AlfalfaGEDB | Msa0001330

M. sativa cultivar zhongmu-4 / Msa0001330

KEGG: K09522
GO: GO:0001558 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0030308 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0040008 GO:0042393 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045926 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061649 GO:0065007 GO:0080090 GO:0140030 GO:0140035 GO:0140036 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF00226.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0001330	sp\|Q7TQ20\|DNJC2_RAT	35.294	595	284	16	92	653	83	609	2.71e-89	293

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0001330	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001330	Msa0523050	0.900192	9.368675e-78	-8.615850e-47
Msa0001330	Msa0656810	0.802188	6.314763e-49	-8.615850e-47
Msa0001330	Msa0712560	0.815726	7.974064e-52	-8.615850e-47
Msa0001330	Msa0791090	0.821721	3.472513e-53	-8.615850e-47
Msa0001330	Msa1383740	0.906226	1.860621e-80	-8.615850e-47
Msa0001330	Msa1409260	0.800211	1.602957e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0001330	MtrunA17_Chr3g0142871	97.706	654	14	1	1	654	1	653	0.0	1310
Msa0001330	MtrunA17_Chr7g0269901	97.095	654	18	1	1	654	1	653	0.0	1306
Msa0001330	MtrunA17_Chr3g0142891	83.712	264	35	2	393	654	1	258	5.20e-145	421
Msa0001330	MtrunA17_Chr8g0383991	29.439	214	95	3	443	653	140	300	8.01e-21	93.6
Msa0001330	MtrunA17_Chr4g0061421	30.417	240	115	9	98	300	9	233	2.30e-17	86.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0001330	AT3G11450.1	63.939	660	210	11	4	654	7	647	0.0	734
Msa0001330	AT5G06110.1	59.492	669	239	10	2	651	5	660	0.0	713
Msa0001330	AT5G06110.2	59.492	669	239	10	2	651	5	660	0.0	713
Msa0001330	AT5G45420.2	31.959	194	87	3	459	652	158	306	7.81e-19	88.2
Msa0001330	AT5G45420.1	31.959	194	87	3	459	652	158	306	7.81e-19	88.2
Msa0001330	AT5G45420.3	31.959	194	87	3	459	652	158	306	7.81e-19	88.2
Msa0001330	AT1G74250.1	29.167	240	117	6	98	297	12	238	4.85e-16	82.4

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Find 159 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAAGCTAACCAGAGAT+GGG	0.594916	1_1:+3021804	Msa0001330:CDS
AACACCTTGTAGAAGTCTTG+TGG	0.578361	1_1:-3020533	None:intergenic
AACTAGAGAAGAATTGGAAG+GGG	0.608691	1_1:+3021573	Msa0001330:CDS
AACTTCCTTGAGGTTTCATC+CGG	0.430850	1_1:+3020328	Msa0001330:CDS
AAGAAATGTATCAAAAGCTT+TGG	0.451091	1_1:-3021506	None:intergenic
AAGATCAAACTCATCAGCCT+GGG	0.518267	1_1:-3020693	None:intergenic
AAGCATTATCAACAAGAGTG+CGG	0.686922	1_1:-3020808	None:intergenic
AAGCTGGAGAGAGTTTCCCC+AGG	0.640812	1_1:+3020676	Msa0001330:CDS
AAGTCGTAAAATCTAAAAGC+AGG	0.552023	1_1:-3021966	None:intergenic
AATACTGAGAAGAAAGGAAG+AGG	0.562192	1_1:+3020847	Msa0001330:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAAGTGAAATAAGAAGCT+CGG	-	chr1_1:3019715-3019734	None:intergenic	30.0%
CTTGTGAGTAAGTAATAAGA+CGG	-	chr1_1:3019986-3020005	None:intergenic	30.0%
TGTTTCATCTAATTGTCTTC+CGG	+	chr1_1:3020031-3020050	Msa0001330:CDS	30.0%
GCAAGATCATTATGCATTAT+TGG	+	chr1_1:3020247-3020266	Msa0001330:CDS	30.0%
GAAAAAGGAGTCCAAATATA+TGG	+	chr1_1:3020889-3020908	Msa0001330:CDS	30.0%
CAACTTCTTTGCCATATATT+TGG	-	chr1_1:3020903-3020922	None:intergenic	30.0%
CAAATATATGGCAAAGAAGT+TGG	+	chr1_1:3020901-3020920	Msa0001330:CDS	30.0%
CATTTGATATCGAACAATTG+AGG	+	chr1_1:3021143-3021162	Msa0001330:CDS	30.0%
TTTGATATCGAACAATTGAG+GGG	+	chr1_1:3021145-3021164	Msa0001330:CDS	30.0%
TTGATACATTTCTTGAGAAG+AGG	+	chr1_1:3021515-3021534	Msa0001330:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	3019216	3022287	3019216	ID=Msa0001330;Name=Msa0001330
chr1_1	mRNA	3019216	3022287	3019216	ID=Msa0001330-mRNA-1;Parent=Msa0001330;Name=Msa0001330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=264\|1\|1\|1\|0\|1\|2\|360\|654
chr1_1	exon	3019216	3019410	3019216	ID=Msa0001330-mRNA-1:exon:668;Parent=Msa0001330-mRNA-1
chr1_1	exon	3019894	3022287	3019894	ID=Msa0001330-mRNA-1:exon:669;Parent=Msa0001330-mRNA-1
chr1_1	five_prime_UTR	3019216	3019410	3019216	ID=Msa0001330-mRNA-1:five_prime_utr;Parent=Msa0001330-mRNA-1
chr1_1	five_prime_UTR	3019894	3019962	3019894	ID=Msa0001330-mRNA-1:five_prime_utr;Parent=Msa0001330-mRNA-1
chr1_1	CDS	3019963	3021927	3019963	ID=Msa0001330-mRNA-1:cds;Parent=Msa0001330-mRNA-1
chr1_1	three_prime_UTR	3021928	3022287	3021928	ID=Msa0001330-mRNA-1:three_prime_utr;Parent=Msa0001330-mRNA-1

Gene Sequence

>Msa0001330

ATGGCTGTTCAAACAAAATACCGTCTTATTACTTACTCACAAGAGCTTGTAGATGGGCAGCCTATTTTTGTTTCATCTAATTGTCTTCCGGTTAAGGCGTTGAAATATGACCCGGCTGGTCACTCTTTTCATGCTGCTGCACTTAAACTTCTCGGTGTTACAAAAGAAGATAACAAAGATGCTGATAAGAAGAATGTGGTTGAAGATAAGGAACATGTTTATTTGCCTTCTGATTCTTACAGCAGCAAGAGCAAGAAGAAATCTGGTGACGGAGACAAGCAACAGCAAGATCATTATGCATTATTGGGTTTGAGCCATCTTAGATATCTTGCTACGGAGGATCAAATTCGTAAAAGCTATCATAAAACTTCCTTGAGGTTTCATCCGGACAAACAGGCTGCTGCTGTTCTTTCCGAGCAAACTGAAGCTGCGAAGGAGGCAAAGAAGATTGAAATAGATGTTCATTTTAAGGCAATCCAAGAGGCTTATGCAGTGTTGGTCGATCCCGTAAAAAGAAGAATTTATGATTCTACAGACGAGTTTGATGATGAGATTCCCACTGACTGTGATCCACAAGACTTCTACAAGGTGTTTGGTCCTGCTTTTATGAGGAATGGACGGTGGTCGGTTAATCAACCAATTCCATCTCTAGGTGATGATAAGTCTTCAATAAAGGAAGTTGATAGTTTCTACAATTTTTGGTATTCCTTTAAAAGCTGGAGAGAGTTTCCCCAGGCTGATGAGTTTGATCTTGAGCAAGCTGAATCCCGAGATCATAAAAGGTGGATGGAAAGGCAAAATGCAAAACTGTCAGAAAAAGCTAGGAAGGAAGAGTATGCACGTATCCGCACTCTTGTTGATAATGCTTATAAGAGGGACCCTAGAATACTGAGAAGAAAGGAAGAGGCAAAAGCTGAGAAGAAAAGGAAAAAGGAGTCCAAATATATGGCAAAGAAGTTGGAGGAGGAAGAAGCGGCCAGAATTGCTGAAGAGGAGAAACAACGAAAAGCAGAGGAAGATAAAAAAGCTGCCGAAGCTGCTTTGCAACAGAAGAAGGTGAAAGAGAAGGAGAAAAAGCTCTTGCGGAAGGAGCGAACACGTCTTCGAACTCTCTCAAGACCTATCTTGTCACAACACATACTTGATATTGCTGAAGACGATGTAGAAGAGCTTTGCATGTCATTTGATATCGAACAATTGAGGGGTTTGTGTGAGAAAATCGAAGGCAAAGAGGTGTTAGAGCAAGCAGAAGCTCTAAAAGATGCACTGAGTTGCAAGAAAAACGTGGTTGATGAGAAAAGTAATCAACAAAATGGCTATGTCAAGGCTAATGGAAGCAGTAGTTCTCTAGCAGGCTACACAGAGAAGAAGGAGAAACCTTGGACTAAAGAAGAGATTGAGCTATTGAGAAAAGGAATACAAAAGTTTCCCAAAGGAACTTCAAGGAGATGGGAGGTTGTTTCAGAATACATTGGCACTGGAAGATCTGTTGAAGAAATAATGAAAGCAACTAAAACCGTTCTCCTCCAGAAGCCCGATACAGCCAAAGCTTTTGATACATTTCTTGAGAAGAGGAAACCTGCAGCACAATCAATTGCATCCCCATTGTCAACTAGAGAAGAATTGGAAGGGGTCTCAATACCAGCAACAACAACTGAAAATAGTGATGCAAAAACAACAACTACTATACCAACACCGACGATGACGACGACAACAATTCCAACACCGGTACCGACAGCAACAAGCAGCATCAACTCGGAAGACTCTCAAGGAGTTTCAGAACAAGAGGCGTGGTCTGCAGTGCAGGAAAGAGCATTAGTTCAAGCTTTAAAAACCTTCCCAAAGGAAGCTAACCAGAGATGGGAACGAGTAGCTGCAGCTGTACCTGGGAAGACCGTGATCCAGTGCAAGAAAAAATTTGCCATGATGAAGGAAAATTTCAGGAACAAGAAAACTGCAGTTTGA

Protein sequence

>Msa0001330

MAVQTKYRLITYSQELVDGQPIFVSSNCLPVKALKYDPAGHSFHAAALKLLGVTKEDNKDADKKNVVEDKEHVYLPSDSYSSKSKKKSGDGDKQQQDHYALLGLSHLRYLATEDQIRKSYHKTSLRFHPDKQAAAVLSEQTEAAKEAKKIEIDVHFKAIQEAYAVLVDPVKRRIYDSTDEFDDEIPTDCDPQDFYKVFGPAFMRNGRWSVNQPIPSLGDDKSSIKEVDSFYNFWYSFKSWREFPQADEFDLEQAESRDHKRWMERQNAKLSEKARKEEYARIRTLVDNAYKRDPRILRRKEEAKAEKKRKKESKYMAKKLEEEEAARIAEEEKQRKAEEDKKAAEAALQQKKVKEKEKKLLRKERTRLRTLSRPILSQHILDIAEDDVEELCMSFDIEQLRGLCEKIEGKEVLEQAEALKDALSCKKNVVDEKSNQQNGYVKANGSSSSLAGYTEKKEKPWTKEEIELLRKGIQKFPKGTSRRWEVVSEYIGTGRSVEEIMKATKTVLLQKPDTAKAFDTFLEKRKPAAQSIASPLSTREELEGVSIPATTTENSDAKTTTTIPTPTMTTTTIPTPVPTATSSINSEDSQGVSEQEAWSAVQERALVQALKTFPKEANQRWERVAAAVPGKTVIQCKKKFAMMKENFRNKKTAV*