Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000200 | Msa0033630 | 0.842537 | 2.433857e-58 | -8.615850e-47 |
| Msa0006610 | Msa0033630 | 0.848311 | 6.645065e-60 | -8.615850e-47 |
| Msa0022690 | Msa0033630 | 0.888763 | 4.422804e-73 | -8.615850e-47 |
| Msa0033630 | Msa1024130 | 0.902734 | 7.160131e-79 | -8.615850e-47 |
| Msa0033630 | Msa1107960 | 0.913036 | 9.794427e-84 | -8.615850e-47 |
| Msa0033630 | Msa1140180 | 0.802449 | 5.580124e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0033630 | MtrunA17_Chr4g0023901 | 96.364 | 55 | 2 | 0 | 1 | 55 | 1 | 55 | 1.09e-32 | 107 |
| Msa0033630 | MtrunA17_Chr2g0281741 | 64.835 | 91 | 16 | 3 | 1 | 84 | 1 | 82 | 3.98e-29 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGTAGCCATAACTGTAAT+AGG | 0.226353 | 1_1:-61230505 | None:intergenic |
| TTGTTTGTTTGGTGGAAGTT+GGG | 0.310395 | 1_1:-61230297 | None:intergenic |
| CCTCACCCCGACCGTCCTCC+TGG | 0.346667 | 1_1:+61230459 | Msa0033630:CDS |
| ATTGTTTGTTTGGTGGAAGT+TGG | 0.347178 | 1_1:-61230298 | None:intergenic |
| TTCGGTCATGATTGTTTGTT+TGG | 0.366531 | 1_1:-61230308 | None:intergenic |
| CCAGGAGGACGGTCGGGGTG+AGG | 0.369145 | 1_1:-61230459 | None:intergenic |
| TAAGGGTTAGGGTGAAGAGG+TGG | 0.375340 | 1_1:-61230419 | None:intergenic |
| TCGGATCCAGGAGGACGGTC+GGG | 0.380838 | 1_1:-61230465 | None:intergenic |
| GGGGCGGAGGGGTCGGATCC+AGG | 0.386026 | 1_1:-61230477 | None:intergenic |
| ACCGATCTGGAGTGAATTCA+TGG | 0.400497 | 1_1:-61230247 | None:intergenic |
| GGATTTGAGTTTGCCGTGTT+CGG | 0.412366 | 1_1:-61230326 | None:intergenic |
| GGTGAGGGAAACCGATGGCG+GGG | 0.413789 | 1_1:-61230443 | None:intergenic |
| GATTGCGGCGACGGCGGAGG+AGG | 0.416666 | 1_1:-61230361 | None:intergenic |
| AGGGTGAAGAGGTGGGAAAT+TGG | 0.418104 | 1_1:-61230411 | None:intergenic |
| GGGTGAGGGAAACCGATGGC+GGG | 0.424253 | 1_1:-61230444 | None:intergenic |
| TGTTTGTTTGGTGGAAGTTG+GGG | 0.425035 | 1_1:-61230296 | None:intergenic |
| CGGATCCAGGAGGACGGTCG+GGG | 0.433438 | 1_1:-61230464 | None:intergenic |
| GTAATAGGGAGGAGGGGCGG+AGG | 0.435218 | 1_1:-61230490 | None:intergenic |
| CCTCCTCCCTATTACAGTTA+TGG | 0.441098 | 1_1:+61230498 | Msa0033630:CDS |
| AACGAAGCCACTCACCGATC+TGG | 0.446314 | 1_1:-61230260 | None:intergenic |
| GGGGTGAGGGAAACCGATGG+CGG | 0.446566 | 1_1:-61230445 | None:intergenic |
| GGGTAAGGGTTAGGGTGAAG+AGG | 0.448412 | 1_1:-61230422 | None:intergenic |
| GGGAGGAGGGGCGGAGGGGT+CGG | 0.470296 | 1_1:-61230484 | None:intergenic |
| TAGTAGCCATAACTGTAATA+GGG | 0.470585 | 1_1:-61230504 | None:intergenic |
| GTGAGGGAAACCGATGGCGG+GGG | 0.471021 | 1_1:-61230442 | None:intergenic |
| CAGGAGGACGGTCGGGGTGA+GGG | 0.471785 | 1_1:-61230458 | None:intergenic |
| CGGAGGAGGGTGGCCGAAGT+AGG | 0.477759 | 1_1:-61230347 | None:intergenic |
| AATAGGGAGGAGGGGCGGAG+GGG | 0.479253 | 1_1:-61230488 | None:intergenic |
| GGTTGAAAGGATTGCGGCGA+CGG | 0.493819 | 1_1:-61230370 | None:intergenic |
| GTCGGATCCAGGAGGACGGT+CGG | 0.498258 | 1_1:-61230466 | None:intergenic |
| TAATAGGGAGGAGGGGCGGA+GGG | 0.504583 | 1_1:-61230489 | None:intergenic |
| AAGGATTGCGGCGACGGCGG+AGG | 0.507727 | 1_1:-61230364 | None:intergenic |
| AAGGGTTAGGGTGAAGAGGT+GGG | 0.523980 | 1_1:-61230418 | None:intergenic |
| GCGGCGACGGCGGAGGAGGG+TGG | 0.525558 | 1_1:-61230357 | None:intergenic |
| GTTTGTTTGGTGGAAGTTGG+GGG | 0.531733 | 1_1:-61230295 | None:intergenic |
| GGTCATGATTGTTTGTTTGG+TGG | 0.539968 | 1_1:-61230305 | None:intergenic |
| CATAACTGTAATAGGGAGGA+GGG | 0.545322 | 1_1:-61230497 | None:intergenic |
| CTCGTACTGAGGAGGTTGAA+AGG | 0.548056 | 1_1:-61230383 | None:intergenic |
| ATTGCGGCGACGGCGGAGGA+GGG | 0.550016 | 1_1:-61230360 | None:intergenic |
| CCATAACTGTAATAGGGAGG+AGG | 0.554457 | 1_1:-61230498 | None:intergenic |
| CAAACAATCATGACCGAACA+CGG | 0.568465 | 1_1:+61230313 | Msa0033630:CDS |
| GGCAAACTCAAATCCTACTT+CGG | 0.572114 | 1_1:+61230334 | Msa0033630:CDS |
| GCGGAGGGGTCGGATCCAGG+AGG | 0.575604 | 1_1:-61230474 | None:intergenic |
| TGAAAGGATTGCGGCGACGG+CGG | 0.584093 | 1_1:-61230367 | None:intergenic |
| AGGGGTCGGATCCAGGAGGA+CGG | 0.585435 | 1_1:-61230470 | None:intergenic |
| AATTGGAAAGACTCGTACTG+AGG | 0.630700 | 1_1:-61230394 | None:intergenic |
| TCCATGAATTCACTCCAGAT+CGG | 0.641820 | 1_1:+61230246 | None:intergenic |
| GTCGGGGTGAGGGAAACCGA+TGG | 0.644761 | 1_1:-61230448 | None:intergenic |
| ACTGTAATAGGGAGGAGGGG+CGG | 0.647217 | 1_1:-61230493 | None:intergenic |
| TGGAAAGACTCGTACTGAGG+AGG | 0.658466 | 1_1:-61230391 | None:intergenic |
| TGAGGAGGTTGAAAGGATTG+CGG | 0.659256 | 1_1:-61230376 | None:intergenic |
| TAGCCATAACTGTAATAGGG+AGG | 0.673963 | 1_1:-61230501 | None:intergenic |
| ATTCACTCCAGATCGGTGAG+TGG | 0.692086 | 1_1:+61230253 | Msa0033630:CDS |
| ATAACTGTAATAGGGAGGAG+GGG | 0.695524 | 1_1:-61230496 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAGTAGCCATAACTGTAATA+GGG | - | chr1_1:61230507-61230526 | None:intergenic | 30.0% | |
| TTAGTAGCCATAACTGTAAT+AGG | - | chr1_1:61230508-61230527 | None:intergenic | 30.0% | |
| ! | TTGTTTGTTTGGTGGAAGTT+GGG | - | chr1_1:61230300-61230319 | None:intergenic | 35.0% |
| ! | ATTGTTTGTTTGGTGGAAGT+TGG | - | chr1_1:61230301-61230320 | None:intergenic | 35.0% |
| TTCGGTCATGATTGTTTGTT+TGG | - | chr1_1:61230311-61230330 | None:intergenic | 35.0% | |
| ! | TGTTTGTTTGGTGGAAGTTG+GGG | - | chr1_1:61230299-61230318 | None:intergenic | 40.0% |
| GGTCATGATTGTTTGTTTGG+TGG | - | chr1_1:61230308-61230327 | None:intergenic | 40.0% | |
| CAAACAATCATGACCGAACA+CGG | + | chr1_1:61230313-61230332 | Msa0033630:CDS | 40.0% | |
| GGCAAACTCAAATCCTACTT+CGG | + | chr1_1:61230334-61230353 | Msa0033630:CDS | 40.0% | |
| AATTGGAAAGACTCGTACTG+AGG | - | chr1_1:61230397-61230416 | None:intergenic | 40.0% | |
| ATAACTGTAATAGGGAGGAG+GGG | - | chr1_1:61230499-61230518 | None:intergenic | 40.0% | |
| CATAACTGTAATAGGGAGGA+GGG | - | chr1_1:61230500-61230519 | None:intergenic | 40.0% | |
| TAGCCATAACTGTAATAGGG+AGG | - | chr1_1:61230504-61230523 | None:intergenic | 40.0% | |
| ! | GTTTGTTTGGTGGAAGTTGG+GGG | - | chr1_1:61230298-61230317 | None:intergenic | 45.0% |
| ! | GGATTTGAGTTTGCCGTGTT+CGG | - | chr1_1:61230329-61230348 | None:intergenic | 45.0% |
| TGAGGAGGTTGAAAGGATTG+CGG | - | chr1_1:61230379-61230398 | None:intergenic | 45.0% | |
| CCATAACTGTAATAGGGAGG+AGG | - | chr1_1:61230501-61230520 | None:intergenic | 45.0% | |
| CCTCCTCCCTATTACAGTTA+TGG | + | chr1_1:61230498-61230517 | Msa0033630:CDS | 45.0% | |
| ATTCACTCCAGATCGGTGAG+TGG | + | chr1_1:61230253-61230272 | Msa0033630:CDS | 50.0% | |
| TTTGGTGGAAGTTGGGGGTT+AGG | - | chr1_1:61230293-61230312 | None:intergenic | 50.0% | |
| CTCGTACTGAGGAGGTTGAA+AGG | - | chr1_1:61230386-61230405 | None:intergenic | 50.0% | |
| TGGAAAGACTCGTACTGAGG+AGG | - | chr1_1:61230394-61230413 | None:intergenic | 50.0% | |
| AGGGTGAAGAGGTGGGAAAT+TGG | - | chr1_1:61230414-61230433 | None:intergenic | 50.0% | |
| AAGGGTTAGGGTGAAGAGGT+GGG | - | chr1_1:61230421-61230440 | None:intergenic | 50.0% | |
| TAAGGGTTAGGGTGAAGAGG+TGG | - | chr1_1:61230422-61230441 | None:intergenic | 50.0% | |
| AACGAAGCCACTCACCGATC+TGG | - | chr1_1:61230263-61230282 | None:intergenic | 55.0% | |
| GTGGAAGTTGGGGGTTAGGA+GGG | - | chr1_1:61230289-61230308 | None:intergenic | 55.0% | |
| GGTTGAAAGGATTGCGGCGA+CGG | - | chr1_1:61230373-61230392 | None:intergenic | 55.0% | |
| GGGTAAGGGTTAGGGTGAAG+AGG | - | chr1_1:61230425-61230444 | None:intergenic | 55.0% | |
| ACTGTAATAGGGAGGAGGGG+CGG | - | chr1_1:61230496-61230515 | None:intergenic | 55.0% | |
| GGTGGAAGTTGGGGGTTAGG+AGG | - | chr1_1:61230290-61230309 | None:intergenic | 60.0% | |
| TGAAAGGATTGCGGCGACGG+CGG | - | chr1_1:61230370-61230389 | None:intergenic | 60.0% | |
| !! | GATGGCGGGGGTAAGGGTTA+GGG | - | chr1_1:61230433-61230452 | None:intergenic | 60.0% |
| CTAACCCTTACCCCCGCCAT+CGG | + | chr1_1:61230432-61230451 | Msa0033630:CDS | 60.0% | |
| !! | GAAACCGATGGCGGGGGTAA+GGG | - | chr1_1:61230439-61230458 | None:intergenic | 60.0% |
| TAATAGGGAGGAGGGGCGGA+GGG | - | chr1_1:61230492-61230511 | None:intergenic | 60.0% | |
| !! | CGATGGCGGGGGTAAGGGTT+AGG | - | chr1_1:61230434-61230453 | None:intergenic | 65.0% |
| !! | GGAAACCGATGGCGGGGGTA+AGG | - | chr1_1:61230440-61230459 | None:intergenic | 65.0% |
| ! | GTGAGGGAAACCGATGGCGG+GGG | - | chr1_1:61230445-61230464 | None:intergenic | 65.0% |
| GGTGAGGGAAACCGATGGCG+GGG | - | chr1_1:61230446-61230465 | None:intergenic | 65.0% | |
| GGGTGAGGGAAACCGATGGC+GGG | - | chr1_1:61230447-61230466 | None:intergenic | 65.0% | |
| GGGGTGAGGGAAACCGATGG+CGG | - | chr1_1:61230448-61230467 | None:intergenic | 65.0% | |
| GTCGGGGTGAGGGAAACCGA+TGG | - | chr1_1:61230451-61230470 | None:intergenic | 65.0% | |
| TCGGATCCAGGAGGACGGTC+GGG | - | chr1_1:61230468-61230487 | None:intergenic | 65.0% | |
| GTCGGATCCAGGAGGACGGT+CGG | - | chr1_1:61230469-61230488 | None:intergenic | 65.0% | |
| AGGGGTCGGATCCAGGAGGA+CGG | - | chr1_1:61230473-61230492 | None:intergenic | 65.0% | |
| AATAGGGAGGAGGGGCGGAG+GGG | - | chr1_1:61230491-61230510 | None:intergenic | 65.0% | |
| GTAATAGGGAGGAGGGGCGG+AGG | - | chr1_1:61230493-61230512 | None:intergenic | 65.0% | |
| CGGAGGAGGGTGGCCGAAGT+AGG | - | chr1_1:61230350-61230369 | None:intergenic | 70.0% | |
| ATTGCGGCGACGGCGGAGGA+GGG | - | chr1_1:61230363-61230382 | None:intergenic | 70.0% | |
| AAGGATTGCGGCGACGGCGG+AGG | - | chr1_1:61230367-61230386 | None:intergenic | 70.0% | |
| CAGGAGGACGGTCGGGGTGA+GGG | - | chr1_1:61230461-61230480 | None:intergenic | 70.0% | |
| CGGATCCAGGAGGACGGTCG+GGG | - | chr1_1:61230467-61230486 | None:intergenic | 70.0% | |
| GATTGCGGCGACGGCGGAGG+AGG | - | chr1_1:61230364-61230383 | None:intergenic | 75.0% | |
| CCAGGAGGACGGTCGGGGTG+AGG | - | chr1_1:61230462-61230481 | None:intergenic | 75.0% | |
| CCTCACCCCGACCGTCCTCC+TGG | + | chr1_1:61230459-61230478 | Msa0033630:CDS | 75.0% | |
| GCGGAGGGGTCGGATCCAGG+AGG | - | chr1_1:61230477-61230496 | None:intergenic | 75.0% | |
| ! | GGGGCGGAGGGGTCGGATCC+AGG | - | chr1_1:61230480-61230499 | None:intergenic | 80.0% |
| ! | GGGAGGAGGGGCGGAGGGGT+CGG | - | chr1_1:61230487-61230506 | None:intergenic | 80.0% |
| ! | GCGGCGACGGCGGAGGAGGG+TGG | - | chr1_1:61230360-61230379 | None:intergenic | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 61230249 | 61230527 | 61230249 | ID=Msa0033630;Name=Msa0033630 |
| chr1_1 | mRNA | 61230249 | 61230527 | 61230249 | ID=Msa0033630-mRNA-1;Parent=Msa0033630;Name=Msa0033630-mRNA-1;_AED=0.43;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|92 |
| chr1_1 | exon | 61230249 | 61230527 | 61230249 | ID=Msa0033630-mRNA-1:exon:19218;Parent=Msa0033630-mRNA-1 |
| chr1_1 | CDS | 61230249 | 61230527 | 61230249 | ID=Msa0033630-mRNA-1:cds;Parent=Msa0033630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
