AlfalfaGEDB | MsG0880047145.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047145.01

KEGG: K02905
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF01779

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047145.01.T01	KAE9602565.1	100	61	0	0	22	82	1	61	2.44E-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047145.01.T01	Q9M7X7	83.607	61	10	0	22	82	1	61	2.49E-30	104

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047145.01.T01	A0A6A4PLY0	100.000	61	0	0	22	82	1	61	1.16e-35	124

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002547.01	MsG0880047145.01	0.827023	1.966680e-54	1.875727e-51
MsG0880045656.01	MsG0880047145.01	0.801277	9.714816e-49	4.620736e-46
MsG0180004861.01	MsG0880047145.01	0.815156	1.068209e-51	7.307653e-49
MsG0880042991.01	MsG0880047145.01	0.802272	6.069465e-49	2.960609e-46
MsG0380013085.01	MsG0880047145.01	0.805542	1.269408e-49	6.733583e-47
MsG0480022960.01	MsG0880047145.01	0.810720	1.002006e-50	6.085740e-48
MsG0480023221.01	MsG0880047145.01	0.822013	2.972541e-53	2.457880e-50
MsG0380016290.01	MsG0880047145.01	0.803089	4.117019e-49	2.050326e-46
MsG0280006993.01	MsG0880047145.01	0.817977	2.491732e-52	1.841576e-49
MsG0280007527.01	MsG0880047145.01	0.816417	5.590912e-52	3.959200e-49
MsG0280009575.01	MsG0880047145.01	0.842841	2.020682e-58	3.102500e-55

PPI

Gene1	Gene2	Type
MsG0880047145.01	MsG0380014248.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047145.01.T01	MTR_4g121877	98.361	61	1	0	22	82	1	61	1.40e-38	122
MsG0880047145.01.T01	MTR_4g121870	93.443	61	4	0	22	82	137	197	1.72e-36	121

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047145.01.T01	AT3G06700	83.607	61	10	22	82	1	61	2.54e-31	104
MsG0880047145.01.T01	AT3G06700	83.607	61	10	22	82	1	61	2.54e-31	104
MsG0880047145.01.T01	AT3G06700	83.607	61	10	22	82	1	61	2.54e-31	104
MsG0880047145.01.T01	AT3G06680	83.607	61	10	22	82	23	83	5.63e-31	104
MsG0880047145.01.T01	AT3G06680	83.607	61	10	22	82	1	61	8.77e-31	103
MsG0880047145.01.T01	AT3G06680	83.607	61	10	22	82	1	61	8.77e-31	103

Find 22 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGTGTGGCGATGCCTCTT+TGG	0.338170	8:-82624528	None:intergenic
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	8:-82624491	None:intergenic
TGTGCTTCCTTGCATACCTC+TGG	0.438138	8:-82624671	None:intergenic
ATCGCCACACTTCCACCAAA+GGG	0.487791	8:+82624538	MsG0880047145.01.T01:CDS
CTGAGTAATACATACCCCTT+TGG	0.488635	8:-82624553	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	8:+82624482	MsG0880047145.01.T01:CDS
TCTTACAAGGCTCACAAGAA+TGG	0.521704	8:+82624495	MsG0880047145.01.T01:CDS
ACAAGATAGCCTATGGGAGG+AGG	0.537841	8:+82623791	None:intergenic
CATCGCCACACTTCCACCAA+AGG	0.542258	8:+82624537	MsG0880047145.01.T01:CDS
TCACGTGAGGGAGAAGAAAG+AGG	0.543044	8:+82623817	MsG0880047145.01.T01:CDS
ACGTGATCTCCTCCTCCCAT+AGG	0.550175	8:-82623800	None:intergenic
AGTAATACATACCCCTTTGG+TGG	0.557801	8:-82624550	None:intergenic
AACAATTTACTCTTCTTCAG+TGG	0.568774	8:-82624715	None:intergenic
CACGTGAGGGAGAAGAAAGA+GGG	0.574092	8:+82623818	MsG0880047145.01.T01:CDS
TGAGGAACCAGAGGTATGCA+AGG	0.602234	8:+82624664	MsG0880047145.01.T01:CDS
ATACCCCTTTGGTGGAAGTG+TGG	0.624412	8:-82624542	None:intergenic
ATGGCATCAAGAAGCCAAAG+AGG	0.631544	8:+82624514	MsG0880047145.01.T01:CDS
TGGGAGGAGGAGATCACGTG+AGG	0.641908	8:+82623804	MsG0880047145.01.T01:CDS
GGGAGGAGGAGATCACGTGA+GGG	0.660806	8:+82623805	MsG0880047145.01.T01:CDS
CTCACAAGATAGCCTATGGG+AGG	0.668072	8:+82623788	None:intergenic
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	8:-82624481	None:intergenic
TCGCCACACTTCCACCAAAG+GGG	0.701603	8:+82624539	MsG0880047145.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAAAAAAAAAAACTTGTTA+GGG	-	Chr8:82624055-82624074	None:intergenic	10.0%
!!!	ATTTTAGTAAATAAACTATT+TGG	+	Chr8:82624192-82624211	MsG0880047145.01.T01:intron	10.0%
!!!	TAAAAAAAAAAAAACTTGTT+AGG	-	Chr8:82624056-82624075	None:intergenic	10.0%
!!!	TTTATGTTTTTAAAATTTCA+TGG	+	Chr8:82624013-82624032	MsG0880047145.01.T01:intron	10.0%
!!!	TTTTAGTAAATAAACTATTT+GGG	+	Chr8:82624193-82624212	MsG0880047145.01.T01:intron	10.0%
!!!	GCTAACAAAAATATTAATTT+TGG	+	Chr8:82624079-82624098	MsG0880047145.01.T01:intron	15.0%
!!	TTGAAAAATGAAGAAATTGA+AGG	-	Chr8:82624381-82624400	None:intergenic	20.0%
!	AAAAATGAAGAAATTGAAGG+AGG	-	Chr8:82624378-82624397	None:intergenic	25.0%
!	TTGAAATCATTGAAACAAGA+GGG	+	Chr8:82624128-82624147	MsG0880047145.01.T01:intron	25.0%
!!	TTTGAAATCATTGAAACAAG+AGG	+	Chr8:82624127-82624146	MsG0880047145.01.T01:intron	25.0%
!!!	ATTCTAATTTGAGAGCATTT+AGG	+	Chr8:82623926-82623945	MsG0880047145.01.T01:intron	25.0%
!!!	ATTTTTTTGATAACTGCAGA+TGG	+	Chr8:82624629-82624648	MsG0880047145.01.T01:intron	25.0%
	GAAGTGTGTAATGCAAAAAT+GGG	+	Chr8:82623957-82623976	MsG0880047145.01.T01:intron	30.0%
	TGAAATCATTGAAACAAGAG+GGG	+	Chr8:82624129-82624148	MsG0880047145.01.T01:intron	30.0%
	CAGAGTTAAGTGAAGATGTA+AGG	-	Chr8:82624412-82624431	None:intergenic	35.0%
	GTTAGATGATCACTGTAGTA+AGG	+	Chr8:82623847-82623866	MsG0880047145.01.T01:intron	35.0%
	TCTCTAAGTTACAGCCTAAT+GGG	+	Chr8:82624218-82624237	MsG0880047145.01.T01:intron	35.0%
	TGTGTGTTGAATTTCAGAGA+TGG	+	Chr8:82624440-82624459	MsG0880047145.01.T01:intron	35.0%
	TTCTCTAAGTTACAGCCTAA+TGG	+	Chr8:82624217-82624236	MsG0880047145.01.T01:intron	35.0%
!	AGATGGATCCAAAGTTTTTG+AGG	+	Chr8:82624646-82624665	MsG0880047145.01.T01:intron	35.0%
!	AGGAAGCACAACAAGAAAAA+CGG	+	Chr8:82624684-82624703	MsG0880047145.01.T01:CDS	35.0%
!	ATGTCTGACTCTGAAAGAAA+TGG	-	Chr8:82624270-82624289	None:intergenic	35.0%
!	GAAACCTAATTCTGAAGATG+CGG	-	Chr8:82624354-82624373	None:intergenic	35.0%
!	GGAAGTGTGTAATGCAAAAA+TGG	+	Chr8:82623956-82623975	MsG0880047145.01.T01:intron	35.0%
!!	TGAGAGCATTTAGGTTTAGA+GGG	+	Chr8:82623935-82623954	MsG0880047145.01.T01:intron	35.0%
!!	TTGAGAGCATTTAGGTTTAG+AGG	+	Chr8:82623934-82623953	MsG0880047145.01.T01:intron	35.0%
	AGATGATCACTGTAGTAAGG+TGG	+	Chr8:82623850-82623869	MsG0880047145.01.T01:intron	40.0%
	AGTAATACATACCCCTTTGG+TGG	-	Chr8:82624553-82624572	None:intergenic	40.0%
	CTGAGTAATACATACCCCTT+TGG	-	Chr8:82624556-82624575	None:intergenic	40.0%
	GAAGATGACAGAGAACTAGA+TGG	-	Chr8:82624302-82624321	None:intergenic	40.0%
	GTGTAATGCAAAAATGGGCA+GGG	+	Chr8:82623962-82623981	MsG0880047145.01.T01:intron	40.0%
	TCTTACAAGGCTCACAAGAA+TGG	+	Chr8:82624495-82624514	MsG0880047145.01.T01:CDS	40.0%
	TGTAATGCAAAAATGGGCAG+GGG	+	Chr8:82623963-82623982	MsG0880047145.01.T01:intron	40.0%
	TGTGTAATGCAAAAATGGGC+AGG	+	Chr8:82623961-82623980	MsG0880047145.01.T01:intron	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	-	Chr8:82624657-82624676	None:intergenic	40.0%
!	TGACTCTGAAAGAAATGGAC+CGG	-	Chr8:82624265-82624284	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	+	Chr8:82624655-82624674	MsG0880047145.01.T01:CDS	40.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	Chr8:82624514-82624533	MsG0880047145.01.T01:CDS	45.0%
	GCAACCGCATCTTCAGAATT+AGG	+	Chr8:82624347-82624366	MsG0880047145.01.T01:intron	45.0%
	TCACCACCACTAATCACCAA+TGG	-	Chr8:82623896-82623915	None:intergenic	45.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	Chr8:82624484-82624503	None:intergenic	45.0%
!	GACTCTGAAAGAAATGGACC+GGG	-	Chr8:82624264-82624283	None:intergenic	45.0%
!	GGTTGCACTAGGGTTTTTCT+TGG	-	Chr8:82624333-82624352	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	-	Chr8:82624494-82624513	None:intergenic	45.0%
!	TGCACTAGGGTTTTTCTTGG+CGG	-	Chr8:82624330-82624349	None:intergenic	45.0%
	ATACCCCTTTGGTGGAAGTG+TGG	-	Chr8:82624545-82624564	None:intergenic	50.0%
	ATCACCAATGGCAGCCTACA+AGG	-	Chr8:82623884-82623903	None:intergenic	50.0%
	ATCGCCACACTTCCACCAAA+GGG	+	Chr8:82624538-82624557	MsG0880047145.01.T01:CDS	50.0%
	CACGTGAGGGAGAAGAAAGA+GGG	+	Chr8:82623818-82623837	MsG0880047145.01.T01:CDS	50.0%
	CGCTCACAACCAGTCTTACA+AGG	+	Chr8:82624482-82624501	MsG0880047145.01.T01:CDS	50.0%
	CTGAAGATGCGGTTGCACTA+GGG	-	Chr8:82624343-82624362	None:intergenic	50.0%
	TCACGTGAGGGAGAAGAAAG+AGG	+	Chr8:82623817-82623836	MsG0880047145.01.T01:CDS	50.0%
	TCTGAAAGAAATGGACCGGG+TGG	-	Chr8:82624261-82624280	None:intergenic	50.0%
	TCTGAAGATGCGGTTGCACT+AGG	-	Chr8:82624344-82624363	None:intergenic	50.0%
	TGTGCTTCCTTGCATACCTC+TGG	-	Chr8:82624674-82624693	None:intergenic	50.0%
!	AGGCTGCCATTGGTGATTAG+TGG	+	Chr8:82623887-82623906	MsG0880047145.01.T01:intron	50.0%
!	CTGCCATTGGTGATTAGTGG+TGG	+	Chr8:82623890-82623909	MsG0880047145.01.T01:intron	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	+	Chr8:82624664-82624683	MsG0880047145.01.T01:CDS	50.0%
	AGGTGGCAACGACACCTTGT+AGG	+	Chr8:82623867-82623886	MsG0880047145.01.T01:intron	55.0%
	CATCGCCACACTTCCACCAA+AGG	+	Chr8:82624537-82624556	MsG0880047145.01.T01:CDS	55.0%
	GAAGTGTGGCGATGCCTCTT+TGG	-	Chr8:82624531-82624550	None:intergenic	55.0%
	GACACCTTGTAGGCTGCCAT+TGG	+	Chr8:82623877-82623896	MsG0880047145.01.T01:intron	55.0%
	TCGCCACACTTCCACCAAAG+GGG	+	Chr8:82624539-82624558	MsG0880047145.01.T01:CDS	55.0%
!	GTGAGATGGGCTTGCCCATT+AGG	-	Chr8:82624235-82624254	None:intergenic	55.0%
	AGCCCATCTCACTCTCCACC+CGG	+	Chr8:82624243-82624262	MsG0880047145.01.T01:intron	60.0%
	GACCGGGTGGAGAGTGAGAT+GGG	-	Chr8:82624248-82624267	None:intergenic	60.0%
	GGGAGGAGGAGATCACGTGA+GGG	+	Chr8:82623805-82623824	MsG0880047145.01.T01:CDS	60.0%
	TGGGAGGAGGAGATCACGTG+AGG	+	Chr8:82623804-82623823	MsG0880047145.01.T01:CDS	60.0%
	GGACCGGGTGGAGAGTGAGA+TGG	-	Chr8:82624249-82624268	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	82623803	82624731	82623803	ID=MsG0880047145.01;Name=MsG0880047145.01
Chr8	mRNA	82623803	82624731	82623803	ID=MsG0880047145.01.T01;Parent=MsG0880047145.01;Name=MsG0880047145.01.T01;_AED=0.02;_eAED=0.02;_QI=0\|0.5\|0\|0.66\|1\|1\|3\|0\|82
Chr8	exon	82623803	82623863	82623803	ID=MsG0880047145.01.T01:exon:20356;Parent=MsG0880047145.01.T01
Chr8	exon	82624457	82624560	82624457	ID=MsG0880047145.01.T01:exon:20357;Parent=MsG0880047145.01.T01
Chr8	exon	82624648	82624731	82624648	ID=MsG0880047145.01.T01:exon:20358;Parent=MsG0880047145.01.T01
Chr8	CDS	82623803	82623863	82623803	ID=MsG0880047145.01.T01:cds;Parent=MsG0880047145.01.T01
Chr8	CDS	82624457	82624560	82624457	ID=MsG0880047145.01.T01:cds;Parent=MsG0880047145.01.T01
Chr8	CDS	82624648	82624731	82624648	ID=MsG0880047145.01.T01:cds;Parent=MsG0880047145.01.T01

Gene Sequence	>MsG0880047145.01.T01 ATGGGAGGAGGAGATCACGTGAGGGAGAAGAAAGAGGGAGACGAGTTAGATGATCACTGTAAGATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAGAGGCATCGCCACACTTCCACCAAAGGGATGGATCCAAAGTTTTTGAGGAACCAGAGGTATGCAAGGAAGCACAACAAGAAAAACGGTGAAATCACCACTGAAGAAGAGTAA
Protein sequence	>MsG0880047145.01.T01 MGGGDHVREKKEGDELDDHCKMAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEITTEEE*