AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004861.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004861.01.T01 MTR_1g088550 100.000 55 0 0 1 55 27 81 8.65e-34 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004861.01.T01 AT3G62810 56.604 53 23 0 1 53 27 79 5.17e-15 64.3
MsG0180004861.01.T01 AT3G62810 56.604 53 23 0 1 53 27 79 5.17e-15 64.3

Find 22 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCCTGTGCAAGAAGCTTC+TGG 0.299331 1:+83717684 None:intergenic
CGTCTCTACCATGCTCGTTC+AGG 0.394653 1:-83717645 MsG0180004861.01.T01:CDS
AATAATGATGCCGGCAGTTA+TGG 0.401922 1:-83717614 MsG0180004861.01.T01:CDS
GACGAACTCAGCGGCCTCAT+TGG 0.423868 1:+83717664 None:intergenic
AGTGAAACAAATAATCGAAC+AGG 0.460918 1:+83717563 None:intergenic
GTTCGATTATTTGTTTCACT+TGG 0.491216 1:-83717560 MsG0180004861.01.T01:CDS
AAGCTTCTGGATCTCCGCTT+CGG 0.492235 1:+83717697 None:intergenic
GATCCAGAAGCTTCTTGCAC+AGG 0.529541 1:-83717687 MsG0180004861.01.T01:CDS
GCTGAAGGGATCTGCGGCTG+AGG 0.549347 1:-83717756 MsG0180004861.01.T01:CDS
CGGCGATCATCATATGCTGA+AGG 0.569382 1:-83717771 None:intergenic
GTAAGCAGTTTGAAGAAAGC+AGG 0.570580 1:-83717730 MsG0180004861.01.T01:CDS
CTTCTGGATCTCCGCTTCGG+AGG 0.573783 1:+83717700 None:intergenic
GCACCACTCAATAATGATGC+CGG 0.582849 1:-83717623 MsG0180004861.01.T01:CDS
AGATAGAGTACCATAACTGC+CGG 0.589190 1:+83717604 None:intergenic
GAAACAAATAATCGAACAGG+TGG 0.603956 1:+83717566 None:intergenic
GGCGATCATCATATGCTGAA+GGG 0.625903 1:-83717770 None:intergenic
GCTTCTTGCACAGGCCAATG+AGG 0.635222 1:-83717678 MsG0180004861.01.T01:CDS
CTGCCGGCATCATTATTGAG+TGG 0.657290 1:+83717620 None:intergenic
CAGGAATGTCACCTCCGAAG+CGG 0.670986 1:-83717711 MsG0180004861.01.T01:CDS
GAGTGGTGCCTGAACGAGCA+TGG 0.673907 1:+83717637 None:intergenic
TCATATGCTGAAGGGATCTG+CGG 0.675279 1:-83717762 None:intergenic
CATGGTAGAGACGAACTCAG+CGG 0.745728 1:+83717655 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGTGAAACAAATAATCGAAC+AGG + Chr1:83717751-83717770 None:intergenic 30.0%
GTTCGATTATTTGTTTCACT+TGG - Chr1:83717751-83717770 MsG0180004861.01.T01:CDS 30.0%
GAAACAAATAATCGAACAGG+TGG + Chr1:83717748-83717767 None:intergenic 35.0%
AATAATGATGCCGGCAGTTA+TGG - Chr1:83717697-83717716 MsG0180004861.01.T01:CDS 40.0%
GTAAGCAGTTTGAAGAAAGC+AGG - Chr1:83717581-83717600 MsG0180004861.01.T01:CDS 40.0%
! AGATAGAGTACCATAACTGC+CGG + Chr1:83717710-83717729 None:intergenic 40.0%
GCACCACTCAATAATGATGC+CGG - Chr1:83717688-83717707 MsG0180004861.01.T01:CDS 45.0%
AAGCTTCTGGATCTCCGCTT+CGG + Chr1:83717617-83717636 None:intergenic 50.0%
CATGGTAGAGACGAACTCAG+CGG + Chr1:83717659-83717678 None:intergenic 50.0%
CTGCCGGCATCATTATTGAG+TGG + Chr1:83717694-83717713 None:intergenic 50.0%
GATCCAGAAGCTTCTTGCAC+AGG - Chr1:83717624-83717643 MsG0180004861.01.T01:CDS 50.0%
CAGGAATGTCACCTCCGAAG+CGG - Chr1:83717600-83717619 MsG0180004861.01.T01:CDS 55.0%
CGTCTCTACCATGCTCGTTC+AGG - Chr1:83717666-83717685 MsG0180004861.01.T01:CDS 55.0%
GCTTCTTGCACAGGCCAATG+AGG - Chr1:83717633-83717652 MsG0180004861.01.T01:CDS 55.0%
TGGCCTGTGCAAGAAGCTTC+TGG + Chr1:83717630-83717649 None:intergenic 55.0%
CTTCTGGATCTCCGCTTCGG+AGG + Chr1:83717614-83717633 None:intergenic 60.0%
GACGAACTCAGCGGCCTCAT+TGG + Chr1:83717650-83717669 None:intergenic 60.0%
GAGTGGTGCCTGAACGAGCA+TGG + Chr1:83717677-83717696 None:intergenic 60.0%
GCTGAAGGGATCTGCGGCTG+AGG - Chr1:83717555-83717574 MsG0180004861.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 83717553 83717780 83717553 ID=MsG0180004861.01;Name=MsG0180004861.01
Chr1 mRNA 83717553 83717780 83717553 ID=MsG0180004861.01.T01;Parent=MsG0180004861.01;Name=MsG0180004861.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|75
Chr1 exon 83717553 83717780 83717553 ID=MsG0180004861.01.T01:exon:27714;Parent=MsG0180004861.01.T01
Chr1 CDS 83717553 83717780 83717553 ID=MsG0180004861.01.T01:cds;Parent=MsG0180004861.01.T01
Gene Sequence

>MsG0180004861.01.T01

ATGCTGAAGGGATCTGCGGCTGAGGTGCGTAAGCAGTTTGAAGAAAGCAGGAATGTCACCTCCGAAGCGGAGATCCAGAAGCTTCTTGCACAGGCCAATGAGGCCGCTGAGTTCGTCTCTACCATGCTCGTTCAGGCACCACTCAATAATGATGCCGGCAGTTATGGTACTCTATCTTTCTCGTTGAGCACTCCACCTGTTCGATTATTTGTTTCACTTGGATTTTGA

Protein sequence

>MsG0180004861.01.T01

MLKGSAAEVRKQFEESRNVTSEAEIQKLLAQANEAAEFVSTMLVQAPLNNDAGSYGTLSFSLSTPPVRLFVSLGF*