01 | XP_003608955.1 | 96.791 | 187 | 6 | 0 | 1 | 187 | 1 | 187 | 1.17E-130 | 374 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048618.01 | MsG0880046359.01 | 0.801636 | 8.202398e-49 | 3.936660e-46 |
| MsG0880044145.01 | MsG0880046359.01 | 0.805614 | 1.226004e-49 | 6.515573e-47 |
| MsG0880042276.01 | MsG0880046359.01 | 0.800716 | 1.264954e-48 | 5.931413e-46 |
| MsG0880043407.01 | MsG0880046359.01 | 0.823414 | 1.403006e-53 | 1.207100e-50 |
| MsG0280010560.01 | MsG0880046359.01 | 0.819485 | 1.133350e-52 | 8.733076e-50 |
| MsG0380013176.01 | MsG0880046359.01 | 0.803288 | 3.744133e-49 | 1.874236e-46 |
| MsG0380014537.01 | MsG0880046359.01 | 0.832038 | 1.186947e-55 | 1.311162e-52 |
| MsG0380016566.01 | MsG0880046359.01 | 0.811001 | 8.709132e-51 | 5.329827e-48 |
| MsG0280009383.01 | MsG0880046359.01 | 0.804227 | 2.389668e-49 | 1.225414e-46 |
| MsG0280009598.01 | MsG0880046359.01 | 0.801091 | 1.060266e-48 | 5.019685e-46 |
| MsG0680035844.01 | MsG0880046359.01 | 0.838063 | 3.592559e-57 | 4.757047e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046359.01.T01 | MTR_4g106790 | 96.791 | 187 | 6 | 0 | 1 | 187 | 1 | 187 | 1.41e-134 | 374 |
| MsG0880046359.01.T01 | MTR_4g106790 | 95.946 | 148 | 6 | 0 | 1 | 148 | 1 | 148 | 4.18e-104 | 296 |
| MsG0880046359.01.T01 | MTR_3g078300 | 54.737 | 190 | 76 | 3 | 1 | 187 | 1 | 183 | 1.05e-62 | 192 |
| MsG0880046359.01.T01 | MTR_3g078300 | 54.967 | 151 | 58 | 3 | 1 | 148 | 1 | 144 | 1.49e-47 | 154 |
| MsG0880046359.01.T01 | MTR_5g035430 | 42.553 | 188 | 87 | 4 | 1 | 185 | 1 | 170 | 5.68e-40 | 134 |
| MsG0880046359.01.T01 | MTR_4g097220 | 38.830 | 188 | 94 | 4 | 1 | 187 | 1 | 168 | 6.50e-35 | 121 |
| MsG0880046359.01.T01 | MTR_8g063200 | 37.500 | 184 | 101 | 3 | 1 | 184 | 1 | 170 | 3.49e-31 | 111 |
| MsG0880046359.01.T01 | MTR_4g097220 | 42.857 | 133 | 57 | 2 | 1 | 133 | 1 | 114 | 2.09e-29 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046359.01.T01 | AT5G62900 | 47.059 | 187 | 73 | 4 | 1 | 187 | 1 | 161 | 4.33e-48 | 154 |
| MsG0880046359.01.T01 | AT5G50090 | 44.681 | 188 | 73 | 4 | 1 | 187 | 1 | 158 | 5.69e-42 | 139 |
| MsG0880046359.01.T01 | AT5G50090 | 46.524 | 187 | 65 | 5 | 1 | 187 | 1 | 152 | 2.74e-41 | 137 |
| MsG0880046359.01.T01 | AT1G60010 | 41.176 | 187 | 96 | 4 | 1 | 187 | 1 | 173 | 8.80e-41 | 136 |
| MsG0880046359.01.T01 | AT5G50090 | 53.782 | 119 | 42 | 1 | 1 | 119 | 1 | 106 | 1.05e-35 | 122 |
| MsG0880046359.01.T01 | AT1G10530 | 36.508 | 189 | 95 | 4 | 1 | 187 | 1 | 166 | 8.01e-34 | 118 |
| MsG0880046359.01.T01 | AT5G67620 | 32.353 | 204 | 98 | 7 | 1 | 187 | 1 | 181 | 7.82e-24 | 93.2 |
Find 36 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATCTCTTATATTTCT+AGG | 0.170393 | 8:-72394644 | None:intergenic |
| GAGGAAGGATATAAATAATA+AGG | 0.287758 | 8:-72393342 | None:intergenic |
| AACCTTTCTTCTTTGCCATT+TGG | 0.302263 | 8:-72393443 | None:intergenic |
| ACAAAAGTCAAATCAGATTA+AGG | 0.303462 | 8:+72393889 | MsG0880046359.01.T01:CDS |
| TAATATCTGAATACATTAAA+TGG | 0.306309 | 8:-72394747 | None:intergenic |
| AGATTTACCTTGTTATCTTC+AGG | 0.363890 | 8:-72393947 | None:intergenic |
| TTAATGATAAAACAGCTAAT+AGG | 0.376595 | 8:+72393918 | MsG0880046359.01.T01:CDS |
| ATCACTTCACTAGCAGTAAC+TGG | 0.406024 | 8:-72393473 | None:intergenic |
| AGAAGAGCAACATAGTGATC+AGG | 0.427746 | 8:-72393506 | None:intergenic |
| CAGAGGTTACAAAGGGTATG+TGG | 0.456771 | 8:+72393819 | MsG0880046359.01.T01:intron |
| TTCAGTGATGCTTTGTAAAG+AGG | 0.477664 | 8:-72394507 | None:intergenic |
| GCAAAGAAGAAAGGTTGTAT+TGG | 0.478560 | 8:+72393450 | MsG0880046359.01.T01:CDS |
| GAGGCTTCAAGTTGCTGAAA+GGG | 0.491174 | 8:-72394671 | None:intergenic |
| AGGACAAAAGCAACAACAAA+TGG | 0.492369 | 8:+72394449 | MsG0880046359.01.T01:CDS |
| TCAGTGATGCTTTGTAAAGA+GGG | 0.514695 | 8:-72394506 | None:intergenic |
| AACCAAATGGCAAAGAAGAA+AGG | 0.522282 | 8:+72393441 | MsG0880046359.01.T01:CDS |
| CGAGGCTTCAAGTTGCTGAA+AGG | 0.524241 | 8:-72394672 | None:intergenic |
| TACTCACATGTGATGACTCA+AGG | 0.527011 | 8:+72394558 | MsG0880046359.01.T01:three_prime_UTR |
| TTGGTTTCAGAGGTTACAAA+GGG | 0.536135 | 8:+72393812 | MsG0880046359.01.T01:intron |
| GATTCAAAACTCATATCAGC+TGG | 0.538326 | 8:-72394534 | None:intergenic |
| CTTGTGATACAGCAACCAAA+TGG | 0.541988 | 8:+72393428 | MsG0880046359.01.T01:CDS |
| ACATGATTGCTTAGATCTGT+GGG | 0.557047 | 8:+72394582 | MsG0880046359.01.T01:three_prime_UTR |
| GACATGATTGCTTAGATCTG+TGG | 0.558047 | 8:+72394581 | MsG0880046359.01.T01:three_prime_UTR |
| TTTCTTCTTCAAGAAGAGGA+AGG | 0.558868 | 8:-72393357 | None:intergenic |
| GAAGAGCAACATAGTGATCA+GGG | 0.575247 | 8:-72393505 | None:intergenic |
| CCAAGCAAAAGTGTATCTGT+TGG | 0.576593 | 8:-72393647 | None:intergenic |
| TTTGGTTTCAGAGGTTACAA+AGG | 0.584060 | 8:+72393811 | MsG0880046359.01.T01:intron |
| AGAGGTTACAAAGGGTATGT+GGG | 0.585748 | 8:+72393820 | MsG0880046359.01.T01:intron |
| TATAGACTCATCTCAGCTCA+AGG | 0.593800 | 8:+72393677 | MsG0880046359.01.T01:CDS |
| GTCTGAACCTGAAGATAACA+AGG | 0.608807 | 8:+72393940 | MsG0880046359.01.T01:CDS |
| AGTCAAATCAGATTAAGGAA+AGG | 0.621653 | 8:+72393894 | MsG0880046359.01.T01:CDS |
| ACTCACATGTGATGACTCAA+GGG | 0.627657 | 8:+72394559 | MsG0880046359.01.T01:three_prime_UTR |
| AATCTGAAAGACATGAGTCA+AGG | 0.637514 | 8:+72394429 | MsG0880046359.01.T01:CDS |
| ACAAAAGCAACAACAAATGG+TGG | 0.646145 | 8:+72394452 | MsG0880046359.01.T01:CDS |
| ATAATAATAAGTCAAGAACA+TGG | 0.691937 | 8:+72394480 | MsG0880046359.01.T01:CDS |
| ATACACAATTATTATGCACG+AGG | 0.705518 | 8:-72394690 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATAATAATTTATGAT+TGG | + | Chr8:72394385-72394404 | MsG0880046359.01.T01:intron | 10.0% |
| !! | TTATTTACATTAACTAATTA+AGG | + | Chr8:72394238-72394257 | MsG0880046359.01.T01:intron | 10.0% |
| !!! | AATTAAATATAACACATTTT+AGG | + | Chr8:72394611-72394630 | MsG0880046359.01.T01:three_prime_UTR | 10.0% |
| !!! | ATTAAATATAACACATTTTA+GGG | + | Chr8:72394612-72394631 | MsG0880046359.01.T01:three_prime_UTR | 10.0% |
| !! | CAATGCATAATATATATATA+AGG | - | Chr8:72394178-72394197 | None:intergenic | 15.0% |
| !! | TAATATATATATAAGGTTTG+AGG | - | Chr8:72394171-72394190 | None:intergenic | 15.0% |
| !! | TAATATCTGAATACATTAAA+TGG | - | Chr8:72394750-72394769 | None:intergenic | 15.0% |
| !! | TATAAAGAATCAAAAGAAAA+AGG | - | Chr8:72393733-72393752 | None:intergenic | 15.0% |
| !!! | AAGAACAAAATAATCTTTTA+AGG | - | Chr8:72393767-72393786 | None:intergenic | 15.0% |
| !!! | AGAACAAAATAATCTTTTAA+GGG | - | Chr8:72393766-72393785 | None:intergenic | 15.0% |
| !! | ATAATAATAAGTCAAGAACA+TGG | + | Chr8:72394480-72394499 | MsG0880046359.01.T01:CDS | 20.0% |
| !! | TTAATGATAAAACAGCTAAT+AGG | + | Chr8:72393918-72393937 | MsG0880046359.01.T01:CDS | 20.0% |
| !! | TTATCATCTCTTATATTTCT+AGG | - | Chr8:72394647-72394666 | None:intergenic | 20.0% |
| ! | AAAAAGAAGTGAGAGATAAT+GGG | + | Chr8:72393377-72393396 | MsG0880046359.01.T01:exon | 25.0% |
| ! | ACAAAAGTCAAATCAGATTA+AGG | + | Chr8:72393889-72393908 | MsG0880046359.01.T01:CDS | 25.0% |
| ! | GAGGAAGGATATAAATAATA+AGG | - | Chr8:72393345-72393364 | None:intergenic | 25.0% |
| ! | TACATTAACTAATTAAGGTC+TGG | + | Chr8:72394243-72394262 | MsG0880046359.01.T01:intron | 25.0% |
| ! | TATAGTTACTGTTCATAAGA+AGG | - | Chr8:72394094-72394113 | None:intergenic | 25.0% |
| !! | GGTCTGGTTATAAATTTTAT+TGG | + | Chr8:72394259-72394278 | MsG0880046359.01.T01:intron | 25.0% |
| !!! | AATATAACACATTTTAGGGA+AGG | + | Chr8:72394616-72394635 | MsG0880046359.01.T01:three_prime_UTR | 25.0% |
| !!! | ATGATTGGTTCATTATTTTC+AGG | + | Chr8:72394400-72394419 | MsG0880046359.01.T01:intron | 25.0% |
| !!! | TTGAGTCTTTATGTTGTTTT+TGG | + | Chr8:72393793-72393812 | MsG0880046359.01.T01:intron | 25.0% |
| AAGAACATGAATATCTTCAG+TGG | + | Chr8:72394027-72394046 | MsG0880046359.01.T01:intron | 30.0% | |
| AGATTCAATCAACATGGATT+TGG | - | Chr8:72394005-72394024 | None:intergenic | 30.0% | |
| AGTCAAATCAGATTAAGGAA+AGG | + | Chr8:72393894-72393913 | MsG0880046359.01.T01:CDS | 30.0% | |
| ATACACAATTATTATGCACG+AGG | - | Chr8:72394693-72394712 | None:intergenic | 30.0% | |
| GAAAAAGAAGTGAGAGATAA+TGG | + | Chr8:72393376-72393395 | MsG0880046359.01.T01:exon | 30.0% | |
| ! | AGATTTACCTTGTTATCTTC+AGG | - | Chr8:72393950-72393969 | None:intergenic | 30.0% |
| ! | TCTTTTTCTTCTTCAAGAAG+AGG | - | Chr8:72393364-72393383 | None:intergenic | 30.0% |
| !! | TAAGAGAGAGATTTGTTGAA+TGG | + | Chr8:72394361-72394380 | MsG0880046359.01.T01:intron | 30.0% |
| !!! | TATGTTGTTTTTGGTTTCAG+AGG | + | Chr8:72393802-72393821 | MsG0880046359.01.T01:intron | 30.0% |
| !!! | TTTTGAAGTGCATAATGTTG+TGG | - | Chr8:72393537-72393556 | None:intergenic | 30.0% |
| AACCAAATGGCAAAGAAGAA+AGG | + | Chr8:72393441-72393460 | MsG0880046359.01.T01:CDS | 35.0% | |
| AACCTTTCTTCTTTGCCATT+TGG | - | Chr8:72393446-72393465 | None:intergenic | 35.0% | |
| AATCTGAAAGACATGAGTCA+AGG | + | Chr8:72394429-72394448 | MsG0880046359.01.T01:CDS | 35.0% | |
| ACAAAAGCAACAACAAATGG+TGG | + | Chr8:72394452-72394471 | MsG0880046359.01.T01:CDS | 35.0% | |
| ACATGATTGCTTAGATCTGT+GGG | + | Chr8:72394582-72394601 | MsG0880046359.01.T01:three_prime_UTR | 35.0% | |
| AGGACAAAAGCAACAACAAA+TGG | + | Chr8:72394449-72394468 | MsG0880046359.01.T01:CDS | 35.0% | |
| GATTCAAAACTCATATCAGC+TGG | - | Chr8:72394537-72394556 | None:intergenic | 35.0% | |
| GCAAAGAAGAAAGGTTGTAT+TGG | + | Chr8:72393450-72393469 | MsG0880046359.01.T01:CDS | 35.0% | |
| TACTGTTCATAAGAAGGAGT+TGG | - | Chr8:72394088-72394107 | None:intergenic | 35.0% | |
| TCAGTGATGCTTTGTAAAGA+GGG | - | Chr8:72394509-72394528 | None:intergenic | 35.0% | |
| TCTTGCAGATTCAATCAACA+TGG | - | Chr8:72394011-72394030 | None:intergenic | 35.0% | |
| TTCAGTGATGCTTTGTAAAG+AGG | - | Chr8:72394510-72394529 | None:intergenic | 35.0% | |
| TTGGTTTCAGAGGTTACAAA+GGG | + | Chr8:72393812-72393831 | MsG0880046359.01.T01:intron | 35.0% | |
| TTTCTTCTTCAAGAAGAGGA+AGG | - | Chr8:72393360-72393379 | None:intergenic | 35.0% | |
| ! | TTTGGTTTCAGAGGTTACAA+AGG | + | Chr8:72393811-72393830 | MsG0880046359.01.T01:intron | 35.0% |
| !!! | TTTGACTTTTGTGCTGATTC+TGG | - | Chr8:72393881-72393900 | None:intergenic | 35.0% |
| ACTCACATGTGATGACTCAA+GGG | + | Chr8:72394559-72394578 | MsG0880046359.01.T01:three_prime_UTR | 40.0% | |
| AGAAGAGCAACATAGTGATC+AGG | - | Chr8:72393509-72393528 | None:intergenic | 40.0% | |
| ATCACTTCACTAGCAGTAAC+TGG | - | Chr8:72393476-72393495 | None:intergenic | 40.0% | |
| CCTTGCTACTACAAAGTCAA+TGG | - | Chr8:72394311-72394330 | None:intergenic | 40.0% | |
| CTTGTGATACAGCAACCAAA+TGG | + | Chr8:72393428-72393447 | MsG0880046359.01.T01:CDS | 40.0% | |
| GAAGAGCAACATAGTGATCA+GGG | - | Chr8:72393508-72393527 | None:intergenic | 40.0% | |
| GACATGATTGCTTAGATCTG+TGG | + | Chr8:72394581-72394600 | MsG0880046359.01.T01:three_prime_UTR | 40.0% | |
| GTCTGAACCTGAAGATAACA+AGG | + | Chr8:72393940-72393959 | MsG0880046359.01.T01:CDS | 40.0% | |
| TACTCACATGTGATGACTCA+AGG | + | Chr8:72394558-72394577 | MsG0880046359.01.T01:three_prime_UTR | 40.0% | |
| TATAGACTCATCTCAGCTCA+AGG | + | Chr8:72393677-72393696 | MsG0880046359.01.T01:CDS | 40.0% | |
| ! | AGAGGTTACAAAGGGTATGT+GGG | + | Chr8:72393820-72393839 | MsG0880046359.01.T01:intron | 40.0% |
| ! | CCAACAGATACACTTTTGCT+TGG | + | Chr8:72393647-72393666 | MsG0880046359.01.T01:CDS | 40.0% |
| ! | CCAAGCAAAAGTGTATCTGT+TGG | - | Chr8:72393650-72393669 | None:intergenic | 40.0% |
| !! | CCATTGACTTTGTAGTAGCA+AGG | + | Chr8:72394308-72394327 | MsG0880046359.01.T01:intron | 40.0% |
| ! | CAGAGGTTACAAAGGGTATG+TGG | + | Chr8:72393819-72393838 | MsG0880046359.01.T01:intron | 45.0% |
| !! | GAGGCTTCAAGTTGCTGAAA+GGG | - | Chr8:72394674-72394693 | None:intergenic | 45.0% |
| !! | CGAGGCTTCAAGTTGCTGAA+AGG | - | Chr8:72394675-72394694 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 72393340 | 72394803 | 72393340 | ID=MsG0880046359.01;Name=MsG0880046359.01 |
| Chr8 | mRNA | 72393340 | 72394803 | 72393340 | ID=MsG0880046359.01.T01;Parent=MsG0880046359.01;Name=MsG0880046359.01.T01;_AED=0.50;_eAED=0.50;_QI=55|1|1|1|1|1|3|262|187 |
| Chr8 | exon | 72393340 | 72393698 | 72393340 | ID=MsG0880046359.01.T01:exon:7878;Parent=MsG0880046359.01.T01 |
| Chr8 | exon | 72393822 | 72393961 | 72393822 | ID=MsG0880046359.01.T01:exon:7879;Parent=MsG0880046359.01.T01 |
| Chr8 | exon | 72394422 | 72394803 | 72394422 | ID=MsG0880046359.01.T01:exon:7880;Parent=MsG0880046359.01.T01 |
| Chr8 | five_prime_UTR | 72393340 | 72393394 | 72393340 | ID=MsG0880046359.01.T01:five_prime_utr;Parent=MsG0880046359.01.T01 |
| Chr8 | CDS | 72393395 | 72393698 | 72393395 | ID=MsG0880046359.01.T01:cds;Parent=MsG0880046359.01.T01 |
| Chr8 | CDS | 72393822 | 72393961 | 72393822 | ID=MsG0880046359.01.T01:cds;Parent=MsG0880046359.01.T01 |
| Chr8 | CDS | 72394422 | 72394541 | 72394422 | ID=MsG0880046359.01.T01:cds;Parent=MsG0880046359.01.T01 |
| Chr8 | three_prime_UTR | 72394542 | 72394803 | 72394542 | ID=MsG0880046359.01.T01:three_prime_utr;Parent=MsG0880046359.01.T01 |
| Gene Sequence |
| Protein sequence |
