Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026470 | Msa0026490 | 0.807288 | 5.439177e-50 | -8.615850e-47 |
| Msa0026470 | Msa0145280 | 0.854102 | 1.539475e-61 | -8.615850e-47 |
| Msa0026470 | Msa0145410 | 0.801800 | 7.590391e-49 | -8.615850e-47 |
| Msa0026470 | Msa0165540 | 0.923372 | 2.895730e-89 | -8.615850e-47 |
| Msa0026470 | Msa1043790 | 0.815033 | 1.137181e-51 | -8.615850e-47 |
| Msa0026470 | Msa1043810 | 0.877516 | 5.902678e-69 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0026470 | MtrunA17_Chr1g0153601 | 92.632 | 95 | 7 | 0 | 1 | 95 | 1 | 95 | 3.61e-61 | 181 |
| Msa0026470 | MtrunA17_Chr1g0153581 | 89.474 | 95 | 10 | 0 | 1 | 95 | 1 | 95 | 2.80e-58 | 174 |
| Msa0026470 | MtrunA17_Chr1g0153561 | 84.211 | 95 | 15 | 0 | 1 | 95 | 1 | 95 | 1.20e-54 | 164 |
| Msa0026470 | MtrunA17_Chr1g0153611 | 65.957 | 94 | 31 | 1 | 1 | 94 | 1 | 93 | 2.38e-39 | 125 |
| Msa0026470 | MtrunA17_Chr1g0153551 | 46.000 | 100 | 49 | 1 | 1 | 95 | 1 | 100 | 6.52e-20 | 77.0 |
| Msa0026470 | MtrunA17_Chr1g0153541 | 40.777 | 103 | 53 | 1 | 1 | 95 | 1 | 103 | 3.39e-17 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCTCCTTAGCCGTGGTTT+TGG | 0.295002 | 1_1:-50317157 | Msa0026470:CDS |
| TGTTCCTTTCGAAGTTGATT+TGG | 0.344741 | 1_1:+50317365 | None:intergenic |
| AGCTTTCATGAAACAAAATA+TGG | 0.357016 | 1_1:+50317234 | None:intergenic |
| ACTGATTTGTCCGTCTTTGT+TGG | 0.364609 | 1_1:+50317207 | None:intergenic |
| CAAAATATGGCTCTCCATCC+AGG | 0.411421 | 1_1:+50317247 | None:intergenic |
| TGGTCCATAGGCACCGGCTT+TGG | 0.436522 | 1_1:+50317385 | None:intergenic |
| CAAACCAAAGCCGGTGCCTA+TGG | 0.455404 | 1_1:-50317389 | Msa0026470:CDS |
| AATGCCTGTGTTTATCCCTT+CGG | 0.462697 | 1_1:-50317413 | Msa0026470:CDS |
| ACCGGCTTTGGTTTGTCCGA+AGG | 0.472684 | 1_1:+50317397 | None:intergenic |
| CCGGCTTTGGTTTGTCCGAA+GGG | 0.495436 | 1_1:+50317398 | None:intergenic |
| TAGGTTCTTACTACCCTGGA+TGG | 0.528156 | 1_1:-50317261 | Msa0026470:CDS |
| TCGAAGTTGATTTGGTCCAT+AGG | 0.530320 | 1_1:+50317373 | None:intergenic |
| GATTTAGGTTCTTACTACCC+TGG | 0.543944 | 1_1:-50317265 | Msa0026470:CDS |
| TCTTGCCAAAACCACGGCTA+AGG | 0.544398 | 1_1:+50317152 | None:intergenic |
| CCCTTCGGACAAACCAAAGC+CGG | 0.550311 | 1_1:-50317398 | Msa0026470:CDS |
| TTGATTTGGTCCATAGGCAC+CGG | 0.550954 | 1_1:+50317379 | None:intergenic |
| AAAATATGGCTCTCCATCCA+GGG | 0.594858 | 1_1:+50317248 | None:intergenic |
| TGGACCAAATCAACTTCGAA+AGG | 0.599482 | 1_1:-50317369 | Msa0026470:CDS |
| GCTGATTATCTCCTTAGCCG+TGG | 0.615853 | 1_1:-50317163 | Msa0026470:CDS |
| AAAGCTGATGCCAACAAAGA+CGG | 0.620958 | 1_1:-50317217 | Msa0026470:CDS |
| ATTAGTTCTTGCCAAAACCA+CGG | 0.621014 | 1_1:+50317146 | None:intergenic |
| AAGGCAGATATCAATAAAGA+CGG | 0.625573 | 1_1:-50317325 | Msa0026470:CDS |
| AACAAAGACGGACAAATCAG+TGG | 0.638335 | 1_1:-50317205 | Msa0026470:CDS |
| TTGTCCGAAGGGATAAACAC+AGG | 0.660183 | 1_1:+50317409 | None:intergenic |
| AGACGGACAAATCAGTGGCG+AGG | 0.670203 | 1_1:-50317200 | Msa0026470:CDS |
| TAAAGACGGTCGTTACACAA+AGG | 0.678917 | 1_1:-50317311 | Msa0026470:CDS |
| AGACGGTCGTTACACAAAGG+AGG | 0.680084 | 1_1:-50317308 | Msa0026470:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGGAACATTATCGAAAAAT+TGG | - | chr1_1:50317209-50317228 | Msa0026470:CDS | 25.0% |
| ! | CATTATCGAAAAATTGGAAA+AGG | - | chr1_1:50317215-50317234 | Msa0026470:CDS | 25.0% |
| !!! | AAAAAAGCTCTAAAGGATTT+AGG | - | chr1_1:50317279-50317298 | Msa0026470:CDS | 25.0% |
| ! | AGCTTTCATGAAACAAAATA+TGG | + | chr1_1:50317328-50317347 | None:intergenic | 25.0% |
| AAGGCAGATATCAATAAAGA+CGG | - | chr1_1:50317234-50317253 | Msa0026470:CDS | 30.0% | |
| ! | TGTTCCTTTCGAAGTTGATT+TGG | + | chr1_1:50317197-50317216 | None:intergenic | 35.0% |
| ! | GGAACTCAAAAAAGCTCTAA+AGG | - | chr1_1:50317272-50317291 | Msa0026470:CDS | 35.0% |
| !! | TCGAAGTTGATTTGGTCCAT+AGG | + | chr1_1:50317189-50317208 | None:intergenic | 40.0% |
| TGGACCAAATCAACTTCGAA+AGG | - | chr1_1:50317190-50317209 | Msa0026470:CDS | 40.0% | |
| TAAAGACGGTCGTTACACAA+AGG | - | chr1_1:50317248-50317267 | Msa0026470:CDS | 40.0% | |
| ! | GATTTAGGTTCTTACTACCC+TGG | - | chr1_1:50317294-50317313 | Msa0026470:CDS | 40.0% |
| AAAATATGGCTCTCCATCCA+GGG | + | chr1_1:50317314-50317333 | None:intergenic | 40.0% | |
| AAAGCTGATGCCAACAAAGA+CGG | - | chr1_1:50317342-50317361 | Msa0026470:CDS | 40.0% | |
| !! | ACTGATTTGTCCGTCTTTGT+TGG | + | chr1_1:50317355-50317374 | None:intergenic | 40.0% |
| AACAAAGACGGACAAATCAG+TGG | - | chr1_1:50317354-50317373 | Msa0026470:CDS | 40.0% | |
| TTGTCCGAAGGGATAAACAC+AGG | + | chr1_1:50317153-50317172 | None:intergenic | 45.0% | |
| !! | TTGATTTGGTCCATAGGCAC+CGG | + | chr1_1:50317183-50317202 | None:intergenic | 45.0% |
| TAGGTTCTTACTACCCTGGA+TGG | - | chr1_1:50317298-50317317 | Msa0026470:CDS | 45.0% | |
| CAAAATATGGCTCTCCATCC+AGG | + | chr1_1:50317315-50317334 | None:intergenic | 45.0% | |
| ! | TATCTCCTTAGCCGTGGTTT+TGG | - | chr1_1:50317402-50317421 | Msa0026470:CDS | 45.0% |
| AGACGGTCGTTACACAAAGG+AGG | - | chr1_1:50317251-50317270 | Msa0026470:CDS | 50.0% | |
| GCTGATTATCTCCTTAGCCG+TGG | - | chr1_1:50317396-50317415 | Msa0026470:CDS | 50.0% | |
| TCTTGCCAAAACCACGGCTA+AGG | + | chr1_1:50317410-50317429 | None:intergenic | 50.0% | |
| !! | CCGGCTTTGGTTTGTCCGAA+GGG | + | chr1_1:50317164-50317183 | None:intergenic | 55.0% |
| CCCTTCGGACAAACCAAAGC+CGG | - | chr1_1:50317161-50317180 | Msa0026470:CDS | 55.0% | |
| !! | ACCGGCTTTGGTTTGTCCGA+AGG | + | chr1_1:50317165-50317184 | None:intergenic | 55.0% |
| CAAACCAAAGCCGGTGCCTA+TGG | - | chr1_1:50317170-50317189 | Msa0026470:CDS | 55.0% | |
| AGACGGACAAATCAGTGGCG+AGG | - | chr1_1:50317359-50317378 | Msa0026470:CDS | 55.0% | |
| !! | TGGTCCATAGGCACCGGCTT+TGG | + | chr1_1:50317177-50317196 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 50317147 | 50317434 | 50317147 | ID=Msa0026470;Name=Msa0026470 |
| chr1_1 | mRNA | 50317147 | 50317434 | 50317147 | ID=Msa0026470-mRNA-1;Parent=Msa0026470;Name=Msa0026470-mRNA-1;_AED=0.34;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|95 |
| chr1_1 | exon | 50317147 | 50317434 | 50317147 | ID=Msa0026470-mRNA-1:exon:14629;Parent=Msa0026470-mRNA-1 |
| chr1_1 | CDS | 50317147 | 50317434 | 50317147 | ID=Msa0026470-mRNA-1:cds;Parent=Msa0026470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
