AlfalfaGEDB | MsG0280008086.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008086.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008086.01.T01	XP_003594869.1	83.209	536	35	2	8	490	9	542	0	861
MsG0280008086.01.T01	XP_003594869.1	27.019	322	229	2	19	335	224	544	5.55E-31	140

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008086.01.T01	P0C7Q7	33.932	501	277	3	40	487	70	569	4.27E-102	327

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008086.01.T01	G7IND7	83.209	536	35	2	8	490	9	542	0.0	861

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0280008086.01	PPI
MsG0080048925.01	MsG0280008086.01	PPI
MsG0180001046.01	MsG0280008086.01	PPI
MsG0180001883.01	MsG0280008086.01	PPI
MsG0180001883.01	MsG0280008086.01	PPI
MsG0180001883.01	MsG0280008086.01	PPI
MsG0180003379.01	MsG0280008086.01	PPI
MsG0180003429.01	MsG0280008086.01	PPI
MsG0180003762.01	MsG0280008086.01	PPI
MsG0180005030.01	MsG0280008086.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008086.01.T01	MTR_2g035630	83.209	536	35	2	8	490	9	542	0.0	861
MsG0280008086.01.T01	MTR_2g035630	27.019	322	229	2	19	335	224	544	6.73e-35	140
MsG0280008086.01.T01	MTR_6g065540	60.862	557	148	5	1	490	1	554	0.0	659
MsG0280008086.01.T01	MTR_6g079310	64.930	499	121	5	45	490	1	498	0.0	645
MsG0280008086.01.T01	MTR_6g079310	28.440	327	233	1	39	365	170	495	7.00e-47	173
MsG0280008086.01.T01	MTR_6g078160	61.624	542	146	7	9	490	1	540	0.0	636
MsG0280008086.01.T01	MTR_6g078160	27.548	363	261	2	3	365	177	537	7.26e-46	171
MsG0280008086.01.T01	MTR_6g077540	59.571	559	145	7	10	490	12	567	0.0	635
MsG0280008086.01.T01	MTR_6g078210	62.475	501	135	2	43	490	56	556	0.0	633
MsG0280008086.01.T01	MTR_6g078210	28.857	350	214	1	57	371	210	559	8.08e-43	163

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008086.01.T01	AT1G12700	33.932	501	277	3	40	487	86	585	3.73e-103	327
MsG0280008086.01.T01	AT1G12700	25.942	451	282	2	57	472	172	605	2.67e-50	185
MsG0280008086.01.T01	AT1G12700	27.321	377	257	1	113	489	88	447	3.58e-47	176
MsG0280008086.01.T01	AT1G12700	27.184	412	272	5	40	443	224	615	1.51e-46	174
MsG0280008086.01.T01	AT1G12700	26.687	326	222	1	165	490	105	413	1.69e-39	154
MsG0280008086.01.T01	AT1G12700	27.636	275	182	1	216	490	86	343	3.78e-31	129
MsG0280008086.01.T01	AT1G12700	33.932	501	277	3	40	487	86	585	3.73e-103	327
MsG0280008086.01.T01	AT1G12700	25.942	451	282	2	57	472	172	605	2.67e-50	185
MsG0280008086.01.T01	AT1G12700	27.321	377	257	1	113	489	88	447	3.58e-47	176
MsG0280008086.01.T01	AT1G12700	27.184	412	272	5	40	443	224	615	1.51e-46	174

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Find 132 sgRNAs with CRISPR-Local

Find 362 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGGTTCGGCCTAATG+TGG	0.595625	2:-25353950	MsG0280008086.01.T01:CDS
AAAATTTGATATGACCCAAT+TGG	0.348210	2:+25354211	None:intergenic
AAACTCCATTTGTTGAGAAA+GGG	0.454255	2:+25354287	None:intergenic
AAATTTGATATGACCCAATT+GGG	0.440330	2:+25354212	None:intergenic
AACAAGGGCTTCTTGGATGA+AGG	0.457450	2:-25353059	MsG0280008086.01.T01:CDS
AACCCGTCTCAACAATTGTA+CGG	0.555892	2:+25353978	None:intergenic
AACGGGACTTGGTTAACATG+AGG	0.663880	2:-25350138	MsG0280008086.01.T01:CDS
AAGACTAGACGATGCACAAA+AGG	0.501667	2:-25353156	MsG0280008086.01.T01:CDS
AAGAGCGGTTTGGTATTGTT+TGG	0.403424	2:+25354311	None:intergenic
AAGGCCTGCTGTGAGTGTGG+AGG	0.556409	2:-25349418	MsG0280008086.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGAGAAAATGAGTTGTG+AGG	-	Chr2:25351402-25351421	MsG0280008086.01.T01:intron	30.0%
AAGATATTATACCTTTCGTC+CGG	+	Chr2:25351778-25351797	None:intergenic	30.0%
ACACTTAACCTTACCTTTAA+CGG	+	Chr2:25353590-25353609	None:intergenic	30.0%
ACAGTGAAATAGAATCATCA+AGG	+	Chr2:25349466-25349485	None:intergenic	30.0%
ACATATGTCTACATCTTTAC+AGG	+	Chr2:25353294-25353313	None:intergenic	30.0%
ACGTATACCGTTCTTATTAA+TGG	-	Chr2:25350681-25350700	MsG0280008086.01.T01:intron	30.0%
ACGTATACTTCTTTGATCAA+TGG	-	Chr2:25350786-25350805	MsG0280008086.01.T01:intron	30.0%
ACTTTCATGATAAGGTTGTA+GGG	-	Chr2:25349798-25349817	MsG0280008086.01.T01:intron	30.0%
AGCATTGTACTTTCATGATA+AGG	-	Chr2:25349790-25349809	MsG0280008086.01.T01:intron	30.0%
ATAGTAAAAGTTACAGCATC+AGG	+	Chr2:25349731-25349750	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	25349350	25354547	25349350	ID=MsG0280008086.01;Name=MsG0280008086.01
Chr2	mRNA	25349350	25354547	25349350	ID=MsG0280008086.01.T01;Parent=MsG0280008086.01;Name=MsG0280008086.01.T01;_AED=0.51;_eAED=0.51;_QI=0\|0\|0\|0.28\|1\|1\|7\|0\|664
Chr2	exon	25353338	25354547	25353338	ID=MsG0280008086.01.T01:exon:40721;Parent=MsG0280008086.01.T01
Chr2	exon	25352922	25353182	25352922	ID=MsG0280008086.01.T01:exon:40720;Parent=MsG0280008086.01.T01
Chr2	exon	25352735	25352822	25352735	ID=MsG0280008086.01.T01:exon:40719;Parent=MsG0280008086.01.T01
Chr2	exon	25352556	25352633	25352556	ID=MsG0280008086.01.T01:exon:40718;Parent=MsG0280008086.01.T01
Chr2	exon	25350302	25350393	25350302	ID=MsG0280008086.01.T01:exon:40717;Parent=MsG0280008086.01.T01
Chr2	exon	25350108	25350199	25350108	ID=MsG0280008086.01.T01:exon:40716;Parent=MsG0280008086.01.T01
Chr2	exon	25349350	25349523	25349350	ID=MsG0280008086.01.T01:exon:40715;Parent=MsG0280008086.01.T01
Chr2	CDS	25353338	25354547	25353338	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25352922	25353182	25352922	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25352735	25352822	25352735	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25352556	25352633	25352556	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25350302	25350393	25350302	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25350108	25350199	25350108	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01
Chr2	CDS	25349350	25349523	25349350	ID=MsG0280008086.01.T01:cds;Parent=MsG0280008086.01.T01

Gene Sequence

>MsG0280008086.01.T01

ATGTTTTCTTCTTCTTCTTCAAAGTTAACAAGGTATGTTGCTGGCTTTTCTGTTATAGTTCCCAAAAATTTCAATCCATCTTTTTATTTCTCTTCCTCAACTTCAAGTTCAACCCTTGATGATTCTATTTCACTGTTCAACCGCTTGCTCCATAAGAACAATCCCACCCCACCCGCCATTGAATTCGGCAAGATTTTAGGTTCCCTTCTAAAAGCCAAACAATACCAAACCGCTCTTTCCCTTTCTCAACAAATGGAGTTTAACGGAATTAAACCTGACTTTATCACTTGCAATCTCTTGATCAATTGTTTTTCCCAATTGGGTCATATCAAATTTTCATTTTCCGTATTAGCCAAGATTCTCAAAAGGGGTCATCACCCTGATGCTGTAACTTTTACTATACTCATCAAGGGTCTTTGTCTCAAAGGCGAGATTCATAAAGCATTGTACTTTCATGATAAGGTTGTAGGGCAGGGGTTTCAGTTTGATCAAGTTAGTTATGGGACGTTGATCAATGGGTTATGTAGAGTTGGAGAAACGAAAGCAGCCGTACAATTGTTGAGACGGGTTGATGGAAAATTGGTTCGGCCTAATGTGGTAATGTATAGCACGATTATTGATAGTATGTGTAAGGGTAGACTTGTTAACGATGCTTTTGATTTATATTGTGAAATGGTTGCTAAGAGAATTTCTCCTGATGTTGTTACTTATAGTTCTTTAATTAGTGGCTTATGCGTTGTTGGTAAATTGAAAGGTGCTGTTGATTTGTTTAATAGAATGATATCGGAAAACATTAATCCGAATGTGTATACTTTTAGTATATTGGTTGATGGTTTTTGTAAGGAGGGAAAAGTGAAAGAAACTAAAAATGTGTTGGCTGTGATGATGAAAAAAGACGTAAAACTTGATGTTGTTATTTATAACTCTTTAATGGATGCATATTGCTTAGTTAAACAAGTGAACAAGGCCAAGAGTTTATTCAACACTATGGCTCAAAGGGGAGTAACCCCTGACGTTTGGAGCTACAATATCATGATTAATGGATTTTGTAAGATTAAAATGGTAGATGAAGCCATGAAGCTCTTTGAAGAAATGCATTGTAAACAGATTTTTCCAAATGTGGTAACTTACAATTCCCTTGTTGATGGTTTGAGTAAAGCTTGGAGAACCTCGTGTGCTTTGGAGCTTGTCGATGAAATGCATGATAGAGTTGGAAGACTAGACGATGCACAAAAGGTTTTTGAAGATCTTTTGGTCAAAGGCTACAGTCCAAATATCTATACGTATACTTCTTTGATCAATGGATTTTGTAACAAGGGCTTCTTGGATGAAGGATTGGCCATGCTGTCAAAAATGAAAGACAATGGTTGCATTCCTAATGCTATAACTTACGAAATACTTATTCATTCTCTCTTTGAAAAAGATGAAAATGATAAAGCAGAGAAACTTCTTCGTGAAATGATAGCGAGAGTTGCTTCATCTTATGGAAAAAAAAGACACTTGTTATTCTTGTTTCTTACTTTCTTTTTTAAGTGTGTTTATCCTTCACAAACATATAGCCAATATCTTAATTCTTTGTATGAAGAAAATTTCATTTCAATACCGATTGCGACAAGGATGAATCAGTTAGTCTTTGGAGCTTTGTGGAAGAAAGGTATGGCTCGTCTGGAGAACTTCACAATGTTTGCTAAGGATAGATCATATGCTGCTTCGTTGGTCGCCGTTAAAGTTCAAGGGAATGTTGGACCTGAAGTTGACAGAGCTGTAAAACGGGACTTGGTTAACATGAGGACTTTTCATATGGAATATGACTGTCGCAGTGAGGCTCTTGAAGGAGGTGACTTAAATAACAAAGATGAAGGTCCTGATGCAACTGACACACCAACAAGCAAGCAAATGGGGAAAAGGCCTGCTGTGAGTGTGGAGGGGCTGGATTCTTCTGCTATTGAAGGGGAGAATTCTCAGAAACTAAAGAGCCAAGGATGGTCTGCTTGA

Protein sequence

>MsG0280008086.01.T01

MFSSSSSKLTRYVAGFSVIVPKNFNPSFYFSSSTSSSTLDDSISLFNRLLHKNNPTPPAIEFGKILGSLLKAKQYQTALSLSQQMEFNGIKPDFITCNLLINCFSQLGHIKFSFSVLAKILKRGHHPDAVTFTILIKGLCLKGEIHKALYFHDKVVGQGFQFDQVSYGTLINGLCRVGETKAAVQLLRRVDGKLVRPNVVMYSTIIDSMCKGRLVNDAFDLYCEMVAKRISPDVVTYSSLISGLCVVGKLKGAVDLFNRMISENINPNVYTFSILVDGFCKEGKVKETKNVLAVMMKKDVKLDVVIYNSLMDAYCLVKQVNKAKSLFNTMAQRGVTPDVWSYNIMINGFCKIKMVDEAMKLFEEMHCKQIFPNVVTYNSLVDGLSKAWRTSCALELVDEMHDRVGRLDDAQKVFEDLLVKGYSPNIYTYTSLINGFCNKGFLDEGLAMLSKMKDNGCIPNAITYEILIHSLFEKDENDKAEKLLREMIARVASSYGKKRHLLFLFLTFFFKCVYPSQTYSQYLNSLYEENFISIPIATRMNQLVFGALWKKGMARLENFTMFAKDRSYAASLVAVKVQGNVGPEVDRAVKRDLVNMRTFHMEYDCRSEALEGGDLNNKDEGPDATDTPTSKQMGKRPAVSVEGLDSSAIEGENSQKLKSQGWSA*