AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047354.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047354.01.T01 MTR_4g125470 96.278 403 13 1 1 403 1 401 0.0 807
MsG0880047354.01.T01 MTR_5g006710 54.155 373 152 3 18 390 9 362 2.29e-148 425
MsG0880047354.01.T01 MTR_5g006670 54.324 370 150 3 21 390 8 358 1.48e-146 420
MsG0880047354.01.T01 MTR_5g006800 53.704 378 162 3 13 390 4 368 1.24e-145 418
MsG0880047354.01.T01 MTR_5g006740 54.570 372 152 5 21 390 8 364 3.12e-143 412
MsG0880047354.01.T01 MTR_5g006780 54.155 373 154 5 21 390 12 370 2.92e-141 407
MsG0880047354.01.T01 MTR_5g006750 52.957 372 160 4 21 390 12 370 7.11e-139 401
MsG0880047354.01.T01 MTR_5g008040 53.514 370 159 4 21 390 14 370 8.47e-139 401
MsG0880047354.01.T01 MTR_3g115600 53.175 378 158 5 14 390 3 362 2.50e-137 397
MsG0880047354.01.T01 MTR_5g006740 51.882 372 145 6 21 390 8 347 3.89e-128 373
MsG0880047354.01.T01 MTR_5g006720 49.738 382 139 4 21 390 11 351 5.60e-125 365
MsG0880047354.01.T01 MTR_5g006730 53.774 106 49 0 21 126 12 117 1.23e-38 135
MsG0880047354.01.T01 MTR_3g096570 73.770 61 16 0 186 246 8 68 5.27e-25 103
MsG0880047354.01.T01 MTR_3g096562 66.667 66 22 0 182 247 168 233 2.13e-23 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047354.01.T01 AT2G06050 73.766 385 97 2 11 391 2 386 0.0 604
MsG0880047354.01.T01 AT2G06050 73.766 385 97 2 11 391 2 386 0.0 604
MsG0880047354.01.T01 AT2G06050 73.766 385 97 2 11 391 2 386 0.0 604
MsG0880047354.01.T01 AT1G76690 51.989 377 168 3 14 390 7 370 2.47e-142 410
MsG0880047354.01.T01 AT1G76680 52.255 377 167 3 14 390 5 368 1.41e-140 405
MsG0880047354.01.T01 AT1G76680 49.005 402 167 4 14 390 5 393 7.44e-135 392
MsG0880047354.01.T01 AT1G09400 50.437 343 151 3 27 369 1 324 3.15e-123 360
MsG0880047354.01.T01 AT1G09400 50.704 284 121 3 86 369 1 265 4.00e-99 296
MsG0880047354.01.T01 AT1G17990 52.030 271 122 3 18 288 6 268 2.87e-98 294
MsG0880047354.01.T01 AT1G18020 52.030 271 122 3 18 288 6 268 2.87e-98 294
MsG0880047354.01.T01 AT1G17990 49.446 271 121 4 18 288 6 260 5.16e-90 273

Find 109 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATAATCCTTGACTATTTC+TGG 0.074699 8:+85186387 None:intergenic
GTGGTTCGGGCCGTTGTTTC+TGG 0.111017 8:-85186021 MsG0880047354.01.T01:CDS
TCCATCTCTCCCACTGCTTA+TGG 0.186529 8:-85187589 MsG0880047354.01.T01:CDS
TACACACAGGATCCTGTTAT+TGG 0.238161 8:-85185583 MsG0880047354.01.T01:CDS
CGGGCCGTTGTTTCTGGTAT+TGG 0.254686 8:-85186015 MsG0880047354.01.T01:CDS
CAACCTCGATATACAGCTTA+TGG 0.287602 8:-85185841 MsG0880047354.01.T01:CDS
TTAAACCTTAAAACCAAATC+TGG 0.303799 8:+85185646 None:intergenic
TCGATGCAGGTTCTTAATTC+AGG 0.309680 8:-85186043 MsG0880047354.01.T01:CDS
GGTTCTTAATTCAGGTGGTT+CGG 0.310782 8:-85186035 MsG0880047354.01.T01:CDS
TTTCATCTCTAATCCAGATT+TGG 0.311746 8:-85185659 MsG0880047354.01.T01:CDS
GATTCTGATCCACTTGGATT+AGG 0.315458 8:-85185937 MsG0880047354.01.T01:CDS
GGGTTTGGTTGTGGATGAAA+TGG 0.321666 8:+85186494 None:intergenic
CTCTTGAGTCTCATTTCCTT+TGG 0.330899 8:+85185537 None:intergenic
CCACCTCTCTAGAAGGGGTT+TGG 0.330917 8:+85186479 None:intergenic
GTTCTTAATTCAGGTGGTTC+GGG 0.333795 8:-85186034 MsG0880047354.01.T01:CDS
CCATCTCTCCCACTGCTTAT+GGG 0.338294 8:-85187588 MsG0880047354.01.T01:intron
CTCTAGAAGGGGTTTGGTTG+TGG 0.342961 8:+85186485 None:intergenic
TGTTCCTCTGAATAAATTCC+AGG 0.349073 8:+85186817 None:intergenic
CTCCTCTGAACAAGTATAAC+AGG 0.362982 8:-85185615 MsG0880047354.01.T01:CDS
GTTGGTCGTGCATCCCATTC+TGG 0.363613 8:-85186721 MsG0880047354.01.T01:intron
TTGAAGTTTGCAATCTTGTA+AGG 0.367147 8:+85187826 None:intergenic
TGTGCAGTGGTGGATATAAT+AGG 0.370705 8:-85185741 MsG0880047354.01.T01:CDS
GTGGTAAGCGCTCGAGGCTC+CGG 0.373825 8:+85186420 None:intergenic
GGTGGATATAATAGGAAGTT+AGG 0.374612 8:-85185733 MsG0880047354.01.T01:CDS
AGCTCAAGGTGATGCTGATT+TGG 0.377196 8:-85185698 MsG0880047354.01.T01:CDS
GAATTCTCCACCTCTCTAGA+AGG 0.384447 8:+85186472 None:intergenic
ATTGATCAATTCTTGAAAGA+TGG 0.389055 8:-85186108 MsG0880047354.01.T01:CDS
CGCAGGTTTCCCCATGTACC+TGG 0.393784 8:-85186835 MsG0880047354.01.T01:intron
TAATCGGTGTAGCCAATAAC+AGG 0.415841 8:+85185571 None:intergenic
GAACCTAAGAAAAGCTTATG+AGG 0.420831 8:-85185773 MsG0880047354.01.T01:CDS
GATGCTGATTTGGTATCATT+TGG 0.426648 8:-85185688 MsG0880047354.01.T01:CDS
CTGAATAAATTCCAGGTACA+TGG 0.427423 8:+85186824 None:intergenic
AATGATCGAACAGACGAGTA+TGG 0.441181 8:-85186081 MsG0880047354.01.T01:CDS
TATTCAGCAAGTGCTGCGTT+TGG 0.442605 8:+85187661 None:intergenic
AACGCTCTGCATCTCGTCAT+AGG 0.445839 8:+85187694 None:intergenic
TTTGGTGAGGAAAGGATAAT+CGG 0.449437 8:+85185555 None:intergenic
ATTGGAGTGCAGGGTGGTGT+TGG 0.449570 8:-85187719 MsG0880047354.01.T01:intron
GCTATTAATGCGATTCGAGC+AGG 0.471945 8:-85186357 MsG0880047354.01.T01:intron
TTGATCAATTCTTGAAAGAT+GGG 0.478672 8:-85186107 MsG0880047354.01.T01:CDS
CTCAAGCCAGATGGTTCCTA+TGG 0.479424 8:-85186450 MsG0880047354.01.T01:CDS
ACAAAAGATAATGCTGCCTT+TGG 0.485408 8:+85186756 None:intergenic
AGGTTGAGTAACATGAAGAT+AGG 0.492670 8:+85185858 None:intergenic
GAAACTGAGGTTACCAGAAT+GGG 0.497238 8:+85186708 None:intergenic
AGGCTCCGGGTATGCGCCAT+AGG 0.499161 8:+85186434 None:intergenic
GGTTTGGTTGTGGATGAAAT+GGG 0.499316 8:+85186495 None:intergenic
TTGATGGAGTCGAAATTCAC+GGG 0.503324 8:-85186146 MsG0880047354.01.T01:CDS
GCATCATTGTGATCAATTGC+TGG 0.504056 8:+85185964 None:intergenic
TGAAACTGAGGTTACCAGAA+TGG 0.512235 8:+85186707 None:intergenic
TTTGATGGAGTCGAAATTCA+CGG 0.512397 8:-85186147 MsG0880047354.01.T01:CDS
AGGAGAAAAGAGGGTGGTGC+TGG 0.515328 8:+85187846 None:intergenic
TGGTAAGCGCTCGAGGCTCC+GGG 0.517375 8:+85186421 None:intergenic
CCCATAAGCAGTGGGAGAGA+TGG 0.517721 8:+85187588 None:intergenic
TCATTTCCTTTGGTGAGGAA+AGG 0.518047 8:+85185547 None:intergenic
CAATCTTGTAAGGAGAAAAG+AGG 0.521873 8:+85187836 None:intergenic
GAATCGCATTAATAGCTGAT+TGG 0.525902 8:+85186364 None:intergenic
GCTATTGATTCTGATCCACT+TGG 0.530394 8:-85185943 MsG0880047354.01.T01:CDS
TATGCGCCATAGGAACCATC+TGG 0.531081 8:+85186444 None:intergenic
GTCGAAATTCACGGGGCACA+CGG 0.533112 8:-85186138 MsG0880047354.01.T01:CDS
GAGTCTCATTTCCTTTGGTG+AGG 0.533954 8:+85185542 None:intergenic
ACGAGATGCAGAGCGTTGAA+TGG 0.535942 8:-85187688 MsG0880047354.01.T01:CDS
TTCCTGTTATACTTGTTCAG+AGG 0.544474 8:+85185613 None:intergenic
AATTCTCCACCTCTCTAGAA+GGG 0.550426 8:+85186473 None:intergenic
TCGAAATTCACGGGGCACAC+GGG 0.550944 8:-85186137 MsG0880047354.01.T01:CDS
TGAATAAATTCCAGGTACAT+GGG 0.553359 8:+85186825 None:intergenic
TGGTTCCTATGGCGCATACC+CGG 0.554696 8:-85186439 MsG0880047354.01.T01:CDS
GTATGACAAGGAAGAAGAAG+AGG 0.555686 8:-85185809 MsG0880047354.01.T01:CDS
GAAATGGGTGCAGCACCACC+AGG 0.555752 8:+85186510 None:intergenic
CAGAGGAACAAGTGGAGGCA+TGG 0.561445 8:-85186804 MsG0880047354.01.T01:CDS
GGTGGATTTCTCATCGGTGA+AGG 0.575119 8:-85187616 MsG0880047354.01.T01:CDS
GATCACTTGAAAATCGATGC+AGG 0.580483 8:-85186056 MsG0880047354.01.T01:CDS
ATTATATACCCATAAGCAGT+GGG 0.587040 8:+85187580 None:intergenic
ATGCAGGTTCTTAATTCAGG+TGG 0.588415 8:-85186040 MsG0880047354.01.T01:CDS
TCAGAAGTGGTAAGCGCTCG+AGG 0.590830 8:+85186414 None:intergenic
ATATAATAGGAAGTTAGGAA+TGG 0.592051 8:-85185728 MsG0880047354.01.T01:CDS
GGAATGGAAGCTGTAGCTCA+AGG 0.592444 8:-85185712 MsG0880047354.01.T01:CDS
CCAAACCCCTTCTAGAGAGG+TGG 0.592960 8:-85186479 MsG0880047354.01.T01:CDS
AGTTCAACAACACAAGTACA+AGG 0.594788 8:-85187874 MsG0880047354.01.T01:CDS
TATTTCTGGTATCTCAGAAG+TGG 0.602504 8:+85186401 None:intergenic
TACGCTCAAAGATCAACTCC+AGG 0.602812 8:-85187637 MsG0880047354.01.T01:CDS
GATTATCCTTTCCTCACCAA+AGG 0.604147 8:-85185553 MsG0880047354.01.T01:CDS
GGAACATTCATGTGCAGTGG+TGG 0.609749 8:-85185751 MsG0880047354.01.T01:CDS
TTATTCAGAGGAACAAGTGG+AGG 0.610128 8:-85186809 MsG0880047354.01.T01:CDS
AATTTATTCAGAGGAACAAG+TGG 0.610952 8:-85186812 MsG0880047354.01.T01:CDS
TTTCCATAAGCTGTATATCG+AGG 0.611276 8:+85185838 None:intergenic
ATAAGCAGTGGGAGAGATGG+AGG 0.611650 8:+85187591 None:intergenic
AAGAAGAGGCTCATTTGATG+AGG 0.613862 8:-85185795 MsG0880047354.01.T01:CDS
CAACCAAACCCCTTCTAGAG+AGG 0.615926 8:-85186482 MsG0880047354.01.T01:CDS
ATTCTCCACCTCTCTAGAAG+GGG 0.617495 8:+85186474 None:intergenic
AACCTAAGAAAAGCTTATGA+GGG 0.618035 8:-85185772 MsG0880047354.01.T01:CDS
TGGAGAATTCTCAAGCCAGA+TGG 0.619198 8:-85186459 MsG0880047354.01.T01:CDS
GGTATTGGAGCTGAAAGAGT+TGG 0.624730 8:-85186000 MsG0880047354.01.T01:CDS
GAATAAATTCCAGGTACATG+GGG 0.626230 8:+85186826 None:intergenic
ACTCCAGGTGGATTTCTCAT+CGG 0.631444 8:-85187622 MsG0880047354.01.T01:CDS
AATTATATACCCATAAGCAG+TGG 0.632930 8:+85187579 None:intergenic
ACTGCTAAGCCTAATCCAAG+TGG 0.645958 8:+85185928 None:intergenic
AGCTCCAATACCAGAAACAA+CGG 0.648152 8:+85186011 None:intergenic
TGTGAAGCAGTGTATCAGCC+TGG 0.649524 8:-85186528 MsG0880047354.01.T01:intron
GTGGATGCTGCTCATGCCAA+AGG 0.652152 8:-85186772 MsG0880047354.01.T01:CDS
GATCGAACAGACGAGTATGG+TGG 0.652328 8:-85186078 MsG0880047354.01.T01:CDS
TGAGATACCAGAAATAGTCA+AGG 0.658712 8:-85186394 MsG0880047354.01.T01:CDS
TGTACCTGGAATTTATTCAG+AGG 0.660053 8:-85186821 MsG0880047354.01.T01:CDS
TCACCGATGAGAAATCCACC+TGG 0.665351 8:+85187619 None:intergenic
AATCTTGTAAGGAGAAAAGA+GGG 0.669109 8:+85187837 None:intergenic
GGAGGCATGGAGAAATGTCG+TGG 0.680628 8:-85186791 MsG0880047354.01.T01:CDS
GCTCAAAGATCAACTCCAGG+TGG 0.683876 8:-85187634 MsG0880047354.01.T01:CDS
GAGGGAACATTCATGTGCAG+TGG 0.698747 8:-85185754 MsG0880047354.01.T01:CDS
TGATGGAGTCGAAATTCACG+GGG 0.717446 8:-85186145 MsG0880047354.01.T01:CDS
GAAGCAGTGTATCAGCCTGG+TGG 0.735693 8:-85186525 MsG0880047354.01.T01:intron
CTTGTAAGGAGAAAAGAGGG+TGG 0.760384 8:+85187840 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATGAAAATATTACATTA+TGG - Chr8:85186531-85186550 MsG0880047354.01.T01:intron 10.0%
!!! TGTAATATTTTCATATATTT+AGG + Chr8:85186528-85186547 None:intergenic 10.0%
!! AAACAGTTATAAAGTAATAA+AGG - Chr8:85186062-85186081 MsG0880047354.01.T01:CDS 15.0%
!! AGTACATAAAACTAAATAAA+CGG - Chr8:85186300-85186319 MsG0880047354.01.T01:intron 15.0%
!! TATATAAAAGAAATATAGGA+CGG - Chr8:85186138-85186157 MsG0880047354.01.T01:CDS 15.0%
!!! ATAAAGTAATAAAGGTATAT+TGG - Chr8:85186070-85186089 MsG0880047354.01.T01:CDS 15.0%
!!! CAAATTGGATTATTTTTTTA+AGG + Chr8:85186006-85186025 None:intergenic 15.0%
!!! TATTTTCATATATTTAGGAT+TGG + Chr8:85186523-85186542 None:intergenic 15.0%
!! AAATAATATTATGTTGATGC+TGG - Chr8:85185959-85185978 MsG0880047354.01.T01:CDS 20.0%
!! ACATTATGGTATTGTAATAT+AGG - Chr8:85186545-85186564 MsG0880047354.01.T01:intron 20.0%
!! CATTATGGTATTGTAATATA+GGG - Chr8:85186546-85186565 MsG0880047354.01.T01:intron 20.0%
!!! TTTATCCTTTGAAATTTGTA+AGG + Chr8:85186115-85186134 None:intergenic 20.0%
! AAAGAAAAAGCTTGAAAAAG+AGG + Chr8:85185861-85185880 None:intergenic 25.0%
! ATATAATAGGAAGTTAGGAA+TGG - Chr8:85187686-85187705 MsG0880047354.01.T01:CDS 25.0%
! ATTGATCAATTCTTGAAAGA+TGG - Chr8:85187306-85187325 MsG0880047354.01.T01:intron 25.0%
! ATTGTCCTTACAAATTTCAA+AGG - Chr8:85186107-85186126 MsG0880047354.01.T01:CDS 25.0%
! CATACATCTAACAATCAAAT+TGG + Chr8:85186021-85186040 None:intergenic 25.0%
! CTCACAAACATAAAATAGTA+AGG + Chr8:85187125-85187144 None:intergenic 25.0%
! GACATCAATGAATATAAGTT+AGG + Chr8:85185912-85185931 None:intergenic 25.0%
! TATTTGTTTACATCTCTTCA+AGG - Chr8:85187085-85187104 MsG0880047354.01.T01:intron 25.0%
! TGTATGTTGGTATTTACTAT+TGG - Chr8:85186035-85186054 MsG0880047354.01.T01:CDS 25.0%
! TTAAACCTTAAAACCAAATC+TGG + Chr8:85187771-85187790 None:intergenic 25.0%
! TTCACAATTAAATCATTGAG+TGG + Chr8:85186872-85186891 None:intergenic 25.0%
! TTGATCAATTCTTGAAAGAT+GGG - Chr8:85187307-85187326 MsG0880047354.01.T01:intron 25.0%
! TTGTAATATAGGGAAATGTT+TGG - Chr8:85186556-85186575 MsG0880047354.01.T01:intron 25.0%
! TTTGATTGTTAGATGTATGT+TGG - Chr8:85186022-85186041 MsG0880047354.01.T01:CDS 25.0%
!!! GTTTTTTTCTCTGTTATTGT+GGG - Chr8:85185646-85185665 MsG0880047354.01.T01:CDS 25.0%
!!! TGTTTTTTTCTCTGTTATTG+TGG - Chr8:85185645-85185664 MsG0880047354.01.T01:CDS 25.0%
AACCTAAGAAAAGCTTATGA+GGG - Chr8:85187642-85187661 MsG0880047354.01.T01:CDS 30.0%
AATCTTGTAAGGAGAAAAGA+GGG + Chr8:85185580-85185599 None:intergenic 30.0%
AATTATATACCCATAAGCAG+TGG + Chr8:85185838-85185857 None:intergenic 30.0%
AATTTATTCAGAGGAACAAG+TGG - Chr8:85186602-85186621 MsG0880047354.01.T01:intron 30.0%
ATTATATACCCATAAGCAGT+GGG + Chr8:85185837-85185856 None:intergenic 30.0%
GAATAGTACTAAAACCATGT+AGG + Chr8:85186339-85186358 None:intergenic 30.0%
TGAATAAATTCCAGGTACAT+GGG + Chr8:85186592-85186611 None:intergenic 30.0%
TTGAAGTTTGCAATCTTGTA+AGG + Chr8:85185591-85185610 None:intergenic 30.0%
TTTCATCTCTAATCCAGATT+TGG - Chr8:85187755-85187774 MsG0880047354.01.T01:intron 30.0%
! CAAACTTCAACTTTTCTCAT+AGG - Chr8:85185601-85185620 MsG0880047354.01.T01:CDS 30.0%
! GCATTATCTTTTGTCAGTTA+TGG - Chr8:85186667-85186686 MsG0880047354.01.T01:intron 30.0%
! GTTTTCAATGCAGTTCATTA+AGG + Chr8:85186759-85186778 None:intergenic 30.0%
!!! CTAATCCAGATTTGGTTTTA+AGG - Chr8:85187763-85187782 MsG0880047354.01.T01:intron 30.0%
AACAATCTCCAAAAAGAGCT+TGG - Chr8:85187522-85187541 MsG0880047354.01.T01:intron 35.0%
AAGTTGCGACAAATGAATCA+AGG + Chr8:85186237-85186256 None:intergenic 35.0%
ACAAAAGATAATGCTGCCTT+TGG + Chr8:85186661-85186680 None:intergenic 35.0%
ACTATGATCTGATGTGGTTA+TGG - Chr8:85185671-85185690 MsG0880047354.01.T01:CDS 35.0%
AGGTTGAGTAACATGAAGAT+AGG + Chr8:85187559-85187578 None:intergenic 35.0%
AGTTCAACAACACAAGTACA+AGG - Chr8:85185540-85185559 MsG0880047354.01.T01:CDS 35.0%
AGTTGCGACAAATGAATCAA+GGG + Chr8:85186236-85186255 None:intergenic 35.0%
ATCAACGACAAAAAGAACAC+AGG + Chr8:85186417-85186436 None:intergenic 35.0%
ATCTGATGTGGTTATGGTAT+TGG - Chr8:85185677-85185696 MsG0880047354.01.T01:CDS 35.0%
ATGAAATGAGACGTACATAC+AGG - Chr8:85187243-85187262 MsG0880047354.01.T01:intron 35.0%
CAATCTTGTAAGGAGAAAAG+AGG + Chr8:85185581-85185600 None:intergenic 35.0%
CGATAATCCTTGACTATTTC+TGG + Chr8:85187030-85187049 None:intergenic 35.0%
CTGAATAAATTCCAGGTACA+TGG + Chr8:85186593-85186612 None:intergenic 35.0%
GAACCTAAGAAAAGCTTATG+AGG - Chr8:85187641-85187660 MsG0880047354.01.T01:CDS 35.0%
GAATAAATTCCAGGTACATG+GGG + Chr8:85186591-85186610 None:intergenic 35.0%
GAATCGCATTAATAGCTGAT+TGG + Chr8:85187053-85187072 None:intergenic 35.0%
GGTGGATATAATAGGAAGTT+AGG - Chr8:85187681-85187700 MsG0880047354.01.T01:CDS 35.0%
GTCAAACAAAAAGTCCTACA+TGG - Chr8:85186322-85186341 MsG0880047354.01.T01:intron 35.0%
TGAGATACCAGAAATAGTCA+AGG - Chr8:85187020-85187039 MsG0880047354.01.T01:intron 35.0%
TGGAAAAACTGAGTATGACA+AGG - Chr8:85187593-85187612 MsG0880047354.01.T01:CDS 35.0%
TGTACCTGGAATTTATTCAG+AGG - Chr8:85186593-85186612 MsG0880047354.01.T01:intron 35.0%
TGTTCCTCTGAATAAATTCC+AGG + Chr8:85186600-85186619 None:intergenic 35.0%
TTAAACACAATCAGCCTCAT+TGG + Chr8:85186374-85186393 None:intergenic 35.0%
TTCCTGTTATACTTGTTCAG+AGG + Chr8:85187804-85187823 None:intergenic 35.0%
TTTCCATAAGCTGTATATCG+AGG + Chr8:85187579-85187598 None:intergenic 35.0%
TTTGATGGAGTCGAAATTCA+CGG - Chr8:85187267-85187286 MsG0880047354.01.T01:intron 35.0%
TTTGGTGAGGAAAGGATAAT+CGG + Chr8:85187862-85187881 None:intergenic 35.0%
! CATGCATCTGATTTTGTCAA+TGG - Chr8:85186203-85186222 MsG0880047354.01.T01:intron 35.0%
! CATTTAGGATAGTGAAACTG+AGG + Chr8:85186722-85186741 None:intergenic 35.0%
! GATGAACTTGTGAAGCATTT+AGG + Chr8:85186737-85186756 None:intergenic 35.0%
! GTTTTCTACCAAGCTCTTTT+TGG + Chr8:85187533-85187552 None:intergenic 35.0%
! TTCCCTCATAAGCTTTTCTT+AGG + Chr8:85187647-85187666 None:intergenic 35.0%
! TTTTGTCAGTTATGGCATGT+TGG - Chr8:85186675-85186694 MsG0880047354.01.T01:intron 35.0%
!! GATGCTGATTTGGTATCATT+TGG - Chr8:85187726-85187745 MsG0880047354.01.T01:intron 35.0%
!! TATTTCTGGTATCTCAGAAG+TGG + Chr8:85187016-85187035 None:intergenic 35.0%
AAGAAGAGGCTCATTTGATG+AGG - Chr8:85187619-85187638 MsG0880047354.01.T01:CDS 40.0%
AATGATCGAACAGACGAGTA+TGG - Chr8:85187333-85187352 MsG0880047354.01.T01:intron 40.0%
AGCTCCAATACCAGAAACAA+CGG + Chr8:85187406-85187425 None:intergenic 40.0%
ATGCAGGTTCTTAATTCAGG+TGG - Chr8:85187374-85187393 MsG0880047354.01.T01:intron 40.0%
CAACCTCGATATACAGCTTA+TGG - Chr8:85187573-85187592 MsG0880047354.01.T01:intron 40.0%
CTCCTCTGAACAAGTATAAC+AGG - Chr8:85187799-85187818 MsG0880047354.01.T01:intron 40.0%
CTCTTGAGTCTCATTTCCTT+TGG + Chr8:85187880-85187899 None:intergenic 40.0%
GAAACTGAGGTTACCAGAAT+GGG + Chr8:85186709-85186728 None:intergenic 40.0%
GATCACTTGAAAATCGATGC+AGG - Chr8:85187358-85187377 MsG0880047354.01.T01:intron 40.0%
GATTATCCTTTCCTCACCAA+AGG - Chr8:85187861-85187880 MsG0880047354.01.T01:CDS 40.0%
GATTCTGATCCACTTGGATT+AGG - Chr8:85187477-85187496 MsG0880047354.01.T01:intron 40.0%
GCATCATTGTGATCAATTGC+TGG + Chr8:85187453-85187472 None:intergenic 40.0%
GGTTCTTAATTCAGGTGGTT+CGG - Chr8:85187379-85187398 MsG0880047354.01.T01:intron 40.0%
GTATGACAAGGAAGAAGAAG+AGG - Chr8:85187605-85187624 MsG0880047354.01.T01:CDS 40.0%
GTTCTTAATTCAGGTGGTTC+GGG - Chr8:85187380-85187399 MsG0880047354.01.T01:intron 40.0%
TAATCGGTGTAGCCAATAAC+AGG + Chr8:85187846-85187865 None:intergenic 40.0%
TACACACAGGATCCTGTTAT+TGG - Chr8:85187831-85187850 MsG0880047354.01.T01:CDS 40.0%
TCATTTCCTTTGGTGAGGAA+AGG + Chr8:85187870-85187889 None:intergenic 40.0%
TCGATGCAGGTTCTTAATTC+AGG - Chr8:85187371-85187390 MsG0880047354.01.T01:intron 40.0%
TGAAACTGAGGTTACCAGAA+TGG + Chr8:85186710-85186729 None:intergenic 40.0%
TTATTCAGAGGAACAAGTGG+AGG - Chr8:85186605-85186624 MsG0880047354.01.T01:intron 40.0%
TTGATGGAGTCGAAATTCAC+GGG - Chr8:85187268-85187287 MsG0880047354.01.T01:intron 40.0%
! AATTCTCCACCTCTCTAGAA+GGG + Chr8:85186944-85186963 None:intergenic 40.0%
! CAGGAAGACATTTTACACAC+AGG - Chr8:85187818-85187837 MsG0880047354.01.T01:CDS 40.0%
! CATCTGATTTTGTCAATGGC+CGG - Chr8:85186207-85186226 MsG0880047354.01.T01:intron 40.0%
! GGTTTGGTTGTGGATGAAAT+GGG + Chr8:85186922-85186941 None:intergenic 40.0%
!! GACGTACATACAGGTTTTGA+TGG - Chr8:85187252-85187271 MsG0880047354.01.T01:intron 40.0%
!! GCTATTGATTCTGATCCACT+TGG - Chr8:85187471-85187490 MsG0880047354.01.T01:intron 40.0%
!! TGTGCAGTGGTGGATATAAT+AGG - Chr8:85187673-85187692 MsG0880047354.01.T01:CDS 40.0%
ACAAATGAATCAAGGGTGGC+CGG + Chr8:85186229-85186248 None:intergenic 45.0%
ACACAATCAGCCTCATTGGT+TGG + Chr8:85186370-85186389 None:intergenic 45.0%
ACTGCTAAGCCTAATCCAAG+TGG + Chr8:85187489-85187508 None:intergenic 45.0%
ATACTCGTGTTGCATCTGCA+CGG - Chr8:85187169-85187188 MsG0880047354.01.T01:intron 45.0%
CTTGTAAGGAGAAAAGAGGG+TGG + Chr8:85185577-85185596 None:intergenic 45.0%
GAATTCTCCACCTCTCTAGA+AGG + Chr8:85186945-85186964 None:intergenic 45.0%
GAGTCTCATTTCCTTTGGTG+AGG + Chr8:85187875-85187894 None:intergenic 45.0%
GCTATTAATGCGATTCGAGC+AGG - Chr8:85187057-85187076 MsG0880047354.01.T01:intron 45.0%
TACGCTCAAAGATCAACTCC+AGG - Chr8:85185777-85185796 MsG0880047354.01.T01:CDS 45.0%
TATAGGGAAATGTTTGGCGC+AGG - Chr8:85186562-85186581 MsG0880047354.01.T01:intron 45.0%
TCTATCGCTACCAACCAATG+AGG - Chr8:85186357-85186376 MsG0880047354.01.T01:intron 45.0%
TGATGGAGTCGAAATTCACG+GGG - Chr8:85187269-85187288 MsG0880047354.01.T01:intron 45.0%
TGCGACAAATGAATCAAGGG+TGG + Chr8:85186233-85186252 None:intergenic 45.0%
TGGAGAATTCTCAAGCCAGA+TGG - Chr8:85186955-85186974 MsG0880047354.01.T01:intron 45.0%
TGGGTGACTATGATCTGATG+TGG - Chr8:85185665-85185684 MsG0880047354.01.T01:CDS 45.0%
! ACTCCAGGTGGATTTCTCAT+CGG - Chr8:85185792-85185811 MsG0880047354.01.T01:CDS 45.0%
! AGCTCAAGGTGATGCTGATT+TGG - Chr8:85187716-85187735 MsG0880047354.01.T01:intron 45.0%
! ATTCTCCACCTCTCTAGAAG+GGG + Chr8:85186943-85186962 None:intergenic 45.0%
! GGGTTTGGTTGTGGATGAAA+TGG + Chr8:85186923-85186942 None:intergenic 45.0%
! GGTATTGGAGCTGAAAGAGT+TGG - Chr8:85187414-85187433 MsG0880047354.01.T01:intron 45.0%
! GGTTATGGTATTGGAGTGCA+GGG - Chr8:85185686-85185705 MsG0880047354.01.T01:CDS 45.0%
! TATTCAGCAAGTGCTGCGTT+TGG + Chr8:85185756-85185775 None:intergenic 45.0%
! TGGTTATGGTATTGGAGTGC+AGG - Chr8:85185685-85185704 MsG0880047354.01.T01:CDS 45.0%
!! AAAATATATAAAAGAAATAT+AGG - Chr8:85186134-85186153 MsG0880047354.01.T01:CDS 5.0%
AACGCTCTGCATCTCGTCAT+AGG + Chr8:85185723-85185742 None:intergenic 50.0%
ATAAGCAGTGGGAGAGATGG+AGG + Chr8:85185826-85185845 None:intergenic 50.0%
CCATCTCTCCCACTGCTTAT+GGG - Chr8:85185826-85185845 MsG0880047354.01.T01:CDS 50.0%
CTCAAGCCAGATGGTTCCTA+TGG - Chr8:85186964-85186983 MsG0880047354.01.T01:intron 50.0%
GAGGGAACATTCATGTGCAG+TGG - Chr8:85187660-85187679 MsG0880047354.01.T01:CDS 50.0%
GATCGAACAGACGAGTATGG+TGG - Chr8:85187336-85187355 MsG0880047354.01.T01:intron 50.0%
GCTCAAAGATCAACTCCAGG+TGG - Chr8:85185780-85185799 MsG0880047354.01.T01:CDS 50.0%
GGAACATTCATGTGCAGTGG+TGG - Chr8:85187663-85187682 MsG0880047354.01.T01:CDS 50.0%
GGAATGGAAGCTGTAGCTCA+AGG - Chr8:85187702-85187721 MsG0880047354.01.T01:CDS 50.0%
GTGCAGATGCAACACGAGTA+TGG + Chr8:85187170-85187189 None:intergenic 50.0%
TACTCGTGTTGCATCTGCAC+GGG - Chr8:85187170-85187189 MsG0880047354.01.T01:intron 50.0%
TATGCGCCATAGGAACCATC+TGG + Chr8:85186973-85186992 None:intergenic 50.0%
TCACCGATGAGAAATCCACC+TGG + Chr8:85185798-85185817 None:intergenic 50.0%
TCCATCTCTCCCACTGCTTA+TGG - Chr8:85185825-85185844 MsG0880047354.01.T01:CDS 50.0%
TGTGAAGCAGTGTATCAGCC+TGG - Chr8:85186886-85186905 MsG0880047354.01.T01:intron 50.0%
TGTTGCATCTGCACGGGTAT+GGG - Chr8:85187176-85187195 MsG0880047354.01.T01:intron 50.0%
! CAACCAAACCCCTTCTAGAG+AGG - Chr8:85186932-85186951 MsG0880047354.01.T01:intron 50.0%
! CTCTAGAAGGGGTTTGGTTG+TGG + Chr8:85186932-85186951 None:intergenic 50.0%
! GGTGGATTTCTCATCGGTGA+AGG - Chr8:85185798-85185817 MsG0880047354.01.T01:CDS 50.0%
! TATGGTATTGGAGTGCAGGG+TGG - Chr8:85185689-85185708 MsG0880047354.01.T01:CDS 50.0%
!! ACGAGATGCAGAGCGTTGAA+TGG - Chr8:85185726-85185745 MsG0880047354.01.T01:CDS 50.0%
AGGAGAAAAGAGGGTGGTGC+TGG + Chr8:85185571-85185590 None:intergenic 55.0%
ATTGGAGTGCAGGGTGGTGT+TGG - Chr8:85185695-85185714 MsG0880047354.01.T01:CDS 55.0%
CAGAGGAACAAGTGGAGGCA+TGG - Chr8:85186610-85186629 MsG0880047354.01.T01:intron 55.0%
CCCATAAGCAGTGGGAGAGA+TGG + Chr8:85185829-85185848 None:intergenic 55.0%
GAAGCAGTGTATCAGCCTGG+TGG - Chr8:85186889-85186908 MsG0880047354.01.T01:intron 55.0%
GTCGAAATTCACGGGGCACA+CGG - Chr8:85187276-85187295 MsG0880047354.01.T01:intron 55.0%
GTGGATGCTGCTCATGCCAA+AGG - Chr8:85186642-85186661 MsG0880047354.01.T01:intron 55.0%
GTGTTGCATCTGCACGGGTA+TGG - Chr8:85187175-85187194 MsG0880047354.01.T01:intron 55.0%
GTTGGTCGTGCATCCCATTC+TGG - Chr8:85186693-85186712 MsG0880047354.01.T01:intron 55.0%
TCAGAAGTGGTAAGCGCTCG+AGG + Chr8:85187003-85187022 None:intergenic 55.0%
TCGAAATTCACGGGGCACAC+GGG - Chr8:85187277-85187296 MsG0880047354.01.T01:intron 55.0%
TGGTTCCTATGGCGCATACC+CGG - Chr8:85186975-85186994 MsG0880047354.01.T01:intron 55.0%
! CCAAACCCCTTCTAGAGAGG+TGG - Chr8:85186935-85186954 MsG0880047354.01.T01:intron 55.0%
! CCACCTCTCTAGAAGGGGTT+TGG + Chr8:85186938-85186957 None:intergenic 55.0%
! GGAGGCATGGAGAAATGTCG+TGG - Chr8:85186623-85186642 MsG0880047354.01.T01:intron 55.0%
!! CGGGCCGTTGTTTCTGGTAT+TGG - Chr8:85187399-85187418 MsG0880047354.01.T01:intron 55.0%
CGCAGGTTTCCCCATGTACC+TGG - Chr8:85186579-85186598 MsG0880047354.01.T01:intron 60.0%
GAAATGGGTGCAGCACCACC+AGG + Chr8:85186907-85186926 None:intergenic 60.0%
! GTGGTTCGGGCCGTTGTTTC+TGG - Chr8:85187393-85187412 MsG0880047354.01.T01:intron 60.0%
AGGCTCCGGGTATGCGCCAT+AGG + Chr8:85186983-85187002 None:intergenic 65.0%
GTGGTAAGCGCTCGAGGCTC+CGG + Chr8:85186997-85187016 None:intergenic 65.0%
TGGTAAGCGCTCGAGGCTCC+GGG + Chr8:85186996-85187015 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 85185519 85187917 85185519 ID=MsG0880047354.01;Name=MsG0880047354.01
Chr8 mRNA 85185519 85187917 85185519 ID=MsG0880047354.01.T01;Parent=MsG0880047354.01;Name=MsG0880047354.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|5|0|403
Chr8 exon 85187814 85187917 85187814 ID=MsG0880047354.01.T01:exon:1893;Parent=MsG0880047354.01.T01
Chr8 exon 85187589 85187729 85187589 ID=MsG0880047354.01.T01:exon:1892;Parent=MsG0880047354.01.T01
Chr8 exon 85186722 85186852 85186722 ID=MsG0880047354.01.T01:exon:1891;Parent=MsG0880047354.01.T01
Chr8 exon 85186358 85186540 85186358 ID=MsG0880047354.01.T01:exon:1890;Parent=MsG0880047354.01.T01
Chr8 exon 85185519 85186171 85185519 ID=MsG0880047354.01.T01:exon:1889;Parent=MsG0880047354.01.T01
Chr8 CDS 85187814 85187917 85187814 ID=MsG0880047354.01.T01:cds;Parent=MsG0880047354.01.T01
Chr8 CDS 85187589 85187729 85187589 ID=MsG0880047354.01.T01:cds;Parent=MsG0880047354.01.T01
Chr8 CDS 85186722 85186852 85186722 ID=MsG0880047354.01.T01:cds;Parent=MsG0880047354.01.T01
Chr8 CDS 85186358 85186540 85186358 ID=MsG0880047354.01.T01:cds;Parent=MsG0880047354.01.T01
Chr8 CDS 85185519 85186171 85185519 ID=MsG0880047354.01.T01:cds;Parent=MsG0880047354.01.T01
Gene Sequence

>MsG0880047354.01.T01

ATGGCAGAGTACAATAATTCAAGTTCAACAACACAAGTACAAGGAGACACCAGCACCACCCTCTTTTCTCCTTACAAGATTGCAAACTTCAACTTTTCTCATAGGGTGGTGTTGGCACCTATGACGAGATGCAGAGCGTTGAATGGTATTCCAAACGCAGCACTTGCTGAATACTACGCTCAAAGATCAACTCCAGGTGGATTTCTCATCGGTGAAGGAACCTCCATCTCTCCCACTGCTTATGGGTTTCCCCATGTACCTGGAATTTATTCAGAGGAACAAGTGGAGGCATGGAGAAATGTCGTGGATGCTGCTCATGCCAAAGGCAGCATTATCTTTTGTCAGTTATGGCATGTTGGTCGTGCATCCCATTCTGTGTATCAGCCTGGTGGTGCTGCACCCATTTCATCCACAACCAAACCCCTTCTAGAGAGGTGGAGAATTCTCAAGCCAGATGGTTCCTATGGCGCATACCCGGAGCCTCGAGCGCTTACCACTTCTGAGATACCAGAAATAGTCAAGGATTATCGCCAATCAGCTATTAATGCGATTCGAGCAGGTTTTGATGGAGTCGAAATTCACGGGGCACACGGGTATCTGATTGATCAATTCTTGAAAGATGGGATCAATGATCGAACAGACGAGTATGGTGGATCACTTGAAAATCGATGCAGGTTCTTAATTCAGGTGGTTCGGGCCGTTGTTTCTGGTATTGGAGCTGAAAGAGTTGGTGTCAGAATTTCACCAGCAATTGATCACAATGATGCTATTGATTCTGATCCACTTGGATTAGGCTTAGCAGTGATTGAGAGACTAAACAATCTCCAAAAAGAGCTTGGTAGAAAACTCACCTATCTTCATGTTACTCAACCTCGATATACAGCTTATGGAAAAACTGAGTATGACAAGGAAGAAGAAGAGGCTCATTTGATGAGGAACCTAAGAAAAGCTTATGAGGGAACATTCATGTGCAGTGGTGGATATAATAGGAAGTTAGGAATGGAAGCTGTAGCTCAAGGTGATGCTGATTTGGTATCATTTGGTCGTCTTTTCATCTCTAATCCAGATTTGGTTTTAAGGTTTAAGCTAAATGCTCCTCTGAACAAGTATAACAGGAAGACATTTTACACACAGGATCCTGTTATTGGCTACACCGATTATCCTTTCCTCACCAAAGGAAATGAGACTCAAGAGCTAAATGCACGCCTTTGA
Protein sequence

>MsG0880047354.01.T01

MAEYNNSSSTTQVQGDTSTTLFSPYKIANFNFSHRVVLAPMTRCRALNGIPNAALAEYYAQRSTPGGFLIGEGTSISPTAYGFPHVPGIYSEEQVEAWRNVVDAAHAKGSIIFCQLWHVGRASHSVYQPGGAAPISSTTKPLLERWRILKPDGSYGAYPEPRALTTSEIPEIVKDYRQSAINAIRAGFDGVEIHGAHGYLIDQFLKDGINDRTDEYGGSLENRCRFLIQVVRAVVSGIGAERVGVRISPAIDHNDAIDSDPLGLGLAVIERLNNLQKELGRKLTYLHVTQPRYTAYGKTEYDKEEEEAHLMRNLRKAYEGTFMCSGGYNRKLGMEAVAQGDADLVSFGRLFISNPDLVLRFKLNAPLNKYNRKTFYTQDPVIGYTDYPFLTKGNETQELNARL*