Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047563.01.T01 | XP_003610256.1 | 93.302 | 642 | 7 | 4 | 1 | 608 | 1 | 640 | 0 | 1208 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047563.01.T01 | MTR_4g130210 | 93.302 | 642 | 7 | 4 | 1 | 608 | 1 | 640 | 0.0 | 1208 |
| MsG0880047563.01.T01 | MTR_4g130210 | 92.125 | 546 | 9 | 2 | 97 | 608 | 29 | 574 | 0.0 | 1018 |
| MsG0880047563.01.T01 | MTR_7g018200 | 62.047 | 635 | 167 | 11 | 16 | 608 | 24 | 626 | 0.0 | 731 |
| MsG0880047563.01.T01 | MTR_0049s0070 | 59.783 | 644 | 190 | 11 | 5 | 608 | 7 | 621 | 0.0 | 700 |
| MsG0880047563.01.T01 | MTR_6g016495 | 60.514 | 623 | 175 | 11 | 28 | 608 | 32 | 625 | 0.0 | 676 |
| MsG0880047563.01.T01 | MTR_0049s0070 | 60.410 | 586 | 164 | 10 | 63 | 608 | 1 | 558 | 0.0 | 646 |
| MsG0880047563.01.T01 | MTR_5g033820 | 56.822 | 623 | 198 | 11 | 28 | 608 | 32 | 625 | 0.0 | 642 |
| MsG0880047563.01.T01 | MTR_7g018200 | 63.960 | 505 | 133 | 9 | 16 | 478 | 24 | 521 | 0.0 | 608 |
| MsG0880047563.01.T01 | MTR_6g016495 | 62.880 | 493 | 136 | 8 | 28 | 478 | 32 | 519 | 0.0 | 560 |
| MsG0880047563.01.T01 | MTR_2g016500 | 50.080 | 623 | 232 | 11 | 28 | 608 | 37 | 622 | 0.0 | 558 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047563.01.T01 | AT2G23950 | 66.459 | 641 | 157 | 9 | 5 | 608 | 15 | 634 | 0.0 | 830 |
| MsG0880047563.01.T01 | AT4G30520 | 67.707 | 641 | 160 | 10 | 8 | 608 | 15 | 648 | 0.0 | 810 |
| MsG0880047563.01.T01 | AT3G25560 | 59.069 | 623 | 186 | 8 | 28 | 608 | 40 | 635 | 0.0 | 711 |
| MsG0880047563.01.T01 | AT3G25560 | 58.974 | 624 | 186 | 8 | 28 | 608 | 40 | 636 | 0.0 | 709 |
| MsG0880047563.01.T01 | AT5G16000 | 59.049 | 652 | 193 | 12 | 4 | 608 | 14 | 638 | 0.0 | 709 |
| MsG0880047563.01.T01 | AT3G25560 | 58.937 | 621 | 186 | 8 | 28 | 606 | 40 | 633 | 0.0 | 704 |
| MsG0880047563.01.T01 | AT2G23950 | 68.504 | 508 | 118 | 5 | 5 | 478 | 15 | 514 | 0.0 | 687 |
| MsG0880047563.01.T01 | AT1G60800 | 54.992 | 591 | 206 | 10 | 3 | 546 | 11 | 588 | 0.0 | 610 |
| MsG0880047563.01.T01 | AT1G60800 | 55.744 | 531 | 178 | 8 | 63 | 546 | 1 | 521 | 0.0 | 560 |
| MsG0880047563.01.T01 | AT5G45780 | 47.926 | 651 | 252 | 14 | 3 | 608 | 6 | 614 | 0.0 | 528 |
Find 172 sgRNAs with CRISPR-Local
Find 327 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTGATGTGTTTGGATT+CGG | 0.453994 | 8:-88105850 | MsG0880047563.01.T01:CDS |
| AAAAGCTGCTAATGTGCTTC+TGG | 0.309989 | 8:-88106013 | MsG0880047563.01.T01:CDS |
| AAAAGGCTGAAAGATGTAAA+TGG | 0.410708 | 8:-88106997 | MsG0880047563.01.T01:CDS |
| AAAAGGGTGCTATGCTAGAG+TGG | 0.561565 | 8:-88105765 | MsG0880047563.01.T01:CDS |
| AAACGAGAGAAGTGCGGTAA+AGG | 0.483894 | 8:+88109157 | None:intergenic |
| AAAGATGTAAATGGTAGTGC+TGG | 0.584402 | 8:-88106988 | MsG0880047563.01.T01:CDS |
| AAAGGGTGCTATGCTAGAGT+GGG | 0.619734 | 8:-88105764 | MsG0880047563.01.T01:intron |
| AACAGTGCCACGTACAGCAG+TGG | 0.627238 | 8:+88105918 | None:intergenic |
| AACGACGGTTGTGTTGGGTT+GGG | 0.480052 | 8:+88109036 | None:intergenic |
| AACTATGATAGAATAGAGGT+TGG | 0.501486 | 8:-88105048 | MsG0880047563.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACTGATGTGTTTGGATT+CGG | - | Chr8:88107899-88107918 | MsG0880047563.01.T01:intron | 30.0% | |
| AAAAGGCTGAAAGATGTAAA+TGG | - | Chr8:88106752-88106771 | MsG0880047563.01.T01:intron | 30.0% | |
| AAAATGGAGTGAAAGTGAAA+AGG | + | Chr8:88105110-88105129 | None:intergenic | 30.0% | |
| AAATTATAGCCTAATGTGGT+AGG | - | Chr8:88108314-88108333 | MsG0880047563.01.T01:intron | 30.0% | |
| AACTATGATAGAATAGAGGT+TGG | - | Chr8:88108701-88108720 | MsG0880047563.01.T01:intron | 30.0% | |
| AAGATTTGATGCACAAAAAG+AGG | + | Chr8:88105742-88105761 | None:intergenic | 30.0% | |
| AAGTGAAGGTAAAAATCAAC+TGG | + | Chr8:88107551-88107570 | None:intergenic | 30.0% | |
| ACACAATGTATTAAGCAACT+GGG | - | Chr8:88104966-88104985 | MsG0880047563.01.T01:CDS | 30.0% | |
| ACATGTCCTTGAAATGAAAT+AGG | - | Chr8:88108543-88108562 | MsG0880047563.01.T01:intron | 30.0% | |
| ACTATGAAACAAACAACCTT+TGG | - | Chr8:88105463-88105482 | MsG0880047563.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 88104404 | 88109367 | 88104404 | ID=MsG0880047563.01;Name=MsG0880047563.01 |
| Chr8 | mRNA | 88104404 | 88109367 | 88104404 | ID=MsG0880047563.01.T01;Parent=MsG0880047563.01;Name=MsG0880047563.01.T01;_AED=0.27;_eAED=0.27;_QI=340|0.77|0.9|1|1|1|10|331|608 |
| Chr8 | exon | 88104404 | 88105130 | 88104404 | ID=MsG0880047563.01.T01:exon:2249;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88105765 | 88106144 | 88105765 | ID=MsG0880047563.01.T01:exon:2248;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88106839 | 88107183 | 88106839 | ID=MsG0880047563.01.T01:exon:2247;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88107270 | 88107401 | 88107270 | ID=MsG0880047563.01.T01:exon:2246;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88107493 | 88107563 | 88107493 | ID=MsG0880047563.01.T01:exon:2245;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88107717 | 88107776 | 88107717 | ID=MsG0880047563.01.T01:exon:2244;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88108065 | 88108208 | 88108065 | ID=MsG0880047563.01.T01:exon:2243;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88108548 | 88108619 | 88108548 | ID=MsG0880047563.01.T01:exon:2242;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88108710 | 88108845 | 88108710 | ID=MsG0880047563.01.T01:exon:2241;Parent=MsG0880047563.01.T01 |
| Chr8 | exon | 88108937 | 88109367 | 88108937 | ID=MsG0880047563.01.T01:exon:2240;Parent=MsG0880047563.01.T01 |
| Chr8 | five_prime_UTR | 88109028 | 88109367 | 88109028 | ID=MsG0880047563.01.T01:five_prime_utr;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88108937 | 88109027 | 88108937 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88108710 | 88108845 | 88108710 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88108548 | 88108619 | 88108548 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88108065 | 88108208 | 88108065 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88107717 | 88107776 | 88107717 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88107493 | 88107563 | 88107493 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88107270 | 88107401 | 88107270 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88106839 | 88107183 | 88106839 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88105765 | 88106144 | 88105765 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | CDS | 88104735 | 88105130 | 88104735 | ID=MsG0880047563.01.T01:cds;Parent=MsG0880047563.01.T01 |
| Chr8 | three_prime_UTR | 88104404 | 88104734 | 88104404 | ID=MsG0880047563.01.T01:three_prime_utr;Parent=MsG0880047563.01.T01 |
| Gene Sequence |
>MsG0880047563.01.T01 ATGCCTCTCAACTTCCTTCTTCTCCTTTTCTTCTTATCATTCCTCTCCCACCAACCTCTCTCTTCCGCTTCAGAGCCTCGCAACCCCGAAGTGGTTGCTTTGATGAGTATTAAAGAAGCACTAAATGATCCACACAATGTATTAAGCAACTGGGATGAATTCTCTGTTGACCCTTGTAGTTGGGCTATGATTACTTGTTCTTCTGATTCCTCCTTTGTTATTGGCTTGGGAGCTCCTAGTCAATCTCTCTCTGGAACTTTATCTTCATCAATTGCTAATTTAACAAATCTTAAACAAGTGTTACTGCAGAATAACAATATCTCAGGCAAAATACCACCGGAACTTGGAAACCTTCCAAAGCTTCAAACTTTAGATCTTTCAAATAACAGATTCTCTGGCTTCATTCCATCTTCACTCAATCAATTGAATAGTCTTCAGTATTTGGACTTGTCTTTTAACAATCTCACTGGACCGTTGCCCAAGTTTCCAGCTAGATCATTCAACACCTCTATTGAAGGCTGCTCTGGATCAGTGACCCTTGTGCCTGTTCCTTTCTCTCAAGCTACATTACAAGGAAAACACAAGTCCAAGAAACTAGCAATTGCTCTTGGGGTTAGCTTTAGTTGTGTTTCTCTATTAGTCCTGTTTTTGGGGCTCTTTTGGTATAGAAAGAAAAGACAGCATGGAGCCATCTTGTATATTGGTGATTATAGAGAAGAGGCTGTTGTTAGCTTAGGAAATCTCATACATTTTGGTTTCAGAGAGCTCCAACACGCAACAGATAGTTTCAGCTCCAAAAATATACTCGGTGCTGGAGGTTTTGGCAATGTTTATAGGGGGAAGCTTGGAGATGGCACGTTGGTGGCTGTGAAAAGGCTGAAAGATGTAAATGGTAGTGCTGGCGAGTTACAGTTCCAAACAGAATTGGAGATGATTAGCTTGGCAGTTCACCGCAATTTGCTTCGCTTAATTGGATATTGTGCTACTCCTAATGACAAAATTCTGGTTTACCCTTACATGTCTAATGGCAGTGTGGCCTCCAGGCTTAGAGGAAAACCCGCTTTAGATTGGAACACAAGAAAGAGGATAGCAATTGGAGCTGCTCGGGGCCTTCTGTATCTTCATGAGCAATGTGATCCAAAGATCATACACAGAGATGTAAAAGCTGCTAATGTGCTTCTGGATGATGATTATGAGGCTATCGTTGGTGATTTTGGCCTTGCAAAGCTCCTTGATCATGCTGATTCCCATGTCACCACTGCTGTACGTGGCACTGTTGGACACATTGCACCGGAGTACCTCTCGACTGGCCAATCATCTGAGAAAACTGATGTGTTTGGATTCGGAATTCTCTTATTAGAGCTTATAACTGGAATGACAGCCCTTGAGTTTGGGAAAACCTTGAATCAAAAGGGTGCTATGCTAGAGTGGGTTAAAAAAATACAGCAAGAAAAGAAGGTTGAAGTTTTGGTGGACAAGGAACTAGGGAGTAACTATGATAGAATAGAGGTTGGGGAAATGCTTCAAGTAGCTTTGCTTTGCACCCAATATTTGACAGCTCACCGGCCGAAAATGTCCGAAGTGGTCCGGATGCTTGAAGGAGATGGACTTGCTGAGAAGTGGGCATCGACACATAATTATGGAAGTAACTGCTGGAGCCATAGTCACAGCAACAACAGTAGTAGCAACAGCTCATCTCGTCCTACCACTACTTCAAAACATGATGAAAATTTTCATGATCGTTCAAGCATGTTTGGCATGACCATGGATGATGATGATGATCAGTCTTTGGATTCCTATGCCATGGAACTCTCTGGCCCTAGATAA |
| Protein sequence |
>MsG0880047563.01.T01 MPLNFLLLLFFLSFLSHQPLSSASEPRNPEVVALMSIKEALNDPHNVLSNWDEFSVDPCSWAMITCSSDSSFVIGLGAPSQSLSGTLSSSIANLTNLKQVLLQNNNISGKIPPELGNLPKLQTLDLSNNRFSGFIPSSLNQLNSLQYLDLSFNNLTGPLPKFPARSFNTSIEGCSGSVTLVPVPFSQATLQGKHKSKKLAIALGVSFSCVSLLVLFLGLFWYRKKRQHGAILYIGDYREEAVVSLGNLIHFGFRELQHATDSFSSKNILGAGGFGNVYRGKLGDGTLVAVKRLKDVNGSAGELQFQTELEMISLAVHRNLLRLIGYCATPNDKILVYPYMSNGSVASRLRGKPALDWNTRKRIAIGAARGLLYLHEQCDPKIIHRDVKAANVLLDDDYEAIVGDFGLAKLLDHADSHVTTAVRGTVGHIAPEYLSTGQSSEKTDVFGFGILLLELITGMTALEFGKTLNQKGAMLEWVKKIQQEKKVEVLVDKELGSNYDRIEVGEMLQVALLCTQYLTAHRPKMSEVVRMLEGDGLAEKWASTHNYGSNCWSHSHSNNSSSNSSSRPTTTSKHDENFHDRSSMFGMTMDDDDDQSLDSYAMELSGPR* |
