Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000200 | Msa0006610 | 0.937230 | 4.852848e-98 | -8.615850e-47 |
| Msa0006610 | Msa0033630 | 0.848311 | 6.645065e-60 | -8.615850e-47 |
| Msa0006610 | Msa1024130 | 0.853836 | 1.835687e-61 | -8.615850e-47 |
| Msa0006610 | Msa1107960 | 0.871232 | 7.991392e-67 | -8.615850e-47 |
| Msa0006610 | Msa1140180 | 0.915666 | 4.489918e-85 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0006610 | MtrunA17_Chr4g0023901 | 96.364 | 55 | 2 | 0 | 1 | 55 | 1 | 55 | 1.09e-32 | 107 |
| Msa0006610 | MtrunA17_Chr2g0281741 | 64.835 | 91 | 16 | 3 | 1 | 84 | 1 | 82 | 3.98e-29 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGTAGCCATAACTGTAAT+AGG | 0.226353 | 1_1:-9562278 | None:intergenic |
| TTGTTTGTTTGGTGGAAGTT+GGG | 0.310395 | 1_1:-9562070 | None:intergenic |
| CCTCACCCCGACCGTCCTCC+TGG | 0.346667 | 1_1:+9562232 | Msa0006610:CDS |
| ATTGTTTGTTTGGTGGAAGT+TGG | 0.347178 | 1_1:-9562071 | None:intergenic |
| TTCGGTCATGATTGTTTGTT+TGG | 0.366531 | 1_1:-9562081 | None:intergenic |
| CCAGGAGGACGGTCGGGGTG+AGG | 0.369145 | 1_1:-9562232 | None:intergenic |
| TAAGGGTTAGGGTGAAGAGG+TGG | 0.375340 | 1_1:-9562192 | None:intergenic |
| TCGGATCCAGGAGGACGGTC+GGG | 0.380838 | 1_1:-9562238 | None:intergenic |
| GGGGCGGAGGGGTCGGATCC+AGG | 0.386026 | 1_1:-9562250 | None:intergenic |
| ACCGATCTGGAGTGAATTCA+TGG | 0.400497 | 1_1:-9562020 | None:intergenic |
| GGATTTGAGTTTGCCGTGTT+CGG | 0.412366 | 1_1:-9562099 | None:intergenic |
| GGTGAGGGAAACCGATGGCG+GGG | 0.413789 | 1_1:-9562216 | None:intergenic |
| GATTGCGGCGACGGCGGAGG+AGG | 0.416666 | 1_1:-9562134 | None:intergenic |
| AGGGTGAAGAGGTGGGAAAT+TGG | 0.418104 | 1_1:-9562184 | None:intergenic |
| GGGTGAGGGAAACCGATGGC+GGG | 0.424253 | 1_1:-9562217 | None:intergenic |
| TGTTTGTTTGGTGGAAGTTG+GGG | 0.425035 | 1_1:-9562069 | None:intergenic |
| CGGATCCAGGAGGACGGTCG+GGG | 0.433438 | 1_1:-9562237 | None:intergenic |
| GTAATAGGGAGGAGGGGCGG+AGG | 0.435218 | 1_1:-9562263 | None:intergenic |
| CCTCCTCCCTATTACAGTTA+TGG | 0.441098 | 1_1:+9562271 | Msa0006610:CDS |
| AACGAAGCCACTCACCGATC+TGG | 0.446314 | 1_1:-9562033 | None:intergenic |
| GGGGTGAGGGAAACCGATGG+CGG | 0.446566 | 1_1:-9562218 | None:intergenic |
| GGGTAAGGGTTAGGGTGAAG+AGG | 0.448412 | 1_1:-9562195 | None:intergenic |
| GGGAGGAGGGGCGGAGGGGT+CGG | 0.470296 | 1_1:-9562257 | None:intergenic |
| TAGTAGCCATAACTGTAATA+GGG | 0.470585 | 1_1:-9562277 | None:intergenic |
| GTGAGGGAAACCGATGGCGG+GGG | 0.471021 | 1_1:-9562215 | None:intergenic |
| CAGGAGGACGGTCGGGGTGA+GGG | 0.471785 | 1_1:-9562231 | None:intergenic |
| CGGAGGAGGGTGGCCGAAGT+AGG | 0.477759 | 1_1:-9562120 | None:intergenic |
| AATAGGGAGGAGGGGCGGAG+GGG | 0.479253 | 1_1:-9562261 | None:intergenic |
| GGTTGAAAGGATTGCGGCGA+CGG | 0.493819 | 1_1:-9562143 | None:intergenic |
| GTCGGATCCAGGAGGACGGT+CGG | 0.498258 | 1_1:-9562239 | None:intergenic |
| TAATAGGGAGGAGGGGCGGA+GGG | 0.504583 | 1_1:-9562262 | None:intergenic |
| AAGGATTGCGGCGACGGCGG+AGG | 0.507727 | 1_1:-9562137 | None:intergenic |
| AAGGGTTAGGGTGAAGAGGT+GGG | 0.523980 | 1_1:-9562191 | None:intergenic |
| GCGGCGACGGCGGAGGAGGG+TGG | 0.525558 | 1_1:-9562130 | None:intergenic |
| GTTTGTTTGGTGGAAGTTGG+GGG | 0.531733 | 1_1:-9562068 | None:intergenic |
| GGTCATGATTGTTTGTTTGG+TGG | 0.539968 | 1_1:-9562078 | None:intergenic |
| CATAACTGTAATAGGGAGGA+GGG | 0.545322 | 1_1:-9562270 | None:intergenic |
| CTCGTACTGAGGAGGTTGAA+AGG | 0.548056 | 1_1:-9562156 | None:intergenic |
| ATTGCGGCGACGGCGGAGGA+GGG | 0.550016 | 1_1:-9562133 | None:intergenic |
| CCATAACTGTAATAGGGAGG+AGG | 0.554457 | 1_1:-9562271 | None:intergenic |
| CAAACAATCATGACCGAACA+CGG | 0.568465 | 1_1:+9562086 | Msa0006610:CDS |
| GGCAAACTCAAATCCTACTT+CGG | 0.572114 | 1_1:+9562107 | Msa0006610:CDS |
| GCGGAGGGGTCGGATCCAGG+AGG | 0.575604 | 1_1:-9562247 | None:intergenic |
| TGAAAGGATTGCGGCGACGG+CGG | 0.584093 | 1_1:-9562140 | None:intergenic |
| AGGGGTCGGATCCAGGAGGA+CGG | 0.585435 | 1_1:-9562243 | None:intergenic |
| AATTGGAAAGACTCGTACTG+AGG | 0.630700 | 1_1:-9562167 | None:intergenic |
| TCCATGAATTCACTCCAGAT+CGG | 0.641820 | 1_1:+9562019 | None:intergenic |
| GTCGGGGTGAGGGAAACCGA+TGG | 0.644761 | 1_1:-9562221 | None:intergenic |
| ACTGTAATAGGGAGGAGGGG+CGG | 0.647217 | 1_1:-9562266 | None:intergenic |
| TGGAAAGACTCGTACTGAGG+AGG | 0.658466 | 1_1:-9562164 | None:intergenic |
| TGAGGAGGTTGAAAGGATTG+CGG | 0.659256 | 1_1:-9562149 | None:intergenic |
| TAGCCATAACTGTAATAGGG+AGG | 0.673963 | 1_1:-9562274 | None:intergenic |
| ATTCACTCCAGATCGGTGAG+TGG | 0.692086 | 1_1:+9562026 | Msa0006610:CDS |
| ATAACTGTAATAGGGAGGAG+GGG | 0.695524 | 1_1:-9562269 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGTAGCCATAACTGTAATA+GGG | - | chr1_1:9562280-9562299 | None:intergenic | 30.0% | |
| TTAGTAGCCATAACTGTAAT+AGG | - | chr1_1:9562281-9562300 | None:intergenic | 30.0% | |
| ! | TTGTTTGTTTGGTGGAAGTT+GGG | - | chr1_1:9562073-9562092 | None:intergenic | 35.0% |
| ! | ATTGTTTGTTTGGTGGAAGT+TGG | - | chr1_1:9562074-9562093 | None:intergenic | 35.0% |
| TTCGGTCATGATTGTTTGTT+TGG | - | chr1_1:9562084-9562103 | None:intergenic | 35.0% | |
| ! | TGTTTGTTTGGTGGAAGTTG+GGG | - | chr1_1:9562072-9562091 | None:intergenic | 40.0% |
| GGTCATGATTGTTTGTTTGG+TGG | - | chr1_1:9562081-9562100 | None:intergenic | 40.0% | |
| CAAACAATCATGACCGAACA+CGG | + | chr1_1:9562086-9562105 | Msa0006610:CDS | 40.0% | |
| GGCAAACTCAAATCCTACTT+CGG | + | chr1_1:9562107-9562126 | Msa0006610:CDS | 40.0% | |
| AATTGGAAAGACTCGTACTG+AGG | - | chr1_1:9562170-9562189 | None:intergenic | 40.0% | |
| ATAACTGTAATAGGGAGGAG+GGG | - | chr1_1:9562272-9562291 | None:intergenic | 40.0% | |
| CATAACTGTAATAGGGAGGA+GGG | - | chr1_1:9562273-9562292 | None:intergenic | 40.0% | |
| TAGCCATAACTGTAATAGGG+AGG | - | chr1_1:9562277-9562296 | None:intergenic | 40.0% | |
| ! | GTTTGTTTGGTGGAAGTTGG+GGG | - | chr1_1:9562071-9562090 | None:intergenic | 45.0% |
| ! | GGATTTGAGTTTGCCGTGTT+CGG | - | chr1_1:9562102-9562121 | None:intergenic | 45.0% |
| TGAGGAGGTTGAAAGGATTG+CGG | - | chr1_1:9562152-9562171 | None:intergenic | 45.0% | |
| CCATAACTGTAATAGGGAGG+AGG | - | chr1_1:9562274-9562293 | None:intergenic | 45.0% | |
| CCTCCTCCCTATTACAGTTA+TGG | + | chr1_1:9562271-9562290 | Msa0006610:CDS | 45.0% | |
| ATTCACTCCAGATCGGTGAG+TGG | + | chr1_1:9562026-9562045 | Msa0006610:CDS | 50.0% | |
| TTTGGTGGAAGTTGGGGGTT+AGG | - | chr1_1:9562066-9562085 | None:intergenic | 50.0% | |
| CTCGTACTGAGGAGGTTGAA+AGG | - | chr1_1:9562159-9562178 | None:intergenic | 50.0% | |
| TGGAAAGACTCGTACTGAGG+AGG | - | chr1_1:9562167-9562186 | None:intergenic | 50.0% | |
| AGGGTGAAGAGGTGGGAAAT+TGG | - | chr1_1:9562187-9562206 | None:intergenic | 50.0% | |
| AAGGGTTAGGGTGAAGAGGT+GGG | - | chr1_1:9562194-9562213 | None:intergenic | 50.0% | |
| TAAGGGTTAGGGTGAAGAGG+TGG | - | chr1_1:9562195-9562214 | None:intergenic | 50.0% | |
| AACGAAGCCACTCACCGATC+TGG | - | chr1_1:9562036-9562055 | None:intergenic | 55.0% | |
| GTGGAAGTTGGGGGTTAGGA+GGG | - | chr1_1:9562062-9562081 | None:intergenic | 55.0% | |
| GGTTGAAAGGATTGCGGCGA+CGG | - | chr1_1:9562146-9562165 | None:intergenic | 55.0% | |
| GGGTAAGGGTTAGGGTGAAG+AGG | - | chr1_1:9562198-9562217 | None:intergenic | 55.0% | |
| ACTGTAATAGGGAGGAGGGG+CGG | - | chr1_1:9562269-9562288 | None:intergenic | 55.0% | |
| GGTGGAAGTTGGGGGTTAGG+AGG | - | chr1_1:9562063-9562082 | None:intergenic | 60.0% | |
| TGAAAGGATTGCGGCGACGG+CGG | - | chr1_1:9562143-9562162 | None:intergenic | 60.0% | |
| !! | GATGGCGGGGGTAAGGGTTA+GGG | - | chr1_1:9562206-9562225 | None:intergenic | 60.0% |
| CTAACCCTTACCCCCGCCAT+CGG | + | chr1_1:9562205-9562224 | Msa0006610:CDS | 60.0% | |
| !! | GAAACCGATGGCGGGGGTAA+GGG | - | chr1_1:9562212-9562231 | None:intergenic | 60.0% |
| TAATAGGGAGGAGGGGCGGA+GGG | - | chr1_1:9562265-9562284 | None:intergenic | 60.0% | |
| !! | CGATGGCGGGGGTAAGGGTT+AGG | - | chr1_1:9562207-9562226 | None:intergenic | 65.0% |
| !! | GGAAACCGATGGCGGGGGTA+AGG | - | chr1_1:9562213-9562232 | None:intergenic | 65.0% |
| ! | GTGAGGGAAACCGATGGCGG+GGG | - | chr1_1:9562218-9562237 | None:intergenic | 65.0% |
| GGTGAGGGAAACCGATGGCG+GGG | - | chr1_1:9562219-9562238 | None:intergenic | 65.0% | |
| GGGTGAGGGAAACCGATGGC+GGG | - | chr1_1:9562220-9562239 | None:intergenic | 65.0% | |
| GGGGTGAGGGAAACCGATGG+CGG | - | chr1_1:9562221-9562240 | None:intergenic | 65.0% | |
| GTCGGGGTGAGGGAAACCGA+TGG | - | chr1_1:9562224-9562243 | None:intergenic | 65.0% | |
| TCGGATCCAGGAGGACGGTC+GGG | - | chr1_1:9562241-9562260 | None:intergenic | 65.0% | |
| GTCGGATCCAGGAGGACGGT+CGG | - | chr1_1:9562242-9562261 | None:intergenic | 65.0% | |
| AGGGGTCGGATCCAGGAGGA+CGG | - | chr1_1:9562246-9562265 | None:intergenic | 65.0% | |
| AATAGGGAGGAGGGGCGGAG+GGG | - | chr1_1:9562264-9562283 | None:intergenic | 65.0% | |
| GTAATAGGGAGGAGGGGCGG+AGG | - | chr1_1:9562266-9562285 | None:intergenic | 65.0% | |
| CGGAGGAGGGTGGCCGAAGT+AGG | - | chr1_1:9562123-9562142 | None:intergenic | 70.0% | |
| ATTGCGGCGACGGCGGAGGA+GGG | - | chr1_1:9562136-9562155 | None:intergenic | 70.0% | |
| AAGGATTGCGGCGACGGCGG+AGG | - | chr1_1:9562140-9562159 | None:intergenic | 70.0% | |
| CAGGAGGACGGTCGGGGTGA+GGG | - | chr1_1:9562234-9562253 | None:intergenic | 70.0% | |
| CGGATCCAGGAGGACGGTCG+GGG | - | chr1_1:9562240-9562259 | None:intergenic | 70.0% | |
| GATTGCGGCGACGGCGGAGG+AGG | - | chr1_1:9562137-9562156 | None:intergenic | 75.0% | |
| CCAGGAGGACGGTCGGGGTG+AGG | - | chr1_1:9562235-9562254 | None:intergenic | 75.0% | |
| CCTCACCCCGACCGTCCTCC+TGG | + | chr1_1:9562232-9562251 | Msa0006610:CDS | 75.0% | |
| GCGGAGGGGTCGGATCCAGG+AGG | - | chr1_1:9562250-9562269 | None:intergenic | 75.0% | |
| ! | GGGGCGGAGGGGTCGGATCC+AGG | - | chr1_1:9562253-9562272 | None:intergenic | 80.0% |
| ! | GGGAGGAGGGGCGGAGGGGT+CGG | - | chr1_1:9562260-9562279 | None:intergenic | 80.0% |
| ! | GCGGCGACGGCGGAGGAGGG+TGG | - | chr1_1:9562133-9562152 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 9562022 | 9562300 | 9562022 | ID=Msa0006610;Name=Msa0006610 |
| chr1_1 | mRNA | 9562022 | 9562300 | 9562022 | ID=Msa0006610-mRNA-1;Parent=Msa0006610;Name=Msa0006610-mRNA-1;_AED=0.45;_eAED=0.53;_QI=0|-1|0|1|-1|1|1|0|92 |
| chr1_1 | exon | 9562022 | 9562300 | 9562022 | ID=Msa0006610-mRNA-1:exon:3410;Parent=Msa0006610-mRNA-1 |
| chr1_1 | CDS | 9562022 | 9562300 | 9562022 | ID=Msa0006610-mRNA-1:cds;Parent=Msa0006610-mRNA-1 |
| Gene Sequence |
| Protein sequence |
