AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016566.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016566.01.T01 MTR_3g092020 92.982 171 3 1 1 171 1 162 8.86e-115 322
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTAAGGTTTCCATTT+TGG 0.114718 3:-85237430 None:intergenic
CTCCCCATCTCTCCTTAAAA+AGG 0.245866 3:-85238590 None:intergenic
CACTGTGAACTTTCACTATT+GGG 0.252264 3:-85239399 None:intergenic
CCACTGTGAACTTTCACTAT+TGG 0.288552 3:-85239400 None:intergenic
GTTATATCTTACTACAATAT+TGG 0.321352 3:+85239526 MsG0380016566.01.T01:three_prime_UTR
CTATAATCTAATCCCCAAAA+TGG 0.329374 3:+85237416 MsG0380016566.01.T01:exon
AAAACAATTTGTCAAATTCA+AGG 0.363779 3:-85238514 None:intergenic
AAAGGTAGCAACAGTTTCCT+TGG 0.390750 3:-85237335 None:intergenic
CAAAATGGAAACCTTACCTT+TGG 0.392712 3:+85237431 MsG0380016566.01.T01:exon
CTCCTCTTGGTTCAAAATCA+TGG 0.393681 3:+85238634 MsG0380016566.01.T01:CDS
GAGATCATGTCGAGGAATCT+TGG 0.402090 3:+85239288 MsG0380016566.01.T01:CDS
AATATATAATTTAATTATCA+AGG 0.424029 3:-85239582 None:intergenic
TGGCCTCGGACAATGCTCTA+TGG 0.432233 3:+85238562 MsG0380016566.01.T01:CDS
TTATATCACACGGCTGCGTT+TGG 0.432650 3:-85239359 None:intergenic
CATACAGATTTCCAAAGGTA+AGG 0.450513 3:-85237442 None:intergenic
ACTTTCACTATTGGGTTGAA+TGG 0.462372 3:-85239391 None:intergenic
GGTGAGGTACAACGATATCT+AGG 0.477811 3:+85239181 MsG0380016566.01.T01:CDS
AGTTCAGAAACAGACTTCAC+TGG 0.488792 3:+85239244 MsG0380016566.01.T01:CDS
GAAGTGGAAGCTCATGGCCT+CGG 0.508465 3:+85238548 MsG0380016566.01.T01:CDS
GGTTTGAAGATTGTTAGAGA+AGG 0.530829 3:+85239133 MsG0380016566.01.T01:CDS
TTGTTGAGAAACAGCAAGAT+GGG 0.537051 3:-85237490 None:intergenic
ATCATGGAAAGAAGTGTATG+AGG 0.545115 3:+85238650 MsG0380016566.01.T01:CDS
CATCTTGCTGTTTCTCAACA+AGG 0.545411 3:+85237492 MsG0380016566.01.T01:CDS
CAAGATCGATGCGATCGAGT+TGG 0.561970 3:+85238675 MsG0380016566.01.T01:CDS
ATTGGGTTGAATGGTAGAGA+TGG 0.590103 3:-85239382 None:intergenic
CTGTAGGAAGTGGAAGCTCA+TGG 0.592096 3:+85238542 MsG0380016566.01.T01:CDS
CTTGTTGAGAAACAGCAAGA+TGG 0.595023 3:-85237491 None:intergenic
ATCTTCATACAGATTTCCAA+AGG 0.614774 3:-85237447 None:intergenic
GAGAAACAGCAAGATGGGTG+TGG 0.636024 3:-85237485 None:intergenic
GATATCGTTGTACCTCACCT+TGG 0.640965 3:-85239177 None:intergenic
CTATCTTGTTCACCAAGGTG+AGG 0.666547 3:+85239165 MsG0380016566.01.T01:CDS
GTAGAGATGGTTATATCACA+CGG 0.677630 3:-85239369 None:intergenic
TGACCATAGAGCATTGTCCG+AGG 0.683112 3:-85238565 None:intergenic
GCTGAAGAGAGATCATGTCG+AGG 0.706201 3:+85239280 MsG0380016566.01.T01:CDS
GACTACTATCTTGTTCACCA+AGG 0.711703 3:+85239160 MsG0380016566.01.T01:CDS
CCAATAGTGAAAGTTCACAG+TGG 0.758241 3:+85239400 MsG0380016566.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAATTAATAATAATTT+TGG + Chr3:85238863-85238882 MsG0380016566.01.T01:intron 0.0%
!!! AAAAAATTAACAAATTGTTT+TGG - Chr3:85238329-85238348 None:intergenic 10.0%
!!! TTTTAGAAGAAAAAAAATAT+GGG - Chr3:85238892-85238911 None:intergenic 10.0%
!! AATTGAATCAATCATAATAT+TGG + Chr3:85238228-85238247 MsG0380016566.01.T01:intron 15.0%
!! AATTGTATAACAAAATTTGT+TGG - Chr3:85237914-85237933 None:intergenic 15.0%
!! TTTACTGTAATGATATAAAA+AGG - Chr3:85238210-85238229 None:intergenic 15.0%
!!! AACAATAAAGATTGAATTTT+AGG - Chr3:85238122-85238141 None:intergenic 15.0%
!!! ACAAAATTCTTTTTTTCATT+GGG + Chr3:85239311-85239330 MsG0380016566.01.T01:CDS 15.0%
!!! ATAATTTGTTATGTTGAATA+TGG - Chr3:85238997-85239016 None:intergenic 15.0%
!!! GTTTTAGAAGAAAAAAAATA+TGG - Chr3:85238893-85238912 None:intergenic 15.0%
!!! TTATATATTCAGTTATTTTG+AGG + Chr3:85239596-85239615 MsG0380016566.01.T01:three_prime_UTR 15.0%
!!! TTTTAATTCTTCACAAAATA+GGG + Chr3:85239111-85239130 MsG0380016566.01.T01:intron 15.0%
!!! TTTTTAATTCTTCACAAAAT+AGG + Chr3:85239110-85239129 MsG0380016566.01.T01:intron 15.0%
!! AAAACAATTTGTCAAATTCA+AGG - Chr3:85238517-85238536 None:intergenic 20.0%
!! ATCAATCATAATATTGGATT+AGG + Chr3:85238234-85238253 MsG0380016566.01.T01:intron 20.0%
!! GTTATATCTTACTACAATAT+TGG + Chr3:85239526-85239545 MsG0380016566.01.T01:three_prime_UTR 20.0%
!! TATGTATGTATAAAAAACTG+TGG + Chr3:85239552-85239571 MsG0380016566.01.T01:three_prime_UTR 20.0%
!! TTATTGCTTCAAATTAAACT+AGG - Chr3:85237958-85237977 None:intergenic 20.0%
!! TTTAATTCTTCACAAAATAG+GGG + Chr3:85239112-85239131 MsG0380016566.01.T01:intron 20.0%
!!! ATTTGAAATTCATAAGTCAA+CGG - Chr3:85237614-85237633 None:intergenic 20.0%
!!! CAAAATTCTTTTTTTCATTG+GGG + Chr3:85239312-85239331 MsG0380016566.01.T01:CDS 20.0%
!!! CTGAGTTTATTACATTATTT+TGG + Chr3:85238486-85238505 MsG0380016566.01.T01:intron 20.0%
!!! GACAAAATTCTTTTTTTCAT+TGG + Chr3:85239310-85239329 MsG0380016566.01.T01:CDS 20.0%
!!! TCAATCTTTATTGTTTTGAT+CGG + Chr3:85238127-85238146 MsG0380016566.01.T01:intron 20.0%
!!! TTGTCAAATATCTTTTTAGA+GGG - Chr3:85238436-85238455 None:intergenic 20.0%
! AAAATCTCCTTCAATAATCA+TGG - Chr3:85238783-85238802 None:intergenic 25.0%
! AACCTATGTGTAACATTAAA+AGG - Chr3:85238360-85238379 None:intergenic 25.0%
! AATAACAATATTTCAACCGA+TGG - Chr3:85237649-85237668 None:intergenic 25.0%
! ATCTAGGTTCAAGAAAAAAT+CGG + Chr3:85239197-85239216 MsG0380016566.01.T01:CDS 25.0%
! CAAGAATATAAAAATGACGA+TGG - Chr3:85237548-85237567 None:intergenic 25.0%
! GTTTCAAGAAAGAGAAAAAA+AGG - Chr3:85237356-85237375 None:intergenic 25.0%
! TCTTTATCATAAAACAGTGT+TGG + Chr3:85237768-85237787 MsG0380016566.01.T01:intron 25.0%
! TGTTGGATATCATATGATAT+AGG - Chr3:85237897-85237916 None:intergenic 25.0%
! TTAAATGCCATGATTATTGA+AGG + Chr3:85238773-85238792 MsG0380016566.01.T01:intron 25.0%
! TTATTAGATCTGAATCTGTA+TGG + Chr3:85237664-85237683 MsG0380016566.01.T01:intron 25.0%
!! ACTGTGATTTTATGATACAT+TGG + Chr3:85238163-85238182 MsG0380016566.01.T01:intron 25.0%
!! CTTGTCAAATATCTTTTTAG+AGG - Chr3:85238437-85238456 None:intergenic 25.0%
!! TTCCTTTTAATGTTACACAT+AGG + Chr3:85238355-85238374 MsG0380016566.01.T01:intron 25.0%
AAATATGAATCAAGTCCATG+TGG - Chr3:85238086-85238105 None:intergenic 30.0%
ATAATTTGAGAAAGACAGAC+AGG - Chr3:85237710-85237729 None:intergenic 30.0%
ATCTTCATACAGATTTCCAA+AGG - Chr3:85237450-85237469 None:intergenic 30.0%
CTATAATCTAATCCCCAAAA+TGG + Chr3:85237416-85237435 MsG0380016566.01.T01:exon 30.0%
TTATTGAACTCTGACACAAA+GGG + Chr3:85239080-85239099 MsG0380016566.01.T01:intron 30.0%
TTTCTGCACACTTATAACAT+TGG + Chr3:85238927-85238946 MsG0380016566.01.T01:intron 30.0%
! AAAGGTAAGGTTTCCATTTT+GGG - Chr3:85237432-85237451 None:intergenic 30.0%
! TCTATGGTCAAACCTTTTTA+AGG + Chr3:85238578-85238597 MsG0380016566.01.T01:CDS 30.0%
! TTATCATAAAACAGTGTTGG+TGG + Chr3:85237771-85237790 MsG0380016566.01.T01:intron 30.0%
!! AAACCTTTTTAAGGAGAGAT+GGG + Chr3:85238587-85238606 MsG0380016566.01.T01:CDS 30.0%
!! CAAATTGTTTTACAGTCTGT+AGG + Chr3:85238526-85238545 MsG0380016566.01.T01:CDS 30.0%
!!! TCTTTTTTTCATTGGGGATT+TGG + Chr3:85239318-85239337 MsG0380016566.01.T01:CDS 30.0%
!!! TTTTGTTTTCTGCGAAGAAA+TGG + Chr3:85237837-85237856 MsG0380016566.01.T01:intron 30.0%
AAATCAGTTGCATGTTCCAT+CGG + Chr3:85237630-85237649 MsG0380016566.01.T01:intron 35.0%
ATAATTGCAAGCGTGTGATT+AGG - Chr3:85238817-85238836 None:intergenic 35.0%
ATAGGTTAAAACCGACGATT+TGG + Chr3:85238373-85238392 MsG0380016566.01.T01:intron 35.0%
ATTATTCTTGTCCCTTGAGA+TGG + Chr3:85238832-85238851 MsG0380016566.01.T01:intron 35.0%
ATTTGGCAACTGAGTGTTAA+CGG + Chr3:85238397-85238416 MsG0380016566.01.T01:intron 35.0%
CAAAATGGAAACCTTACCTT+TGG + Chr3:85237431-85237450 MsG0380016566.01.T01:exon 35.0%
CACACTTATAACATTGGTGT+TGG + Chr3:85238933-85238952 MsG0380016566.01.T01:intron 35.0%
CACTGTGAACTTTCACTATT+GGG - Chr3:85239402-85239421 None:intergenic 35.0%
CATACAGATTTCCAAAGGTA+AGG - Chr3:85237445-85237464 None:intergenic 35.0%
CTAAAGCAAATCCAAGTAAG+AGG - Chr3:85238962-85238981 None:intergenic 35.0%
CTTATTGAACTCTGACACAA+AGG + Chr3:85239079-85239098 MsG0380016566.01.T01:intron 35.0%
GGATTAACAATATGTGACAG+AGG - Chr3:85237876-85237895 None:intergenic 35.0%
GGTCAAATTCCAGATCATAA+TGG - Chr3:85238055-85238074 None:intergenic 35.0%
GGTTTGAAGATTGTTAGAGA+AGG + Chr3:85239133-85239152 MsG0380016566.01.T01:CDS 35.0%
GTAGAGATGGTTATATCACA+CGG - Chr3:85239372-85239391 None:intergenic 35.0%
TTGTTGAGAAACAGCAAGAT+GGG - Chr3:85237493-85237512 None:intergenic 35.0%
TTTGACCAAAATTTGCCACA+TGG + Chr3:85238068-85238087 MsG0380016566.01.T01:intron 35.0%
! AAGGTAAGGTTTCCATTTTG+GGG - Chr3:85237431-85237450 None:intergenic 35.0%
! ATAATTTTCACGCCATCTCA+AGG - Chr3:85238847-85238866 None:intergenic 35.0%
! ATCATGGAAAGAAGTGTATG+AGG + Chr3:85238650-85238669 MsG0380016566.01.T01:CDS 35.0%
! CAAAGGTAAGGTTTCCATTT+TGG - Chr3:85237433-85237452 None:intergenic 35.0%
! CTGTGTTTCTTTGAAAAGCA+TGG - Chr3:85238021-85238040 None:intergenic 35.0%
! TAATTTTCACGCCATCTCAA+GGG - Chr3:85238846-85238865 None:intergenic 35.0%
! TTCCATGATTTTGAACCAAG+AGG - Chr3:85238639-85238658 None:intergenic 35.0%
!! AAAACCGACGATTTGGTATT+TGG + Chr3:85238380-85238399 MsG0380016566.01.T01:intron 35.0%
!! AACCTTTTTAAGGAGAGATG+GGG + Chr3:85238588-85238607 MsG0380016566.01.T01:CDS 35.0%
!! ACTTTCACTATTGGGTTGAA+TGG - Chr3:85239394-85239413 None:intergenic 35.0%
!! CAAACCTTTTTAAGGAGAGA+TGG + Chr3:85238586-85238605 MsG0380016566.01.T01:CDS 35.0%
!!! TTCCTTGGATTCTGTTTTTG+TGG - Chr3:85237323-85237342 None:intergenic 35.0%
AAAAATCGGCGACAAGTATC+TGG + Chr3:85239211-85239230 MsG0380016566.01.T01:CDS 40.0%
AAACACACCGCTATGTTTCT+TGG + Chr3:85237977-85237996 MsG0380016566.01.T01:intron 40.0%
AAAGGTAGCAACAGTTTCCT+TGG - Chr3:85237338-85237357 None:intergenic 40.0%
AACACACCGCTATGTTTCTT+GGG + Chr3:85237978-85237997 MsG0380016566.01.T01:intron 40.0%
AGTTCAGAAACAGACTTCAC+TGG + Chr3:85239244-85239263 MsG0380016566.01.T01:CDS 40.0%
CCAATAGTGAAAGTTCACAG+TGG + Chr3:85239400-85239419 MsG0380016566.01.T01:three_prime_UTR 40.0%
CCACTGTGAACTTTCACTAT+TGG - Chr3:85239403-85239422 None:intergenic 40.0%
CGTGCAACAAAAACAGTAAC+TGG - Chr3:85237383-85237402 None:intergenic 40.0%
CTCCTCTTGGTTCAAAATCA+TGG + Chr3:85238634-85238653 MsG0380016566.01.T01:CDS 40.0%
CTTGTTGAGAAACAGCAAGA+TGG - Chr3:85237494-85237513 None:intergenic 40.0%
GACCACAAAAACAGAATCCA+AGG + Chr3:85237318-85237337 MsG0380016566.01.T01:five_prime_UTR 40.0%
GACTACTATCTTGTTCACCA+AGG + Chr3:85239160-85239179 MsG0380016566.01.T01:CDS 40.0%
GCAAATCAGCAAGACATATG+CGG - Chr3:85238307-85238326 None:intergenic 40.0%
GGTTCACAGAACACAGTTAT+TGG - Chr3:85237937-85237956 None:intergenic 40.0%
GTTGCCAAATACCAAATCGT+CGG - Chr3:85238387-85238406 None:intergenic 40.0%
TTACTCTGCTGAGAGTAGTA+CGG + Chr3:85238715-85238734 MsG0380016566.01.T01:intron 40.0%
! CAAGTCCATGTGGCAAATTT+TGG - Chr3:85238076-85238095 None:intergenic 40.0%
! GTGTTGGTGGAATTGAAAAC+AGG + Chr3:85237784-85237803 MsG0380016566.01.T01:intron 40.0%
! GTTTTACAGTCTGTAGGAAG+TGG + Chr3:85238532-85238551 MsG0380016566.01.T01:CDS 40.0%
!! ATTGGGTTGAATGGTAGAGA+TGG - Chr3:85239385-85239404 None:intergenic 40.0%
!! CATCTTGCTGTTTCTCAACA+AGG + Chr3:85237492-85237511 MsG0380016566.01.T01:CDS 40.0%
CCTCAGCTTCCATTATGATC+TGG + Chr3:85238043-85238062 MsG0380016566.01.T01:intron 45.0%
CTATCTTGTTCACCAAGGTG+AGG + Chr3:85239165-85239184 MsG0380016566.01.T01:CDS 45.0%
CTCCCCATCTCTCCTTAAAA+AGG - Chr3:85238593-85238612 None:intergenic 45.0%
GAGATCATGTCGAGGAATCT+TGG + Chr3:85239288-85239307 MsG0380016566.01.T01:CDS 45.0%
GATATCGTTGTACCTCACCT+TGG - Chr3:85239180-85239199 None:intergenic 45.0%
GCATGGTGAAAGTTAGAGTC+TGG - Chr3:85238004-85238023 None:intergenic 45.0%
GGTGAGGTACAACGATATCT+AGG + Chr3:85239181-85239200 MsG0380016566.01.T01:CDS 45.0%
TTATATCACACGGCTGCGTT+TGG - Chr3:85239362-85239381 None:intergenic 45.0%
! CCAGATCATAATGGAAGCTG+AGG - Chr3:85238046-85238065 None:intergenic 45.0%
!! CTTTTTAAGGAGAGATGGGG+AGG + Chr3:85238591-85238610 MsG0380016566.01.T01:CDS 45.0%
!!! GCTGCTTTTTATGCTCCTCT+TGG + Chr3:85238621-85238640 MsG0380016566.01.T01:CDS 45.0%
!! AATATATAATTTAATTATCA+AGG - Chr3:85239585-85239604 None:intergenic 5.0%
CAAGATCGATGCGATCGAGT+TGG + Chr3:85238675-85238694 MsG0380016566.01.T01:CDS 50.0%
CTGTAGGAAGTGGAAGCTCA+TGG + Chr3:85238542-85238561 MsG0380016566.01.T01:CDS 50.0%
GAGAAACAGCAAGATGGGTG+TGG - Chr3:85237488-85237507 None:intergenic 50.0%
GCTGAAGAGAGATCATGTCG+AGG + Chr3:85239280-85239299 MsG0380016566.01.T01:CDS 50.0%
GTCTGGCCCAAGAAACATAG+CGG - Chr3:85237987-85238006 None:intergenic 50.0%
!! GGTGTTGGCTTCCTCTTACT+TGG + Chr3:85238948-85238967 MsG0380016566.01.T01:intron 50.0%
!! TGACCATAGAGCATTGTCCG+AGG - Chr3:85238568-85238587 None:intergenic 50.0%
GAAGTGGAAGCTCATGGCCT+CGG + Chr3:85238548-85238567 MsG0380016566.01.T01:CDS 55.0%
TGGCCTCGGACAATGCTCTA+TGG + Chr3:85238562-85238581 MsG0380016566.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 85237316 85239615 85237316 ID=MsG0380016566.01;Name=MsG0380016566.01
Chr3 mRNA 85237316 85239615 85237316 ID=MsG0380016566.01.T01;Parent=MsG0380016566.01;Name=MsG0380016566.01.T01;_AED=0.44;_eAED=0.45;_QI=119|0.5|0.66|1|0.5|0.33|3|234|171
Chr3 exon 85237316 85237513 85237316 ID=MsG0380016566.01.T01:exon:14479;Parent=MsG0380016566.01.T01
Chr3 exon 85238514 85238700 85238514 ID=MsG0380016566.01.T01:exon:14480;Parent=MsG0380016566.01.T01
Chr3 exon 85239132 85239615 85239132 ID=MsG0380016566.01.T01:exon:14481;Parent=MsG0380016566.01.T01
Chr3 five_prime_UTR 85237316 85237434 85237316 ID=MsG0380016566.01.T01:five_prime_utr;Parent=MsG0380016566.01.T01
Chr3 CDS 85237435 85237513 85237435 ID=MsG0380016566.01.T01:cds;Parent=MsG0380016566.01.T01
Chr3 CDS 85238514 85238700 85238514 ID=MsG0380016566.01.T01:cds;Parent=MsG0380016566.01.T01
Chr3 CDS 85239132 85239381 85239132 ID=MsG0380016566.01.T01:cds;Parent=MsG0380016566.01.T01
Chr3 three_prime_UTR 85239382 85239615 85239382 ID=MsG0380016566.01.T01:three_prime_utr;Parent=MsG0380016566.01.T01
Gene Sequence

>MsG0380016566.01.T01

ATGGAAACCTTACCTTTGGAAATCTGTATGAAGATATTCTGTTTCTTAGACCACACCCATCTTGCTGTTTCTCAACAAGCCTTGAATTTGACAAATTGTTTTACAGTCTGTAGGAAGTGGAAGCTCATGGCCTCGGACAATGCTCTATGGTCAAACCTTTTTAAGGAGAGATGGGGAGGAGATCATGCTGCTTTTTATGCTCCTCTTGGTTCAAAATCATGGAAAGAAGTGTATGAGGCGCAAGATCGATGCGATCGAGTTGGATTGGGTTTGAAGATTGTTAGAGAAGGAAGTGACTACTATCTTGTTCACCAAGGTGAGGTACAACGATATCTAGGTTCAAGAAAAAATCGGCGACAAGTATCTGGTCATACTTCTAGTTCAGAAACAGACTTCACTGGAGAAAGTTATGTAGCTGAAGAGAGATCATGTCGAGGAATCTTGGACAAAATTCTTTTTTTCATTGGGGATTTGGAAGCTGCTTCTGTTGATGCCAAACGCAGCCGTGTGATATAA

Protein sequence

>MsG0380016566.01.T01

METLPLEICMKIFCFLDHTHLAVSQQALNLTNCFTVCRKWKLMASDNALWSNLFKERWGGDHAAFYAPLGSKSWKEVYEAQDRCDRVGLGLKIVREGSDYYLVHQGEVQRYLGSRKNRRQVSGHTSSSETDFTGESYVAEERSCRGILDKILFFIGDLEAASVDAKRSRVI*