Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002547.01.T01 | XP_013467304.1 | 100 | 96 | 0 | 0 | 1 | 96 | 1 | 96 | 8.64E-63 | 195 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047791.01 | MsG0180002547.01 | 0.823668 | 1.223619e-53 | 1.060540e-50 |
| MsG0080048152.01 | MsG0180002547.01 | 0.833212 | 6.072980e-56 | 6.946937e-53 |
| MsG0080048383.01 | MsG0180002547.01 | 0.839666 | 1.382337e-57 | 1.922291e-54 |
| MsG0080048404.01 | MsG0180002547.01 | 0.832201 | 1.081855e-55 | 1.200709e-52 |
| MsG0080048618.01 | MsG0180002547.01 | 0.810643 | 1.040820e-50 | 6.308341e-48 |
| MsG0080048687.01 | MsG0180002547.01 | 0.809910 | 1.497890e-50 | 8.905136e-48 |
| MsG0080048774.01 | MsG0180002547.01 | 0.831963 | 1.239282e-55 | 1.365967e-52 |
| MsG0080048784.01 | MsG0180002547.01 | 0.875316 | 3.391360e-68 | 1.576200e-64 |
| MsG0080048856.01 | MsG0180002547.01 | 0.825338 | 4.949113e-54 | 4.498358e-51 |
| MsG0080048968.01 | MsG0180002547.01 | 0.807701 | 4.444700e-50 | 2.492548e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002547.01.T01 | MTR_1g048960 | 100.000 | 96 | 0 | 0 | 1 | 96 | 1 | 96 | 1.05e-66 | 195 |
Find 43 sgRNAs with CRISPR-Local
Find 174 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATAATCAGATGGATTT+GGG | 0.242746 | 1:+40196829 | MsG0180002547.01.T01:three_prime_UTR |
| AAGAGGATGGGAGGTGGACA+TGG | 0.543948 | 1:+40196619 | MsG0180002547.01.T01:CDS |
| AATATGGAAAGAAACAAATG+AGG | 0.550114 | 1:-40197097 | None:intergenic |
| AATCGAATTTGCGATAACGA+TGG | 0.704958 | 1:+40194758 | MsG0180002547.01.T01:exon |
| AATGTCCAAGCACAAATATT+TGG | 0.313332 | 1:-40196972 | None:intergenic |
| ACAATAATCAGATGGATTTG+GGG | 0.667145 | 1:+40196830 | MsG0180002547.01.T01:three_prime_UTR |
| ACTGGCGTGAAGAGGATGGG+AGG | 0.681452 | 1:+40196610 | MsG0180002547.01.T01:CDS |
| AGTAGAGGATTTCATTCAAC+TGG | 0.419375 | 1:+40196592 | MsG0180002547.01.T01:CDS |
| ATAACGATGGCATTGAACAA+CGG | 0.641834 | 1:+40194771 | MsG0180002547.01.T01:exon |
| ATATGGAAAGAAACAAATGA+GGG | 0.537796 | 1:-40197096 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAATTCCTGTTAGTCAT+TGG | + | Chr1:40195717-40195736 | MsG0180002547.01.T01:intron | 30.0% | |
| AATGTCCAAGCACAAATATT+TGG | - | Chr1:40196975-40196994 | None:intergenic | 30.0% | |
| ACTTAAGCACGAATATTCAA+TGG | + | Chr1:40196041-40196060 | MsG0180002547.01.T01:intron | 30.0% | |
| ATACAAAATATTGGCGTATG+GGG | + | Chr1:40196386-40196405 | MsG0180002547.01.T01:intron | 30.0% | |
| CAAATTAACATGGATCTTAG+AGG | + | Chr1:40195306-40195325 | MsG0180002547.01.T01:intron | 30.0% | |
| CATACAAAATATTGGCGTAT+GGG | + | Chr1:40196385-40196404 | MsG0180002547.01.T01:intron | 30.0% | |
| CCTAAGATAAATTTGAAGGT+CGG | - | Chr1:40195460-40195479 | None:intergenic | 30.0% | |
| CTTAAGCACGAATATTCAAT+GGG | + | Chr1:40196042-40196061 | MsG0180002547.01.T01:intron | 30.0% | |
| CTTGAAAGGAAATATAGACT+TGG | + | Chr1:40197002-40197021 | MsG0180002547.01.T01:three_prime_UTR | 30.0% | |
| CTTTCAATTTCGGTTACATT+AGG | + | Chr1:40194921-40194940 | MsG0180002547.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 40194679 | 40197141 | 40194679 | ID=MsG0180002547.01;Name=MsG0180002547.01 |
| Chr1 | mRNA | 40194679 | 40197141 | 40194679 | ID=MsG0180002547.01.T01;Parent=MsG0180002547.01;Name=MsG0180002547.01.T01;_AED=0.42;_eAED=0.42;_QI=98|1|1|1|1|1|2|334|96 |
| Chr1 | exon | 40194679 | 40194849 | 40194679 | ID=MsG0180002547.01.T01:exon:37059;Parent=MsG0180002547.01.T01 |
| Chr1 | exon | 40196590 | 40197141 | 40196590 | ID=MsG0180002547.01.T01:exon:37060;Parent=MsG0180002547.01.T01 |
| Chr1 | five_prime_UTR | 40194679 | 40194776 | 40194679 | ID=MsG0180002547.01.T01:five_prime_utr;Parent=MsG0180002547.01.T01 |
| Chr1 | CDS | 40194777 | 40194849 | 40194777 | ID=MsG0180002547.01.T01:cds;Parent=MsG0180002547.01.T01 |
| Chr1 | CDS | 40196590 | 40196807 | 40196590 | ID=MsG0180002547.01.T01:cds;Parent=MsG0180002547.01.T01 |
| Chr1 | three_prime_UTR | 40196808 | 40197141 | 40196808 | ID=MsG0180002547.01.T01:three_prime_utr;Parent=MsG0180002547.01.T01 |
| Gene Sequence |
>MsG0180002547.01.T01 ATGGCATTGAACAACGGTCTCAGATCTGCTGCGAAGCTAATTGCTTCTTCTGAATCCTCTATCTCCAAATCAGTGAGTAGAGGATTTCATTCAACTGGCGTGAAGAGGATGGGAGGTGGACATGGCCATAGCGAACCAGAGTATATGCATGCATATCACATGTACAACTTGGACAAGATGAAGTATCAGGGTTTGAAAATGTCTCTTGCTGTGTTCACAGCTTTCAGCATTGGTGTTGCAGTTCCTGTGTATGCAGTCATTTTCCAGCAAAAGAAGACAGCATCTGGCTGA |
| Protein sequence |
>MsG0180002547.01.T01 MALNNGLRSAAKLIASSESSISKSVSRGFHSTGVKRMGGGHGHSEPEYMHAYHMYNLDKMKYQGLKMSLAVFTAFSIGVAVPVYAVIFQQKKTASG* |
