AlfalfaGEDB | Msa0013670

M. sativa cultivar zhongmu-4 / Msa0013670

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0013670	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013670	Msa0062600	0.847397	1.186912e-59	-8.615850e-47
Msa0013670	Msa0107120	0.852694	3.901293e-61	-8.615850e-47
Msa0013670	Msa0107180	0.844839	5.894405e-59	-8.615850e-47
Msa0013670	Msa0215680	0.845637	3.587634e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0013670	MtrunA17_Chr1g0163201	63.446	383	80	5	18	341	17	398	9.38e-152	432
Msa0013670	MtrunA17_Chr1g0163181	57.177	418	82	7	19	341	20	435	2.45e-150	430
Msa0013670	MtrunA17_Chr1g0163251	49.828	291	107	5	19	288	20	292	2.53e-85	263
Msa0013670	MtrunA17_Chr1g0163151	57.658	222	42	2	167	336	1	222	5.31e-78	238
Msa0013670	MtrunA17_Chr1g0163111	53.648	233	43	6	167	337	1	230	2.82e-70	219
Msa0013670	MtrunA17_Chr1g0163231	43.706	286	129	9	8	277	12	281	1.37e-66	211
Msa0013670	MtrunA17_Chr1g0163261	40.816	294	132	6	14	284	18	292	1.73e-65	209
Msa0013670	MtrunA17_Chr1g0171611	44.406	286	113	10	9	277	13	269	8.96e-65	206
Msa0013670	MtrunA17_Chr1g0163191	71.724	145	34	2	19	161	20	159	1.06e-54	176
Msa0013670	MtrunA17_Chr1g0163241	63.194	144	34	3	18	158	21	148	8.64e-51	166

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 54 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGCAAAGTGGAGAT+TGG	0.431700	1_1:-21079883	Msa0013670:CDS
AAATACCAAAGCAAACTCAA+AGG	0.654830	1_1:-21081719	Msa0013670:CDS
AAGAGATATCCATATTTGAA+AGG	0.520085	1_1:+21081996	None:intergenic
AAGTGATGAATCAAGTGATG+AGG	0.567308	1_1:-21081802	Msa0013670:CDS
AATATTGTATGGGTGGTGGA+TGG	0.587908	1_1:-21081021	Msa0013670:CDS
AATGGCAAGAGAAAATTCTC+AGG	0.523324	1_1:-21081690	Msa0013670:intron
ACTTACAAAGAAATAGCCAT+TGG	0.583056	1_1:+21081230	None:intergenic
ACTTTCTTCACCTTCTTCAA+TGG	0.380996	1_1:+21081776	None:intergenic
AGAAAACCTAGTGATTTCTT+TGG	0.323706	1_1:+21080881	None:intergenic
AGAAATATGTGGAGAAATAT+TGG	0.281812	1_1:-21082136	Msa0013670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAATGAAACATTCAATGACT+CGG	-	chr1_1:21080481-21080500	Msa0013670:intron	30.0%
GCATACATCAAAAAACACAA+TGG	+	chr1_1:21080677-21080696	None:intergenic	30.0%
AAGATGATCCTATGAAAATC+TGG	+	chr1_1:21081028-21081047	None:intergenic	30.0%
TTTGAATTTCTTTGTTGCAG+AGG	-	chr1_1:21081196-21081215	Msa0013670:intron	30.0%
TTGAATTTCTTTGTTGCAGA+GGG	-	chr1_1:21081197-21081216	Msa0013670:intron	30.0%
TATGTGGAGAAATATTGGAA+AGG	-	chr1_1:21081231-21081250	Msa0013670:intron	30.0%
AAATACCAAAGCAAACTCAA+AGG	-	chr1_1:21081643-21081662	Msa0013670:intron	30.0%
CATTAACATCTTCAGATCTT+AGG	+	chr1_1:21082073-21082092	None:intergenic	30.0%
ACTTACAAAGAAATAGCCAT+TGG	+	chr1_1:21082135-21082154	None:intergenic	30.0%
TAAACCATTACTTGAAACAG+TGG	-	chr1_1:21082203-21082222	Msa0013670:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	21079804	21083580	21079804	ID=Msa0013670;Name=Msa0013670
chr1_1	mRNA	21079804	21083580	21079804	ID=Msa0013670-mRNA-1;Parent=Msa0013670;Name=Msa0013670-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|1\|0.28\|0.12\|8\|0\|341
chr1_1	exon	21083527	21083580	21083527	ID=Msa0013670-mRNA-1:exon:7489;Parent=Msa0013670-mRNA-1
chr1_1	exon	21081949	21082168	21081949	ID=Msa0013670-mRNA-1:exon:7488;Parent=Msa0013670-mRNA-1
chr1_1	exon	21081691	21081897	21081691	ID=Msa0013670-mRNA-1:exon:7487;Parent=Msa0013670-mRNA-1
chr1_1	exon	21081236	21081354	21081236	ID=Msa0013670-mRNA-1:exon:7486;Parent=Msa0013670-mRNA-1
chr1_1	exon	21080982	21081161	21080982	ID=Msa0013670-mRNA-1:exon:7485;Parent=Msa0013670-mRNA-1
chr1_1	exon	21080888	21080966	21080888	ID=Msa0013670-mRNA-1:exon:7484;Parent=Msa0013670-mRNA-1
chr1_1	exon	21080263	21080297	21080263	ID=Msa0013670-mRNA-1:exon:7483;Parent=Msa0013670-mRNA-1
chr1_1	exon	21079804	21079932	21079804	ID=Msa0013670-mRNA-1:exon:7482;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21083527	21083580	21083527	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21081949	21082168	21081949	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21081691	21081897	21081691	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21081236	21081354	21081236	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21080982	21081161	21080982	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21080888	21080966	21080888	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21080263	21080297	21080263	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1
chr1_1	CDS	21079804	21079932	21079804	ID=Msa0013670-mRNA-1:cds;Parent=Msa0013670-mRNA-1

Gene Sequence

>Msa0013670

ATGCGAGCTGGTTGGAAGTCGTTTGTGCAGGAATATAACTTGCAAGCTGGTGATAGGGTTCCAAAGAAATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAGTTTCCTAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAATTTTGTAAAGCCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCTGAAGACACAGAAGAAGGTAAAGAAAGAGATGAATCAAGTGATGATGAAGTCACAAAAGAAGCTCAAGAAAGTGATGAATCAAGTGATGAGGAAGCCATTGAAGAAGGTGAAGAAAGTGATGAAGAGAGTAATGAGAGTTCAGATGAGGTTGAAATACCAAAGCAAACTCAAAGGACCAGAAATGGCAAGAGAAAATTCTCAGGTAGTAACAAAGTGGCCATGGTAAAGAAGGCTAGAAAATGCCTAAGATCTGAAGATGTTAATGAGAATCCCTCTTTTGAAGTTATAATGTCACAATCTTACGCCAATGGCTATTTCTTTTGGATACCAAATGAGTTTTCAAGAGAACACTTGAAAGATTTTAATGGAACTACAACAATAAGGGTTGGTAATGAAAGGAGTGTGGAAGTGAGTGTGAGGTATTATGATACCCAGAAAAAATATTGTATGGGTGGTGGATGGAAAATATTCAGGCAAAAATATAATTTGCAAGTTGATGATATGATTCGACGGAGACCAATTTCATTCAATGTTACTATTACTCGTGCCAAAAACGAACCAAAGCCAAAGAAATCACTAGAAGGTCGTGATGTGAAGGAGCACAAGACATTCAAGTTCGGTAAAGGTTGGAGAAAATTCATGAAAGAATGCAAAGTGGAGATTGGAGATACTTGTCTCTTCAAGTTAATTGATGAAAGAAATTTTGTGTTTGATGTTTCAATTGTAGGAAAGAATCCATTAGCT

Protein sequence

>Msa0013670

MRAGWKSFVQEYNLQAGDRVPKKYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWENFVKPFKYGYLLTFKFIGGSYFKVKIFGAEDTEEGKERDESSDDEVTKEAQESDESSDEEAIEEGEESDEESNESSDEVEIPKQTQRTRNGKRKFSGSNKVAMVKKARKCLRSEDVNENPSFEVIMSQSYANGYFFWIPNEFSREHLKDFNGTTTIRVGNERSVEVSVRYYDTQKKYCMGGGWKIFRQKYNLQVDDMIRRRPISFNVTITRAKNEPKPKKSLEGRDVKEHKTFKFGKGWRKFMKECKVEIGDTCLFKLIDERNFVFDVSIVGKNPLA