Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0145410 | G7I3M4 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 1.81e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026470 | Msa0145410 | 0.801800 | 7.590391e-49 | -8.615850e-47 |
| Msa0026490 | Msa0145410 | 0.984195 | 7.380761e-160 | -8.615850e-47 |
| Msa0037700 | Msa0145410 | 0.814720 | 1.334056e-51 | -8.615850e-47 |
| Msa0057520 | Msa0145410 | 0.809817 | 1.568415e-50 | -8.615850e-47 |
| Msa0063350 | Msa0145410 | -0.808381 | 3.185279e-50 | -8.615850e-47 |
| Msa0133130 | Msa0145410 | 0.801964 | 7.023902e-49 | -8.615850e-47 |
| Msa0145280 | Msa0145410 | 0.810182 | 1.308802e-50 | -8.615850e-47 |
| Msa0145310 | Msa0145410 | 0.957613 | 1.777803e-115 | -8.615850e-47 |
| Msa0145410 | Msa0165540 | 0.807140 | 5.846167e-50 | -8.615850e-47 |
| Msa0145410 | Msa0173990 | 0.805501 | 1.294795e-49 | -8.615850e-47 |
| Msa0145410 | Msa0178860 | -0.806049 | 9.935934e-50 | -8.615850e-47 |
| Msa0145410 | Msa0223740 | 0.811333 | 7.376540e-51 | -8.615850e-47 |
| Msa0145410 | Msa0302580 | -0.804666 | 1.935928e-49 | -8.615850e-47 |
| Msa0145410 | Msa0309550 | 0.821356 | 4.216524e-53 | -8.615850e-47 |
| Msa0145410 | Msa0389810 | 0.807913 | 4.006368e-50 | -8.615850e-47 |
| Msa0145410 | Msa0431060 | 0.800578 | 1.349783e-48 | -8.615850e-47 |
| Msa0145410 | Msa0466060 | 0.806861 | 6.698292e-50 | -8.615850e-47 |
| Msa0145410 | Msa0466390 | 0.800633 | 1.315160e-48 | -8.615850e-47 |
| Msa0145410 | Msa0484780 | 0.801697 | 7.968310e-49 | -8.615850e-47 |
| Msa0145410 | Msa0534730 | 0.806206 | 9.203818e-50 | -8.615850e-47 |
| Msa0145410 | Msa0536220 | 0.800980 | 1.117356e-48 | -8.615850e-47 |
| Msa0145410 | Msa0631200 | 0.840410 | 8.842472e-58 | -8.615850e-47 |
| Msa0145410 | Msa0669440 | 0.809497 | 1.837421e-50 | -8.615850e-47 |
| Msa0145410 | Msa0982270 | 0.807544 | 4.800253e-50 | -8.615850e-47 |
| Msa0145410 | Msa1000020 | 0.808810 | 2.579147e-50 | -8.615850e-47 |
| Msa0145410 | Msa1043790 | 0.947658 | 4.377110e-106 | -8.615850e-47 |
| Msa0145410 | Msa1044420 | 0.807348 | 5.282340e-50 | -8.615850e-47 |
| Msa0145410 | Msa1073070 | -0.817815 | 2.711706e-52 | -8.615850e-47 |
| Msa0145410 | Msa1078710 | 0.814069 | 1.858440e-51 | -8.615850e-47 |
| Msa0145410 | Msa1082500 | 0.801459 | 8.915846e-49 | -8.615850e-47 |
| Msa0145410 | Msa1124540 | -0.814451 | 1.530544e-51 | -8.615850e-47 |
| Msa0145410 | Msa1133710 | 0.824334 | 8.539016e-54 | -8.615850e-47 |
| Msa0145410 | Msa1183100 | -0.835710 | 1.432435e-56 | -8.615850e-47 |
| Msa0145410 | Msa1214880 | 0.801305 | 9.587266e-49 | -8.615850e-47 |
| Msa0145410 | Msa1217810 | 0.800904 | 1.158079e-48 | -8.615850e-47 |
| Msa0145410 | Msa1233500 | 0.809253 | 2.073700e-50 | -8.615850e-47 |
| Msa0145410 | Msa1305190 | 0.833371 | 5.542163e-56 | -8.615850e-47 |
| Msa0145410 | Msa1305340 | 0.830273 | 3.221591e-55 | -8.615850e-47 |
| Msa0145410 | Msa1322190 | 0.829626 | 4.632832e-55 | -8.615850e-47 |
| Msa0145410 | Msa1444250 | -0.800206 | 1.607017e-48 | -8.615850e-47 |
| Msa0145410 | Msa1451620 | -0.810098 | 1.364347e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0145410 | MtrunA17_Chr1g0153581 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 3.49e-61 | 181 |
| Msa0145410 | MtrunA17_Chr1g0153601 | 89.474 | 95 | 6 | 1 | 1 | 91 | 1 | 95 | 3.08e-55 | 166 |
| Msa0145410 | MtrunA17_Chr1g0153561 | 86.316 | 95 | 9 | 1 | 1 | 91 | 1 | 95 | 1.54e-54 | 164 |
| Msa0145410 | MtrunA17_Chr1g0153611 | 65.591 | 93 | 29 | 1 | 1 | 90 | 1 | 93 | 1.16e-38 | 124 |
| Msa0145410 | MtrunA17_Chr1g0153551 | 45.000 | 100 | 46 | 1 | 1 | 91 | 1 | 100 | 2.50e-19 | 75.5 |
| Msa0145410 | MtrunA17_Chr1g0153541 | 43.678 | 87 | 45 | 1 | 5 | 91 | 21 | 103 | 2.01e-16 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGATTAGTCCGTCTTTAT+TGG | 0.241517 | 1_4:-5070878 | None:intergenic |
| TATCTCCTTACCCGTGGTTT+TGG | 0.273881 | 1_4:+5070928 | Msa0145410:CDS |
| TGATCCATAGACACCCTCTT+TGG | 0.332546 | 1_4:-5070700 | None:intergenic |
| TATCCCAAAGAGGGTGTCTA+TGG | 0.383107 | 1_4:+5070696 | Msa0145410:CDS |
| GATCCATAGACACCCTCTTT+GGG | 0.423736 | 1_4:-5070699 | None:intergenic |
| CAACGATTGGCTCTCCATCC+AGG | 0.437823 | 1_4:-5070838 | None:intergenic |
| AGCTTTCATCAAACAACGAT+TGG | 0.498009 | 1_4:-5070851 | None:intergenic |
| TCTTGCCAAAACCACGGGTA+AGG | 0.519368 | 1_4:-5070933 | None:intergenic |
| CGATGGTCGTTACACAAAGA+AGG | 0.521782 | 1_4:+5070777 | Msa0145410:CDS |
| TAGGTTCTTACTACCCTGGA+TGG | 0.528156 | 1_4:+5070824 | Msa0145410:CDS |
| TTAGTTCTTGCCAAAACCAC+GGG | 0.528157 | 1_4:-5070938 | None:intergenic |
| TGGATCAAATCAACTTTGAA+AGG | 0.536831 | 1_4:+5070716 | Msa0145410:CDS |
| GATTTAGGTTCTTACTACCC+TGG | 0.543944 | 1_4:+5070820 | Msa0145410:CDS |
| AACGATTGGCTCTCCATCCA+GGG | 0.580860 | 1_4:-5070837 | None:intergenic |
| ATTAGTTCTTGCCAAAACCA+CGG | 0.592102 | 1_4:-5070939 | None:intergenic |
| GCCTGTGTTTATCCCAAAGA+GGG | 0.602626 | 1_4:+5070687 | Msa0145410:CDS |
| ATTGATTATCTCCTTACCCG+TGG | 0.619921 | 1_4:+5070922 | Msa0145410:CDS |
| AAAGCTGATGCCAATAAAGA+CGG | 0.628000 | 1_4:+5070868 | Msa0145410:CDS |
| TGCCTGTGTTTATCCCAAAG+AGG | 0.630941 | 1_4:+5070686 | Msa0145410:CDS |
| ACCCTCTTTGGGATAAACAC+AGG | 0.645492 | 1_4:-5070688 | None:intergenic |
| AGACGGACTAATCAGTGGCG+AGG | 0.659853 | 1_4:+5070885 | Msa0145410:CDS |
| AATAAAGACGGACTAATCAG+TGG | 0.668016 | 1_4:+5070880 | Msa0145410:CDS |
| AAGGCGGATATCAATAACGA+TGG | 0.697636 | 1_4:+5070760 | Msa0145410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGAACATTATAGAAAAAT+TGG | + | chr1_4:5070735-5070754 | Msa0145410:CDS | 20.0% |
| ! | CATTATAGAAAAATTGGAGA+AGG | + | chr1_4:5070741-5070760 | Msa0145410:CDS | 25.0% |
| !!! | AAAAAAGCTCTAAAGGATTT+AGG | + | chr1_4:5070805-5070824 | Msa0145410:CDS | 25.0% |
| TGGATCAAATCAACTTTGAA+AGG | + | chr1_4:5070716-5070735 | Msa0145410:CDS | 30.0% | |
| TATAGAAAAATTGGAGAAGG+CGG | + | chr1_4:5070744-5070763 | Msa0145410:CDS | 30.0% | |
| ! | GGAACTCAAAAAAGCTCTAA+AGG | + | chr1_4:5070798-5070817 | Msa0145410:CDS | 35.0% |
| AGCTTTCATCAAACAACGAT+TGG | - | chr1_4:5070854-5070873 | None:intergenic | 35.0% | |
| AAAGCTGATGCCAATAAAGA+CGG | + | chr1_4:5070868-5070887 | Msa0145410:CDS | 35.0% | |
| ! | ACTGATTAGTCCGTCTTTAT+TGG | - | chr1_4:5070881-5070900 | None:intergenic | 35.0% |
| !! | AATAAAGACGGACTAATCAG+TGG | + | chr1_4:5070880-5070899 | Msa0145410:CDS | 35.0% |
| AAGGCGGATATCAATAACGA+TGG | + | chr1_4:5070760-5070779 | Msa0145410:CDS | 40.0% | |
| ! | GATTTAGGTTCTTACTACCC+TGG | + | chr1_4:5070820-5070839 | Msa0145410:CDS | 40.0% |
| ATTGATTATCTCCTTACCCG+TGG | + | chr1_4:5070922-5070941 | Msa0145410:CDS | 40.0% | |
| ! | TTAGTTCTTGCCAAAACCAC+GGG | - | chr1_4:5070941-5070960 | None:intergenic | 40.0% |
| TGCCTGTGTTTATCCCAAAG+AGG | + | chr1_4:5070686-5070705 | Msa0145410:CDS | 45.0% | |
| GCCTGTGTTTATCCCAAAGA+GGG | + | chr1_4:5070687-5070706 | Msa0145410:CDS | 45.0% | |
| !! | ACCCTCTTTGGGATAAACAC+AGG | - | chr1_4:5070691-5070710 | None:intergenic | 45.0% |
| !! | TATCCCAAAGAGGGTGTCTA+TGG | + | chr1_4:5070696-5070715 | Msa0145410:CDS | 45.0% |
| GATCCATAGACACCCTCTTT+GGG | - | chr1_4:5070702-5070721 | None:intergenic | 45.0% | |
| TGATCCATAGACACCCTCTT+TGG | - | chr1_4:5070703-5070722 | None:intergenic | 45.0% | |
| CGATGGTCGTTACACAAAGA+AGG | + | chr1_4:5070777-5070796 | Msa0145410:CDS | 45.0% | |
| TAGGTTCTTACTACCCTGGA+TGG | + | chr1_4:5070824-5070843 | Msa0145410:CDS | 45.0% | |
| ! | TATCTCCTTACCCGTGGTTT+TGG | + | chr1_4:5070928-5070947 | Msa0145410:CDS | 45.0% |
| ! | AACGATTGGCTCTCCATCCA+GGG | - | chr1_4:5070840-5070859 | None:intergenic | 50.0% |
| TCTTGCCAAAACCACGGGTA+AGG | - | chr1_4:5070936-5070955 | None:intergenic | 50.0% | |
| ! | CAACGATTGGCTCTCCATCC+AGG | - | chr1_4:5070841-5070860 | None:intergenic | 55.0% |
| !! | AGACGGACTAATCAGTGGCG+AGG | + | chr1_4:5070885-5070904 | Msa0145410:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 5070685 | 5070960 | 5070685 | ID=Msa0145410;Name=Msa0145410 |
| chr1_4 | mRNA | 5070685 | 5070960 | 5070685 | ID=Msa0145410-mRNA-1;Parent=Msa0145410;Name=Msa0145410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|91 |
| chr1_4 | exon | 5070685 | 5070960 | 5070685 | ID=Msa0145410-mRNA-1:exon:2072;Parent=Msa0145410-mRNA-1 |
| chr1_4 | CDS | 5070685 | 5070960 | 5070685 | ID=Msa0145410-mRNA-1:cds;Parent=Msa0145410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
